LOCUS       DACCPY010000001        72235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCPY010000001 DACCPY010000000
VERSION     DACCPY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.001"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(75..797)
                     /locus_tag="IVO69_000001"
     CDS             complement(75..797)
                     /locus_tag="IVO69_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7398815.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(787..1524)
                     /locus_tag="IVO69_000002"
     CDS             complement(787..1524)
                     /locus_tag="IVO69_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7398816.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1536..2528)
                     /locus_tag="IVO69_000003"
     CDS             complement(1536..2528)
                     /locus_tag="IVO69_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7398817.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2543..3286)
                     /locus_tag="IVO69_000004"
     CDS             complement(2543..3286)
                     /locus_tag="IVO69_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7398818.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3360..3464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3527..4849)
                     /locus_tag="IVO69_000005"
     CDS             complement(3527..4849)
                     /locus_tag="IVO69_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7398819.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4836..5801)
                     /locus_tag="IVO69_000006"
     CDS             complement(4836..5801)
                     /locus_tag="IVO69_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7398820.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5798..7336)
                     /locus_tag="IVO69_000007"
     CDS             complement(5798..7336)
                     /locus_tag="IVO69_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7398821.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7752..8039)
                     /locus_tag="IVO69_000008"
     CDS             complement(7752..8039)
                     /locus_tag="IVO69_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398822.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8173..8841
                     /locus_tag="IVO69_000009"
     CDS             8173..8841
                     /locus_tag="IVO69_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7398823.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9132..9872)
                     /locus_tag="IVO69_000010"
     CDS             complement(9132..9872)
                     /locus_tag="IVO69_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398824.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLRGLIDDQGNSLGENSYFK"
     gene            complement(10002..10949)
                     /locus_tag="IVO69_000011"
     CDS             complement(10002..10949)
                     /locus_tag="IVO69_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7398825.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11078..11842)
                     /locus_tag="IVO69_000012"
     CDS             complement(11078..11842)
                     /locus_tag="IVO69_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7398826.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(11846..12235)
                     /locus_tag="IVO69_000013"
     CDS             complement(11846..12235)
                     /locus_tag="IVO69_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398827.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12370..12912
                     /locus_tag="IVO69_000014"
     CDS             12370..12912
                     /locus_tag="IVO69_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7398828.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13060..13692
                     /locus_tag="IVO69_000015"
     CDS             13060..13692
                     /locus_tag="IVO69_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398829.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(13826..13951)
                     /locus_tag="IVO69_000016"
     CDS             complement(13826..13951)
                     /locus_tag="IVO69_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7398830.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14085..14978)
                     /locus_tag="IVO69_000017"
     CDS             complement(14085..14978)
                     /locus_tag="IVO69_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7398831.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15160..16086)
                     /locus_tag="IVO69_000018"
     CDS             complement(15160..16086)
                     /locus_tag="IVO69_000018"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7398832.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16171..16932)
                     /gene="pflA"
                     /locus_tag="IVO69_000019"
     CDS             complement(16171..16932)
                     /gene="pflA"
                     /locus_tag="IVO69_000019"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7398833.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17176..19407)
                     /gene="pflB"
                     /locus_tag="IVO69_000020"
     CDS             complement(17176..19407)
                     /gene="pflB"
                     /locus_tag="IVO69_000020"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7398834.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(19684..22134)
                     /gene="parC"
                     /locus_tag="IVO69_000021"
     CDS             complement(19684..22134)
                     /gene="parC"
                     /locus_tag="IVO69_000021"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7398835.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTNVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22146..24191)
                     /gene="parE"
                     /locus_tag="IVO69_000022"
     CDS             complement(22146..24191)
                     /gene="parE"
                     /locus_tag="IVO69_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7398836.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24357..24791)
                     /locus_tag="IVO69_000023"
     CDS             complement(24357..24791)
                     /locus_tag="IVO69_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7398837.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            24918..25556
                     /gene="plsY"
                     /locus_tag="IVO69_000024"
     CDS             24918..25556
                     /gene="plsY"
                     /locus_tag="IVO69_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7398838.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(25688..26563)
                     /locus_tag="IVO69_000025"
     CDS             complement(25688..26563)
                     /locus_tag="IVO69_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7398839.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(26645..27436)
                     /gene="codY"
                     /locus_tag="IVO69_000026"
     CDS             complement(26645..27436)
                     /gene="codY"
                     /locus_tag="IVO69_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7398840.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27454..28854)
                     /gene="hslU"
                     /locus_tag="IVO69_000027"
     CDS             complement(27454..28854)
                     /gene="hslU"
                     /locus_tag="IVO69_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7398841.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(28868..29416)
                     /gene="hslV"
                     /locus_tag="IVO69_000028"
     CDS             complement(28868..29416)
                     /gene="hslV"
                     /locus_tag="IVO69_000028"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7398842.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29453..30358)
                     /gene="xerC"
                     /locus_tag="IVO69_000029"
     CDS             complement(29453..30358)
                     /gene="xerC"
                     /locus_tag="IVO69_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7398843.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYCSFHPRA"
     gene            complement(30556..31932)
                     /gene="trmFO"
                     /locus_tag="IVO69_000030"
     CDS             complement(30556..31932)
                     /gene="trmFO"
                     /locus_tag="IVO69_000030"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7398844.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(31901..33979)
                     /gene="topA"
                     /locus_tag="IVO69_000031"
     CDS             complement(31901..33979)
                     /gene="topA"
                     /locus_tag="IVO69_000031"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7398845.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34101..34958)
                     /gene="dprA"
                     /locus_tag="IVO69_000032"
     CDS             complement(34101..34958)
                     /gene="dprA"
                     /locus_tag="IVO69_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7398846.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35014..35781)
                     /locus_tag="IVO69_000033"
     CDS             complement(35014..35781)
                     /locus_tag="IVO69_000033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7398847.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPCYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(35774..36634)
                     /gene="ylqF"
                     /locus_tag="IVO69_000034"
     CDS             complement(35774..36634)
                     /gene="ylqF"
                     /locus_tag="IVO69_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7398848.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            36894..37292
                     /locus_tag="IVO69_000035"
     CDS             36894..37292
                     /locus_tag="IVO69_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7398849.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37529..38071
                     /locus_tag="IVO69_000036"
     CDS             37529..38071
                     /locus_tag="IVO69_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7398850.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38154..38705)
                     /gene="lepB"
                     /locus_tag="IVO69_000037"
     CDS             complement(38154..38705)
                     /gene="lepB"
                     /locus_tag="IVO69_000037"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7398851.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(38857..39084)
                     /locus_tag="IVO69_000038"
     CDS             complement(38857..39084)
                     /locus_tag="IVO69_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7398852.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39081..39599)
                     /gene="msrA"
                     /locus_tag="IVO69_000039"
     CDS             complement(39081..39599)
                     /gene="msrA"
                     /locus_tag="IVO69_000039"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7398853.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(39619..40332)
                     /locus_tag="IVO69_000040"
     CDS             complement(39619..40332)
                     /locus_tag="IVO69_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7398854.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40335..41231)
                     /locus_tag="IVO69_000041"
     CDS             complement(40335..41231)
                     /locus_tag="IVO69_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7398855.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41389..42231)
                     /locus_tag="IVO69_000042"
     CDS             complement(41389..42231)
                     /locus_tag="IVO69_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7398856.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42363..43016
                     /locus_tag="IVO69_000043"
     CDS             42363..43016
                     /locus_tag="IVO69_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7398857.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43079..43606)
                     /locus_tag="IVO69_000044"
     CDS             complement(43079..43606)
                     /locus_tag="IVO69_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7398858.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(43626..44573)
                     /locus_tag="IVO69_000045"
     CDS             complement(43626..44573)
                     /locus_tag="IVO69_000045"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7398859.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(44585..46471)
                     /locus_tag="IVO69_000046"
     CDS             complement(44585..46471)
                     /locus_tag="IVO69_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7398860.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(46631..48709)
                     /locus_tag="IVO69_000047"
     CDS             complement(46631..48709)
                     /locus_tag="IVO69_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7398861.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(48722..48997)
                     /locus_tag="IVO69_000048"
     CDS             complement(48722..48997)
                     /locus_tag="IVO69_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398862.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49359..49814)
                     /locus_tag="IVO69_000049"
     CDS             complement(49359..49814)
                     /locus_tag="IVO69_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398863.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(49968..51176)
                     /locus_tag="IVO69_000050"
     CDS             complement(49968..51176)
                     /locus_tag="IVO69_000050"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7398864.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51288..51620)
                     /locus_tag="IVO69_000051"
     CDS             complement(51288..51620)
                     /locus_tag="IVO69_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7398865.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51768..52643
                     /locus_tag="IVO69_000052"
     CDS             51768..52643
                     /locus_tag="IVO69_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7398866.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(52725..53306)
                     /locus_tag="IVO69_000053"
     CDS             complement(52725..53306)
                     /locus_tag="IVO69_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7398867.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53312..54571)
                     /locus_tag="IVO69_000054"
     CDS             complement(53312..54571)
                     /locus_tag="IVO69_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7398868.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(54573..55619)
                     /locus_tag="IVO69_000055"
     CDS             complement(54573..55619)
                     /locus_tag="IVO69_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398869.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(55854..56129)
                     /locus_tag="IVO69_000056"
     CDS             complement(55854..56129)
                     /locus_tag="IVO69_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7398870.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56372..57682)
                     /locus_tag="IVO69_000057"
     CDS             complement(56372..57682)
                     /locus_tag="IVO69_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7398871.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(57864..59093)
                     /gene="rpsA"
                     /locus_tag="IVO69_000058"
     CDS             complement(57864..59093)
                     /gene="rpsA"
                     /locus_tag="IVO69_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7398872.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59228..59908)
                     /locus_tag="IVO69_000059"
     CDS             complement(59228..59908)
                     /locus_tag="IVO69_000059"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7398873.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     SITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(59957..60592)
                     /locus_tag="IVO69_000060"
     CDS             complement(59957..60592)
                     /locus_tag="IVO69_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7398874.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(60658..62061)
                     /locus_tag="IVO69_000061"
     CDS             complement(60658..62061)
                     /locus_tag="IVO69_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7398875.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62048..63079)
                     /locus_tag="IVO69_000062"
     CDS             complement(62048..63079)
                     /locus_tag="IVO69_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398876.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63110..63331
                     /locus_tag="IVO69_000063"
     CDS             63110..63331
                     /locus_tag="IVO69_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7398877.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63496..64233)
                     /locus_tag="IVO69_000064"
     CDS             complement(63496..64233)
                     /locus_tag="IVO69_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7398878.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64386..65726)
                     /locus_tag="IVO69_000065"
     CDS             complement(64386..65726)
                     /locus_tag="IVO69_000065"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7398879.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(65963..67486)
                     /locus_tag="IVO69_000066"
     CDS             complement(65963..67486)
                     /locus_tag="IVO69_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7398880.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(67864..68475)
                     /locus_tag="IVO69_000067"
     CDS             complement(67864..68475)
                     /locus_tag="IVO69_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7398881.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(68598..68723)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(68805..69521)
                     /locus_tag="IVO69_000068"
     CDS             complement(68805..69521)
                     /locus_tag="IVO69_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7398882.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69527..70096)
                     /gene="scpB"
                     /locus_tag="IVO69_000069"
     CDS             complement(69527..70096)
                     /gene="scpB"
                     /locus_tag="IVO69_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7398883.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70080..70877)
                     /locus_tag="IVO69_000070"
     CDS             complement(70080..70877)
                     /locus_tag="IVO69_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7398884.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(70847..71218)
                     /locus_tag="IVO69_000071"
     CDS             complement(70847..71218)
                     /locus_tag="IVO69_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7398885.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71237..72124)
                     /gene="xerD"
                     /locus_tag="IVO69_000072"
     CDS             complement(71237..72124)
                     /gene="xerD"
                     /locus_tag="IVO69_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7398886.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT        19924 a        15219 c        12311 g        24781 t
ORIGIN      
        1 attaatcaaa cagtttactt ttatacaaag aaagccaaaa agaggtgcga cactagttgt
       61 gtcacatctc ttttttattt acctaatcta atgaaacgtt ccaagttttg ataagtattt
      121 tttttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa ctgttcttca
      181 agcagtgaga cataatcttt ttctgaaaaa atccccccta aagcccactc tgctgcatgt
      241 agtccatccg tttcaataag taagtggtcg attgggactg ctttagctag ttgccagact
      301 gctggattct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc aatatagtct
      361 tgttgatagt ccaaagaaga ataccaatgg atcaaatagt gattagggta agcttggata
      421 taatttaaga tctctttttc gcatcctttt gtgtgcagta caacaggttt ctcatttatg
      481 gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg tacattcgtc
      541 caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa aggctcaact
      601 tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa ggcttgagtc
      661 ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca attgatgatc
      721 gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc taaatgacaa
      781 tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt gcttcaactc
      841 tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa tctcagcaat
      901 cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa ttgcttcttc
      961 gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct tcaacatcca
     1021 gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat caagaagcaa
     1081 gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca tcccgccaga
     1141 taattgttcc ggcaaaaaat gttcatagcc gtttaaccca aagatatcca gataatccct
     1201 tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa cgttttgata
     1261 gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg ctgtatggag
     1321 accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct ggactcctgt
     1381 taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg atgtaaaggt
     1441 atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa tgactacatt
     1501 caagtggctg atttggattg acatatctat cactcctatt ttataaactg attagtgaat
     1561 agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa agctgtataa
     1621 ttgcgccaaa ctttttcttc catcaatccc caattctcag gatcatccgt ataatttttt
     1681 gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc atatttttgc
     1741 aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt tttcgtggca
     1801 tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt cgtaataatt
     1861 accggagtat agtaatctaa ccgttcatct aattctttca atgggatata gtttagttca
     1921 atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg gatatctgag
     1981 gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag ttttgaaaag
     2041 tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg actttgccaa
     2101 cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc tgatagggtg
     2161 gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg tatattctgt
     2221 ccatctgcgt gagcataagt gacatcttct tgataactta caccaaattg tgctttgtcc
     2281 gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg gacatttaaa
     2341 ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg attagtattc
     2401 ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttatttcttg tttgctactt
     2461 gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa aagccataat
     2521 tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac ccttttttca
     2581 ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg atcgcagcaa
     2641 aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct aagcctgctt
     2701 gagcgcttag ccattctcca acgactgctc cgctgacaca ataagtagca gccactttta
     2761 aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa atcttccctg
     2821 tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc ctcaatgtat
     2881 ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc acttttggaa
     2941 gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt ggcaacattt
     3001 gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa tccattgcca
     3061 aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct tcatttaatg
     3121 tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa aatacagcag
     3181 caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc tgccaaataa
     3241 ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg ttacctcctt
     3301 tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa cccgcttcaa
     3361 gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg gtctaagttc
     3421 acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt ttttaaagtg
     3481 tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat ttcaataaat
     3541 aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac tcttttttag
     3601 tcaatttttg attgatgacg agtgtcaaat catcgatcaa ttcgtctgct tgctcgctaa
     3661 tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc ttagcaactg
     3721 gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc acttgatctg
     3781 acgaagaggc gtgacttttt gtaactccta cttcagcaag tttaggactg gcgtagacga
     3841 tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc cgcttagcta
     3901 cataatttcc ttcaaaagtg gcgactggtg tcaattttgg ggttttcctt gaaacaatat
     3961 ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact gcaattccgt
     4021 gtttatcaaa gacgatattc gcttgctcaa gtgctaaaga ttctgtattc ggctgtctcc
     4081 cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact aaacgatagc
     4141 gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga atgccagatg
     4201 cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt aatggccgtt
     4261 gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta gctagctcaa
     4321 aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc aatgataaaa
     4381 agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta ggtctttgcc
     4441 cggttgccaa aactaagtaa tccgcatgga agacttcttc gttgacttca atagatgaat
     4501 catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct aattgttttt
     4561 ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt tcttcccagt
     4621 tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga ttacgtgcct
     4681 ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa gtcccgcccc
     4741 atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca taagcagcac
     4801 ttaagccgct cacacctgaa ccgatgatga ttgcatcata tgttttcatt tccttcctcc
     4861 tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact tctttgcttt
     4921 tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt cataggcgta
     4981 tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata tccattgacg
     5041 actaaatctt ctactcgcca ctctttcgtc tgtttcggtt catcaaagaa atgccgaacg
     5101 tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat aatttctcca
     5161 tccttcataa atgcaatacg atcacctagc ttgattgctt cttccatatt gtgcgtaacg
     5221 aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg taatgagttt
     5281 cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc tgggcttgca
     5341 gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc tctaggcata
     5401 cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc tacacggtct
     5461 gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt ttgcttgact
     5521 gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca ccgcattttc
     5581 tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc tgatggttta
     5641 gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg accaaccaaa
     5701 acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata ctttccatca
     5761 tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc tcctttacta
     5821 atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt tcgacattga
     5881 cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat ttattcagtg
     5941 ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt gcgccttgat
     6001 aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc ttgagctggc
     6061 tatctgtgga ataggcatcc acaatattga catctccatt attgattgct tggtaacgca
     6121 aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct tggattcctt
     6181 tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc tgatcagaga
     6241 tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat ctcttgactg
     6301 ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg aattgcttat
     6361 tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca cttttcattg
     6421 cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga tagatatcga
     6481 tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc ggttttaatt
     6541 caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc aggatttcag
     6601 gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag gcagactgat
     6661 ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg gaatatcgag
     6721 gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc aaggcagaac
     6781 tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac aaaataaatg
     6841 tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg attaaaacta
     6901 aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct actttcagca
     6961 agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac gggtcaattt
     7021 cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata acaagtgcaa
     7081 tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat aaggcaagtg
     7141 aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt tttttatgat
     7201 tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa gaaatgagag
     7261 aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca cttaatgttt
     7321 gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa catttctttc
     7381 ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa aaagaaaaaa
     7441 atacctctaa atatgctgac aaaaaaagaa tccaagcaac aaagtcggat caactttttg
     7501 gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca cttggaatca
     7561 gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc caataaagta
     7621 gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat actgtatcat
     7681 gacacttttt tgccaaaaat gtaaaaaatg gttgttttga catcataaac gtcttgtttt
     7741 cttattttta cttatatcac cactttattt tctatctctt tcactccata aagaggaatg
     7801 agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa aaaaagaaag
     7861 atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa ttgatttgct
     7921 ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa aaaaactcct
     7981 tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc tgctcgcatt
     8041 tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt tttcattttc
     8101 ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt ttcaaaagaa
     8161 aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat cattttaggc
     8221 gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct ggcagatggt
     8281 ggggttactg gtattgcttt gcttttgcat aatctttttg gattcgctcc ttctgtcact
     8341 tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg aaaacacgct
     8401 tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt atgggagagc
     8461 cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac aggtattctt
     8521 tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg aacagatatc
     8581 atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct tttcttgatt
     8641 acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt tctgtataca
     8701 ttacttagct gttctattct atcttataca ttgaataaag tgaagtatct ggaaattgga
     8761 tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc acttgatcct
     8821 cttgaacagt tagagcactg attcttattt ttcactactt tatttaattt acagaaatat
     8881 cattatgtaa actaaaatga tattattcag taaaatcttc tttataaaaa aataaactat
     8941 ttttgcaaac gatctgcgaa aatagtttgt ttttttattt tgccattatt ttcttactta
     9001 ttgggagaaa aaattcgctt aaactcaaaa aaatttgagg atacgatata aactttcgga
     9061 tagagtataa aataaggaaa cgccgcagcg tttcctttcc aactgtagcc gaatataaat
     9121 ttgtatatca cttatttaaa atagctattt tctcccaaag agtttccttg atcatcgatc
     9181 aatcctcgta atgagcgttc taagatacca tggctttttt tcgatttttt ttcttctgac
     9241 tgctttgttt cctctaacgt ttttgttcga ttgaatgttc gtacagacgg ctcaccattt
     9301 cttttttctt gatacggcgt cggttctgta tcgaacaaca gataagagtc atccgttttt
     9361 ttcatcgatt cgattaaatg atcctcagaa attttgcttt ctctagggtc tgggataatc
     9421 gatgcgggta catatttagg aacaaaataa gaacgctccc gcaccttcgt gtcaacagat
     9481 gcgtttcgtg tagtatgctt ggactctgtc cttgtttcac tttgtctgac agaacgagag
     9541 gaatcaccaa aaagtttttt tcgtcctgtc gatgtgattt ctgattggta atggccggta
     9601 taatccggta attttccttt atgcttttcc aaattttcct tacgcggagc tgacttcaat
     9661 ccatttgttg cacgacgctt gcgacggatt ttctcggtgt tcgtgttcgt gaaatgaaat
     9721 tctttcatct gttcaaacgg ggactgattc tgttcagtca aaagatcttc ttgtccgtca
     9781 aataatactc gcttcttgtc gttggttaat tttacgccta actcatcatc gtacgcagga
     9841 aaacggaaat gttttcgtct gatttctttc attcaaacca tccttctcaa aaatggaact
     9901 tttctcaata atagcacaga ttcattgttt tcagtagcaa tgcgctgatt attttaagaa
     9961 aatagcgatg aatttcataa atatatatat agtaataaga cttaattcgt tgtctctctg
    10021 ataactagtt caggaaaaaa acgacgataa ccttgactcg cccctttttc ttctattttt
    10081 tttaacaaca tttctccaca tacttcaccc atacgtataa tatcttgctt gatcgttgtc
    10141 aatttaggtg aactgatttg gtctaaaaat accccatcaa atccaatcac accaaattct
    10201 tcgggcaagt gtcctccagc atttataatc cctcttactg ctcctagtgc tagccgatct
    10261 gaactacata caaaacaagt tttatctgta aaatctgccc agttttctgt catatatctt
    10321 tctacttctg tcgaatgatt tggaaaccgc aagattttac taggaagatc aacttccttc
    10381 atcacagata aataaccagt ttcccttgaa atttcaaaag gctcgtccac ttcgataccg
    10441 atataaataa tcgtttgata atctctttct aaggcatatt gcgttgcttt tgctgtacca
    10501 tattcattat cgctgtctac ataatcatat ccttcagaat tttccccaaa caagacaact
    10561 ggtttttcca aacggtcgat ccaatcgaag tctttttttc tgacacctgt gatgatgtat
    10621 ccatcacaag aaccgacgtc gaaggcatca tttgtaacta attggagaga ataatgcgct
    10681 ttaccgatcc gtttagcgat tcccatcagc aaattcatgt aatagggctc agttgtgtcg
    10741 atttcttcca gtataaataa tttgatgatc tgcgaccgat tcgtgacaag tgcttttgca
    10801 gcaatatttg gacggtaatc cagttcagcc attgctgaga aaactaactc tttcaattcg
    10861 tcagtcacct gttccggatg attgatgact cgagagaccg tcatttttga aacattggca
    10921 cgttttgcaa catctgctaa agtgggcatg ctctcttcct ttctaacatg ttgtcattct
    10981 tactatcaat tataccaaat tttcctaaca gagtttatta tagcccaaaa taaaaaacgg
    11041 aaaatcctct atcgtcatga gtgattttcc gtttcaatta tttatacttt tttatcaaat
    11101 tttttgctaa aaaagtggaa gcttcttcca ttagtggtat ttgtaccata tgtcgctgtg
    11161 tttttgagga acgaaaaaca atttcttttc cgtaagactt atgccgattt ttttctacaa
    11221 atcgttccgt ttgtatataa gggatttttt cgtcagcttg tccatgccaa aagaacagag
    11281 gacgtccagc taacttttcc ggctgcaatg acaaatcata gctcttaacc catgaaatca
    11341 aatcaaaata gtcttttggt acgacataat tatattccga tgcgcgcctt acgatttctt
    11401 ttccatattc caaaggtgca ggggaaccca tcaaacacac gcccgctact atatgaggat
    11461 gtttagccaa caaggcgcat gttgtcattc cccccatcga aaccccgcca actgcaagct
    11521 gatcatgtag aagacctctt ttttctaaat gcttgataag tgtgtcaaat tcaaacagat
    11581 ttccgtagat actatcccaa aatgtatagg atggaataga agaaaccggc tgtttgcgct
    11641 ccccatgatt catcgcatct ggcaggatca cacgaatgcc ctgctgcgca atttttcttc
    11701 cttgcgtcaa aactaactcc ttacttgtct gccatccgtg ataatagatg acaacgggaa
    11761 tacgttcctc tcttaattct ttcagaacaa cttctaacac tggaatatga tggacagaac
    11821 gtttcaatat aattggattc aattgttact cacctatttt cattaaattt cctgatcgcc
    11881 atagcaacca tgctccaaca gttgtctgga acaggttcag tgtaccaaat aatataaatg
    11941 caggactgaa tggctcggga aataagcaca gagaacaaag tcccaccact cctaatgcta
    12001 gcgaaaggag tggataaata gaaaaattca aaaaactggc aacgctaccg aaaatattac
    12061 caataaacag tatcaaaaag aaaaaaacgc ttaaaattaa accatctgtc agcgacatct
    12121 gaaaaacaat gttctctacc tcaattggct gttgaatcga atagacacct aagtatatac
    12181 tgagaaggag acttagcacg tggctggcac aaaacacacg attgaccgtt ttcattttaa
    12241 tcacctaacc tatctttata attctattat aatcatttta tcattttttt gtatgtataa
    12301 agtttcaatt tttcttcact tacgattttt tttgatattg ttgttatata aatgaaagga
    12361 gttttacaaa tgattgatcc aaaaatgttg gggatgatct tcctcattaa ttttgcttat
    12421 atcactctga acacgttacg tttcatgttg acaatgaaag gctatcgact gattgctcca
    12481 cttgtcagta tggtcgagat cacgatttat atcgtcggct tatcccttgt tttagatcgt
    12541 ttagataatc cactgaattt atttgtctat gcgttaggtt acgcagtagg tatcagtgtc
    12601 ggaattttga ttgaagacaa attggcatta ggatacatca tggtcacaac gattttgcca
    12661 gcaaatagcg cagaagataa aagtttgccg ggtatactga gagaacacgg ttatggtgtg
    12721 acacagactt ctgcaatggg gctggaaggt gagcggctag ttctcgaaat actttctcct
    12781 agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa tcgaagagcg tgcttttatc
    12841 atttcttatg agccaaaata tatttcaggt ggtttctgga ctaaaaaagt gagaaaacga
    12901 caaggaaaat aactcacaaa cttaaaaact gtctaatatc taaattaaat tttaaatgtt
    12961 agacagtttt tatttattat tttgattaaa aaacatcgac atatactcat caatttgtta
    13021 ttatacttta tatacttatc aatttaattt ggagatatta tggaaattca acgtagaacc
    13081 actaattttt tacatttagg ctcttttttt gcaactttga cgatcttatt ttttctatgt
    13141 tattcatacc ttaagacctc acatgcgcct tctagtgcat taggctggtt gcttttggat
    13201 cattctgata tcacagaagc cgaagaaaat ccgttttttt tgattttgtc ttctctttgc
    13261 ggcttactgt tttctgttta tttcggtgcc tatttttttc atcgacatcc tggtacgtta
    13321 aacgaagctt caagcgtatt gattgcattt aaaacgtttt cttttttagc aattcttttt
    13381 tattcgttta atcacaatac gcttttcttc tttatcatga atggtttagt cgtgatttta
    13441 tccattttta cttattttat tttttcactc atttttagcc aattgaaatg tccgctcttc
    13501 tttcgattag ttcctgtcac ctgttttatt tacagcttgt tttccttttt tgtccttgct
    13561 ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag gaaatctgtt ggatatgctc
    13621 ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa acaaacaaaa taaaaagcat
    13681 tttgaagcct gactattacg acttcataaa tgctttttta gttttaaaca cttcaatcta
    13741 aaagaataac ccaagattgg ataaattatt aatgactcgc cattcgtttc ttagtgattc
    13801 tggattatca aatggacttc ctaatctaat cagctagttc ttcccataaa atgccatact
    13861 tgactgaatc ataatattga ttatttaaaa atctaacttt tcttatcact acttcctttg
    13921 tcattccaca tttctcacca tttttttgca ttcctttgtt tccagaccat gtagtgaatc
    13981 taataggtgg tagataggca aaatactcaa ataattcttt taaccataaa ggaagagaaa
    14041 ggctcctcct gcaagagtca gtataatttt tgcttactgc tctcttaggt tttactcact
    14101 aaaactgttc catattcttt gatataagaa aatgcatgcg gtataaattt cttgctcagc
    14161 tcagaaaaga tttgtcgttt aagaaaggcc atttcctcca ttgtcaatga aggatccaat
    14221 gttggtaatt caatctcaaa attgtttttt tcgacaatag gtaattctaa tgtgaatgat
    14281 tcttcttcct ttatagtatt cgtcacaagt agagactgat ggaaaatact aggccggcga
    14341 tttttatatt tattcttacg tatcctgtca acgataactt ctatttgatc aaaaaagtac
    14401 gattcattta cgtctccaat aagttcggct aattctttga atgccttcgt tacaggaagc
    14461 tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg ggaagtgttc tatagaggaa
    14521 agtgtgataa aacggtactt ctcatttcct gcagttgtcc tcagcaattc tgtctctcgg
    14581 ctgtttctta ctccataaaa atacgcttct ttccgttcat cttcgaaaca atacgcccaa
    14641 ataacttctc ccatcgctaa tttttgttca tcaacagaga gtctcttcaa ttgttcagct
    14701 atggtttcag cagccgaagt ggcttgttgc aagtaatctt taggatcaaa tattggaaag
    14761 ttcaaatgat atcttcgttc ttccctcttg attaatcctg catcgatcaa taaatcaaga
    14821 aaatgggaaa atttattttg cgaaaattct tttttcaatt ctcttagaat cgtttcttgt
    14881 tctcgatgtt catatagata atcggtcaat gaagaaaaaa gcggatcatc ggtcagtttt
    14941 tgataaaagt tttccttatt ttttgaataa tagttcatat atgttctctt ttctatccgt
    15001 tttattttac ttaaaaggct tttacgtaaa aaggaggaag aacaagagtc ataattgact
    15061 gttattctcc tccatttctt cggcctttag tatgttctat gtttcttttt atatcaatcg
    15121 cttatccatt cattttgaat gcttcatact accatttgat tagctaaata cagccgtcaa
    15181 ttgaggtacg acttgttttt tacgtgaaac gacaccttta agcaaagcac ggttgttggt
    15241 caaagttgtt tggaaagctt cttctacttt ttcttttcct ttaccagcaa caagtaattc
    15301 tgaatcactg tctaagatgt ttgtgacaac taatacgaac agatcgtagc catttgcttt
    15361 catttcatct tccattgctg cttctagctc tgcttgtcga tccatcactt cattaaggtc
    15421 cactgtattg acttgagcga tacgaacgtt ggaactgccc attgggaaac tttttgcatc
    15481 caaatcaaga agaaccgcag ctgatttatc acttagattt gttcctgctt tcaacatttc
    15541 aagtccataa gtgtttagat cgacttcagc gattgctgca agttcttttg cagctgcttc
    15601 atcttcaggc gtacatgttg gtgatttgaa caacaatgta tctgaaatga tagctgagag
    15661 catgattcct gcgatttctt taggaattgt tacttggttt tctttatata gttttaaaat
    15721 aatcgtactt gtacatccaa ctggttctgc acgatagtac aatggattag ctgtttcaaa
    15781 gtttgcaata cgatggtggt ctactacagc taaaatattt acttcgctga tatctgaaat
    15841 actttgttgg aattcattat gatccacaag catgacatct gcattttctg ctttagctga
    15901 ctctactact cgtggtgcag atagctgaaa gtggtctaat gcgtattgtg tttcttcatt
    15961 aggtgtacct agagcaactg cttctgtttc tttacctaat tcattttgca agtgtgaaaa
    16021 cgcaatggct gccccgattg catctgtatc tggattttgg tgaccaaaaa ctaaaatttt
    16081 tgacatgtca ttcgctcctt tgtgatttct cctatttaat aatagcaaaa aaacggaaag
    16141 aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt aaatattttg tatactcttc
    16201 tgtatgaagc aatcgtttag cattttcgac acgttctttt gtaggtggtt cgatcccttc
    16261 taatggatat ttcaaaccaa gtgtttccca cttgtatttt cccatcgtat gataaggaag
    16321 aatctctact ctgtctacat ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata
    16381 ttcatcataa tcacttcgct caggaactag tacatggcga atccaaaccg gtttgttgat
    16441 ttttgacaaa tattgagcca tttctaaaat attttcattg tcccaatgcg ttagtttttt
    16501 atgctcttca ttatcgatgt gtttgatgtc aaataacagt aaatctgtat acttcatcaa
    16561 ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa ggttttccgc atgtatccaa
    16621 tgtagtatgc atcttccgct cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg
    16681 caatagaggt tctccaccac taacagtaat accaccttta tctccccagt aaggtctgaa
    16741 gcgttctgct ttatctaaaa gttcatctgc tgtgtattct ttccctccgc caatattcca
    16801 agtatctgga ttgtgacaaa attggcaacg catgcggcat ccttgcataa acacgacaaa
    16861 gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata gaatgaacat agccgattgt
    16921 tttttcttcc atttaagttc acatcttttc tttgatttca ttttaagtac aagttagctg
    16981 tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt
    17041 tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact
    17101 gattattcga ttttgatgcg atggctattc aacgtccttt cgacgctaag tcgaaaaact
    17161 tttttcacga actttttaca ttctgtcgtg tgaagttctc atcaagacat ctaattgttg
    17221 ttctcttgtc aagtcacgga atttaactgc ataaccagat acccggatcg tcaagttcgg
    17281 atatttcatt ggattttcca ttgcatcacg caataaatca ttactgaata cattgacatt
    17341 caaatgataa gctccttttg agaagtatcc atctaagaca ttacgcaagt tttcgattct
    17401 tgtatcttgt tcttttccaa gacctgaagg attgattgtt tgggtatttg aaattccatc
    17461 caatgcacag ccgtaattca ttttagctgt tgagttcagg ctagctaata acccactttg
    17521 ttccgcattg taacttggat ttgctcctgg cgcaagtggt tttccagctt cacggccatc
    17581 aggagtattt cctgtatttt taccgtaaac aacatttgaa gtaatcgtca ataatgaagt
    17641 tgttggtttt gagttgcggt aagttttatg acgtttgatt tgtcccatga agtattccaa
    17701 gatccatgaa actaattcat ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc
    17761 atccacttca aaatcaactg ctattccttt ttcattacgg attgctttta cttttgcata
    17821 tttgatcgct gacaagctgt ctgccgcatg agagatccca gcaatacctg tagcaaatgt
    17881 gcgttgcaag tcagaatcca tcaaagccag ctgagctgct tcataagaat atttatcatg
    17941 catataatga atgatgttca acgtattgac atatagttct gctaaccaat ccatcatatt
    18001 catgaagtta tgtttcactt cttcaaagtc tagtatttct gatgtgattg gacgatattc
    18061 tggtccaact tgggcgccag tcacttcatc gactccgcca ttgatggcat ataatagtgt
    18121 tttagccaag ttagcacgag cgccaaagaa ttgcatgtct tttccgacaa ctgttcctga
    18181 tacacagcaa gcaatcgcac aatcatcaga accccattgt tcacgaagct gattatcatt
    18241 ttcgaattga atcgaagaac tttcgatagc gattctgcta gcatatgttc tgaatccttc
    18301 tggaaggtgt tcagaataca aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt
    18361 taatgtatga agcagacgga aatcattctt tgtaactaat gaacgaccat ctaatcccat
    18421 acctgcaatg gataaagtag cccagatagg gaatcctgag aataattggt tatattcagg
    18481 tgtacgcgcg aatttgacca tacgtaattt cattactaag tggtcgatca attcttgtgc
    18541 ttctttttct gtgattactc cattatccaa atcacgttgg atatagatat caaggaatgc
    18601 tgagatacgt ccgattgaca tcgcagcacc attttgtgat ttgattgctg ctaaatatcc
    18661 gaagtatagc cattggacag cttcttttgc atttgcagct ggtttagaaa tatcatagcc
    18721 atatttcgca gccatttctt tcatttgcgc taatgcacga tattgttcag tgatttcttc
    18781 acgtaaacga atcacttctt cactcattgt atgataacca gtcatatcat gatctttttt
    18841 cttttgttcc atcaaatagt cgattccata caatgccaca cgacgatagt caccgatgat
    18901 acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt ttgtttttac gagcagcacg
    18961 catttcagca gtataagcgt caaatacacc ttgattatgt gttttgcgcc agtcagtgaa
    19021 aatatgtgtc aattcatcgt ttggtgtata gccattgctt tttagcgcat tgttagccat
    19081 attgattccg ccgaacggca tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac
    19141 gattttttcc aaatctttat ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat
    19201 ttctgtgtcc atatcataga caccgttacg ctcgaattgg atatcattta acttttgcaa
    19261 ttcatcccat aatttttctg ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg
    19321 atattcagtg tagttatttt gaataaagtc tctagtgtct acgccttctt tccatttggt
    19381 acctttaaat ccattccatt gctccattgt atggacctcc taaatttctg ttttatgtaa
    19441 cagtttttac gtgattagta taacacagca ccctatcttt gcaagggttt tcttgttttt
    19501 tatttaacaa tttttatgtt ttatttacat atatttaaat caacgttgac ataccaacat
    19561 ttaagcattg tgaacacttt catctttttt tgcaaacgta taaggattta ttaaaaatga
    19621 atgaaaaaga gacgccacct gtattaatac agatggcgtc tcttcattaa aatactacaa
    19681 cagttacgac tcgatgaaaa tctcaggagc atcgataact tcgatgactt ctccaccttt
    19741 tttctcatcc atcgcaaatg atccattcga tgttcgatca cctataggga aatcagttgg
    19801 gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt atttcttttg atcttccttc
    19861 cgacatatag ataatacggt gcggattttt tttcagctca cgcatgaccg tcactccccg
    19921 cttcgcacgt gaagtttggc tgatttcttg agcaagcatg cgtttaacag ctcctcgctg
    19981 agtaacgatt acgatcggcg tatcaccttc tgctaacaca agcagcccgt tgataatcct
    20041 atctccatct ttcaaattca tagacttcac tccagctgcc tttgctccaa caacgggaac
    20101 atttgttaaa ggatagcgca aacccattcc ttgatgcgtt actaagaaaa catctaagcc
    20161 tggctgatct tctttcagat acacagcaat cagttcatct tcttccgcct ttagcttcat
    20221 acagctgagc ggacggctct tataggtgcg ccatggttcg aactctgtca ttcgtgtttg
    20281 cttgatcaat ccattcttgc tgatgaatac aaatgttttc tctgcttgaa ttttttgata
    20341 tgggaaaaca gctaaaatcg attcgtctac agacagattg gagattgttt gagaaatgtg
    20401 ctcaccagca tctttccatt tcaaatcggg tagttcatgg acagggcggt agatcacatt
    20461 tgctttattt gtgatcagaa gaacatggtc caatgtactc aattcacctg agtatagcaa
    20521 gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa gcactataag aacgaaggct
    20581 tgttcgcttc acatagcctt ctcgcgtcac actgacaatt acgtcttctt gtgccactaa
    20641 gacttgcgta tcaattttga tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga
    20701 agaataattt tttttcactt ctcgcaattc ttttttcata acagagaaaa gctctttgtc
    20761 atttgaaagg attttattca actctgtgat ttgcgcaatc aagctttcgg attccttttg
    20821 aagctctgtg atatccgtgt tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc
    20881 ttgttcttct gtaaattgaa agacgtcgac taaattcttt ttggcgtctt ttttgtcttt
    20941 actttcacga atcgttgcga ttacttcatc cagaatcgat aaagctttca tcaatccttc
    21001 tacgatatgc tgtctttttt gagctttggc taaatcgaat tgactacgtt ttgttatgac
    21061 ttgctttcga tgctcgatat aacttcttaa aatgtctttc agtccaactt gatgcggagt
    21121 catatggtcg atagcgacca tattgaaatt gtaattgatc tgtagctcag tgtttttgaa
    21181 caaataattc aaaattcctt gagcattggc gtcttttttc aattcgacga caatttgcag
    21241 acctgttcta tcactttcat ctcgtacttc agctattcca tcgattttct tattcaaacg
    21301 gatctcatcc atttttttca ccaaggtcgc tttatttact tcatatggga tttctgtgat
    21361 cacgatttgc tgtttattac ccttcattgc ctctatcttt gttttcgaac gaagaataac
    21421 ttttcctcgt cctgtttcat aagctttttt gatttcatct tttccttgca agatccctcc
    21481 tgttggaaag tcaggtccag gaatatattc catcaatttt tcaaggctcg cttgtggatg
    21541 atcgatcata tagattgtgc catcgatcac ttcagctaag ttatgagtcg ggatttctgt
    21601 tgcatatcca gcagatatac ctgtcgatcc attgacgaga aggtttgggt atcgagctgg
    21661 aaggactgtt ggttcttttt ctgtatcatc aaaattccac acgagatcga ctgtttcttt
    21721 ctcgatatct gctagcatct ctccacttaa cttggataat ctggcttcag tataacgcat
    21781 cgcagcaggt ggatcaccgt ccatactccc gttattcccg tgcatttcga tcagtacttc
    21841 ccgcaacttc cagtcttggc tcatgcggac catcgcttca taaatactga tgtcaccatg
    21901 aggatgataa ttccccatga tatttccgac agattttgct gatttacgga atcctttgtc
    21961 atacgtattg ccatctttgt tcatggcaaa taatatccgg cgttgcactg gcttcaatcc
    22021 atcacggata tcaggcagtg cacgttcttg gataatatat ttggaataac gtccgaagcg
    22081 gtcacccatc acttcttcta aagttaattc ttgtacttct tgtcgatttt ccatcaggtg
    22141 acctcctatt ctacttcaaa taactgattg ttttcatttt tatctgctcg ttcttcatct
    22201 aataaatcat tagaaactga aggagagatt tcagtatcct cttttttatc taagatactg
    22261 ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc attttcttcg aggttctact
    22321 ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg cagcatcatc gatacggacg
    22381 cggatcaacg tacgggaagt cggatccatc gttgtttccc acaattgttc cgcattcatc
    22441 tctccaagtc ctttatatcg ctgcagcata tatcctttgc cgactttttt aatcattgct
    22501 gccaattcgt catctgtcca cgcatattca atgacttgct tctttccttg tcctttggat
    22561 actttatata aaggcggtaa cgcgatatat actttcccag cttcaatcag cggtttcata
    22621 taacgataaa agaaagtaag cagtaatact tgaatatggg cgccgtctgt atccgcatcg
    22681 gtcatgatga ttactttgtc gtaattacag tcttcgatag aaaactcagg accgactcct
    22741 gcaccaatcg tatagatcat cgtattgatt tcttcatttt tcaagatatc ttgcatttta
    22801 gctttttcgg tattgatgac ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct
    22861 cttccttgtt tagcagatcc gccagcagaa tccccttcga ctaaatacaa ttcatttttc
    22921 ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa gaagagattc gcctttttta
    22981 cgcttcttcc catttcggct ttcttctctg gctttacgtg ctgcttctct cgcttctctg
    23041 gctttgattg ctttacggac caacatctgg ctcatttcgc tattttcctg caaataaaag
    23101 cccatttgtt cactgatgac gttatctact acggtacgag caactggcgt accaagtttt
    23161 tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta cacggataga aagtacagca
    23221 gctaaccctt ctctgaaatc actaccttcc agattcttgt cccgttcttt cagcaaacca
    23281 acttttctcg catattcatt ataagatttc gtcatcgccg ctttcatacc agcttcatgg
    23341 gttcccccat cttttgtccg aacgttattg acaaaagata aaacattttc tgaataaccg
    23401 tcattgtatt gataagcgac ctctacttcg ataccttctt tttctcctga aaaataaaca
    23461 actggtgtca atgtatcttt ttcttcattc agataatcaa caaattcttt gattccttct
    23521 tcgtaatgaa aaatttcctt gactggttct tctccacgaa gatcagaaag agagatttta
    23581 acgcctttta gaagaaaggc agattctctt aaccgttcgg ctaaaatttc atatgaaaat
    23641 ttcgttgtac tgaatattgt atcatctggt aaaaaatgga cagtcgtccc gtttgctttt
    23701 tttgtttttc ctatcttttt caaagtaccg tcaggtttac cgccgttttt aaatttctgc
    23761 tgatactcta cgccatctct aacgatcgtt acaattagcc atttcgataa ggcatttacc
    23821 acactggctc ctacaccgtg cagtccacca gaagttttgt accctccttg accaaacttt
    23881 ccgccagcat gcagcacagt aaaaatcact tcaactgttg gtattccgga agcatgcatt
    23941 cctacaggca tcccacgtcc gctatctgca acagttatac tattgtcttc atggatggtc
    24001 acatcaatct cactgccata acctgataat gcttcgtcaa ctgcattatc gacgatttca
    24061 tagactaaat gatgcaagcc tcggctgtca gtggatccaa tgtacatccc tggtcttttt
    24121 ctaactgcct ctaatccttc caatacttgg atggacgagt cattgtattc gttgtttact
    24181 ttttttgcca aggcaaacgc tccttcaata atactttgcg gcaaaaccgc accacgatta
    24241 tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga gaaatcaatt ttaattatct
    24301 cctaaccgct ttttcgacat tcccctctat cgatcatctc ttctttgtgg tcatttttat
    24361 ttttggtttt ctttcaactt accaagttca atcttaatac aacgatccat tacgatatct
    24421 gtacgtcctg cttttcttag catctcagct gcttctttgt tttcaatccc taattgtgcc
    24481 cagaacactt tagcatcagt tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc
    24541 cgtctaaata catctacgat atcgattgga tcatcgattt ctgtcaaatt cgcatagact
    24601 ttctcgccta gaatttcttc accagccaat aatggattta ctggtatgat tttatatcca
    24661 ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct
    24721 acgacggcaa tattttttgc ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt
    24781 tttacagaca tttctgccac cccttgcttt tctttttatt ccatctatta tacttgtatc
    24841 tttcattttt tcaattgttc ctgttaccta ttcatgctaa aataaaggat agttttcaac
    24901 ttgaaagagg gtaaaaaatg aaattgatct tattacttat tttagcatat cttttaggtt
    24961 ctattccttc cggtgtctgg attggcaaat tgtttttcaa aaaagatatc cgacaacacg
    25021 ggagcggtaa tactggaacg accaatacat ttcgtgtatt aggcaaaaca gccgggattg
    25081 ttgtcctgct tatggatatt ttaaaaggta cattggctac ttgtttgccg atgatcttcc
    25141 acctttctgg gattaaccct ctttggtttg gggtatgcgc cattcttggc catacatttc
    25201 cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac aagtgcagga atgcttttag
    25261 gatttaatcc tgtatttttt gtttattctt gtatcatctt tatcgttagt ttattttgta
    25321 caagtatggt gagtttgagc agtatgatca gtgcagtatt gatcacttta tctacgatcg
    25381 tccttcctta tgtagctcct gtgattttgg ctaagcccaa ttggttactg acgatcattg
    25441 cctttttagt ttcttccttt atcttttatc ggcataaaga caatatcaag cgtatcagaa
    25501 accatactga aagtcgaatc ccgtttggtt tgaatagcag caagaaaaaa tcctgatact
    25561 ttttcattcc aaagagataa aacgggagtg tgacaaatcg atgtcacact cccttattac
    25621 ttacaggata gcttttttac acaccttggt aaacggtgtt ccctgctaac ttctcggtca
    25681 caacctctta cttcactgtg atggaatatt ttgtcttgaa gatttcatgg gcacctagtt
    25741 gattgatccc tactttttcc atcaagtttc cactactgtt caccgtgtca gcgattccgc
    25801 accacggttc gatacaaacg aaaggcgctt cttttggata aggagaccag atccctacat
    25861 aaggcatatc tttatagctt aaagttacgc tatgggaaga tttttcactt cgaatggcgt
    25921 acgcatttag accccttgtt tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca
    25981 aagccagatt cgtatttgtc tgacctaacg tcttttgatc catatcgatg aatgggcctt
    26041 tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg
    26101 acaatccttc ttcaagaggt acattgaaag caggatgtcc accgatagaa aagaacattt
    26161 ctttttcacc ggtatttgcc acttgataat gaaccgtgat gccgtctcct cctaattcat
    26221 agctgatcac taattcaaaa tcaaatggat acttttctaa tgtctccggc gtactcctta
    26281 agatgaaact agccatttct tgtccatgtt caatgacttc aaaatccata tcccgagcaa
    26341 atccgtgttg ccccatttga tatgttttcc cttgataaac atactgatca tcttttaaac
    26401 gtccaacaaa agggaataaa accggtgcat gtctgcccca atacgctgga tcaccttgcc
    26461 agatatactc taaaccatgt tcttttgatt tcagactgat caattccgca ccgtgttcag
    26521 cgatttcggc gatcagctgg tcattttgaa taataacagt cattgccatt cctccaactt
    26581 cgacttactt tttaaaatgg gaaggctgga acaaacattt ctatctgccc cagtcccctt
    26641 acttctaata actgtgtttt gctaactcgt ctttaaaacg ggaattcaat acttttaaat
    26701 aagttccctt catgcctaac gaacgagatt caatgattcc agctgattcc aattttcgta
    26761 aagcattgac gatcacagaa cgagtgatgc cgatactatc ggcaatatta gaagcagtca
    26821 gacgaccttc atctccatcc aatgcttcaa agatagcttg tacagccttc aattcgctat
    26881 aagataatgt gttgattgcc atttggacag ccgttgcact ccgtacatct gcttcgatgc
    26941 ttcgagactt ctgataaagg atctgcatac cgacaaccgt agctccatat tctgctaaga
    27001 ctagatcgtc atctgcaaac gattgttcca tacgagataa gataatcgta cccaaacgtt
    27061 cgccagctcc aaagatcgga acgacagtcg tcagaccaaa tggatatttc tctcttgact
    27121 ctaccggaaa tgcagtaata tcactagtaa ttgggatatt agcttcagtt ttcatcaact
    27181 gtgccactgt atcagtataa gattcaggaa attgtttctc aacaaacatt gttttgattc
    27241 gttcattgtt cacatcgtga cgttcattat aacctagtaa tactccgtca cttcctatga
    27301 tataggtgtt gctgtcaaga ataccaccta gtgtgacagc cattttatcg taaggcaaat
    27361 ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg
    27421 ttagcaagtc tgtcattgtc gtcctccgat tttttataaa atatagcgac ttaaatcttc
    27481 atttgccgca attgagccaa gtttgtcatc cacatatgct tctgtgatcg ttatttctcc
    27541 catttgcata tctggtgctt caaataatag atcttctagt agtttttcca aaatggtatg
    27601 cagtctgcgt gcgccgatat tatctgtgtc acggttgaca tcgaaagcga tttgagcgat
    27661 ccgttcaatt gcttcttttg taaacgtaac tgacacattt tctgtaccga tcaacgcgac
    27721 gtattgtttt actaaagcat tatttggttc ggtcagaatc ttcacaaaat cttcagctgt
    27781 caaatcatct aattctaccc gtatcgggaa gcgtccttga agttctggaa ttagatcact
    27841 tggtttagac aaatggaaag ctcctgaagc aatgaatagg acgtgatctg tttggatgat
    27901 cccgtatttt gtgtttactt gtgaaccttc gacaattggc aatatgtctc tttggacgcc
    27961 ttctctggat acttctccag attgctgtga tttagaagtg atcttatcga tttcatcgat
    28021 aaaaataatt ccactttctt ccgctaattt gattgcctca ctatggatat cagcatcctt
    28081 gactaatttt gctgattctt ccttgatcaa taactctaaa gcttctttga ctgttaccgt
    28141 ccgttccact tttttcttcg gtgtcaaagc acctagagtt tcatttaggt cgatccccat
    28201 ttgctccaat ccattgttca tcattggtgc aggtttcttt tggtcttcaa cttcaatggt
    28261 tacttcacga ctatccagta aacccttttg tagttgttcc aagactgtac ggcgattgat
    28321 tcgaatatct tctgtcactt cttcttgcgg ttcagcggta ttttgctgac tagcattaaa
    28381 aagattcatc atttgttcaa attgattagt ggattgtttc tgttcttttt tgataccagg
    28441 taccaagatt tttaccaaac gtttttctgc atttttttta gcacttgtgt atacacggct
    28501 gtactgctct ttttctacga tttggatcgc attttctact aaatcccgaa ccattgattc
    28561 tacatctctt ccgacataac cgacttcagt aaattttgtt gcttcgactt tgacaaatgg
    28621 tgcattgacg atttttgcca aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc
    28681 gatcatcagc atattttttg gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg
    28741 gcgatagcgg ttacgcaaag caacggcaac agattttttt gctgcgtgct gtcctacgat
    28801 atattcatct aattcactta cgatttgtct tggtgtttgt tttaattcat acatatctac
    28861 tcacctctta cagttcttct acgataatat tatgattagt aaagatacag atatctgcgg
    28921 caatatttaa cgcattttct gcaatttctt tcgcactcat gtcgttttga gcatagtttt
    28981 tcattgctcg ggctgcagcc aacgcaaaat tccctcctga tccgatagct aagataccat
    29041 catctggtgt gataacttct cctgtacctg aaacaagaag catttcttta tcattcatta
    29101 cgatcagcat cgcttcaagt ttttgcatag attgctgtgt ccgccattct tgagctaatt
    29161 cgacagctgc gcgttgtaga ttcccgttat attcgtttag tttgccttca aatttttctt
    29221 ctaaagtaaa cgcatctgca acacttccgg cgaaacctac aacaacttct ccattgtaga
    29281 tacgtcgtac tttctttgca gtgcctttca tgacaacaga ttcacccatt gttacttggc
    29341 catctccagc catagcaaat ttcccgtctt tttcgacggc acagatagtg gttgaatgaa
    29401 atggtgattc gaccatgttt gtttcctcct aaatttgttc ttttccttat ttttacgctc
    29461 ttggatggaa agaacaatag tttttttgta aactttcttt tgttacatgg gcatagatct
    29521 gggtagtcga taaattggca tgtcctagca attcttggac tgtccgcaaa tctgccccat
    29581 tatttaataa atgagtagca aatgtatggc gcagcatgtg aggatggata tcactatcca
    29641 aactgctttt tttgatcatt tgattcaaga catattcaat tccagtaggc gtgattggtt
    29701 ttccatgatg attcacaaaa acgatatcat gctcttcgtg atattttgtc atcaatacct
    29761 ggcggccttc cctgaaatat tcttgcagag catcttgagc aaaagaacct agaggagcat
    29821 atctctcttt gttcccttta ccatggatca acatgacctc tgaatcaaaa tcaatgtctg
    29881 ataccgacaa actgctacac tcacttaaac ggatgcctga cccgtaaagg acttccagta
    29941 aagcgcggtt cctcaaatcc aaaggactat ctcctgctac gctgtcaaaa agtgcttgca
    30001 tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag
    30061 aaaatggatt ttcttttatc acttcttctt tgagaagata ctgataaaac gaacgtaggc
    30121 tggctatctt gcgactgata gaatttctgc tatagctagg acgatgatca gctaatgcac
    30181 tcaaatagac acgaaccgta aaatattccg ccttcagcag atcagcgtct ccagattcct
    30241 ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc ttctttcgtt ttttctgaat
    30301 atcctctttc gacaatcaaa taacgcaaaa atttttgttc ccacgtggct tctgtcattt
    30361 tctcaccttc cttttgtccc agagaataat ttagcacagc gtttataaaa agacaaataa
    30421 attgtcagaa tttaaccaat atttctaagt ttgttcacaa ttggcacaat tcctcacatt
    30481 ttgtttgaga aattcttaag aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct
    30541 cttcatggag tttccttact catttgggat tgcagttttt tctaattctg tttttattgc
    30601 ctcattagct tttaaagctc gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat
    30661 gcgctctggc agttcaggaa acagaccata gttggcattc atcggttgga aatgttttcc
    30721 ttccgcatga gtaatgtagt aagccatact tccaatagct gtttcttgtg gaaactcgac
    30781 tggttccatt tcttttgcta agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc
    30841 actttcaaca tagccttcta caccagtcat ctgacctgca aagaacagat cttcgcgttt
    30901 cgttgattgg taagtcggtt tcagcaattc tggcgaattc ataaagctgt ttcggtgcat
    30961 cacaccatag cgaacgaatt cagcattttc taacccagga atcatccgga aaacacgttt
    31021 ttgctcgccc catttcaagt gagtctgaaa gcccacgatg ttgtataaag aagctgcagc
    31081 attgtcttga cgcagctgga taacagcata aggacgtttg ccagttttag gatcttccaa
    31141 tccaactggt ttcataggtc cgaaaagcat cgttttgaat cctcgctgtg ccattacttc
    31201 aattggcata catccttcaa aatatttttc tttctcaaaa gatttaagtt ctgccacttc
    31261 ggcattgatt agtgcctcat ggaaagcttt gaattcctct tctgtcatcg gacagttcaa
    31321 gtaagctgct tctcctttgt catagcggga cttcaaatat actttatcca tatcgatcgt
    31381 agctttgtct acgattggtg cagctgcatc atagaaataa aaaccatctg aaccattgaa
    31441 tgctttgatg ctttctgcta atggatgaga agtcaaaggt cctgttgcta ctacggtgat
    31501 tccttccggt atttcagtaa tttcttcgtt tatcactgtt actaatggat gctctttgac
    31561 ttttcgggta attgtttcag aaaaagaatc gcggtcaaca gctaaggcac caccagctgg
    31621 cactgctgtt tcatctgccg aagtgattac taccgaattc aatcggcgca tttcttcttt
    31681 caataaccct actgcattag ccaaactgtt tcctcttaat gaattggaac atactagttc
    31741 agcaaaatta cttgtgtgat gcgcctcagt cgatttgact ggacgcattt catataaacg
    31801 gacagggact cctgcttgtg cgatctgcca agcagcttca cttccggcaa gccctgctcc
    31861 aacaacattt accattttca atgaaagaac ctcttttcta ttatttttgg atgtcttctt
    31921 cgtaatctcc atttacacag actacttgtt taccgccttt gactttcttc tcgaccagat
    31981 atccgtcaca ttttggacaa tttctgccca ctggtttatc ccaagaagtg aattcacatt
    32041 ctgggaaacg ggaacaacca tagaatagtc tgtttttctt agattttcgt tcaatgactt
    32101 gtccttcatg acatactggg caagtcacac cgatttcttt tacgattggt ttcgtattcc
    32161 gacaatcagg aaaattactg caagcataaa acttcccata tttccctaat tttatcacca
    32221 tcggatgtcc acagacatca caatcgaatc ctgctggttc atctttgatt tgaatttttt
    32281 cgattttttc ttccgcattc gtcagttctt tttcaaacgg tttatagaaa cgatcaacca
    32341 ctttgaccca cgcttccgtg ccttcttcaa ttttatccaa atctccttcc atttctgctg
    32401 tgaaatggat atcgacgatt tgagggaaga attcacagat taatgaattg acaatttctc
    32461 ctagttcagt cggctcaaat cttttttgtg tcaattttac atagtagcgt ctttggatcg
    32521 tatcaagtgt tggtgcataa gtggaaggtc gccctacccc gttttcttct agtgcacgaa
    32581 tcaacgtagc ctcactaaaa cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat
    32641 caacagattg gactacatcg ccttcttcaa gttctggaag aatattttct ttctcttctt
    32701 tgccgtcatc tctaccttcc acatacactt gcatgaatcc tttgaatttg atttttgaac
    32761 cgttcgcaat gaagatgaca ccattttgtt ctaatgtcac tttcattgta tctaaaatag
    32821 ctggtgtcat ctgactagcc acaaaacgtg accagatcaa tgtataaagt ttcaattgat
    32881 ctttatctaa atattgtttg agttcatctg gtgtacgaag cacactcgtt ggacgcacag
    32941 cttcatgggc atcttgtgcg ccttgagcat ttttgatctt tcggtgatca tgtctagaaa
    33001 attcttttcc atacgtatct tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa
    33061 tccgggtaga atcagtacgc atgtatgtaa tcaagccgac tgttccttgt ttacccaaag
    33121 tgattccttc atataattgc tgtgctacca tcatggtttt tcttgttcga aagtttaact
    33181 tacgggcagc ttcttgttgc aggctacttg tagtgaaagg attcgcaggg tttcttttgc
    33241 gctctttctt ttctactttt ttaacttcga agtctgttcc atcaattcta gcagtgactt
    33301 cttttacact ttgtgcatct ggtaatttct ttttcttacc gtcgattccc cagaaatttg
    33361 ctttgaattt cttgcgagct tttttaaaat taccgtcgat gctccaatat tcttctggaa
    33421 caaattctcg gatctctttt tcacggtcaa taataatttt taaagcgaca gattggacac
    33481 gtcctgcact taaccctttc ttgaccttac gccagagaat aggactgatc gaataaccaa
    33541 cgattctgtc taaggtacga cgagcttgct gtgcatctac taaatctaca tcgatcgttc
    33601 ttggttcctt aaaagctgct ttgactgcgt cttttgtgat ttcattgaag accacccgat
    33661 ttttttcttt cagatccaac ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt
    33721 ctctatccgg gtcacttgcc aagtaaactt tttgagcttt ttttgctgcg gctttcaggc
    33781 ttttgatgac atcgcctttt ccgcgtatag aaatataatg gggttcataa ttgttttcaa
    33841 aatcgatacc cattttactt ttcggtaaat ctcttatatg gccaacactg gccattactt
    33901 tatagtttcg tccaagatat ttctcaattg tcttggcttt tgcaggtgat tctacaatca
    33961 ctaaatattt atacgccatt actgaatgga ctcctttcca ttgtcgtgtt cctactatta
    34021 aaagaggatt catacaaatc gtagctaatc atatccattc agaaaacgtt tgtcaagaaa
    34081 agcaaatgaa aagtatggtg ttattcttta aattctgcca agatatcttc acccgaaacc
    34141 gtgcaaactg caccgtcttg gatcaaatga tgacatccgg tagaatgttc tgttaaaata
    34201 tttcctggaa cagaaaaaac tgttcttcca agatccaatg cttgctgtgc tgttatcaga
    34261 gagccgcttt ttttcttggc ttcaaagaca catgttcctt tagacaaagc tgcgataatc
    34321 cgatttctca ttggaaaaca atgctttttc gggccggatt gaggaggata ttcactcaaa
    34381 agaagatgat catttgcaat cacctcttgc aagtcacgat tttcagcagg atagacttgc
    34441 tctatccctg ttccaatgac tgcgattgtc cgtccaccat gagagattgc tgcttggtga
    34501 ccgtatgtat ctatcccttt cgcgcagcca ctgacgatca catatccagc ttctatcact
    34561 ggttttagta aataataaac tgtttttttg gctaaagcag aagtgtgtct tccaccaaca
    34621 acgcttaaca tgttacaaga aagtaaagat agatcaccat tataaaacag tacaattggc
    34681 ggataggtca agtgaagcag ctgaggggga tatttgggat caaagcaagt gataaacgac
    34741 tgtttcgatt ggattttgcc ccagttagcg tgaatttcct gccaactttt gcgaaacagc
    34801 tcccggtgtt tggttatttt acctatctta atgatgtctt caaatccgat ttctttataa
    34861 ggatactgca tcatccagtt agcaagtgcc cattttcctt gactactgat tccttttgta
    34921 taagcaagtt tcaataaatt ttcttctatt tgatacatca taaaaccgcc tttcgtctct
    34981 ctttctaata agatacgtaa aagcggctgt tttttattgt aaatattttt ttacaggtgc
    35041 aaaactttta cgatggattg gcgtaattcc ttgtcgttca agtccttcta agtgttcctt
    35101 tgttccataa cctgcattat gcgagaaacc atatccagga taaagcacgt catattcttt
    35161 catcaattgg tctcgatata ctttagcaat gatacttgca gcagcaatcg acacagatcg
    35221 tgcatcccct ttgattattt tttcctgctg aataggcaaa tccaaggtca tcgcatcgat
    35281 caacaaatag catggtacaa tggacaattt ttctactgct tggatcatcg ctaatttact
    35341 tgcttcataa atattcacat catcgattgt ctgctgatca acgatcccga tcccgattgc
    35401 cagggctttt tcttgaactt gttgataaag aagttctcgc tttttctctg ataattgttt
    35461 ggagtcattc agccctaata tgatctcatt tttcggcaag ataactgcag ctgccacgac
    35521 aggtccagct aatggtcctc ttcctacttc atcaatgccg gcaatccatt ggtgaccttg
    35581 tgcgtaccct tgcttttcaa agcaaaacat ttcttcatat ttttccttaa ttaatgcttc
    35641 ctttttcaat cgtcgttcaa aagaagccag tgcttgctgc acgcctttgc gtggatcatt
    35701 tcttaatgat tctagtgtag gatcatctat tctctgtatg cttttcaatc gttctttgat
    35761 actttggatg gattcagtca tcactttttt gctccatttc ttcgcaacga tccagtgtgt
    35821 atcgtccaag ttttccttga cgaatatcat gcacgactaa ttcgctgcca cgatcatagt
    35881 cttctttata cccacgtttt gctgtaatat ccatcagtaa ttccggtgcc aatttttctt
    35941 cttgctcgat tgataaacca taacgatcgc tgattcttcc tgaataatat cgggcaaaaa
    36001 agtctaatcc gtaaatcgct aaatcatcta aatggacaag ctggtctttg atcgcgcctg
    36061 tcagcgctag ttttttgccg atttcttcat cttcaaattt gggccataaa atccctggtg
    36121 tgtctaacaa ttccaactca ttacctaaac gaagccactg ctgacctttt gttacgccag
    36181 gcttatttcc tgtctgtgca attttcttgc ctactagtcg attcatcaac gtagatttcc
    36241 caacatttgg aatacctaag accattgccc gaatagcgcg aggcttgatc ccccgagcag
    36301 catctcttgc ccgtttttct ttcaatgctt ctttagccgc aggtactaga cctttaattc
    36361 ctttactttc ttgtgccgtg atagctaatg caagatggcc ttgtttacga aaatattctt
    36421 gccacagctt tgtctgttgt ggatctgcca gatccgcttt attcaataaa acaaggcgag
    36481 gtttttgctg aatgatctga tctagcattg gattacgaga agataaaggt aatcgtgcgt
    36541 ctaccaattc aaatacgatg tccacatatt tcattttttc agaaacttct cgtcgggctt
    36601 ttgccatatg tcccggaaac cattggatgg tcattgttct ctccattcct ttcataccgg
    36661 aatattcccc ggctttactt gtttttttct ttttcaatat aaattcactt ttttattata
    36721 aagaaattcc atctattagt taagtgaaag tgaaccagtc ctcattctat cacatcttca
    36781 cttttttgat gatgcaaatt tttattttgg tttctatata tgaatctaaa tttttgacag
    36841 cgtttcctaa ataagagatg atagacgaac aaaacagaaa ggagacgaaa caaatgaata
    36901 gtatggtttt tgttaatttt cctgttgctg atgtcaatcg ttccgttgct ttttatgaaa
    36961 agctgggttt taaaaaaaat gaagaatttt ctactgaaga agctggttca atggtatggg
    37021 atgacacttt ttggataatg cttctctctc atgattttta taaaaaattt ataaaagata
    37081 aagaaatagc tgataataaa aaaacaagtg gtgctttgat ttcgtttagt gttgaaaatg
    37141 cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa tggtggtact ttccacgaag
    37201 tagatatggg aatgcctgaa gaagaaatgt tttcattaga agtccaagat ctagacggaa
    37261 atacattaga acccgtatgg atgaagatgt aagcagataa ctaatctgac gttttttata
    37321 acaaaaaaac aaaggcggat gatacgataa gtattctccg cctctatttt ttttattcgt
    37381 acaatctctt ttttacagtt tataaaagca gtcttctcac ccttttttta attgttagat
    37441 gccagtgtat gtaaatcacc aatgtgtcat gacacattgg tgattttatg gtatcctttt
    37501 attattaact gacaaaggag agaatatttt gaacactttg attgcaaaag acgtttttag
    37561 attgcccgaa atgaatgcaa gattgttgtc gacctctgct cttttattag cccttgatgt
    37621 catcttatac aagatagcga ttggtcctgc ttattttcaa atcacattcg gatttttaag
    37681 tatggcgctt atgggttatc tatatggacc gatctgggct ggtttgcttg aaatgttgtc
    37741 gaacacttta aatttcacga tatttggttc aggaacgttt caagctgctt ttcttatcac
    37801 ggctttcctt ggtggattta ttaatggctt atttttatat cataaaaaga taaacctgcc
    37861 ttccgtcatc gctgcccaat tattgattat gatcccagtc agccttttta tcaattcttg
    37921 gctgatgtct gttttgtttg gtgcagattt caaagtaatt tttctaggta gattcatcag
    37981 gaatatgatc caaatcccta ttcaaataat ggttgtttat tcatttatca aaactttaga
    38041 ggctagaaaa gtgtttaaac gagctgtttg attttacgta ctaaggtagt aaactgattt
    38101 tggattaaac aataaagcgt gcagtctcaa cagactgcac gctttattgt ttctcactgg
    38161 atccatttta tttcatcaag tggataatag acaaatcttg ctttacctag aatatcagtt
    38221 ttattgatcg tgccaaacga acgactatct tttgaaactc ttcgattatc tccaagtaca
    38281 aaataattat cttccggaag cttggattgt cccgttagct cttgtagtgt aaagtccgtc
    38341 gtataggatg catttgcatg atctgatttc aggtttttag ttagataagg ttccttaact
    38401 actttcccat tgatcttcaa ttgatcattt tggtagctga cattttctcc tggaagaccg
    38461 attactcgtt tgatataaat cgtgccatct gctaattgaa aaacaacaat atcaaatcgt
    38521 ctgatctccg aaaatttttc catcacaacc atatcacctt gcttcaacac gttttccatt
    38581 gaattgcctt ccactggtac cgggatgaat aaaaatcccc gcaacatgaa cgcaaggaat
    38641 acagaaacaa gtaagtattt gaatacaagc cagaagcgat taatatagcg ttgttttttt
    38701 gtcaatctct atcaatccat ccctcttttt ttcattcttt ctttattata agaaaaatat
    38761 caaaaaaatg cgactagata gctgaaataa aaataattct ttcaattagg ctattggaca
    38821 aaaaactttt ttgatcataa agtacaaaac tatctcttat atgtcatgat tattttctaa
    38881 atatttttcc catgcttcgt caaagatatc catggacggc aaatagtcga cattcagttc
    38941 cagataatta gaaatttctt cgtagctaga gctgtgctta ggaaattgga tgtctttacc
    39001 ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca ccgcctcgca gtgtcattaa
    39061 ataatgaaaa aagcttcgat tcacccttcg ttcctccaat tttcttcgat aaaggcagcc
    39121 cggcgtgcat ggtttctttt aaaattttca ggatgctttt tataatactc ttgatggtct
    39181 tcttctgcta gataaaatgg ttgggctggt tcgatttttg taacgattgg ctgttcgaaa
    39241 cgtccacttt cagctaactg tttcttactt ttttcagcaa tgatccgctg ttcttctgtc
    39301 aaataaaaga tcaccgggcg ataattgtca ccgcgatcct cgaattgtcc gaaagcgtca
    39361 gttggatcag tttgctgcca gtaaagttct actaattgtt catatgaaat caatgttgga
    39421 tcatagataa tctccactgc ttctgtatgt ccagtcgttt ggctttttac ttgttcataa
    39481 gtcggatctg gtacatgccc tccagtatat cctgacatca ctgtatggat accaggaagc
    39541 gtgtcgaaag gctggatcat acaccagaag cagcctcctg caaatattgc tcgttccatt
    39601 atgattcccc ctctaatact actcatccga tgatttttta ttttcttttg tcttgctttc
    39661 agtactgctg gtgctagttt tgttggtatt tgagttgttt gaagcatcag aggtatcttt
    39721 tggcaaatat agactaaacc gaatgtcatc atttactaag tcgattttat ctgctttaat
    39781 atacatcccg ttttgcattc ggaattgatc caacctaagc atgatagtca gatcatctgt
    39841 attgacttcg atccaatctg gtaatttata gtttctcttg atcatattca tcacatcttt
    39901 gattggcaaa ctcaatgttc cgatagaaag acttctagct tttagttgga catttccgtt
    39961 ttccatcaca tacggatcaa aatataaata gaaatcgata ggaaaaccta gtacttcgaa
    40021 agtaccattg agtagtgctt cattttccaa ataaaattta tattcaatat cactatcttt
    40081 ttgatactca ctaaggaaaa agctgatgat ttgattgact ttttctttat ttgaattgat
    40141 cgttaagaca ggatctcctt gtctttccgt aagagctggt atttctggga gttctggttc
    40201 ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa ccaagtacta ccccaagaag
    40261 gataaggaag gcgaatttcc agtaatttgt tttctttgtt ttgtttgaag atacgtcaga
    40321 tcttgttttc atgtttattg atcatcctcc ttctctaacc attggttttg tgtctcgatc
    40381 agctgatctc tcactgcatt tgccatcaat tgataaccta aattatttgg atggaaatga
    40441 tcttcttcat agagcacatt atttttgatg ccatttagat tttctgtcgt gctagttgtt
    40501 tgatctgttc cttcttctgt gacacctact tcctgattca atccttgata aagcaaatca
    40561 ttgattggga taaagtagca gttcggcatt ttttttgtca ccgtttctgt tcctttgttc
    40621 caattgttaa caattgtctg catgtcagtg atctcaggaa agttcaaata aaatggattg
    40681 tagatgccta aaatgtaaat aggtgcggtt ttattgaggg atcgaaattc ttccaatagt
    40741 tctgtgacat tttcctgata gtttttcagt ggctttttaa aggaattgac cgttaacccg
    40801 aataaattat tttggataac tttcatcaaa tcattaccgc caacagtcag tgtgataaaa
    40861 tcagctgttt tgatattgtt tcgaatttct tcttttgttt ttactcgctt taagatctgg
    40921 tcacttcgtt caccatttac accatagttt tctgtttcaa ccgcggtcaa attataacgg
    40981 tcttttagat catttgcgac aaggggaacg aatcctcctt gcttggtttg atctcctact
    41041 ccttcagtca gcgaatctcc gatagctatg tagtgaagtt tttctttttt atttggatct
    41101 tctctcacag tagttactgg tttcattagt gggccagctt tcggtataga agcagctaaa
    41161 cctgcataga tgatgatcgc aattaataat gggaacaaaa cgaggatcaa atgtcgtttc
    41221 gttaatttca ttttttcacc tcttgtattc gattggttct aatgttatca aaaattttat
    41281 caaaaaggta gtctttatga aaaaagaaaa caattatttt cctaatttta taaagaaaaa
    41341 gagaataagc tgcaagcctt tcaggctgag ggcttatcct cttttcgact aatctacata
    41401 atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg ataaccggat tcgtatgcag
    41461 tacagggata tgcatgtccg ggaataattc ttgcaaaccc tctttgaatt cttgcgccaa
    41521 ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca tttggcaatt ctttcagttc
    41581 atttttcaaa ttattaaacc atttatcgaa cgttttcttt cctcgcccct tagttacagg
    41641 gattaattca gaatgggcga aattcatcac cacgcgtatg ttcaaaacac tagacagcaa
    41701 accagttgtt cggctgattc ttccaccttt taccaagtta tctagtgtgg atattccgat
    41761 gtataatttt gtattgtctt gtactttttt gacttcttca aggattttct ctactgtttt
    41821 accttctttt gctaattcag ctgctcgaat tacctgaaaa gataatcctt ggtcagtggt
    41881 atcactgtcg ataaccgtta ctttcgtttt tgttagatta ctggcttgtc ttgctgcttc
    41941 tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc acttcactgc cgtcttctcc
    42001 tagctcatca tacatcgaga tgaattctcc aattggtggt tggcttgttt tgggcagatt
    42061 ttttgctttc gccatcattt ccataaatct ttcgccgtct agttggtcat catcgggata
    42121 tactgttccg tcgatcatga tggatagcgg catgacacga atgcctaaag catcgcgtac
    42181 atttttttcc atcgtacatg aagaatctgt tacgattttt acatttgtca ttattttctc
    42241 tctctctttc tttaacaaga tgccttattc atgttaaact acatgtaaga caatttctat
    42301 ttttagtata acagacaaat aatgattttt cattaattac acctagtaaa ggagccgttt
    42361 tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt aaatgaagtg tttaatgcag
    42421 tcacccatgg aattgggact ggattgagta tcgcgggact agtggttctt ttagtaaaag
    42481 gtgcccactt gcattcctcc ctccatattg tctcttatgc tatctatgga tcgatgatga
    42541 ttcttttatt tttaacgtct actttgtttc attcattgat ttttacaaaa gcaaaaaagg
    42601 tgttccaagt attcgatcat agttcgattt ttcttttgat tgcaggaagt tatactcctt
    42661 tttgtttgct gagtatccga ggatggctag gttggacttt atttgtatta atctggttgt
    42721 ttgcgattct tggcattata tataaagcgt tgacgttgca taaacgggag agagtatcaa
    42781 aagtatctac gatcatctat atttttatgg ggtggctctg tgtcgtggct gctgttcctc
    42841 tttatcactc tcttggaatg actggtgtta ttttgatggt agcaggcggg ctttcttaca
    42901 cgataggcgc attcttttat agcttgaatt ctatccgcta tatgcatgtt gtttggcatt
    42961 tgtttgttat gttaggtgca ggttttatgt acttctctgt tttattcttt acttaataaa
    43021 aaaaataaag ctatggatgg gacagctcta aataacagct agtctatcca tagctttttt
    43081 atatcgtcaa ttcgcctgtc ccgcttttta caggattctc attacgctga tacgtttcaa
    43141 attgatgcgg ataacgattt ttttcatcca tttctccttg actagtattg atcagtgacc
    43201 aatcagacca gtcaacaggt ggaaaataag catctccatc gaatacatga tggatgactg
    43261 tacggtataa tatcgtacaa aatggcagaa attcttgata aacagctgac cctccagcga
    43321 tgaaaaagat accgtctgtt tctttttcca gagcaagcac gtcttccaca ctatgcatga
    43381 catgcacgcc ttctgcttta taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg
    43441 gcaacggacg gctacccatc ccttcgaatg tttttcgtcc catcactagt gtatttgctt
    43501 ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt
    43561 ttccaatcaa tccgttttca tcctgtgccc aaattgcagc taacattttc tcatcctttc
    43621 tgattctaaa cagcgatagg agctttgatc gccggatgtg ggtcatatcc ttctaattgg
    43681 atatcttcca tctcaaaatc aaatactgac tgcttttcct gattcaaaac taaagtcgga
    43741 aatgcgcgta tttctcggct caattgttct ttcatttgtt cgatatgatt gatataaaga
    43801 tgagcatctc ctaatgtgtg aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt
    43861 aggtgagtca gcaacgcata acttgcaatg ttgaacggaa cgcctagaaa cacatcccca
    43921 ctgcgttgat acaattggca gcttagccgt ccctcttgaa cataaaattg aaacatcgta
    43981 tgacaaggag gcaaagccat gctaggaaca tcttccggat tccatgcaga aacgatcaat
    44041 cttctagaat caggagtctt tttgattgct tcgatcactt gtttgatttg atcgataaag
    44101 ccaccgtctt ttgtttccca gtgacgccat tgatagccgt agatgtgtcc aagatcgccg
    44161 tatgtttcag caaaggaatc atcggttagg atcttctcgc aaaaaagctt gctctgcttt
    44221 ttatattctt cattgaattc aggatctgtc aaacatctat ggccaaaatc agtcatatct
    44281 ggtccattat agtctggact tttgatgtag cgttcaaatg cccattcatc ccaaatatgg
    44341 tttttatttt gtaatagata gcggatattc gtatctcctt ttaaaaacca aagcaattcg
    44401 cttttgatca atccaaaagg tactcgtttc gttgttaaca aaggaaaccc tttttgcaga
    44461 tcaaaccgca tttggtatcc gaataagctt aatgtgcctg tgcctgttct gtcttcttta
    44521 cgatgccctt cttccaacaa ttttcttcct aagtcaagat acgcttgttc catccttttt
    44581 cctcctaatc ttctacccat tcactcaagt attcccaccg tgccattttt tcttcaagtt
    44641 gtgcttctgt ttcagatacg tcgttttgca attcttgtaa tcgagtgaaa ttatctcctt
    44701 gatgattcat ctcttcttgg agcaatgaga ttttttcttc aagttctgca atctcatctt
    44761 caatcgtttc ccattctttt tgttccatgt aagataattt cttcttttca gcaggttcct
    44821 tgctctgtat ttttggtttc gctttttcag caggctgctg ggtctctttt tgcttcagta
    44881 aataactgga catgtttccg taataaagtt cttgttttcc gtttccttgg aatactaata
    44941 acttatctgc taccttgtcc aagaaataac ggtcatggga aacagtgatc acagcacctg
    45001 gaaaacttct aaaataatct tccaaaatcg tcaatgttgc tatgtccaaa tcattggtcg
    45061 gttcatccaa taataaaaca ttaggttgct gaatcagcag tttcaacaaa aataaccggc
    45121 gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg cataaatctt ggaaataaaa
    45181 atcgttcaag taattctgct actccgattt gactgccatc tgctgttttt gcttcttcag
    45241 cagcttcttg taagtaagca atcatccgtt tatctggtgc catttcttca ttttcttgtg
    45301 tataatacgc aagatgaacc gtttcaccga tagacattgt accgctatcc aggggaattc
    45361 tgccagctag aatattaagc aatgtcgatt ttcccgcacc atttttacca gtaataccta
    45421 gtctttcccg agattgaatc aataaatcta aatgttctag cagtgtttgg tcatttatac
    45481 ggtaactgcc atctttgatt tccagtactt ttttccctaa acgttgtgtc gctaggttca
    45541 gctctaaatc atcttcttgg tttacttggt acaagttctc ctttagatct tcgaaacggt
    45601 tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc agccctcatc caagctaatt
    45661 cctgtttata aagccgcttg cgcttttctt cttgttctac ttctacacga tcacgttctg
    45721 ctttttctaa gacgtaagct tcataatttc ctttatactc gtataatttt ccaaatgaaa
    45781 gttcgaagat tcggtttgct actctatcta gaaaataacg atcgtgggta accatcaata
    45841 gtgcgccacg gtattgtttc aaatagtttt ccaaccactc gatcgcttca taatctaaat
    45901 ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc aatcaacact tgcgccagac
    45961 taactcgttt tttctgacca cctgataaag tactgatttt tttatctaat tcactgattc
    46021 ctaatttttg caaaataatc tttgcgttgg tatctgtagt ccaagcatct tctttattca
    46081 tttgttcttc tgctttagca taacgttttt gcacatcagg gtcttctccg ttttccccta
    46141 gttcgatcaa tgcttcctca tatgctcgaa cagtttggat caaggggctg tttccttgaa
    46201 agactgcttg gagaacagtt aattcatcgg aaaattcagc tgcttgagaa agataaccaa
    46261 tacggtaatc agttggataa aacactgttt gccgatcacc gtcgctgcta tcgataccgg
    46321 caataatatt taagagactt gttttacccg tcccgttcgt cccaatcaac ccgatacggt
    46381 ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc tttttcacca taagtttttt
    46441 tgaggtcagt tacttttaat tctttcaaca tgttccctcc ttatcttgga tattgtatca
    46501 taaaaatagt gaacttcagg aaaatttttt tggtatttgt tctttttgtg ttctcaaaaa
    46561 aagaacagga ccgattcgat cctgttctga tatgttttgt tttttataaa acttgaaaat
    46621 ttttaccttt ttattttatt ggactcgttt tttttgactt tgctcgaaac gcaaatcgga
    46681 aagaaaagtt atcagatggt ttgttcatcg tataagattc taacaaattg atattcctgc
    46741 gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac ttcgtattca tcaatgattt
    46801 ttctttcaag ataataacct cttagcattt ttttcgtaaa cagactatct tgctggatca
    46861 tccacgaaga aatcaaattc cccgagttca tttttattcc ttcaagtttg cgttgctgga
    46921 taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa gttatctaaa ctttcaataa
    46981 tcaactgagt atttttttga tatgtctctc tgatttcttg gatatcttca tcactaataa
    47041 atgtctgacg atcagcatat cttctttgcc aaaacatttt cggatggaga acgattcgca
    47101 ttaacctacg gacaacaatg aaccaaaagc cgataaaaga caaaatctgg cttgtgatag
    47161 aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata accattagaa gaaagcttgc
    47221 ccgaacgata actttcatcc aatccatccc gttcgatcga caaaatcagt gcttgtattt
    47281 cttgtacttc ttgcttttca tcatcggtta aatcttccaa gtacagatca cgtatccgtt
    47341 cttgataatt ttctatcact gcttcagtgg ctaaaaattc ttttgtatta cgatcaattt
    47401 cctttatttc ttttcgcaaa acttcaacga cctcttctaa aatcatcaaa gcattcaaat
    47461 ccgtgctttt cgcttcctct gtttccgtca gcataggcaa gacaatgatc cctatgacca
    47521 aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa agaccgctgg taaaacatca
    47581 tattattgat tgaaaatggc aaaataaaaa tcgtagccaa actgactgtt ccctttacac
    47641 cgccaaatgt taagatcaag atctcgttca actgtttttt gaacttttta ctgccatctt
    47701 tgaatacata aaaaagactg atagaaatga atctgatcac aaaaagcatg acagagatta
    47761 atacgataat cgctaataaa agaccgtttg gatataaacc gtctccccat actggcgaga
    47821 acacttgcgt caattcgatt cctaaaaata taaatactaa tgcattcagt gtaaaagcaa
    47881 tagttgtcca tgtactgtgg gacaagctcg acaattctgc atcaaacaca gagattttcc
    47941 taaaaccaga agcttggaga atacctgctg ccactgcagc aatgattcca gatacatcaa
    48001 atgcctctgc caatacatag gctagaaaag gaagcaaaag ttcgatcagc agataccctg
    48061 tgacatcttg tgctgatgct ttttcgatca aatgaattat tttttgtttg aaccagacaa
    48121 gcaaaaagcc gatcaaagct cccccaatac tagaaactac tagactcatc cccgcgttta
    48181 ctgctgaaaa agatccggtg atcagcgcac caagagcaaa ctggaaggca gtaacaccag
    48241 atgcatcatt caaaagacct tctccttcca aaatatgcat cgccttttta gggatattca
    48301 ctcgtccaga aagagaactg actgctaccg catctgtcgg acctaacgct gctccaaaag
    48361 caaagcaggc agcaagtggg acacttggca acaggaatga taacgtcgca cctaccccga
    48421 caagggtcaa aatgactcct ccaaatgcca agaacaaaat tgtcccgaaa tttttgagaa
    48481 tcgaagaaat atctgctttt tctccttctc taaataacaa tggtgcaata atcataacta
    48541 agaacaattc aggttcaaaa tcgattgatt gtccccactc tgtcagacct aataccactc
    48601 caaggaaaat ttggatcagt ggagctggaa tcattggcaa aatgcggcta gctatgttcg
    48661 ataaggtgat cgttactgca aatacaataa ctaactcagc aaattccatc gtccatcccc
    48721 tttatttttt attttgtgta agcattttta aaaattgttc ttttttcttt aaaattttgg
    48781 tttcgactac ttcaacctgt gtttggttcc cctctaattc acactgtaca cgttttgact
    48841 ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg atacatcaat atatttcttt
    48901 tacgcaactt acaaagtatt tgacggcggt catccatcga aagggcgcca tagcgatcat
    48961 gcaacagttt tttttgttgt tcaaaactct ctctcatttt ccagatctcc tatacacaaa
    49021 cttccatcat tatagcaaag ttctttttgg atgaaaaata tttcattgtt tcatcgttta
    49081 ttgctagtta gaaatttcca taaatttttc aacgcctcat tctattccaa atagtatcag
    49141 atatcaagta aaacttaaga aagataaaag tttctgacaa aaggatgttt actttcccat
    49201 tcctgccttt cttgttcttt tatcacaaca aaaaaatggg gctgcgacaa aaatcttgtc
    49261 acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag taataagaca
    49321 catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa ttccccgtat
    49381 acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt tttttcgctg
    49441 atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag atatgggaag
    49501 tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat atcttcgtat
    49561 ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc atgtgtcgca
    49621 actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg gatttcaaca
    49681 aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca aagatcgccg
    49741 atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt tagcgctgtc
    49801 atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta ctacctcagt
    49861 ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg acgtatctgc
    49921 ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta aatcaatttt
    49981 tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg ttctacgacg
    50041 gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt tccagtttct
    50101 tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg taaacgatca
    50161 taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat caattcttct
    50221 acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa agctccagtt
    50281 tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg gttagaacac
    50341 ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg gctgatcaat
    50401 aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc aaaacgtaaa
    50461 agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt ttcaataaat
    50521 gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc cacatttata
    50581 cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta gaaggtttct
    50641 tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat tcgtaaggca
    50701 tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc ttctagatct
    50761 tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa cgcattgatc
    50821 gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac atgatctggt
    50881 ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata ttgttcatca
    50941 ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt tggaaaaagc
    51001 tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat tggctggttc
    51061 agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc attctctaat
    51121 tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa tttcattttt
    51181 ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg gtgactgttt
    51241 gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta ctcgtcctta
    51301 cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc aaaaacttca
    51361 gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac atcaccaagt
    51421 tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc ataagtgtgg
    51481 ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg agctaatgga
    51541 gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg catactgtat
    51601 aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa ctttaacgca
    51661 aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag gaaggaattt
    51721 ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg gtcaaacgaa
    51781 aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca tttggtcttg
    51841 tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc accttgatct
    51901 taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac atcccattga
    51961 ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt ctaggtacac
    52021 tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc aatttacagc
    52081 atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga agcgggatcg
    52141 tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt ttagaacaaa
    52201 attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc ttacttgctt
    52261 cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg tatgttttca
    52321 gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta cttgtcgtct
    52381 ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga ggtgtgactt
    52441 tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat atggtcgtta
    52501 gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa aaagctttcc
    52561 tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg aagccacaga
    52621 caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg aaagattcca
    52681 catccagctg acctttgtct tttttagtac tatactattt ttaactaatt gttatgttca
    52741 cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac ttcatcagat
    52801 aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg agcataaagc
    52861 atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa ttcagtatct
    52921 tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat agatagatat
    52981 tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt tttccaattc
    53041 aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg caaaaacaat
    53101 ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc cgcaaaattc
    53161 gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag ataattcaag
    53221 atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt taaaaaattc
    53281 ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca tctaataatg
    53341 acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat tcttgcgctc
    53401 ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa tctggttcat
    53461 gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag tcttctaatt
    53521 cattataagc atgggctagg ttccattctg cataaggatt agcagtttct tccatctgat
    53581 tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta cttaaagtaa
    53641 gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt tcttctgctc
    53701 gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt tcgacttggt
    53761 aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc atttcttctt
    53821 cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta acttctcgaa
    53881 gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc gtaaaagcta
    53941 gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa ggtacgatag
    54001 ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc atcgagattc
    54061 ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct gcctctttga
    54121 aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt tcatcaggaa
    54181 gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg ataacttggt
    54241 acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta tccacttttt
    54301 ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct gctaacggga
    54361 tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc tgttttgctt
    54421 cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata tctgctgggt
    54481 cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca tctgataaag
    54541 cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc actgacgatt
    54601 tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg gtatccgtct
    54661 attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa aaaaagaact
    54721 ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata gccaagcaag
    54781 tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg gagattccac
    54841 acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag atggccattt
    54901 tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt ttctgcctct
    54961 aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt cgttaattgc
    55021 tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca aatcattccg
    55081 caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc gctgattgtt
    55141 tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg atttggtagc
    55201 tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga tacagggatc
    55261 atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag atccatgatc
    55321 ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg tttgacgaaa
    55381 tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca aatataccag
    55441 tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa atcataatag
    55501 cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag ctgactgact
    55561 agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc aagttccata
    55621 cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta gaaaagataa
    55681 gagcaaagct cacctcataa cttgcaaata aataaaaatt aaataaaata catgtgtttt
    55741 ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt ctgctaggaa
    55801 ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact gtcttattta
    55861 acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc tggaatttcg
    55921 atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg aacttcaaag
    55981 ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg aatagttgaa
    56041 aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc tgaagcaact
    56101 ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac aagagatatg
    56161 atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa agaaatattg
    56221 attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc aaggttttta
    56281 ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg tctttttctt
    56341 ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg ataattcgga
    56401 tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa cgttcataag
    56461 aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt ttgacagcga
    56521 cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt ggattgattg
    56581 ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga ttctggttca
    56641 tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc actgcagaaa
    56701 caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct tgttcgaaat
    56761 cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg ataataattc
    56821 cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga atcccttctt
    56881 cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt gcacgcatca
    56941 cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca gctgtatcta
    57001 tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg tctcgggtcg
    57061 ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg atcaaagagg
    57121 atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg tcctcttctt
    57181 cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc cctagtccat
    57241 ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca tagatatcgt
    57301 ttctcatttc tggattatct actttattga ctgctaaaat cactggttta ttacttctgt
    57361 ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg ctgactacac
    57421 aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc tgatccataa
    57481 aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct cgacctaacc
    57541 attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg acaatcgaga
    57601 tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg cggccaacga
    57661 ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc tcacttagtt
    57721 taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt atttttattc
    57781 cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag ctgctttcat
    57841 ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt ttcttctact
    57901 tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt ttcttctggt
    57961 aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc ttctaaagct
    58021 ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt cactttcact
    58081 tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga aatttgagag
    58141 atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa gcttgtcaaa
    58201 cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat ttctgaccaa
    58261 ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg attgatagac
    58321 aacactttga catttacatc atcattgaca gccaatacat cacttggttt tgctacatga
    58381 ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc gacaaatgcg
    58441 ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg gatagttgaa
    58501 agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg cgataagatc
    58561 aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt gcctttatag
    58621 tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa accacgtaca
    58681 ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc gatgaccttg
    58741 ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa acggcgtttt
    58801 gaaagtagat agctgccgtt ttctttatct ttgccgatcg tactgataac gactaaatct
    58861 aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt tgataattct
    58921 tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac ttgtttgtct
    58981 tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg tacgcttttc
    59041 attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt cataacactt
    59101 gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa ttgattattc
    59161 aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt tcattttttt
    59221 tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt gctgcaacga
    59281 cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct tgcttcagcg
    59341 gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc gtcagtgttt
    59401 caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct gcacgctcac
    59461 ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct gtcccgatgt
    59521 ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca gcaactaatt
    59581 cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca tctggttgac
    59641 ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct tcttgttgcg
    59701 aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct gaaaaagata
    59761 cattgttcgt aatagataaa taagttactg cgcgatacat ggcaccggta tctgtataaa
    59821 tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat gaagcaggcc
    59881 cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga aaaaaagccg
    59941 caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc ataaagttgt
    60001 ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca cgctcagcca
    60061 cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca gctgatgaag
    60121 aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt gcctcacttg
    60181 aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct gtatcttgag
    60241 acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca gttgactcct
    60301 cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa gaagttgctg
    60361 ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct ggaatagcga
    60421 tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct ttcttttgtt
    60481 gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt tgttcccatg
    60541 gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc actgttttac
    60601 ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc taaaaaatca
    60661 atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa gttgttcagt
    60721 cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa tatcacaaca
    60781 attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt ttctgcggca
    60841 gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt ctttttcctg
    60901 acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca gctccatcca
    60961 ttttttttgc agctcatcaa agggagcttg ctctgtggca tattccaaat aaagctcaaa
    61021 actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt catccccact
    61081 ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac cgatttcttg
    61141 tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga tatcagattt
    61201 atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat tttttacaaa
    61261 ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt atccaactgt
    61321 aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg cacaataaat
    61381 gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt tttgctgcgt
    61441 tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct cttttggtgg
    61501 tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg tcgccgttag
    61561 tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt gatattctgg
    61621 acggaaatca actccccatt gagacacgca atgagcctca tctactacat ataacgcaat
    61681 attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg gactcaaaaa
    61741 caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt gcgaaagtaa
    61801 actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca cttgatcttc
    61861 cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat accccgtcaa
    61921 ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat cttctccatc
    61981 aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc caaaatattt
    62041 ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag atttgataca
    62101 aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag cgccactctc
    62161 gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga atccactcgt
    62221 atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact gttcctcttt
    62281 ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac atctgcttgg
    62341 tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa gcgtgccaaa
    62401 gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg ctttgttgca
    62461 gataaaccgc tgactcatca ttgttttttg ccagctgata aggaagcagc cctgttcgct
    62521 catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc tccgaaaaca
    62581 gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat tggctttcac
    62641 taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga agatagtctt
    62701 gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc cgccaactag
    62761 tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt tgatgttcgg
    62821 acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt tcgatccaac
    62881 cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat tctggaaaac
    62941 tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa gtcgttcgtt
    63001 ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat gaaaatagcg
    63061 ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa tgtcactact
    63121 atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg aagcgccaga
    63181 tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag aggctcatca
    63241 acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa aatgtccagt
    63301 tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa aacgtacact
    63361 aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg aaatcccgac
    63421 ttattttgaa agattcattt gctatctata gttaattgac tagttttttc acggacccta
    63481 taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat tgacgatcac
    63541 agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct caccatagct
    63601 gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt ccggtaaata
    63661 ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg ccgtgatcga
    63721 taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac ttgccatgaa
    63781 gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac ttgccattac
    63841 gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt ctttgacctt
    63901 atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa tagcgactga
    63961 aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct ctacgagagc
    64021 accaggataa aaaataagta atggagactt tccatgattc tttttctctc cttcaaaaaa
    64081 taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg acgatggttc
    64141 gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta aaatgatgac
    64201 taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct atctgagtga
    64261 cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa atgcaaactt
    64321 tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt tttgtatgtt
    64381 tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat tgctttatac
    64441 atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt atcagggaac
    64501 atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc gcctgataca
    64561 aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt tttcactaac
    64621 tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata agtcaaccca
    64681 tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa tgtaacaggg
    64741 attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact tgcaaagttc
    64801 atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc accagttgca
    64861 ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac ttctgaaaga
    64921 gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc gtggatactt
    64981 ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg ttttttcaat
    65041 gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg aacaacattc
    65101 aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa gtaagcttgc
    65161 aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa tccttgcgta
    65221 attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc ttcatctgta
    65281 ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa agctttgacg
    65341 gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc aactaaccag
    65401 ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc ccctgctgga
    65461 acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat ctctgaataa
    65521 ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact gatttccaat
    65581 actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc tgaatctgct
    65641 tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc ttgttcatga
    65701 tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa aaagggtcat
    65761 gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag cctgctccat
    65821 cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga atggagctat
    65881 tgacttatcc acgtgcttat cgatagccgt ttatcggttt cttcattctt atgatagacc
    65941 ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc acacagatgg
    66001 ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa acatcgaaac
    66061 gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag tttactaaac
    66121 cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca aattcaccca
    66181 taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga atcaagaatg
    66241 acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat aatgaactta
    66301 atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc ataaccactt
    66361 ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa gcaccagcta
    66421 aaaattcaga gacaaattca gactctttca tgccataagt gattgctact acgatggaca
    66481 ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga agcatgcttt
    66541 tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat gattcaaaaa
    66601 tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg ataccaaaag
    66661 taataaagaa caatacatta acatgttttt gttttttcgt gattacttct tgacgaccga
    66721 ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact aatgattttg
    66781 ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa atagctaaca
    66841 aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc ccaacagctt
    66901 gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct acttgagacc
    66961 ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc atgactggga
    67021 tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag ccgcctaatg
    67081 caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat ttcttcacaa
    67141 ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa aatccaccaa
    67201 caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt agctcccccg
    67261 ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc ccttgaggat
    67321 tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct acactatagt
    67381 gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata aataagaccg
    67441 tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc ttcttccttt
    67501 cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa gagtatcaga
    67561 acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt tttccctatt
    67621 ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg tttccttttt
    67681 tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg caaagaattg
    67741 attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa cgtttacatt
    67801 attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg atctttttga
    67861 tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac ttaaccatgg
    67921 aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt tgatcaagtt
    67981 gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca tctgtgttgc
    68041 gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg cgatactcat
    68101 aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc ttcctttatg
    68161 ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg ttgataagaa
    68221 actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca gatgtaatac
    68281 tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca tgctgatcaa
    68341 taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc
    68401 aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca ttttctgtac
    68461 acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa ctttttgacg
    68521 tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag ttatttgatc
    68581 aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt atcggaattt
    68641 caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt
    68701 tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca ttatacatat
    68761 ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt tgagtggttc
    68821 agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg taggccttgt
    68881 aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc aactgcttga
    68941 agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac gatactgtgg
    69001 tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt tcgtccttcg
    69061 atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc ttttaccttt
    69121 gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca
    69181 tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac aggatagata
    69241 cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc atctgcaaca
    69301 gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc ttgataaatt
    69361 gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa ttcccttaca
    69421 acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt tcgacgagaa
    69481 gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat tctcctttat
    69541 tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt tccatttctt
    69601 gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag tagccagttg
    69661 ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca atcagtttat
    69721 gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag
    69781 ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg gcttgagata
    69841 atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct tttttagtcg
    69901 ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt
    69961 aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga tttaacagtc
    70021 cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg gcttcaagtt
    70081 cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat tgattcatag
    70141 ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa agccataaaa
    70201 gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc
    70261 gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg
    70321 ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat tttctgcatg
    70381 gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc agcatccgct
    70441 aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc tgctttttct
    70501 gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt gaccaattct
    70561 tctcgaggat cttcttccca taattcttca tcgtctgtga cgaattcttg gacaggaagc
    70621 aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc tcctgcaaca
    70681 gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt gatcgtagcg
    70741 atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag cagatcgaga
    70801 ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac tctttcttct
    70861 ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca cggatggaga
    70921 catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc tcaccgcgat
    70981 aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc cagtaaatac
    71041 caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt
    71101 cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc agtttcttat
    71161 ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt gttaacatct
    71221 tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc gtcattcttt
    71281 tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct aacaattcct
    71341 gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg
    71401 tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc agatttttcc
    71461 agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc atttcagatg
    71521 cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat
    71581 aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt accagtccaa
    71641 gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc atcccagtag
    71701 catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg
    71761 cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc tgtgctttct
    71821 tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc cgtaaaaact
    71881 gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct tttttttctt
    71941 catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc catgaagcaa
    72001 ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag ctttgtcgtg
    72061 tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg atttgctctt
    72121 tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt ctgatagtct
    72181 ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact ggttt
//
LOCUS       DACCPY010000002        70958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCPY010000002 DACCPY010000000
VERSION     DACCPY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.002"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            336..1439
                     /locus_tag="IVO69_000073"
     CDS             336..1439
                     /locus_tag="IVO69_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7398887.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IVO69_000074"
     CDS             1723..2403
                     /locus_tag="IVO69_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7398888.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IVO69_000075"
     CDS             2493..3527
                     /locus_tag="IVO69_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7398889.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IVO69_000076"
     CDS             3541..3876
                     /locus_tag="IVO69_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398890.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IVO69_000077"
     CDS             3860..6340
                     /locus_tag="IVO69_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398891.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IVO69_000078"
     CDS             6396..7955
                     /locus_tag="IVO69_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7398892.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IVO69_000079"
     CDS             7952..9316
                     /locus_tag="IVO69_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7398893.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IVO69_000080"
     CDS             9487..10557
                     /locus_tag="IVO69_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7398894.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IVO69_000081"
     CDS             10572..11240
                     /locus_tag="IVO69_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7398895.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IVO69_000082"
     CDS             11260..11838
                     /locus_tag="IVO69_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7398896.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IVO69_000083"
     CDS             12241..13152
                     /locus_tag="IVO69_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7398897.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IVO69_000084"
     CDS             complement(13408..14325)
                     /locus_tag="IVO69_000084"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7398898.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IVO69_000085"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IVO69_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7398899.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IVO69_000086"
     CDS             15022..15570
                     /locus_tag="IVO69_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7398900.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IVO69_000087"
     CDS             15685..18045
                     /locus_tag="IVO69_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7398901.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IVO69_000088"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IVO69_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7398902.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IVO69_000089"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IVO69_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7398903.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IVO69_000090"
     CDS             20551..21450
                     /locus_tag="IVO69_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7398904.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IVO69_000091"
     CDS             21443..22705
                     /locus_tag="IVO69_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7398905.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22866..24764
                     /locus_tag="IVO69_000092"
     CDS             22866..24764
                     /locus_tag="IVO69_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7398906.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25063..25782)
                     /locus_tag="IVO69_000093"
     CDS             complement(25063..25782)
                     /locus_tag="IVO69_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7398907.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26119..26451
                     /locus_tag="IVO69_000094"
     CDS             26119..26451
                     /locus_tag="IVO69_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398908.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26438..28441
                     /locus_tag="IVO69_000095"
     CDS             26438..28441
                     /locus_tag="IVO69_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7398909.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28460..28930
                     /locus_tag="IVO69_000096"
     CDS             28460..28930
                     /locus_tag="IVO69_000096"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7398910.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28951..29535
                     /locus_tag="IVO69_000097"
     CDS             28951..29535
                     /locus_tag="IVO69_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7398911.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANKLFE"
     gene            29550..30536
                     /locus_tag="IVO69_000098"
     CDS             29550..30536
                     /locus_tag="IVO69_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7398912.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30526..30837
                     /locus_tag="IVO69_000099"
     CDS             30526..30837
                     /locus_tag="IVO69_000099"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7398913.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30859..32640
                     /locus_tag="IVO69_000100"
     CDS             30859..32640
                     /locus_tag="IVO69_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7398914.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32633..34009
                     /locus_tag="IVO69_000101"
     CDS             32633..34009
                     /locus_tag="IVO69_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7398915.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34016..34651
                     /locus_tag="IVO69_000102"
     CDS             34016..34651
                     /locus_tag="IVO69_000102"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7398916.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34681..36036
                     /locus_tag="IVO69_000103"
     CDS             34681..36036
                     /locus_tag="IVO69_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7398917.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36228..37622)
                     /locus_tag="IVO69_000104"
     CDS             complement(36228..37622)
                     /locus_tag="IVO69_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7398918.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37714..38685
                     /locus_tag="IVO69_000105"
     CDS             37714..38685
                     /locus_tag="IVO69_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7398919.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38682..39463
                     /locus_tag="IVO69_000106"
                     /pseudo
     CDS             38682..39463
                     /locus_tag="IVO69_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(39632..40003)
                     /locus_tag="IVO69_000107"
     CDS             complement(39632..40003)
                     /locus_tag="IVO69_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7398920.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            40137..40580
                     /locus_tag="IVO69_000108"
     CDS             40137..40580
                     /locus_tag="IVO69_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7398921.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40623..41432)
                     /gene="yidA"
                     /locus_tag="IVO69_000109"
     CDS             complement(40623..41432)
                     /gene="yidA"
                     /locus_tag="IVO69_000109"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7398922.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41451..42818)
                     /locus_tag="IVO69_000110"
     CDS             complement(41451..42818)
                     /locus_tag="IVO69_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7398923.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42802..43632)
                     /locus_tag="IVO69_000111"
     CDS             complement(42802..43632)
                     /locus_tag="IVO69_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7398924.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43795..44232
                     /locus_tag="IVO69_000112"
     CDS             43795..44232
                     /locus_tag="IVO69_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7398925.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44308..44928
                     /locus_tag="IVO69_000113"
     CDS             44308..44928
                     /locus_tag="IVO69_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7398926.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45117..45623
                     /locus_tag="IVO69_000114"
     CDS             45117..45623
                     /locus_tag="IVO69_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7398927.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45640..46539
                     /locus_tag="IVO69_000115"
     CDS             45640..46539
                     /locus_tag="IVO69_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7398928.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46626..47511
                     /locus_tag="IVO69_000116"
                     /pseudo
     CDS             46626..47511
                     /locus_tag="IVO69_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(47703..47774)
                     /locus_tag="IVO69_000117"
     tRNA            complement(47703..47774)
                     /locus_tag="IVO69_000117"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47739..47741),aa:Arg,seq:ccg)
     gene            47834..49168
                     /gene="rpoN"
                     /locus_tag="IVO69_000118"
     CDS             47834..49168
                     /gene="rpoN"
                     /locus_tag="IVO69_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7398929.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49371..50408
                     /locus_tag="IVO69_000119"
     CDS             49371..50408
                     /locus_tag="IVO69_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7398930.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50449..51450
                     /gene="gap"
                     /locus_tag="IVO69_000120"
     CDS             50449..51450
                     /gene="gap"
                     /locus_tag="IVO69_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7398931.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51560..52750
                     /locus_tag="IVO69_000121"
     CDS             51560..52750
                     /locus_tag="IVO69_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7398932.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52854..53609
                     /locus_tag="IVO69_000122"
     CDS             52854..53609
                     /locus_tag="IVO69_000122"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7398933.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53725..55023
                     /gene="eno"
                     /locus_tag="IVO69_000123"
     CDS             53725..55023
                     /gene="eno"
                     /locus_tag="IVO69_000123"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7398934.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55422..56561
                     /locus_tag="IVO69_000124"
     CDS             55422..56561
                     /locus_tag="IVO69_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7398935.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56628..57203)
                     /locus_tag="IVO69_000125"
     CDS             complement(56628..57203)
                     /locus_tag="IVO69_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7398936.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57208..58182)
                     /locus_tag="IVO69_000126"
     CDS             complement(57208..58182)
                     /locus_tag="IVO69_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP7398937.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58206..58250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58465..59433
                     /locus_tag="IVO69_000127"
     CDS             58465..59433
                     /locus_tag="IVO69_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7398938.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            59530..59823
                     /locus_tag="IVO69_000128"
     CDS             59530..59823
                     /locus_tag="IVO69_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7398939.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59941..60177
                     /gene="secG"
                     /locus_tag="IVO69_000129"
     CDS             59941..60177
                     /gene="secG"
                     /locus_tag="IVO69_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7398940.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60289..61035
                     /locus_tag="IVO69_000130"
     CDS             60289..61035
                     /locus_tag="IVO69_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7398941.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61038..63395
                     /gene="rnr"
                     /locus_tag="IVO69_000131"
     CDS             61038..63395
                     /gene="rnr"
                     /locus_tag="IVO69_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7398942.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63417..63881
                     /gene="smpB"
                     /locus_tag="IVO69_000132"
     CDS             63417..63881
                     /gene="smpB"
                     /locus_tag="IVO69_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7398943.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64183..64902
                     /gene="atpB"
                     /locus_tag="IVO69_000133"
     CDS             64183..64902
                     /gene="atpB"
                     /locus_tag="IVO69_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7398944.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64955..65170
                     /gene="atpE"
                     /locus_tag="IVO69_000134"
     CDS             64955..65170
                     /gene="atpE"
                     /locus_tag="IVO69_000134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7398945.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65261..65785
                     /gene="atpF"
                     /locus_tag="IVO69_000135"
     CDS             65261..65785
                     /gene="atpF"
                     /locus_tag="IVO69_000135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7398946.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65772..66314
                     /locus_tag="IVO69_000136"
     CDS             65772..66314
                     /locus_tag="IVO69_000136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7398947.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66337..67893
                     /locus_tag="IVO69_000137"
     CDS             66337..67893
                     /locus_tag="IVO69_000137"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7398948.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67907..68809
                     /locus_tag="IVO69_000138"
     CDS             67907..68809
                     /locus_tag="IVO69_000138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7398949.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68832..70238
                     /gene="atpD"
                     /locus_tag="IVO69_000139"
     CDS             68832..70238
                     /gene="atpD"
                     /locus_tag="IVO69_000139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7398950.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            70252..70674
                     /locus_tag="IVO69_000140"
     CDS             70252..70674
                     /locus_tag="IVO69_000140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7398951.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23557 a        12441 c        15067 g        19893 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattgtttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 attcattgaa aatagagcat aacaacttta taatgttgag tataccaatt tttggaggcg
    22861 ttttaatgga ccgctattta gatttacttt cagagaagtt ccctacaacg gaagatgtca
    22921 tcacggaaat catcaatctc gaagcaattt tgcagcttcc aaaaggaaca gagttatttt
    22981 tgagcgatat acatggcgaa tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa
    23041 atcttaaaga aaagatcaaa gaccttttct ctgaacaaat gacggaaacg gagatcagcc
    23101 aattcactat ttttacggct tatccagaat atgtgttgac gactgattgg tacaaaaaac
    23161 aagataaatc ccagctgatc catcgtttga tcgatctttt acggtttacg acagtcaaat
    23221 acactcgatc aaaagttcga aaaggattgc cgaaagaata tagctatatc attgaggaac
    23281 tcttgtatat cgatgatcgt atcaacggga aaaaagacta tgtaaaaaaa atcattgaac
    23341 aacttgtctt gatgaaggaa gaagaaaaat ttttaacaaa acttgcgcag acgatccaaa
    23401 aatctgtcat cgatcatttg catatcgtgg gtgatatttt tgatcgagga actcaagcag
    23461 ccaaagtaat ggatcgaatc atggattttt cctctgtaga tatccagtgg gggaatcatg
    23521 atattttgtg gattggtgcg tattgcggct ctgaggcatg tttattgacg cttttacgga
    23581 ttgccgcacg ctacaactat ttatttgaat tagagaaaga atatggatta aatttacgtc
    23641 cattattttc ttttgcccat caaacctatc agaagaatcc agtatttacc cctaaaaaca
    23701 ggaaagatct atctgaaaga gagcaagaag aattagaaaa gatccatcaa gcactttcta
    23761 tcctccaatt caaatcagaa catcagttgt tgaatcgacg acctgaattt aaaatggacg
    23821 atcgtaaatt gttggaaaaa atcaactatg aggaatcaac gatcgtttta gaaggtcagc
    23881 tctatgagtt gaaagggact tgttttcaaa cgatccagcc agatgatcct aaaaaattct
    23941 tggaagagga acaaaaagtg atcgatagtt tgatgtattc attccagcac tcgttaagaa
    24001 tgcaaaaaca catgacgttt ttgatcgaaa aagggggaat gtatctcacg aacaatcagc
    24061 atgtactgtt ccatggctgt atacctgtag atgaaaaggg gcagctctta gcatttgaat
    24121 tagaccaatc ttatcgcgga aaagaactgc tgggattttt tgaaaaacag ataactgaaa
    24181 gcgcaaaaga tttgactgca aatgaagatt tggcgaccga tttgatttgg tatgcgtgga
    24241 gcgggccatt ttcgccttta ttcggcaaga gtaaaatggc aacttttgag cgctattttc
    24301 tcacagagcc tcatacgcac gtcgaaaaaa gcaatcccta ttatcacttg cgagatgaag
    24361 agtggttcag tgaaaaaatt ttggcagaat tcgatgcaaa agaagaaggt tcagcaatca
    24421 tcaatggaca cacgccagta aaagtaaaaa aaggagagtc accgatcaaa gcaaacggaa
    24481 aagcatttgt tattgacgga ggtctatcca aagcttatca aaaaacaacc gggattgctg
    24541 gctactcatt actatgcaat tcttacggtt tccaaatcgt gacacattat ccgttcttgc
    24601 ctattgaaca acaatttgca aaaaaatctg atcaaacgaa tgtaaaaaaa gtgatcgagc
    24661 agcagctccc acgcaaactt aatcggaata cgacaaaagg aatggaatta cagcaacaga
    24721 ttactcaact ccaaaggctg ttggattatc gaaaggacga ttgagaaaaa tgctagccaa
    24781 cattgctcaa tagactaaaa agcgactgca acaaaaggcg tgtcgcagtc tccttcggaa
    24841 ttagctcaaa aaacggtaaa atattagaag ctatttttct gtttatttct ctaatactca
    24901 ggtcagggcg ctgatttcac gacctcttcc ttaaacggtg ttctcctgcc aactcctcgg
    24961 tcacaagtca caatatccaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac
    25021 tcagagttga acggcaggct cacgacctaa tcagaaaaac gattaaaatt cattcacaaa
    25081 gaaatcgaat aaacgctgtt cagatgaatc tacacgatga gaaagttccg gatgccattg
    25141 gacaccaagg attttgtgca tattatcttt actttggact gcttcaatga gtccatcgtt
    25201 ggaaaaggcg atggctttta acaaaggaga aagatctttg actgcctgat gatgatagga
    25261 attcacaaag tatgaatcag ggagaaggtc acttaataaa ctgtccgaaa cgattttgac
    25321 ttcgtgagtt gcaaactgcg gggcagtggg ctgttgttcg tgttttacgg accattttgg
    25381 atataatgaa agatcttgat aaagtgttcc ctccaacacg acattcaata gttgaagtcc
    25441 tcggcagact gcaaaaatcg gttttctttg tttcaatgct tccaagatca atgcctgttc
    25501 gaattggtct cgttgaatat ttgtttccgt cagttttgga tgagggtctt ccatgtaaag
    25561 ttgcggacta acatcctgac cgcctgcaag aatgagtttg tcgattttct caattagttg
    25621 aggcgctgaa tctggagaca tgactggcag aataagaggt aatccttgag catctaaaac
    25681 tgcatccaca aatccctgcg gtgtatagct tacttgattt ccttggaaag tatctgtcgc
    25741 tcgaatcagt tgattcccgg caatgccgat gattggtttc atttttattc cccctaaagt
    25801 agactaattg taacaaggat ttaaaaaaat ttaaactatg taagacttgc ccctatactt
    25861 atctgttctt gctgtgttac tatctgtaaa gagaattttc gtgtgaaatt catgacgata
    25921 gttgaaaata ttgcttaatt tgttataata aaaagtaagc agaaataaaa aggttgggcg
    25981 taagcttcaa ggatctagtt cttcaagggg tgagaaggac attttattga aggggcctgc
    26041 ctatactaag ttatctttag aggtacatac tttcaatgaa aaaagaaagc atgtacctct
    26101 tttttaaatc gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca
    26161 gaagaagaaa atgagaagcg tgctcgaacc ctacggcagc agttgaagac ctatgcagaa
    26221 gaaaaagagc aagaattgga gataatccaa acgaacagtc ggcaacagct gaaacagttg
    26281 gtagaagaaa aagaacaatt cgaaaagcaa gaattagaaa aagaaaaaat gaaattgaaa
    26341 gcagaagcag acaaggtgca agcaactttg aaagaccaat atcaaaagca tcagagtcaa
    26401 gcaatcggtg caatcattga aagggtgaaa gaaacttatg gccgtcacta agatggaaaa
    26461 agtgaccctc atttcagata aaagaaatca ggagaggatt ttacaagcag tacaaggaat
    26521 ccaaaatgtt gaaatccggg atctgtatca aaatagaaca aacaaccagt gggtagaaaa
    26581 atattttcca gataccacga ttattgataa agaagcaaaa ttaagcgctt taggcaatcg
    26641 gctgaaagac atccaagaag ctatccaatt catacaacac cacggcgata gccgtcaaaa
    26701 gaaactccat ttgaaacgtc aagaactttc cttacaagac ttggaagcaa attattccga
    26761 agaaaatttc atgaataaat tagaagaggt ccttgattta aaagaaaagt gggaacagct
    26821 aactgaaaaa cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt ggcaacagct
    26881 ggatattgct ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg ggactgtgag
    26941 tgcagcaaat tttgaaagtt tcaaggaaga actaagtaag cttgatgacg tttatatgga
    27001 agaaatagat tatacgccaa aacaagttta tctttcgtat gttgctttaa aaaaatcagt
    27061 tgctgctata gaagaaattg ctgggaaata cgggttcaat aaagaaacgt atccttatga
    27121 acaaaatcct aaagaaaaat tgcaagaaac caaacagcaa ttatccattg ttcacgagca
    27181 gcaaaaaaag ataacgacag cgttaggtca gtgcagcggc tatatccaag aatttgaatg
    27241 ggtagaagaa gtcacacttg ccgttgcaga acgggaaaaa gtaaaagaac ggtttatcca
    27301 tgccacttat ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac agaatttgat
    27361 gcatgtgtta aacgaaactg tatcagaaga agatatatac gttagtttcg aaacaccaaa
    27421 ctcagaagag attcagcagg aagtccctac taaattgaaa aaccatccgc ttatcgaacc
    27481 ttttgaattg ctgactgaaa tgtacagtct gccaaaatat gaagaagtag atccaacgcc
    27541 ttggatgaca ccattttatc ttgtcttctt cgggatgatg gtcgcagatg ttggttacgg
    27601 attgctgatg ctcattggga caatactagc tcaaaaacta tttgttttac cccgcgggat
    27661 gaaacgtttc gtaaaattct ttgagattct ctcggtacca tcgatcatct ggggattgat
    27721 ctacagttca tttttcggac aggcattacc aaaagaacta tttggtatcc gcttgccttt
    27781 cccaatccta tctacgacgg atgacgtcaa tacgattttg attttatccg tgattttcgg
    27841 tctgatacag atcttggttg gattattcgt ggcagcaaag gaaaatatca ggaggaaaga
    27901 ttacttgagt gctgtcagtg atggttttgc ctggcaaggg atcctgattg ggatcgttat
    27961 tgcattgatt ggtgtcatgt tgataaaaaa tacagcattt gtttatcttg gcgcggggct
    28021 atcgatcatt gctgctttat gtatcttaat cgtgccgatt atccaatcgc cgtctaaagt
    28081 caagggtgct gccaaaggag cgtataatct ttacggattg actggttata tcggtgattt
    28141 agtcagttat acacgtttga tggcactagg tatttcaggc ggaagtattg gcgcggcatt
    28201 caatatgcta gttgctttca tgccgccagc cgctcgattc agtgtaggga tccttttgat
    28261 catcgcttta cacgcactga atatgttttt gacactgtta agtgcctatg tccatggtgc
    28321 acgattgcag tatgtcgaat tttttggaaa attttataca ggaggcggac gtgcctttga
    28381 cccgctgaaa acggcggaaa aatacgtcaa catcaatcat aagaaaaaaa acaataaata
    28441 attggaggat ttttatacaa tgatggatta cttaattagt caaaatggtg gaatggtatt
    28501 tgcagtatta gcgatggcaa cagcgacgat tttttcaggg attggttcag ctaaaggtgt
    28561 tggaatgaca ggtgaggcag cagcagcgtt aacaacaagt caacccgaaa aattcggaca
    28621 ggctttgatt ctgcaattgc ttccaggtac gcaagggttg tatggattcg ttatcgcctt
    28681 tttgattttt atcaacttag gaaatgatat gtcagttgta caaggattga actttctagg
    28741 cgcttctctg ccgattgctt ttacaggttt gttttcaggt atcgcccaag ggaaagttgc
    28801 agcagcaggt atccaaattt tagcgaaaaa acctgaacat gctacaaaag gaatcatttt
    28861 tgctgcgatg gttgaaacat atgcgatttt aggtttcgtt atttccttcc tattagtatt
    28921 gaatgcctaa gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat catctctcag
    28981 atgaatgaag cagcagagca agaaagagcg gcgttagagc aagaagagcg aatgaagatc
    29041 gatcaaaact ttgaacaaaa acgtacgcaa atcgaaacag aacatcaaaa acaaaaagaa
    29101 aaacaaatcg aattacttga aaaaaaatac cgtcagctta ggaatcgcca acaagtagaa
    29161 gtacgacaag aaaatttgaa tgcgaagcag gaatttttac gacgattgtt tgcagatgca
    29221 atagctgaaa tggagaattg ggatgaatct gaacaaatcc agtttatcaa aaattcattg
    29281 tattcattac ctttgacagg caaagtaaca ttcattgctg gagaaaaatc agcagcttat
    29341 ctttctcaga cattgctgga tgaatggaac aatgaacttc cctttatgat ggttttaagt
    29401 gatgaaactg ttgctgatca agcgggtttt ctgataaacg atcaaggagt tcagtataat
    29461 tttcttttca gcagccttgt acaagatatc caaggaacga tgagttttga gattgccaat
    29521 aaattattcg aataaggagg gatcagtgta tggaatacca cgaactaaat cccttgattc
    29581 gcggaagaga gcttgaactt ttgtccaatg agacatttga acggatgatt caaacaaatt
    29641 ctgtcgaagc acttggtgaa atattgaaat ccacgattta cagtccatat atttatgacg
    29701 gatatgaata tgacttcgaa cagaatcttt taaaggagca gagccgtcta tttcgctggt
    29761 taaaggaatc agcaccggag cctgaaatcg tttggatcta tacgatgcgc tatactttcc
    29821 ataatctgaa ggttttgaca aaagccgaga tgacaaatca aaatctggac catttgtata
    29881 tcgacgatgg attttattca ttagaaacct tgaaggatgc cattcatacg caagtttcaa
    29941 atgaactccc tgaaagcatt atggacagta tacgagaagt acgtgattat tttgaagagt
    30001 ctaccattct tcagggaatt gacgtgattt atgatcgaca gtttttgaca gagcagcgac
    30061 ggcttgccga taagctagga tatcaagaat tgctgacaga agtcattgct tttattgact
    30121 tgaccaatat cacaacgatg gctcgaggaa tattgcagaa aagaacatat gggttcatga
    30181 cgacggtact ttccagctcg ggaagtatcg aaaaagaagt atttttgaac tttgtagata
    30241 aagatatgga agattacacg aattttctat tagagacaga ttatgctgat tttctgcgcc
    30301 ctgtcatccg tgagggaaat cttgatctcg taaagcttga acagttgaaa gatgattatc
    30361 taagttcatt gtatcaagaa gcacaaaccc aggctttcgg accactgcca ctactagcct
    30421 ttttgaacgc aaaagaggta gaaagcaaaa accttcgttt actggttatc ggcaaaagaa
    30481 gccatttcga accagaaaca atcagaaagc gggtgagaca agtctatgac gcataagatc
    30541 ggtgtagttg gcgacaaaga ttctgttttg ccattcaaat tgtttggctt tgatgttcgt
    30601 tatggtacaa ccaagcaaac cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat
    30661 ggcgtgattt atgtcacaga gcaatgtgca gcattgagcc cagaaacgat cgaacgttac
    30721 aaggaacaaa tgatacctgc tatcgtattg attcccaatc atcaaggatc attagggata
    30781 gggctggaag aaattcaaaa aaacgtagaa aaagcagtag gtcaaaatat cttatagtat
    30841 gaaaaggaga tagaaatttt gcaaattgga aaaatcataa aagtctccgg acctcttgta
    30901 atggctgaga acatgtctga tgccagtatc caagacatgt gtctagtggg agatctagga
    30961 gttatcggag aaattatcga gatgcgtggc gatgtggcgt ctattcaagt atatgaagag
    31021 acttctggaa ttggtccagg cgaaccggtt cgctctactg gtgaagcttt atctgttgaa
    31081 ttaggaccag ggatcatttc acaaatgttt gacggaatcc aacggccatt agataccttt
    31141 atggaaatca cacaaagtaa tttcttaggg cgcggagttc aattgcccgc attggaccat
    31201 gagaaaaaat ggtggttcga accaacagta gaagccggag aaacggtgag tgctggagat
    31261 gttatcggga tcgtagaaga aacaaaagtt ataaaacata aaatcatggt gccaaatggg
    31321 ataaaaggta cgatcaaaac aatcgaaaaa ggttcattca ctattgatga aacagtatgt
    31381 gtcattgaaa ccgaaaatgg tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga
    31441 cgtagtcggc caatcaaaga aaaattaaac ccggatgcac ccatgatcac tggacagcga
    31501 gtgattgata cattcttccc agtgacaaaa ggaggagcag cagctgtacc tggtcctttt
    31561 ggtgcaggga aaacagtcgt tcagcatcaa attgcaaaat gggccgatgt ggacatggtt
    31621 gtctatgtgg gatgtggcga gcgtggaaat gagatgacgg atgttttaaa tgaattccct
    31681 gaattgattg accctaatac aggagaatcg ttgatggaac gtacggtatt gattgctaat
    31741 acatcaaaca tgccagtagc agctcgagaa gcttctatct atacgggaat cacaattgcg
    31801 gaatatttcc gtgacatggg ttatgacgtt gctatcatgg cagactccac ttcacgatgg
    31861 gcagaagcac ttcgtgagat gagcggacgt ttggaagaaa tgcctggtga tgaaggttat
    31921 ccagcttatc ttggttcacg gcttgcggaa tactatgaac gatctggaag agtcattgca
    31981 ttaggatcag aaggtcgcga aggaagtata accgcaatca gtgctgtatc gccgtctgga
    32041 ggggatattt cggaaccagt cacacaaaat acattgcggg tcgtaaaagt attctggggt
    32101 cttgattcca gtttagccca aaaaagacat tttccttcga tcgattggat acaaagctat
    32161 tctctttatt caacagaagt agggaagtat atggatcata tccttcaagc tgactgggca
    32221 tccatggtca ccgagggcat gcgtattttg caggaagaac agcagctgaa tgaaatcgtg
    32281 cgtttggtag ggattgattc cctttcagat aatgaccgtc tgacattaga agtggcaaaa
    32341 tccattcgtg aagattattt gcagcaaaat gcttttgatg atgtcgatac attcacttca
    32401 agagagaaac aattcaaaat gttgaagatg atcttgacct ttggtgaaga aggacgccgt
    32461 gcgttgtcgt taggtgctta tttaagtgag atcatggacg gtaccgtatc gattcgtgaa
    32521 aggatcggac gaagcaaata tatcccggaa acagaattag aaaaactaga tcaaattatt
    32581 ggtgaaatca agcaaacgat gcaaacgatc gtttcagaag gaggcatgag caatgattaa
    32641 agaatatcgt acgatcggtg aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg
    32701 tgtcaaatat gaagaattaa tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca
    32761 agtattggaa gtccaagaag ataaagcaat ggtccaaata ttcgaaggaa caagtggtat
    32821 caatttacgt gactcttctg tacgattttt aggccatcct ttagaactag gtgtatcgga
    32881 agacatgatc ggtcgagtat tcgatggttt gggacgaccg aaagataacg gaccagagat
    32941 cctgcctgaa aagcatttgg atattaacgg agaagtaatc aatccagtag ctcgggatta
    33001 tccagatgag tttatccaaa caggtatctc tgcaatcgat catttgaata cattagttcg
    33061 tgggcaaaaa ttaccagtat tttctggctc tggtcttcct cataaagaat tagctgctca
    33121 gatcgcgcga caagcaacgg tactcgattc atcagatgat ttcgccgtag tctttgctgc
    33181 tattggaatc acttttgaag aagcagaatt tttcatggaa gattttcgtc aaacgggcgc
    33241 aatcgaccgt tctgtcatgt ttatgaattt agcaaacgac cctgcaatcg agcgtatcgc
    33301 tactcctcgt atggctttga ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt
    33361 tttggttatc atgacagaca tgaccaacta tgcagaggca ttgcgagaga tttctgcagc
    33421 acggcgagaa gttccaggaa gaagaggata tcctgggtat ctttacacga acttagctac
    33481 tttattcgaa cgagcaggaa gaatccgcgg actaaaagga tcggttactc aaattccgat
    33541 tctaacaatg ccagaagacg ataaaactca tccaatccct gacttgactg gttatattac
    33601 agaaggacag atcatcttga ctagagagct ttacaagagt gggattcagc caccaattga
    33661 tgtactcccg tctctttcgc ggttaaaaga taagggaact ggacccggaa agacaagaga
    33721 agaccatgca gctacgatga atcagctttt tgcagcctat gcacaaggaa aacaggcaaa
    33781 agaattggcc gtagttttag gtgaatctgc tttatctgat gtagacaaga tctatgcgaa
    33841 gtttgctgag cggtttgaaa aagaatatgt caatcaagga ttctacacga accgttcgat
    33901 tactgaaaca cttgatctag ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa
    33961 acgaatcaaa gacgacatgc tggacaaata tctgccagaa gggaagtgag ttctgatggc
    34021 acgattgaat gtgaatccga cccgcatgga attgactcga ttgaaaaaac agttgacaac
    34081 tgccacaaga gggcataaat tattaaaaga taagcaagat gaactgatgc gtcaattcat
    34141 cctgttgatt cgaaagaaca accagctgcg tcaagaaatg gaagagtcta tgacagaagc
    34201 aatgtctgat tttgtattgg caaatgcttc gatcacggag cctttcatcg aagaattatt
    34261 tgttttgccg gcggaaaacg ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa
    34321 agtacctgtg atgaattttg aatatgatga acagctgaca gacacacctt tggaatacgg
    34381 gtatttgaat tccagtgccg aactggatcg gtcaatcgat cggtttacta ccctgcttcc
    34441 aaaacttttg gaactgacag aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga
    34501 aaagacacgt cggcgagtca atgcattaga atatatgacg atccctcaat tagaagaaac
    34561 gatctattat attcgtatga agctggaaga aaacgagcgc gcagaagtaa caaggttgat
    34621 caaagtaaaa aatatgggta ccactgacta agtggtatcc ttttttaaaa gaggaaaacg
    34681 atgattaaac aaagcaaaca aataaagaaa cgcttgccgg ctgttcagct tctcgcatta
    34741 ggattttttc tcctgatttt agttggcggc ggcctactta ccctaccatt ttttagtaat
    34801 agtgggcatg ggacaaattt tatcgatgcg cttttcactg caacttctgc tgtctgtgtg
    34861 acgggattga caacattgaa tacagcagag cattggaaca gtgctggtca atttttgatc
    34921 atgcttttga ttgagatcgg cggtcttggt tttatgatgg ttccgattct atgctttgca
    34981 ttgataaaga agaaagtcag tctcagcaca cgtattatat taaaagaagc gctgaatcta
    35041 gaagaagtat caggtgtgat gaagctcatg ctttacatcc tgaaattggc ctttgtgatc
    35101 caactgattg gtacggttgc tttatctttt gtattcgtgc ctgaatttgg ctggttgaaa
    35161 ggtatctggt acagtttgtt ccatgcaatc tctagttttt gtaatgcagg gtttgatttg
    35221 ttaggagaca gtttagccaa tcaccagcaa aatgtttatt tgatcatggt ggtatctgct
    35281 ttgatcattt ctggtgggtt aggcttcatc gtatggcgtg atttactgga atatcatcag
    35341 aaaaagaaga taacgattca ttcaaaaatc gcattaaccg ttacaggtac ccttttatta
    35401 ggcggattcg ttgtgttctt tttcacagag cgcaatgggg aaacgctagt ggaagggaat
    35461 atgtttcaac gattggcaaa cacctttttt atgagtgtga cacctagaac ggctggatat
    35521 tattcagttg attacctcca tatgagccat gcaggtctga ttttaacgat gtttttgatg
    35581 tacatcggtg gaacatctgg gtcgacggcc ggcggattga aaacaactac gttaggtgtg
    35641 ttgctgatcc agatgcgttc gatgcttaaa ggacgtacga gagcggaagc atttggccgt
    35701 accatccgac aaggcgcagt gttgcgggca cttactttat tttttgttac gttgacgttg
    35761 tgtgtagtcg ccatcatgat tttatcggtg actgaaacga tacctaaaac ttctggcatc
    35821 gaatatattg cttttgaggt cttctctgcg tttggtacag taggtcttac gatgggattg
    35881 acgccagact tgactgcaat agggaaattg atcattatag cgcttatgta cattggacga
    35941 gttggtgtat tgactgtggt cttctctctg atgacaaaag cacaaagaac acaatcaaaa
    36001 tacaaatatc cagaagaaag tgtcatgatt ggataatttc atcttctgac agaaacctag
    36061 tgatttagta aaataagaaa ataaaataat ttttcttgct tcttggatac tttaagtgca
    36121 aaatgttcgt ttattttttg aggcactatc cgttgttgaa cctcgggtag tgcctctttc
    36181 ttattgaaag gaatcaataa gatttgattc ctttatttat caataggtta taattcatat
    36241 ttttttattt tttcttttac gatacttagt ccagaagcta aaatttcaag gttattagag
    36301 gatagggaaa ttcgaacgaa tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga
    36361 taaagtcgga taccgttagc aagaaagtca ttctcaatta tcttaggatc aatctcagga
    36421 tcaactggaa gaattcgata gtaagggaca cgattcgttg gtttttctac tttgaaaaga
    36481 gaatcaaaaa gctgattggc tcttttcgta agacttaatt tagtttctaa gatcctattt
    36541 gcttcatttg aatggaggat ttgagtgatg acttccacat ctaatccaga agttttcaca
    36601 tttatattaa aaattgcttg ttctatcttg ttttggacat gtactggaac tacaagataa
    36661 gcaactcgca atccagaaca aataaatttt gtcattccag ctaagtagat tgtttggtta
    36721 ggtaataatt tttggaaagg aggaatgatc gtatgttgat agtaagttgt taaaaatgaa
    36781 tggatatcat cttcaataac ccatagacct tctttttcaa tcacttcggc taattgctct
    36841 cgtcttttgt gagagatttg aaaccctata ggattattac aagaaggcat aagaaagaca
    36901 ccatgaatct tcttttttct acattcctga atcaatatat caggtcgcat tccttgctcg
    36961 tcaaaatcaa ttggaacgat ctctaaatga tataactgag ccaattcaat gaaattagcg
    37021 tatgtatatc ggtctgttgc tattctatca cctggagaga aaagtgcaga aagtgtgata
    37081 gccaatccat tttgaacacc ggaaacaata gatagagatt ctaaggacgg aatgactcct
    37141 tgagttttta accaatttgc tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg
    37201 taattaagca aaccaacaag atttgggtta ttactagttg attgaataaa cgggacaacg
    37261 aatttattac attcttcaaa agaactaacc agaccaaaat caatgatatg ttcggttact
    37321 tcattggtag agatggtgat ggactcaaag acattgggag aaataaatgt gccacttcca
    37381 atattcgtat acagcaaacc tttctcaatt ccatatttat aagccttgcc aacggtagta
    37441 aaatttaaat ctaaataatc agctagctct ctttgggagg gtaactgagt gtttttctgt
    37501 aatttattct ttaaaatatc ttgttctaat tgttgaatca acgatcgata ataaggacgc
    37561 ttcaattttt gtttgtctgg tcgccaagtt aacgggtaat tgtcataaga attaataggc
    37621 attttctaca ctccttaaaa ttgtatacca tacaatttta ccattgattg tattgatttt
    37681 agaagttaat cttttgtgag gagggaattg tctatgaaaa ttattaattt tgaggaagta
    37741 gtagcttctt ttagttttga agaagcgatt caagtaatga aaaaatgttt ttttgattat
    37801 gaaagtggta aaatcagcca aaatcctcga ttggtggaaa ttttacctga cggaaaagac
    37861 aaaaatatgt ttgcgatgat gcctgcttat ttgggggaga acagatattt tggaacaaaa
    37921 gtcattaccg cttttccgga taatcatcag tatcatttac cttctcattt aggagagatt
    37981 ttcttgtttg atgctcaaaa cggactaccc aaagcattga taaacgcaaa tgcagtcacc
    38041 tggattcgaa cagctgctgt atctgcgctg gcaacagatt acttagcaaa tccgcatgca
    38101 gaaacacttg ctttaattgg agctggtcaa caagcattgt cgcatttgac tgcgatttct
    38161 accattcgtc ccatcactac agtgtttgtt tacgatattt tagaagaaag acgaaatact
    38221 tttatcgcta aagctaaaaa acagtatcct aaaataacgt ttataaatgc aatatcagta
    38281 gaagatgcag tacgtccagc ggaaatcatt tgtacgttga cctctagtaa agaggcattt
    38341 cttcaaaaag agtgggtaag ggaagacgca catatcaatg ctgtgggcac atttactcct
    38401 gatacaagag agataagtac atcgttgatg gaaacttctc aaatctatgt agatgattac
    38461 tcagctacct taacagaaag cggggacctg cttatagcca tggcagaagg agatttagag
    38521 gaatcttgta ttgttggtac attaggcgaa ttagtttctg agaaaaaaac attttataaa
    38581 ggagaatcga ggccgacgat tttcgacgca gttggtctag ctattgaaga tctttgttgt
    38641 gcagaatata tttataacaa attagagaaa ggggctttaa aatgaactta gattattacg
    38701 tagttgatgc atttgcagat gaggtgttca aaggaaatcc agcagcggtt tatgtgctag
    38761 aagaatggct gccagaaggt acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa
    38821 cagcatttac agtgaagaaa aaccaagaat ttgagcttag atggtttacg ccagatcgag
    38881 aaatcgatct ttgtggtcac gcgacattag caacagcatt tgttttattt aactattaca
    38941 aaatacctga tgaaacgatt aaattttcta ctcaaagcgg taatctattt gtgacaagaa
    39001 ttaaagatta ttattacatg gattttccta gtattatgcc gaaaaaagtg ccgatattga
    39061 ctgaatatga agaagcaatt ggggctaaga ttaaagaagc ctacttagca cgtgatttat
    39121 ttttcgtatt agaagatgaa aaaacggtgg ctaaattgca gccagatttt acagctatca
    39181 aaaattttga attaggaata gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt
    39241 ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca
    39301 ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca
    39361 aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg
    39421 aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt
    39481 aaaaataaat tggtggagag tggcagcctt atagctacaa tggttgttaa aaaaagacgc
    39541 aaactttttc aatttaatga aaagtttgcg tcttttttta attattggtg tttgtttttt
    39601 attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac
    39661 catcagggtc aatgacaaag tagtaagaag gcgtagtgcc aggtaatcct tttaagtcag
    39721 taatctcaag acctgcagct ttatgttgct cgtgtaattt ttctaaatca tcagctgcaa
    39781 tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca
    39841 actctaattc atagtcatct ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg
    39901 gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg
    39961 cttcaagatc tttcacccga acacatgtgt gagccatttt cataaattca tctctccttt
    40021 tccttttact caatcataac aaaaagaagt aagaaaatag ttgatttgtg cttgttcatg
    40081 agaaatttct catgtaaaat ggatagaaaa taatactgtt aaggtggaaa caattcatgg
    40141 ataaaccaat ttttggtgaa aaaaagcaat ctgctaatta tcaaacccgt tatgcagcat
    40201 atattgtcat agaaagagac aagcggattg cggtgatcga ggctccaaat ggtgcttttt
    40261 tccttcctgg tggggaaatc gaaggagaag agacgaaaga agaagcgatt gtccgtgagc
    40321 tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt
    40381 atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg
    40441 attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc
    40501 ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga
    40561 tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt
    40621 tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg
    40681 atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca
    40741 gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga
    40801 tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt
    40861 agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc
    40921 ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg
    40981 atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa
    41041 cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca
    41101 tcgatcgaat ggagatgact gccgacacgg cgtgccatca cttcgatctc taggaaatct
    41161 tcatgagtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc
    41221 ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc
    41281 tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca
    41341 gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca
    41401 tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt
    41461 tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa
    41521 tcataatgat cacgcaagga gggatcaacc atttcttttg gaaagaaaaa tcgctcatct
    41581 ccttgttctt gccccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa
    41641 gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa
    41701 aaatcataag gcagatcgta gctagaattc acagcagtgt aataaacagg gttatagcca
    41761 accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa
    41821 gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga
    41881 tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg
    41941 tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca
    42001 aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatc
    42061 acttccatac cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta
    42121 acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa
    42181 ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa
    42241 taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg
    42301 acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct
    42361 tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca
    42421 gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga
    42481 ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct
    42541 ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa
    42601 atctcataaa cgcctaatga atgggtaaaa cggctatgtt ctgctccgtg aaaagtgaaa
    42661 gaagaagtgc ctaattgttt aatgcgtcgt aaacgttgga cttctttcga attgatcaaa
    42721 tctaagatta ctttatgctg gacatggata tagttatgga ctggatcacg aaaaactttt
    42781 tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt
    42841 acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag
    42901 ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa
    42961 tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat
    43021 tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg
    43081 ataaaatcgc ttcatgactt gcccgcgttt ttcctgatta ccgttgacac ttaagtacat
    43141 cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg
    43201 gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg
    43261 tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt
    43321 tatttttttt gtgtcacttt gagggaaaat caaagaaata tttaaaattc cttcatctga
    43381 tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat ctttcagtgt
    43441 ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca
    43501 gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat
    43561 atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat
    43621 cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc
    43681 taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac
    43741 aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa
    43801 ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa
    43861 gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac
    43921 atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta
    43981 gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt
    44041 ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct
    44101 acttttacga aagaactgca tgttagtttg aaagaccgtc ccacagcagg gaaggttcga
    44161 atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat
    44221 tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg
    44281 agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa
    44341 agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg
    44401 gatttttctg atttagctaa ccagatccaa aactatcttg agaaatcaga cagtgaaatt
    44461 cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta
    44521 ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta
    44581 aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc
    44641 aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat
    44701 acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa
    44761 gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat
    44821 gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat
    44881 gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac attattttct
    44941 tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgatttt
    45001 tgtcacacct tcttttttgt atataaaaat cccaagtaaa tacataaaaa ataaaaaaat
    45061 tgaaactttt agcgcacctt gtacgtcata ttaagtgagg cagaaaggag tggagaatga
    45121 tttttactgg atataagaaa aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac
    45181 tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat gaattacgta catgataaag
    45241 aagaggcctt agatgtgatg caagatagtt ttcataaagc cttgaactat tttgaaaaag
    45301 aaaaagagat cgagcatttt agtgcatggt tttatcggat attgatccgc acggcattag
    45361 atagttggcg cagacaaaaa aggacgcctg tggacttatt tgaaattcaa gatatccccc
    45421 aacagttacc tttagaaact tccgttgcag agctccatat gattttagat aaaatagaca
    45481 gtccggaaaa agagattctg atcctctact tttttgaagg atttcgtttg aaagaaattg
    45541 caactatctt ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg
    45601 ctttacgtca aatcctagaa tgaaggagag aagagtgcca tgtattccca aaaagaaaat
    45661 ttaaaaaaac taaagaaaac ttataaagaa caagcaattc ccaaagggct taaagaggag
    45721 atccgccagc gttttattat cgaagagcaa tcatttataa aagcaaggcg gagaaagaga
    45781 cgtctccaag ggttcggagt atctttggct atcgtaatga ttacaacaag tagccttctt
    45841 tttaatagtc aagttcgaag ctttgcagaa gatttaccca tcttaggatc agtcattgaa
    45901 ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa tcgatattca ggtaccgaag
    45961 atcaatacac aaaatgagaa agaaaataaa acaattcatg gattaaatca aaaatacttt
    46021 cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat atggaaattt tgaaacggat
    46081 cattaccaag ttttgggaga ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt
    46141 gaaaggcaga tcacccaaac agcagcagat agccatgttg aaaaaagata cgacacaatc
    46201 gacaagaaaa acagtgtcca gctctcactt ccacttttat tcaaagatga cacttatcta
    46261 tctgttttga caaaagaagt caaacggcag atggccgaac aagtcaagga agatccatct
    46321 aagtactatt ggactgagca agatattcaa gaagggacga tagaaaaacc aacacttgtt
    46381 acgcctacac gaagttttta tttgaacaaa gagcatcaat tagttttgac tttttctcaa
    46441 tacgaaattg ctcctggtta tatgggtaca ccagaatttg tcattcctaa gtccgtgaca
    46501 aaaacgattt tggcatcgga agattatttg gatcattaga cgagaagtag ttgcgactag
    46561 cagtttcaat tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga gaaagaagag
    46621 atatagtgaa aagaaattat ttagtcatca gtattattgc tttatccgtg cttttgtttt
    46681 tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca cactcttcgg
    46741 agcattcgtc acaatctttc aaacaatcag aacaatcaac cattaaaagc caatcaactg
    46801 aaacagctaa cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa agtcaatcat
    46861 cacaatcaac acaagacagt acagaagcaa tccaagaccc gactgtcaaa agcgtcgcaa
    46921 tcagctttga tgatggacca ggagcgacca caacgccgca gcttctgcga atcttaaaag
    46981 aaaaaaacgt gcatgtcact tttttcgtct taggagaaaa tacggcgcag catcctgaaa
    47041 tcgtgaagca gacagctgaa gcagggcacg agattggcaa tcacacatac gatcatcaga
    47101 atctggccac tctttctgcc cagtcgatga cggaggaagt gacgaaagca gacactgaga
    47161 tcaaaaaaag tcactggcaa aacccccgct tttgttcgtc cgccctatgg ttctgtcacg
    47221 aatgtaggag caacgatcat ccagcgaccg atcatcgaat ggtctgttga ttcagaagat
    47281 tggaagacaa ggaatcctga tttgattttg cagaagatcc aagcaacagt atacgatggg
    47341 tcaattattt tattccatga tatctatcct gaaacgattc gtgccgttcc tcaagtgatc
    47401 gattatctga aagagcaagg ctatcgcatc acgacagtta gcgatttatt agggcatcca
    47461 acggctgtcg agaactatta tggcagaaac gatcatcggc cagttcagta agtgagacaa
    47521 gtatctttaa taaataacac attttaataa gtagttgaac acataactac cttacttgca
    47581 aaggcttaac aatatctata cagatacaac aaaaaactgt ccaccaattc tctttggcgg
    47641 acagttttca cttaaaaata aagaagcaac tattcgtaaa ttatgctaaa atagcagtag
    47701 aacgcgtcct gagggaatcg aacccccgtc tcaagaaccg gaatcttacg tgatatccac
    47761 tacactaagg acgcaagcac tgggataatt gtaactaaac agataaaaaa ttacaagggg
    47821 attggacaaa aaaatgaaat ttgagcaaaa tttttcacaa aaacagcagc agacacagaa
    47881 actagccatg acgcaacagt tgcaacagtc catccaagtt ttacaatata acagtgagga
    47941 gctgttggca ttcgttgaga atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc
    48001 ggaatggcag ccagattaca taaaagcttc ttcatcttct tatgaaggag aagagacaaa
    48061 ttacctaaat cagattcctg atacaaaagg gtctttattt gattcattga tcgaacaagt
    48121 ccatttgaat taccgagata cttttttgag gaaaatcgtt ctttatcttg tagaatacat
    48181 cgatttgaac ggatttttga cgattagttt agaagaagcc ggaaaagaga ttggagctac
    48241 gcctatccaa atgctcgatg cgttgactct gattcagcaa ttagaaccag ctggagtagg
    48301 tgcacgtagt ttgcaagaat gtctcatgct tcaaacagaa cgagatgatt atgcgccgga
    48361 attagcttat atcgtgttgg aagaatgttt tgaggaactg gtggagcgaa aatggaaaga
    48421 aatcgcacaa cgtttttctg ttgatctaca tgctgttcag cagatattcg actatttaca
    48481 aacgttgagt ccaagccctg gccggatttt cgaccgtagc agcgaattgt acattattcc
    48541 tgatgtccgg gtattagtgg acgacgataa aaatgtacaa gttatctcaa accgaaaaaa
    48601 tcagccaaat atacgctttc aagagagtta cttcaaacaa atgtcacaac aagcagataa
    48661 agaaactgaa aattatttaa aagaacggaa acaggaattc gaatggttga agaaaacgat
    48721 cctccaacgt ggggatacaa ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc
    48781 tttctttatc gacaaggaac gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga
    48841 gatagatgtg catgaatcca cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga
    48901 tttcggtgta tttgaattaa aaaaattttt cactacacga attcctacga atagtacaga
    48961 acagacagaa gacctttcag cagatacagc gaagaaaaag ctgcaggaat tagtcgatca
    49021 agaagacaaa aacaagccgt tgtcggacca gaaattggtt gaattgctga aaaaagatga
    49081 gatagcgatc tcaagacgaa cagtagcaaa atatcgtgat ttattaggaa tcccgagttc
    49141 atcaaaaaga aaacgttatg ataactaatc agtcaaaaga ctagaaaaag tatttcactt
    49201 tttctagtct ttttagttga aaaacgagag caaaactgtt agacttacag tcgtgaggtt
    49261 gagatttccc tttattgatg tgaaattcga atagttaatt tcttttttta tcgtatatgg
    49321 gtcgttttaa gtctacattg gacgtaaagt gtccaactaa ggagatcgct atgctggatg
    49381 aaataaaaat gattgaatct gttgcaccag atattatcga agtgttgcaa gaacgctata
    49441 aaattttacg aaatatctat tggatgcagc cgattgggcg tcgaagttta tcggaaagta
    49501 tggggatcac agaacgagtc ttacgaacag aaacagacct tttgaaaaat ctgaatttga
    49561 tcgacacctc aaaaagcggt atgacgctga caactaaggg cgaagaagtt tatcaaagcc
    49621 ttgaaaactt catggatcag cttttaggta cgcatcaaac cgaacaacag ttggcagaat
    49681 attttggtaa ccaacgctgt atcgttgttt caggaaacag cgaagagcag accaaagtgg
    49741 ctgatgcatt cggcgaagcg ctgagcgaag ccttagaccg actacttcca gaaggcgaga
    49801 atatcatcgc tgtgatggga ggaactacta tggctgtggt agccgaacaa ttgtctaatc
    49861 tggaaaataa gaaacggcat aacctgtttg tccctgcaag aggcgggatt ggtgaagcga
    49921 taactgtaca ggcaaattct gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc
    49981 gcgcacttta tgttccggaa caattaagtc tggcaactta caattcttta ctcaatgagc
    50041 catctatcca agaagtctta aacttgatta gtgaagcgaa ttgtgttatt catagtattg
    50101 ggcgtgcatt acacatggca gcacgtcgta agatgactga aaaagaatta gtcatgctga
    50161 aacaagcaaa tgctgttgcg gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg
    50221 tttataaggt ccctagaatc ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca
    50281 tggcaatcgc gggcggaaaa tcaaaagcta aagcaattcg agcttatatg aaaaatgcac
    50341 caaaacaaac gtggctcatc actgatgaag ctgctgcaaa tgagatttta aaaggggtaa
    50401 ccctttaaaa taaatatttt tttgattttc ataaggagga aatcttaaat gacagttaaa
    50461 gtaggtatta atggttttgg acgtattgga cgtctagcat tccgtcgtat ccaagatgta
    50521 gatggaatcg aagtagtagc aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg
    50581 ttaaaatacg acacaactca aggacgtttc aacggaacag ttgaagttca tgaaggttca
    50641 ttcaacgtaa acggaaaaga agttaaagta ttagctaacc gcaacccaga agaattacca
    50701 tggggcgaac taggcgtaga tatcgttcta gaatgtactg gtttcttcac ttctaaatca
    50761 gcagctgaaa aacatttaac agctggtgct aaacgcgttg ttatctctgc tcctggcgga
    50821 aacgatgttc caacaatcgt ttataacaca aaccacgaaa cattaactgg taaagaaaca
    50881 gttatctcag gtgcttcatg tactactaac tgtttagctc ctatggctaa aacattgaac
    50941 gacaaatttg gtgttgttga aggattaatg acaactatcc acgcttacac aggtgaccaa
    51001 atgactctag acggacctca tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca
    51061 aacatcgttc ctaactcaac aggtgctgct aaagctatcg gtttagtaat tcccgaattg
    51121 aacggtaaat tagacggagc tgctcaacgt gttcctgtac caacaggttc attaacagaa
    51181 ttagtaacag ttcttgaaaa agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa
    51241 gcttcaaacg aatcttacgg atacaacaca gacgaaatcg tttcctcaga tatcgttggt
    51301 atgacttacg gttcattatt tgatgctaca caaactaaag taatgacagt tggcgacaaa
    51361 caattagtta aaactgttgc ttggtacgac aacgaaatgt catacactgc acaattagta
    51421 cgtactttag aatacttcgc taacttataa gatagacaaa cgtacaagtg taaattatag
    51481 acatgtacat ttaaaagcgg ggaagcaatc gcgcttcttc cgcttttttt gataataagt
    51541 ttattcagga ggaaataaaa tggctaagaa aacagtgaaa gatatcgatt taaaagacaa
    51601 aaaagttctt gtccgtgttg acttcaacgt tcctttgaaa gacggcgtga tcactgacga
    51661 cactcgtatc aaagcagcat tgccaacaat caactacgtt ctagaacaag gcggaaaagc
    51721 aatccttttc tctcaccttg gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt
    51781 agctccagtt gcaaaacgtc taggcgaatt attaggtaaa gaagtaacat tcgtacctga
    51841 aactcgcgga gaacaattag aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt
    51901 attcgaaaat acacgttttg aagatattga cggtaaaaaa gaaagcaaaa atgatacaga
    51961 actaggtaaa tactgggctt cattaggcga tgtatttgtt aacgatgctt tcggtacagc
    52021 tcaccgtgca catgcttcta acgtaggtat cgcttcaact ggtatcccaa cagttgctgg
    52081 attcttgatg gaaaaagaaa tcaagttcat cggtgaagca gtagaagaac caaaacgccc
    52141 aatgattgca atccttggtg gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt
    52201 gattcctaaa gcagataaaa tcttaatcgg tggcggaatg acttacacat tctacgaagc
    52261 aaaaggaatc aagatcggta actctctagt agaagctgat aaagtagaac tagcaaaaga
    52321 attaatcgaa aaagcaggcg acaaacttgt cttgccaatc gacaatgtct gtgcaccaga
    52381 attttcaaat gatgttgaaa cacaagtaat cgaaggagac attcctgatg gtttgatggc
    52441 tttagacatc ggcccagcat cagtaaaatt atttgctgat acattacaag gtgcaaaaac
    52501 agttgtttgg aacggaccta tgggtgtatt tgaaatgagt aactttgcta aaggtacaat
    52561 cggtgtttgt gaagcaatcg ctaacttaga aggcgcaaca acaatcatcg gtggcggaga
    52621 ctcagctgca gcagcagaac aattaggatt cgctgacaaa ttcacacaca tctcaacagg
    52681 tggtggcgca agcttggaat tgcttgaagg taaagaatta ccaggactag cagcaatcaa
    52741 cgataaataa acgaccaagt ttgctcaatt ctgacgagta atcgatcaaa aggtgaaggg
    52801 agttttcatg actccctagc catttttcaa taaattaagg agtgtgttcc tcaatgcgta
    52861 aacctattat cgcaggtaac tggaaaatga acaaaactgc atcagaagca aaagaatttg
    52921 ctgaagcagt aaaaaacaaa atcccttcaa atgacaaagt tgattcagtc atcggatctc
    52981 ctgcattatt cttacaagaa ttaatgacat cagctgaagg tacagaacta aaaatctcag
    53041 cacaaaactg ctactgggaa aactcaggtg cgttcactgg tgaaacttca ccagcagctc
    53101 ttgctgatct aggtgttcat tatgtgatca tcggacattc agaacgtcgt gaatatttcc
    53161 atgaaacaga cgaagacatc aataaaaaag caaaagctat ctttgcaaat ggtatgacac
    53221 caatcctatg ctgcggcgaa tctttagaaa cctatgaagc aggcaaaact gctgaatgga
    53281 tcgaaggaca aattacagct ggtttagctg atctttcagc tgaacaagtg tcaaacatgg
    53341 ttattgctta tgaaccaatc tgggcaatcg gtactggtaa atcagctgac gcacaaatcg
    53401 ctgacgaaat ttgtggagtt gtacgtcaaa cagttgaaaa actttatgga aaagaagtat
    53461 cagaagcagt tcgtatccaa tacggcggtt ctgtaaaacc agaaaatatt gctgaataca
    53521 tggcaaaaga aaatgttgac ggtgctttag tcggtggcgc aagcttgcaa gcagattcat
    53581 tcttgtcatt gttagacgct gtaaaataat tgttgaaaat ttgtgaaaaa tgcaagaaat
    53641 gtttgcacta atgagcaaaa ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa
    53701 acaaactcaa aggagagaca aaacatgtca attattactg atgtttacgc tcgcgagatc
    53761 ttagactcac gtggtaaccc aacaatcgaa gtagaagtat acacagaaag cggagctttt
    53821 ggccgcggaa tggttccttc aggtgcttca actggtgaat acgaagcagt tgaattacgt
    53881 gacggagaca aagctcgtta tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac
    53941 aacatcattg ctgaagcaat cattggctac gatgttcgtg accaaatggc tatcgacaaa
    54001 gctatgatcg ctttagacgg aactcctaac aaaggtaaat taggtgcgaa cgctatttta
    54061 ggtgtttcta tcgctgttgc acgtgcagct gctgactacc tagaagtacc tttataccac
    54121 tacctaggcg gattcaacac aaaagtattg ccaacaccaa tgatgaacat catcaacggc
    54181 ggatcacatg ctgataactc aatcgacttc caagaattca tgatcatgcc tgttggcgct
    54241 ccaacattca aagaagctct acgtatgggt gctgaagtat tccacgcatt agcttcaatc
    54301 ttgaaagctc gcggattagc aacttctgtt ggtgacgaag gtggatttgc tccaaacctt
    54361 ggttcaaacg aagaaggttt tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta
    54421 cctggtaaag acgttgttct tgctatggat gctgcttctt cagaattcta cgacaaagaa
    54481 aaaggtgttt acgtactagc tgactcaggc gaaggcgaaa aaacaactga cgaaatgatc
    54541 aaattctacg aagaattagt ttctaaatat ccaatcatct caatcgaaga tggattagac
    54601 gaaaacgact gggacggatt caagaaatta actgacgtat taggcgacaa agttcaatta
    54661 gttggtgacg acttgttcgt aacaaacact caaaaattat ctgaaggtat cgaaaaagga
    54721 attgctaact caatccttat caaagtgaac caaatcggta cattaacaga aactttcgaa
    54781 gctatcgaaa tggctaaaga agctggctac acagcagttg tatctcaccg ttctggtgaa
    54841 acagaagatt caacaatctc tgatatcgct gttgcaacaa acgctggcca aatcaaaact
    54901 ggttctctat cacgtactga ccgtattgct aaatacaacc aattactacg tatcgaagac
    54961 caacttggtg aagttgcaga atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa
    55021 taattctttt ctaatgaaga tacatgatcg actattgaaa gatagtcgag tatgatcact
    55081 tagttaagta atcgaactct ctacttgctg aaatatcagc aggtagagag tttttttgca
    55141 actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat ccttgaaaaa
    55201 ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca gtagaatagg
    55261 aatctatgag caagtcctct cgtataaata aaataggagg gaagtagcag catatttttc
    55321 ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa attcgagcac
    55381 actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa gaagaaaaaa
    55441 tcgcgatttt acaagagatc atccgaatta aatcagtgaa tggaaacgaa ggtgaagtag
    55501 cagcctattt gaataagctt ttggcaaaac atgacataac aggagagatt gtttcttaca
    55561 gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga aaagtgttag
    55621 gcctatctgg gcatatggat gtcgtggcgg ctggtgatga atcgtcttgg acctatgcac
    55681 cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca gatatgaaga
    55741 gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga aaaccattta
    55801 acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta ggtggggaac
    55861 agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga gagccaacca
    55921 attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc tcgcacggaa
    55981 aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac ctaaatgaat
    56041 tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag aatcctgtcc
    56101 taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc aacagcatcc
    56161 caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca aatgataaaa
    56221 tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac tatcatttgg
    56281 aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct cctttgattc
    56341 attgcattca gcaacaattc agccagccat tacctttagt tggagcggca gcaacaacgg
    56401 atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt gggcctggtg
    56461 tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat ttggatatga
    56521 tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt aataaatgaa
    56581 aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca gcctattttt
    56641 ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac aacgacgata
    56701 gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact tgttgtctga
    56761 cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga ggtgatcccg
    56821 ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga caaaatcaaa
    56881 gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg caaaatcaat
    56941 ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa aatatctgta
    57001 gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac aggcccaagc
    57061 aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg aagccataaa
    57121 gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt aagtacgcga
    57181 gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca aatctttcat
    57241 aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa aaagacgagt
    57301 atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat aacgatcaac
    57361 caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt tgaaaccttc
    57421 acagatggcc tcctcgataa aataagcaac agctaacgga tcattgatga cacatcctaa
    57481 aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt ttagatactc
    57541 tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca agacgatttc
    57601 tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga atacataaga
    57661 agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt taccatggct
    57721 tttgtaagtt ccacccatcg agacaaaacg ttccatatgt ttcccaatat ttgggttgac
    57781 tttcaaagca gaagcgatat tggtcaaagg acccaaagca atgactgatg tatttgtctt
    57841 ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct gttttctcaa
    57901 gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt gtgcactgat
    57961 gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta aacgatcaca
    58021 gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat tcccacaaac
    58081 tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata aagctaaagt
    58141 gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca atctcctttc
    58201 tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag acgagcgtct
    58261 tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat gctctcttac
    58321 aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa atggaattta
    58381 cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagat agattatagt
    58441 ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat tctgataatt
    58501 atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt aagagcggga
    58561 gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga ccctgctgca
    58621 gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag cttttgggag
    58681 cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg tcagatgttc
    58741 atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg aagcacaggg
    58801 aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa cgttcttgta
    58861 cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg atggctagat
    58921 cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa tgcgtccatt
    58981 attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg tgaaaaactt
    59041 ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta tgcagaattc
    59101 cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag atatgcgaat
    59161 cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac gagacagctt
    59221 ggcagcaatc atctgccttt attagtcatc catggtgaaa gagatcattt tgtccctaca
    59281 gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct gcttgttccg
    59341 gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac agtgattttt
    59401 tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg taagtaattc
    59461 gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga ataaaaaaat
    59521 atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaatcg tcttgcttta
    59581 ttcaatgatg aacaacaaga gatcggagaa atgacatggt cagatgcggg cccagacatc
    59641 atgatcattg accatacatt tgtagatcca gcatatcgtg gtcaaaagtt agctgaaaag
    59701 ctagtgtata ctggagtaga attagcaaga cgagacggga aaaagatcat tcctttatgc
    59761 ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc aagacgtctt acgtcagtca
    59821 taagaataac agctgaaagt tttgaacttt cagctgttat tccatttata tagagttgtc
    59881 acggactttt atctatgata aagttaaaga gacgatatct tttgggaagg aggctgcgat
    59941 atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt cattgcgatt
    60001 atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg tgcggatcaa
    60061 ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc aactgcagtt
    60121 atgggagcta tctggatgat cttattattt gtcctagcat atttatcttc gaagtagttg
    60181 aataagcctc tcgcaaaacg gcgggcggct ttttttattg caaagagaac gcttaagaag
    60241 aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgagacgagt gagacaatta
    60301 ccaaaacccc tttatgcaaa acatggaaag cgagcggttt tgctgcttca cgcttactca
    60361 ggaagtccaa acgatgttcg aatgctcgca cgatttttgg aaaaagcaga ttatacagtt
    60421 tatgcgccgc tttttaaagg acacggaaca atggagccat atgatatctt gcaagaaaaa
    60481 gcagaaagct ggtgggcaga cactaaaaaa gccatccatt ttttacagtc tgagcagttt
    60541 tctgatattg ccgtattagg attatcaatg ggcggcatct ttgctgtacg agcactagaa
    60601 gaagaatcag tgattggcgg cggttttttc tgttcgcctt tgtctcctgt aaaaacaaac
    60661 gtgcctgaaa actttgaaaa atacgtacgt caagtgctga agacagcagg taaatcagag
    60721 aaagaaatca atgaaaaagc agttgcttac cgctcgttag ctgaacaaca attaatggat
    60781 atacaagatc aggcagcaat cgtagaaagt aggttgtcag atatccaaca gccgatattt
    60841 ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg gcgtatttga aactgcacgc
    60901 aagttgagcc gtcagcgagt gacacttcaa tggtatcctg aaagtggtca tgtgattaca
    60961 gttggtacag caagacgcga attagaaaaa gacgtcttgg aatttttaga aaaattacct
    61021 tggaatgagg aataaatatg acaaaagaaa cgataaaaga gaaaattctc tttttcatgg
    61081 aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc tgaaggttta gggttccaaa
    61141 aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga gaacagagta
    61201 tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa accaatcttg atcgaaggaa
    61261 catttcgagc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa gaagatgaca
    61321 tctatatccc aaaagaagcg actggttttg caatggatgg agacaccgta gcaatcgata
    61381 tcatgaagcg agcaaataca gatatggatc gcggagcaga ggggaaagta gtcgagattc
    61441 gagaccgggc gactactcag ttagcagggg aattcgttgc ttattcagaa gaagaaatac
    61501 aagaaacaga tctttacggc gttgttatcc cgaaagatag aaagctcaac caatttaaag
    61561 tatatgcagc agcagaggga gttcgtccag ttgacggaag tattgtgctg atcgaactga
    61621 cccattaccc tgaaaaaggg tatgcgacta gtttagaagg gataatcaag caagtgattg
    61681 gtcataagaa tgaccctggc atggatatcc tctcaattgt cgtggctaat ggtatcccta
    61741 cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt tccagatcaa atcagcgaaa
    61801 aggatataac tggacgtaga gatttaagaa atcagctcat tgttacgatt gatggagaag
    61861 atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt agataacggt aattattttc
    61921 ttggagtaca cattgcagat gtttcttact atgtgacaca aggcagtcaa ttggacagag
    61981 aagcatatga aagagggaca agtgtatatt tgactgatcg agtaattccg atgatcccgc
    62041 aacgtttatc gaatggtatc tgttcactga atccccatgt tccaaggctg accatgagtt
    62101 gcgaaatgga gattgatcca aatggacaag tagtctctca tgatattttc ccaagtgtga
    62161 tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa gatcaagacg
    62221 aagaaacgat gcaacgctat gctcagctcg tacctatgtt ccaagaaatg ggtgaattgc
    62281 accgaatcct tgaaacgatg cgaatcaaac gtggcgcgat ttcttttgag gatcgcgaag
    62341 cgaaaatcct tgttgatgct gaaggacacc caaatgacat ccagctaaga agcagaggaa
    62401 ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa tgaaacggta gctgaacatt
    62461 ataataaatt aaatctgccg tttatttacc gaatacatga acagccaaaa gaagaaaaaa
    62521 tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga acgaaaggaa
    62581 cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa ccagaagctg
    62641 cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca gaagacaatt
    62701 ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct attcgacgct
    62761 atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa agtgaaagca
    62821 atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct aagatggaac
    62881 gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa ttcatgatgg
    62941 acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc ggcctgttta
    63001 tcgaactgcc gaatacagta gagggattga tccatatcaa tgaattgaaa caggactact
    63061 tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg acttttaaaa
    63121 tcggacagaa agtgcgagta aaagtagtca aagctgatcc agatgaacga gcagtagatt
    63181 ttgaacttgt ttccgcagaa gaggttacag cgttggaacg gccaaaaaca cgaaatggaa
    63241 aatcaggaaa acgcgaagga aaacaagcag gaaaacgccc agaaagaaat ggcaagaaga
    63301 acaaacgata tagcgatagt aagcaaacgt tcggtaagaa aaaagggaag aagccatttt
    63361 acaaaggtgt taaaaaaaag aaaaagaaaa aataaaatga gttggaggaa acagctatgc
    63421 caaaaggcga gggaaaatta attgcacaaa acaagaaagc tcgccatgat tattcgatca
    63481 tcgacacgat ggaagcaggg atggtcttgc aaggaaccga gatcaagtcg atacgaaaca
    63541 gccggatcaa tctaaaagat ggatttattc gcgtccgcaa cggagaagct ttcttgcata
    63601 atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg ttgcgcacga
    63661 gaaagttatt attacacaaa aaacaaatca tccggcttga aaatgaattg aaaaatactg
    63721 gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag gtattgattg
    63781 gtctggcgaa agggaaaaaa tcttatgata aacgggaaga tttgaaacga aaagatatcg
    63841 atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat cctttttcgc
    63901 atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa tctgataaaa
    63961 catgattatc ataggttttt tcttagatca ggagcaccgt gtataaagaa aaattcgctt
    64021 cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga attttctgtt
    64081 tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga tacgcacatg
    64141 aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga acgctcgcta
    64201 acgttccaca ttggaccaat ttggtttgat ggtacagttt gtataatggt gttactgacc
    64261 tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt gaaaccaaca
    64321 ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt aacagataat
    64381 ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt tttatttgtt
    64441 tttgttgcaa acaacatcgg tttagtaaca aaaatcgttt tacctaacga aacaacactt
    64501 tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat aatgatctta
    64561 cttactcact tatttggggt gaaaaagtta ggcttcaaag gatatttcgt aaattcgttc
    64621 ttgaaaccat atagttttat gtttccgatg aaactgattg aagaatttac aaacttatta
    64681 acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct aaccttgatt
    64741 gcaaaaatgg taataagctt aggctggttc tcattgccgt tagcaattcc attagagatg
    64801 gtctggatcg cattctcctt gtttatcgga agcatccaag catttgtctt cgttacttta
    64861 tctatggttt atatgagcca taagattgaa gtggaagaat aatattcgtt ttaaaaacaa
    64921 actactattt atctaattta ggaggaaaac aattatgaat tatatcgcag cagcaatcgc
    64981 aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct ctaaaacaat
    65041 cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa tgtttatcgg
    65101 ggtagctcta gtcgaagctg ttcctatctt aggggtagtt gtcgccttga ttctagtttt
    65161 tggtgtttaa ttgaatcagt attaaggcag aaacaatttg gtttgttact gcttttttac
    65221 atgaaaacta gaagtacttc aaagaaagga aggctcgcct atgctgaatc aattggcaat
    65281 tgcagaagtt ggcaatgcga tgttaggaaa tgtcatcgtt gtcagcggct catttttgat
    65341 actactggct ctcttgaagc attttgcttg gggaccaatc agcgatattt tgaaaaaacg
    65401 tgaagacaag atcgccaatg atttggattc tgcagaacaa tctcgcatca actcagcgaa
    65461 aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag ctgatatcat
    65521 caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg atgctcaaga
    65581 agaagcagct cgtctaaaaa gtaaagccca agctgatatc actgtagaac gtgattcagc
    65641 gctgaactct gtaaaagacg atgttgcaga actatctctt caaatcgcgg aaaaaatctt
    65701 gaacaaagaa ttatctccag aaatgcacga atcattaatc aatcaatata tcgaaggtct
    65761 aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg aaagctttat
    65821 ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc gtaagcttac
    65881 gtcaaatcta tgaacaagta cctggattag gtaatatgtt aagtgatgtt cgtctagaac
    65941 ctcatgaaaa acgagaaatc atggacaagc ttgttagtgg ctatgatgga atcgttaaaa
    66001 actttttaga agttgtttat gcgtataacc gaatggatga tcttttgttc atgatcgaag
    66061 aatatgaacg tcgatatgat gaacatcaag gtttgctgtt gggaagtgtc acaacagcag
    66121 ttccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa acaatgaact
    66181 atcaatcagt cgaattaaaa caaatcgttg attcttctat tattggtggc gcgattgttg
    66241 aagctaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaat atgcgtaatc
    66301 aattaaatag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc agaagaaatt
    66361 agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt cgaagaaatc
    66421 ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga aaacgcgatg
    66481 agtggtgagt tgcttgaatt ttcaaatggc tcatacggaa tggcgcaaaa cttagaatca
    66541 aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg agataaagta
    66601 aaacgaacag gaaagatcat ggaagttcca gttggagagg ccttgatcgg acgggtagtt
    66661 aatccgctag gtcagccaat cgacggacta ggtgaaatcg ttactgacaa agctcgtcca
    66721 gtagaagcaa tggcaccagg cgttatgcaa cgtaaatctg ttaacgaacc aatgcaaaca
    66781 ggtctaaaag caatcgacgc tcttgtacca atcggacgtg gccaacgtga attagttatc
    66841 ggtgaccgta aaacagggaa aacttctatt gcgatcgata cgatcatcaa ccaaaaaggt
    66901 caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt tcgtacacaa
    66961 gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa tgccggtgcg
    67021 tctcaaccag cgccgttgct ttatatcgca ccatatgctg gtactgcaat gggtgaagag
    67081 ttcatgtaca acggtaaaca tgtcttgatc atttttgatg atctttcaaa acaagctgtt
    67141 gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc ttatccaggg
    67201 gatgttttct acttacactc acgcttattg gaacgtgcag caaaattaag tgatgattta
    67261 ggtggcggtt caatgactgc cttaccattt gttgaaacac aagctggaga tatctctgct
    67321 tatatcccaa caaacgttat ttcgatcact gatggacaaa tcttcttaga aagcgacttg
    67381 ttctacgcag gtactcgtcc agcggttgat gccggactat ctgtatctcg tgtagggggt
    67441 tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga cttagcaagt
    67501 taccgtgaat tagaagcatt tacacaattc ggttctgact tggatgcagc aacacaagca
    67561 aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaattgca tgcaccgcta
    67621 gctgttgaaa aacaagtagt gattttgtat gctttgacac atggattctt agatagtatc
    67681 cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga taccaatcat
    67741 tcagatatct ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga agcgttgaac
    67801 agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa ttcctctaca
    67861 gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg gtgcttcatt
    67921 aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca cgcgggcaat
    67981 gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga agtttcaggt
    68041 ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt taaatgatat
    68101 tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca gccgtcctgt
    68161 taagaaaact ggttatatcg tcatcactgc agatggcggt ttagtcggtg gatataacag
    68221 ctcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac ccgatgatta
    68281 cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg gtatcaatct
    68341 agcatatgag ctgcgcaatc tatctgatca gccaagcttt gatgaagtcc gcaaaatcgt
    68401 aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg tatgttataa
    68461 ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc caatctcaga
    68521 cttagatcca gaagaagcaa ctactttcga acaagaatat atttttgaac cttcaaaaga
    68581 agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg gtgcgatcgt
    68641 tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag caactgataa
    68701 tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac aaggggctat
    68761 tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt attgagattg
    68821 gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt gtcgacgtgg
    68881 aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt tataaaaatg
    68941 acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac ggagtgatcc
    69001 ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc attgatacag
    69061 gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc aacgtactag
    69121 gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc gaaattcata
    69181 aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa acagggatca
    69241 aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta ttcggtggtg
    69301 ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc caagaacatg
    69361 gtggtatttc tgtatttact ggtgtaggtg aacgtactcg tgaaggtaat gacttgtatt
    69421 atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga caaatgaacg
    69481 aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg gaatatttcc
    69541 gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt ttcactcaag
    69601 ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt tatcaaccaa
    69661 cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa aaaggttcga
    69721 tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg gcgccggcaa
    69781 cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca gaacaaggga
    69841 tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct gaaatcgtgg
    69901 gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga tatcgtgaat
    69961 tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa aaagtgttag
    70021 tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg gctgaacaat
    70081 ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga ttccgcgaaa
    70141 tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt ggccgaatcg
    70201 aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc catggatcaa
    70261 tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca tgcagctatc
    70321 gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc accaatcatc
    70381 gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac ccacgtcgat
    70441 tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc aatcgttgct
    70501 gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc aaaacaacgc
    70561 gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt gaaacgggca
    70621 actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc ataaaatgag
    70681 atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga agtggtcagc
    70741 ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatctcagct
    70801 tatttttcga agaagctact tctggaacac cgtatatcag aggttgtaaa atcagcgttt
    70861 tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc gttttttgag
    70921 ctaataccga gggtcagatg attgaacacc gtttattt
//
LOCUS       DACCPY010000003        61753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCPY010000003 DACCPY010000000
VERSION     DACCPY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.003"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..552)
                     /locus_tag="IVO69_000141"
     CDS             complement(<1..552)
                     /locus_tag="IVO69_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7398952.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYFVG"
     gene            complement(568..1047)
                     /locus_tag="IVO69_000142"
     CDS             complement(568..1047)
                     /locus_tag="IVO69_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7398953.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1053..2077)
                     /locus_tag="IVO69_000143"
                     /pseudo
     CDS             complement(1053..2077)
                     /locus_tag="IVO69_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2094..3200)
                     /locus_tag="IVO69_000144"
     CDS             complement(2094..3200)
                     /locus_tag="IVO69_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7398954.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="IVO69_000145"
                     /gene_synonym="adhC"
     CDS             complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="IVO69_000145"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7398955.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="IVO69_000146"
     CDS             complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="IVO69_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7398956.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="IVO69_000147"
     CDS             complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="IVO69_000147"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7398957.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8171..8869
                     /locus_tag="IVO69_000148"
     CDS             8171..8869
                     /locus_tag="IVO69_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7398958.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9037..11676)
                     /locus_tag="IVO69_000149"
     CDS             complement(9037..11676)
                     /locus_tag="IVO69_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7398959.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12295..13326)
                     /gene="queA"
                     /locus_tag="IVO69_000150"
     CDS             complement(12295..13326)
                     /gene="queA"
                     /locus_tag="IVO69_000150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7398960.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13519..14694
                     /locus_tag="IVO69_000151"
     CDS             13519..14694
                     /locus_tag="IVO69_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7398961.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            14698..15339
                     /locus_tag="IVO69_000152"
     CDS             14698..15339
                     /locus_tag="IVO69_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7398962.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            15336..16253
                     /locus_tag="IVO69_000153"
     CDS             15336..16253
                     /locus_tag="IVO69_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7398963.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            16250..16921
                     /locus_tag="IVO69_000154"
     CDS             16250..16921
                     /locus_tag="IVO69_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7398964.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(17462..17950)
                     /locus_tag="IVO69_000155"
     CDS             complement(17462..17950)
                     /locus_tag="IVO69_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7398965.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(18151..18417)
                     /locus_tag="IVO69_000156"
     CDS             complement(18151..18417)
                     /locus_tag="IVO69_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7398966.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(18417..18680)
                     /locus_tag="IVO69_000157"
     CDS             complement(18417..18680)
                     /locus_tag="IVO69_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7398967.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(18673..18945)
                     /locus_tag="IVO69_000158"
     CDS             complement(18673..18945)
                     /locus_tag="IVO69_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398968.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            19156..19341
                     /locus_tag="IVO69_000159"
     CDS             19156..19341
                     /locus_tag="IVO69_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7398969.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(19483..21306)
                     /locus_tag="IVO69_000160"
     CDS             complement(19483..21306)
                     /locus_tag="IVO69_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7398970.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(21904..24609)
                     /locus_tag="IVO69_000161"
     CDS             complement(21904..24609)
                     /locus_tag="IVO69_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7398971.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(24915..25862)
                     /locus_tag="IVO69_000162"
     CDS             complement(24915..25862)
                     /locus_tag="IVO69_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7398972.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            26052..26321
                     /gene="rpsN"
                     /locus_tag="IVO69_000163"
     CDS             26052..26321
                     /gene="rpsN"
                     /locus_tag="IVO69_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7398973.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(26497..26859)
                     /locus_tag="IVO69_000164"
     CDS             complement(26497..26859)
                     /locus_tag="IVO69_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7398974.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(26870..27991)
                     /locus_tag="IVO69_000165"
     CDS             complement(26870..27991)
                     /locus_tag="IVO69_000165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7398975.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(28007..28357)
                     /locus_tag="IVO69_000166"
     CDS             complement(28007..28357)
                     /locus_tag="IVO69_000166"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7398976.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(28487..29998)
                     /locus_tag="IVO69_000167"
     CDS             complement(28487..29998)
                     /locus_tag="IVO69_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7398977.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(30320..31672)
                     /locus_tag="IVO69_000168"
     CDS             complement(30320..31672)
                     /locus_tag="IVO69_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7398978.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(31825..32901)
                     /locus_tag="IVO69_000169"
     CDS             complement(31825..32901)
                     /locus_tag="IVO69_000169"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7398979.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            33097..34842
                     /locus_tag="IVO69_000170"
     CDS             33097..34842
                     /locus_tag="IVO69_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7398980.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(35122..35559)
                     /locus_tag="IVO69_000171"
     CDS             complement(35122..35559)
                     /locus_tag="IVO69_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7398981.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(35543..36916)
                     /locus_tag="IVO69_000172"
     CDS             complement(35543..36916)
                     /locus_tag="IVO69_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7398982.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(37238..37726)
                     /locus_tag="IVO69_000173"
     CDS             complement(37238..37726)
                     /locus_tag="IVO69_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7398983.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(37748..38350)
                     /locus_tag="IVO69_000174"
     CDS             complement(37748..38350)
                     /locus_tag="IVO69_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7398984.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(38854..39483)
                     /gene="udk"
                     /locus_tag="IVO69_000175"
     CDS             complement(38854..39483)
                     /gene="udk"
                     /locus_tag="IVO69_000175"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7398985.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(39567..40262)
                     /locus_tag="IVO69_000176"
     CDS             complement(39567..40262)
                     /locus_tag="IVO69_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7398986.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(40275..40991)
                     /locus_tag="IVO69_000177"
     CDS             complement(40275..40991)
                     /locus_tag="IVO69_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7398987.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            41321..41926
                     /locus_tag="IVO69_000178"
     CDS             41321..41926
                     /locus_tag="IVO69_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7398988.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            42251..42574
                     /locus_tag="IVO69_000179"
     CDS             42251..42574
                     /locus_tag="IVO69_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7398989.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            42756..44126
                     /locus_tag="IVO69_000180"
     CDS             42756..44126
                     /locus_tag="IVO69_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7398990.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(44256..45557)
                     /locus_tag="IVO69_000181"
     CDS             complement(44256..45557)
                     /locus_tag="IVO69_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7398991.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(45787..47778)
                     /locus_tag="IVO69_000182"
     CDS             complement(45787..47778)
                     /locus_tag="IVO69_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7398992.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(47922..48188)
                     /locus_tag="IVO69_000183"
     CDS             complement(47922..48188)
                     /locus_tag="IVO69_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7398993.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(48698..50824)
                     /gene="pnp"
                     /locus_tag="IVO69_000184"
     CDS             complement(48698..50824)
                     /gene="pnp"
                     /locus_tag="IVO69_000184"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7398994.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(51012..51281)
                     /gene="rpsO"
                     /locus_tag="IVO69_000185"
     CDS             complement(51012..51281)
                     /gene="rpsO"
                     /locus_tag="IVO69_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7398995.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            51532..52095
                     /locus_tag="IVO69_000186"
     CDS             51532..52095
                     /locus_tag="IVO69_000186"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7398996.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(52348..52869)
                     /locus_tag="IVO69_000187"
     CDS             complement(52348..52869)
                     /locus_tag="IVO69_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7398997.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(52872..53714)
                     /locus_tag="IVO69_000188"
     CDS             complement(52872..53714)
                     /locus_tag="IVO69_000188"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7398998.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(53707..54432)
                     /locus_tag="IVO69_000189"
     CDS             complement(53707..54432)
                     /locus_tag="IVO69_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7398999.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            54608..54904
                     /locus_tag="IVO69_000190"
     CDS             54608..54904
                     /locus_tag="IVO69_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399000.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            55472..57064
                     /locus_tag="IVO69_000191"
     CDS             55472..57064
                     /locus_tag="IVO69_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7399001.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            57077..57817
                     /locus_tag="IVO69_000192"
     CDS             57077..57817
                     /locus_tag="IVO69_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7399002.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            57985..59334
                     /locus_tag="IVO69_000193"
     CDS             57985..59334
                     /locus_tag="IVO69_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399003.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(59462..60052)
                     /locus_tag="IVO69_000194"
     CDS             complement(59462..60052)
                     /locus_tag="IVO69_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7399004.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            60199..61551
                     /gene="brnQ"
                     /locus_tag="IVO69_000195"
     CDS             60199..61551
                     /gene="brnQ"
                     /locus_tag="IVO69_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7399005.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT        17592 a        12973 c        11487 g        19701 t
ORIGIN      
        1 gcctacgaaa taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
       61 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
      121 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
      181 accaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
      241 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
      301 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
      361 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
      421 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
      481 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
      541 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
      601 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
      661 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
      721 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
      781 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
      841 tacggctaaa cgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
      901 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
      961 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     1021 taataccttg tttaaaatca ttttcattac agctacgctc cttgctcttg gatcttttga
     1081 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     1141 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     1201 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     1261 gcttcttcaa tggctttttc ctgttcatca gttagctgtt ctttgatggc atcattcatg
     1321 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc taccccgcca
     1381 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     1441 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     1501 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     1561 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     1621 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     1681 atcaccttaa agaaatgttc ttcatcattg aatagataag gaacggaaaa aatcgaataa
     1741 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     1801 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     1861 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     1921 actggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttacg tctgcatctg
     1981 cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga cctaggctaa
     2041 gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt cttttaacaa
     2101 taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata aggcattgct
     2161 gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg
     2221 ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc acgataagcg
     2281 acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat accgtaagca
     2341 aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa cgtatgccat
     2401 aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag tgtttcgatc
     2461 aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat ctctggaata
     2521 gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt atgatttcct
     2581 tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta tttgatatgg
     2641 atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag tttcccgatt
     2701 tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt cttattttcc
     2761 ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc attcatgacc
     2821 cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag
     2881 taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc gccttcttcc
     2941 atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt tgtcatctgg
     3001 taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac tgtccagctt
     3061 ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc gtctttgatc
     3121 gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt aggtacaaaa
     3181 atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt ttatatcctt
     3241 aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac tgtctaaaat
     3301 tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa aaaaagaact
     3361 tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt ttctacgctt
     3421 tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga gatcaatggt
     3481 tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat acggtctacg
     3541 gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt gatgccgaca
     3601 gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc tgttgtttcg
     3661 cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg gaaatagtca
     3721 tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat aatatgcggt
     3781 aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat tttatgtgcg
     3841 actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat tgtagaagcg
     3901 ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc taaatagtca
     3961 aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc catgactgaa
     4021 acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt gacagatgct
     4081 ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa tgcataatct
     4141 gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg cgttacttca
     4201 gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg tttgtcgatc
     4261 ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa agatgtgtct
     4321 gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc gcctccaaga
     4381 gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta tacggtattt
     4441 gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt tttacgtcgt
     4501 gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg gtcacaaacg
     4561 atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt cttttcaaag
     4621 aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc tacagttttg
     4681 acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc ataagaaccg
     4741 catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc gccttctgca
     4801 gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt gacatgcaac
     4861 tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt caacatacct
     4921 tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag aactggagaa
     4981 agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc aatcagcatt
     5041 ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc agaatgtcca
     5101 acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc taatttttgc
     5161 aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt tactgctgca
     5221 tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc attatcaaat
     5281 gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt ttcaatataa
     5341 gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta agcagacttg
     5401 accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt cattagcata
     5461 gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg agctccggca
     5521 gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg ggcacttgga
     5581 tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt
     5641 gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg ttcagcaatt
     5701 tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc atgtttgata
     5761 ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc gtaaacaccg
     5821 cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa agctgccatt
     5881 gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat ttctttcaat
     5941 gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat tgtcttcgtt
     6001 ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg ttatttgtta
     6061 aatcattcac atgtatatca tacaatattt atttttctat gtaaacactt ttttgtgatt
     6121 tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg tatagaaaaa
     6181 tatataaagt tatttattat tatttatatg acacacccga ttattcaata ttgagcaata
     6241 tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac atctttctgc
     6301 cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt ctgtctcttc
     6361 cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt ctgttgtttc
     6421 aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt tgattgctgc
     6481 acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc
     6541 gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac ctggtttcat
     6601 gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa
     6661 ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca tttttttcac
     6721 ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata acactagttt
     6781 tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt tattgaaccc
     6841 gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga ttgcttgacg
     6901 tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca aacgaatccc
     6961 aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt ttttacatgt
     7021 ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg catttttcac
     7081 tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag taggcaagac
     7141 acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag cagcaccgac
     7201 acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt ctaatacacg
     7261 attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac cagggaaatc
     7321 catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg ctccttggat
     7381 gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc gttctgccca
     7441 tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga atggtggaca
     7501 ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga ttgctttttc
     7561 tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc
     7621 gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg
     7681 ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt cttcaccagg
     7741 gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt ctttcaactc
     7801 ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg
     7861 gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt tttctttctt
     7921 gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct atataaaata
     7981 tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc cctcggcttc
     8041 cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca aaaaaaagaa
     8101 aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta aagaaaagga
     8161 gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg
     8221 acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta ttgctactat
     8281 tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg gatcagcgag
     8341 cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg gaattatttc
     8401 cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg atattttacg
     8461 tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca aaggtttgtt
     8521 tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat tatggggttt
     8581 gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct attttattta
     8641 ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga tcactgcaag
     8701 tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt catttatcgg
     8761 ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca gcccatacat
     8821 ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa agagagtgtg
     8881 acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta aaagaaacat
     8941 tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct ttttgtatag
     9001 ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt ctgcttccgg
     9061 taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata aaatccctac
     9121 caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg taaatggcag
     9181 caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca aagctttatt
     9241 ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca catgcagaat
     9301 cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac ttgcgtctac
     9361 ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg ttacgatggt
     9421 gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga tcccttcatt
     9481 tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa
     9541 tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg taaatggtgc
     9601 tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat tacagcttag
     9661 caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac tgacagcgtc
     9721 tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt gcgccacatc
     9781 tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg gtgcatcatt
     9841 cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt tgacgatccg
     9901 gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac gcgcatctaa
     9961 ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg gatcattcaa
    10021 gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag tgatcatgac
    10081 tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg gacgtggtgg
    10141 atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt cagaagaaat
    10201 atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca gtgcccgttt
    10261 accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa agcgcggagc
    10321 aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt ggtgaactgt
    10381 tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat atttttcttc
    10441 taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta cttctgttgg
    10501 attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc
    10561 agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc
    10621 atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt
    10681 gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact gtcggatgat
    10741 cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa caataacagt
    10801 caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag
    10861 tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta aagcgatcat
    10921 actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt
    10981 ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg cagtgacgat
    11041 cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa gttggtcacc
    11101 taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt cactttctcc
    11161 agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg catcggcaga
    11221 aatagcatca ccggactcta aaacaagaat atcacccact accagttgct ctgactcaac
    11281 agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat cttgcaagga
    11341 ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt tgatcacaac
    11401 gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa atgcggtata
    11461 aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca agcttcgaaa
    11521 gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt
    11581 tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt ctgttacctt
    11641 ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt aaaatcttcg
    11701 tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc aaataaaggg
    11761 cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg caaacgctac
    11821 caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga cagaagaatt
    11881 tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca cccagacatg
    11941 cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc
    12001 cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg aaaattgaat
    12061 aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat
    12121 gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat ttctgtttat
    12181 ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt tttctttgtc
    12241 ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat aaccttattt
    12301 cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg catgctggta
    12361 agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta aagtcgattt
    12421 tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc caggcgtaat
    12481 gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt
    12541 ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat ttttacgtac
    12601 ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat gcatatggtg
    12661 ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat gtagtgtcag
    12721 atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa
    12781 tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt gatagcgatc
    12841 tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca atgattcaag
    12901 aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt gttccaattc
    12961 ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc caactttggc
    13021 acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg ttagaagaag
    13081 gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag gcagaactcg
    13141 tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt catggaaatg
    13201 tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat gatcgcggtc
    13261 ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat cttctgttgt
    13321 taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt ttttcgttct
    13381 gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa aaaaactacc
    13441 ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt cattgaaaat
    13501 agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta taaaggtgga
    13561 aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat ttgttttatc
    13621 ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat gactgatcca
    13681 acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc agtggaattg
    13741 cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat gacgatccgt
    13801 gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg gaataaaaca
    13861 gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg ctacccacat
    13921 gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa
    13981 gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg tgattccttg
    14041 caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat
    14101 gacatggatg aagcgattaa actagcaagc cgtatcgcca tcatgagtga aggccgtgtg
    14161 atccaatttg atacaccaga aaatatttta cgtcatcctg caaacgattt cgtagaagag
    14221 ttgattggag aagatcgcct actacaagcc aagccaaatg cgacaagagt cggcgaagtt
    14281 atgctaaata cagcaatcac gattacaccg gaaaaatcat tacaagaagc aatcaaactg
    14341 atgagggaaa aacgtgtaga tacgttgtta gttaccgata attcgaatgt attaaaagga
    14401 tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag tatccagtgt cggcgacatt
    14461 ttgaataaac atgtttttta cgtaaaagaa tccgcctatc ttcgtgatac cttgcaacgc
    14521 attttaaaac gcgggctgaa atacgtgccg gtcgttgatg aacagaacaa agtcgtaggt
    14581 atcctgactc gtgcatcatt agtggatatc gtctacgatg tgatttgggg agaagatccc
    14641 gcaactactg atgttgcaga gtcagctact cctgaagaat ctaaggaggt ctgacctatg
    14701 cagcagtttt ttcatcagca cggttcagaa attctctcaa aaggttttga acatctttat
    14761 atttcaggac ttgctttgat cattggtatc atcattgctg taccgcttgg tgtcttactg
    14821 acacgttttc caaaagctgc cacgattgtg attggcttaa ccagtgcctt gcagacaatc
    14881 ccttctcttg ctttattggc attgatgatc ccgctatttg gcgtaggtaa attacctgcg
    14941 attatcgcac tctttattta ttcattattg ccgattttac gtaatacgta cattgggatg
    15001 aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca tggggatgac tgagttccaa
    15061 tccatttttt ccgttgaatt gcccattgct atgccaacga tcatggctgg tattcgttta
    15121 gctgccgttt acgtgattgc atgggctaca cttgcctctt atatcggcgc tggtggttta
    15181 ggcgatttga tttttagtgg actgaataat taccaaccag atctcatttt ggcaggtacg
    15241 atccctgtaa caattttagc actattggct gattggcttc taggtctctt ggaacaccgg
    15301 ctgacaccga tcgcacttag agaggagaat gaagaatgaa acgtatacta aaaatgacca
    15361 tggttctttt ctctcttctt atacttgcga gttgttcctt ccctggtctt gccagcaata
    15421 cagatgaaga caccatttcg atcacaggtg gaatcacttc agaagcacag atcttagcaa
    15481 gtctagttgc cggaatggtt gaacattata cagataaaaa taccgcaatc atcaacaatc
    15541 tggctactac aactatcaac catcaagcga tgatgaacgg agatgcttca atttcagcgg
    15601 ctcgctacac aggaacagat ttaacgacta cattgaacct tcctcctgaa aaagatccga
    15661 aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag atacggtcaa acgtggttcc
    15721 cgtcctatgg ttttgaaaat acgtatgtct ttcttgttcg aaaagacacc gcacaaaaat
    15781 accatctatc taaagtcagc gatctgaaaa atgtagcgga tgaattagta gctggtgtag
    15841 atacttcttg gatcaatcgt aaaggagacg gttatgacgg cttccaagaa acttatggct
    15901 tctccttcaa ttcgatatta cctatgcaga ttggacttgt ttatgacgca gtcgaagcag
    15961 gaaaaatgga tattgttctt ggctactcta cggatggacg gatcgccagt tatgatctag
    16021 tcatgttgga agatgatcgt caattcttcc caccatatga tgctgctcca gttgtagaca
    16081 acaagatatt agctgatacg cctcatttaa aagaagcctt aaataagtta ggtcatacca
    16141 tcagtacgga aaaaatgcag gaattgaact acgaagcaga taataatcta gtggaaccat
    16201 cagtagtagc acaacgtttc ttacaagaaa accactattt tgaagggaag tgaagcaatt
    16261 ggataaaatg aatgtctttc aacaattttt ctattatttc caggaaaatg gctcctatat
    16321 ctttgcacaa tttatccgtc acttcctgat ttctatctat ggtgtcctct ttgcagcagt
    16381 tgtcgggatt ccggttggga tcatgatttc taggagacgt aaattagcaa actgggtgat
    16441 tcgtttggcg aatatcatcc agacgatccc ttctctagcc atgatctcca ttttaatcat
    16501 tggattaggc ctgggagtca atgtcgtgat tgttactgta tttttgtatt ccctcttacc
    16561 gatcatcaag aatacgtata ccgggatgat ccaagtagac aaaaacatcc ttgatgtcgg
    16621 caaaggcatg gggatgactg ctagacaacg actattcatg gtagagttac cactctctgt
    16681 ttctgttatc atggcaggca taagaaacgc gttagttgtt gccatcggga taaccgcgat
    16741 cggtgcattc gtcggtgctg gtggtcttgg agatatcatt atccgaggaa ccaatgcaac
    16801 agagggcaca tcgattattt tagcaggtgc attgccaact gcattaatgg cgatcatcac
    16861 ggactggctt ttaggtattt tggaaagacg gctagatcca gccagccgta catcacgctg
    16921 atgttattgt aaaaaacaaa agcttctgcg accatttgtt ggtcgcaaag gcttttttag
    16981 ttctgtcatt tttaaggcaa taatccactc tgattcttcc ctccttatgg gcaaagagtt
    17041 gcttttctta gctatttatc cattccgtat actccaactt catactgcca acgggttcat
    17101 atctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc aatccatttt
    17161 cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct gcttgttctt
    17221 tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc ttttccatca
    17281 gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac tggtgactgc cgtatcggta
    17341 acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc taaaaacata
    17401 tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg gtacacgatt
    17461 cttatggact gcatccgtcg caactagact tggcgatacg atgatccttc accagaagag
    17521 cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg gcgtaaaaga
    17581 tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa agcgccacga
    17641 ctttaacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg gatacccgct
    17701 ctgctaaagg aacgacactg gtattgtatt tcgtatctgt atcttttcca aaagaccatg
    17761 ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt gcttttttca
    17821 acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc tcactttggc
    17881 gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt ttactgattt
    17941 tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc attcctaaac
    18001 tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat catgcaataa
    18061 atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag actctttgtt
    18121 aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg cagaataaat
    18181 ttctacaact ttattttcct catcaacttt gtaaaccact cgatgctgtc tattcagcct
    18241 gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg tgggcatata
    18301 tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat tcgtttgctt
    18361 gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt tactcatcaa
    18421 agagcttccc aatcaatatc atcgatattc gtaaagctac tgtcgtcttt ttcgcggtct
    18481 cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat cgctctccag
    18541 tcttccagcc caatcaatac agcattgttt ccactacgat cactaataat ctcgatttca
    18601 gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc agtgctagga
    18661 ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat agcgtacttg
    18721 attaagtacg tcaaaaaaat acaataaagc acttaaattc agcaaacttt tcctcttttt
    18781 tacatagaga aaaagacccc tttttaggga ggatctttgt ccacgctcac ttcttactcc
    18841 tttacgacta gcggtagcca aacgtaaagt aattggtaac aacgtatcat ctttagcaaa
    18901 ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa tacacgaagg ttcctcgttg
    18961 cagtgtgact tgattgatat cttgaataac ctgaggatac gcacgaaaga tttgtaacgg
    19021 ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc tcaaaaatga ttgcctcttt
    19081 cgtctcctgt cattcatgtt cactatcgat ttgatacttg cttagaccgt tgggtttgtg
    19141 aatcaccaat taaaaatggc actgagttat ggtaacaaaa aatggtcact tgacgaggct
    19201 gagattccta cttatgaaga cacatggcac ttaagagtcg aggtaaagga agaagctgaa
    19261 aatataggag gctgtacatt atttggcaaa ggattcggaa attatgatca agacatcaaa
    19321 atcaaaatgt tttacagcta aataggaaga ataataacat ttcatacgct gttcccatct
    19381 ttaatggctt atagagggca cctttagctg agaataagac aaaacagacc ctctagcttc
    19441 aattaagagc tagagggtct gtttttgatt ttaaaacttg gtttatttct tcaaatggta
    19501 gctataggtg ctgacaacga tattttcacg ccaagacaat cgcaatctta accgcaaact
    19561 tgtttggtta tggagaatat tctgccagcg tctgtttggt acaaattgag gaatcaacac
    19621 agtagttgta tagttttgtt tcgctgcatt tttacttacg atatctacat aacggataat
    19681 tggattttca atcgagcgat aagaagagtg gacgattgaa aaacggacat ctgggaaatg
    19741 ctttttaaat tctgcttcga tttctttttc tttttcgaca tcttcgtcta gcgaaacatg
    19801 catcgcaacg acataatcac cgatcgaacg tgcgtaattc aacgcaccga tatttacccg
    19861 tgtcacattc ccgactaata caagaacggt attcccgtca taatcatgta gttttgcatc
    19921 attttctaaa cgaagctgtt ctgctacttt tttataatga tcatggatct tataaaaaat
    19981 gaacataacg attggcataa tgatgaagaa aggccaaatg tcccctaacc gatagacaaa
    20041 caaaatcgca atgattgcat atgagatgaa cgcacccgta atattggcaa tcgatttgct
    20101 taaccatctt tttccttctt ttttccattt gacgaccatc cctgtttgag ataaagcaaa
    20161 tggaatgaac acgccgatag agtataacgg aatcaagcgt tctgttgagc catggaaaat
    20221 aaacagcaat acgatcgaac ctagcgctag tgtgataatc ccattggaat agcctagtcg
    20281 atccccacga tcttgataca tgtgaggcat gaatttatct tttgccagat tgtatgcaag
    20341 aactggaaaa gcagaaaaac cggtgttagc agcaactgct aaaatcagcg ctgtagcaaa
    20401 ttgaaggacg taatacaaaa taccatgtcc aaagacagct ttaccaattt gagacaaaac
    20461 agtgacttct ttttcaggaa cgatcccata ccaatagttg ataaatgtga tccccacgaa
    20521 gaagaaacct aagatcaatg ccatcatcgt caatgtagca gcggcatttt ttgctcttgg
    20581 cttcttaaag aatggtacag cattactaat cgcctccacc ccagttaaag aggaagaacc
    20641 agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc ggcacagccg ctcctggaag
    20701 tgcagtagct tgcaatggca cagcacctgt cacgatatta tataaaccaa caacgatcag
    20761 taacgagatc acaaggataa atgtgtacac cggaaataat aggaacgatg cagattccct
    20821 cagcccacga agattcagca tcatcagtag caaaacgatt gtcacagaaa tagcgacttg
    20881 atgaccgtag agtgctggca ccgctgatgt gatcgcttca gcacctgctg agacagatac
    20941 tgctactgtc aacatgtagt cgatcaataa agagccaccg gaaatcaagc cagcattctt
    21001 tccaagattc tcactgctga cgacatatgc tcccccgcca tgaggataag catggatgat
    21061 ttgacgatat gatagcgtta atgaaatcaa taaaataata acaaaagcgg caatgggtag
    21121 cgaataccag atggctgcag cagacaaagc gactaaaacg acaacgattt gttccgtacc
    21181 atatgcaata gatgataacg catcagacga caataatgcc aaagcagcaa atcgggttaa
    21241 tttgtgctca tcattttcag cagattttaa aggcttacct actaacaacc tttttaagta
    21301 atccaatttc taaactcctt tacacacaat tttacttcaa gttttatagt cgaaaattta
    21361 tagtaaacaa gatgttatag tacgctcact gaccttaaaa gtcaataaac tttaagaaaa
    21421 taattggaaa aaaacagaaa aatatcaagc gctttttcaa acgctttaat acagcaaaaa
    21481 tctaacattt ttttatgggc tttttctttg aattctataa taaaaggata gaacttgact
    21541 tagaaaatca agttctatcc tttttgatat cttaactttt ggaagagaga tgtaaggctg
    21601 ctttctaccc atcattctta ctactcgaag catagcgcag ttgtcacaac ctcttgataa
    21661 acggtgttta aaagcttgca tctgcggtaa taagctcaac caaattaaaa acataagaag
    21721 ctgtttttat ttggtcgttc ttattacttg atgcagagca gcttttttac aacctcttgg
    21781 tacacggtgt tcaatcagct gaccctcggt attagctcaa aaaaacggca aaatatgagg
    21841 aactattttt ccattttttt ctctaatact caggtcaaaa cactgatttc acaacctctt
    21901 tttttaatga aaaatatggc tctcatgcgc cgcttctttg aatttcgtca ttttttcttc
    21961 gatgaacgga tagattgccg ttcctacgat tccaaagaca acaaacaaac cgatcatgat
    22021 ccagattgct ttcgtagcat ttggccaata aattcctcct gcagattccc gtaacaagtt
    22081 taccgcatga gtaaatggca agaacggatt gatcatttgg aagaatttcc ctgagacttg
    22141 gatcggatag ttcccgcctc cgccagagat cgacagtact aagatgataa tagcgatccc
    22201 tttaccgaca ttatcgaata atgcagccaa gacatagacg atcatcatga acgctaaggc
    22261 gatcaagaca gcaaatgaca cgctgtagac cggattttgg acatctactc ctagtaagaa
    22321 aatattccct aatgtaacga tcaagctttg cgcaatcgcc attgtcaaaa atgtcagcat
    22381 acgagcaacg aattgttctc gtttcgtaaa attgtttcgg tctttctttc ctaaatagta
    22441 ttctgtcgta gcaacacttg aaagaagaac agcgccgacc cacagacata gtgcagtata
    22501 aaacggggtg ctagctgaac cgttatttgc gattggataa agcgcattgg tttgtagctc
    22561 aacaggttgt gtaaagaagt cactttcttt tgttgcgtct agtttcaata atttgatgac
    22621 ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg tctgctgctt tttggatacc
    22681 agctttgata tttggccagt cattgttgat caagtccgca gcagcgttca cagctgtttc
    22741 gacttctggc attttttcat ttactgttgt caatgtaccg gtgatttcat tttttagtgc
    22801 tggccaatca ttttggataa aatcagcggc caatcctaat ttttgctgaa tgatcggtaa
    22861 ttcattgttg tataggtctg ccccttcatt gatgccattg acgatggtat ccatatttcc
    22921 attcagcatt gtattggcat catggatttc ttgtccaatt gcaggcattt ctttttgata
    22981 tttttctaaa attgccactg cattcgttac agttgctttc gttgagctga gaagcgaatc
    23041 aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca gtcccaatcg tatcgatgac
    23101 ttggtctagt agagaagtga cagtggaact gatttgacct gtattgatac cagcaatgat
    23161 gccattgata ttattaacaa cggcatcaat ttgatctagc aattcatcaa tagccttcgt
    23221 gtcttcattt tgaacagctg tgttcaaagc agataggcgg ttttgcagat tgcttagcaa
    23281 gggttttaat tgatttagct gatcaataat tggctgtaat ttcgtatttc cagcacttgt
    23341 ttgcaattta gtcaggaact cgaccagttt atcgattgca ttttgttgtt gagatattcc
    23401 gtcaataaag ccgttcaata cattgctgat gtttttcttt tcatcatcag tcagcttacc
    23461 atcttcaata gctgttctta cagtatctat taacgaacct gtcgttgttg ccacttgtcc
    23521 aattgacttc aatgtaacat ctacactgtt tgtgatgctt ggtatcgcat ctttcagctg
    23581 agaggcacct gattttgttt ggtttgctag atcacttgcc tgattttcta aatttttcac
    23641 atctggcaag atatcttgta cttgttggat gatcgtcaag ccttgttttg cttcatcgat
    23701 tccttggttc attgtctctt ggatcgaagc aaaatcgccg tcgatctctg ctagttgctt
    23761 accggcattt tggatctctg ggattttttc ttgcaaatca aggatgattt ttgcttgatc
    23821 tttcaactca ggcattttgt cattcaatgc aacgactttt tcacccatct catctacttt
    23881 agggatgtaa tccacgaatt catttgcttt attcagtttt tccttgattt caggtagttg
    23941 actttgtaat tcgaggactt gttttgtgta gccgtcgatc gtgtccaaat tttcatctgt
    24001 tgataaaatc atatctttaa ctttattgat acttactagg ttcgtatcga tgtcgtaacc
    24061 tatctcattg aatactttca ataaagtcga actggctgtt ttgataaatt catttgtgat
    24121 ttgcgattgg atcgaacttg ctcctttatc ggtgattttc ggtgcaatcg cattgatttt
    24181 ttcatttacc gtatattcga tttttggttt cttgatatca ccactagtaa aactcagcag
    24241 atcttctgag aaatcctttg gcaagtaaat acctgcgtaa tactttccgg aacggactcc
    24301 gtcttctaat tgttcttttg aatcaacgaa ttgccatcct aattgtttat ttttatgaag
    24361 cgattcgatg acttgctccc caatggccac ttctttacct tgaaactctg ctggcttgtc
    24421 tgcactgtaa acagcaattg gcagctctcc agtatttcca tatggatccc ataatgcttt
    24481 gatgttaaac cacgcatata gcgaagggat gatcatgatc gcgatgatca gaagtgttgc
    24541 aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat aatcgaagcg tatttttgat
    24601 tgactgcata gttctccccc ttcttctttt ttcagctgaa ttactttacc atgtatttca
    24661 tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt tggacatatc acggcaattg
    24721 tctcattgta atctcttaca tattgagaaa taattaagga ttacagcctt tactttatca
    24781 tgaaataaaa gggaaagata attttatgcc aaagttttta tctattatac gtatacgcaa
    24841 aaaagctaaa acattttttg tctttgcccc tacaaaaatg tttcagcttt tttcgttttt
    24901 tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct
    24961 tcaactagct gatgtgcttg gatgacatgc ttaagtgaag cagacagatt cattgaaata
    25021 aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct
    25081 agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct
    25141 agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg
    25201 gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat
    25261 tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc
    25321 ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc
    25381 tggatcagac ttgacccgac cgcacctgat gctcctagta tcattacagt atctgtcggc
    25441 tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg
    25501 gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg
    25561 acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca
    25621 gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa
    25681 gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga
    25741 ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga
    25801 gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc
    25861 atggtcacca tctccctttt tctttaagtt tacgtttatg tctttatttt gacaaaaaaa
    25921 atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt
    25981 gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga
    26041 ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga
    26101 ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc
    26161 gtaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgattta atcgatggac
    26221 gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac
    26281 ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg
    26341 taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt
    26401 ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt
    26461 tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc
    26521 ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa
    26581 atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat
    26641 aacggagtct ctttctattc ctgttccagt agctttgatc ccaatgtgag tcggaagctt
    26701 aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc
    26761 tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg
    26821 agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat
    26881 attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt
    26941 gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt
    27001 ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc
    27061 caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc
    27121 agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg
    27181 tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatc aattcagaaa
    27241 ccagctcaag tgctggctgc aatggataaa cttccggtag tgcgtgccca gagggattaa
    27301 gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag
    27361 ccgttgcact attgctgaca tgtacatatc gaggaagttc gggaagtttt ttcagcactt
    27421 ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa
    27481 aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct
    27541 cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat
    27601 gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct gcttgcaacc
    27661 attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt
    27721 aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc
    27781 cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg
    27841 tttcaatagc tccatgaccg tatccgttcg cttttactac tgcaaacaat tcttttcctt
    27901 gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg
    27961 ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa
    28021 taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccataca ttgccttcat
    28081 gaggtgaacg cgtcacaaca ggctgaccat tttcattttt taaaatctcg acatcttgaa
    28141 aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac
    28201 gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat
    28261 cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag
    28321 gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat
    28381 ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa
    28441 caaaaatgaa agtttctctt aaaaaagaga aactttcatt ttatgtttaa tcgttatttg
    28501 aacggattac aaatccgcgt tttttatcat tgtgacgttt accgccatct ttttggctgt
    28561 tattattttt gctataaccg ccttttgatt tgtttccacg ataattaccg ccgtcacgac
    28621 gattacggtt gtttccgcca ccacgtttgc catttttatt gtatcctttt ttctgcattg
    28681 gcaatgggcg ttctggtgtg atcttcactg gcactgcatc agctggatct tttgctgtcg
    28741 tttttagcaa aagtgcaact aaatcttgtg cagaatattc ttctaataat ttatctgctg
    28801 tttgcaggta tttatctaga ccattttctg caagttttgt ttcgatttgt tcaactgcag
    28861 cacctaattg acctttgaat gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt
    28921 ttttcgtcaa gttttcaatc acatgcaagt agtccatttc atttggtgta acgaaagtca
    28981 cagacatacc gccttttccg gcacggcctg tacgtccgat acggtgaacg tagctttctg
    29041 gatcttgagg gatatcatag ttgtacacat gagtgacacc tgaaatatct aatccacgtg
    29101 cagcaacgtc tgtcgctaca aggatatcta aatgaccgct cttaaatgaa cgcaatacgc
    29161 tcattcgttt ttgttgagac aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt
    29221 ctaatccgcg tgccaattcg tctacacggc gttttgtacg tccgaagacg attgttaatt
    29281 caggtgtttg aacatcaaac aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc
    29341 ggacatagta ttgatcgatc aagtcagctg tcatttcttt cgctttgatc ttcacatgat
    29401 gtggattttt catgaattta acgccaatat tcttgatagc tggcggcatc gttgctgaga
    29461 aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat tttttcgata tcttccaaga
    29521 agcccatatt caacatttca tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt
    29581 tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg tgtacccaca acgatatgtg
    29641 gacgatcttt caagccacgg atctgacggc caatatctgc tccgccgtaa acggcttgta
    29701 cacgtacttt tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg atagctaatt
    29761 cacgtgttgg tgcgataact aaaccttgta attcgtgacg atctggatcg attttttcta
    29821 acattggtaa tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt
    29881 cttttccagc taatgctagt ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac
    29941 cagcgcgttc gactgatttc aacaattctg gtgataattc tagttcttta aatttcaaat
    30001 gaatcctcct atatggttgt taactgagga tgtagctttt ttgctacccc gtcgatttgt
    30061 tcggtcaaag tgtattgttt ctttttgaca gtggccacgc acgtttttat ccattcttag
    30121 cccctgtggc accaccgaaa aatcactttg cgaagtttag gcattagaaa agccaaacgg
    30181 gtcgcgttca gctttatcat tattatgaca tctggcttca acaagttgtt tctatggcaa
    30241 aaccagtcca atttctcttc atctttcggc aataggttat tctaacatag tttttcgttt
    30301 tctgcaagtt tacagatttt tatttcattt gctgaagttg ttctaccact tcaaataagc
    30361 ccatgctgtt gctgcctttt agcataatac tatctgttgc tgcaagattt tgtttgatag
    30421 catcaatcaa tttattcttt tctttttcaa aatagaacac aggtagttgg gggtattttt
    30481 ggttcaattc ttcttttagt gcatgcattt gttccccata aaggaaaatc atggcgaatt
    30541 tctcaggatc gatatgttca ctcatttgcg catgcatatt taaagactct ttccctaatt
    30601 ccagcatatc tgctaacaca gcgattctgc gtccttttgt tggcagattt ttaaaagtat
    30661 ctaataccag tcccatggct gttggatttg cattgtagac atcgctcaaa atagctgcac
    30721 catttcctgc cgttagccat tcggttctgt tttgagtgat agaaacatgg gcgagccctt
    30781 ggcgtatctc aggtatggac agtccaaaga agcgcccgat cgtatacgca atcaaagcat
    30841 tcgttacatt ataacttcca ggcagcggga tcgtaaatgt ttctccttct actacaaaat
    30901 cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac atcgccattt ccaataccga
    30961 atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg
    31021 gcacaacgag taatccgtct gatacaagtc cgtcagtgat ttccattttg gctttagcga
    31081 ttccttctcg cgacccaaga ttttctatgt gtgcttcacc gatcattgtg attgctgcag
    31141 cttttggttg agccataagt gaaagttccg tgatttcatt ggcgtgatcc atgcccattt
    31201 ccaaaacgat cttttctgta tcttggggca tgtgcaaaat cgtgtaagga agaccaatat
    31261 tattgttgta atttccttgc gtcttatatg ttttgtattt ttcagacaag acagacgcgg
    31321 ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact tctgcgccta
    31381 ctttttccaa gtaataggtt gccaattgct gcatggcttt caaaggatct gctacaggaa
    31441 taactggaaa attttttgga gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc
    31501 cgtttgtgat cgcttgctgg ataaattcat gtccatctcg cgtaccagca agaggaacga
    31561 ataaacttcc ttcggttatc ttccggctgt caaattcgac attggtaatc tttgtcagtg
    31621 cagagcattc gctgcccacg gcttgtgcaa tttcttgtat tgttagattc attgagatct
    31681 ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc atgactctca
    31741 cttccaagta aatttctcta ttcgtattta gaagctagaa tgaacgcggt tacgcaatca
    31801 ctccagcctc ttctttattt ctctttattc atttttttca aaaaaagatt gacgctgatg
    31861 gtatcgattc attcctaact ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat
    31921 attttcccat aagagtgggt acatactgaa ctccgtaaat cctggcatag agtttaattc
    31981 attcaggaat aattcatttt tatttgtcaa aaagaaatcg caccggctca atccgcttcc
    32041 acctaacatc gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa cttcttctgg
    32101 cacttcggct ggaatctgca tttcgatttt attattgata tattttgctt cataatcata
    32161 gaatgctacg tcttttacga cttcaccagg caaagtcgtc cgaacgtctt catttcctaa
    32221 tacagcaact tcgatttcgc gcgcttcaat tccttgttca acgattgctc gagaatcata
    32281 ctgataggct gttgctaaag cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc
    32341 gacactagaa cccatattcg ccggtttgac aaacatcgga taaagcaaag aaccttcaca
    32401 ttgatcaaat acttttttag gattttcttt ccattgattc ttaagtactg gtacataagg
    32461 aacttgcggc acaccagcag cttgtaaaat atacttggtc atgattttat ccatggcaca
    32521 tgcactggtc aatacgcctg cgccgacata aggcatattc aatgtctcta agaagccttg
    32581 gatcgttcca tcttccccgt ttggcccatg taaaactgga aaaacgatgg ctccttcttc
    32641 tttgatttcg cccggattga tcacttttcc tgtaaagcct tcctctgtct gtccacttgg
    32701 gtcccatgaa agatgcaaga catctttgct ggcaggtttt tctgttagta atggaccttt
    32761 gacccattgt ccttcttttg taataaatac gagttgaact tggtaataat tataataaat
    32821 ggcatttaaa actgaaaatg cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc
    32881 atatagtaaa gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta
    32941 ctctcatatt ttagcacaaa acaaaaataa acgaataact tatttataaa aaaacgagcc
    33001 agcttctaaa agaacgagcg tcatttattt tttgtcagct agattatttg tcccgttttt
    33061 tataaaaagt tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc
    33121 cgcttggcag aaatcattcg agtgttcatc aattacaatg tagtgccaaa ctttgtacag
    33181 caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac taggccccac cttcatcaag
    33241 ataggacaga tgctttctgt tcgagaagat ttgctttctt ctgccttcac tcaaacattc
    33301 aaaacattac aagatagtgt acctagcgac acattttcaa cggtcaaaaa gacgatcgaa
    33361 acagaacttt ctctttcatt atcagatata ttcgatgatt tttcaaaatc gccttttgcc
    33421 tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt
    33481 aagatccaac accctaacat cgccgaagag atccgacttg atcttcaact atttgaacga
    33541 gcgatccctt tgatcaaata cattcctgaa actagcgtag ttgatctaaa aggggtactc
    33601 caagaagtaa aacgttcctt gatcaatgaa atggacttct taaaagaaag tcaaaatggc
    33661 gaacaattct atcaaaagaa caatggctgg aaagagatcc gcagccccaa gatttatgac
    33721 gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat
    33781 ttaatgaacg ccgaaaacaa aactgaaact tttatcactg gaattcaaaa caagcagctg
    33841 aaacaagaag ttgcaaagct tttagttgag aactttatga aacaggtgtt tgatgatggt
    33901 tttttccatg ctgatcctca tccagggaat ctgctattcc atgtactaac aaaagaagaa
    33961 cagacacaag ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg ttcctttgct
    34021 tttcgagcct ccacttctgc agaagaccct gttgcgccat ataccatcaa ctacatcgat
    34081 tttggtatga tggggcatct ttctgcagga cttaggcaaa aactcacaca agccgtttta
    34141 gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa
    34201 gaaggctcgt ttgatgaaag tcggttccac caagaactga caagttttct tgaacagtat
    34261 tatgattctc ctattgatga aatcaacttg caagaagtat tcacacatgt cgtgacgatt
    34321 tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc ttttgctgaa agcatttggg
    34381 acattagaag gggtgatccg cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg
    34441 ccttttgctc aacattattt tctcactcat ctggatgtag aagatacatt gaaacaaagc
    34501 ggattagact tattggaagg gatgaaggct gcgcctaaga tccctcaaca gctccaccat
    34561 ctattagaaa tgtggacttc gggacaagga aaagtcaatt tggaattgaa aaagcaagac
    34621 aaattattga gcaggatcga aagtatgatc aatcggcttg ttttcggtat gattttagct
    34681 gcattgatcg tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc
    34741 gtttccttat tagggatatt tacgtatgct attgccgcat tcgtcattat ttttttggca
    34801 atcgatgcat tgatccaaat gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa
    34861 agaatcagct tcaagaaata agaaggggtt ttacataaat tgtttctcaa atttttgtga
    34921 aaattcggct tatttttcga agaggctact tctgaaacac cgagaataaa aaatgtgaaa
    34981 aagctgttct gcacccagta ataagaacaa tcaaaaaggg aacatggaac acatttatcc
    35041 tgtttccctg ttcccttttt gatgtattcg agcataaata aaataccttc gttacctact
    35101 atcttctgta cttggtaaac cttagtcaat tgcctttttt aacgcttgac gaaaagacat
    35161 atgatctttc tccgtaaagg gttttggacc ctttgtcctt atgccagctt ttcttaactt
    35221 tccactatga taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa attcttttcc
    35281 agagtgatgg aacactcgca cacccttcgg caatacaagt gaagctaaac cataatcttg
    35341 cgtgatcaaa aagtcattct tcttaaccat accgacgatt ttataatccg cactatcagc
    35401 tccgcggtct acgtagacga tttggacatg attagcgtat tctttggtcg tataatggtc
    35461 cacactggtc acgataacca cgtcaagacc ataggattct cctaatttta tgacatcttc
    35521 ttttactgga gaaccatctc catcaatgaa tagcctcatt ctttttcgcc ttcttccata
    35581 aatgataaac agttactaat ccgctcagta aaagaccaac attgatccat tttgccaaga
    35641 taagcagttg tacactttct atcggcactg attcatggtt cagcgtgatc aataatagat
    35701 ggattggctc tcccatttct tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt
    35761 ggatcacact ccctttattt tgagtagtat acttgacaac gatccggcga tccattggaa
    35821 tagctgtatc agtttgataa tattccttat ttagccagct gcctgttact acgtgccaga
    35881 aactaagcag aaagacacct atcgcaaaac catttggaat caagaggacc aataaagaag
    35941 aactcagtcc aagcaattgc atagccaatg gagaccatgt taaacgtttc aaaatccatg
    36001 gtccgactat ttttgctgca atcaagcctg ctgcataagg caaatacata aaaatcgaaa
    36061 gtttttctaa tccgtacagt atgccaacat aaaaactgct aaataaaaaa aggaaattcc
    36121 ctagcattcc atttagaaaa gtaaacagat ttagccagct tggcaacttc cattccttct
    36181 tctggtgtcc taaaacaaac acgaacaaga tgaacagcag caagaaacca taaatagcaa
    36241 gatcaaaaag cctttcatcg aataacaatc gggcatttct caaaagaaac aataagccaa
    36301 agaatatcaa aaataaaaca agctctcgcg tagctaccag atttcttttg atgtctttga
    36361 aatccagttc gtaacgcgga taatgactaa ccgtatggta ggccatgacg taaaaaagtg
    36421 tataaatagt aaagcttgct gcaatctgag tcggtgcagg caactcaatc gcttgataca
    36481 ggagaaatag ccagatagct gcaaataagt actttttccc atccatattg ataaaacctt
    36541 gcgtcttttc gtgaaaattg accgtggtat tagcaggtac aagccatgct gcactcagcc
    36601 ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa tacgcctaat attccaagca
    36661 atgaaccagc accaccaatc agcagagcga tcatcagcaa cgtgtaacta tctagaccaa
    36721 atcgtagact gttgactaaa aatagtccag tcatccgaaa ggcataaaac agcgtaaatg
    36781 gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa aaataaaaga taaggaataa
    36841 aaataaccgt gttacttaag ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt
    36901 tgagtttttc gaacatgaca atggctcctt aaacagtctt ataactaatc tgctcttcga
    36961 ttttagcatt aaaggggaaa taaaccaatt ttacagaata tattcttgta aaaaaaaata
    37021 cagaaagaag cataagcttc tttctgcatt tttacggtac ttgattactc aagcagacaa
    37081 gcccttctcc acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa
    37141 atcaattatc ctgtgcctcc aaaatcaatt aggagctctg taaaccagag tccttctcga
    37201 cccgtcgcat aaatttactt taacacagtt cctatgttta tgcgagagct tttttcattt
    37261 tataatcgac catttgaaat ccttgaccga tatctgaaat aagttcttct gtaatctcaa
    37321 atccttcatg cttataaacc tcgattgctt gatgattccc ctgattcact cgcaaaaaga
    37381 tctctctttc ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact gaagaagtca
    37441 atccttgacc acgtacttta tttagcaaat ataatttact taaataaatt tttccattta
    37501 tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca
    37561 caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat
    37621 ccacttgttt ttgaccaatg atcggcacaa aagcttccgg ccatacttct tgtatgactg
    37681 ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt
    37741 ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca
    37801 cacccaccga ccatggtttc aagaaaatca caatctgcat aaattcccta aaagataatt
    37861 tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa
    37921 caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat
    37981 acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa
    38041 atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc
    38101 caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt
    38161 ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg
    38221 cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa
    38281 agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt
    38341 ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct ccttaggggg
    38401 tgcgacaaaa gagcttcttc aaaaaaataa gtcgaaatct cacacaaatt cgagaaataa
    38461 ttttcgtaag tttcttctta tttattgcat ctgaccactt ctgttacaac ctctcgttaa
    38521 acggtgttca aaagcttgca tctacgataa taagcccaac ccaacgaaaa acgtgaggag
    38581 ctgttttatt tggatgctct tatcgcttga tgcaaagaca gcttttttac aacctctact
    38641 tcacggtgct caatcagctg accttcggta ttagctcaaa aaacggtaaa atataaacag
    38701 ctattttccg tttcttcttt ttaatactta ggtcaagatg ctgttttcac aacctcttgt
    38761 tataaataaa aaaacagttg gctggtgact gttcaaaaag cagaatcctt gcttttcaaa
    38821 cggtcgcagt ccgttcaact gtttcgctgc gcactattta tggtttaaga aactagctac
    38881 ttttgttgta atcaaatcaa tagcaacgtg gttttctccg ccttctggaa caatgatatc
    38941 cgcatagcgc ttcgttggtt cgataaattg atgatacatc ggtttaacaa ctgtcaaata
    39001 ttgttcgatc acggagtcca atgtgcgtcc ccgttcttcc atatcccgtt tgatacgacg
    39061 aatgatacga atgtcatcat ctgtatctac gtatactttg atatccatca agtcacgcag
    39121 acgttcgtct tctaaaatca ggattccttc tagaatgatc acttcttttg gttcctgaat
    39181 gatcgttgcc tgacttcttg tatgcgctac atagtcatac acgggttttt caatggcttt
    39241 atagttcagt aagtcgccta catgctgaat cagcagatca ttatcaaagg caaatgggtg
    39301 atcgtaattt gtgttcaatc gttcttcaaa actcaaatgg ctctgatcct tgtaataaga
    39361 atcttgttca agcatcatga tggaatggtc cgggaagttg ttgaaaatcg cacggctgac
    39421 acttgtcttt ccgcttccgg atccacccgt cacaccaatg ataattggct tgctttttgt
    39481 cattgactaa aacctctttc gtatccgttt tttcattcta atctattata gcgaaaaaac
    39541 acgaaaatgc tatatttttc tgaaaattaa tccttttcaa tttccaataa tgaataaagt
    39601 tcctctaatg aatcgattcg ataagtaggc tggatttctt cgatttctgg taatttattt
    39661 ctattgaacc aaatcgtatc gatccctgcc atatttcctc ctttgatatc agaagtcaaa
    39721 gaatcaccaa tgatcacagt ttgcttttta tcaaaattag gaatgtggga aaagacatga
    39781 tcaaaaaatt ctttcatcgg cttttgcgct ccgacagctt ctgagacaaa gatatccttg
    39841 aagtaatcaa gcattcctgc atctgttaat cgccgatgtt gcgtgtgcga cacaccgttc
    39901 gtcacaatat aaagatcagc atgcggatgg acctttttca ggatttctag acttcctgga
    39961 acaagatcat gaccttggtt caagtagtgg cggtagcgtt tttccatttg atcactatct
    40021 actgttttcc caaattgctg gaaaagtaac ccaaaacgac tgcctattat ttcttctcgt
    40081 gatagtctgc ctttttcaaa ctcctgccat aaatgatgat tcaaagcctt atagctggtt
    40141 tcgatttctg gtgtcaatgt gattccttgt tctgcaaaca aagcacttaa agcattggct
    40201 tcagttaatt gaaaatctaa caatgtatca tctacatcga acaataaagt tttatatttc
    40261 atctttttga cctcctattt aaaataaata cttaactttt tcatctctgc taatgtcaac
    40321 gatcgatact cgcctactcc tagctgttca tctaattcca atggtcccat gcttagccgt
    40381 ttcaatgcgg tcactttctt tccttgggct aagaacatct ttttgacttg atggaatttt
    40441 ccttcttgga tcgtcaataa aaccgtactt tggtgcttgt ctgctttgag tatttttaaa
    40501 tcagccggct tacattgata cccatcagcg aatacgattc cattttcaaa tgctgttacg
    40561 tctgccggtg tcaccctttc gttcacaact acttgataag tctttgaaac ctttttttct
    40621 ggaagtagca tttcatatcc caattgtcca ttcgaagtga tcagcaatag tcctgttgta
    40681 tctctatcta gtcttcccac tggatataat ggttggaaac gatcggcact tgcaatcaga
    40741 tcgattactg tcgtcttttc tggatctgat acagctgtaa cagctttttc aggtttattg
    40801 agaagataat aggactcatt tgtttctaag tgttcacctg ctacttcgat ttgatgcagt
    40861 aaactatcta catttcgatt ttgtcgaaat tcgactgtgc catctatcat cacttgcttc
    40921 aaagcaaaca gccgcttcat ttccttgcgt gtcgtttcta atttttcttc tattaattta
    40981 tctaaacgca tgctgtttcc tttcttatta ctactaatta attatacctt gaatattctt
    41041 gcttgcaaat acatcatcat agttattcct tgacttaatt aacagttcac gttacggtta
    41101 aatagtaaag tgaattggat ttctcataca cagcgtaatg acctggtgga gaaaagttga
    41161 atcctgactt tctaagggga gatgcgcgca tctctccttt tgcgcttttt taagcgccct
    41221 gccttcgatg atgtgcacga gattagcctt tagaattcac ttaaataaaa tctgtccgtt
    41281 accaaagcag acagtcacat tttaaggagg aattttggtt atgtcagtat cattagaagt
    41341 aagtaaaaga gaagtacgtc cccgttcatt aagaaacaaa cttcgtcacg aagggaaagt
    41401 tccagctgtt gtttatgggt ataaagtaga aagtacccct atctcattcg atggtttgac
    41461 actaagcaaa ttgttgcgtg aacatggagc gaacacagtc atcacgatgg acatcgatgg
    41521 acaaaaagtc aatacattgg ttcacaagat ccaaacgaac acttttacca atcatttcga
    41581 acatgcagaa tttctatctg taaacatgtc ggaagaaaca gaagtcgaaa cagaagtcgt
    41641 tcttgttggc gaatcagtgg gtgctaaatc tggtggagtg ttagctcaaa acttgtatac
    41701 agtagtcgta tctgctacac cagacaaatt gcctgaacga attgaagttg acgtgacaaa
    41761 cttggaatta ggtgattcta ttacagtagc cgatcttcca aaaaacgacg attacaaatt
    41821 cgtgaccgac agtgaagagc aaatcgctgc tgtagtcgct caaagtgaag aagaaagcac
    41881 aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact gaaacaatca
    41941 ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt tctaatcgtt
    42001 tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa tcctaattta
    42061 ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact ttgaaaaaaa
    42121 gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct cgttgaagta
    42181 agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg attcgaagga
    42241 ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt cagttggtgt
    42301 cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg acctagatct
    42361 taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa atcgtggaaa
    42421 agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg atttgccgtt
    42481 tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta cgagagaaac
    42541 gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt ggatgtccct
    42601 tattttacag accaggccaa gttgttgatt ctttgactca attcttggct tctttttatg
    42661 gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg ctatatacta
    42721 ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca aaaggaagcc
    42781 ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt tttcagcagt
    42841 tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat gcgctggcag
    42901 cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa ggtttaacag
    42961 ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg ggtctgaaga
    43021 aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta actgtcctaa
    43081 gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa atacttgatc
    43141 aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc agtttcaatt
    43201 tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc tttctgatca
    43261 ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt ggcatgggtg
    43321 tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta ctgtgtctag
    43381 tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc aaatttgaaa
    43441 aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa tcttcgatca
    43501 tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca gatgtcgtag
    43561 ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg atgtcatttg
    43621 gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat ggccgaatct
    43681 tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta gcaggtgcta
    43741 tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca gaaacacgtg
    43801 tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg atcttagcag
    43861 tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc cctacgatcg
    43921 ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca ggaatgtggg
    43981 gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt gccgtgttgc
    44041 tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag gaacatttca
    44101 cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg gctaaaaatg
    44161 aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta gataacaaaa
    44221 aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctatttttct ttccgtggac
    44281 tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa acggaatgat
    44341 gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc aaagcccgca
    44401 agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata acgctggaaa
    44461 tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc cgctatcaat
    44521 gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa aatatcttca
    44581 atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat atgctttccc
    44641 ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt tacgatcggc
    44701 tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt gatcaaatct
    44761 ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg atagcgagta
    44821 tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc ttcgaatact
    44881 tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa ttcaaacacg
    44941 ttttccaaat aatgatattc ttctttggtc aactctcctt tttcataaga attcaatgcg
    45001 actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag acgcacacct
    45061 aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg aaacattact
    45121 ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt attgacgata
    45181 ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac taaaataaat
    45241 cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt accgatcact
    45301 ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa accggttagc
    45361 gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac atgaagagct
    45421 agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg tctgattttc
    45481 actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa aaataaaata
    45541 atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc tttttctaca
    45601 aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg cggcattttt
    45661 cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa cagctttttc
    45721 atgacctctg ataaacggtg ttcaagtagc tgacattcga cattagttca ggtcaaagcg
    45781 ctactttcac aaccgctctt tcaaccatgt tttccgagta tttccaatga taagcggaac
    45841 agtttcatgt tttacttcgc tgtactctag cccaaaccat gtttctggcg aggtagcgat
    45901 ttttttgatt gccagttttg cctgcatgat ttgacgttgc cattttccta gagttgtcac
    45961 attggaaact acttcatcaa tcaggacaaa ttggaaatcg ccaacttctc gccctggtgt
    46021 cagagagtat ctttgtggct gttttggcaa acggccttgt ttcatcaaat catggatgac
    46081 ttggcgaaca tatacgttga tttcctgttg gaccctgaaa cctaagaaca gattcacttg
    46141 gacaataaaa tcagtgtcca tcatatctac ttgatattct ttcgtatatg gctcatccgt
    46201 cacttccaca tgaacaaacc aatagactcg tgctctttta ggccgtttat ctaaaatcga
    46261 atagatgaac tgtcggccaa ctttatcttc agccatatca ggaaccaaga agaccacatt
    46321 tgtctgatag attggtaaag aagtatcatc acgtaattct ctaagctgcg ggatataatc
    46381 tctcaaagac acatcctcgg ttgacgattc gcgaataatg ttcccttgtt cccaaataaa
    46441 catcacgact aagatgatca aaccaatcag aacggctaca aaaccgcctc ggaagaattt
    46501 gacaacactt gagataaaga aaacgacttc tatcgcaccg aagaaaaagg taactaaata
    46561 tgcaagccaa cgagaatgcc cattttggat aagataaaag taaagcagtg ctgttgtcat
    46621 caacatcgtc acagtgatcg atagaccgta agcagcttcc atatgatgag acgttcggaa
    46681 tgtgacaacc actagagaac aggcgatcca taaaatcaga ttgactgctg ggatatacat
    46741 ttgcccgata ctgttcccag gatacatgat ctttaaccga ggcagcagtt tcaacttgat
    46801 tgcttctgaa actagcgtaa acgaacctgt aatcaatgct tgcgaagcaa tgactgcagc
    46861 gactgttgca aaagcaacgc cgatcacgat ccaactttga ggcatcattt ggaagaatgg
    46921 attcaggttc ttgatttgct gtgcttcagg gttttgataa acattcaaca accacgcagc
    46981 ctgcccaaaa taattgataa ccaaacaaat tttgacgtaa ggccagctca gacgaatatt
    47041 tcgtttgccg acatgtccaa gatcggaata aagcgcctct gcacctgtcg tagccaggaa
    47101 aatatttcca agaatgaaca agcctaattt gttatctgca ctaaaaagca gctggatagc
    47161 ataataagga ttcagtgcac ggataactgt ccagtcttga ctgaagttca ttaatcccat
    47221 tacgcctaaa aaagtaaacc aagcgaacat gattggtccg aatgcttttc cgacaatatc
    47281 tgtaccaaag cgttggacca tgaataaaac aaaaataata actaaagtga taatgacgat
    47341 cacgttctgg ttattaccga accggtcaaa aaactgcgga attcctcgca agccttcaat
    47401 cgcagtcgtt accgtcacag caggggtcaa taccccatct gctagtaagg cagctcctcc
    47461 gatcattgca ggaatgatca aatatttccc accttttcgg acaagtgtgt agagtgagaa
    47521 aattccacct tcaccatggt tatctgcatt caatgcaatc acgacatatt tgattgttgt
    47581 cagtagagtc aatgtccaaa aaatcaatga gactgatccg ataacaaaat caggattgat
    47641 tcctgacaat ccgccattat cttcaacaat cgatttcata acgtaaagcg gacttgtccc
    47701 gatgtctccg tagacgactc ccatcgctac aagcaggcca gccattgata tctttttggt
    47761 attgtgcgtt gttgccacgt ttttccctca tttcttgtta tatggatatc ctttaggtaa
    47821 gcttgaatga tagatgatat tcttatccat gacacccgct ttcctaaatt gcttctaatt
    47881 atccaagtaa cacactgaag tgtcaactta tttgaataaa attattcttt ctttggacgt
    47941 tctctgatac gtcttgccac aaatgaataa agagcaaggc aaaaaattgc acccaataat
    48001 gctggtatca aataaaaacc gccgagttcc ggtcccagtg gtcctagtaa aaattcgccg
    48061 agccaactac ctaaaaaacc gataatgata tttccagata cgcctcccgg gacttccttt
    48121 cctaaaattg cgccagctat caaacctaat ataccgccga taaataacga ccataaaaaa
    48181 ttcagcatct cctttccctc ctttatccat atttctcact tttattttac gctttattca
    48241 gaaggactcc aaaaatatgc gccaagaaac tccagaaaaa aagaagttgt aacaaaagtg
    48301 ttcagcttat ttccgtagaa gctgcttctg gaacaccgtg aatcagaggt tgtgaaatca
    48361 gcggtttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    48421 ttttgagcta atatcgaggg acagctgatt gaacatcgtt tattggagag gttgtaaaaa
    48481 agctgttctg catcaagtaa taagaacagc caagtaaaaa cagctcctaa tgtttttaac
    48541 ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaagg
    48601 actgtgacac ggcttttgtc acagtcccct tttttcgaat aattattgtc cccctgtcac
    48661 cttcacgatc acaagtacat tattcctatg aattaaatta tttttcttca tttttcgttg
    48721 aaccatcttc gttcaacatt gcttttcttg acagatttac gcgtccttgt ttatcgattt
    48781 cagttacttt cacaaggatc tcatcaccta gtttaacaac atcttcgact ttattgacac
    48841 gatcgtttgc taattgggaa atatggacaa gtccatcttt tcctttgatc aagttgacga
    48901 atgcaccgaa tttttcgata cggacaactt tccctagata aacttcacca actttgactt
    48961 ctttggtcaa atcttcgatg atcttgatag cttttttgat catttctgca tcagctgaag
    49021 caatacttac ttttccatct tgatcgatat cgattttcac accagtttca tcgatgattc
    49081 cgttgatcgt atcgccgcct ttaccgataa catctttgat tttagcaggt tcgatctgga
    49141 tcatttcgat ttttggtgca tattggctta attcttcacg tggagcagca atcgttgaag
    49201 tcaattcttc taagatttcc atacgtgctt gttttgcttg agttaatgct tcagttagga
    49261 tttgttctgt gattccttga atcttgatat ccatctgtaa agcagtgata ccatcttttg
    49321 taccggcaac tttaaagtcc atatcgccaa gatgatcttc cagcccttgg atatcagtta
    49381 ggatcgtata gttttcacca tcagaaacaa gtcccatcgc aataccggcg actggcgctt
    49441 tgattggtac acctgcatcc atcaatgcta atgtgccagc acagatactt gcttgggaag
    49501 aagaaccatt tgattccaat acttctgcta ctaaacggat cgtataagga aattcttctt
    49561 ctgaagggat tacttgtgct aacgcacgtt cacctaatgc gccatgtccg atttcccgac
    49621 gccctggaga gcctgcacgt cctgtagaac caacagaaaa ttgagggaaa ttgtagtggt
    49681 ggatgaatcg tttgctgact tccaccccta aaccatcaat gatttggtgc tctcctagag
    49741 gtgcgagtgt acaagctgat aaagcttgtg tttgtcctcg tgtgaacaaa cctgatccat
    49801 gtacacgtgg caataagctg acctctgatg acaatggacg gatttcatcc aatttacgcc
    49861 cgtcaggacg gatcttatcg atggtgatca attcacgtac gacatctttt tcaagatctt
    49921 ctgtgatttg tttaacttct ttcaacaagt gatcttcatc ttcatgacct gcaaatttct
    49981 cagcataagc ttcgcggata tcgatcttca cctgctcgat attatcttcg cgagcaagtt
    50041 tttcttcagt cattactgct gctttcattg tagcgtaaga agcatcaaag atttcttttt
    50101 tcaatacttc atcaacttgc agtaaagtca cttccatttt ttctttccct actgcttgaa
    50161 cgatttcttc ttggaaagca actaactctt tgatagcgtc aaaaccaaac agcaatgcac
    50221 caagcatgtc ttcttctgaa acttctttcg cgccgctttc aaccatattg atggcttgtt
    50281 tcgttccggc aacagtcaat tcgatatctg tttgttctgc ttgttctact gttggattca
    50341 agacgtactc gccatttaca cgaccaactt ctacgcctgc gattgggccg tcaaatggga
    50401 tatctgagat tgatagtgct aaagaagaac ctagcattgc tgccatagct ggcgagcagt
    50461 ctgtttctac actcatcacg atgttcgtta cttggacttc attacggaag ccttctgcaa
    50521 acatcgggcg aattggacgg tcgatcaaac gggcagtcaa tgttgcttcc gtacttggac
    50581 ggccttctcg tttgatgaaa cctccaggga ttttccctgc tgcatacatt ttttcttcat
    50641 aattgatcgt taatgggaaa aagtctgtat cttttgcttc tttagaggcc acagctgcac
    50701 ttaagactac tgtatctcca taacggacta aaacggctcc gtttgcttgt tttgctagct
    50761 gaccgacttc gatttgtaag ggacggccgc cccatgtcgt tttgaatact tgtttttctg
    50821 acataacttc tccttttcct tctcgtgaaa gcaagacgct actaaacaaa tgaaaactgc
    50881 cgaaagccgt cttttgagca gctttcattt ggttagtagg agtccatcta gcttgatcac
    50941 ggaattttac ttaattgata tacgagtaaa aaaagtgaga ttcatgtgaa tcccactctt
    51001 tttgactaaa attaacgacg caagcctaaa cgtttgatca attcgcggta acgttgaacg
    51061 tcagttttac gtaagtaagc taacaagtta cggcggtgac caactttttt cattaggccg
    51121 cggtatgaat gatggtcttt tttgtgcacg cgtgcatgtt cattcaagtg gttgatttct
    51181 tcagttaata cagcgatttg tacttctgga gaaccagtat ccccttcatg gcgagcgtat
    51241 tcgttgatga tttcattctt gcgttctttt gaaattgcca tttctttcac ctctttcgat
    51301 tattcccctg cgactgagta aaacgttggt gattcgctta accaagtggc aggtcatgtg
    51361 cctagagcac acagctatac tttacctgaa accgggttga aaatcaagta gttaatcatt
    51421 aagaaaaggt agttttcaaa caatcttact actacttttc catttttgtg gtaaactata
    51481 atgcgaaaat agacttgaaa cacagtcttt taagaaaggg gtacatattc catgattaca
    51541 atggatgata tcattcgtga aggaaatccg accttacgcg aagttgcaaa agaagtttct
    51601 cttccattaa gcgaagaaga tatctcttta ggaaaagaga tgcttgagtt cttaaaaaac
    51661 agccaagatc caatcaaagc tgaagaacta cacttacgag gcggtgttgg cttagctgct
    51721 cctcaattag atatttctaa aagaatcatt gctgttcacg taccaagtcc tgacccagag
    51781 gcagatggac cttctctaag tactgttatg tataatccta aaattttgag ccattctgtt
    51841 caagatgctt gtttagggga aggtgaaggc tgtttatctg ttgatcgaga agtaccagga
    51901 tatgttgttc gccatgccaa aatcactgtg tcttattatg atatgaatgg tgaaaaacac
    51961 aagattcgtt tgaagaatta tgaatcgatc gtcgttcaac atgaaatcga ccatatcaac
    52021 ggtgtgatgt tctatgacca catcaacgat caaaatccat ttgcactaaa agaaggcgta
    52081 ttggtgatcg agtagagaat tggcaggttc cttcgatact atgctacaag aaagctttat
    52141 aaaaaagggg agtgctcaaa agctgcactt ccctttttta tgtttctcca ctaatcgaaa
    52201 tttagataga catctagaaa tcgcaattcc aaaaaattct gtgtattgga tacgcatctt
    52261 ttcttatttt ttcttatttc ttccatatgg gtgtttatct cagagatgat agaatagttt
    52321 ctaggataaa aagtagcagc tacgatgtta tccttcgtag aggattcctg cttcagctag
    52381 tttttgtttg atgtttttca gcatatgctt ttctgggtag cggatcgtat gcagatggat
    52441 gccgcctgtt aattcggata acaaagtagc attacttttt tgataacgtc tcataaagga
    52501 atcgatatct tgtggcgttt tgatgtgcaa agttcctgct aactcaccgt aaaggggatg
    52561 ttccacaatt acatcaatga tttcaccacc attttcaacg attagtctca gttcttcttc
    52621 tgtttgatct tttccatgct ggacagcgat tttactgatg tgcccagtat tggtttcttc
    52681 tgtttcgagg atataacctc ttgctgttgc cacgatactt ttacctgcag ctcgtagtaa
    52741 agcaatatcg cctacaatga tctgacggct gactccaaaa gttttcgcaa acttgctagc
    52801 actcactggt ttttctgtat ttgccaaggt ttgcatgatt tgttttcgcc gtgtctctcc
    52861 ttcgatcaca cttattcctc cttcattttg ttttggtatt cgcgcgagtg tttcatcaaa
    52921 tcaacatacg tgtctgttat tgtttcctta tatcccttat ttttcagcag actaacaact
    52981 ttatccaata cttgctgctc tctttttgca tcataaactg cttggttttc ttcttttttc
    53041 acttgtccta ttttagttac cgtatccatt cttttttcta ataaagcaac caactctcga
    53101 tcgatcatat cgatcttatg acgtaatgtt tcaagagact cttctgttag ttcttcctct
    53161 actgtcttga tgatacgagc aggatttcct gcaatcacta cattgtcggg aaaagatttg
    53221 gtcacaaccg aacctgcgcc gactactacg ttgtccccta gagtgacacc cggcgtgacg
    53281 ataactcctc cgcctaacca aacattatta ccgattttaa tgggttttgc gtactccaga
    53341 ccgctgttac gtttcactgg atgtagtgga tgcgtcgccg tgtaaagctg gacattcgga
    53401 gcgaacatgc agttatcacc gatttcaatc gtgctgacat ctaaaaatgt acaattgaaa
    53461 tttgcataaa aattctcccc aacaaatata ttatatccgt aatcaaaatt gataactggc
    53521 tccatataga tttttttccc tgtacggcca aaagtctctt tcaacaattg ggatcgcagt
    53581 tccgctgatt ctgcttggtt gatgatttgg ctttgacttc ttgcaaacat acggtcggca
    53641 accagctcag gatcattggc aaaataaagc tcgcctgcaa tcattttttc tttttcggtt
    53701 ttgaattcag tcataatctg ctcctaattc ttgtattttt ttgaaaacaa ccgctccgtc
    53761 ttcttgaata aacgtataag gctccgctaa ataaacaggc gtttgattga ttactaagat
    53821 tttggctgac ggctggcgaa actgtaccag atcacaaaac ggatgaactt gaaaactcgt
    53881 tccaacaatg acgatcagct ctgcttggct gacggcataa gccgcctgct ccatgactcc
    53941 atcttgaaac ccttcaccat ataacacgat gtctgggcgt atttgtcctc cgcatgcttg
    54001 atgtttgtcg ctgtgaagat attctttcca gtctaccgtt tgttgacatt ttcgacaatg
    54061 acattgatac agactcccat gaaaattgac gagttgcggg cttttagcct ttgcgtgcaa
    54121 accgtcgatg ttttgggaaa caacccaaac atttttttct ttcgctagat gtgctatttc
    54181 caaatgaatg atattgggtt ttgcttcagg atgataaatc tttttgacaa attgatagaa
    54241 tttatccggt tcattcacca ttgcttgatg actcagcaag tattccggct gctccatccc
    54301 gtggtagatt cctgacaaag atcgatagtc tggcactcca gaaggtgtgg atacccctgc
    54361 accggtcaaa aatgtaattt gctgataagc taatatctct tttgctgctt cgtctactgt
    54421 taattgtttc atgttttgtc acctcacttt ccattatagc ccatactcga acaaatttat
    54481 tgttgaaaag tcagaaaaat gggaataaaa aagtgttatg ggcatttgag caatttatca
    54541 caatgttcac tgacaaaaga atgaaaacgt gttacaatac tgctgtataa aagtgaggtg
    54601 aatcgatatg ttaccattct ttatttattg gggcgttatt ctggcttgta tcgcctggtt
    54661 agcgttaagc gtttatttct ctgtttttta tcttgtacgc aaagaaaatg gaaatctttg
    54721 ggcttttgct ttattcaacg tgattgccgc aattgtttta gccattacat tagccgtata
    54781 tcgaacatgg ggctggggaa tcacccaata ctcaagtttg atttatctaa tattagccat
    54841 ttatggcgtc gttgttcttt tgcaagcgat cctaggacgt gagccaaaga aagcggcagc
    54901 ttaaatgatc gagatggaac tggacacagg aaattttctg ttctagttct ttttttattg
    54961 tattacatac atttttgtgt ataattatac tatcgtttgc aatggataga tgattcacat
    55021 tttgattgtt ttttcggtaa aagtaaagat ttctatattt atcagtattt tttcaaaaac
    55081 cagcagttat agattgacaa agcctctcga gtatcgtaaa ctttttttgt ataaataact
    55141 gaatacgaga tgcctcaatc aatgaggtag aggtcgcgag aatcattaac cctgtggagt
    55201 gaagcaaaca tggagaagca ggaataggga ggatcgccga aatgtgcact tttttgcttg
    55261 gagtgtatgt tgggacgcaa gttaagagct tgcggactgt cttagcagtg atgctaagtt
    55321 gcgctatgtt ttgtgagagg tttatcacct tatggaattt tagcggcctt tttatatcta
    55381 taaatcggtc gctttttatt ttttcagata aagctttacc gcatttcatc tagaccgctc
    55441 gtgtatcata taatcagaaa gggagaaaaa aatgaaaaaa aatatggttt cagttgccgt
    55501 actatttgct tttgtcttct cactattcag tgtgggatct ctggcaaatg cagatgaaaa
    55561 aacaccgagc gatgagtttc gcgtaggtat ggaggctggg tatgcaccct tcaactggtc
    55621 tcaacaaaca gatgagaata atgccttgcc aattcaaggc caaaatagtt acgccggcgg
    55681 ttatgatgtt caaatcgcta aaaaagtagc agatggttta gggaaaaaat tagtcatcgt
    55741 ccaaacgaaa tgggatggtc tggctcctgc ccttcaatca ggcaagatcg acgcgatcat
    55801 cgccggaatg agccctactg ctgaacgacg aaaagaaatt gattttaccg atccttatta
    55861 cgaatcacaa ttagtagtag tagttcaaaa agatagcaaa tatgcaaaag caaaaagttt
    55921 agcagatctt tctggagcaa agatcactgc tcagttaaat acattccact atactgtgat
    55981 cgatcagata ccagatgtgc agaaacaaca agcaatggat aatttctcag caatgagaac
    56041 agctttagct tctggaatga tcgatggtta tgtcagcgaa cgtccggaag gtgtaacggc
    56101 aactagtgtg aacaaagact tggaaatgct tgaattcagc ggtgcagatg gcttccagac
    56161 aaacccggaa gatgttcaga tttctgttgg tatgcgtaaa ggcgacccgg atatccaaaa
    56221 agtaaaccag attctttctg gcatctctaa cgatgaacga accaaaatta tggaccaagc
    56281 agtcaaagat cagcctgctg caactgatag tgaagaaaaa cctggcatca tttctgattt
    56341 cactacgatt tggaaacaat acggcaatat gttcttacga ggtgctggac ttaccttgtt
    56401 catcgcattg atcgggacag ttgtcggtac tacacttgga ttactgattg gtgtcttccg
    56461 ttcggtacct gaatcaggaa atgcactgac tcgcttcttc caaaaaatcg gaaatgcact
    56521 tctttctatc tatgtcgaag tattccgcgg aacaccgatg atggtacaag caatggtcat
    56581 cttctacggt ctggcactcg cttttggcat ttcattgaac cgaacgattg ccgctttgtt
    56641 tattgtctct ctcaatactg gagcttacat gacagagatc gttcgtgggg ggatttttgc
    56701 agtagatgaa gggcagttcg aagctgcgca agccattggt atgacccatg gccaaacgat
    56761 gagaaaagtt gttattccac aagttttacg gaatatcttg ccagcaacag gaaatgagtt
    56821 tgttatcaat atcaaagata cagcagtttt aagtgtaatc ggcgtagctg atttgttctt
    56881 ccaaggaaac tctgcttccg gtgcgaattt ccaattcttc caaacattta cgatcgtcgg
    56941 gatcatctac ttagtcatga cctttacgat caccagaatc ctgcgggcgg tagaaaagaa
    57001 aatcgatggt ccttcagctt ataccaagct tgaagaaatc gacaattcaa atttgcagga
    57061 ataaggagga agaatcatga atgaaaaaat tattgaaatc aaacatctaa gaaaaagctt
    57121 cggtgaaaat gaagtattga aagatatcag cgtaacagtc aataaaggtg aagttgtgac
    57181 gatcatcggt tcttccggtt ctggtaaatc tactttcttg cgctgtatca atttactgga
    57241 aaaaccaaca ggcggcgaga tcatttataa tggagaaaat gtattagctc ctaaatataa
    57301 tttacctaaa tatcgaacga atctaggtat ggttttccaa tccttcaacc tgttcaacaa
    57361 tatgaatgtc ttggaaaact gtatgtctgg tcaaacaact gtcctgaaac gagataaaga
    57421 aactgccaaa aaagtagcaa tcgaaaatct ggaaaaagtt ggtatggatc gttatatcga
    57481 agccaaacca gcacaattat ctggtggaca gaaacagcgt gtcgcaattg ctcgggcatt
    57541 atcaatgaat ccagatgtat tgctattcga cgaacctact tcggcgttag accctgaaat
    57601 ggttggcgaa gtgcttaata cgatcaaaga cttagcacat actggcttga caatgcttat
    57661 cgtgacgcat gagatggaat ttgcaaaaga tgtatctgac cgtgtcattt ttatggataa
    57721 aggtgtgatt gcagaagaag gtacaccaga agatatcttt gtccatccga aagaagaacg
    57781 tacaaaagaa ttcctttcac gtattttaaa tgcttaggat caatagctga ttgggacaaa
    57841 gagctgcggt gtttaccgca gttctttttt atgaattact cataaaaggt aaatttctga
    57901 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
    57961 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
    58021 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
    58081 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
    58141 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
    58201 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
    58261 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
    58321 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
    58381 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
    58441 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
    58501 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
    58561 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
    58621 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
    58681 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
    58741 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
    58801 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
    58861 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
    58921 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
    58981 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
    59041 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
    59101 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
    59161 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
    59221 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
    59281 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
    59341 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
    59401 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
    59461 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
    59521 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
    59581 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
    59641 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
    59701 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
    59761 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
    59821 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
    59881 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
    59941 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca ataactctca
    60001 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
    60061 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
    60121 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
    60181 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
    60241 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
    60301 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
    60361 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
    60421 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
    60481 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
    60541 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
    60601 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
    60661 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
    60721 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
    60781 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
    60841 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
    60901 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
    60961 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
    61021 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
    61081 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
    61141 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
    61201 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
    61261 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
    61321 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
    61381 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
    61441 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
    61501 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
    61561 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
    61621 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
    61681 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
    61741 acatttttgc ata
//
LOCUS       DACCPY010000004        61633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCPY010000004 DACCPY010000000
VERSION     DACCPY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.004"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(16..702)
                     /gene="gpmA"
                     /locus_tag="IVO69_000196"
     CDS             complement(16..702)
                     /gene="gpmA"
                     /locus_tag="IVO69_000196"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7399006.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(874..1455)
                     /locus_tag="IVO69_000197"
     CDS             complement(874..1455)
                     /locus_tag="IVO69_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7399007.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="IVO69_000198"
     CDS             complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="IVO69_000198"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7399008.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2590..3003
                     /gene="rpsL"
                     /locus_tag="IVO69_000199"
     CDS             2590..3003
                     /gene="rpsL"
                     /locus_tag="IVO69_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7399009.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3087..3557
                     /gene="rpsG"
                     /locus_tag="IVO69_000200"
     CDS             3087..3557
                     /gene="rpsG"
                     /locus_tag="IVO69_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7399010.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3651..5738
                     /gene="fusA"
                     /locus_tag="IVO69_000201"
     CDS             3651..5738
                     /gene="fusA"
                     /locus_tag="IVO69_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7399011.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5910..7097
                     /gene="tuf"
                     /locus_tag="IVO69_000202"
     CDS             5910..7097
                     /gene="tuf"
                     /locus_tag="IVO69_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7399012.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7504..7812
                     /gene="rpsJ"
                     /locus_tag="IVO69_000203"
     CDS             7504..7812
                     /gene="rpsJ"
                     /locus_tag="IVO69_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7399013.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7843..8472
                     /gene="rplC"
                     /locus_tag="IVO69_000204"
     CDS             7843..8472
                     /gene="rplC"
                     /locus_tag="IVO69_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7399014.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8500..9123
                     /gene="rplD"
                     /locus_tag="IVO69_000205"
     CDS             8500..9123
                     /gene="rplD"
                     /locus_tag="IVO69_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7399015.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9123..9413
                     /gene="rplW"
                     /locus_tag="IVO69_000206"
     CDS             9123..9413
                     /gene="rplW"
                     /locus_tag="IVO69_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7399016.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9452..10285
                     /gene="rplB"
                     /locus_tag="IVO69_000207"
     CDS             9452..10285
                     /gene="rplB"
                     /locus_tag="IVO69_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7399017.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10328..10606
                     /gene="rpsS"
                     /locus_tag="IVO69_000208"
     CDS             10328..10606
                     /gene="rpsS"
                     /locus_tag="IVO69_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7399018.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10629..10976
                     /gene="rplV"
                     /locus_tag="IVO69_000209"
     CDS             10629..10976
                     /gene="rplV"
                     /locus_tag="IVO69_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7399019.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10990..11646
                     /gene="rpsC"
                     /locus_tag="IVO69_000210"
     CDS             10990..11646
                     /gene="rpsC"
                     /locus_tag="IVO69_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7399020.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11649..12083
                     /gene="rplP"
                     /locus_tag="IVO69_000211"
     CDS             11649..12083
                     /gene="rplP"
                     /locus_tag="IVO69_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7399021.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12073..12261
                     /gene="rpmC"
                     /locus_tag="IVO69_000212"
     CDS             12073..12261
                     /gene="rpmC"
                     /locus_tag="IVO69_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7399022.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12286..12552
                     /gene="rpsQ"
                     /locus_tag="IVO69_000213"
     CDS             12286..12552
                     /gene="rpsQ"
                     /locus_tag="IVO69_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7399023.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12610..12978
                     /gene="rplN"
                     /locus_tag="IVO69_000214"
     CDS             12610..12978
                     /gene="rplN"
                     /locus_tag="IVO69_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7399024.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13015..13323
                     /gene="rplX"
                     /locus_tag="IVO69_000215"
     CDS             13015..13323
                     /gene="rplX"
                     /locus_tag="IVO69_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7399025.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13351..13890
                     /gene="rplE"
                     /locus_tag="IVO69_000216"
     CDS             13351..13890
                     /gene="rplE"
                     /locus_tag="IVO69_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7399026.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13909..14094
                     /locus_tag="IVO69_000217"
     CDS             13909..14094
                     /locus_tag="IVO69_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7399027.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14130..14528
                     /gene="rpsH"
                     /locus_tag="IVO69_000218"
     CDS             14130..14528
                     /gene="rpsH"
                     /locus_tag="IVO69_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7399028.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14560..15096
                     /gene="rplF"
                     /locus_tag="IVO69_000219"
     CDS             14560..15096
                     /gene="rplF"
                     /locus_tag="IVO69_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7399029.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15257..15613
                     /gene="rplR"
                     /locus_tag="IVO69_000220"
     CDS             15257..15613
                     /gene="rplR"
                     /locus_tag="IVO69_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7399030.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15634..16134
                     /gene="rpsE"
                     /locus_tag="IVO69_000221"
     CDS             15634..16134
                     /gene="rpsE"
                     /locus_tag="IVO69_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7399031.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16149..16328
                     /gene="rpmD"
                     /locus_tag="IVO69_000222"
     CDS             16149..16328
                     /gene="rpmD"
                     /locus_tag="IVO69_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7399032.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16372..16812
                     /gene="rplO"
                     /locus_tag="IVO69_000223"
     CDS             16372..16812
                     /gene="rplO"
                     /locus_tag="IVO69_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7399033.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16812..18107
                     /gene="secY"
                     /locus_tag="IVO69_000224"
     CDS             16812..18107
                     /gene="secY"
                     /locus_tag="IVO69_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7399034.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18169..18816
                     /locus_tag="IVO69_000225"
     CDS             18169..18816
                     /locus_tag="IVO69_000225"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7399035.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19007..19225
                     /gene="infA"
                     /locus_tag="IVO69_000226"
     CDS             19007..19225
                     /gene="infA"
                     /locus_tag="IVO69_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7399036.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19258..19374
                     /gene="rpmJ"
                     /locus_tag="IVO69_000227"
     CDS             19258..19374
                     /gene="rpmJ"
                     /locus_tag="IVO69_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7399037.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19392..19757
                     /gene="rpsM"
                     /locus_tag="IVO69_000228"
     CDS             19392..19757
                     /gene="rpsM"
                     /locus_tag="IVO69_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7399038.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19785..20174
                     /gene="rpsK"
                     /locus_tag="IVO69_000229"
     CDS             19785..20174
                     /gene="rpsK"
                     /locus_tag="IVO69_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7399039.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20254..21192
                     /locus_tag="IVO69_000230"
     CDS             20254..21192
                     /locus_tag="IVO69_000230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7399040.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21220..21600
                     /gene="rplQ"
                     /locus_tag="IVO69_000231"
     CDS             21220..21600
                     /gene="rplQ"
                     /locus_tag="IVO69_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7399041.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21872..22711
                     /locus_tag="IVO69_000232"
     CDS             21872..22711
                     /locus_tag="IVO69_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7399042.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22687..23556
                     /locus_tag="IVO69_000233"
     CDS             22687..23556
                     /locus_tag="IVO69_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7399043.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23546..24343
                     /locus_tag="IVO69_000234"
     CDS             23546..24343
                     /locus_tag="IVO69_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7399044.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24546..25292
                     /gene="truA"
                     /locus_tag="IVO69_000235"
     CDS             24546..25292
                     /gene="truA"
                     /locus_tag="IVO69_000235"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7399045.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25625..28294)
                     /locus_tag="IVO69_000236"
     CDS             complement(25625..28294)
                     /locus_tag="IVO69_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7399046.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28532..28720)
                     /locus_tag="IVO69_000237"
     CDS             complement(28532..28720)
                     /locus_tag="IVO69_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7399047.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28990..29352
                     /locus_tag="IVO69_000238"
     CDS             28990..29352
                     /locus_tag="IVO69_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7399048.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29404..31086
                     /locus_tag="IVO69_000239"
     CDS             29404..31086
                     /locus_tag="IVO69_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7399049.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31203..33239
                     /gene="recG"
                     /locus_tag="IVO69_000240"
     CDS             31203..33239
                     /gene="recG"
                     /locus_tag="IVO69_000240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7399050.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33288..34289
                     /gene="plsX"
                     /locus_tag="IVO69_000241"
     CDS             33288..34289
                     /gene="plsX"
                     /locus_tag="IVO69_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7399051.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34411..34653
                     /gene="acpP"
                     /locus_tag="IVO69_000242"
     CDS             34411..34653
                     /gene="acpP"
                     /locus_tag="IVO69_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7399052.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34921..35925
                     /locus_tag="IVO69_000243"
     CDS             34921..35925
                     /locus_tag="IVO69_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399053.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35926..36870
                     /locus_tag="IVO69_000244"
     CDS             35926..36870
                     /locus_tag="IVO69_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399054.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36870..37832
                     /locus_tag="IVO69_000245"
     CDS             36870..37832
                     /locus_tag="IVO69_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399055.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37859..38773
                     /locus_tag="IVO69_000246"
     CDS             37859..38773
                     /locus_tag="IVO69_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399056.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38799..40580
                     /locus_tag="IVO69_000247"
     CDS             38799..40580
                     /locus_tag="IVO69_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7399057.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40928..41614
                     /locus_tag="IVO69_000248"
     CDS             40928..41614
                     /locus_tag="IVO69_000248"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7399058.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41634..45215
                     /gene="smc"
                     /locus_tag="IVO69_000249"
     CDS             41634..45215
                     /gene="smc"
                     /locus_tag="IVO69_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7399059.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45224..46033
                     /locus_tag="IVO69_000250"
     CDS             45224..46033
                     /locus_tag="IVO69_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7399060.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46044..47042
                     /gene="ftsY"
                     /locus_tag="IVO69_000251"
     CDS             46044..47042
                     /gene="ftsY"
                     /locus_tag="IVO69_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7399061.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47229..49496
                     /gene="gshAB"
                     /locus_tag="IVO69_000252"
     CDS             47229..49496
                     /gene="gshAB"
                     /locus_tag="IVO69_000252"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7399062.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49585..50037)
                     /locus_tag="IVO69_000253"
     CDS             complement(49585..50037)
                     /locus_tag="IVO69_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7399063.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50187..51137
                     /locus_tag="IVO69_000254"
     CDS             50187..51137
                     /locus_tag="IVO69_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7399064.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51130..52089
                     /locus_tag="IVO69_000255"
     CDS             51130..52089
                     /locus_tag="IVO69_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7399065.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52086..52841
                     /locus_tag="IVO69_000256"
     CDS             52086..52841
                     /locus_tag="IVO69_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399066.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52864..53817
                     /locus_tag="IVO69_000257"
     CDS             52864..53817
                     /locus_tag="IVO69_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7399067.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54340..54636)
                     /locus_tag="IVO69_000258"
     CDS             complement(54340..54636)
                     /locus_tag="IVO69_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7399068.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            54991..55347
                     /locus_tag="IVO69_000259"
     CDS             54991..55347
                     /locus_tag="IVO69_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7399069.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55357..56256
                     /locus_tag="IVO69_000260"
     CDS             55357..56256
                     /locus_tag="IVO69_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7399070.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56773..57504
                     /locus_tag="IVO69_000261"
     CDS             56773..57504
                     /locus_tag="IVO69_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7399071.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57649..59676
                     /locus_tag="IVO69_000262"
     CDS             57649..59676
                     /locus_tag="IVO69_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7399072.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59784..60881
                     /locus_tag="IVO69_000263"
     CDS             59784..60881
                     /locus_tag="IVO69_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7399073.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            61188..>61633
                     /locus_tag="IVO69_000264"
     CDS             61188..>61633
                     /locus_tag="IVO69_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7399074.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        20367 a        11022 c        13143 g        17101 t
ORIGIN      
        1 tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat catttaattc
       61 ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt cagagatacc
      121 ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta aaacagtttt
      181 gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc gttctaatgt
      241 cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt aacgacggtc
      301 atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat cgtaagaacg
      361 acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt tgttcaaacc
      421 ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag
      481 ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta aaacagaagt
      541 gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt cttttgcttc
      601 ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt tcaatgcatt
      661 ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct ctccttttaa
      721 gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt ttggaagaga
      781 tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata taaaaaaacg
      841 aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt
      901 ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat
      961 aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc
     1021 atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa
     1081 tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata
     1141 gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg
     1201 atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag
     1261 ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac
     1321 agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa
     1381 gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata
     1441 accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta
     1501 aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt
     1561 tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac
     1621 gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac
     1681 taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag
     1741 atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt
     1801 acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata
     1861 agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc
     1921 aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc
     1981 gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa
     2041 atcgactacg gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc
     2101 ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg
     2161 accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc
     2221 gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc
     2281 cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa
     2341 aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg
     2401 acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag
     2461 aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt
     2521 ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg
     2581 aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa
     2641 aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac
     2701 gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa
     2761 ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact
     2821 gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt
     2881 ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact
     2941 gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct
     3001 taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa
     3061 caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg
     3121 ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg
     3181 ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag
     3241 aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg
     3301 ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc
     3361 gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg
     3421 gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag
     3481 gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg
     3541 ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata
     3601 gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag
     3661 aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa
     3721 cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc
     3781 atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca
     3841 cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag
     3901 gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa
     3961 ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa
     4021 caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact
     4081 tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat
     4141 taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg
     4201 aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc
     4261 gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag
     4321 aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta
     4381 tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga
     4441 acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata
     4501 tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg
     4561 acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt
     4621 taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa
     4681 caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag
     4741 aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta
     4801 ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag
     4861 aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg
     4921 tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa
     4981 caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta
     5041 tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa
     5101 ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc
     5161 aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg
     5221 aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt
     5281 tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta
     5341 aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg
     5401 cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca
     5461 tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg
     5521 ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg
     5581 cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag
     5641 gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag
     5701 aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct
     5761 gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg
     5821 atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat
     5881 atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac
     5941 cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg
     6001 caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg
     6061 atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg
     6121 aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa
     6181 acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg
     6241 gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca
     6301 tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg
     6361 aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag
     6421 ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg
     6481 aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat
     6541 tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc
     6601 gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag
     6661 aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg
     6721 aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg
     6781 gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat
     6841 acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac
     6901 aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa
     6961 tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg
     7021 aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta
     7081 cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa
     7141 tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat tgcaggatca
     7201 ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt cagtattttc
     7261 taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt
     7321 tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga
     7381 atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg
     7441 agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac
     7501 aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat
     7561 caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca
     7621 attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa
     7681 gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca
     7741 acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa
     7801 atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta
     7861 gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact
     7921 gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac
     7981 gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa
     8041 ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt
     8101 gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac
     8161 attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac
     8221 ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg
     8281 ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc
     8341 aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc
     8401 ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa atcagctgtg
     8461 aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc
     8521 aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa
     8581 cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga
     8641 acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa
     8701 aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc
     8761 gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt
     8821 ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa
     8881 ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct
     8941 atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca
     9001 cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta
     9061 tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca
     9121 taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca
     9181 tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac
     9241 aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg
     9301 cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg
     9361 ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa
     9421 tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc
     9481 aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga
     9541 aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac
     9601 tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa
     9661 caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa
     9721 catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact
     9781 agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct
     9841 tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa
     9901 aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa
     9961 atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc
    10021 tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg
    10081 ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca
    10141 cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg
    10201 gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat
    10261 tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag
    10321 gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa
    10381 agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc
    10441 aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt
    10501 accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag
    10561 aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat
    10621 aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt
    10681 cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa
    10741 tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt
    10801 cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg
    10861 aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag
    10921 cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag
    10981 ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac
    11041 tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga
    11101 atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa
    11161 cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt
    11221 aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga
    11281 atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa
    11341 ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc
    11401 caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac
    11461 ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta
    11521 cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt
    11581 aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg
    11641 aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc
    11701 gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg
    11761 attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca
    11821 tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag
    11881 caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa
    11941 aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt
    12001 tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg
    12061 gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat
    12121 caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa
    12181 ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg
    12241 ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga
    12301 aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc
    12361 gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag
    12421 aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg
    12481 gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca
    12541 gttattatct aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg
    12601 atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt
    12661 gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat
    12721 gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc
    12781 gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt
    12841 aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt
    12901 atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta
    12961 gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt
    13021 gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt
    13081 gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa
    13141 aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca
    13201 attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac
    13261 aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa
    13321 taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa
    13381 gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa
    13441 gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta
    13501 gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct
    13561 aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca
    13621 ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt
    13681 gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta
    13741 ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt
    13801 ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg
    13861 acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa
    13921 tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc
    13981 gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc
    14041 cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta
    14101 aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc
    14161 taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa
    14221 aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat
    14281 acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg
    14341 aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag
    14401 ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag
    14461 gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg
    14521 tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa
    14581 gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa
    14641 ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga
    14701 aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca
    14761 actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta
    14821 gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt
    14881 ggttactcac atccagtaga aatcacacca ccagctggag taactgttga agtaccagct
    14941 aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac
    15001 attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa
    15061 tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag
    15121 ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt
    15181 gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa
    15241 taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca
    15301 tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg
    15361 ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag
    15421 tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt
    15481 cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg
    15541 tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg
    15601 actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg
    15661 gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt
    15721 cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt agggtttggt
    15781 acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa
    15841 aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt
    15901 agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga
    15961 ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc
    16021 tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt
    16081 gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga
    16141 caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa
    16201 accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc
    16261 cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag
    16321 aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt
    16381 catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact
    16441 tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc
    16501 ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa
    16561 cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac
    16621 cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa
    16681 aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg
    16741 aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc
    16801 gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga
    16861 aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac
    16921 caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga
    16981 acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat
    17041 acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg
    17101 aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga
    17161 cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc
    17221 aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa
    17281 cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa
    17341 atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag
    17401 aaatttatga agattacttt ataaatattg atccttcaga catatggaaa tctgctattt
    17461 ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac
    17521 aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca
    17581 gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt
    17641 ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact
    17701 ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct acgatttata
    17761 cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat
    17821 tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga
    17881 ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg
    17941 gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg
    18001 gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt
    18061 tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa agcgtgttga
    18121 ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt
    18181 ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat
    18241 ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct
    18301 cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca
    18361 aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat
    18421 ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat
    18481 aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta
    18541 gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca
    18601 aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat
    18661 aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg
    18721 gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct
    18781 gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc
    18841 attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc
    18901 tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc
    18961 attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga
    19021 tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga
    19081 actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat
    19141 ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg
    19201 ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg
    19261 aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga
    19321 cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag
    19381 gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag
    19441 tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg
    19501 ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc
    19561 gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca
    19621 tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg
    19681 ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg
    19741 ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag
    19801 tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta
    19861 cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat
    19921 cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg
    19981 cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag
    20041 ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag
    20101 aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac
    20161 gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag
    20221 acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc
    20281 gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg
    20341 tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct
    20401 gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt
    20461 agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc
    20521 caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat
    20581 attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct
    20641 gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca
    20701 gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca
    20761 cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt
    20821 gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt
    20881 ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca
    20941 aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg
    21001 acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc
    21061 aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta
    21121 ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt
    21181 aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga
    21241 cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac
    21301 gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg
    21361 atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt
    21421 aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa
    21481 aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt
    21541 ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa
    21601 aatcacacta ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga
    21661 tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca
    21721 ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg
    21781 cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta
    21841 gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat
    21901 aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat
    21961 tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc
    22021 caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat
    22081 attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatgattt ttcaaaatcc
    22141 agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca
    22201 agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat
    22261 ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc
    22321 cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat
    22381 gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag
    22441 tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat
    22501 actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc
    22561 tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc
    22621 actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga
    22681 ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt
    22741 gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta
    22801 gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag
    22861 ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac ggataataag
    22921 aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta
    22981 ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa
    23041 gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat
    23101 ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt
    23161 gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc
    23221 cagggcagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact
    23281 attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg
    23341 ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa
    23401 tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg
    23461 gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc
    23521 gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc
    23581 tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt
    23641 tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac
    23701 attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc
    23761 attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga
    23821 aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt
    23881 tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc
    23941 gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt
    24001 cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat
    24061 ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg
    24121 aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag
    24181 cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga
    24241 aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc
    24301 ttttgtatta ttaactgtgc tactgatctt tttaagagga taaacgttca gtgggccgct
    24361 atctattctt ttcttgctga tctgtttgca agaaaggatg caagcagtgt atgacaagaa
    24421 atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga
    24481 acgaaaaaat tttatagaaa aatcattatt ccagataacc ttttaggaac aaaggagaga
    24541 aaaacatggt acgttacaaa gcaatcattg cgtacgacgg aacaaatttt aatggattcc
    24601 aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta caaaaaatgg
    24661 caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga gtacatgcga
    24721 tcgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga atgcgtttcg
    24781 cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt gtttcagaag
    24841 acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg gatataggga
    24901 aaccacgcag tccttttcga cgccattatg caagttattt tccttaccca cttgatttgg
    24961 aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc tctttttgtg
    25021 catcaggaag ctcgatagag gataaaatac gtacgatcta cgaggctaag atggaggtaa
    25081 atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg tataagatga
    25141 tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct gaagatagta
    25201 tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca caccctgaag
    25261 gactttattt gtatgaagtg aaatatgaat aagaggttat gatagaagtg gtcagcttca
    25321 agaaataaga aggtatttac gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt
    25381 ttcgaagaag ctacttctgg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    25441 tctgagtcac aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    25501 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    25561 aaacgatgga acgttgtttt gattgccctt ggtgcggaca gtctgaacag gttccatcgt
    25621 ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct attgctggat
    25681 caatcgtgcc ataaaagggg cgattgcggt caaggtacca gacataaaga ataaacaatg
    25741 caatacatag gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag taactgaaag
    25801 tgacagtatg tttacctgct ggaactttta tcatcaagaa tgcgtctttg aaattgctga
    25861 ccttgactgg tttcccatca attttagctt tccatccttt gtcataagga atagttgtca
    25921 ccaacatgga atcttcagcg gtatttacag tcccagaagc ttttctgcca gtggcttcca
    25981 tttcgacacc tttttctttg atctgattca ttgcggcttg ataggctaca acatctagac
    26041 caacgacttt aggctccatg aaacttattt ctttcgtccc ataaaaactt gcagtaaatg
    26101 tcactgttgt ctcttgagga taatagccaa gatcatagta ttgaccagaa atgttgattt
    26161 gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact agaactttct aattgcgcaa
    26221 aattggttgg gaacaaactt aagtaagctt gtgtatttgc aggcacatgg acttcccaag
    26281 tgagctcttt tggctggtta tctgctgttt ctgagtaagt gacgccagtc gttgtctttg
    26341 tgatcgtcgt attcaactga ttcttcaaag taggtgtata gaatgtaaaa tactgttcat
    26401 ttagaccaga caatgtattg atcaagtttg tctgactggt caaattatct gtcactggct
    26461 gctcaacatc attaacggac aacggtgcag taaatgctag tggcaatgca ttgacattct
    26521 gatacagtgt atagtcgccg ctttgaccag cttggcggaa gccatattta ctgaacgtgc
    26581 tttcgttttt agaaatgtta tatttgattc ctgtaaaccc atccatcagc aaagtattat
    26641 tgttataacg gatattcaaa tttgtccctc ttgaacggaa acctaattta tccaggtaag
    26701 aggaagaatt acgatttctt atagaagaaa acaaactgat accactgtaa ccatagttga
    26761 tcgaatcgtt tgcagatact ggatccagat tttccaagcg gtaaaacgag tcggaatctt
    26821 tttttgtttc atttacaaga gctttcaagt caggataagg atcagtatat aaactgcggg
    26881 aagcataatt ccaatcatca agaatgccat ggatcatact gttcgtattg accagtgctt
    26941 cagcagacat gatcaataat aacaatatac tcagatgatt gaatgaaagt tttttatgct
    27001 gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat acaaaggaag
    27061 tcatcgtcac ataggtgtag cttccttcag gtttgatgcc ccatgccacc gcaaagagag
    27121 cagctacgac caaaatgatt cctaacaaca gcccaccatc gtcttgttct atcttttccc
    27181 atccttttcc tgcaagcagg agcactaaga aagagaacag ataggcataa cggaataaaa
    27241 acatattcgg tgcatgcata ccatgccaga acaaattcaa tggaacaaga taaaaacttg
    27301 cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt actttccgac
    27361 tggcaaaata aaacagacag aggactaatg gtagcagacc tgcgtaaacg aacggaatcg
    27421 atccgtattt tgtcgtgtca taaacaccaa tcatattttt catgattaga tccagataag
    27481 aagtcgcttc tgttttcaac gtagtgatct gactcagtgc ctcgccattt gaccgaagat
    27541 ccagcactgc tggcaataca atgatcattg aagcgccgcc tgccagtaaa gaagtgatac
    27601 cataaggaac aatcgttttt ttatattctg accagtttgt caatagacgg gcgataaaat
    27661 ataagaaaga aaatacgcca atcataaaac ccatataaaa actggataaa aacaagagta
    27721 aataactgat aaataaaagt ttcggccgtt taagatccat aacacgatga atccccaaaa
    27781 tcactaatgg aagataaaca aatgcatcca gccacatgat gatctccgaa tgtgcagtga
    27841 taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga ggaatcttga
    27901 acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc gaccttaagc aaagtcagaa
    27961 catacagtgc atctggcatc atctggtttg gaaaaaagaa caccagtggc gtgaagatcc
    28021 cgcctagata gtaagaaatt aaagataaat aattcaatcc tagagaagca ttccatgtat
    28081 aaaaaatact ttgctttcca agaagaacat ttcggaagct ggcatgaaaa ttagaaaact
    28141 gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc aggatagatt ccgatactta
    28201 gataaacaag tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt
    28261 tttctctaaa gaatgacttt aaattttttt tcatatttat tttccaccaa atttctatga
    28321 agttgtattc agtttatcat atccttgttt ttctctgctt aaaaagagtc tagttttcac
    28381 aaaaaaataa aatttagaat aaataacttc ataatttgtt cgtataacaa aaataaacgc
    28441 aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt
    28501 tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt cctgatttca
    28561 aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg cgaacttttt
    28621 gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg ttgtttccac
    28681 tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc tcctttgcct
    28741 tgatttggag caatagaata attcagctca taccagacta acatatcata tagaatggga
    28801 atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt gtaaattaaa
    28861 caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg ccttttattt
    28921 caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac ccgttaagga
    28981 ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat taccaatgat
    29041 gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc
    29101 agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa ttactcaaga
    29161 ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac gattatcagc
    29221 tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa atacaatcta
    29281 gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg tgttcgtgta
    29341 ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag gaggattatc
    29401 taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg
    29461 aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc tgtgccagat
    29521 ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc agtagtagat
    29581 tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt gctgatgggt
    29641 gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc taagcaaatt
    29701 ccagatgttg tctcattaaa tgcttctgat cttgctgcag ccttcactca tggagttgaa
    29761 actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt agctagagaa
    29821 gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga agtaatgaca
    29881 gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc cagacttgct tccagtccta
    29941 aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta tgagggcttt
    30001 ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc tccagcagaa
    30061 atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa
    30121 gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg acctacagtc
    30181 gatagtacat tcgattacga agaattccgt aactatttag atggtatagg agattcttta
    30241 cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa
    30301 gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga caacatgcgc
    30361 ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa
    30421 ataccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg
    30481 tttgaaagtt taggcgctaa ttatgttatc agtggtggac aaacaatgaa tccaagtacg
    30541 gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac
    30601 aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca
    30661 gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa
    30721 gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt
    30781 caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac
    30841 tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg
    30901 ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt
    30961 gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct
    31021 gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct
    31081 gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg
    31141 ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat
    31201 ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa aaaagagcag
    31261 ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat tatccttttc
    31321 gctacgaaga tattcaggaa agaaatctaa acgagatcca ggatcaagaa aaggttactt
    31381 tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa aaaagccgat
    31441 tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt aatcaacctt
    31501 atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa
    31561 agcgtaaagc cttgaacggg atgaagattt taggttctca aacaatggat gacttttcgc
    31621 cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta atcaaaaaag
    31681 ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt gtggagaaat
    31741 atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag
    31801 aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc
    31861 ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc ctttatgatg
    31921 tccaaaaatt gaaacaattt actcaaaagc taccttttga actgacaaat gcacaaaaac
    31981 gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag cgacttttgc
    32041 agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta
    32101 cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa caacatatgg
    32161 aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgatt acggggtcaa
    32221 cgaaagcaaa agaacgacgt gagctccttg aaaaactaga gcaaggggaa attaacgtcg
    32281 tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta ggtctagtta
    32341 ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt gaaaaaggct
    32401 ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta gctattacag
    32461 catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt atcccgatcg
    32521 aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg aaaaaagaac
    32581 tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca gaagcattag
    32641 atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg
    32701 aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct
    32761 tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg
    32821 tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc
    32881 accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta
    32941 atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt
    33001 ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc
    33061 aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg
    33121 tagccagaga agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt
    33181 atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc
    33241 agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg
    33301 tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc
    33361 agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat
    33421 acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg
    33481 atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag
    33541 cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag
    33601 cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta
    33661 ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg
    33721 cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta
    33781 aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa
    33841 aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt
    33901 ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag
    33961 atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga
    34021 atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt
    34081 taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg
    34141 ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg
    34201 ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag
    34261 tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt
    34321 gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg
    34381 acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta
    34441 gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac
    34501 atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa
    34561 gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc
    34621 gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa
    34681 aagctgcatg cagcttttta tccttctttt tttaatcttt ttgacacgta aaagtcattt
    34741 ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcggt tttctatata
    34801 attattaata attcattaca caaagataag aataaccttt gttataaaac gatttattct
    34861 gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga
    34921 ttgtccgata accaattatt agaagttcag aacctacata caggattccg actcaaagat
    34981 gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca
    35041 atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt
    35101 gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac
    35161 ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat
    35221 ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc
    35281 tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa
    35341 gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt
    35401 atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca
    35461 gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa
    35521 gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta
    35581 gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt
    35641 gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata
    35701 ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg
    35761 aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc
    35821 gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc
    35881 tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc
    35941 acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg
    36001 gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt
    36061 ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa
    36121 aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc
    36181 aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg
    36241 aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta
    36301 agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa
    36361 gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta
    36421 gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg
    36481 gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc
    36541 ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc
    36601 gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac
    36661 ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat
    36721 agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa
    36781 gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa
    36841 gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat
    36901 tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc
    36961 cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga
    37021 tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa
    37081 atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga
    37141 tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt
    37201 tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga
    37261 ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca
    37321 tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag
    37381 gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat
    37441 caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa
    37501 tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata
    37561 aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt
    37621 atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg
    37681 gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct
    37741 tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga
    37801 gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat
    37861 gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat
    37921 tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt
    37981 attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag
    38041 tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct
    38101 gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc
    38161 tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat
    38221 catctctggc tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat
    38281 catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa
    38341 tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt
    38401 ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg
    38461 aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat
    38521 tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt
    38581 aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa
    38641 cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt
    38701 tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca
    38761 acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat
    38821 ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct
    38881 ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa
    38941 caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca
    39001 gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta
    39061 tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg
    39121 gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca
    39181 acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa
    39241 tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag
    39301 gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg
    39361 acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg
    39421 gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg
    39481 aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata
    39541 tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta
    39601 aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact
    39661 ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa
    39721 ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt
    39781 taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg
    39841 ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt
    39901 tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc
    39961 atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg
    40021 atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac
    40081 taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact
    40141 atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg
    40201 aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag
    40261 ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt
    40321 tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa
    40381 gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat
    40441 ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc
    40501 gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta
    40561 cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg
    40621 ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac
    40681 ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa
    40741 atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca
    40801 caacttctcg tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt
    40861 caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat
    40921 gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca
    40981 cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa
    41041 tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat
    41101 ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac
    41161 acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga
    41221 tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt
    41281 gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac
    41341 gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca
    41401 tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat
    41461 cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt
    41521 ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca
    41581 agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt
    41641 tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg
    41701 aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg
    41761 ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg
    41821 atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga
    41881 ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc
    41941 gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag
    42001 atatccaaga attgttctta gattctggat taggaaaaga atcgttctca atcatttctc
    42061 aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag
    42121 aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg
    42181 aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa
    42241 ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga
    42301 ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca
    42361 acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc
    42421 aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga
    42481 tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac
    42541 aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa
    42601 cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga
    42661 tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa
    42721 cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag
    42781 atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag
    42841 aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag
    42901 tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc
    42961 agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga
    43021 aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag
    43081 tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt
    43141 tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag
    43201 cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa acagctttag
    43261 gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt
    43321 atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa
    43381 gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg
    43441 ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa
    43501 cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc
    43561 aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg
    43621 gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt
    43681 tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg
    43741 agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg
    43801 aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac
    43861 ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt
    43921 ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat
    43981 cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg
    44041 aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct
    44101 tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag
    44161 aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt
    44221 ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc
    44281 gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg
    44341 agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt
    44401 cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt
    44461 tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag
    44521 aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa
    44581 aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata
    44641 cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga
    44701 tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc
    44761 aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga
    44821 cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa
    44881 aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc
    44941 tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag
    45001 cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca
    45061 cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg
    45121 gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca
    45181 atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc
    45241 aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca
    45301 agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt
    45361 agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat
    45421 cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt
    45481 gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact
    45541 agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt
    45601 gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga
    45661 tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat
    45721 caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct
    45781 tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga
    45841 tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat
    45901 gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc
    45961 agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt
    46021 tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg
    46081 gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga
    46141 cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg
    46201 aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag
    46261 cgatacgaat taccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg
    46321 cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca
    46381 atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg
    46441 ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag
    46501 gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag
    46561 ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag
    46621 cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag
    46681 ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa
    46741 aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg
    46801 caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga
    46861 ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc
    46921 gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg
    46981 agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat
    47041 aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg
    47101 tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc
    47161 aaacaatttc gttctttttc ttagactttt tgattataat tagattcata tgaattggag
    47221 gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg
    47281 accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag
    47341 caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag
    47401 attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat
    47461 ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga
    47521 tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa
    47581 aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc
    47641 gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa
    47701 cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt
    47761 tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt
    47821 caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca
    47881 gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt
    47941 ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg
    48001 agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa
    48061 caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg
    48121 gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag
    48181 aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag
    48241 cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag
    48301 gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag
    48361 cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac
    48421 agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc
    48481 atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta
    48541 tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt
    48601 tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg
    48661 acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag
    48721 aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag
    48781 cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt
    48841 taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca
    48901 ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc
    48961 gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta
    49021 ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc
    49081 gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga
    49141 tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc
    49201 taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt
    49261 ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg
    49321 gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg
    49381 ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa
    49441 aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga
    49501 aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg
    49561 taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt
    49621 tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat
    49681 cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc
    49741 agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata
    49801 ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt
    49861 tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat
    49921 actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac
    49981 taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa
    50041 aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc aataatacct
    50101 aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat
    50161 cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta
    50221 ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc
    50281 agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt
    50341 tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa
    50401 ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag
    50461 tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct
    50521 ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc
    50581 aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt
    50641 tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg
    50701 cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg
    50761 tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc
    50821 tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg
    50881 cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg
    50941 tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta
    51001 gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac
    51061 cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg
    51121 gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta
    51181 tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca
    51241 ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca
    51301 atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag
    51361 tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt
    51421 cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta
    51481 ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca
    51541 gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat
    51601 ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt
    51661 cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat
    51721 cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac
    51781 acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg
    51841 gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc
    51901 ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt
    51961 ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc
    52021 gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt
    52081 atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag
    52141 atgtacagtt aacgattcct gaaaagaaat tgactgcttt tatcggtcca aatggtgctg
    52201 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
    52261 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
    52321 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
    52381 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
    52441 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
    52501 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
    52561 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
    52621 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
    52681 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
    52741 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
    52801 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
    52861 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
    52921 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
    52981 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
    53041 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
    53101 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
    53161 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
    53221 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
    53281 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
    53341 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
    53401 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
    53461 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
    53521 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
    53581 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
    53641 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
    53701 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
    53761 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
    53821 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
    53881 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
    53941 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
    54001 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
    54061 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
    54121 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
    54181 tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
    54241 ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc
    54301 ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag
    54361 cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg
    54421 ttttaataat tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa
    54481 tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt
    54541 atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc
    54601 ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt
    54661 tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct
    54721 ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag
    54781 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
    54841 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
    54901 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
    54961 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
    55021 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
    55081 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
    55141 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
    55201 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
    55261 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
    55321 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
    55381 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
    55441 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
    55501 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
    55561 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
    55621 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
    55681 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
    55741 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
    55801 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
    55861 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
    55921 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
    55981 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
    56041 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
    56101 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
    56161 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
    56221 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
    56281 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
    56341 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg
    56401 cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata
    56461 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca
    56521 ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag cagtatttct
    56581 agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt
    56641 tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc
    56701 gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa
    56761 agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc
    56821 atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca
    56881 gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag
    56941 aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg
    57001 gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg
    57061 gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat
    57121 gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca
    57181 gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa
    57241 gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta
    57301 acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg
    57361 gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt
    57421 tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca
    57481 ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt
    57541 catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg
    57601 atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg
    57661 aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat catagggtac
    57721 tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact
    57781 tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt
    57841 atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga
    57901 ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc
    57961 gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta
    58021 ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt
    58081 agtttactct cggattttac atttaaatct tatttatact taggaaatca acgagataga
    58141 gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt
    58201 caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct
    58261 tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat
    58321 cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg
    58381 cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca
    58441 tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt
    58501 cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga tctcattagg
    58561 cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa
    58621 gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa
    58681 gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc
    58741 agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc
    58801 gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat
    58861 gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt
    58921 gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa
    58981 gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa
    59041 ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa
    59101 gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat
    59161 aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa
    59221 ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat
    59281 ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta
    59341 agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt
    59401 tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa
    59461 gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt
    59521 ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat
    59581 cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa
    59641 ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa
    59701 ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat
    59761 tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac
    59821 aattatttat aacactccta gttattttag gggcactttt taacaatata tcagtgtcag
    59881 ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa
    59941 aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga
    60001 gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt
    60061 caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg
    60121 atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa
    60181 ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga
    60241 tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat
    60301 cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag
    60361 ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca
    60421 tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa
    60481 aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga
    60541 ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag
    60601 gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga
    60661 gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta
    60721 caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca
    60781 ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta ttatgcatac
    60841 ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat aacaaatagt
    60901 aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa tataccattt
    60961 tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt gatcgcgaga
    61021 aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg tatattatac
    61081 aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat ccccagctag
    61141 ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg aattatgagt
    61201 tttttttaag caaagaagat cacagaagtt attcattact taaatattta gaagcaagct
    61261 caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg acatttctct
    61321 tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta gagaaaaatt
    61381 tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa tgttctagca
    61441 aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta ttggctttct
    61501 ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg agttattctg
    61561 tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa attttttttg
    61621 aaaagagaaa gtt
//
LOCUS       DACCPY010000005        59394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCPY010000005 DACCPY010000000
VERSION     DACCPY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.005"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IVO69_000265"
     CDS             complement(59..187)
                     /locus_tag="IVO69_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7399075.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IVO69_000266"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IVO69_000266"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7399076.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IVO69_000267"
     CDS             complement(1674..2729)
                     /locus_tag="IVO69_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7399077.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IVO69_000268"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IVO69_000268"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7399078.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IVO69_000269"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IVO69_000269"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7399079.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IVO69_000270"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IVO69_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7399080.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IVO69_000271"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IVO69_000271"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7399081.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IVO69_000272"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IVO69_000272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7399082.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IVO69_000273"
     CDS             10491..12050
                     /locus_tag="IVO69_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7399083.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IVO69_000274"
     CDS             complement(12161..14092)
                     /locus_tag="IVO69_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7399084.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IVO69_000275"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IVO69_000275"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7399085.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IVO69_000276"
     CDS             complement(15021..15773)
                     /locus_tag="IVO69_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7399086.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IVO69_000277"
     CDS             complement(15932..16141)
                     /locus_tag="IVO69_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399087.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IVO69_000278"
     CDS             complement(16152..17480)
                     /locus_tag="IVO69_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399088.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IVO69_000279"
     CDS             complement(17845..18432)
                     /locus_tag="IVO69_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7399089.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IVO69_000280"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IVO69_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7399090.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IVO69_000281"
     CDS             complement(20012..21301)
                     /locus_tag="IVO69_000281"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7399091.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IVO69_000282"
     CDS             complement(21455..22387)
                     /locus_tag="IVO69_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399092.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IVO69_000283"
     CDS             complement(22544..23203)
                     /locus_tag="IVO69_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7399093.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IVO69_000284"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IVO69_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7399094.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IVO69_000285"
     CDS             complement(24244..24534)
                     /locus_tag="IVO69_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399095.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IVO69_000286"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IVO69_000286"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7399096.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IVO69_000287"
     CDS             complement(26821..28176)
                     /locus_tag="IVO69_000287"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7399097.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IVO69_000288"
     CDS             complement(28200..29369)
                     /locus_tag="IVO69_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399098.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IVO69_000289"
     CDS             complement(29366..30253)
                     /locus_tag="IVO69_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7399099.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IVO69_000290"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IVO69_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7399100.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IVO69_000291"
     CDS             34320..34841
                     /locus_tag="IVO69_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7399101.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IVO69_000292"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IVO69_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IVO69_000293"
     CDS             complement(36284..36940)
                     /locus_tag="IVO69_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399102.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IVO69_000294"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IVO69_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7399103.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IVO69_000295"
     CDS             complement(38477..39475)
                     /locus_tag="IVO69_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7399104.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IVO69_000296"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IVO69_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7399105.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IVO69_000297"
     CDS             complement(40491..41222)
                     /locus_tag="IVO69_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7399106.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IVO69_000298"
     CDS             complement(41226..42491)
                     /locus_tag="IVO69_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7399107.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IVO69_000299"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IVO69_000299"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7399108.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IVO69_000300"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IVO69_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7399109.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IVO69_000301"
     CDS             47833..49335
                     /locus_tag="IVO69_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7399110.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IVO69_000302"
     CDS             49337..50074
                     /locus_tag="IVO69_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7399111.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IVO69_000303"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IVO69_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7399112.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IVO69_000304"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IVO69_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7399113.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IVO69_000305"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IVO69_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7399114.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IVO69_000306"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IVO69_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7399115.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IVO69_000307"
     CDS             complement(55574..56740)
                     /locus_tag="IVO69_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7399116.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IVO69_000308"
     CDS             complement(56756..56983)
                     /locus_tag="IVO69_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7399117.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IVO69_000309"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IVO69_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="IVO69_000310"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="IVO69_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16402 a        12848 c        10314 g        19830 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt tttt
//
LOCUS       DACCPY010000006        56387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCPY010000006 DACCPY010000000
VERSION     DACCPY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.006"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IVO69_000311"
     CDS             330..1175
                     /locus_tag="IVO69_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7399118.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IVO69_000312"
     CDS             1456..2676
                     /locus_tag="IVO69_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7399119.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IVO69_000313"
     CDS             2673..5393
                     /locus_tag="IVO69_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7399120.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IVO69_000314"
     CDS             5377..6321
                     /locus_tag="IVO69_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7399121.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IVO69_000315"
     CDS             complement(6390..7400)
                     /locus_tag="IVO69_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7399122.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IVO69_000316"
     CDS             complement(7580..7924)
                     /locus_tag="IVO69_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7399123.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IVO69_000317"
     CDS             complement(7917..8351)
                     /locus_tag="IVO69_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7399124.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IVO69_000318"
     CDS             8489..9226
                     /locus_tag="IVO69_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399125.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IVO69_000319"
     CDS             9219..10430
                     /locus_tag="IVO69_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399126.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IVO69_000320"
     CDS             10637..11416
                     /locus_tag="IVO69_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7399127.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IVO69_000321"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IVO69_000321"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7399128.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVO69_000322"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVO69_000322"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7399129.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IVO69_000323"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IVO69_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7399130.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IVO69_000324"
     CDS             15018..16067
                     /locus_tag="IVO69_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7399131.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IVO69_000325"
     CDS             16064..16342
                     /locus_tag="IVO69_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7399132.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IVO69_000326"
     CDS             16317..16787
                     /locus_tag="IVO69_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7399133.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IVO69_000327"
     CDS             16804..17106
                     /locus_tag="IVO69_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399134.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IVO69_000328"
     CDS             17123..17494
                     /locus_tag="IVO69_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7399135.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IVO69_000329"
     CDS             17466..17825
                     /locus_tag="IVO69_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399136.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IVO69_000330"
     CDS             17997..19004
                     /locus_tag="IVO69_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7399137.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IVO69_000331"
     CDS             19029..20213
                     /locus_tag="IVO69_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7399138.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IVO69_000332"
     CDS             complement(20650..22233)
                     /locus_tag="IVO69_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7399139.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IVO69_000333"
     CDS             complement(22811..22954)
                     /locus_tag="IVO69_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7399140.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IVO69_000334"
     CDS             complement(23177..23656)
                     /locus_tag="IVO69_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7399141.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IVO69_000335"
     CDS             complement(23729..24013)
                     /locus_tag="IVO69_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399142.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IVO69_000336"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVO69_000336"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IVO69_000337"
     CDS             complement(24357..25643)
                     /locus_tag="IVO69_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7399143.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IVO69_000338"
     CDS             25781..26452
                     /locus_tag="IVO69_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7399144.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IVO69_000339"
     CDS             26418..26906
                     /locus_tag="IVO69_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7399145.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IVO69_000340"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IVO69_000340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7399146.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IVO69_000341"
     CDS             27867..28631
                     /locus_tag="IVO69_000341"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7399147.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IVO69_000342"
     CDS             28864..31077
                     /locus_tag="IVO69_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7399148.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IVO69_000343"
     CDS             31088..31534
                     /locus_tag="IVO69_000343"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7399149.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IVO69_000344"
     CDS             31705..32964
                     /locus_tag="IVO69_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7399150.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IVO69_000345"
     CDS             complement(33053..34000)
                     /locus_tag="IVO69_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7399151.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IVO69_000346"
     CDS             34394..35686
                     /locus_tag="IVO69_000346"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7399152.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IVO69_000347"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IVO69_000347"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7399153.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IVO69_000348"
     CDS             37579..38454
                     /locus_tag="IVO69_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7399154.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IVO69_000349"
     CDS             38720..40603
                     /locus_tag="IVO69_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7399155.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IVO69_000350"
     CDS             complement(40678..40962)
                     /locus_tag="IVO69_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7399156.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IVO69_000351"
     CDS             complement(40952..41686)
                     /locus_tag="IVO69_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7399157.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IVO69_000352"
     CDS             41815..42438
                     /locus_tag="IVO69_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7399158.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IVO69_000353"
     CDS             42630..43751
                     /locus_tag="IVO69_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7399159.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IVO69_000354"
     CDS             43758..46886
                     /locus_tag="IVO69_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7399160.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IVO69_000355"
     CDS             46905..50099
                     /locus_tag="IVO69_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7399161.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IVO69_000356"
     CDS             complement(50245..50952)
                     /locus_tag="IVO69_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7399162.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IVO69_000357"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IVO69_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7399163.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IVO69_000358"
     CDS             52447..53100
                     /locus_tag="IVO69_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7399164.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IVO69_000359"
     CDS             53326..54324
                     /locus_tag="IVO69_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7399165.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="IVO69_000360"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="IVO69_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7399166.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18554 a         9728 c        11686 g        16419 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt atttttt
//
LOCUS       DACCPY010000007        54287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCPY010000007 DACCPY010000000
VERSION     DACCPY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.007"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IVO69_000361"
     CDS             complement(543..2048)
                     /locus_tag="IVO69_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7399167.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVO69_000362"
     CDS             complement(2045..2731)
                     /locus_tag="IVO69_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7399168.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO69_000363"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO69_000363"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7399169.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO69_000364"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO69_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7399170.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IVO69_000365"
     CDS             complement(4752..5321)
                     /locus_tag="IVO69_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7399171.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IVO69_000366"
     CDS             5507..6409
                     /locus_tag="IVO69_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7399172.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVO69_000367"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVO69_000367"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7399173.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVO69_000368"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVO69_000368"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7399174.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVO69_000369"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVO69_000369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7399175.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IVO69_000370"
     CDS             12977..13162
                     /locus_tag="IVO69_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7399176.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IVO69_000371"
     CDS             complement(13224..14420)
                     /locus_tag="IVO69_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7399177.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IVO69_000372"
     CDS             complement(14590..16026)
                     /locus_tag="IVO69_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7399178.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IVO69_000373"
     CDS             16319..17128
                     /locus_tag="IVO69_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7399179.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(17314..19467)
                     /locus_tag="IVO69_000374"
     CDS             complement(17314..19467)
                     /locus_tag="IVO69_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7399180.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(19761..20609)
                     /locus_tag="IVO69_000375"
     CDS             complement(19761..20609)
                     /locus_tag="IVO69_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7399181.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(20833..22770)
                     /gene="thrS"
                     /locus_tag="IVO69_000376"
     CDS             complement(20833..22770)
                     /gene="thrS"
                     /locus_tag="IVO69_000376"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7399182.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYGT
                     AFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTIE
                     RYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMNC
                     PHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPDQ
                     IKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAMD
                     ELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGEN
                     THRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIKE
                     RLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETAV
                     EDLNIFIDAMEAEVKNYSREN"
     gene            complement(23107..23286)
                     /locus_tag="IVO69_000377"
     CDS             complement(23107..23286)
                     /locus_tag="IVO69_000377"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7399183.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(23333..25156)
                     /gene="cadA"
                     /locus_tag="IVO69_000378"
     CDS             complement(23333..25156)
                     /gene="cadA"
                     /locus_tag="IVO69_000378"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7399184.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            25434..27713
                     /locus_tag="IVO69_000379"
     CDS             25434..27713
                     /locus_tag="IVO69_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7399185.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(27806..28453)
                     /locus_tag="IVO69_000380"
     CDS             complement(27806..28453)
                     /locus_tag="IVO69_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399186.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(28632..29351)
                     /locus_tag="IVO69_000381"
     CDS             complement(28632..29351)
                     /locus_tag="IVO69_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7399187.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(29482..30633)
                     /locus_tag="IVO69_000382"
     CDS             complement(29482..30633)
                     /locus_tag="IVO69_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7399188.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(30850..31590)
                     /locus_tag="IVO69_000383"
     CDS             complement(30850..31590)
                     /locus_tag="IVO69_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7399189.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(31594..31917)
                     /gene="rsfS"
                     /locus_tag="IVO69_000384"
     CDS             complement(31594..31917)
                     /gene="rsfS"
                     /locus_tag="IVO69_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7399190.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(31974..32567)
                     /locus_tag="IVO69_000385"
     CDS             complement(31974..32567)
                     /locus_tag="IVO69_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7399191.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(32551..33198)
                     /locus_tag="IVO69_000386"
     CDS             complement(32551..33198)
                     /locus_tag="IVO69_000386"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7399192.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(33211..33522)
                     /gene="yhbY"
                     /locus_tag="IVO69_000387"
     CDS             complement(33211..33522)
                     /gene="yhbY"
                     /locus_tag="IVO69_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7399193.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(33538..34647)
                     /gene="yqeH"
                     /locus_tag="IVO69_000388"
     CDS             complement(33538..34647)
                     /gene="yqeH"
                     /locus_tag="IVO69_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7399194.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(34644..35171)
                     /locus_tag="IVO69_000389"
     CDS             complement(34644..35171)
                     /locus_tag="IVO69_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7399195.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(35351..36136)
                     /locus_tag="IVO69_000390"
     CDS             complement(35351..36136)
                     /locus_tag="IVO69_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7399196.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(36129..36998)
                     /locus_tag="IVO69_000391"
     CDS             complement(36129..36998)
                     /locus_tag="IVO69_000391"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7399197.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(36999..38378)
                     /locus_tag="IVO69_000392"
     CDS             complement(36999..38378)
                     /locus_tag="IVO69_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7399198.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(38386..38805)
                     /gene="fabZ"
                     /locus_tag="IVO69_000393"
     CDS             complement(38386..38805)
                     /gene="fabZ"
                     /locus_tag="IVO69_000393"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7399199.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(38830..39306)
                     /gene="accB"
                     /locus_tag="IVO69_000394"
     CDS             complement(38830..39306)
                     /gene="accB"
                     /locus_tag="IVO69_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7399200.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(39309..40544)
                     /gene="fabF"
                     /locus_tag="IVO69_000395"
     CDS             complement(39309..40544)
                     /gene="fabF"
                     /locus_tag="IVO69_000395"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7399201.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAIS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(40563..41300)
                     /gene="fabG"
                     /locus_tag="IVO69_000396"
     CDS             complement(40563..41300)
                     /gene="fabG"
                     /locus_tag="IVO69_000396"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7399202.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(41312..42229)
                     /gene="fabD"
                     /locus_tag="IVO69_000397"
     CDS             complement(41312..42229)
                     /gene="fabD"
                     /locus_tag="IVO69_000397"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7399203.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(42336..42560)
                     /locus_tag="IVO69_000398"
     CDS             complement(42336..42560)
                     /locus_tag="IVO69_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7399204.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(42613..43575)
                     /locus_tag="IVO69_000399"
     CDS             complement(42613..43575)
                     /locus_tag="IVO69_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7399205.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(43585..44025)
                     /locus_tag="IVO69_000400"
     CDS             complement(43585..44025)
                     /locus_tag="IVO69_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7399206.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            44331..45296
                     /locus_tag="IVO69_000401"
     CDS             44331..45296
                     /locus_tag="IVO69_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7399207.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(45353..45583)
                     /locus_tag="IVO69_000402"
     CDS             complement(45353..45583)
                     /locus_tag="IVO69_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7399208.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(45669..46556)
                     /locus_tag="IVO69_000403"
     CDS             complement(45669..46556)
                     /locus_tag="IVO69_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7399209.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(46645..47868)
                     /locus_tag="IVO69_000404"
     CDS             complement(46645..47868)
                     /locus_tag="IVO69_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7399210.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(47885..48964)
                     /locus_tag="IVO69_000405"
     CDS             complement(47885..48964)
                     /locus_tag="IVO69_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7399211.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(49095..50822)
                     /gene="ptsP"
                     /locus_tag="IVO69_000406"
     CDS             complement(49095..50822)
                     /gene="ptsP"
                     /locus_tag="IVO69_000406"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7399212.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(50822..51088)
                     /locus_tag="IVO69_000407"
     CDS             complement(50822..51088)
                     /locus_tag="IVO69_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7399213.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            51567..53801
                     /locus_tag="IVO69_000408"
     CDS             51567..53801
                     /locus_tag="IVO69_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7399214.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            53949..54269
                     /locus_tag="IVO69_000409"
     CDS             53949..54269
                     /locus_tag="IVO69_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7399215.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        15478 a        11624 c         9451 g        17734 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg
    17221 tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac
    17281 tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata
    17341 cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt
    17401 cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt
    17461 actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt
    17521 tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc
    17581 atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac
    17641 cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc
    17701 attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt
    17761 tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc
    17821 ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact tcttcatata
    17881 atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc
    17941 aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa
    18001 actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat
    18061 catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct
    18121 tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa
    18181 aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac
    18241 tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt
    18301 ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat
    18361 agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata
    18421 attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa
    18481 attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc
    18541 aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa
    18601 ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc
    18661 atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact
    18721 tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc
    18781 gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt
    18841 gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc
    18901 gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt
    18961 ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc
    19021 agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat
    19081 cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct
    19141 cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta
    19201 gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc
    19261 ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa
    19321 taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa
    19381 cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa
    19441 tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt
    19501 cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta
    19561 cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc
    19621 aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg
    19681 aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta
    19741 tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt
    19801 gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata
    19861 tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct
    19921 tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat
    19981 tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca
    20041 tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca
    20101 agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac
    20161 catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca
    20221 gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct
    20281 actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag
    20341 gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa
    20401 atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact
    20461 gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc
    20521 tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc
    20581 atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat
    20641 gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac
    20701 aatccgccca acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt
    20761 ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag
    20821 gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat
    20881 aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt
    20941 tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga
    21001 ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt
    21061 catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg
    21121 gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt
    21181 taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg
    21241 atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa
    21301 gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc
    21361 tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc
    21421 atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata
    21481 tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa
    21541 gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc
    21601 aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga
    21661 caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa
    21721 ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg
    21781 gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata
    21841 atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac
    21901 atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat
    21961 tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa
    22021 caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg
    22081 gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat
    22141 ttgattgttt gagtttccac gccaataagc acctgccact gataataatt taaatacttg
    22201 tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg
    22261 ataagcagta atcacttctt ctggcaattc actaatcagt tctactttgt acggatcact
    22321 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
    22381 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
    22441 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
    22501 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
    22561 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
    22621 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
    22681 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
    22741 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
    22801 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
    22861 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
    22921 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
    22981 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
    23041 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
    23101 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
    23161 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
    23221 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
    23281 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
    23341 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
    23401 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
    23461 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
    23521 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
    23581 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
    23641 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
    23701 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
    23761 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
    23821 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
    23881 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
    23941 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
    24001 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
    24061 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
    24121 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
    24181 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
    24241 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
    24301 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
    24361 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
    24421 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
    24481 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
    24541 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
    24601 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
    24661 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
    24721 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
    24781 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
    24841 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
    24901 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
    24961 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
    25021 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
    25081 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
    25141 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
    25201 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
    25261 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
    25321 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
    25381 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
    25441 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
    25501 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
    25561 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
    25621 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
    25681 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
    25741 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
    25801 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
    25861 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
    25921 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
    25981 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
    26041 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
    26101 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
    26161 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
    26221 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
    26281 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
    26341 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
    26401 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
    26461 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
    26521 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
    26581 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
    26641 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
    26701 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
    26761 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
    26821 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
    26881 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
    26941 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
    27001 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
    27061 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
    27121 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    27181 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    27241 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    27301 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    27361 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    27421 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    27481 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    27541 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    27601 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    27661 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    27721 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    27781 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    27841 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    27901 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    27961 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    28021 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    28081 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    28141 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    28201 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    28261 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    28321 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    28381 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    28441 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    28501 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    28561 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    28621 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    28681 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    28741 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    28801 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    28861 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    28921 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    28981 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    29041 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    29101 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    29161 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    29221 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    29281 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    29341 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    29401 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    29461 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    29521 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    29581 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    29641 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    29701 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    29761 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    29821 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    29881 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    29941 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    30001 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    30061 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    30121 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    30181 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    30241 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    30301 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    30361 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    30421 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    30481 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    30541 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    30601 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    30661 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    30721 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    30781 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    30841 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    30901 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    30961 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    31021 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    31081 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    31141 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    31201 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    31261 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    31321 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    31381 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    31441 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    31501 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    31561 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    31621 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    31681 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    31741 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    31801 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    31861 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    31921 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    31981 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    32041 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    32101 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    32161 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    32221 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    32281 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    32341 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    32401 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    32461 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    32521 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    32581 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    32641 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    32701 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    32761 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    32821 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    32881 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    32941 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    33001 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    33061 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    33121 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    33181 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    33241 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    33301 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    33361 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    33421 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    33481 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    33541 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    33601 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    33661 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    33721 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    33781 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    33841 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    33901 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    33961 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    34021 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    34081 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    34141 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    34201 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    34261 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    34321 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    34381 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    34441 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttgga tctcattata
    34501 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    34561 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    34621 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    34681 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    34741 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    34801 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    34861 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    34921 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    34981 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    35041 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    35101 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    35161 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    35221 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    35281 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    35341 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    35401 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    35461 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    35521 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    35581 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    35641 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    35701 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    35761 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    35821 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    35881 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    35941 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    36001 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    36061 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    36121 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    36181 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    36241 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    36301 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    36361 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    36421 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    36481 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    36541 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    36601 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    36661 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    36721 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    36781 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    36841 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    36901 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    36961 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    37021 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    37081 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    37141 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    37201 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    37261 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    37321 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    37381 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    37441 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    37501 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    37561 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    37621 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    37681 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    37741 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    37801 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    37861 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    37921 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    37981 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    38041 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    38101 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    38161 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    38221 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    38281 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    38341 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    38401 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    38461 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    38521 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    38581 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    38641 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    38701 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    38761 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    38821 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    38881 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    38941 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    39001 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    39061 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    39121 ttgttcagat atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    39181 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    39241 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    39301 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    39361 aatgaattac ttattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    39421 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    39481 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    39541 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    39601 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    39661 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    39721 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    39781 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    39841 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    39901 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    39961 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    40021 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    40081 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    40141 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    40201 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    40261 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    40321 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    40381 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    40441 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    40501 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    40561 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    40621 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    40681 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    40741 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    40801 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    40861 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    40921 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    40981 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    41041 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    41101 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    41161 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    41221 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    41281 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    41341 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    41401 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    41461 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    41521 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    41581 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    41641 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    41701 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    41761 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    41821 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggta gcttctgtca
    41881 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    41941 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    42001 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    42061 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    42121 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    42181 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    42241 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    42301 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    42361 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    42421 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    42481 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    42541 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    42601 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    42661 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    42721 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    42781 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    42841 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    42901 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    42961 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    43021 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    43081 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    43141 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    43201 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    43261 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    43321 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    43381 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    43441 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    43501 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    43561 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    43621 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    43681 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    43741 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    43801 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    43861 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    43921 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    43981 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    44041 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    44101 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    44161 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    44221 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    44281 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    44341 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    44401 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    44461 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    44521 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    44581 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    44641 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    44701 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    44761 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    44821 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    44881 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    44941 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    45001 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    45061 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    45121 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    45181 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    45241 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    45301 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    45361 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    45421 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    45481 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    45541 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    45601 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    45661 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    45721 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    45781 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    45841 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    45901 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    45961 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    46021 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    46081 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    46141 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    46201 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    46261 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    46321 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    46381 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    46441 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    46501 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    46561 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    46621 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    46681 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    46741 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    46801 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    46861 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    46921 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    46981 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    47041 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    47101 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    47161 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    47221 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    47281 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    47341 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    47401 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    47461 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    47521 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    47581 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    47641 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    47701 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    47761 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    47821 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    47881 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    47941 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    48001 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    48061 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    48121 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    48181 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    48241 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    48301 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    48361 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    48421 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    48481 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    48541 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    48601 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    48661 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    48721 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    48781 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    48841 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    48901 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    48961 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    49021 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    49081 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    49141 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    49201 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    49261 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    49321 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    49381 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    49441 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    49501 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    49561 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    49621 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    49681 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    49741 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    49801 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    49861 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    49921 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    49981 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    50041 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    50101 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    50161 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    50221 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    50281 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    50341 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    50401 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    50461 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    50521 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    50581 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    50641 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    50701 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    50761 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    50821 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    50881 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    50941 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    51001 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    51061 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    51121 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    51181 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    51241 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    51301 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    51361 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    51421 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    51481 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    51541 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    51601 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    51661 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    51721 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    51781 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    51841 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    51901 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    51961 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    52021 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    52081 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    52141 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    52201 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    52261 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    52321 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    52381 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    52441 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    52501 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    52561 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    52621 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    52681 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    52741 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    52801 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    52861 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    52921 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    52981 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    53041 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    53101 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    53161 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    53221 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    53281 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    53341 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    53401 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    53461 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    53521 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    53581 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    53641 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    53701 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    53761 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    53821 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    53881 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    53941 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    54001 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    54061 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    54121 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    54181 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    54241 atgaattagg cgctacatta gccgtgtaaa caaattttga acataat
//
LOCUS       DACCPY010000008        42295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCPY010000008 DACCPY010000000
VERSION     DACCPY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.008"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(342..923)
                     /locus_tag="IVO69_000410"
     CDS             complement(342..923)
                     /locus_tag="IVO69_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7399216.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1106..1732)
                     /locus_tag="IVO69_000411"
     CDS             complement(1106..1732)
                     /locus_tag="IVO69_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7399217.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1754..3082)
                     /locus_tag="IVO69_000412"
     CDS             complement(1754..3082)
                     /locus_tag="IVO69_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7399218.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEMTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3240..3641)
                     /locus_tag="IVO69_000413"
     CDS             complement(3240..3641)
                     /locus_tag="IVO69_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7399219.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3727..3933)
                     /locus_tag="IVO69_000414"
     CDS             complement(3727..3933)
                     /locus_tag="IVO69_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399220.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4028..5530)
                     /locus_tag="IVO69_000415"
     CDS             complement(4028..5530)
                     /locus_tag="IVO69_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7399221.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5548..6003)
                     /gene="argR"
                     /locus_tag="IVO69_000416"
     CDS             complement(5548..6003)
                     /gene="argR"
                     /locus_tag="IVO69_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7399222.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6101..6718)
                     /locus_tag="IVO69_000417"
     CDS             complement(6101..6718)
                     /locus_tag="IVO69_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399223.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6939..8312
                     /locus_tag="IVO69_000418"
     CDS             6939..8312
                     /locus_tag="IVO69_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7399224.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8387..9499
                     /locus_tag="IVO69_000419"
     CDS             8387..9499
                     /locus_tag="IVO69_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7399225.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9571..11208)
                     /locus_tag="IVO69_000420"
     CDS             complement(9571..11208)
                     /locus_tag="IVO69_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7399226.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11376..11984)
                     /locus_tag="IVO69_000421"
     CDS             complement(11376..11984)
                     /locus_tag="IVO69_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7399227.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPVAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DATYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12369..13520)
                     /gene="queG"
                     /locus_tag="IVO69_000422"
     CDS             complement(12369..13520)
                     /gene="queG"
                     /locus_tag="IVO69_000422"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7399228.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13539..14291)
                     /locus_tag="IVO69_000423"
     CDS             complement(13539..14291)
                     /locus_tag="IVO69_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399229.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIHFVQLKKEKLYYGLYSR"
     gene            complement(14431..15381)
                     /locus_tag="IVO69_000424"
     CDS             complement(14431..15381)
                     /locus_tag="IVO69_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7399230.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(15382..16221)
                     /locus_tag="IVO69_000425"
     CDS             complement(15382..16221)
                     /locus_tag="IVO69_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7399231.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(16218..16964)
                     /locus_tag="IVO69_000426"
     CDS             complement(16218..16964)
                     /locus_tag="IVO69_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7399232.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            17202..18572
                     /locus_tag="IVO69_000427"
     CDS             17202..18572
                     /locus_tag="IVO69_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399233.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18627..19523)
                     /locus_tag="IVO69_000428"
     CDS             complement(18627..19523)
                     /locus_tag="IVO69_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7399234.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19804..20481)
                     /locus_tag="IVO69_000429"
     CDS             complement(19804..20481)
                     /locus_tag="IVO69_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7399235.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20572..21477)
                     /locus_tag="IVO69_000430"
     CDS             complement(20572..21477)
                     /locus_tag="IVO69_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7399236.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21652..23106
                     /locus_tag="IVO69_000431"
     CDS             21652..23106
                     /locus_tag="IVO69_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7399237.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            23122..24153
                     /locus_tag="IVO69_000432"
     CDS             23122..24153
                     /locus_tag="IVO69_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7399238.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            24160..25347
                     /locus_tag="IVO69_000433"
     CDS             24160..25347
                     /locus_tag="IVO69_000433"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7399239.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25503..26885
                     /locus_tag="IVO69_000434"
     CDS             25503..26885
                     /locus_tag="IVO69_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7399240.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26857..27243
                     /locus_tag="IVO69_000435"
     CDS             26857..27243
                     /locus_tag="IVO69_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399241.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27443..28111)
                     /locus_tag="IVO69_000436"
     CDS             complement(27443..28111)
                     /locus_tag="IVO69_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7399242.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(28300..29097)
                     /locus_tag="IVO69_000437"
                     /pseudo
     CDS             complement(28300..29097)
                     /locus_tag="IVO69_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29378..30757)
                     /locus_tag="IVO69_000438"
     CDS             complement(29378..30757)
                     /locus_tag="IVO69_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7399243.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30932..32101)
                     /locus_tag="IVO69_000439"
     CDS             complement(30932..32101)
                     /locus_tag="IVO69_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7399244.1"
                     /translation="MMKLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(32076..33227)
                     /locus_tag="IVO69_000440"
     CDS             complement(32076..33227)
                     /locus_tag="IVO69_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7399245.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEIIQSI"
     gene            complement(33187..34548)
                     /locus_tag="IVO69_000441"
     CDS             complement(33187..34548)
                     /locus_tag="IVO69_000441"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7399246.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34545..35546)
                     /locus_tag="IVO69_000442"
     CDS             complement(34545..35546)
                     /locus_tag="IVO69_000442"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7399247.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35562..36257)
                     /gene="dapD"
                     /locus_tag="IVO69_000443"
     CDS             complement(35562..36257)
                     /gene="dapD"
                     /locus_tag="IVO69_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7399248.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(36263..36979)
                     /locus_tag="IVO69_000444"
     CDS             complement(36263..36979)
                     /locus_tag="IVO69_000444"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7399249.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36976..37857)
                     /locus_tag="IVO69_000445"
     CDS             complement(36976..37857)
                     /locus_tag="IVO69_000445"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7399250.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37860..38660)
                     /gene="dapF"
                     /locus_tag="IVO69_000446"
     CDS             complement(37860..38660)
                     /gene="dapF"
                     /locus_tag="IVO69_000446"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7399251.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38647..39906)
                     /gene="lysA"
                     /locus_tag="IVO69_000447"
     CDS             complement(38647..39906)
                     /gene="lysA"
                     /locus_tag="IVO69_000447"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7399252.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(40392..40955)
                     /gene="efp"
                     /locus_tag="IVO69_000448"
     CDS             complement(40392..40955)
                     /gene="efp"
                     /locus_tag="IVO69_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7399253.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            41382..>42295
                     /locus_tag="IVO69_000449"
     CDS             41382..>42295
                     /locus_tag="IVO69_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7399254.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
BASE COUNT        11720 a         8827 c         7648 g        14100 t
ORIGIN      
        1 tctatcatac actgcaacaa gaagttcgta ccaaaaaaac gctttcaatt tgtgtagaac
       61 taaaaaaatt gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctt
      121 ggtcacaagt cacaatattc aacaaacatg agaagctatt ttttgaaatt gttccttgtg
      181 acccgaagtg aaacggcagg cttacgacct ctgggtaaac ggtgttccag aagtagcttc
      241 ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aatgccttct
      301 tatttcttga agctgaccac ttctgtcaca acctctaatt attaaagaac cttttgcgaa
      361 gttaacaatc tttctttgat caattggaaa ttctgtctaa gtagtgatag atcttgtgtg
      421 actttcaaca ataagatact gccctggatt tcagcaatca acgctttggc ttgacggtct
      481 gcctctgcat cggacagttt ttcttcagaa agtgccagga tattttgttt cgtatacttg
      541 acccattgat tgactaattc tagtagcaac agacggtatt cttcatccac tgtactcaac
      601 tcaataatca aattacctac aggacagcca tgataagtga tctgtgtctc atgatcagcg
      661 aaaatccagt ccaacatttc ttctaatgct tcttctggct gttttccagc atttaagatg
      721 tcttcgaaca attctgtctg ccatacgtcc atatgcgcat ggatcaccgc tagacaaact
      781 tctttttttg aagaaaagta ataataaaat tgaccttttc ctgtatcaga agctgctaga
      841 atatctcgga ttgacgtttc atgatagcct tttttataaa taagatcttt ggtcgcttcc
      901 aagatctttt cctttgtttt caatgatatt ctcctttttc atttctcata gtcctatatt
      961 atacagtaaa ttcatcattt tttcttctta atcgtttaaa aaagactgtg ccaaattatc
     1021 tgacacagcc ctcattataa aactgttctc atatagctag atgttcattg attcaagaac
     1081 tgcgaggaca aaagcaattc ctatcttatg atagttgctc taatacttcc tctgttgtca
     1141 tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc atccattgct tcttgaccag
     1201 gagcaccaat tgcatctgtc gctaccacaa cattgaatcc actctctacc aattcacgca
     1261 tatgggaatc gctacacaag tttccgttca ttccaccgag aattactgtg tcgattccat
     1321 tttttctcaa ttgaagcgct aagtcgttgc tttctggtcc aaagatttta tgaggagagc
     1381 tgatgatcgt attttcatcc aaatatggtt tcaattcttc gatgaaatca gctcctgaac
     1441 cttcttcaac tgattcatat tgacttttcc tagcaaacat ctgctgttca agcatcatgt
     1501 gttctcctgc accgccaaat tgccatttat ggtcatgtgg gtaaaaataa tgaggagaaa
     1561 taaagagttt gtatcctttt tcttttgctt gtttcaccat ttcgatcatg ttttcgatcg
     1621 tattgtattt ttctaagata tcttttgtca aaccaaagcc accgcctgct ggtttcaaga
     1681 actcgacttg cgggtcagtg agaactactg ctgtattttt attattgaac atggtggatt
     1741 ctccattcgt tatttatttt aatacatcat ttaaagcttc actcattgtt ggatgagaat
     1801 agatttgatc tctcaataac gtataaggaa gttttccatt catagctagc gaaatcaagt
     1861 tgatgacttc atgagaatct tccgcataga tacttgcccc tagaatcaag tcagtttcag
     1921 ggtcgactaa aactttgaat acacctttag gatcttctaa tacttgtgct ttagggacac
     1981 cgcttgtcat atacttgaat aatttgtaat tgacgccttg tgctttggct tgtttttctg
     2041 ttaaacctac attggataaa gtcggagtga taaatacact gtaaggcaca gctttccggt
     2101 tattcgttgt tcgtttattc tcgcccttca actgatccat aatgattcga taatcatcta
     2161 gagagatata agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa atgttgtctg
     2221 catctgtacg aaggtattca tccacagcga ttgctccgcg atctgttagt ttaacatctg
     2281 tattttctaa tccaagtgat gctgtacttg gttttcttcc agttgcagcc aaaacttttg
     2341 acgcttggat tgttacttct tggccatctt ctgtaaaggt gacggcagct gttgattctt
     2401 ggtctgctac ttgatctaca gatacaccta gatggaattc gattcccgca tttgtcatgt
     2461 cttctaaaat catttgcgcg atatcttcat cttcacgagg aagaaattca ggatgggcat
     2521 caagaacgac aactttagat ccgtattcat tgaacattga tgcaaattcc aagccaatat
     2581 atccagcccc gatgatcact aatttttccg gtaattgttc ctgatccatc gcttgcgtag
     2641 aatcaatcaa gtagcgactt gtattcaatc ctttgattgg taaaatgatt ggttcagaac
     2701 cagtattgat gaaaaaccgt tcgccttcaa caatcacttt ttcaccatta ttcgtcattt
     2761 cgatttgatg gttagaaaga aaacgtgctg taccgtccat cactgtccca gttgcctcat
     2821 cgctgatcat atgataattt ttggcgcgaa gcatgcccgt caatttttct ttccgagcaa
     2881 cagcctctgt aaagtcgatt cctctttctc cattgaaaat caatgattta gaaggaatac
     2941 agccgatgtt gatacatgtt ccgccataca tacgtgttga cttttcaacg actaaaaccg
     3001 attcgccttt tgaagctaac gccttcgcta aagtcttacc gccctttcca aaaccgatca
     3061 caatattttg atatttttcc atcctttttc cctcgcttct tttttaccta ccgattggta
     3121 caaaaatatg ataaccgata attgtctatt ctccaaatca tctgctttat ttgtcaaaaa
     3181 aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg ttcgatttcc tttattcttc
     3241 taaacaatcg ttacttctac atcgatattt cctctagttg ctttggagta agggcagaat
     3301 tcatgcgcat cttctgcaat tttttgggct tcttcttcgc tgatgccatc gatatgcact
     3361 tctaatacta caccgatctg atagccacca tcatctaaat tataaagaga gacacgtgct
     3421 tttactgtgg actctgctgt catctctgct tctttaatca tgtgctgtac tgcaccattg
     3481 aaacaagaag cataacctgc tgcaaataat tgttctggat ttgttccttc tgcatggatt
     3541 cctggaggag tgattttcat atgcatcgag ccatttggtg cctcgacttc tccattgcgc
     3601 ccgccatggt tgatcattgc tgtttcgtat gcttttttca ttgtactgct cctttcgatt
     3661 gaccatttgc tacttggcta taactatagt gtatgacaag taaggcgctt tctacaaaaa
     3721 ttctgcttat tcgaatacca gaatgccgtc ttctactatt tttccaaaaa acaaaaaaat
     3781 actactcgtg aacaacagcg tcatatttgt tccaaaataa atagcaatct tttctacata
     3841 atcttttata tgattgattt taagatacat atataccaga aagttgaccc cataaaccaa
     3901 tactgttaaa aaaataacca tattcgttac cattttttcc tccttttgct ggtctcccta
     3961 ccagcactcg atttaaccat accacttttt ttgccgtcca agcaactagc ctttcaaaaa
     4021 gactggttta ttctgctgtt aatgattcaa gatcagaaaa atgtccgaat aattttgcta
     4081 attttactaa cgccaacttg ttctttccga attcatcatt ttctatctgt aatactagta
     4141 acggctcatg taagcttaaa cggatcaaaa accagccgtt tccgtatggc tcttctagat
     4201 catatcgaat accttcttca ttttccgggt cgaagttcat ccctttttct tcttccaaca
     4261 gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg tggttcatcg ctgaacaatt
     4321 ttaatcggat ttcttgtact tctacgggct gtttcaactc tttgatttgt tcatctaatg
     4381 tgataccttt tctttttaat tctggcaaaa gcatcaggat ctttgcgatg acatatgcac
     4441 catcatccag aaaatagttt tccttgaatg cagcgtgtcc gcttgtttcg atggctagct
     4501 gtgtatcttt cccaagacgg ttaagttcta atgctttatt gattacattt cgatagccgg
     4561 aaatataacg aacttggatg cctccttttt cttcaatgaa tctttgtaga tgagaagaag
     4621 taggggaatt cgtcacgatt cttgttcctg gatgctcttt taatacgatt gtacttaata
     4681 ctgcgatcaa attatttcga ttgatcactg ttccggaagc actgacaact gcagaacgat
     4741 ccacatctgt atcgaaaatg atcccaagat ctgcttcatt tgctaaaaca gcctgcttga
     4801 tactttccat cgcttcttta ttatcaggat ttgggatatg attaggaaat cggccatctg
     4861 gttccaaaaa ttgggaacct gttgaatccg cacctaagct attcaatact ttttcagcaa
     4921 agaatccacc agcaccattt ccagcatcca cgatgatttt ccaacctttt agtggtttgt
     4981 catcttgtgt tcccatccct tttttgattt ttgtgactaa atcttgcgca taacgtgtca
     5041 aaaggtctgc tttttctacg cttccagttt gttcgctttt tattttttgc tgctgtgata
     5101 ggataaaagc aatatcttct ttttctgctc ctccttctga ggtaaagaat tttattccat
     5161 tataataata gggaagatgg ctagcagtca gcataaccgc agcatcgcac gagaactgtt
     5221 caaattgagt ggacataaac atagcaggcg tagtagctaa ttcgaaatcg atgacatgta
     5281 tcccttgttc tttcagactt tggatcaacg cctctttcag tgctggtccg cttatcctac
     5341 tgtcatgacc aatcccaatc gtccattttt tatttggatt ggttgacttc ttctttttct
     5401 ttatccagtt tgctagtccc cagccgatct tttttaccat gtctgtcgtc aagtttactt
     5461 catatccttc aactggtaat gcgattcctc gaatatccga tccattttgt agcgctttca
     5521 tatctttcat ataaaagtcc tcctcattta ttccctcatt ttttccagct tttctgcaaa
     5581 tcgctgtgct tcttcttctg agcggcaaat aataatgaat gtatctacac cggccatcgt
     5641 tccgacaact tcagggtaag ccacttcatc tagccaattc gttaccacat cggcaccatt
     5701 tcgttcagtc aatacgatga ccatgaactg aacagactgg atacgcaaaa cctcgcgtct
     5761 aattgctgta cgcaaacgtt cttcatttaa agaaacagat ggctggttaa ataatgtata
     5821 gcgtactatt ttgttttcat tttgactttt tactaacttc atttctcgaa tgtctcgaga
     5881 aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc cgcgcaagca gctcttcctg
     5941 tgtcccaata ggttgttctg taatgatctg gcggatcagt gcttgtcgct gtttcttttt
     6001 catattagac acctctcaat gttgattata gcaaaagata agtaatattt cttcatattt
     6061 tggtattttt tacttttatt ttttgttttc attcaggttg ctaacctttt tctattttaa
     6121 cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct tggatcgctg atgtaaatct
     6181 ctttgtgttc tttagaagct ctttcatagc cttcttttgc gataaatgct tctaatttct
     6241 caaacgttgc tggttcttcg tcatagggcc cgacgtgtaa gatttgggca caaagacctt
     6301 cttccatctt tttgaatact acttgttctt tcaagtcttc cggaagtttg ctagaacgct
     6361 gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg ctgtcggatc atcaattgat
     6421 aagaaaaatg ttcttttttc atcactggtt catgcaaata cttttcttgc agtccccatt
     6481 gtccttccag tggatagaca gtatacatct gataatcagg aatcagccaa ttttttttag
     6541 gcgacatgcg tattgcatag ctgactgcat atagacaggc tactcttcgt ttaaagtctt
     6601 cctgattcgg gtcgccgatc cctctgatgc taaagaatgg ttttttagga acctctagta
     6661 tttgcgggct ttttttcata ctatagctct tgtctttacg ccattcaatt ttttccattt
     6721 ttcttcctcc gcgaaattcc atttagttat ttattatcat acctcttttt gagtgacttg
     6781 gctaatcgtg ttgacactat ttttcagttt gtgctagaat aagttaagta aataaatcgt
     6841 acagataaca cgtttcggcg tgtcgagaga gaggcggttt ctatttttat aatagaaatc
     6901 actctctttt tttacgaaat tttttagagg tgatgtaaat gacaaaacaa aaacagctgc
     6961 ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt tgcgggcgta ttgatcgaga
     7021 cggccatgaa tgtaactttc ccaactctta tcagacagtt tgatttaacg actgcacaag
     7081 tccaatgggt aacaacgatt tatcttttga tgatttcgat cattgttccg ctgtctactt
     7141 atttgaatcg caattttagt ctcaaaaaac tctttttagc ttcaaatctg cttttcttgg
     7201 caggtataat gatcgatttc ttctctccta ccttcatggt cctattgttt ggtcgattgc
     7261 tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt ccatattatc ttgacatttg
     7321 cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac tttgacaacg gccattgctc
     7381 cagcaattgg tcccacttat ggaggcatta tgacttcaca ttttacatgg aatcatatct
     7441 tcctgtttct tgtgcccgtt ttgattcttt ctttgattat cggctggttt gctattcccg
     7501 agatggcagt acaaaaaagt ggttatttgg attggataag cgttttagga atttgtcttt
     7561 tgtttagtgg aagtctaaca tttttgagta cgttgggaac agtttccagc tggattgctt
     7621 taatcatcgg cggtattggc tggttccttt tttactatcg aagtaaaaaa gtggagcatc
     7681 cgctgatccg actgtctatt ctttcaaata aaacattccg cttgtttttg attggcttct
     7741 tagtttatca gtttttatta cttggtgtat cttttgtctt gccgaatttc cttcagatcg
     7801 ttcaaggaag tagtcctttt gtcgctggat tagcgatgct tcccggcgca gcggttggtg
     7861 ctttgctttc tcctttttcc gggaaaatgc tggatcaata tggtccgaag aagccaattt
     7921 ttgctgggct gattctttct ttgattggtt ggctagcatt attcctactc ataggacacg
     7981 cttcagtggg tgtattgatt gcttgtcatg tgttttatat gatcggtatc ggattatctt
     8041 acagcaatat gatgaccacc ggaatgaatg cactgaatga aaaactacaa ggagacggga
     8101 acgcaatgtt caataccttg cagcagtttt ctggcgctgt ggctacttca ttagtagcgg
     8161 tcattatcaa ttacgtccag catcatactt cccataacta tgaagtgtct actacattag
     8221 gatcaaaagc agcattaggc gtcttgttgc tgttgcttct cgtaagcttt gctcgattcg
     8281 cctattatct gttctttgct aaaaaagcgt agattccata tacggtaggt gttgcatctg
     8341 ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc ttttttatgg aaaaaattgc
     8401 cattatcggt ggcgggatca ttggtatgac cctagccagt tacttagata catcaaaatt
     8461 caatgttgct ttatttgatg aagggacagg acaggcaact aaagctagcg ctgggattat
     8521 ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat cgtctggcta aagacggcgc
     8581 tgcttttttc cctaaactta taaaagattt ccaacttgat caaacgattt acgcacaatc
     8641 agggacactt ttgcttcgac ctgaaaaaga tttagctgaa ttggctgttc ttgccgagaa
     8701 acgaaaagaa gaagcgccag aaattggaaa catcgaatgg ctttcacctg aacaaacatc
     8761 tcaagcactt ccgttattga aaccagcagt ttcactgcgt atcagtggtg gtggtcgtct
     8821 ggatggaaaa gcatatttag aacatcttgg tcagcgttta gcacaaaaac aaatggagca
     8881 gatccaggca aaagtcactt cgcataagaa gagcgattct tggctcatca agggaccgtt
     8941 tggagaatac acagctgatc gtgtaatttt gacacctggc ccggcactta agcctttatt
     9001 acaagaatta ggctatgctg cagatatacg tccgcaaaaa gggcagctct tagcatttca
     9061 gacatcttat aagatgagtc aagactggcc tgttgctatg cttgacggag aagctgattt
     9121 cattcctttc atggatggaa agatcctttt aggtgctacc catgaaaacg atcaaggctg
     9181 ggatttggct cctactagcg aagcttataa gggattgact aagagtacag aacagttctt
     9241 gactgatcca gatcagataa aaaactggcc atacgagttc cgtgtaggta cccgtgctta
     9301 tacgtcagat ttttccccat ttttcggcgt actgccagat gatccaagct tactggctgc
     9361 tagcggtcta ggctcttctg gcttgaccac aggtccttat atcggctatc tattagccaa
     9421 cttcttaaat actggtcatt ggcctacgga agattatcaa aaggacatct ccacctatat
     9481 tacgaaaaat ccatcataga aaaacagctt tttcaagtaa ggatcccgag cgatattgcg
     9541 catggattac ttcttgaaaa agctgttatt ttagttcaat ttctgaatca aattctccaa
     9601 actttctttt gcttcatctg gcaagagatc ttttcctatc tgagcttcaa tgaatcgtaa
     9661 acgaatatca agtcgttgtt tcaccagatt tttataggac gtatcctcaa aagaaacagg
     9721 aaaatctggt ataggtaaat agtggatgtc ttcaaatgga ccaaacgact tgaattctgc
     9781 cgtttgttgc acgattcttt caacgattga caatgtcgtt tcaggtttga tttttttatc
     9841 atcgaaatat ccagtattca ggacatagca ggctgtttgc tgtttcaaaa acaactctcg
     9901 aaaagcttga tagtcttccc taagaggata gacacggaaa ggattagcaa atggttcaat
     9961 gactagctca tctagctgtc tttttgtagt gatattttcc gcgtctgatc gttttgttgc
    10021 taatgttgca ccaaaagacg ctgctaaaac cgggtcattg atcttgataa ttggcggaag
    10081 cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt tcgctaagat gatcgactcg
    10141 gtcgggtgtc gcataacgtg atttgactgt ccgcccattt ccgtttcgta tatctcctgt
    10201 tactaggact ttttctcctt tacttgtaca tgtcacgccc acattctgac aagtaaggaa
    10261 agaagaaatg gccggatcat cagacggata gtcttgtgtt ttatcaaagt aagaaggttc
    10321 caaggcagta gcaaaaccat tttttcgaga aataacgaaa gcatcgtcat gcaagatcga
    10381 aacagagtga ccttccgtat gttttgctaa ggtaatcgtt gattttccag aaccggacaa
    10441 cccaaataca gccatcgtat aggtctggtc attactagca tattgtttca tcccaccgtg
    10501 gcaggcaata aaaccattgc gatttcccaa tgcccaagca agcgtcaaag tcgctttttt
    10561 taattctcca aagtaacgta atcctagcac agccgctgta ttatggagag catcaaacaa
    10621 aatgagtcca ttgggaaatg tttcatgtgt ccactcggga tcacaaaaga tataaagatc
    10681 tccctcatca aaggaaacag acttttgata agtctttaag atcttcgaat tcgctggctg
    10741 aaaattcagc aaataagaat acaaattgtt ctcaaatcct tctggaatca gcaaatgact
    10801 ttgcacagaa aattcttcag ataggccaac gattactttt gtatggtaaa actggcgttt
    10861 gcgactttga tacacagctt ctcttaaaat tttctcgtat ttgctttgtt ccttgtctaa
    10921 gtcctcaaag atccgccgag cacttgctgt tcttcccaca atgcttccat cattgaatac
    10981 tagtgtcctg acatcagctg gtaaatctag ttcttcagca tgtgagattg ttaaatctgt
    11041 gatgatcgtt cctggactcc cttttgctag ttgataagct gtcttcctgt caaaaacttc
    11101 atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat cgaatactgg ataacagtgg
    11161 atttccactt gtgatttcgt ttaattcgag actctctttt gtactcatgg ctcctcctca
    11221 ttccctaaac aaaaaaacgt tttctttatt atacattatt cgagttttta tttgtactgc
    11281 tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa aaccgagaac ttgaataaac
    11341 aagtttctcg gtttcatcat tttcctttta tcggcttagt accagccgtt tgctaaccag
    11401 aatgttttag cagcatccca tgaaccataa cgagatgtta catattgttc tgctacacgt
    11461 tcttgattgg cagctgaata gtcgccgttt aagtatgtag catctaattg ataacggccg
    11521 atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt ctttttgtgc gatccattct
    11581 tttgcagagc ttgaagttgg cgctgttgca gcaactgggg cagcaggagc tacttcaact
    11641 gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg tttcagttgt agatgctgta
    11701 ttttctgttg ttttgttttc tgttgttgct gaagaatcat ttgaagttgg gattgttaat
    11761 tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat ttttttcagc aattgcatcg
    11821 atcattgagt tgtctcctgc aaatttttgt gagatcttag ataatgaatc acctgatttt
    11881 actgtataaa cttcatcagc gtgagcattt gtagttccta caaacattgc agtagcacca
    11941 gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat tcatattaaa ctcctttttt
    12001 tctccgtttt ttattctttc gacgctatta actttatcat ttagaaatat ttcataggta
    12061 aaagaatcat gacatttaat gacaaaatga cgaattattt aatatttgta acatttaaac
    12121 aaatattttt ttgatacaaa accataaaat ccttgttgta taagcaaagt aatcgttttt
    12181 ctatgttttt ttatttttga atgaaaaacg cagattctgt aacatttact gcaataatcc
    12241 ataaaaaaaa agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa aatgtaaaaa
    12301 atacaaaaaa accagtaatt gttacaacaa ttactggaca aaaactttga ataatgttaa
    12361 ctattttttt attttaactt tctccgcaat aatttctggg catgttccaa ttcgtttata
    12421 acgacttctt ctgtttcttt agctaatatt tcatctaata catcaagcca tttttccggt
    12481 tcttttgttc ctagacgtcc aatagcccaa gctgctgttc ctctgatgac agggcggaca
    12541 tctgttaaac attctgaaat ctcaggcaat gccgttcgat cacctagatt agctaaagca
    12601 atcaatgcgt ttctctgcaa aggtttcttc ccacgccatg aaccagctaa atgcccaaat
    12661 tgctgtttga agtctttatt agacatcgaa agcattggct ttaattttgg atatacttcg
    12721 tctacttttg gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt gtaagggcag
    12781 actaactggc agatatcaca tccataaatg acatttcgca tttttttacg atattcttcc
    12841 ggcatcatcc cttttgtctg agtttgataa gacaaacatc gctgggcatt cattcttccg
    12901 tcaccaagca atgcttttgt cggacaagca gtcacgcagc gcatacagtc accgcaacca
    12961 aattctcctg gtttatcagg ctcaaataca atattagtaa cgatctcacc aagatagaca
    13021 aatgaaccga actcttcagt gatcaacaat ccgtttcgtc caataaatcc taaaccagct
    13081 cgctgtgcta cagcgacatc gatcagttct ccagtatcta cttgcggagc tagacgccat
    13141 tcctcttctt tttgccattt ttctgctctt gtttggatga attcgatcaa tcggttcaaa
    13201 cggtctctga gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa ctgcccgcgt
    13261 ttttcatccc taggcacttc ttcatggatc ctagtaggat acgcaagtgc aatggaaatg
    13321 atcgatttcg gctgatcaaa tgtcatttct ggataaagac gctcatcgat atttggatgt
    13381 tcaaagccag aagtatggcc agctgctttt tgttcttcca acgattcttt taatgaatca
    13441 aaaggttccg ccgtggtgaa cccgattttg tcaattccta atcgtttgct ttcttgtatg
    13501 atttcttctt ttaatgtcat ggtcttcctc gcttactgtt aacggctgta taacccataa
    13561 tatagttttt cttttttcag ctggacaaaa tggatctgct gatcggcaat cttcttttct
    13621 tccatccatt tttttcttgc agcaaatact tcccaataat ttgttgaaat taattttctt
    13681 ttgttatcta atgcattaaa aaaattcaat gaaactggtt ttgtacttaa cagttcatgg
    13741 agccttacct cttcccatgg cgaaaactca gacagatcgc gggaatacaa tacttcttca
    13801 tagagatttt tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg cctggcaatt
    13861 tctactagac gattataaaa catattcatt aatttgctgt tggcaataaa ttgactttta
    13921 taataggccg actggatctt tttgattact ccttctgctt tgatgactga aaataatgca
    13981 ggccatgctt tgacggttat ttcgtcgttt tgtttaacta atacgccagg tgtgaagccg
    14041 actggagaaa ggaacacaaa ataaccgata ccggtatttt catctaaagg ctgaaattca
    14101 tcaaacaacc atttgcctat gtacagctct tcatctggtt ctgttgtctc attttgattt
    14161 gctatgaata atttagctaa ttctgcttca agaaattgaa ggtcttgatc cttgtcggta
    14221 ttttcctgct ggctgatcaa tcgaaagact tcttctttaa tcgtatattt atctttacgt
    14281 gtaaaattca tagccacacc ttccttcctt actctaacta taacggaatt tcttcaaaaa
    14341 aggtatctgt acgccgaaaa acagcataat ttttttcata tctcttataa aaaatgcaag
    14401 gcctgctcct caagctagac cttgcacttt tcagctttct tttatttgtg acaaaccttc
    14461 atggatcgtt tcaatattcc atttcatcat ctgatagtaa gaatctcctt gttctccttc
    14521 ttttgctatg gaatcagtga acagtttatc ataaattttc aaacccgttt cttttgctac
    14581 ccgttccatg cttcttgagt ctacactggt ttccacgaat aaaaccggta cttcttttgc
    14641 tcttatctga tcaatgatcg ctttcatttg atcaggcgta ccttgacttt ctgtattgat
    14701 ctcccatata taagctgctg gcaaatcata tgctcgtgaa aaatacttga atgccccttc
    14761 acttgttact aataattttt tgttctcttc gatagaagca aaactttcct tcgcttgtgt
    14821 atctaattct tttagttttt ccacatatgc ttctgcattt tttttataga gtgtcgcatt
    14881 ttctgcatct ttttcagaga atatacgtgc gatttcctcc acatatttta ttccgttaga
    14941 taggtctagc caagcatggg gatctgcttt tgtatgttct tcaccgctag ttaaataaag
    15001 aggttctaca tttttgctaa ctgcaaaata atcttttcct tcttcttttt tagccgtttc
    15061 catcaagtta tcgaaccagc tgttacctgt ttcaagattc aaaccgttgt ataaaataac
    15121 atccgcatcg cttgcctttt tgatgtcttc tggtagtact tcatattcat gcggatctgt
    15181 tccgactggt acgatactgt ggatatctat ttgatctgtt ccgacctctt ttgccatgtc
    15241 cgccaagatc gaattggtag ccacgactga caacttttct ttacttcctt catccgcttg
    15301 gtcagaagcc tgctttccgc aaccggctaa taccaagaaa ctaactgcca agattaaaaa
    15361 gagtgatttt tttgttttca tctatctgct tcctttctga aataaaattc cttttgttgg
    15421 agaaaataaa aaagcaagga taaaaaatgc ggtacatgct aacacgatcg tagctcctga
    15481 tggccaattg taactataac tgaaaaacac accgacaact gcacttagca taccgattcc
    15541 tgcagctgta atgatcattt tcagtaaatg gttcgttagc aagtaagccg tggcggctgg
    15601 tgtgatcaac atggcaatca ccaagattgt tcctactgtc tgtaaactga ctacagccac
    15661 taatgtcaaa aagaacatca aaagataatg gatcagcgat gtgttcagcc cataagcttt
    15721 tgccattgtc ggatcgaatg atgtgatctt caattcttta taaaagaaaa agacaaatat
    15781 caagacgatt ccactaacga gtgctgtcag caacaaatcg ctttccctaa cagctaagac
    15841 attcccaaat aaaatatgat acaaatctgt cgcactctgg gcatatgaaa tcaagataat
    15901 ccctaatgca aaaaaggagc taaagacaat tccaatcgct gtatcatttt tcaatcggct
    15961 ccgctgagtc acgaaaccaa tcactgctgc tgttagcatc ccaaaagcag tggctccaaa
    16021 gatgtaacta aaaccaaaca tataagaaat ggctacacct ggaagaactg cgtgagagat
    16081 agcgtctccc atcaaagaca tccctcgtaa aataataaaa gaaccgatga ctccagaagc
    16141 aagtcctaca agcatggaag tgaccaaagc gtattgcagg aattgataat tcattaatcc
    16201 atcaataaag gattggatca ttttttctct cctcctttta ctaaatgtct gatgatctca
    16261 ccataggcca attgaaggtt cttcgatgta aagacttctt gtactggacc ggaagcgatc
    16321 agctgtttat ttaaaagaat aacctcatcg aaatattctt ctactttatg aagatcatga
    16381 tggacgatca aaatcgtttt tcctgaattc ttcaattcct gcaagatgtc aaagatcttt
    16441 cgttcactta acgcatcaat ccctacgaat ggttcatcta aaaagatcca ttctgctcct
    16501 tgggctagag ctcttgcaat aaaaactctc tgaagctgtc cacccgatag ttcgctgatc
    16561 tgtcttttgg catattcttc catccctact tttttcaaag cttgtcttgc acgttctttt
    16621 tcttttttcc caggtctttg tccaattcgt aaagatggat atgttcctaa aagtacaacg
    16681 cctaatacat caattggaaa ggaaagatcc aattcacttc gctgttccac gtaggctatt
    16741 tttttacgga ctgagtttac cggctgtcca gaaaaggtga ctttaccagt agtagctggt
    16801 atcaactcta gcaaagattt cataaatgtt gattttcctg caccgtttgg tccgataata
    16861 ccggtaattt tttgtggatt tatcgtcaag ttgatagttt tcaaaacagt tttcccttga
    16921 taagcggctg aaaggtcttg aattacaatg gccgtacgtt ccaaatctta cactcctttt
    16981 taggtttacc taacttttta tttaaatata gtttaactaa aatggaaagc attttcaagt
    17041 agaatgatga aaaagttttt ttcgaatttt cctcgcattt tatgaaaatt ttctgtagaa
    17101 tcacatcaag agttcaataa ggctaaccta ataatgaaaa acatgtgttg aaacgtttga
    17161 ttattttttc ttgttgactc aacaacgaaa ggagtttttg tatggaagaa gaaaagttaa
    17221 ctcgaagtct tagtgcccgt catatccaaa tgatcgcact aggtggaacg atcggtgtcg
    17281 gtctgtttat gggtgcctcc tcaaccatac gttggacagg gccctcagtg atgggagcct
    17341 atgcgattgc aggattcttt ttgtatttga ttatgcgtgc tttaggcgaa atgctttacg
    17401 ttgatccttc gactggttct tttgcaaatt acgcgagcga gtacatccat cctgtggctg
    17461 gctacttgac cgcttggagt aatatttttc aatatattgt cgtaggaata agtgaagtga
    17521 ttgctgtagg atcttatatg aattattggt ggcctgattt gccggcaatt attcccggaa
    17581 tcattgtcgt cttgtttctt atgctggcaa atcttatttc tgtcaaggcg tttggcgaat
    17641 tagaattttg gttttcgatc atcaaagtca taacgatcat tttgatgatc attgcaggtc
    17701 ttggggtaat cttatttgga tttggtaatc atggtcaggc agtagggatt tcaaatcttt
    17761 ggaaaaatgg tggattcttc actggaggcg tcaaaggatt tttctttgca ttatcgatcg
    17821 ttgttgcttc ctatcaagga atcgaactga tcggaatgac tgctggtgaa gcagagaatc
    17881 ctaaaaagac gatcattgaa gctgtccagt ccactatcgg tcgaatcttg attttttata
    17941 ttggtgctat ttttatcatt gtgagtatct atccgtggaa tcaattgagt gagattggtt
    18001 caccttttgt acaaactttt tctaaagtcg gcattacatt tgctgcagga ctgattaatt
    18061 ttgtcgtgat tacagcggca ttatcaggtt gcaattcagg gattttcagc gcaagtcgta
    18121 tggtctatac acttgccata aatggaaaga tgtctaaaaa atttctgaaa ctatctcgtc
    18181 acggtgcgcc tttttatcct gtcgtagcaa tctcaggtgg aatcttaatt ggtctgatct
    18241 taaattatct tctaccaatg ttcatcaacc agtcagaaaa tattttcgtt ttcgtataca
    18301 gctcaagtat tttgcctggt atggttcctt gggtagtgat tttgatcagc gagattcgct
    18361 tcaaaaagaa gcacgcagat aaaatgcatg atcatccttt taaaatgcca ctatctcctt
    18421 atacgaatta tgtgacactt gcttttttag ctatcgtatt gattttcatg tttatcaatc
    18481 ctgaaacgag aatctcattg attattggac tcgttttctt gctttacatg acgatccatt
    18541 actttgtacg cgaaaaaaga accggaaaat aaaaaaagaa cgaagcttca ccttagtgga
    18601 agcttcgttc ttttttattg cttttatcaa ttcaatttta gcgtctcacc gggagtcagg
    18661 aaataattct ccatgctcat cccattcagc gacaagatcg tttctactgg tacgccagtt
    18721 cgttcggcta tttgatttgg accttctcct tccaaaacta cggttgtcgc agcctgtgaa
    18781 gtcgcgctgt tggtattagt ttcagaggaa tctgttgtgt cttgtgttgt tcgagcttgc
    18841 atttcagaag cttctaccga ctctgctgtc tcagaatcag aagatgttgt gtcttgtgtg
    18901 cttgttgact ctgcttgaag agaggcgcta gtctgcaaat aaatatcgga aatcttagcg
    18961 acatgattcg tataattagc cgataattga ttgatcaaag tatctaagtc tacaccgtct
    19021 ttattttctg ctgcctgata cgctgcacgt aattttctga tcccttcgtc ggagattgct
    19081 tgaagctcac gtgctttttg gttagcaact gctgataaac cagctattcc ctcttgattt
    19141 ccctgaattt ctgtctggta ttcttcaatc aatgcaggcg tcttttcttc gatctttttc
    19201 gaataatcat ttacgattgc ttgaagttgc tctggcaaac catttgctgt tttttcttca
    19261 gaagtttgtg tctcttttgt tttagttgat gactcacttg tttgtttcgt ctctgttttc
    19321 tttgtcgttg tttccgttat ggtcgtactt ttcgcttctg acgaattttt tccatgaaaa
    19381 tatagacccg caactcctcc tgctatgatc aaaaacacgg caactgctgc cccaatcatg
    19441 atcatccgct tacgagctgc ctgtttttgc tgtcttcttc gggtcatccg aacattttct
    19501 ccccactcat tttcatcata catttttttc accaccaatt tttatgtata atagcgttat
    19561 caaacgcttc atattcttat tgtagcaaat aattgtcgct gtgcacattt ttatctttgt
    19621 tatttataag aaaatttcac cagttgagat gattccaaga ttgtccgtcg atttctcctt
    19681 gcagctgcat tgttcgttcg tctatctcag ccatcagttt ctttatttca tataagagcg
    19741 cacggtcttg atattctgat ttttcgctta attcatctaa ttgttggatc agccaatcat
    19801 ttgtcataat gaagttcctt tcatttcttg cagtgtaaag gtcaagtctt cgatctgctc
    19861 ttttgcctca gtcagtgctt ctacgatata tgcttgtttt tcagattctt cttcctgcat
    19921 tttctgattt tcctgtactc tttgaataaa ccagtcaatc cgctgagtca gcgatggttt
    19981 gccttccaat gagagatact gctgataagc aattagcttc tcccaacgtt tttcctggga
    20041 taaatacata aattgtgcta aaacgaatgg cggaagatag gtcatatgga atttatttgc
    20101 gattcgctga tacggcctca aaaattcgga aatcgtatat tcttcttttc ctccagcttg
    20161 gtattctttt tccgccacac ctgttgtcaa tacgattcct agttctttcc cttttaatac
    20221 atgtgctgct cgttccaaga cattatcttg ccaaattttt aaatgagcag gagaactata
    20281 ccagtaaaac ggaaactgga aaatgatccg atcatgctgg gccaagagct gtagttcagt
    20341 ctctatatct atttttccgt ctggataact tgattccaaa tgatgccaag tagctggtaa
    20401 accagcagaa gcttctttga aaaattgctg atttaaagat tcttcgatcg ttggatggct
    20461 aatgatgata agtgtacgca tcccttttcc tcctttttag tcttcgcttt acagtgtagc
    20521 ataaccgcca caagtttctt ttgccgatgc ttgtcatttt aaccagacta atcaggttgt
    20581 ccaagaaact gcggtttgtt tttccaactg ttttttcaag gtatccaaag caatattctt
    20641 aaaattttcg tttttcatac gtttacgata gttcgtagcc gtttctccgg tactcttttt
    20701 gaacattcgg ctaaatacga ctagactatt gaaccctact tgataggcaa tactttccac
    20761 ggattcatcc gtcagagaaa gaagaaacgc ccctcgattg atccgtaccc cgatcaaata
    20821 ttccttgata ctggtaccca tatgattttt gaatactcgc gacaaatggc tccgattgac
    20881 cgctaaatgc tgggcgattt cctcaacaga caatttttca gcataatgtg cctcgatgta
    20941 tttgactgtt tcaacagcta aagaggaatg aaccttttgt acaccaccgc tgacctttcc
    21001 gccatctttt aaaaggactg ataaaaaacg gtaggttaat gctgttaact gtaattcatt
    21061 ttccaaggtt tcttccgtat ctttcatgca ttctaaaata atgtcgatat atttttgtat
    21121 gtcagaagag accatcgtgt attggtcttc tctgaaaagc gactgccgaa tgatttcttc
    21181 tgcagcactc ccaccaaatg aaagccagca atacatccac ggatcttctc catctgataa
    21241 gtaaaacgtc gagtctcctg gacgaatcaa gaacagatca ccttttttca attggaattg
    21301 ttgatttttc acatgataag atcctttgcc ttccatcacg acatgcagga tgtaatcatt
    21361 gcgaatagca ggaccaaaag aatgaaacgg catcgtttta gagaaaccac aaaaatcaaa
    21421 atacaattct ttttctactt gttgatgttc cttttttaaa aaaatcgatt gctccacgtc
    21481 tatccctcct tactccttta gtataatgca acaaaaagtt aaataaaagt cacagaaata
    21541 tattaaaaag gaagcgattt cttgttaagt ttaagtcatt aaaagatctt tgttattcgt
    21601 tgtaaccggt ttacttagta ttatatattg aacaggagag gaatacagat tatgaaaagc
    21661 aaaatgatcc aacgtgtgtc ctacgcttta ggcgcttttg gacacgacac attctatgtt
    21721 acattaagca cttattttat ggtattcgtg acaagccaga tgttcgcagg aacagataag
    21781 gcaacgaatg caaaaatgat tgctacagtt acaagcttag ttgtaggtat ccgcttagtt
    21841 gaaattattt ttgatccttt gattggtggc atcatcgata atactcgtac aaaatacggt
    21901 aaattcaaac catggttagt tatcggcggc gttgttagtt ccgtactctt ggtagcaatc
    21961 tttaccaatt tctttggctt agctactcat aatacgactt tatttatcat attatttgca
    22021 atcgttttca tcttattgga tagtttttat tcatttaagg atatcgcttt ttggtcaatg
    22081 attccagctt tgagtacgga aacaaaggaa cgtgaaaaat ttgctacgtt tgctcgtttc
    22141 ggctcttctc taggtgcaaa tggtactact ctagtcgttg tccctatcgt tactttcttt
    22201 acctatcttg ttactcataa acatattgaa ggcgcttcag gctggttctg gttcggtgtg
    22261 attgctgctg ttatttcagg cggtactgcg attatcactg ccttgggcac aaaagaagtc
    22321 gatactgcga tccgtcagca aaatgaaaaa gctgggatca aagatgtgtt caaagcagtt
    22381 gctcaaaatg atcagttgat gtggcttgcc cttgcttata tcgcttatgc gattgccaat
    22441 gcagcaacaa ctgccgtatt attctacttc ttcaaatatg tacttggcca accaactgaa
    22501 ttttggatcg ttggtctagt tgctgcctta cttggtttag tagctgttcc actattccca
    22561 atgcttacac gcgtcatctc tcgtcgctac gtttaccttg gagggatcgc aatgatgatt
    22621 tcttcttact ttttctttac gatcgcaggt tcaagtttgc caatcgttat catcgggcta
    22681 gtattgttct acttccctca acaattgatt ttcttatccg ctttgatgac gatcacagac
    22741 tctgttgaat atggtcaatg gaaaaatggt atccgtaacg aagctgttac tctatcaatt
    22801 cgccctttac tagataaaat cgctggtgct ttgtctaacg ggatcgtagg gtttgtcgct
    22861 atcgcttctg ggatgactgg taatgctact gctgctgata tcactgctca tggcgtaacg
    22921 actttcaaag cttatgcatt ctatgctcct gctgctttga tgattttctc cgctattatt
    22981 tttgcttgga agattacatt gacagagaaa aaacatgcac aaatcgttga ggaattggaa
    23041 aacaaattgg ctccagctga tacaatggaa gatgaacttc acgatgcagt agttccttcc
    23101 cattaagaaa ggatcttgaa aatgaagatt acagaaaaag attttggcca aggttatcac
    23161 ttgataacct tggagaacaa aaacaagcta gcactttcta tctctgattt aggtgctaga
    23221 atcgtcagtt taaaatcaaa tgatcgagaa ttagttttgg gatttgacac cgctgaagaa
    23281 tatatcgaaa aagatccgta tatcggtgct tctatcggtc gcaccgcagg gagaatcgaa
    23341 aatggccgct tttctctcaa tggaaaaact taccaattag ccacagatcc aaaaactgga
    23401 cataatctgc atggtggtgc tcctggattt gaattaaaaa aatggtctta tgtcattttg
    23461 aatggcgaaa atgaagcttc tgtcattttt actactacaa gtcccgacgg agaacatggt
    23521 tttccaggta caatggatgt ggaaatccgc tatactttga caaaagataa tatctggcga
    23581 gtgacctctc gcggcaccag tgaccaagat actttattca atccgacaaa tcatgtttac
    23641 ttcaacttga cgggagatgc ttctcagtca atcgatcaac atgagctttg gctcaatagc
    23701 gaagcttatg ctcctttgcg aacagatagt attcctatag gggtaaaaga aaatgctgca
    23761 ggaagtgctt tcgattttca aatacctaaa aaattagcgt ccgtttttgc tagtgatttg
    23821 gatcaaaaaa atcttgttga cggaatcgat catccatttt tcttaaaaga aaccggcttg
    23881 ggtaaagaag ccgctcgctt gactagcccc gataaaagaa tccaagtagg tattgctaca
    23941 gatgcttcta gtgtggttat tttcactgca aatttcggca cagaaacacc agaaatgcgc
    24001 aaccgaaaac tggcacatca cggtggcatc acatttgaga ctcaaacagc tccaggtgca
    24061 gagcgcttct cagcatttgg ttcgattcat ttaaaagccg gatcagtatt tgaaacagtg
    24121 acagaattca aaatcaaaac aagaaaggaa taactcatca tggaaatcaa aagaagctta
    24181 aacacaaaat ttgccgaaac attcggacct aaaaaaacta cacaatactt ctcaccagga
    24241 cgcatcaatt tgattggtga acacacagat tacaatggag gccatgtctt ccccgcttcc
    24301 atcacttatg gaacgcaagg tgtagcagca cctcgagacg ataataaaat attagtttat
    24361 tccacaaact ttgacgatat cggtgtcatt agttttactt tagatgagct agaatatgat
    24421 aaaaaagcaa actgggcaaa ttacgtaaaa ggaatggtcc ttaaattaaa agaagctgga
    24481 cactcaatcg atcatgggtt tgagttgtta gtggaaggaa caatccctaa tggtgcagga
    24541 ctttccagta gcgcttcatt agaattatta gtaggtgttg tattagaaga tctgtttgat
    24601 ttaaaaacag atcgcttgga actagtccaa acaggtaaaa aagtagaaaa tgaattcatc
    24661 ggtgtcaatt caggtatcat ggatcagttt gccatcggct tcggtgagct agatcatgca
    24721 atcttattag atacaaatac gctgaaatac gaaatggttc cagtgaaact agatggttat
    24781 gcaatcgtca tcatgaatac gaataagcgg cgggaattgg cagattcaaa atacaatgaa
    24841 cgccgtagcg aatgcgaaga agccttggca cgtttgcaaa aaactttgcc tatctcttct
    24901 ttaggagact tagatgaaga agaatttgaa tcaactattg accaaatcgg cgatgataca
    24961 ttgatacgca gagctcgcca tgccgtttct gaaaatcagc ggactttgaa ggcaaaagct
    25021 gaattagaag ctgggaactt agaagcattt ggtcaactgt taaatgattc tcaccactct
    25081 cttcgctatg attatgaagt aactgggatc gaattagata cactagtaga tgctgctcaa
    25141 aaacaagaag gtgtcttagg tgcacgaatg acaggagctg gatttggtgg ctgtgcgatt
    25201 gctcttgtca aagaagaaaa tatacctgcc ttcaaaaaca atgtctatga tgaatacatg
    25261 aaagttgtcg gctatgctcc ggaattttat gttgcacaca ttggaaatgg gacaacaaag
    25321 atcgatccag aagctgttca tgtttgataa aaaaatcaac gatctcagct aaaaacaaat
    25381 tgataaaaat aacctctcag caatttccaa atgtgagcct tttgctcgta tttgaaaatt
    25441 gctgtttttt attgacacgg aaagagaacg tgtgttctaa ttgtgttgaa gaggtgattg
    25501 ttatgtgggc gaatcaaaat ccggtatttg tttatgaaaa agaaccttca agagatattt
    25561 tatgtattga ctgcaaatct ttttacgcct ccgtagagtg cgttgaaaag gatcttgatc
    25621 cccttacaac taaattagta gtcatgtcct atccgtccga ttcgacagaa acacggggta
    25681 gcggattaat cttggcttcc agtcctgcag caaaaaaagc ttacggcatc accaatatca
    25741 gccgtgccag agatcttccc ttcccctatc caagtgattt atatattgtt gccccaagga
    25801 tggcctatta tatggaaaag aacagagaaa tcaattctat ctataaaaaa tatgcagacg
    25861 aagcgaacca tcatgtctat tccgtcgatg aaagcttcct tgatgtaaca aatagcatgg
    25921 cactctttgg taaaacaaat gcgtacgaca tggctaaact gatccataaa gatgtgtttg
    25981 agcagaccgg aatctacacg actataggaa ttggcgataa cccgctactt gctaaactag
    26041 ccttagacaa tggctcaaaa gacggaaagc aaatgtttgc tgaatggcgt tatgaagatg
    26101 taccagaaaa gttatggaaa atctcagata tcactgactt ttggggaatc ggcaaacgaa
    26161 cagcaaaacg tctgaaacaa atgggtatac attctgttta tgatttagct cacgcggatt
    26221 attatcactt gaaaagcaag ctaggggtca tcggtactca gctttatgcc catgcttggg
    26281 gcatcgatcg aagttttttg ggacaagaat atacaccaaa atcaaaaagt atcggaaata
    26341 gtcaaatcct ggatagagat tatacaagac gagaagaaat cgagatcgtt atcaaagaaa
    26401 tggccgacca agtcggcact cgcttgagac gatctgcagc aaaaacacaa gtagtcagct
    26461 tatgggtagg atactcttta ggctatcttg atccaaatgg caagaccggg tttcaccagc
    26521 aaatgaaaat cgaagcgaca aatgctagca gagatctagc agatgcttta ttgaccattt
    26581 tccaccagca ttaccactac caggatattc ggcgtgtagg cgtgaactgt tccgacctcg
    26641 tatacgcgac aggtttacag cttaaccttt ttgaagatcc tgaaaagcag ataaatgaag
    26701 cgaaagttga ttttgttgtc gatacgatta gaaaaaaata cggctttcaa tcaatcatcc
    26761 atgcgcatag tctcttgcaa ggaggacgcg ccatcgcacg tagttctctc gttggcgggc
    26821 atgcaggtgg tatgagtgga ttagaaggtg gaaaagatga gacggacagc aaaaaaattc
    26881 attgactata acgagtatcg agatcggcca tttggattaa aatgggggac tgcttttgcg
    26941 atggaagaac tagtcaacgg tatcaaaaaa aatgaagaag aagcacgcaa acagcctctt
    27001 cctaaacaac aaatgacatt gcaagaaatc gatagtatac tgacagaagc ctttttatct
    27061 cgtaaagcag taatcattca gctcaattta aaagatgaat ttggaagact cttggatgag
    27121 gtcgaagggc acttcattgg tgaagcctat gaagattact ttatgattga ccatcagccg
    27181 atattgtggg aagacgtacg gtatattgag ttaaaggaaa caaaaaaatg gtttgtgaca
    27241 tgagaggttg tgacagaagt gatcaacttc aaaaaataag aaaggatttt tcgaaaattg
    27301 cttcttaaat ttttgtggaa ttttggctta tttttcgaag aagcaacttc tggaacaacg
    27361 tatattagat atcagaggtt gtgaaaaagt cgcttagttt tgagtaacaa gccaaggcgc
    27421 tgatttcaca acctctgtct atctatccat ctattcggta agattcatgc atgacgtctt
    27481 ctttatttag tatgtagagc cctttttctt ctgaccattc aacataaatc agctgtttta
    27541 catctgcata gccttctttc ttcaagttct ccataagcca tgcttcagtc aattgattgg
    27601 actgaagcac tttatgctga atttgccctt catctacaag caggatcgat aaagctttgt
    27661 cttcatctga ttttttcaat acactcattt gaccggcatt ctctaggata acatgtttgg
    27721 cattttcaag agaaaagcat tcttgctgtc ttaagattgc tcgcaactgc tccatctcga
    27781 tatggttatt tgtcaactct gtcatgttga tgacgccgtc tttgattaga acagatggct
    27841 cgcctttgac ccaacgattg accttttcat ttttttggac gatacgttcg ataccataaa
    27901 tcattactgc ccaaagcgca atggcaaata ggacatgccc tacatggaca ttgtcatcat
    27961 aggttgattc ttctaagatc ccacctaaaa caagcgtata gactaaatca aacggtgtga
    28021 tttctgacat ggatttcttc ccaagcaaac gtaccactaa taataaaccg atcaacccga
    28081 acaccagttt cattgtaatt gaaaaaatca ttttttctcc ccctaactat ctgttaatta
    28141 aattatatat gctttctcac gaacacgcat ggaatataca aggaagaaat gtatcctttt
    28201 actaaaaaaa ggactgtgac actgtagtga cccccaaaag ttagactttt ttggagtgga
    28261 gaaatccact cctttttttt atgctctaaa atgatatttt tacacaaccg ttacggttgt
    28321 tttacgaaat tggacgagac tttgccagtt taatttctgt tttatccgtt cattattgta
    28381 gtaatagata tagctatcga tggctgattt taattcctcg aaacagttat aaactttccc
    28441 gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat tatctaagca
    28501 gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt ttctataagc
    28561 tttcatttga tacgcccaac cttgatctga gtgaaacgta cgacgatagg gacaatcgtt
    28621 agtggtctgg atagcttcct ccaaaccttc cattaccgct aatgcattgg gcttttcaga
    28681 gatacgatag gataaaatct cactattgaa catatccata aaaggatcta gatacaattt
    28741 cttttgacgg ataatgcctt tggcatccac ttcatggtat ttaaattcag ttgtatccgt
    28801 cgtgattttc tgatggcaga tactggtgta gaaacgacga tggatccgat ttttggctac
    28861 tgctccaact tttcctcgat aagaattgta gcgacgtgat ttacgcgtgt atgccgttac
    28921 ttttattcct agcttcttga tcaagcgctg aactttcttt ttattgatag agaaaccgcg
    28981 gtttcttagt tcattggtca tccgtaaaca gccataatcc ttgtgcttct cccgaatttt
    29041 aagcatttcc gcttcaatct cttgatctgg atttttacgg tccaatcgcc tttgccatcc
    29101 aaataatcat gaacgatctt taatttaagt tcaaaagtat atttaaccat aaaaaatacc
    29161 gacctccaaa agttagattt tttggtctaa cttttggggg tcggtacaca aatcgtgtca
    29221 cagtcccttt tttgaaaata aacggtgttc aaaagcttgc atctgcggta ataagtccag
    29281 ccaagttaaa aacataagaa gctgttttta cttggctgtt cttattactt gatgcaaggc
    29341 agctttttca caacctcttt tcaaatattt cgttttctta ttctacaaat ggttcttctc
    29401 ctaacgcttg ttgagcggct agattcaaca tgctccattg tcggtcaaag cctggctgga
    29461 agaagaagtc agcttctgct agttcttcta aagtcagttt gttttggatc gctagtgcca
    29521 atacatttcc ttgacctgta acatcatagg tagataatac tgctcctcct agcaattgat
    29581 gggtcattgg atccgagaat aattggatgg acacttttgg atttccttgt tcttctggta
    29641 catattttgg tcgtaacgta tcttcataat aagacactcg tacatctact cctgcttttg
    29701 ctgctgtaaa gctattcaat ccgcttgctg caaaatgata gtcaaagaca cttaatgcag
    29761 aggaacctac gactccgccg aacggtttgt ttggtacttc ttcaaaaaga tgttttacta
    29821 cataacgtgc ttctctacgt gcaacagtcg ctaaagcgat cggcattttt ttgcgtgctg
    29881 ggatagaata agcaagtgta gcatctccta ctgcatacac atctggaaga ttcgtttgca
    29941 agtattcatt tgtttggatc cagccacgtt catctaagtc gacgatccct tttaaccatt
    30001 ctgtatttgc cttcacaccg gcagcttgaa tgactagatc tgctgggatt tctttttcac
    30061 ttgttttgat tgcctcaact tttccgttac cagtaaaagc ttcgattttt gcatttgtca
    30121 aaacttcaat accgttttca tttaggtgtt gttctaaaat atctgtcatc tcagcatcta
    30181 aataagtgcc taaaggtcta tcgatcacat caagcaatac gacttctttc ccagcttttc
    30241 ggcttgcttc tgctgcttcg atcccaatgt aacccgcacc tacgacagcg atttttttaa
    30301 tagccggatc atttaatttt tctttgattt tggttgccca attgtatcca cgcatcaagt
    30361 agacattttc tagatctgct cctggtactg gcagattgtt tggtttgaca ccagaactca
    30421 aaatcaattt gtcataagga tattcttttg tttcgttatc tgatgcgcta gtaaccgtta
    30481 ctgttttttt atcggcatcg atcgttttta cttcgtgatt atttaaaata tgaacattgg
    30541 attgcggaaa gttttctggt ctgaaattac gtacatcatt tacatctgtt accttgtctt
    30601 ctaaatacag ctccatacca catgacatga aggaaatata gtctcctgct tcaaatagcg
    30661 tgatatctac attttcaccg tatcgtgtca atagttccaa gatcgattga tgccctccat
    30721 gagatgcacc tacaatgaca actttgattt tttccattga tgaaacctcc ttgaatattc
    30781 ttttaaaatc attctaaaca atttaaactt aacattacat tagttatgaa tcaaaagaaa
    30841 cgcgttcatt tgaactaaat ttaaaaaaca atcgtctcct tgcagaatat accgcaaaat
    30901 taacgattgt ttttttgata aaatctatga atcactgttc tgttaaaaag tctttgatcc
    30961 gtttgcaggc ctcatgtaat ttttccatgc tgctagcata agaaatccgg atataacctt
    31021 ctccttctgg gccaaatgca attcctggga tacacgcaac ttttgcctgt tccgctaatt
    31081 ctacacaaaa ctgccaagag tcagtttgaa tggcagctgg gagtttgcaa aacaagtaaa
    31141 atgcaccgtt tggctgggtc acttcaaatc ctaaaggcgc caattgctgg accagatagt
    31201 ctcttcgtgt ttgatattgc tctttcatat tctcgctatc tttcacccca tttattagcg
    31261 cttcagccgc agctttttgc gacatcgttg tagcggcagt caccagatat tgatggactt
    31321 taattagttc gtcaataagg gctttttgag caaaaataaa gcccactcgc caaccagtca
    31381 tcgcatgtga cttggataac ccgcttatca cgattgtttg ttctggaaga taagtagcta
    31441 tggacatatg cgttccacta taagtcaatt cagcatatac ttcatcactt ataacaaata
    31501 ccggatattt ttttaaaacc tcagcgattg ctttcaattg ttttgcagac aatgtcgtcc
    31561 cagtaggatt gttaggatag tttaaaatga ctgctttgac agctgatcca tattgttcaa
    31621 atgctcgttc aaattgttcc ggctgacaga caaaccctgt gtcagaagta tctatcggga
    31681 tcaattcagc accagctagt tccactaatg gctgatatcc tgaataagca ggcgctggaa
    31741 taagaacctt gtctccttct tccaaaacag ataacaacgc agaggcaatc gcttccgttg
    31801 caccaactgt cgtcaaaact tccgtttgcg gatcataagt taatccataa cgctgttgct
    31861 gaaatataca agccgcttcc cgcagttcct ctaatcctct cattccggta tagtgagaaa
    31921 agtcttcttc aatcgctgct attcctgctt gcttgacatg ttcaggagtt ggaaaatccg
    31981 gttctcccag ggtgagtttg atcacatctg gtatcgacga tatttgctga tcgaattgtc
    32041 gtatttttga tacttctatt ctagaaagcc taggactaaa tcgattggat aatttcatca
    32101 ttatttactc gctcctcttt tgaactgcta tcgattttta caagaagatt ttctgtaaaa
    32161 agaatcgttt tgtaccattc tacaccattt tctaaaacat ccatatcaaa atcgaacgtc
    32221 ggatcatgta aagcaggggt atcaccgaca cctaaaaaga aaaatacaca tggatattgt
    32281 tgcgtatata tgccaaaatc ttcggcttga agaactggag catctaattc atgtagtttg
    32341 tttgaatcta acagtgctgc atataagcct ctatggttga ttacagctgg atagccgtca
    32401 ttatagcgaa aagaaatagt agctgtagag tgaaactgtt ctgctaaatg gtctaatccc
    32461 ctcttcaaat aattttgcat atctgaactg taagaacgga tacttccgga tacaataact
    32521 tctccagcta aaacatttcg gatcgttcca ccttggattt ggccaaattt cattagatga
    32581 ttccactctg ccggtaattg ccggtcaaaa tcataaacat tcttcaaaaa tcgtacagct
    32641 gcctccagac tatcgattcc tttttcacta gaagcaatat gtgcagactg gcctttaatg
    32701 ataatgtctg tttcactgct ttgtgccatc aaaggtcctg gtcgagagaa aacttctccc
    32761 ttagctagtc caggccatag atgaaagcca aatattgctt gtacttcata tttatccaac
    32821 aatcctgact gaataacgct ttgtgcgcca ccggaaattt cttctgatgg ctgatagatc
    32881 agtactacat tataaataag atcctcaatg tgtttagtta taaagtcagt caaccctaaa
    32941 agcatcgcca tatgtccgtc atgaccacat gcatgcatat aaccttcatg cttagaagca
    33001 aaagagagat tcgtttcttc tttgactggc agtgcatcga tatctgttcg aaaagcaatc
    33061 gtttcttctt gctgattatc aaaataaagg agtagtcctg tttcgccgat ttcatggata
    33121 acggccccgc tgttttttac ttgttcatat aaatattttt ttgtcttgaa ttcatgaaac
    33181 ccgatttcag gaatttgatg gagtgccatt cgatattctt tcagcatccg tattcaccat
    33241 cccttcatag atacccttca ctactgtttc atattgatta ttcggtacac cgatgatcag
    33301 attcaactcc tgtgctcctt gtgataagat cgatgtgcga atttctaatt tgttcaatat
    33361 cgataacact ttcccagata atccaatcaa ttctttatgc ggacccccaa cgacagagat
    33421 caaagctaag tcttcgatca cgacgatttc ttccacgcca agtgtttctt ttaattcttt
    33481 cgtgatgaga aataacttat cggcaatcgc ttctgctgag acgatcactc cgatattgtc
    33541 gatccctgaa ggaatatgtt cgattgatat ctcatgttta gcaaaaatgc tcattgcttt
    33601 ccagataaac ccgatttcat cggacatatg acgtttgaat aaagtgatgg aaagaaaatc
    33661 cttgcgtcca gctattcctg tcagtccatt ttccttttct atttcgtgat tcgatatttt
    33721 tgtcccttga gcgaaaggat cattggtatt ttttatttgg atggggatat cagcctcttg
    33781 aactgggaaa acagctgctt catgaagaac gttagccccc atataagcca tctctctcag
    33841 ttctctaaaa gagatttcct cgatgatttt aggatgatcg atgattcgcg gatcagccat
    33901 catgattcct gaaacatccg tccaattctc ataactttcc gcttgcaaca tcttcgccag
    33961 tatcgcgccg gtaatgtcgc ttcccccacg gcccaataat ttttcttttc ctgcagggtt
    34021 cgccccgtaa aaccctggaa caaccatttt cggatatctt ttaaatgctt ttgtgatttc
    34081 ctgtgctgtt ttttcctgat cgattttccc atcatagtca aagaaaatca ggtcttttgc
    34141 gtcaataaac tggtaaccaa gatactcagc catcagttga gcagtcagat attcacctct
    34201 acttactaaa taatcttcag ttatcttttc gtcttccagt tgtttctgta tgcgtgctag
    34261 ttcatcttca ataggtaact gcagggcaag aacatctctt acttgaatga aacgatcaca
    34321 aatccttttc caaaggggtg tccaatcaac atgatgctga aggtaagcat aaatcaaata
    34381 aagtaagtct gttactttgt catcatctga cgtacgtttg cctaaagcag aaacaacgat
    34441 cacttggcga ttgggatctg aaagaacaat tgtacggact tttttgaatt gctcttcatg
    34501 agacatcgat gttccgccga atttcgctac tttcagcgta ttactcatct tgccacctcc
    34561 gctattttct gcattaaagc tgcagcaatc tgtacggcat tcgcagcggc tcctttgcgt
    34621 atgttatcgg cagtcgtata gaacaacaat ccgttctcta aagaagtgtc ttttctgatc
    34681 ctcccaacat aaaccatatc tgttcctcta gcaagaatgc tcgtcggata tttatgtgct
    34741 gctagatcat cttctaattg aatccctgga aactggctga gacactctct aatttcttcc
    34801 gtcgtaaact ctttttgcag ttgaactgcc acagagactc catgtccatt ttcgattggc
    34861 acacgtacgc aggttgctga tactggcaaa gcaggctgat gcagaatctt acgggtctca
    34921 tttatcattt tctgttcttc ttttgtatac ccattttcca aaggctgatc gatttccgga
    34981 atcaccgttt ggctaatatt ataaggatac aaaactggtt cctcgtcatt ttgcgtacga
    35041 tgaagatctt caatgccttt ttgcccggaa ccagaaactg cttgataggt cgaataattg
    35101 actcgctgga taccaaaggc ttcttgtaaa gctttaagcg gaagtaccgc ttgaatggtt
    35161 gaacaatttg gatttgcaat cagccgattc aatgtatagt cttccagatt gacctctgga
    35221 acgattaatg gaatatccga atcttcccgc catgctgagg aattatctat gacaaatagt
    35281 ccctgacttc gtgcgattgg cgcaaactct ttggatacag aagcacctgc agaaaatagc
    35341 gctaaatcat atccttcaaa tgaagtttct gtcaattcct gtacagtgtg tttctttttc
    35401 ttgaatacac aaactttacc ggctgaacgt tttgaagcta ataatttcaa tcgtcctact
    35461 ggaaaatcat attcttctaa cactttgagc atcgtttggc cgactaatcc ggttgccccc
    35521 acaatacagg tatctatttc gcgcattttt ctccatctcc tctataattc gcgtaacgca
    35581 gcaatcaatg ctgtattttc ttttgtctga tcatcactta tttttaatac acgtgcaggt
    35641 attccaccga caacagtttc tgctgccaca tcttctaaaa ccactgctcc tgctgccacg
    35701 actgcatctt ttccgatatg gacaccttca acaatcactg cattcgcacc taccagtacg
    35761 ccgtcttcta cgataaccgg tttggccgat gctggttcga tcacacctgc taagacagct
    35821 cctgcaccaa tatggcagtt tttgcccact gtcgctcgtc cacctaagac agcacccata
    35881 tcaatcatcg tgttttcacc gattactgca ccaatattga taatcgctcc catcatgatc
    35941 acggcattgt tgccgattga cacttgatct ctgataatag cacctggttc gatacgagca
    36001 ttaatttttt taatatctag caaaggaact gcgctgtatc ttgcagcagt ttcgatttcg
    36061 atttcaatga ttttttctga ttcagcttct aaaaccggag caatcgtctc atagtcacca
    36121 aaaacaacga agctgcttcc ttcaccaaaa actttacttt ctttaaattc gattggctcg
    36181 gttacattca gatagacttt cacaggcgtc tttttagatg ccgtttgaat gtagcggatg
    36241 atttcttgag cttccacgtt ttttacaccc ctttgtccat taataaatct tctaatttgt
    36301 aataacctgg tgactgatct ttcaaccagt ccgctgcttc tactgcgcct gcagcaaaga
    36361 ttcgattaga aaaagattca tgtttgatcg taaatatttc atcagtagca gcaaacaata
    36421 catcatgttc accaggtaaa gaacctgcac gaatactgtg tacaccaatt tggttttctt
    36481 ctcttttttt cccttgccag ttgtatacgg gctgcagctt ttgcacttct tggattgttt
    36541 gaagcagcat atttgcagta ccagatggtg catctttttt acgactatga tgtttttcaa
    36601 tcagttcgat ctgccagcct tgcaacattg cagctgcttg cttgattagt tgattcatca
    36661 ccatgatgcc gaacgaaaaa ttagcactat acatcacagg tacatgtttg gcatgttctt
    36721 ttatttcttg gaactgtgat tgtgtataac ctgtcgtgcc aatgactaac ggaagttgat
    36781 atgataagct atacgaaagg attttctcta aattatctgg atgagaaaaa tcaataagca
    36841 gttctggcag ttccgtaaaa gcaagcgtag ttgcttcttt aggtgccgtc cgtaaaagaa
    36901 ggaccgtatc tcctctttct tgtgcaactt cttcgactct atttcccatc tgtccactgc
    36961 caatcagtcc gattttcatt tttcaaccgt cctttctttt aacaaaagct gagctaattg
    37021 tttctttttc tcttttgaaa gagaaactaa aggcaggcgt agaggtccgg aaggtaagtc
    37081 taataatgac atggctgctt tgacaggaat aggattggtc tcactgaaca aggcatggat
    37141 cagttcaagt gcatttaatt gcttttcttt tgcttgctgc catcttccgt cctgcgtatc
    37201 ttttactaat tcatggacta ttttaggttg gatattcgcc catacactta ttacaccgct
    37261 tcctcctaca ctcattactg gtaggatcaa atcatcattt ccactgtaaa ggaagaattc
    37321 ttctccaatc aaccgagcgg catccatcac ataagccata tctcctgagg cttctttcaa
    37381 accaatgata ttaggatgtt ctgctaaatt tactaataca tggattggaa tcgacatgcc
    37441 tgttctagaa ggaacattat acaaaataag cggaatcggt gaggcttctg caattgctgt
    37501 aaaatgagca gctagtcctg catcacttgt tttgttgtag taaggggtga tgaccaaaag
    37561 tgcgtctgcc cccaaagaag caaaatgctt cgctttttca atcgtttctt tcgtattatt
    37621 cgttccagct cctgcaatca ccggcacacg gccagccact ttctttactg tcagttgaag
    37681 gatctgctcc tcctcagatt gtgtgagcgt agaagattct cctgttgtac ctaaaactaa
    37741 taatccatct gtttgttcat ctaaatggaa ttggatcaat ttttccaaac cagcataatc
    37801 tactgatccc tcttcattca tcggcgtgat cagcgccacg atcgaacctt ttatcatctt
    37861 cattcctcca atattcttgt ccattcatga actgctgggc cagccatttg tatcgcatcc
    37921 tcaccagaga tcatcaagtc tccttgttcc aagtgaacaa ctacctgatc tgctgaaatt
    37981 ttcccagttt ccttagcaac cacataagag gcacagcaac ctgtaccgca agcaagtgtc
    38041 caacctactc ctctttcata ggtacggacg atcaattctt gttcattgac tacttctaca
    38101 aagttgacat tggttttttc tttgaacaaa ggatgatgac agatcagttc acctcttttt
    38161 ctccagactt cctcacgcgc attttcaaca aaaacaacgg tatgaatcgt tcctaaaaat
    38221 aaactagtga tcgttacttt ttcaccattg atctctattt cctggttttt tattggataa
    38281 gtttgattag ctttgatttt ttccggagaa tcatctggca ctcccatatc tacgctgcaa
    38341 taaaaaggtg agggatccaa caccgtaatt tttcttgtgc ctgctaatgt ctctacgttg
    38401 aattcctgtt cattcatcat gttctgatca tttacgtatc gagcaaaaca gcgaatgcca
    38461 tttccgcaca tcggcgcgcg gctgccatct ttgttgtaat agatcatttc caacggatcg
    38521 atttttacgg cgatcaatcc gtctgtatca tatttttctt ttgtgcataa agaagcggct
    38581 aattgactat aatctgtgtc ttctgcgtaa tctaataaga taaaatcatt tccgcagcca
    38641 ttatatttat gaaggatcat aggaaacttc cccccttagt agatcagaaa atgcttgtct
    38701 gcgaacaatc agtcttgatt ggccattatt gacaaaaaca actgctggtc gtgtcgctcg
    38761 attgtagttg ctgctcatcg agtatccata agctcccgtg gaataaacag ctaaaagatc
    38821 acccgattct gctttcggca aataagcttc tttaattacc acatcgcctg attcacacat
    38881 ttttcctgca atcgtcactt tctctgtttt ttcttccgac agttttgctg ctaaatcaca
    38941 atcgtattct gcctgataca gagctggacg gatattatct gtcatcccgc catctacaaa
    39001 gtaatatttt ttatgtggtg tctttttgat gaaaccaatc gtgtatagtg ttgtaccagc
    39061 ttctccaaca atacttctac ccggttcgat cagtaattga cgaatggtca gttttcgttt
    39121 ttccaaagct tcttccgtat attcaatcag tttttttagt gcctcttcga ctgggatcgg
    39181 ctgatcattt tgtgtataac gaatcccaaa ccctccgcca agattcaagg aaagaggttg
    39241 gtcaaaatcg gttaagtaat tcgtcaattt gtcgatttca gctaaccaag cagtaacatc
    39301 aaaaatttgt gacccgatat gagcatgaaa tccttctaac tctagatagc tgctttcttt
    39361 tatcaactga agggcttgtt ggcactcttt actttcatag ctcatgccga attttgaatc
    39421 aatatgcgca gtgacgatat attcatgagt atgtgcttct actccgacat tcaaccgaag
    39481 catgatacag atttttttta aatagcgttg gcttaattct gctaataatt caacttccat
    39541 caaattatcg gcaacaatat gcaacaaatc attttcaagt gcatagatca gttcctctgg
    39601 cgtcttgttg ttaccatgga agtagatagt atctctagga aaatcagctt gcaaagcggt
    39661 atagatttcc ccaccactga caacatctag cccgaatcca tattcacgaa ctaaatgcag
    39721 catctcaatc gtttggaatg ctttagaggc gtaaagcact tttgtatcaa aacgtttcga
    39781 tacaaagcct cgcttgaatg acagcatcgt ttgtctcatc ttttcttcat catacacata
    39841 cagaggtgtc ttatactttt ctgccaatgt gcttgctgtg actccgccaa ttatcaattc
    39901 tgtcattttt tcattcctct tttcactttt tttccgtttt tattggaatt tcatttgata
    39961 aagatttgaa ttttattgga ataatattaa acaaaatttt ctgaattgtc tagtatattt
    40021 cgaaatattt ttttaaaaat aagaataaaa atggttataa aaacaaaata cccgaactat
    40081 tagaagctat ttatacataa aaacataaat tcaacactat ttagtgatga aaaaatattt
    40141 tcgcttcaat atagttcgga atagttcgga tattttcaat tttatgaatc atttcaattt
    40201 cttcataaat aagtgcagtt tcttctttct ctagcttttg ccattcatct ttcaaaatat
    40261 aaataaaatc aaatactttt ttatttttca atttgctgat tccacaaaaa acctcttgaa
    40321 aggcagatcc gatgatctgc ctttcaagag gtcaagctct gttttcaaat tcttatcaat
    40381 ccgttagcga attattttga cgcacgtttt acataagaac cttctgctgt attgatttcc
    40441 aacaattcat ctgtttcgat gaagtcagga acattgacta ctaagccagt ttccattgtc
    40501 gctggcttac ctgaaccagt gactgttgca cctttgatgg aaggttgtgt ttccacaaca
    40561 cgtaatacga cagttgttgg taattgaacg ccgatgactt cgctaccgaa aaattggatt
    40621 tttacttcca tgttttctag gatatatttc atttcttctt ctactgtttc tacaggaatt
    40681 tcatattgct catatgtttc caaatccatg aagaaagctg tatcatccat cgtgtacaaa
    40741 tattgtacag gttttgtgtc gatatgtgct ttttcaaatt tttcttctgg tctgaacgtt
    40801 gtgtcgtatg ttgcacctga acggacatct ttcagtttca tacgcataac tgtatttcct
    40861 ttaccaggtt tatggtggct tgcatccaga actttaatca gtttgccgtc ttgtacgaaa
    40921 gtcataccgg ctcttaaatc acttgctgag atcattattt ttgctccttc ttttctttat
    40981 cataatcaac tttattgtat cagacaaccg gccagaaaac tagagtattc tctatctttt
    41041 aaggtctcat gcctatttgt cagaaaaata aacacgggta aacgatgcta tcgaaatatt
    41101 tttttctttc aagcctttga cttttatttt tttcatgcta tcgttaataa aaattttact
    41161 tggagggata cacatgacag tcagcaacca aaaacaattg aatcgttgct gttgtatggt
    41221 gcataaaagc agcatactat ttgtcatgtc tattaattgc ccttcataga atgagtacca
    41281 ggtaacttat ctttttatct actcaatttt tctctatgtc tttcaaggag ctgatttaat
    41341 agtcagctcc tttttttatt atcttttttt aggaggccca catgattatc tcgacaatta
    41401 cagaagctat tggttccaca ccgctttatc gctttgacaa tcagcactat tctatgccta
    41461 aaggttctgc tatttatgca aaactagaat atttaaatcc tggaggaagt atcaaagata
    41521 gattgggcag ttatttgatc aaagaaggat ttgcccaagg aaaaatcaat gaaaaaacga
    41581 cgatcatcga accaactgcc ggaaatacag gaattgggat cgctctggcc gcactcacat
    41641 acaaattaag aacggttttt gttgttccag agaagtttag cttagaaaaa caacaattga
    41701 tgaaagcatt gggagcaaag atcatccatt ctcctagtga gcaaggaatc atcggtgcca
    41761 tcagcaaaag taaaaaactt gcagaggaga tatcaaacag ctatctgcct cttcaatttg
    41821 aaaataaaga taatcctgct gcttactatc atacgttagg gccagaaatc tttcatgaaa
    41881 tcaaagaaaa tatccatagt tttgtagcag gaattgggag tggtggaacc tttgctggaa
    41941 cttctacctt tttgaaagaa aaatatcccg acattcgaat aatcggtgta gaaccagagg
    42001 gctctgtgtt aaatggtggt gatccagccc ctcacgaaat tgagggtatc ggtgtagaat
    42061 tcattccgcc gtttctttct cccctttcaa tcaatcagat cgagacaatc tctgatgtag
    42121 aagggtttaa ctatactagg cagttagcac gagaacaagg actattagtt ggaagttcga
    42181 gtggtgcggc ttttgcagca gcattaagag aaattcgtcg tttaccacct ggtcatcgtg
    42241 tcgttacgat ttttccagac gcagcagacc gctatttatc aaaaaacatt tactt
//
LOCUS       DACCPY010000009        42165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCPY010000009 DACCPY010000000
VERSION     DACCPY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.009"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            109..1173
                     /locus_tag="IVO69_000450"
     CDS             109..1173
                     /locus_tag="IVO69_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7399255.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1419..1844
                     /locus_tag="IVO69_000451"
     CDS             1419..1844
                     /locus_tag="IVO69_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7399256.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1837..2286
                     /gene="nusB"
                     /locus_tag="IVO69_000452"
     CDS             1837..2286
                     /gene="nusB"
                     /locus_tag="IVO69_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7399257.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2383..3237
                     /locus_tag="IVO69_000453"
     CDS             2383..3237
                     /locus_tag="IVO69_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7399258.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3238..4575
                     /locus_tag="IVO69_000454"
     CDS             3238..4575
                     /locus_tag="IVO69_000454"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7399259.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4580..4804
                     /locus_tag="IVO69_000455"
     CDS             4580..4804
                     /locus_tag="IVO69_000455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7399260.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4806..5696
                     /locus_tag="IVO69_000456"
     CDS             4806..5696
                     /locus_tag="IVO69_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7399261.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5705..6523
                     /locus_tag="IVO69_000457"
     CDS             5705..6523
                     /locus_tag="IVO69_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7399262.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6630..7079
                     /locus_tag="IVO69_000458"
     CDS             6630..7079
                     /locus_tag="IVO69_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7399263.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7097..8779
                     /gene="recN"
                     /locus_tag="IVO69_000459"
     CDS             7097..8779
                     /gene="recN"
                     /locus_tag="IVO69_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7399264.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8834..8950)
                     /locus_tag="IVO69_000460"
     CDS             complement(8834..8950)
                     /locus_tag="IVO69_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7399265.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9023..9979)
                     /locus_tag="IVO69_000461"
     CDS             complement(9023..9979)
                     /locus_tag="IVO69_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7399266.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10150..10518
                     /locus_tag="IVO69_000462"
     CDS             10150..10518
                     /locus_tag="IVO69_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7399267.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10688..11119
                     /gene="mraZ"
                     /locus_tag="IVO69_000463"
     CDS             10688..11119
                     /gene="mraZ"
                     /locus_tag="IVO69_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7399268.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11140..12099
                     /gene="rsmH"
                     /locus_tag="IVO69_000464"
     CDS             11140..12099
                     /gene="rsmH"
                     /locus_tag="IVO69_000464"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7399269.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12104..12505
                     /gene="ftsL"
                     /locus_tag="IVO69_000465"
     CDS             12104..12505
                     /gene="ftsL"
                     /locus_tag="IVO69_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7399270.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12506..14698
                     /locus_tag="IVO69_000466"
     CDS             12506..14698
                     /locus_tag="IVO69_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7399271.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14724..15686
                     /locus_tag="IVO69_000467"
     CDS             14724..15686
                     /locus_tag="IVO69_000467"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7399272.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15700..17061
                     /locus_tag="IVO69_000468"
     CDS             15700..17061
                     /locus_tag="IVO69_000468"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7399273.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17087..18175
                     /gene="murG"
                     /locus_tag="IVO69_000469"
     CDS             17087..18175
                     /gene="murG"
                     /locus_tag="IVO69_000469"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7399274.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18190..19410
                     /locus_tag="IVO69_000470"
     CDS             18190..19410
                     /locus_tag="IVO69_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7399275.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19566..20891
                     /gene="ftsA"
                     /locus_tag="IVO69_000471"
     CDS             19566..20891
                     /gene="ftsA"
                     /locus_tag="IVO69_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7399276.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20914..22155
                     /gene="ftsZ"
                     /locus_tag="IVO69_000472"
     CDS             20914..22155
                     /gene="ftsZ"
                     /locus_tag="IVO69_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7399277.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22172..22849
                     /locus_tag="IVO69_000473"
     CDS             22172..22849
                     /locus_tag="IVO69_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7399278.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22863..23462
                     /locus_tag="IVO69_000474"
     CDS             22863..23462
                     /locus_tag="IVO69_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7399279.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23477..23764
                     /locus_tag="IVO69_000475"
     CDS             23477..23764
                     /locus_tag="IVO69_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7399280.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23813..24595
                     /locus_tag="IVO69_000476"
     CDS             23813..24595
                     /locus_tag="IVO69_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7399281.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24616..25323
                     /locus_tag="IVO69_000477"
     CDS             24616..25323
                     /locus_tag="IVO69_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7399282.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25585..28383
                     /gene="ileS"
                     /locus_tag="IVO69_000478"
     CDS             25585..28383
                     /gene="ileS"
                     /locus_tag="IVO69_000478"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7399283.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28432..29958)
                     /locus_tag="IVO69_000479"
     CDS             complement(28432..29958)
                     /locus_tag="IVO69_000479"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7399284.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29973..30620)
                     /locus_tag="IVO69_000480"
     CDS             complement(29973..30620)
                     /locus_tag="IVO69_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7399285.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30804..31133)
                     /locus_tag="IVO69_000481"
     CDS             complement(30804..31133)
                     /locus_tag="IVO69_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7399286.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31310..32038
                     /gene="sfsA"
                     /locus_tag="IVO69_000482"
     CDS             31310..32038
                     /gene="sfsA"
                     /locus_tag="IVO69_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7399287.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32054..33067
                     /locus_tag="IVO69_000483"
     CDS             32054..33067
                     /locus_tag="IVO69_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7399288.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33067..34344
                     /locus_tag="IVO69_000484"
     CDS             33067..34344
                     /locus_tag="IVO69_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7399289.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34407..37109
                     /locus_tag="IVO69_000485"
     CDS             34407..37109
                     /locus_tag="IVO69_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7399290.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37261..37578
                     /locus_tag="IVO69_000486"
     CDS             37261..37578
                     /locus_tag="IVO69_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7399291.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37608..37928
                     /locus_tag="IVO69_000487"
     CDS             37608..37928
                     /locus_tag="IVO69_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7399292.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38036..39496
                     /locus_tag="IVO69_000488"
     CDS             38036..39496
                     /locus_tag="IVO69_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7399293.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39564..39785
                     /locus_tag="IVO69_000489"
     CDS             39564..39785
                     /locus_tag="IVO69_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399294.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39816..39998
                     /locus_tag="IVO69_000490"
     CDS             39816..39998
                     /locus_tag="IVO69_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7399295.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39998..40411
                     /locus_tag="IVO69_000491"
     CDS             39998..40411
                     /locus_tag="IVO69_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7399296.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40534..41715
                     /locus_tag="IVO69_000492"
     CDS             40534..41715
                     /locus_tag="IVO69_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7399297.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        14102 a         7158 c         9075 g        11830 t
ORIGIN      
        1 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
       61 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
      121 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
      181 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
      241 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
      301 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
      361 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
      421 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
      481 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
      541 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
      601 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
      661 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
      721 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
      781 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
      841 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
      901 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
      961 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
     1021 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
     1081 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
     1141 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
     1201 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
     1261 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
     1321 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
     1381 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
     1441 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
     1501 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
     1561 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
     1621 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
     1681 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
     1741 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
     1801 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
     1861 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
     1921 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
     1981 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
     2041 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
     2101 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
     2161 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
     2221 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg
     2281 gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc
     2341 tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac
     2401 ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa
     2461 gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa
     2521 atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag
     2581 cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa
     2641 gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa
     2701 aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta
     2761 ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa
     2821 atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc
     2881 aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt
     2941 gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta
     3001 gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata
     3061 gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa
     3121 gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc
     3181 acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg
     3241 gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc
     3301 gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc
     3361 aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc
     3421 aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt
     3481 gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag
     3541 cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag
     3601 gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc
     3661 cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat
     3721 ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat
     3781 gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt
     3841 cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg
     3901 atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc
     3961 gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt
     4021 cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg
     4081 tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta
     4141 tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag
     4201 cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta
     4261 agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact
     4321 gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag
     4381 cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga
     4441 ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa
     4501 ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata
     4561 gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga
     4621 aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt
     4681 taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac
     4741 cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag
     4801 ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct
     4861 tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag
     4921 tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg
     4981 atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt
     5041 attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac
     5101 ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta
     5161 caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc
     5221 ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata
     5281 tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga
     5341 cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag
     5401 aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag
     5461 atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg
     5521 aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt
     5581 tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt
     5641 cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga
     5701 aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag
     5761 agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt
     5821 tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact
     5881 tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc
     5941 tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc
     6001 tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg
     6061 gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa
     6121 acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc
     6181 actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc
     6241 tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg
     6301 tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg
     6361 atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt
     6421 cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc
     6481 agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat
     6541 caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta
     6601 agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta
     6661 ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa
     6721 gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga
     6781 tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag
     6841 atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg
     6901 aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg
     6961 ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga
     7021 ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag
     7081 ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga
     7141 attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa
     7201 atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat
     7261 acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg
     7321 attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg
     7381 acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt
     7441 agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca
     7501 ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt
     7561 ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag
     7621 ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt
     7681 ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga
     7741 agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg
     7801 agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact
     7861 gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc
     7921 ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt
     7981 agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg
     8041 taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct
     8101 agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa
     8161 agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa
     8221 agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt
     8281 tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat
     8341 cgaattttac attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc
     8401 tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg
     8461 tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc
     8521 catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct
     8581 gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg
     8641 acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg
     8701 tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa
     8761 actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag
     8821 ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta
     8881 aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt
     8941 tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt
     9001 ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta
     9061 cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa
     9121 taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa
     9181 tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa
     9241 aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct
     9301 ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt
     9361 tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa
     9421 cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa
     9481 gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga
     9541 gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg
     9601 gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa
     9661 gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc
     9721 taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg
     9781 attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat
     9841 ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta
     9901 cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta
     9961 atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata
    10021 gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa
    10081 taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag
    10141 tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc
    10201 gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata
    10261 aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat
    10321 acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt
    10381 attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg
    10441 acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac
    10501 atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt
    10561 gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga
    10621 aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg
    10681 gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt
    10741 gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg
    10801 ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat
    10861 gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga
    10921 atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc
    10981 tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac
    11041 acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat
    11101 gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac
    11161 tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat
    11221 gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct
    11281 aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt
    11341 ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta
    11401 gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt
    11461 tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg
    11521 gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc
    11581 taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt
    11641 gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc
    11701 gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa
    11761 agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct
    11821 ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat
    11881 tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta
    11941 ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt
    12001 aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag
    12061 ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa
    12121 aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc
    12181 tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca
    12241 gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg
    12301 tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat
    12361 acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga
    12421 actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga
    12481 cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga
    12541 aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac
    12601 gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca
    12661 tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt
    12721 aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc
    12781 ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta
    12841 tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga
    12901 caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga
    12961 atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat
    13021 gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa
    13081 tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt
    13141 agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa
    13201 gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga
    13261 aaaagctgtc gacggacaag atatctacac gactttagac agcagactac aaagttattt
    13321 agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct
    13381 gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga
    13441 aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag
    13501 ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga
    13561 attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa
    13621 tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag
    13681 taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca
    13741 aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt
    13801 acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac
    13861 gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc
    13921 tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga
    13981 agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga
    14041 tgttgtcgaa agcgaaaact acggtattgc ttatggtgta tatagtgtac ctggatacaa
    14101 tgtatcggca aaaaccggaa cagcccaaat cgcttctgat acagggggat atcaaacagg
    14161 agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt
    14221 cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc
    14281 aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga
    14341 aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc
    14401 agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca
    14461 aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac
    14521 tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct
    14581 ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga
    14641 acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga
    14701 agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca
    14761 gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc
    14821 aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta
    14881 caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt
    14941 ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag
    15001 taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact
    15061 cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt
    15121 ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt
    15181 atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg
    15241 gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg
    15301 cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac
    15361 tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat
    15421 tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc
    15481 ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct
    15541 ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt
    15601 gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt
    15661 tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat
    15721 gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta
    15781 ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg
    15841 gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa
    15901 ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct
    15961 ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac
    16021 gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca
    16081 acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt
    16141 aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg
    16201 atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg
    16261 attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa
    16321 gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat
    16381 cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca
    16441 aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa
    16501 aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta
    16561 gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg
    16621 cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa
    16681 ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt
    16741 gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa
    16801 ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag
    16861 ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa
    16921 caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca
    16981 gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa
    17041 gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt
    17101 tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa
    17161 gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa
    17221 aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa
    17281 aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa
    17341 aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg
    17401 agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag
    17461 tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt
    17521 taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc
    17581 acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga
    17641 aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt
    17701 atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga
    17761 tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca
    17821 gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc
    17881 tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag
    17941 cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc
    18001 agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga
    18061 aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat
    18121 ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg
    18181 gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa
    18241 aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa
    18301 aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa
    18361 gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt
    18421 tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg
    18481 ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc
    18541 actgtattgg gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta
    18601 caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca
    18661 aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat
    18721 cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat
    18781 gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa
    18841 tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa
    18901 aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa
    18961 tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga
    19021 gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat
    19081 atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa
    19141 aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa
    19201 acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca
    19261 aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca
    19321 aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt
    19381 acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact
    19441 ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta
    19501 tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc
    19561 cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag
    19621 ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat
    19681 cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac
    19741 gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt
    19801 tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt
    19861 caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa
    19921 tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg
    19981 agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt
    20041 ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa
    20101 tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag
    20161 aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta
    20221 tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta
    20281 aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact
    20341 acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg
    20401 gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa
    20461 tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc
    20521 caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac
    20581 aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc
    20641 cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc
    20701 aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac
    20761 aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc
    20821 cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata
    20881 tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc
    20941 aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac
    21001 cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa
    21061 gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt
    21121 ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc
    21181 ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga
    21241 accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact
    21301 gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca
    21361 gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt
    21421 ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat
    21481 gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac
    21541 cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc
    21601 ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca
    21661 ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg
    21721 gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc
    21781 gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg
    21841 acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct
    21901 agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa
    21961 ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat
    22021 gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca
    22081 ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc
    22141 cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa
    22201 gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt
    22261 agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat
    22321 gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt
    22381 tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt
    22441 aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa
    22501 gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa
    22561 acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa
    22621 aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca
    22681 taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca
    22741 tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga
    22801 aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa
    22861 aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag
    22921 agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa
    22981 atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa
    23041 aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag
    23101 taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa
    23161 cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt
    23221 attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt
    23281 ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt
    23341 atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg
    23401 ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt
    23461 aagaggagga actacgattt atactattgc actattactt catttacttc aacgggcagc
    23521 ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta
    23581 tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga
    23641 tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt
    23701 gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta
    23761 ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc
    23821 aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg
    23881 gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca
    23941 atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg
    24001 cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt
    24061 agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct
    24121 aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg
    24181 cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt
    24241 tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata
    24301 cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat
    24361 gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct
    24421 gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact
    24481 tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga
    24541 ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa
    24601 aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca
    24661 aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac
    24721 tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa
    24781 aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa
    24841 gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct
    24901 gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca
    24961 actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag
    25021 cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc
    25081 ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa
    25141 ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag
    25201 cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg
    25261 acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag
    25321 tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa
    25381 gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa
    25441 gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt
    25501 tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa
    25561 acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca
    25621 aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa
    25681 gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt
    25741 catgatggac ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc
    25801 agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca
    25861 ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt
    25921 aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta
    25981 gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga acatccttat
    26041 gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa
    26101 aaaagctata tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct
    26161 ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc
    26221 aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca
    26281 acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta
    26341 ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa
    26401 tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa
    26461 tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat
    26521 gttactttag acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac
    26581 tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg
    26641 tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc
    26701 actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat
    26761 cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca
    26821 tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc
    26881 ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc
    26941 cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc
    27001 atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc
    27061 tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct
    27121 aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct
    27181 catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa
    27241 ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat
    27301 ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt
    27361 aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt
    27421 gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct
    27481 atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc
    27541 ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta
    27601 cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa
    27661 gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg
    27721 gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa
    27781 gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca
    27841 aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa
    27901 gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa
    27961 gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg
    28021 gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat
    28081 ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg
    28141 atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct
    28201 atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc
    28261 tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt
    28321 tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag
    28381 taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt
    28441 taattttcca cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt
    28501 ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc
    28561 cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac
    28621 ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc
    28681 gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg
    28741 ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt
    28801 tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc
    28861 gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc
    28921 tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt
    28981 atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc
    29041 ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg
    29101 aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat
    29161 cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc
    29221 tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta
    29281 gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata
    29341 gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg
    29401 gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc
    29461 tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat
    29521 gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat
    29581 cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata
    29641 gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac
    29701 atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt
    29761 caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg
    29821 agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa
    29881 agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac
    29941 atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg
    30001 gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca
    30061 taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag
    30121 taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga
    30181 taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat
    30241 cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt
    30301 tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct
    30361 aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt
    30421 tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct
    30481 ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg
    30541 atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa
    30601 tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa
    30661 gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga
    30721 cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga
    30781 tatatatata cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca
    30841 gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca
    30901 cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg
    30961 atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag
    31021 aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc
    31081 aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt
    31141 atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt cttctttatt
    31201 tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt
    31261 tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca
    31321 gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa
    31381 aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct
    31441 gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta
    31501 tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa
    31561 tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc
    31621 atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga
    31681 gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc
    31741 gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac
    31801 gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct
    31861 tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc
    31921 gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac
    31981 tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa
    32041 agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt
    32101 tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt
    32161 ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt
    32221 gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct
    32281 tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc
    32341 agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt
    32401 gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta
    32461 cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg
    32521 ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg
    32581 tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc
    32641 aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg
    32701 gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta
    32761 tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat
    32821 tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc
    32881 tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa
    32941 agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca
    33001 agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga
    33061 tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat
    33121 aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa
    33181 caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct
    33241 ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa
    33301 gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa
    33361 gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa
    33421 ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata
    33481 aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg
    33541 aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt
    33601 ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt
    33661 gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta
    33721 cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt
    33781 catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg
    33841 tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca
    33901 gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct
    33961 agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa
    34021 gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt
    34081 acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata
    34141 acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa
    34201 gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac
    34261 ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga
    34321 aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca
    34381 aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat
    34441 tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc
    34501 gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg
    34561 ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg
    34621 tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag
    34681 tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta
    34741 tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa
    34801 tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg
    34861 ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag
    34921 gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag
    34981 atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag
    35041 gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg
    35101 gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg
    35161 caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc
    35221 tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga
    35281 ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga
    35341 aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca
    35401 ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac
    35461 cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat
    35521 ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg
    35581 aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca
    35641 tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga
    35701 ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg
    35761 ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc
    35821 tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata
    35881 acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg
    35941 aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt
    36001 ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga
    36061 tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa
    36121 aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag
    36181 tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag
    36241 atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga
    36301 aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg
    36361 gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg
    36421 atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat
    36481 gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc
    36541 aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca
    36601 tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt
    36661 atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt
    36721 taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat
    36781 cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc
    36841 agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc
    36901 atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac
    36961 tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg
    37021 cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa
    37081 tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac
    37141 atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt
    37201 ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa
    37261 atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc
    37321 acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct
    37381 gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct
    37441 caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta
    37501 gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca
    37561 gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac
    37621 aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa
    37681 tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg
    37741 atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag
    37801 cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga
    37861 catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca
    37921 gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta
    37981 ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa
    38041 cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca
    38101 aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg
    38161 atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac
    38221 ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg
    38281 aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc
    38341 ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg
    38401 tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt
    38461 gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc
    38521 agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa
    38581 cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat
    38641 gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt
    38701 tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt
    38761 aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc
    38821 attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt
    38881 attctggttc tttggtattc atggatcaaa tgtattggca ccagtgctag agggaatctg
    38941 gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg
    39001 tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc
    39061 gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat
    39121 cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg
    39181 tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat
    39241 gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta
    39301 tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt
    39361 gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt
    39421 cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc
    39481 aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa
    39541 tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa
    39601 tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac
    39661 aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag
    39721 caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg
    39781 tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat
    39841 gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt
    39901 tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg
    39961 ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa
    40021 tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa
    40081 agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt
    40141 ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa
    40201 gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga
    40261 cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa
    40321 gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa
    40381 aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat
    40441 tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc
    40501 ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt
    40561 gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca
    40621 cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt
    40681 gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac
    40741 ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga
    40801 caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta
    40861 agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa
    40921 tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa
    40981 tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat
    41041 gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat
    41101 aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca
    41161 acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt
    41221 ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt
    41281 gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat
    41341 ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg
    41401 tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa
    41461 gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat
    41521 catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc
    41581 acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag
    41641 gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt
    41701 aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt
    41761 ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt
    41821 ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa
    41881 tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct
    41941 tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg
    42001 ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct
    42061 tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta
    42121 ggatagagca atcgcctcct aagcgatagg tcgctggttc aattc
//
LOCUS       DACCPY010000010        41940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCPY010000010 DACCPY010000000
VERSION     DACCPY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.010"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..996
                     /locus_tag="IVO69_000493"
     CDS             133..996
                     /locus_tag="IVO69_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7399298.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1125..2747)
                     /locus_tag="IVO69_000494"
     CDS             complement(1125..2747)
                     /locus_tag="IVO69_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399299.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2996..3517
                     /locus_tag="IVO69_000495"
     CDS             2996..3517
                     /locus_tag="IVO69_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399300.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3703..5433)
                     /gene="spxB"
                     /locus_tag="IVO69_000496"
     CDS             complement(3703..5433)
                     /gene="spxB"
                     /locus_tag="IVO69_000496"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7399301.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5598..6416
                     /locus_tag="IVO69_000497"
     CDS             5598..6416
                     /locus_tag="IVO69_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7399302.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6519..6716)
                     /locus_tag="IVO69_000498"
     CDS             complement(6519..6716)
                     /locus_tag="IVO69_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399303.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6885..7463
                     /locus_tag="IVO69_000499"
     CDS             6885..7463
                     /locus_tag="IVO69_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7399304.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8506..9009)
                     /locus_tag="IVO69_000500"
     CDS             complement(8506..9009)
                     /locus_tag="IVO69_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7399305.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9368..10393
                     /locus_tag="IVO69_000501"
     CDS             9368..10393
                     /locus_tag="IVO69_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7399306.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10740..11618
                     /locus_tag="IVO69_000502"
     CDS             10740..11618
                     /locus_tag="IVO69_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399307.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12094..12210
                     /locus_tag="IVO69_000503"
     CDS             12094..12210
                     /locus_tag="IVO69_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7399308.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13138..13476
                     /locus_tag="IVO69_000504"
     CDS             13138..13476
                     /locus_tag="IVO69_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399309.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13570..13800
                     /locus_tag="IVO69_000505"
     CDS             13570..13800
                     /locus_tag="IVO69_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399310.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14200..14571
                     /locus_tag="IVO69_000506"
     CDS             14200..14571
                     /locus_tag="IVO69_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7399311.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14845..15537)
                     /locus_tag="IVO69_000507"
     CDS             complement(14845..15537)
                     /locus_tag="IVO69_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7399312.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15540..16325)
                     /locus_tag="IVO69_000508"
     CDS             complement(15540..16325)
                     /locus_tag="IVO69_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7399313.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16562..17962
                     /gene="lacG"
                     /locus_tag="IVO69_000509"
     CDS             16562..17962
                     /gene="lacG"
                     /locus_tag="IVO69_000509"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7399314.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17987..18304
                     /locus_tag="IVO69_000510"
     CDS             17987..18304
                     /locus_tag="IVO69_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7399315.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18317..19990
                     /locus_tag="IVO69_000511"
     CDS             18317..19990
                     /locus_tag="IVO69_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7399316.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20004..21635
                     /locus_tag="IVO69_000512"
     CDS             20004..21635
                     /locus_tag="IVO69_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7399317.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22507..23697
                     /locus_tag="IVO69_000513"
     CDS             22507..23697
                     /locus_tag="IVO69_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP7399318.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23831..26020
                     /locus_tag="IVO69_000514"
     CDS             23831..26020
                     /locus_tag="IVO69_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7399319.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26017..27810
                     /locus_tag="IVO69_000515"
     CDS             26017..27810
                     /locus_tag="IVO69_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7399320.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28010..28324
                     /locus_tag="IVO69_000516"
     CDS             28010..28324
                     /locus_tag="IVO69_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7399321.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28345..28722
                     /locus_tag="IVO69_000517"
     CDS             28345..28722
                     /locus_tag="IVO69_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7399322.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28732..29505
                     /locus_tag="IVO69_000518"
     CDS             28732..29505
                     /locus_tag="IVO69_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399323.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29527..30930
                     /locus_tag="IVO69_000519"
     CDS             29527..30930
                     /locus_tag="IVO69_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7399324.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31112..32296
                     /locus_tag="IVO69_000520"
     CDS             31112..32296
                     /locus_tag="IVO69_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7399325.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32293..32583
                     /locus_tag="IVO69_000521"
     CDS             32293..32583
                     /locus_tag="IVO69_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399326.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32580..32801
                     /locus_tag="IVO69_000522"
     CDS             32580..32801
                     /locus_tag="IVO69_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399327.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32843..33622
                     /locus_tag="IVO69_000523"
     CDS             32843..33622
                     /locus_tag="IVO69_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP7399328.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33689..34183
                     /locus_tag="IVO69_000524"
     CDS             33689..34183
                     /locus_tag="IVO69_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7399329.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34239..34877
                     /locus_tag="IVO69_000525"
     CDS             34239..34877
                     /locus_tag="IVO69_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399330.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34949..35344
                     /locus_tag="IVO69_000526"
     CDS             34949..35344
                     /locus_tag="IVO69_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7399331.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35328..37781
                     /locus_tag="IVO69_000527"
     CDS             35328..37781
                     /locus_tag="IVO69_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7399332.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37778..39805
                     /locus_tag="IVO69_000528"
     CDS             37778..39805
                     /locus_tag="IVO69_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399333.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39802..40824
                     /locus_tag="IVO69_000529"
     CDS             39802..40824
                     /locus_tag="IVO69_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7399334.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40841..41770
                     /locus_tag="IVO69_000530"
     CDS             40841..41770
                     /locus_tag="IVO69_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7399335.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT        13742 a         6942 c         8290 g        12966 t
ORIGIN      
        1 ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa
       61 agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag
      121 gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta
      181 ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta
      241 gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt
      301 gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat
      361 gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac
      421 agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat
      481 gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga
      541 ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg
      601 aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt
      661 tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat
      721 aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca
      781 atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct
      841 tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag
      901 atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc
      961 tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa
     1021 agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc
     1081 tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg
     1141 ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga
     1201 tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga
     1261 tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct
     1321 gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc
     1381 gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa aacattcact
     1441 ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg
     1501 ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta
     1561 tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt
     1621 actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt
     1681 atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca
     1741 tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt
     1801 ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata
     1861 tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat
     1921 gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg
     1981 gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa
     2041 tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg
     2101 ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat
     2161 tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga
     2221 acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt
     2281 aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca
     2341 gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct
     2401 gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc
     2461 agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca
     2521 tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa
     2581 cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca
     2641 tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat
     2701 agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac
     2761 ttttatcatt attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt
     2821 caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa
     2881 agcggagcga aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt
     2941 tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga
     3001 tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg tacttgtctc
     3061 attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat
     3121 ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac
     3181 aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt
     3241 tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt
     3301 tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg
     3361 atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt
     3421 gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt
     3481 atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag
     3541 ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa
     3601 atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat
     3661 atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata
     3721 taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc
     3781 ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt
     3841 attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa
     3901 tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt
     3961 accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc
     4021 tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata
     4081 tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt
     4141 aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc
     4201 catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg
     4261 aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat
     4321 acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa
     4381 agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt accagctatc
     4441 agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag
     4501 gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat
     4561 aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa
     4621 gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa
     4681 accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac
     4741 aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg
     4801 accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc
     4861 tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc
     4921 gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc
     4981 ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt
     5041 acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt
     5101 atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat
     5161 ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac
     5221 agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc
     5281 atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga
     5341 attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt
     5401 taccattgcg attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga
     5461 tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt
     5521 cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt
     5581 caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac
     5641 ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct
     5701 ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga
     5761 ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag
     5821 atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg
     5881 cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta
     5941 cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag
     6001 gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta
     6061 tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag
     6121 ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac
     6181 aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag
     6241 tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg
     6301 tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt
     6361 ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa
     6421 aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt
     6481 agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt
     6541 gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg
     6601 accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct
     6661 gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa
     6721 aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca
     6781 cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc
     6841 tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc
     6901 ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc
     6961 atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct
     7021 atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag
     7081 ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt
     7141 cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa
     7201 tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac
     7261 ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga
     7321 aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc
     7381 cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta
     7441 ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga
     7501 aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg
     7561 gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg
     7621 gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca
     7681 gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg
     7741 tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag
     7801 aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata
     7861 aaaataaaaa attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca
     7921 gaaatgaata aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa
     7981 aaaaataaca tatctcattt tacgcttttg ttattttata cttaaaaaat aacgacacag
     8041 aaaataccgt gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa
     8101 atgccttcaa aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct
     8161 aaataaggag aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata
     8221 gaaaaataat atacctgagt agggtataaa ttaagacgga attcctttct agattgagat
     8281 attgactagg aagaatgtta agattttaag cctttaagct aaaaaaaccc tttttgttaa
     8341 ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt
     8401 agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga
     8461 tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac
     8521 agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat
     8581 aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca
     8641 taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat
     8701 accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt
     8761 tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac
     8821 aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa
     8881 agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag
     8941 cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac
     9001 ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca
     9061 taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt
     9121 ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct
     9181 atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg
     9241 tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc
     9301 tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag
     9361 ggatagattg ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat
     9421 catgaatctg atgctactta tctttattgt attatggtta ggttacataa atcatgattt
     9481 tttgatttca agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc
     9541 gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt
     9601 tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc
     9661 tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt
     9721 tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt
     9781 ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt
     9841 actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga
     9901 agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta
     9961 tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat
    10021 tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc
    10081 gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac
    10141 catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc
    10201 agttgctaat atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc
    10261 atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg
    10321 tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca
    10381 aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca
    10441 caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa
    10501 aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttatatt
    10561 tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa
    10621 tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt
    10681 agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa
    10741 tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa
    10801 ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa
    10861 aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag
    10921 caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag
    10981 ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc
    11041 caagaactat ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc
    11101 aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct
    11161 tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg
    11221 atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat
    11281 ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc
    11341 ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta
    11401 aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag
    11461 ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac
    11521 atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg
    11581 agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt
    11641 aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    11701 ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    11761 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    11821 agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta
    11881 aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt
    11941 ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt
    12001 taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat
    12061 gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca
    12121 taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa
    12181 gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc
    12241 gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact
    12301 gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga
    12361 taccattccc taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat
    12421 aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt
    12481 attgatcact ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg
    12541 gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa
    12601 gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa
    12661 tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct
    12721 ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa
    12781 taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga
    12841 taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa
    12901 attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt
    12961 aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc
    13021 aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt
    13081 tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg
    13141 actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag
    13201 aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt
    13261 gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca
    13321 atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta
    13381 acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa
    13441 attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata
    13501 tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag
    13561 ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa
    13621 gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact
    13681 ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa
    13741 agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa
    13801 gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt
    13861 gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac
    13921 ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt
    13981 ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga
    14041 aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat agatttaata
    14101 agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa
    14161 ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat
    14221 aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa
    14281 gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa
    14341 atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt
    14401 gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc
    14461 cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat
    14521 aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta accattacgc
    14581 ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag
    14641 aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg
    14701 gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt
    14761 atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt
    14821 ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt
    14881 ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac
    14941 tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc
    15001 agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag
    15061 tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc
    15121 atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc
    15181 aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg
    15241 gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag
    15301 catacgataa tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg
    15361 ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat
    15421 atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc
    15481 atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt
    15541 tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca
    15601 tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc
    15661 aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat
    15721 ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta
    15781 gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt
    15841 cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg
    15901 ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta
    15961 tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta
    16021 cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt
    16081 tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa
    16141 gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat
    16201 tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta
    16261 acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc
    16321 aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc
    16381 taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt
    16441 gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc
    16501 gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta
    16561 catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga
    16621 aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca
    16681 agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga
    16741 attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt
    16801 tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga
    16861 agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa
    16921 acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta
    16981 tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat
    17041 ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga
    17101 ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa
    17161 ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa
    17221 atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc
    17281 gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc
    17341 tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt
    17401 tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca
    17461 ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg
    17521 gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg
    17581 aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa
    17641 aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga
    17701 tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt
    17761 ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc
    17821 gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt
    17881 tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag
    17941 tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga
    18001 attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt
    18061 gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga
    18121 agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc
    18181 atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac
    18241 cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg
    18301 ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt
    18361 tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat
    18421 gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg
    18481 ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg
    18541 tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg
    18601 tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag
    18661 tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac
    18721 aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg
    18781 tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac
    18841 cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg
    18901 cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct
    18961 atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt
    19021 ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta
    19081 tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact
    19141 gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt
    19201 tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc
    19261 ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt
    19321 gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa
    19381 gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc
    19441 agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc
    19501 ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt
    19561 cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc
    19621 cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga
    19681 aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt
    19741 aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc
    19801 tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta
    19861 ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg
    19921 agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca
    19981 atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat
    20041 ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg
    20101 aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat
    20161 attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact
    20221 atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag
    20281 ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta
    20341 aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc
    20401 ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa
    20461 agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca
    20521 atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag
    20581 aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac
    20641 ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt
    20701 ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt
    20761 atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac
    20821 ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg
    20881 atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac
    20941 cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata
    21001 atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg
    21061 caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca
    21121 accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag
    21181 caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca
    21241 gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg
    21301 aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg
    21361 catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg
    21421 taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg
    21481 aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc
    21541 caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga
    21601 cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat
    21661 cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc
    21721 ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc
    21781 agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat
    21841 gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta
    21901 gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac
    21961 agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata
    22021 gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa
    22081 gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt cccactctgt
    22141 tttcaacgta aagtgtataa aacagaatgc gatactatca agggagttaa aacaatatac
    22201 agaacagtaa aacactataa ataaaaataa aagtgcaata aaagtgcaat ttttgcacct
    22261 aatcatactc tgtttatata tgaaaactag attctgaatc caatatttgg ttaacgagag
    22321 gcgttcgaat ttgaacgtct tttatatatt actttcaaat aaatatgagg tagaatacta
    22381 tctattttct ttttaaccca attgttataa tggagagtgt aaaatatttt gtgtaaatag
    22441 aaaaaaggaa gtcccttctg tagaatagag ttaccacaac acattcacag aagagaggac
    22501 ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa aggcgatttg
    22561 aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa aacggagtta
    22621 acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg taattctcgt
    22681 aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct ccagattccg
    22741 cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg gacgaatgac
    22801 acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc ggaaatcgca
    22861 gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc caatataaca
    22921 aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga ccgttatgct
    22981 gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc caaggaagct
    23041 attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag ctatgcgatt
    23101 gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca agagcgcggt
    23161 ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg agcgattagt
    23221 cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg taatatcagt
    23281 cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat ggtgtatcaa
    23341 gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa atggaaaacc
    23401 agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac tttctatgat
    23461 ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc ctttaataaa
    23521 caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt
    23581 ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt
    23641 ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc aactgattga gaattagaaa
    23701 caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt ataatgagaa
    23761 cttttactaa cataatttat gataaactta ctatataatc atattaatta aatagttagg
    23821 agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt atgaatccga
    23881 aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt cagggaaaac
    23941 caatgctctt actgcattga gaatcttatt agatgatagt tattactata gtagtaaaac
    24001 actaaaagaa tcagattttt ttcatggaat tcaaaatggg tggcaagggc attggattat
    24061 tatctctgcg acatttgaag gaatcagcga agaagaattt gacaatgaga tttgtgcttc
    24121 catttcttta aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa atgcagataa
    24181 aggtgtaggt agtatttctc tatttattag acctaacaaa gctattagaa agcaattatt
    24241 tgatatttcc actgaattag actctcatca atttaacgaa tttagaaatt ctattcgtct
    24301 atccgactat gaattttact acacatctaa atcaaatcta aatttctgta tcaatgaaaa
    24361 ttatgataaa ttggtaggaa gactcaatga caatatagct agtaatcctg aagaagacga
    24421 tgaagctctg ttgggctcaa gaattgacat gtcagatatt ttcaagcata tttcggttgt
    24481 ttatgttgac gctcttcgag atgttcttag agaaatgaaa aacaatcgaa acccagtcaa
    24541 aagaatcatg gaaacaattg aatcaaaaat atcatcagat aatgttgagt cattaaaaac
    24601 aattatacag caattaaatg agactattac cagtgttcct gaaattagga aaattggcga
    24661 aaatattaat cggcaactaa atagcataat tggttcagtg tattctccta atcttcttct
    24721 ttcctcaact atgtcagatg atatgggttc tttagcaaaa tttctttcaa tgaagccaga
    24781 acaaaatatc gacttagact tactagggtt aggacatcta aatatgattt atttagcttt
    24841 gaaaatagtc gaatttgaag cgtgtcgttc aagagaacta cttaatatta tgttgattga
    24901 agaaccagaa gctcatattc atcatcatat tcaaaaaact ctgttcgaag gattaaatct
    24961 tcaaaaaaac tatactcaga ttctcatgac cacacattct gtacatttag ctgaagtgtc
    25021 tgaaatatct cggatgaatg tattaaaatc gtttaatggc aaatctatcg ttatgaatcc
    25081 agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc taaatctaat
    25141 aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt ctaaaggtgt
    25201 tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa aaaaattatt
    25261 tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta cttcgtttga
    25321 atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta ttgtaactga
    25381 tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg cacaagagaa
    25441 aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt gggttaattg
    25501 tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag aaaatattct
    25561 aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa taactgctta
    25621 taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt taaaactagc
    25681 caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg actttttaat
    25741 caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa tagtagatct
    25801 ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa ctacagaatt
    25861 gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata tagcaataat
    25921 ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca atcagataga
    25981 aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc
    26041 gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa cctttatgta
    26101 aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca
    26161 attaaacacg atggatcact aaaaagaata attgctatca catacactaa tagagctgct
    26221 gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa tatttgggtg
    26281 gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat gaatctctca
    26341 agagtatcaa aaggatttac aattattgat gattatactc aaagagctta cgtcagccaa
    26401 attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca
    26461 ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga aatgtatcac
    26521 actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt agcgtataaa
    26581 attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag ggcgatttat
    26641 gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa attacttcaa
    26701 aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat ttatggatca
    26761 ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca cacttttcaa
    26821 tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt ctactctaat
    26881 tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga ccgtggtttt
    26941 ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa aaatataata
    27001 aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc tcctcaacac
    27061 tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg caattttgat
    27121 gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa gttagcaatt
    27181 ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc aaacgatatt
    27241 ttaattatat taaaaaatga atttggaata gaagttaaag attattttat tgacttagat
    27301 ttattacagc taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat
    27361 ttatacaaat ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac
    27421 ccgtctattt ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat
    27481 aattcattat ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc
    27541 acacttacta cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat
    27601 cttctaaacg ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt
    27661 agttcagcga gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt
    27721 ttctcagagg tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta
    27781 gataaaatag aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata
    27841 gtaggaatcg gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg
    27901 aacttatgaa aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca
    27961 cacaaataag attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa
    28021 atatgtcatt cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa
    28081 cattgaacaa cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata
    28141 ctcaacgatt caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa
    28201 acattttgaa tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta
    28261 taaaattggc aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc
    28321 ataagaaagg aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac
    28381 cagacatttg gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa
    28441 gatggtacgg tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa
    28501 ggacgcatga tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac
    28561 gcagaagtgg aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg
    28621 gcagacgttg attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct
    28681 catcagaaat ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac
    28741 acaccattga ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac
    28801 cattattcaa agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa
    28861 aaattattag gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa
    28921 ctctttcccg acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt
    28981 cttccagaaa tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag
    29041 tggttgaaag aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga
    29101 aaatgtggct atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt
    29161 gtctatcgtg ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta
    29221 gaggaaaatg ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc
    29281 gaatacaaag ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca
    29341 aaagaagcga aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa
    29401 aaaaatttac gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca
    29461 acttttgata aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg
    29521 atttttatgc cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg
    29581 gtctatcatt ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt
    29641 cattggagag cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat
    29701 caacttcata cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt
    29761 atcagctggc tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa
    29821 aagttagctc gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag
    29881 ggattcttta aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa
    29941 atatattatc gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa
    30001 tatcaagatc agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta
    30061 gtggaaaaaa cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc
    30121 aatcgaattg gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg
    30181 gtctattggg agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga
    30241 aaatcctatt ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat
    30301 attcttgacc ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat
    30361 taccgtaaag aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc
    30421 agtgaaacca tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt
    30481 ttgtctgcca attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt
    30541 gaaagtacca ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc
    30601 ttctttttaa ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg
    30661 gacaacttta acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg
    30721 ttcggaagcg atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg
    30781 gatacaggaa aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat
    30841 ttcttaggag aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa
    30901 gtgaaaggta caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt
    30961 agccccctct atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac
    31021 caatgttgat ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag
    31081 tttacatctt ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga
    31141 attgaaagaa aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca
    31201 tattaccaga ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt
    31261 gaaagaagat ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt
    31321 attcgactat gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt
    31381 tttacgtttg aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga
    31441 acactatcgt ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt
    31501 attagaactg aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg
    31561 gagctggtat gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga
    31621 tttagctatc aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa
    31681 acaggaagaa tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt
    31741 tcaccgtgat gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga
    31801 agtctatttc tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt
    31861 agaagacgca gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca
    31921 agccatgaaa gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa
    31981 tcgctatatc cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa
    32041 tgaacgttgg gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc
    32101 agagccttat tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt
    32161 aaaaatcgca agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca
    32221 ggaagccaaa ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga
    32281 ggatttgatt acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc
    32341 attgagtttc aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga
    32401 aaacggcaag ctggtgatac catctatacc tatgattttt acaaggtgag attttataat
    32461 aattatacca atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg
    32521 cgtgtaaaaa gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca
    32581 tgaattttgg gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt
    32641 tagcgattgt tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg
    32701 gcttcttgat tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag
    32761 atgtgttatt aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa
    32821 aataggaagt atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt
    32881 taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg
    32941 taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt
    33001 tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag
    33061 agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc
    33121 aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact
    33181 tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca
    33241 agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag
    33301 agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt
    33361 tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga
    33421 aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat
    33481 gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat
    33541 tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta cagattatat
    33601 tagcggatac cttgataagt agcgtcatta actgattgct acttttttta tgactatttt
    33661 tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta
    33721 taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc
    33781 agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc
    33841 ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca
    33901 agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag
    33961 tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc
    34021 gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc
    34081 caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa
    34141 ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt
    34201 ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc
    34261 ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc
    34321 gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc
    34381 cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag
    34441 ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga gaatattttg
    34501 gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc
    34561 gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt
    34621 tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa
    34681 gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa
    34741 cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca
    34801 gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa
    34861 aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt
    34921 tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg
    34981 gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt
    35041 tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc
    35101 accactttct ctgattgacg gagcactact caaatactta gggattccca ttgttctcac
    35161 tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat
    35221 cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa
    35281 agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga
    35341 ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg
    35401 aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata
    35461 atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa
    35521 ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa
    35581 aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg
    35641 acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag
    35701 tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg
    35761 tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt
    35821 ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg
    35881 agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg
    35941 atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat
    36001 acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg
    36061 aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat
    36121 tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg
    36181 ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata
    36241 aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta
    36301 ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca
    36361 aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc
    36421 tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac
    36481 gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata
    36541 tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta
    36601 cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa
    36661 atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat
    36721 tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg
    36781 tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg
    36841 gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga
    36901 gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt
    36961 cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg
    37021 agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt
    37081 tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg
    37141 accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg
    37201 tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct
    37261 tacccgattc caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa
    37321 tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta
    37381 aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat
    37441 ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa
    37501 acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc
    37561 gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag
    37621 atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca
    37681 tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct
    37741 ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt
    37801 tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac
    37861 gctgggcaat gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga
    37921 atactccaag tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg
    37981 ctggcttcca tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc
    38041 cattactaat tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt
    38101 gaaagaagcc tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat
    38161 gcagacgatt gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt
    38221 cctccttctt tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg
    38281 agaaacgacg aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc
    38341 ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt
    38401 cagtaccgcc agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg
    38461 gaaagacagt gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg
    38521 gctattgctt caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag
    38581 tttattgtct accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga
    38641 agaaattgaa gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg
    38701 aacagtcttc tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac
    38761 aggaatcatg attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag
    38821 tttcctatta agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt
    38881 atttaatgtg attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat
    38941 ttccactatg ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca
    39001 aattgtgacc tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt
    39061 acaaagcaac gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat
    39121 gaatcgacaa accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg
    39181 gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac
    39241 tcgtcaaaat gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca
    39301 acaaggcggt atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa
    39361 tcgtctagtc aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt
    39421 caaatatgcc cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca
    39481 atctatggca gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa
    39541 acgaaagaac attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa
    39601 agaaaaccca tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac
    39661 cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa
    39721 agagcaaaaa caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac
    39781 ggataagcga ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc
    39841 tctctcttct tttttctctt attattccta gtcattttct ttgcagatga tacggacagt
    39901 ggcgaaaaca acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg
    39961 ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt
    40021 tcttatatct tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa
    40081 agttccgaat ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa
    40141 ggggttaaat atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat
    40201 tctgttatcc aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg
    40261 aaaaaatata cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa
    40321 gcagactatc ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga
    40381 aatcagtttt atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt
    40441 gatgatgaaa ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat
    40501 gtctttggag gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg
    40561 tatgacaaag ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa
    40621 gaaatctcta tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat
    40681 gcaggcacat atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca
    40741 ggcgacccaa tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg
    40801 gctagacgtg ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg
    40861 aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc
    40921 gatgattaaa gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg
    40981 tagtgtcgga tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga
    41041 aacagaaatc attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc
    41101 ctttgcgaaa gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga
    41161 aaaattgacc cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa
    41221 ggatattcct actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa
    41281 tgagaacacc tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa
    41341 ggaaactata tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt
    41401 tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca
    41461 gctagaaagt gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga
    41521 aacgttcttc cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa
    41581 tcatgtgtta cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt
    41641 taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac
    41701 aaaggcaacc caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat
    41761 tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg
    41821 ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat
    41881 ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc
//
LOCUS       DACCPY010000011        40814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCPY010000011 DACCPY010000000
VERSION     DACCPY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.011"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IVO69_000531"
     CDS             23..646
                     /locus_tag="IVO69_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399336.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVO69_000532"
     CDS             1096..2145
                     /locus_tag="IVO69_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7399337.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVO69_000533"
     CDS             2142..3023
                     /locus_tag="IVO69_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399338.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IVO69_000534"
     CDS             3026..3796
                     /locus_tag="IVO69_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399339.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IVO69_000535"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IVO69_000535"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7399340.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IVO69_000536"
     CDS             5107..6270
                     /locus_tag="IVO69_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7399341.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IVO69_000537"
     CDS             6387..6797
                     /locus_tag="IVO69_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7399342.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IVO69_000538"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IVO69_000538"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7399343.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IVO69_000539"
     CDS             9337..9654
                     /locus_tag="IVO69_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399344.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IVO69_000540"
     CDS             9814..10050
                     /locus_tag="IVO69_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7399345.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IVO69_000541"
     CDS             10047..10445
                     /locus_tag="IVO69_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7399346.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IVO69_000542"
     CDS             complement(11112..12056)
                     /locus_tag="IVO69_000542"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7399347.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IVO69_000543"
     CDS             complement(12352..13296)
                     /locus_tag="IVO69_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399348.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IVO69_000544"
     CDS             13517..14731
                     /locus_tag="IVO69_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7399349.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IVO69_000545"
     CDS             14753..16768
                     /locus_tag="IVO69_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7399350.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IVO69_000546"
     CDS             complement(16851..18224)
                     /locus_tag="IVO69_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7399351.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IVO69_000547"
     CDS             complement(18250..18534)
                     /locus_tag="IVO69_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7399352.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IVO69_000548"
     CDS             complement(18536..20551)
                     /locus_tag="IVO69_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7399353.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IVO69_000549"
     CDS             complement(20730..21035)
                     /locus_tag="IVO69_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7399354.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IVO69_000550"
     CDS             complement(21124..22155)
                     /locus_tag="IVO69_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7399355.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IVO69_000551"
     CDS             22362..23237
                     /locus_tag="IVO69_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7399356.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IVO69_000552"
     CDS             complement(23389..24231)
                     /locus_tag="IVO69_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7399357.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IVO69_000553"
     CDS             complement(24323..26200)
                     /locus_tag="IVO69_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7399358.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IVO69_000554"
     CDS             complement(26197..27618)
                     /locus_tag="IVO69_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7399359.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IVO69_000555"
     CDS             complement(27621..31010)
                     /locus_tag="IVO69_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7399360.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IVO69_000556"
     CDS             31267..32658
                     /locus_tag="IVO69_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7399361.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IVO69_000557"
     CDS             complement(32731..33378)
                     /locus_tag="IVO69_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7399362.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IVO69_000558"
     CDS             complement(33386..34072)
                     /locus_tag="IVO69_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399363.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IVO69_000559"
     CDS             complement(34078..35151)
                     /locus_tag="IVO69_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7399364.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IVO69_000560"
     CDS             35373..36047
                     /locus_tag="IVO69_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7399365.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IVO69_000561"
     CDS             36052..37392
                     /locus_tag="IVO69_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7399366.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IVO69_000562"
     CDS             complement(37541..38527)
                     /locus_tag="IVO69_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7399367.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="IVO69_000563"
     CDS             39251..40282
                     /locus_tag="IVO69_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7399368.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..>40814
                     /locus_tag="IVO69_000564"
     CDS             40391..>40814
                     /locus_tag="IVO69_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7399369.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12669 a         7413 c         7662 g        13070 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggtgccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attc
//
LOCUS       DACCPY010000012        37818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCPY010000012 DACCPY010000000
VERSION     DACCPY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.012"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(159..1469)
                     /locus_tag="IVO69_000565"
     CDS             complement(159..1469)
                     /locus_tag="IVO69_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7399370.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IVO69_000566"
     CDS             complement(1481..2899)
                     /locus_tag="IVO69_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7399371.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IVO69_000567"
     CDS             complement(3139..4707)
                     /locus_tag="IVO69_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7399372.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IVO69_000568"
     CDS             complement(4711..5121)
                     /locus_tag="IVO69_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7399373.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IVO69_000569"
     CDS             complement(5118..6041)
                     /locus_tag="IVO69_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7399374.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IVO69_000570"
     CDS             complement(6038..6916)
                     /locus_tag="IVO69_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7399375.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IVO69_000571"
     CDS             complement(6903..8240)
                     /locus_tag="IVO69_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7399376.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IVO69_000572"
     CDS             complement(8243..9346)
                     /locus_tag="IVO69_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7399377.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IVO69_000573"
     CDS             complement(9420..10928)
                     /locus_tag="IVO69_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7399378.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IVO69_000574"
     CDS             complement(10894..11586)
                     /locus_tag="IVO69_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7399379.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IVO69_000575"
     CDS             complement(11608..12582)
                     /locus_tag="IVO69_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399380.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IVO69_000576"
     CDS             12882..13754
                     /locus_tag="IVO69_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7399381.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IVO69_000577"
     CDS             complement(14212..16410)
                     /locus_tag="IVO69_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399382.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IVO69_000578"
     CDS             16642..17634
                     /locus_tag="IVO69_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7399383.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IVO69_000579"
     CDS             17694..19115
                     /locus_tag="IVO69_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7399384.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IVO69_000580"
     CDS             19108..20607
                     /locus_tag="IVO69_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7399385.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IVO69_000581"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IVO69_000581"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7399386.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IVO69_000582"
     CDS             22199..22882
                     /locus_tag="IVO69_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7399387.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IVO69_000583"
     CDS             22884..23720
                     /locus_tag="IVO69_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7399388.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IVO69_000584"
     CDS             23731..25485
                     /locus_tag="IVO69_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399389.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IVO69_000585"
     CDS             complement(25761..26102)
                     /locus_tag="IVO69_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7399390.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IVO69_000586"
     CDS             complement(26170..28341)
                     /locus_tag="IVO69_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7399391.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IVO69_000587"
     CDS             28552..29409
                     /locus_tag="IVO69_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7399392.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IVO69_000588"
     CDS             complement(29480..30337)
                     /locus_tag="IVO69_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7399393.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IVO69_000589"
     CDS             complement(30322..33024)
                     /locus_tag="IVO69_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7399394.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IVO69_000590"
     CDS             complement(33037..34326)
                     /locus_tag="IVO69_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7399395.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IVO69_000591"
     CDS             34476..35522
                     /locus_tag="IVO69_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7399396.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IVO69_000592"
     CDS             35524..37677
                     /locus_tag="IVO69_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7399397.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11174 a         7789 c         6725 g        12130 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatca
//
LOCUS       DACCPY010000013        37599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCPY010000013 DACCPY010000000
VERSION     DACCPY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.013"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..73
                     /locus_tag="IVO69_000593"
     CDS             <1..73
                     /locus_tag="IVO69_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7399398.1"
                     /translation="AVSRLAGNDYAAIGEIFTIARPE"
     gene            89..295
                     /locus_tag="IVO69_000594"
     CDS             89..295
                     /locus_tag="IVO69_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399399.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            836..1966
                     /locus_tag="IVO69_000595"
     CDS             836..1966
                     /locus_tag="IVO69_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7399400.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            2058..2813
                     /locus_tag="IVO69_000596"
     CDS             2058..2813
                     /locus_tag="IVO69_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7399401.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2871..4910
                     /locus_tag="IVO69_000597"
     CDS             2871..4910
                     /locus_tag="IVO69_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7399402.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(5019..6170)
                     /locus_tag="IVO69_000598"
     CDS             complement(5019..6170)
                     /locus_tag="IVO69_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7399403.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(6324..6854)
                     /locus_tag="IVO69_000599"
     CDS             complement(6324..6854)
                     /locus_tag="IVO69_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399404.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6953..7303)
                     /locus_tag="IVO69_000600"
     CDS             complement(6953..7303)
                     /locus_tag="IVO69_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7399405.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7460..8989)
                     /gene="ahpF"
                     /locus_tag="IVO69_000601"
     CDS             complement(7460..8989)
                     /gene="ahpF"
                     /locus_tag="IVO69_000601"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7399406.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8999..9562)
                     /gene="ahpC"
                     /locus_tag="IVO69_000602"
     CDS             complement(8999..9562)
                     /gene="ahpC"
                     /locus_tag="IVO69_000602"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7399407.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9706..10368)
                     /locus_tag="IVO69_000603"
     CDS             complement(9706..10368)
                     /locus_tag="IVO69_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7399408.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10552..12198
                     /locus_tag="IVO69_000604"
     CDS             10552..12198
                     /locus_tag="IVO69_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7399409.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            12189..13271
                     /gene="uxuA"
                     /locus_tag="IVO69_000605"
     CDS             12189..13271
                     /gene="uxuA"
                     /locus_tag="IVO69_000605"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7399410.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(13379..14932)
                     /locus_tag="IVO69_000606"
                     /pseudo
     CDS             complement(13379..14932)
                     /locus_tag="IVO69_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            15080..16221
                     /locus_tag="IVO69_000607"
                     /pseudo
     CDS             15080..16221
                     /locus_tag="IVO69_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(16347..17426)
                     /locus_tag="IVO69_000608"
     CDS             complement(16347..17426)
                     /locus_tag="IVO69_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7399411.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(17529..17969)
                     /locus_tag="IVO69_000609"
     CDS             complement(17529..17969)
                     /locus_tag="IVO69_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7399412.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(18026..18436)
                     /locus_tag="IVO69_000610"
     CDS             complement(18026..18436)
                     /locus_tag="IVO69_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7399413.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18616..19050)
                     /locus_tag="IVO69_000611"
     CDS             complement(18616..19050)
                     /locus_tag="IVO69_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7399414.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(19150..19998)
                     /locus_tag="IVO69_000612"
     CDS             complement(19150..19998)
                     /locus_tag="IVO69_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7399415.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            20387..22153
                     /locus_tag="IVO69_000613"
     CDS             20387..22153
                     /locus_tag="IVO69_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7399416.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            22255..23514
                     /locus_tag="IVO69_000614"
     CDS             22255..23514
                     /locus_tag="IVO69_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7399417.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23661..24962
                     /locus_tag="IVO69_000615"
     CDS             23661..24962
                     /locus_tag="IVO69_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399418.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24965..25816
                     /locus_tag="IVO69_000616"
     CDS             24965..25816
                     /locus_tag="IVO69_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399419.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25829..26644
                     /locus_tag="IVO69_000617"
     CDS             25829..26644
                     /locus_tag="IVO69_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7399420.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26795..27961
                     /locus_tag="IVO69_000618"
     CDS             26795..27961
                     /locus_tag="IVO69_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7399421.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            28191..28838
                     /locus_tag="IVO69_000619"
     CDS             28191..28838
                     /locus_tag="IVO69_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7399422.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28922..29866
                     /locus_tag="IVO69_000620"
     CDS             28922..29866
                     /locus_tag="IVO69_000620"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7399423.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            30001..31452
                     /locus_tag="IVO69_000621"
     CDS             30001..31452
                     /locus_tag="IVO69_000621"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7399424.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            31473..32309
                     /locus_tag="IVO69_000622"
     CDS             31473..32309
                     /locus_tag="IVO69_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7399425.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            32309..32968
                     /locus_tag="IVO69_000623"
     CDS             32309..32968
                     /locus_tag="IVO69_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7399426.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            33094..33783
                     /locus_tag="IVO69_000624"
     CDS             33094..33783
                     /locus_tag="IVO69_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399427.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33789..34745
                     /locus_tag="IVO69_000625"
     CDS             33789..34745
                     /locus_tag="IVO69_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7399428.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34789..35301
                     /locus_tag="IVO69_000626"
     CDS             34789..35301
                     /locus_tag="IVO69_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7399429.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35557..36105)
                     /locus_tag="IVO69_000627"
     CDS             complement(35557..36105)
                     /locus_tag="IVO69_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7399430.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(36194..37375)
                     /locus_tag="IVO69_000628"
     CDS             complement(36194..37375)
                     /locus_tag="IVO69_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7399431.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT        12605 a         6784 c         7148 g        11062 t
ORIGIN      
        1 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
       61 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
      121 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
      181 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
      241 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
      301 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
      361 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
      421 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
      481 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
      541 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
      601 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      661 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      721 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      781 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
      841 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
      901 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
      961 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
     1021 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
     1081 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
     1141 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
     1201 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
     1261 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
     1321 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
     1381 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
     1441 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
     1501 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
     1561 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
     1621 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
     1681 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
     1741 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
     1801 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
     1861 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
     1921 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
     1981 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
     2041 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
     2101 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
     2161 agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta aatgtggctt
     2221 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
     2281 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
     2341 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
     2401 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
     2461 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
     2521 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
     2581 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
     2641 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
     2701 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
     2761 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
     2821 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
     2881 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
     2941 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
     3001 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
     3061 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
     3121 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
     3181 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
     3241 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
     3301 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
     3361 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
     3421 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
     3481 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
     3541 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
     3601 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
     3661 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
     3721 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
     3781 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
     3841 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
     3901 aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga ggaaaagctg
     3961 gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
     4021 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
     4081 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
     4141 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
     4201 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat caagcaacgc
     4261 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
     4321 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
     4381 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
     4441 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
     4501 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
     4561 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
     4621 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
     4681 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
     4741 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
     4801 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
     4861 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
     4921 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
     4981 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
     5041 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
     5101 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
     5161 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
     5221 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
     5281 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
     5341 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
     5401 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
     5461 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
     5521 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
     5581 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
     5641 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
     5701 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
     5761 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
     5821 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
     5881 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
     5941 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
     6001 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
     6061 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
     6121 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
     6181 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
     6241 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg
     6301 tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg
     6361 ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc
     6421 aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta
     6481 taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg
     6541 atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca
     6601 tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa
     6661 cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga
     6721 taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca
     6781 atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat attttgttta
     6841 atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt
     6901 accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc
     6961 tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac
     7021 gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg
     7081 taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca
     7141 cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga
     7201 tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat
     7261 aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt
     7321 tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
     7381 aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac
     7441 tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag
     7501 tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag
     7561 ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg
     7621 gattcatttc gatcgttcca tctagccaat ccgtattagg gagtaagccg actagaatga
     7681 atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag
     7741 attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat
     7801 tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt
     7861 ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat
     7921 taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc
     7981 aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt
     8041 gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa
     8101 tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga
     8161 tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag
     8221 aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag
     8281 cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg
     8341 gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca
     8401 aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt
     8461 ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga
     8521 acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca
     8581 atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca
     8641 atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc
     8701 cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag
     8761 caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg
     8821 aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag
     8881 ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa
     8941 tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct
     9001 aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg
     9061 ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca
     9121 gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt
     9181 ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa
     9241 aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag
     9301 catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga
     9361 cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag
     9421 gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc cccataagat
     9481 ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga
     9541 tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat ttataattat
     9601 tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca
     9661 aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata
     9721 ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc
     9781 cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg
     9841 ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg
     9901 ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc
     9961 gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt
    10021 gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa
    10081 tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt
    10141 ttctgcagaa acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac
    10201 gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag
    10261 atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat
    10321 ttgtttacga atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc
    10381 aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca
    10441 cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc
    10501 ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat
    10561 ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat
    10621 tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt
    10681 ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc
    10741 gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct
    10801 taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc
    10861 aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac
    10921 tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt
    10981 actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc
    11041 gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt
    11101 tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca
    11161 caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa
    11221 ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca
    11281 tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca
    11341 aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca
    11401 tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc caatggacgt
    11461 ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg caacggtaga taaagcagat
    11521 acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt
    11581 ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt
    11641 tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca
    11701 aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat tcctaatatg
    11761 cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt
    11821 aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact
    11881 ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg
    11941 agtccagacc cactattgac agaccttcag cataagcttt ccgatattca actgggtacg
    12001 caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt
    12061 ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg
    12121 gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
    12181 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
    12241 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
    12301 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
    12361 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
    12421 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
    12481 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
    12541 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
    12601 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat
    12661 gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg
    12721 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
    12781 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag
    12841 ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt
    12901 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
    12961 ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta
    13021 aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag
    13081 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
    13141 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
    13201 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
    13261 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
    13321 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
    13381 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
    13441 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
    13501 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
    13561 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
    13621 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
    13681 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
    13741 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
    13801 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa
    13861 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
    13921 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
    13981 atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
    14041 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
    14101 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
    14161 aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt
    14221 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
    14281 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
    14341 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
    14401 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
    14461 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
    14521 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
    14581 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
    14641 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
    14701 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
    14761 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
    14821 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
    14881 tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg
    14941 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
    15001 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
    15061 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
    15121 aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg
    15181 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
    15241 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
    15301 ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
    15361 cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
    15421 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
    15481 atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga
    15541 gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
    15601 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
    15661 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa
    15721 acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg
    15781 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
    15841 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
    15901 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
    15961 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
    16021 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
    16081 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
    16141 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
    16201 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
    16261 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
    16321 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
    16381 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
    16441 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
    16501 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
    16561 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
    16621 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
    16681 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
    16741 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
    16801 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
    16861 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
    16921 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
    16981 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
    17041 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
    17101 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
    17161 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
    17221 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
    17281 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
    17341 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
    17401 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
    17461 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
    17521 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
    17581 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
    17641 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
    17701 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
    17761 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
    17821 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
    17881 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
    17941 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
    18001 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
    18061 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
    18121 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
    18181 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
    18241 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
    18301 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
    18361 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
    18421 aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca
    18481 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
    18541 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata
    18601 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac
    18661 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
    18721 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
    18781 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
    18841 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
    18901 cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
    18961 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
    19021 tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
    19081 ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta
    19141 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
    19201 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
    19261 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
    19321 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
    19381 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
    19441 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
    19501 aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
    19561 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
    19621 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
    19681 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac
    19741 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
    19801 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc
    19861 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
    19921 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
    19981 ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt
    20041 ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat
    20101 tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac
    20161 tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga
    20221 agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa
    20281 attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct
    20341 atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc
    20401 aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat
    20461 tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata
    20521 taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac
    20581 caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc
    20641 gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc
    20701 ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca
    20761 aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc
    20821 cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag
    20881 taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt
    20941 ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac
    21001 atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa
    21061 aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga
    21121 tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg
    21181 agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag attcttatca
    21241 gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc
    21301 attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc
    21361 aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt
    21421 cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta
    21481 tatccttgga gagatttggc attcctctca agcttggctg caaggggatg aattccatgc
    21541 agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac
    21601 agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa
    21661 cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca
    21721 aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc
    21781 ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa
    21841 atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact
    21901 tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat
    21961 agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta
    22021 tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct
    22081 tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt
    22141 gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg
    22201 tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa
    22261 aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta
    22321 gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta
    22381 acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa
    22441 aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca
    22501 ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg
    22561 atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct
    22621 tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca
    22681 gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg
    22741 tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga
    22801 aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg tttaattgca
    22861 ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat
    22921 aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca
    22981 aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat
    23041 aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc
    23101 aattatggtg tagccaaaat tcctactcta aataatggac agccttatca accgtttggc
    23161 ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg
    23221 cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc
    23281 cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct
    23341 gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt
    23401 tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa
    23461 tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa
    23521 tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc
    23581 gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca
    23641 agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa
    23701 tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag
    23761 gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc
    23821 atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta
    23881 ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt
    23941 tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc
    24001 ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac
    24061 ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca
    24121 ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag
    24181 gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag
    24241 tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta
    24301 ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca
    24361 ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat
    24421 tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt
    24481 ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta
    24541 tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac
    24601 aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc
    24661 gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac
    24721 agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg
    24781 gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc tataaactta
    24841 cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca
    24901 tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt
    24961 aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac
    25021 ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag
    25081 tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac
    25141 tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac
    25201 attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta
    25261 tacttatagt cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat
    25321 tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga
    25381 agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa
    25441 tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa
    25501 acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc
    25561 gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc
    25621 taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat
    25681 ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact
    25741 accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg
    25801 cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc
    25861 attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt
    25921 cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa
    25981 tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca
    26041 aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca
    26101 aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc
    26161 cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa
    26221 atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg
    26281 tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac
    26341 aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat
    26401 tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca
    26461 tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc
    26521 agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat
    26581 aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga
    26641 atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt
    26701 ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta
    26761 acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca
    26821 attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa
    26881 acaatacgga gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc
    26941 aatgaaagag attacaagta acgcatcttt ggcactagga gaagcctata tggatcatcg
    27001 aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag
    27061 ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca
    27121 aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg
    27181 gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga
    27241 acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga
    27301 aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta
    27361 taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag
    27421 aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact
    27481 aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga
    27541 aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt
    27601 gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat
    27661 tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga
    27721 tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg
    27781 gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta
    27841 tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga
    27901 tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata
    27961 attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc
    28021 agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt
    28081 tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct
    28141 tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa
    28201 ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca
    28261 atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg
    28321 actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa
    28381 gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat
    28441 acccagatac tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca
    28501 agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa
    28561 aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa
    28621 aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg
    28681 tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta
    28741 cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc
    28801 aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag
    28861 ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta
    28921 cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc
    28981 tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc
    29041 atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt
    29101 tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta
    29161 tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt
    29221 ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag
    29281 tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca
    29341 actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc
    29401 taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc
    29461 tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg
    29521 tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg
    29581 cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg
    29641 tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt
    29701 tgaacatctg ctatgtggcg caagcatgtt acagattggt acagagcttc ataaagaagg
    29761 gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata
    29821 tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg
    29881 tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc
    29941 gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat
    30001 atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa
    30061 ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc
    30121 gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg
    30181 ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta
    30241 gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg
    30301 cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt
    30361 ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa
    30421 tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct
    30481 ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg
    30541 attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg
    30601 caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt
    30661 tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa
    30721 acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca
    30781 cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa
    30841 gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat
    30901 tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag
    30961 cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag
    31021 aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc
    31081 atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc
    31141 cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt
    31201 gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag
    31261 atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact
    31321 catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa
    31381 gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat
    31441 ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa
    31501 taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata
    31561 acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt
    31621 ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa
    31681 aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac
    31741 aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa
    31801 aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga
    31861 tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag
    31921 cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag
    31981 tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag
    32041 atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc
    32101 ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag
    32161 aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg
    32221 ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag
    32281 aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt tttccctcat
    32341 gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt
    32401 gtcgtttctt ttaggaaatt tggtagggtt gcttttgaca gttttaggtc ttttggattg
    32461 gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact
    32521 agtcctgctg tttctgttat atttcgggtt accttatcaa ttaagtgcct taacggcttc
    32581 agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact
    32641 agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg
    32701 ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa
    32761 cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat
    32821 atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt
    32881 agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga
    32941 aaaacgttct atccatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc
    33001 atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa
    33061 tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata
    33121 gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg
    33181 atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc
    33241 cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa
    33301 ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta
    33361 aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag
    33421 gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct
    33481 gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa
    33541 gcaaagcaaa tggagcgagg cgtaaaatat tttccaaaag atcaattgta caaagaattg
    33601 aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga acggttttta
    33661 gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat
    33721 gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag
    33781 tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag
    33841 aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg
    33901 attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg
    33961 tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga
    34021 cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg
    34081 aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata
    34141 cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc
    34201 gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa
    34261 agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt
    34321 tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg
    34381 ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca
    34441 atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga
    34501 agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct
    34561 tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac
    34621 tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag
    34681 atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc
    34741 aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg
    34801 caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc
    34861 attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt
    34921 ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga
    34981 acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg tagttggaaa
    35041 gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga
    35101 aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc
    35161 caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct
    35221 gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca
    35281 tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga cattaacact
    35341 actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa
    35401 aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac
    35461 gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga
    35521 cctctcggct tatcctacga gtattattcg atacatttat atcaataatc ctttttttat
    35581 tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc
    35641 tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg
    35701 ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat
    35761 aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt
    35821 caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc
    35881 atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt
    35941 gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga
    36001 tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt
    36061 ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt
    36121 gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt
    36181 gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag
    36241 tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta
    36301 atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga
    36361 agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg
    36421 tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg
    36481 cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct
    36541 cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa
    36601 caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt
    36661 ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt
    36721 tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca aataagttca
    36781 aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg
    36841 acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga
    36901 agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta
    36961 ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag
    37021 ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa
    37081 atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg
    37141 caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca
    37201 atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct
    37261 gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca
    37321 aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat
    37381 cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta
    37441 gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc
    37501 gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac
    37561 tatcgattaa gctaatagtt tcgttttcaa catttataa
//
LOCUS       DACCPY010000014        37246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCPY010000014 DACCPY010000000
VERSION     DACCPY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.014"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            147..1016
                     /locus_tag="IVO69_000629"
     CDS             147..1016
                     /locus_tag="IVO69_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7399432.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IVO69_000630"
     CDS             1225..2250
                     /locus_tag="IVO69_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7399433.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IVO69_000631"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IVO69_000631"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7399434.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IVO69_000632"
     CDS             3333..5036
                     /locus_tag="IVO69_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7399435.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IVO69_000633"
     CDS             5038..7071
                     /locus_tag="IVO69_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7399436.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IVO69_000634"
     CDS             7082..7522
                     /locus_tag="IVO69_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7399437.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IVO69_000635"
     CDS             complement(7722..8849)
                     /locus_tag="IVO69_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399438.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IVO69_000636"
     CDS             complement(8852..9529)
                     /locus_tag="IVO69_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399439.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IVO69_000637"
     CDS             9793..11559
                     /locus_tag="IVO69_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7399440.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IVO69_000638"
     CDS             11574..12011
                     /locus_tag="IVO69_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7399441.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IVO69_000639"
     CDS             12024..13175
                     /locus_tag="IVO69_000639"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7399442.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IVO69_000640"
     CDS             complement(13237..13935)
                     /locus_tag="IVO69_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7399443.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IVO69_000641"
     CDS             complement(13928..15280)
                     /locus_tag="IVO69_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7399444.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IVO69_000642"
     CDS             complement(15503..16903)
                     /locus_tag="IVO69_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7399445.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IVO69_000643"
     CDS             17064..17801
                     /locus_tag="IVO69_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7399446.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IVO69_000644"
     CDS             17926..18405
                     /locus_tag="IVO69_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7399447.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IVO69_000645"
     CDS             18402..19169
                     /locus_tag="IVO69_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399448.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IVO69_000646"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IVO69_000646"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7399449.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IVO69_000647"
     CDS             complement(20072..20674)
                     /locus_tag="IVO69_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7399450.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IVO69_000648"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IVO69_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IVO69_000649"
     CDS             21355..21939
                     /locus_tag="IVO69_000649"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7399451.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IVO69_000650"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IVO69_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7399452.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IVO69_000651"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IVO69_000651"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7399453.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IVO69_000652"
     CDS             23840..24019
                     /locus_tag="IVO69_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399454.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IVO69_000653"
     CDS             24041..25066
                     /locus_tag="IVO69_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7399455.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IVO69_000654"
     CDS             25241..26485
                     /locus_tag="IVO69_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7399456.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IVO69_000655"
     CDS             26638..27585
                     /locus_tag="IVO69_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7399457.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IVO69_000656"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IVO69_000656"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7399458.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IVO69_000657"
     CDS             complement(28487..28729)
                     /locus_tag="IVO69_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399459.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IVO69_000658"
     tRNA            28850..28922
                     /locus_tag="IVO69_000658"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IVO69_000659"
     CDS             complement(29041..29679)
                     /locus_tag="IVO69_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7399460.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="IVO69_000660"
     CDS             complement(29802..30434)
                     /locus_tag="IVO69_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7399461.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30785..31084
                     /locus_tag="IVO69_000661"
     CDS             30785..31084
                     /locus_tag="IVO69_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7399462.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31579..33744
                     /gene="acm"
                     /locus_tag="IVO69_000662"
     CDS             31579..33744
                     /gene="acm"
                     /locus_tag="IVO69_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7399463.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34016..34501)
                     /locus_tag="IVO69_000663"
     CDS             complement(34016..34501)
                     /locus_tag="IVO69_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7399464.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            34981..35622
                     /locus_tag="IVO69_000664"
     CDS             34981..35622
                     /locus_tag="IVO69_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7399465.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35641..36276
                     /locus_tag="IVO69_000665"
     CDS             35641..36276
                     /locus_tag="IVO69_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7399466.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36294..37109
                     /locus_tag="IVO69_000666"
     CDS             36294..37109
                     /locus_tag="IVO69_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7399467.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT        12802 a         6386 c         7510 g        10548 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
    30601 aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc
    30661 gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag
    30721 atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat
    30781 cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt
    30841 aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga
    30901 aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc
    30961 aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg
    31021 aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt
    31081 ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc
    31141 taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt
    31201 tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg
    31261 ttaattaaaa gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt
    31321 taaaaattgg aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat
    31381 gcgaaaaaat aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt
    31441 ttttataaat atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg
    31501 gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa
    31561 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
    31621 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
    31681 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
    31741 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
    31801 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
    31861 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
    31921 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
    31981 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
    32041 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
    32101 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
    32161 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
    32221 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
    32281 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
    32341 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
    32401 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
    32461 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
    32521 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
    32581 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
    32641 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
    32701 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
    32761 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
    32821 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
    32881 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
    32941 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
    33001 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
    33061 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
    33121 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
    33181 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
    33241 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
    33301 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
    33361 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
    33421 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
    33481 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
    33541 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
    33601 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
    33661 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
    33721 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
    33781 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
    33841 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
    33901 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
    33961 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
    34021 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
    34081 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
    34141 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
    34201 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
    34261 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
    34321 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
    34381 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
    34441 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
    34501 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
    34561 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
    34621 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
    34681 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
    34741 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
    34801 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
    34861 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
    34921 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
    34981 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
    35041 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
    35101 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
    35161 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
    35221 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
    35281 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
    35341 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
    35401 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
    35461 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
    35521 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
    35581 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
    35641 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
    35701 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
    35761 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
    35821 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
    35881 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
    35941 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
    36001 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
    36061 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
    36121 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
    36181 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
    36241 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
    36301 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
    36361 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
    36421 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
    36481 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
    36541 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
    36601 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
    36661 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
    36721 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
    36781 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
    36841 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
    36901 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
    36961 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
    37021 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
    37081 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
    37141 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
    37201 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagttt
//
LOCUS       DACCPY010000015        37218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCPY010000015 DACCPY010000000
VERSION     DACCPY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.015"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..275
                     /locus_tag="IVO69_000667"
     CDS             <1..275
                     /locus_tag="IVO69_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP7399468.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            418..639
                     /locus_tag="IVO69_000668"
     CDS             418..639
                     /locus_tag="IVO69_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7399469.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            655..1497
                     /locus_tag="IVO69_000669"
     CDS             655..1497
                     /locus_tag="IVO69_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399470.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1560..1718
                     /locus_tag="IVO69_000670"
     CDS             1560..1718
                     /locus_tag="IVO69_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399471.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAENFRLSLKAP
                     EIRAFRRI"
     gene            1914..3113
                     /locus_tag="IVO69_000671"
     CDS             1914..3113
                     /locus_tag="IVO69_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399472.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            3135..3347
                     /locus_tag="IVO69_000672"
     CDS             3135..3347
                     /locus_tag="IVO69_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7399473.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3422..3898
                     /locus_tag="IVO69_000673"
     CDS             3422..3898
                     /locus_tag="IVO69_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7399474.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3895..5703
                     /locus_tag="IVO69_000674"
     CDS             3895..5703
                     /locus_tag="IVO69_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7399475.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5780..5995
                     /locus_tag="IVO69_000675"
     CDS             5780..5995
                     /locus_tag="IVO69_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7399476.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6087..6950
                     /locus_tag="IVO69_000676"
     CDS             6087..6950
                     /locus_tag="IVO69_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399477.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6981..7523
                     /locus_tag="IVO69_000677"
     CDS             6981..7523
                     /locus_tag="IVO69_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7399478.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            7537..7959
                     /locus_tag="IVO69_000678"
     CDS             7537..7959
                     /locus_tag="IVO69_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7399479.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7889..10288
                     /locus_tag="IVO69_000679"
     CDS             7889..10288
                     /locus_tag="IVO69_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7399480.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10320..12311
                     /locus_tag="IVO69_000680"
     CDS             10320..12311
                     /locus_tag="IVO69_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7399481.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12334..12585
                     /locus_tag="IVO69_000681"
     CDS             12334..12585
                     /locus_tag="IVO69_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7399482.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12575..13804
                     /locus_tag="IVO69_000682"
     CDS             12575..13804
                     /locus_tag="IVO69_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7399483.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13801..15882
                     /gene="topB"
                     /locus_tag="IVO69_000683"
     CDS             13801..15882
                     /gene="topB"
                     /locus_tag="IVO69_000683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7399484.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16031..19951
                     /locus_tag="IVO69_000684"
     CDS             16031..19951
                     /locus_tag="IVO69_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7399485.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19952..20896
                     /locus_tag="IVO69_000685"
     CDS             19952..20896
                     /locus_tag="IVO69_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399486.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20900..21103
                     /locus_tag="IVO69_000686"
     CDS             20900..21103
                     /locus_tag="IVO69_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399487.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21403..21639)
                     /locus_tag="IVO69_000687"
     CDS             complement(21403..21639)
                     /locus_tag="IVO69_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399488.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21822..22193)
                     /locus_tag="IVO69_000688"
     CDS             complement(21822..22193)
                     /locus_tag="IVO69_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399489.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(22190..23518)
                     /locus_tag="IVO69_000689"
     CDS             complement(22190..23518)
                     /locus_tag="IVO69_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7399490.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23479..23808)
                     /locus_tag="IVO69_000690"
     CDS             complement(23479..23808)
                     /locus_tag="IVO69_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7399491.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24066..24437)
                     /locus_tag="IVO69_000691"
     CDS             complement(24066..24437)
                     /locus_tag="IVO69_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7399492.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            24582..24767
                     /locus_tag="IVO69_000692"
     CDS             24582..24767
                     /locus_tag="IVO69_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7399493.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25710..26369
                     /gene="vanR"
                     /locus_tag="IVO69_000693"
     CDS             25710..26369
                     /gene="vanR"
                     /locus_tag="IVO69_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP7399494.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTYEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26372..27715
                     /gene="vanS"
                     /locus_tag="IVO69_000694"
     CDS             26372..27715
                     /gene="vanS"
                     /locus_tag="IVO69_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7399495.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            27886..28695
                     /gene="vanY"
                     /locus_tag="IVO69_000695"
     CDS             27886..28695
                     /gene="vanY"
                     /locus_tag="IVO69_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7399496.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28713..29540
                     /gene="vanW"
                     /locus_tag="IVO69_000696"
     CDS             28713..29540
                     /gene="vanW"
                     /locus_tag="IVO69_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7399497.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            29537..30508
                     /locus_tag="IVO69_000697"
     CDS             29537..30508
                     /locus_tag="IVO69_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7399498.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30501..31529
                     /gene="vanB"
                     /locus_tag="IVO69_000698"
     CDS             30501..31529
                     /gene="vanB"
                     /locus_tag="IVO69_000698"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7399499.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            31535..32143
                     /locus_tag="IVO69_000699"
     CDS             31535..32143
                     /locus_tag="IVO69_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7399500.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            32731..33162
                     /locus_tag="IVO69_000700"
     CDS             32731..33162
                     /locus_tag="IVO69_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7399501.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33169..33402
                     /locus_tag="IVO69_000701"
     CDS             33169..33402
                     /locus_tag="IVO69_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399502.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(33324..33566)
                     /locus_tag="IVO69_000702"
     CDS             complement(33324..33566)
                     /locus_tag="IVO69_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399503.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            33823..34026
                     /locus_tag="IVO69_000703"
     CDS             33823..34026
                     /locus_tag="IVO69_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7399504.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            34110..35303
                     /locus_tag="IVO69_000704"
     CDS             34110..35303
                     /locus_tag="IVO69_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7399505.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            35480..37183
                     /locus_tag="IVO69_000705"
                     /pseudo
     CDS             35480..37183
                     /locus_tag="IVO69_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
BASE COUNT         9826 a         9125 c         9913 g         8354 t
ORIGIN      
        1 aactcatcaa gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg
       61 agctgagaga caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg
      121 agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg
      181 atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca
      241 tatccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgat
      301 tctacagtag agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta
      361 ttgttattat aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg
      421 actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt
      481 gctagacgta ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta
      541 accttagatg agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt
      601 atattgcctg atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa
      661 atcataacaa taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa
      721 ccacaacctc atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt
      781 ttattaaaag aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat
      841 tggtgcattg acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca
      901 atccataaac aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt
      961 gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca
     1021 tttcctcttc ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca
     1081 aattcaacac atctttctca atataagagc aggttgtctc tatatgattt ggctaaagta
     1141 attctggaga ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat
     1201 attatcgcaa ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca
     1261 tatcataatg tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa
     1321 aatactcttc cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga
     1381 agttttgatt acgaggcatt taacgaatgt gttggaaaac tacttgatga aaataatata
     1441 catattcctt ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat
     1501 ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt aatgggggag gaaataatag
     1561 tgggattatt tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc
     1621 aactggcttc tagtctattt cgtactggta aagtttttgc tgaaaatttt aggttatcgc
     1681 tgaaagcccc ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt
     1741 actactttcg gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa
     1801 atacggcaca tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat
     1861 gggagaacag gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat
     1921 ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc
     1981 actacgacgg cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc
     2041 cggattggaa atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga
     2101 ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc
     2161 gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc
     2221 tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact
     2281 ataccctaaa aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg
     2341 tatgggagct tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca
     2401 aaatggccga tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg
     2461 aaaatccaac atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg
     2521 aaaatccaac gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa
     2581 taattacaga tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta
     2641 tggagggcga ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg
     2701 tacggattta tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca
     2761 ggagcattga ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca
     2821 ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca
     2881 agtacatgaa gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca
     2941 caacgaaagt ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca
     3001 ccatttccaa ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa
     3061 agcctaaatc cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa
     3121 aggaggacga ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg
     3181 acatccgctt tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg
     3241 tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg
     3301 tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct
     3361 gcggcgtctg gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg
     3421 attgcaggaa gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac
     3481 ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa
     3541 cacgctgcac cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc
     3601 caacattgag atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg
     3661 cgtggatttt accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt
     3721 caaggccaag gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct
     3781 ttccaaggag cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc
     3841 ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag
     3901 cctgacgtaa agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt
     3961 gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg
     4021 cattttgcgg atggttttgc ggcggctttt gccaacccgc tgccaagcct gcacctgttt
     4081 gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc
     4141 aagaacgtga agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc
     4201 aaagacatag cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg
     4261 gaacggctta ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg
     4321 ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg
     4381 caatgctttc cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact
     4441 ttggttctgg aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat
     4501 accattaact tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag
     4561 gacgtgctaa agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca
     4621 gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc
     4681 tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc
     4741 agtgaagccc gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt
     4801 ttggagcaga aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc
     4861 gcaggcaaga cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac
     4921 atccgggagg tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac
     4981 gaaaagacgg tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg
     5041 gctatgctcc aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc
     5101 gggcggctgc ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata
     5161 cccaatttcg ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt
     5221 ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg ccgccgagat catttcggat
     5281 aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat
     5341 gcgctgggga aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg
     5401 tcccacggat tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc
     5461 gtgatggacg gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag
     5521 aaattcgata ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat
     5581 acctttgacg tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg
     5641 caggatgaag tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa
     5701 taaacagcgg cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc
     5761 ggcagaaaga ggtaaattta tggcattttt taattccgca gttactactt tgcagactat
     5821 cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata
     5881 cgggcaggac aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg
     5941 cgtggccctg atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg
     6001 gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg
     6061 agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag
     6121 gcatcctcat ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca
     6181 aagtcggaga aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt
     6241 tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg
     6301 cccttgtcat gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact
     6361 ttgacagctc catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa
     6421 cgaacacatg gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa
     6481 gcgccggtgt gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg
     6541 aaagccaatt ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg
     6601 ttgtgggcct caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca
     6661 tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg
     6721 cggaatggcg cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag
     6781 cctttttaat catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg
     6841 cttccgacat atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct
     6901 gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc
     6961 caagcataca caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc
     7021 cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga
     7081 gcattgacga gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt
     7141 ttttgtgggc cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct
     7201 tttcattcaa gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt
     7261 tttacgggct gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg
     7321 gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac
     7381 acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc
     7441 gcgtggagct gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg
     7501 aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag
     7561 gacttaaccc atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt
     7621 ttttccgggg cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc
     7681 aacagcgcgg cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg
     7741 tacgagcgtc acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc
     7801 atccagccaa aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa
     7861 taccagatgg aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga
     7921 aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa
     7981 gggcgacggc aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga
     8041 cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag
     8101 ctatcagctt gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta
     8161 taactatgtg gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc
     8221 ggtgcagtac gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg
     8281 ggccgagtac accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa
     8341 gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt
     8401 gaagcgcatc ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt
     8461 gatggacggc tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat
     8521 attcaatttt gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc
     8581 cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc
     8641 ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct
     8701 caatacggaa agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc
     8761 gatcaagacc atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa
     8821 aaaggccgtc cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg
     8881 cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt
     8941 gacgttcctt gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca
     9001 agccgcaggc gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga
     9061 acagggcctt gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct
     9121 tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg
     9181 cgcggccatg tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa
     9241 gcaggcccgt tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag
     9301 ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga
     9361 cccggaggcc gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc
     9421 gcctaccagc aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga
     9481 taacccgctg gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg
     9541 caagaacggg ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta
     9601 ccggccctat ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa
     9661 agcgttgtta gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta
     9721 tgtgtccggc tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct
     9781 tgtgtccttt gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt
     9841 ccaagaccag atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta
     9901 ttttgccgac gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga
     9961 gatttggaag cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa
    10021 agacctgctt tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct
    10081 gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga
    10141 acagcagaag tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt
    10201 ggtgctgccc tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac
    10261 ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca
    10321 tgaaagagct gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc
    10381 tggaaaatca ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt
    10441 tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc
    10501 atgacggcca taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg
    10561 acgctgccct gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc
    10621 cggatttaca gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc
    10681 gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg
    10741 gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac
    10801 ccaatccggc aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg
    10861 aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc
    10921 ggcaggccgg aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg
    10981 acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat
    11041 ctttgcggga taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc
    11101 agaaacagca gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag
    11161 gcgcggcggc acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg
    11221 cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg
    11281 ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg
    11341 ccatgttcgg cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg
    11401 atatgttggc cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg
    11461 acaactacga ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc
    11521 atgaccccta tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg
    11581 gggaggtaca gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg
    11641 tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca
    11701 cccatacaga cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa
    11761 acttcgacct gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt
    11821 ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca
    11881 acgcctccac cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg
    11941 ccacctttgc cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg
    12001 gcggcagctc tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc
    12061 atagtggatg gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg
    12121 tttccagttc gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc
    12181 ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc
    12241 cgatttccta cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc
    12301 gtttacctta acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa
    12361 aaggacatcc agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg
    12421 gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac
    12481 atgaagccgg aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc
    12541 gcaacccctt accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc
    12601 ccgtattctc cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc
    12661 ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc
    12721 tacgggcggt ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac
    12781 cgaggccgaa aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc
    12841 gctgttcggt tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg
    12901 caccgacaaa aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa
    12961 tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac
    13021 ggttgaggta gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg
    13081 ctatgtccat tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg
    13141 cggtttttcc ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg
    13201 cggcggggcc agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg
    13261 cagcgccgcg aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt
    13321 ttatctgatt attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt
    13381 ggatgaagcc gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt
    13441 ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa
    13501 aaccaacctg accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga
    13561 agccccggag gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc
    13621 gctggcgggc ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac
    13681 caccggcagc gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga
    13741 ggaacccgaa accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga
    13801 atgacatata aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg
    13861 atcggggcca cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg
    13921 tgcatcggac atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa
    13981 tggcgctatg aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac
    14041 aagaaacagc agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt
    14101 gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg
    14161 gcgggatgcc aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt
    14221 aaggacggct ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc
    14281 ctgtgccggg ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc
    14341 ctgtaccaca agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg
    14401 gacagggacg ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg
    14461 gcgggcggcg cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc
    14521 aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg
    14581 gaacagccgc ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc
    14641 ggctataccg ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg
    14701 acctatccga gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc
    14761 cttgtgtccg gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag
    14821 tttgaccgca tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct
    14881 gaatttgtga agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg
    14941 atctgctgca agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc
    15001 acatttacct gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc
    15061 tggaaagaga ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc
    15121 gaggcggtgc gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc
    15181 tctgtcaccg agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc
    15241 gctatggaac gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc
    15301 acaccggcca cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga
    15361 aaaggcaagc agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag
    15421 gcgcttactt ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag
    15481 gggcaggccg atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg
    15541 cagacctatt cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct
    15601 atcgggacgt gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc
    15661 ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag
    15721 aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag
    15781 ggcctctatt ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc
    15841 gggggcaagt acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa
    15901 gcatagggag tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg
    15961 ggatttttgc ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag
    16021 gagtgaccgc gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca
    16081 gggcaaacaa ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct
    16141 gcgggatgtt ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg
    16201 cccggaggac agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga
    16261 gctgaatttc cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa
    16321 cgccgccctg caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac
    16381 cgaaaatgtg gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga
    16441 ctactacctg aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga
    16501 tacggcgcag tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg
    16561 ctatctggtg agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga
    16621 ggaataccgg gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca
    16681 gcagccggag gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct
    16741 ggacggcatg aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt
    16801 acaggagctt tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca
    16861 cgattacagt ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg
    16921 ctataacagg tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa
    16981 gatactggcc ccggccccgt tcaaggttaa gcagcagatg gagaaaatcg acccggccac
    17041 ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt
    17101 tccggccttt aaggtggtga cagtctttga catcagccag accgagggca aggattttcc
    17161 catgccctat atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc
    17221 gctggaacgg acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg
    17281 ccgctgcttc tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac
    17341 cctaaagacc gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc
    17401 ccccgccgag gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag
    17461 cgtggcctat accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta
    17521 tgtggccgat tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat
    17581 ccgggccacg gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca
    17641 ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc
    17701 ggccccggcg caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct
    17761 tgtgactgtg ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca
    17821 tgaggccgac gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga
    17881 caaaaccgcg ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg
    17941 gcaggatttt ggagataggg acggttccct gatcgaacat atccagagct accaccaatt
    18001 ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg
    18061 ggagcaggac aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct
    18121 gcattgcagc cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac
    18181 cctgacgccg gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg
    18241 ccccatgctc aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga
    18301 ccagagcttg caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc
    18361 agccggtatg accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac
    18421 cttttccatc taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata
    18481 cgagcgttta caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac
    18541 ggcaccgctg gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca
    18601 cccggcagat tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa
    18661 tggacaggac accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt
    18721 gcgggaaaat cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga
    18781 cggcattatc aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt
    18841 tgaggatgtg gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc
    18901 gcaaacggct gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc
    18961 catgagcttt tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga
    19021 tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa
    19081 gatagacagc ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta
    19141 tgagattacc gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg
    19201 ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca
    19261 gggcttgctt gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca
    19321 ggccgtttcc aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa
    19381 ggccgtcaat gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgaccga
    19441 aagaggcatt gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc
    19501 gttccagtcc tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat
    19561 tgaggaattg acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac
    19621 tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc
    19681 tgctgtacgc gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg
    19741 gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc
    19801 cctttccggc gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc
    19861 ctctatccgg gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg
    19921 ggaaaaatca aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct
    19981 ggccgcgctg gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat
    20041 tgacaaagac attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct
    20101 ctggatgtcc cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga
    20161 cacccgacag tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc
    20221 ctacgcggtg gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct
    20281 ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg
    20341 gctcttttat gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc
    20401 tgaccgggta ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct
    20461 gcaggaggcg ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa
    20521 agtccacgtc accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga
    20581 cagcctgaaa gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat
    20641 ctccccgcac tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct
    20701 gccctataaa tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat
    20761 taaccgggac gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca
    20821 gctatccgac agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa
    20881 tgaattggag gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat
    20941 gtaccacaat gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat
    21001 ggatggggat atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga
    21061 tgccgactat gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg
    21121 gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca
    21181 tttagaaccc ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg
    21241 ggagaagcct tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt
    21301 agacccctga aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg
    21361 atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg
    21421 gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat
    21481 gactttcttc tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta
    21541 gtcagcataa tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt
    21601 gatcgtttca tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg
    21661 attttttcta tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct
    21721 ggtaaattct acgcatttag aacagcactt cggcggggat aggataaaac acccacatac
    21781 cccgctgacc atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta
    21841 ggtttccagt atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag
    21901 caggccgcgc agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag
    21961 cagcgtttca cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg
    22021 aggacagcgg ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata
    22081 cattcttcgc cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc
    22141 gtcagcttcc gggccttgcg atattgaggg taattcattc gtggaatact catagctctt
    22201 gcgtccattc ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc
    22261 cgtattcctg catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct
    22321 cggccaatcg cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct
    22381 gtccctttag ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc
    22441 ggaattttgc cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt
    22501 aggtagaaat attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga
    22561 gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg
    22621 ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat
    22681 tgtggagctt cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca
    22741 gatcaatccg caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt
    22801 tgatacgctc tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa
    22861 agcgttcctg tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct
    22921 gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa
    22981 tggctgcttt gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat
    23041 agctcttgcc cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc
    23101 tgtcgctggt gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat
    23161 ttaccatatc cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg
    23221 tagtcagcac atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct
    23281 catgggcctg ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca
    23341 gattattgcc cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt
    23401 cataagagca ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga
    23461 tgtagtcaag ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat
    23521 ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt
    23581 cagcctcttt gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc
    23641 ccggatgtcg ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta
    23701 ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc
    23761 cggcgtcaca gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc
    23821 tttccattca ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga
    23881 cggggcaggc cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc
    23941 tccactaaca agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa
    24001 aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat
    24061 gccagttaca catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca
    24121 atcgcgccct cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc
    24181 ctttgccgca gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga
    24241 agcccgcagg ctttaataag ctgtatgaca accggcagac tgggaatggc cccgtcgttt
    24301 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
    24361 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
    24421 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
    24481 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
    24541 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
    24601 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
    24661 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
    24721 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
    24781 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
    24841 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
    24901 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
    24961 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
    25021 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
    25081 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
    25141 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
    25201 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
    25261 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
    25321 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
    25381 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
    25441 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
    25501 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
    25561 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
    25621 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
    25681 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
    25741 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
    25801 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
    25861 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
    25921 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
    25981 cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
    26041 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
    26101 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
    26161 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgt
    26221 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
    26281 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
    26341 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
    26401 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
    26461 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
    26521 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
    26581 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
    26641 ctatgccaca ccaaatgcca atacatcaaa tagttttaga tccgactttc tttatgtggt
    26701 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
    26761 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
    26821 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
    26881 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
    26941 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
    27001 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
    27061 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
    27121 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
    27181 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
    27241 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
    27301 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
    27361 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
    27421 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
    27481 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
    27541 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcggttt
    27601 aggacttgcc atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa
    27661 cacctcggat ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt
    27721 taaaacttaa atgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca
    27781 ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta
    27841 ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaaaaagc
    27901 aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg
    27961 gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga
    28021 ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc
    28081 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
    28141 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
    28201 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
    28261 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
    28321 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
    28381 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
    28441 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
    28501 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
    28561 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
    28621 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
    28681 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
    28741 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
    28801 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
    28861 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
    28921 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
    28981 gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag
    29041 acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga
    29101 tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag
    29161 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
    29221 ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
    29281 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc
    29341 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
    29401 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
    29461 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
    29521 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
    29581 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
    29641 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg
    29701 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
    29761 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
    29821 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
    29881 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
    29941 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
    30001 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
    30061 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
    30121 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
    30181 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc gcggagcgct
    30241 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
    30301 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
    30361 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
    30421 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
    30481 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
    30541 cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg
    30601 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
    30661 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
    30721 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
    30781 tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg tttgtattgt
    30841 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
    30901 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa atgattgata
    30961 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
    31021 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
    31081 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
    31141 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
    31201 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
    31261 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
    31321 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
    31381 tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt tttacatcgt
    31441 acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca
    31501 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
    31561 tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg
    31621 aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc
    31681 tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg
    31741 atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa
    31801 gctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta
    31861 tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct
    31921 ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca
    31981 tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat
    32041 catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga
    32101 tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg
    32161 tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt
    32221 ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga
    32281 aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg
    32341 cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata
    32401 ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa
    32461 aaaagccttg cccctccaca gggctattcc ggttatgagt atgtactata ccatgtaaat
    32521 cagcagcata gcaaagcatt ttctcgcccg gtaggtttgc gacctgccgg gcgatttttg
    32581 tcgtcttttg cggcccggct gtccccaaaa aaattttttg agaaattttc aaaaaggagg
    32641 tatttagcgg gtagctggcg gccttgtcaa agtcttgtta gcggaaaggg agagaaccac
    32701 ctaagtcccc cacagaaagg gggtgagaag atggatgcta tcccccgcga ctatgaagcg
    32761 cggtgcatgt tcgatgcttt ttgcaagacg gtactgcgta acgaggccaa aagctacttg
    32821 gctgaaatga agcgccgccg tgaccgtgaa gtgtcactaa gctctttgtc acaggcagat
    32881 ttggacaagc tctgtacagt agatcactac cccagcgaca gctttacgtt ttcgtcgcat
    32941 ggctatgacc tacatatcaa caatgagctt gttgccgaag cctttgccgc gctgcccagc
    33001 atggagcaga gtattttaat cctgcactgt gttctggata tggcagatgg tgagatcggc
    33061 ggccttgtgg gaatgtcccg gagtgccgtt cagcggcaca ggacaaatac cttatcggag
    33121 ctgcggaaac agctaaaggc gttgatgccg aagggaggtt agacgcggat gaagaacccg
    33181 aactacaagg gcattgagct tttgcccctt tcggtgatcg aggccgcccg cgctggggat
    33241 gccggagccg tagagcgcgt tttgcagtat tacagcggtt acatgaacaa gctgtgtacc
    33301 cggacgctct atgacgaaaa cggctatccg catgtgtgtc tggacgaggc catgaagcgc
    33361 cgtctggaaa tcaagctcat ccacgctatc gtcacgaact gacgaaccgg ccccggcagc
    33421 atgaaacggc ttaccctttc cccgtttcgg tgttttcccg gtggcctgat cgcaccttga
    33481 taaatatgcc cgcaagataa cggcggcgca tcatagggca tacggacaca ttctgtctgt
    33541 gccgagccgg acggctggtg cgccatgata cttttcccta cgggaaaagc ggagcgagga
    33601 acaccacgcc gcaatgtgag cgtggcggct tgcgggcgac gacgtacagg caggactatg
    33661 atactccctt atagccacag tccgagcgtt aaaagcgtcg caggcaatgg gtagggccgc
    33721 gtgagaacca cgcgggggtg aaagtcccgt ggagctgtgc cagcagccgt ccgtctatgc
    33781 ccctaaatca cacaaacctt tgaatttgtg aaagggggct gcattatgaa ccatgatgtg
    33841 cctatttggg aaaaatacac gctgacgatt gaggaagcgt ctaaatattt ccgtatcgga
    33901 gaaaacaaat tgcgtaagct ggccgaggaa aatccggcct ctggctgggt cattatgaat
    33961 ggcaaccgca tccagatcaa acgcaagcag tttgagaaaa taattgacac tttggacaca
    34021 atctaatagt gaaaaagggc cgcagatatg ttataataat ttataagcat atatgcggct
    34081 ctttccgtca tggaaaggag catgacgaga tgtcagaaaa aagacgagac agcaaaaatc
    34141 gcgttttgcg gtctggagag agccagagaa aagacggacg atatgcttac aaatatgttg
    34201 atacctttgg aaaaccgcag tttgtttact catggaagtt agtgcctacg gataagaccc
    34261 cggcaggtaa acgggaggat atttcactcc gggaaaagga gaaagaaatc cagaaagacc
    34321 ttgacgacgg gatagacacc atcggcaaga aaatgaccgt ctgccagctc tacgccaagc
    34381 agatcaggca ccggggcaat gtgaagcaca acaccaaact gggccgggaa cggctgatgc
    34441 gtattctgga acaagatcgg ctggggagct gcccgattga cagtgtgaag ctgtccgacg
    34501 caaaggaatg ggcgcttcgg atgaaagaaa agggcctttc ttataagacc atcaataacg
    34561 acaagcgttc cctgaaagcg gctttttaca ccgccataca ggacgattgc atccgaaaaa
    34621 acccgtttga cttccagctt agtgacgttc tggacgacga tacggaacca aaggtgcctc
    34681 ttacaccagc acaggaagaa agttttctgt cctttattca aggtgataag gtttaccaaa
    34741 agcattatga cgcaatcgtt attctgctgg ggacagggct tcgcatttcg gaactgtgcg
    34801 gactgacgga caaagatttg gattttgaaa accgggtaat catcgtgagc caccagcttt
    34861 tgagaaatac cggcgtcggt tactatattg acgaacccaa gacccaaagc ggcgtcagaa
    34921 aaatccccat gaatgaggaa gtgtatcagg cgttccagcg agtgataaaa aaccgcaaag
    34981 gtgcaaagcc gttcattatt gacgggtatg ccaatttcct gtttctcaaa cagaacggct
    35041 atcccatgac ggcggtagat tatggcggga tgtttggcag gcttgtgaag aagtacaaca
    35101 agagccatga agaagcgttg ccgaaaacga cgacgcccca tgctatgcgt catacattct
    35161 gcacccggtt agccaatgcg ggaatgaacc ccaaagcctt gcagtacatt atgggacatt
    35221 ccaatatcac catgacgctg aactattatg cacacgccac attcgattcc gcaagggcag
    35281 aaatggaacg gctggcagcc tgacacaagg ccggttttac tactccattt actacttttg
    35341 agggggaaat ggtgagaggt aataagaaca tttgtaagta tctaacaata tcaaaaatgc
    35401 cggaaaagcc cggaaacacg ggctataccg acattcaaga acttctaaaa agataatcta
    35461 aaatcccata taattttctg cgcaaaaatt ggaagtggac atatcaaatg tagatgagtt
    35521 tcgtaaaact tttatcgctt ttgatgtgga aacaactggc ctaaattctt cttcagatag
    35581 aattgtagag attggtgcgg tgctatttaa agaaggagtt ccaattcaat cattcggaac
    35641 tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg gcaataaata atataactaa
    35701 tgaaatgcta aactctgctc caaatgagca ggaagtttac ccccagttgg tagaattttt
    35761 aggagatgca ctagatggaa aaatcactat gtgtgcacac aatgcaagtt tcgattttgg
    35821 atttttaagt aacacatttt ctagacttgg gtatgatgct aaaattaagt atgttgatac
    35881 tctgagtctc tctagaaaac atataaaagg cattgtaaat cataagcaag agactgtagc
    35941 ggaatatttc ggattaataa atgacactgc ccatagagcg gaatcagatg cagatatatg
    36001 cggaaagatt ttttggggtg ttttagatat tattgaggag tcgttggaag aacaaaaaaa
    36061 gcaaatagaa aagatgactc cagacataaa agaacttgaa gcttgtgctt ttattcaaga
    36121 tattattgag aaaaaaggat gcgacaccag ttggatcagg tatcgaaaaa acagtaataa
    36181 ttatgtcgat gtaacttgtc tatatacatt cttaaagttt aagtttgcta agaagggaaa
    36241 ttacatcatt attagaaaag atgctacaaa tggaattgat ttacctatag aaccttgtac
    36301 tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt cgtagcccct ttgacttaac
    36361 gcctctttca aattatattt tcagtgctta tagcgattgt tataattcta tgaatgaata
    36421 tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa agccttagta tgctgaaatc
    36481 tatttcaaaa tcagcgatga aagagttatt attcgatgca aaaaatcgtg gatatgatga
    36541 gactgaagtt aacctaaata ttgaaaaagt gatatctaga gaagatgtaa ttattaacgg
    36601 gttgcataat cgagtttcat taaacaagat acgaaatata gggaattggg agaaaggatt
    36661 tgatgcaggt tatccatatt gggaacgtgg agaaactgct aggaaaaatg gcaaagtaga
    36721 tgaagctatt gcactttttg ataaagctcg atataacggc tatgatgctc ctgcgctata
    36781 tgattcttat gctaaagctt atcgaaaaat taaggattac gataatgaaa ttctcattct
    36841 tgatgaagga attatgcgta aaacacggca cgatgttggt actttagagg ctcgtaggga
    36901 taaggcaatt aagctattat ttgctaaaca agaggcagag cggatagcta aggaaaaatc
    36961 tgattttcta aaagcgaaca aaaaagagga tgcgattagt gagcctagca agaagcgtgg
    37021 aagacagatt attcaaatga ctgatgatgg aacaataata aaagaattcg atactatagc
    37081 ttctgctgta aaagaagttg gcgttaactc aaaaagtatt agggatgctg caaatggagt
    37141 tcaaaaacat gctggaggat actactggaa atacaaagat tagcagataa tctaagataa
    37201 cctaggatct tcttaata
//
LOCUS       DACCPY010000016        36581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCPY010000016 DACCPY010000000
VERSION     DACCPY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.016"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVO69_000706"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVO69_000706"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7399506.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVO69_000707"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVO69_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7399507.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVO69_000708"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVO69_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7399508.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVO69_000709"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVO69_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7399509.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVO69_000710"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVO69_000710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7399510.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVO69_000711"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVO69_000711"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7399511.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVO69_000712"
     CDS             complement(8053..8523)
                     /locus_tag="IVO69_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7399512.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVO69_000713"
     CDS             complement(8569..8994)
                     /locus_tag="IVO69_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7399513.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVO69_000714"
     CDS             complement(9061..9330)
                     /locus_tag="IVO69_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7399514.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVO69_000715"
     CDS             complement(9341..10936)
                     /locus_tag="IVO69_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7399515.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVO69_000716"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVO69_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7399516.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVO69_000717"
     CDS             complement(14489..15049)
                     /locus_tag="IVO69_000717"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7399517.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVO69_000718"
     CDS             15402..16376
                     /locus_tag="IVO69_000718"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7399518.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVO69_000719"
     CDS             16546..17946
                     /locus_tag="IVO69_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7399519.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVO69_000720"
     CDS             complement(18024..18746)
                     /locus_tag="IVO69_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7399520.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVO69_000721"
     CDS             complement(18825..19331)
                     /locus_tag="IVO69_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7399521.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVO69_000722"
     CDS             19454..21253
                     /locus_tag="IVO69_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7399522.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22859)
                     /locus_tag="IVO69_000723"
     CDS             complement(21321..22859)
                     /locus_tag="IVO69_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399523.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="IVO69_000724"
     CDS             complement(23114..23623)
                     /gene="mreD"
                     /locus_tag="IVO69_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7399524.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="IVO69_000725"
     CDS             complement(23627..24481)
                     /gene="mreC"
                     /locus_tag="IVO69_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7399525.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24636..25274)
                     /locus_tag="IVO69_000726"
     CDS             complement(24636..25274)
                     /locus_tag="IVO69_000726"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7399526.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25275..25925)
                     /locus_tag="IVO69_000727"
     CDS             complement(25275..25925)
                     /locus_tag="IVO69_000727"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7399527.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25938..26837)
                     /gene="rsgA"
                     /locus_tag="IVO69_000728"
     CDS             complement(25938..26837)
                     /gene="rsgA"
                     /locus_tag="IVO69_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7399528.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27002..29071)
                     /gene="pknB"
                     /locus_tag="IVO69_000729"
     CDS             complement(27002..29071)
                     /gene="pknB"
                     /locus_tag="IVO69_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7399529.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29068..29808)
                     /locus_tag="IVO69_000730"
     CDS             complement(29068..29808)
                     /locus_tag="IVO69_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7399530.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29821..31179)
                     /gene="rsmB"
                     /locus_tag="IVO69_000731"
     CDS             complement(29821..31179)
                     /gene="rsmB"
                     /locus_tag="IVO69_000731"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7399531.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31179..32117)
                     /locus_tag="IVO69_000732"
     CDS             complement(31179..32117)
                     /locus_tag="IVO69_000732"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7399532.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32110..32601)
                     /gene="def"
                     /locus_tag="IVO69_000733"
     CDS             complement(32110..32601)
                     /gene="def"
                     /locus_tag="IVO69_000733"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7399533.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32616..35024)
                     /gene="priA"
                     /locus_tag="IVO69_000734"
     CDS             complement(32616..35024)
                     /gene="priA"
                     /locus_tag="IVO69_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7399534.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35277..35582)
                     /locus_tag="IVO69_000735"
     CDS             complement(35277..35582)
                     /locus_tag="IVO69_000735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7399535.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35587..36201)
                     /gene="gmk"
                     /locus_tag="IVO69_000736"
     CDS             complement(35587..36201)
                     /gene="gmk"
                     /locus_tag="IVO69_000736"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7399536.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9896 a         7999 c         6705 g        11981 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    21961 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22021 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22081 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22141 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22201 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22261 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22321 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22381 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22441 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    22501 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    22561 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    22621 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    22681 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    22741 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    22801 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    22861 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    22921 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    22981 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23041 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23101 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23161 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23221 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23281 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23341 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23401 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    23461 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    23521 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    23581 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    23641 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    23701 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    23761 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    23821 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    23881 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    23941 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24001 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24061 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24121 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24181 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24241 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24301 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24361 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24421 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    24481 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    24541 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    24601 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    24661 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    24721 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    24781 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    24841 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    24901 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    24961 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25021 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25081 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcgcccact
    25141 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25201 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25261 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25321 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25381 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25441 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    25501 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    25561 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    25621 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    25681 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    25741 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    25801 tttgggacaa attggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    25861 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    25921 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    25981 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26041 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26101 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26161 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26221 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26281 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26341 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26401 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    26461 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    26521 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    26581 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    26641 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    26701 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    26761 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    26821 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    26881 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    26941 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    27001 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    27061 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    27121 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    27181 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    27241 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    27301 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    27361 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    27421 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    27481 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    27541 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    27601 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    27661 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    27721 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    27781 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    27841 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    27901 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    27961 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    28021 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    28081 caggcagaac ttcttcctcc tctggcaagg tttcgttttc tggcttgtct tttggcagag
    28141 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    28201 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    28261 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    28321 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    28381 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    28441 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    28501 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcccagca
    28561 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    28621 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    28681 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    28741 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    28801 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    28861 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    28921 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    28981 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    29041 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    29101 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    29161 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    29221 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    29281 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    29341 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    29401 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    29461 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    29521 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    29581 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    29641 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    29701 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    29761 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    29821 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    29881 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    29941 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    30001 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    30061 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    30121 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    30181 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    30241 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    30301 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    30361 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    30421 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    30481 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    30541 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    30601 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    30661 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    30721 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    30781 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    30841 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    30901 ttggtataca gaaagataaa gaagcgtctt gacccagtca tccacttttt ttgctttagc
    30961 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    31021 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    31081 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    31141 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    31201 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    31261 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    31321 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    31381 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    31441 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    31501 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    31561 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    31621 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    31681 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    31741 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    31801 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    31861 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    31921 attttctctg gctgcagcac acggataccg tgcttcagag ccgcttcttt taccggtgtc
    31981 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    32041 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    32101 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    32161 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    32221 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    32281 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    32341 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    32401 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    32461 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    32521 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    32581 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    32641 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    32701 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    32761 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    32821 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    32881 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    32941 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagctaattc tatggcgtaa
    33001 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    33061 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    33121 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    33181 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    33241 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    33301 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    33361 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    33421 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    33481 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    33541 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    33601 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    33661 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    33721 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    33781 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    33841 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    33901 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    33961 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    34021 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    34081 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    34141 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    34201 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    34261 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    34321 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    34381 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    34441 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    34501 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    34561 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    34621 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    34681 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    34741 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    34801 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    34861 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    34921 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    34981 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    35041 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    35101 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    35161 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    35221 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    35281 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    35341 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    35401 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    35461 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    35521 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    35581 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    35641 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    35701 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttc
    35761 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    35821 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    35881 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgatata
    35941 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    36001 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    36061 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    36121 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    36181 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    36241 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    36301 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    36361 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    36421 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    36481 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgtaagc
    36541 gccccataga acagtacctg tacgtcaaat aacccgacct a
//
LOCUS       DACCPY010000017        35435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCPY010000017 DACCPY010000000
VERSION     DACCPY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.017"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..966)
                     /locus_tag="IVO69_000737"
     CDS             complement(<1..966)
                     /locus_tag="IVO69_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7399537.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTH"
     gene            complement(1082..1717)
                     /locus_tag="IVO69_000738"
     CDS             complement(1082..1717)
                     /locus_tag="IVO69_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7399538.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1800..2222)
                     /locus_tag="IVO69_000739"
     CDS             complement(1800..2222)
                     /locus_tag="IVO69_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7399539.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2240..2560)
                     /locus_tag="IVO69_000740"
     CDS             complement(2240..2560)
                     /locus_tag="IVO69_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7399540.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2860..3597
                     /locus_tag="IVO69_000741"
     CDS             2860..3597
                     /locus_tag="IVO69_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7399541.1"
                     /translation="MNTPQIFNFEQNEVRTFLENDIPYFVANDVAKTLGYKNPSDATN
                     KHCKKAVKTWGSDSLGRRQSFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLE"
     gene            3621..3824
                     /locus_tag="IVO69_000742"
     CDS             3621..3824
                     /locus_tag="IVO69_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399542.1"
                     /translation="MTDIAEITQRDREKIKAYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            3840..4160
                     /locus_tag="IVO69_000743"
     CDS             3840..4160
                     /locus_tag="IVO69_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7399543.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            4165..4344
                     /locus_tag="IVO69_000744"
     CDS             4165..4344
                     /locus_tag="IVO69_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399544.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(4464..5042)
                     /locus_tag="IVO69_000745"
     CDS             complement(4464..5042)
                     /locus_tag="IVO69_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399545.1"
                     /translation="MVVFSMNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEK
                     VINDLKRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDI
                     LEENFRIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRI
                     FSLVIWVTSLFAQETISQILSFVITHFFKGQK"
     gene            5081..5779
                     /locus_tag="IVO69_000746"
     CDS             5081..5779
                     /locus_tag="IVO69_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7399546.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            5961..6296
                     /locus_tag="IVO69_000747"
     CDS             5961..6296
                     /locus_tag="IVO69_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399547.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            6529..7470
                     /locus_tag="IVO69_000748"
     CDS             6529..7470
                     /locus_tag="IVO69_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7399548.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            7472..8362
                     /locus_tag="IVO69_000749"
     CDS             7472..8362
                     /locus_tag="IVO69_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP7399549.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            8405..9205
                     /locus_tag="IVO69_000750"
     CDS             8405..9205
                     /locus_tag="IVO69_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7399550.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            9220..10071
                     /locus_tag="IVO69_000751"
     CDS             9220..10071
                     /locus_tag="IVO69_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7399551.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            10068..10385
                     /locus_tag="IVO69_000752"
     CDS             10068..10385
                     /locus_tag="IVO69_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399552.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            10378..10560
                     /locus_tag="IVO69_000753"
     CDS             10378..10560
                     /locus_tag="IVO69_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399553.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            10568..10954
                     /locus_tag="IVO69_000754"
     CDS             10568..10954
                     /locus_tag="IVO69_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399554.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEVE"
     gene            10951..11286
                     /locus_tag="IVO69_000755"
     CDS             10951..11286
                     /locus_tag="IVO69_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399555.1"
                     /translation="MIILATDYIDSLKDEDGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSESAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            11286..11516
                     /locus_tag="IVO69_000756"
     CDS             11286..11516
                     /locus_tag="IVO69_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399556.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSAVENHLKEFRSSIDDTFRLGS"
     gene            11523..11720
                     /locus_tag="IVO69_000757"
     CDS             11523..11720
                     /locus_tag="IVO69_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7399557.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            11717..11923
                     /locus_tag="IVO69_000758"
     CDS             11717..11923
                     /locus_tag="IVO69_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399558.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLSIVDILQSAKWFDNLEEAN
                     QVSRRLGGLTQVYELITIDHEEVE"
     gene            11923..12216
                     /locus_tag="IVO69_000759"
     CDS             11923..12216
                     /locus_tag="IVO69_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399559.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVIKS"
     gene            12246..12686
                     /locus_tag="IVO69_000760"
     CDS             12246..12686
                     /locus_tag="IVO69_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7399560.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            12761..13228
                     /locus_tag="IVO69_000761"
     CDS             12761..13228
                     /locus_tag="IVO69_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7399561.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(13641..13955)
                     /locus_tag="IVO69_000762"
     CDS             complement(13641..13955)
                     /locus_tag="IVO69_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399562.1"
                     /translation="MSNGFDDLSKKLNKMAKAAQEMDGEHSVPMSELFTNDFVSANTK
                     FSSIEEFFEVSGFDCSSEESFAAIPDDQMDAFVSDNTNFDTWSDFMSAAAIKYTAKKL
                     GF"
     gene            14947..15291
                     /locus_tag="IVO69_000763"
     CDS             14947..15291
                     /locus_tag="IVO69_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7399563.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15296..15577
                     /locus_tag="IVO69_000764"
     CDS             15296..15577
                     /locus_tag="IVO69_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399564.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            15679..15993
                     /locus_tag="IVO69_000765"
     CDS             15679..15993
                     /locus_tag="IVO69_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7399565.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            15971..17665
                     /locus_tag="IVO69_000766"
     CDS             15971..17665
                     /locus_tag="IVO69_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7399566.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            17685..18863
                     /locus_tag="IVO69_000767"
     CDS             17685..18863
                     /locus_tag="IVO69_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7399567.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18826..19512
                     /locus_tag="IVO69_000768"
     CDS             18826..19512
                     /locus_tag="IVO69_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7399568.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            19512..20672
                     /locus_tag="IVO69_000769"
     CDS             19512..20672
                     /locus_tag="IVO69_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7399569.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            20682..21557
                     /locus_tag="IVO69_000770"
     CDS             20682..21557
                     /locus_tag="IVO69_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399570.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            21554..21865
                     /locus_tag="IVO69_000771"
     CDS             21554..21865
                     /locus_tag="IVO69_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399571.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21855..22208
                     /locus_tag="IVO69_000772"
     CDS             21855..22208
                     /locus_tag="IVO69_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7399572.1"
                     /translation="MANNRRLEETFNDGWLKILTQSTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            22198..22599
                     /locus_tag="IVO69_000773"
     CDS             22198..22599
                     /locus_tag="IVO69_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399573.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            22592..22996
                     /locus_tag="IVO69_000774"
     CDS             22592..22996
                     /locus_tag="IVO69_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399574.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            23008..23616
                     /locus_tag="IVO69_000775"
     CDS             23008..23616
                     /locus_tag="IVO69_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7399575.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            23636..23998
                     /locus_tag="IVO69_000776"
     CDS             23636..23998
                     /locus_tag="IVO69_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399576.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            24001..24183
                     /locus_tag="IVO69_000777"
     CDS             24001..24183
                     /locus_tag="IVO69_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399577.1"
                     /translation="MPNLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            24200..27520
                     /locus_tag="IVO69_000778"
     CDS             24200..27520
                     /locus_tag="IVO69_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7399578.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALDMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEKFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFKGIWSKF
                     TDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPHFKLSGKFNPLKGKIPTLGVDWYAKGSVFNSPNIIGIGEAG
                     PEVALPLKRSVLQEIGDRILSSTSVSSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            27571..28308
                     /locus_tag="IVO69_000779"
     CDS             27571..28308
                     /locus_tag="IVO69_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7399579.1"
                     /translation="MTDCIHSIVDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            28318..30609
                     /locus_tag="IVO69_000780"
     CDS             28318..30609
                     /locus_tag="IVO69_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399580.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWILVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            30633..32831
                     /locus_tag="IVO69_000781"
     CDS             30633..32831
                     /locus_tag="IVO69_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399581.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPFQPNLLAEPYNMCREYPNENIADPTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVAMPKLFRVYQYPRSTVGACRIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSKINSQLTASINEVDTTAKGA
                     QTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVANGILQFTSCRTLVLTRLSD"
     gene            32909..33151
                     /locus_tag="IVO69_000782"
     CDS             32909..33151
                     /locus_tag="IVO69_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7399582.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            33167..33364
                     /locus_tag="IVO69_000783"
     CDS             33167..33364
                     /locus_tag="IVO69_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7399583.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            33375..34394
                     /locus_tag="IVO69_000784"
     CDS             33375..34394
                     /locus_tag="IVO69_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7399584.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGLNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            34584..34657
                     /locus_tag="IVO69_000785"
     tRNA            34584..34657
                     /locus_tag="IVO69_000785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34618..34620,aa:Ile,seq:cat)
     gene            35334..>35435
                     /locus_tag="IVO69_000786"
     CDS             35334..>35435
                     /locus_tag="IVO69_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7399585.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREY"
BASE COUNT        12487 a         5511 c         7224 g        10213 t
ORIGIN      
        1 atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt
       61 tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa
      121 aacaaaatta tgagggtttt taacttcaaa attctcgaat aattcttttt gattgatttt
      181 aaactttttt aagagatcaa tcgtatgttt atcaattgaa attttccgat ttgaagtctc
      241 gttttttgtt tttccccatt catttaattt ataattccat gttttattaa tagtgatgat
      301 ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa atcgcattcc
      361 agtagcacca gcaactaaca caagcatagg agaagagtac tgtgcagaaa ggttttcttc
      421 tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt cttttggctt
      481 caatgaatct tttcctttaa ttactgcttt tctagttgga tcaaacggaa ttaatccttc
      541 ttctacggca tctttcaacg atgctctaat atggttatta aacttaataa cggtggattt
      601 tacatggtct ttcgcatact tatttaaaaa gcgttgatat ccaactctat ctaaatcaga
      661 aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt attttctata
      721 agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa agtaatctga
      781 taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt ctaattcgtt
      841 ggcggcatct tttgcttcgc cttttgtctt aaatcctgat tttcttatct tcgtgtattt
      901 tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt tgactaatct
      961 tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag aagcctactg
     1021 tataggtttg ttttttcata gaacacgctt gctttggacg gtgggcgtgt ttttatatca
     1081 attagttatg aagagcaatt gaaactgtct ctgttccaaa gtaacctgtt tgtgcttgca
     1141 atacattatt ttgtgcattt gcttgggctt cagagatatc aaaaactatt ttaccagttt
     1201 gttgcatatc aggatttaag ctttccataa agaaagagtt agtgatatta ccattttcat
     1261 cttgatttgc cgacatagat gcagccgaat cagcttcgaa agtttttcca tctgctttta
     1321 atttgaagaa tgagctatca acagtcacag ccttatcacc tgcatttttt acagataagt
     1381 caactactaa gaaagtgtct tttgcgttag taggaagtac agatgggcca acttgtttta
     1441 ctacttcaac ggagtttact ttgtattcca ttttccctac agcaacgtca tcaccgattt
     1501 tataagtctt ttcttctttt gctgtagttt cttttgtagt ttctttatta gtacttgttg
     1561 atgttgcagt agctgaatct ttgttttctc cgccacttag agcaccacca ataccaaaaa
     1621 acaaaagtaa tattaatacc caaaaccaca cttttttgta aaaaggttta ctaactttat
     1681 acattttccc atcttgaccc ataacttttt ttgccattta aatattcctc atttcttgtt
     1741 ataatatatt tgtgatctca gaaatgaggt atgagtccgt gttgcagcac gggctttttt
     1801 tactgtgcat aagagtattt tttcttgaaa taagactggc aaacattaaa acattctgtt
     1861 cttaacttat tattgataga atagaactcc atgaaatttt ctaatttgaa ctgagattca
     1921 tcagtcagtt cgttctcaat aaagatattt agtaaaatca taattgcgat tctatcagct
     1981 tcagtttcga attttgaatg aaaagttgta gagttatcgt acagtactga atattcaaaa
     2041 tgtgaagcaa tgaaatgacc gagctcgtgg gctaaatgaa aagcttcaga actgtcttcg
     2101 tgtagttttt cattcagaaa tactattctt ggttttggat aataaaaacc tggttcttcc
     2161 atttccatat agattaactt taaattatac tcactcagca tttctttcaa ctttaaatac
     2221 atacaaccca tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt
     2281 tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta tcgtcttctg
     2341 ataaatccac atgtttaggg gtttcttttt tatctcttcc tagaagatag tctatagaaa
     2401 caccaaaata atctgatact tttgccaaac cttcggagtt aggtgatact gatttccatt
     2461 tactgaaata tccatttgaa tatcccaatc ttttttctaa ttcccttacg gatattccgt
     2521 tttgctttgt taattctttt attttttcgt atggattcat tgatatgtca acctttctag
     2581 actgacaaga aaaaatatag aataaaactc tatttatctg ttgacaaatt agagttaaac
     2641 ctctatactt gttcttgtaa acaaatttaa caactaaaaa gacaacaaaa aacacttttg
     2701 attattaaac gccaaccgcc aaaaaagctt ttaaaatcaa tgtttatatg tcttatttaa
     2761 ctatgtacaa agtatagaat aaaactctat gctttgtcaa ttgaatttag aaaaaagttg
     2821 ttaaatttgt ttacgaatat aaaagaaagg aatgaaaaaa tgaacacacc gcaaattttt
     2881 aatttcgaac aaaacgaagt tcggacgttt ctagaaaatg acattccgta tttcgtagca
     2941 aatgatgtcg ctaaaacatt aggatacaaa aacccgagcg atgctactaa taaacattgt
     3001 aaaaaagccg taaaaacatg gggtagcgat tcgctaggtc gtcgccaatc tttcaaagtt
     3061 attccagaat cagatgttta ccgcttgatt atcaaatcga acttaccaag cgctgaaaaa
     3121 tttgaggctt gggtaatgga agaagtcctt ccaacaatca gaaaaacagg tagctattca
     3181 aacgtacctc aaagttttgc acaagcattg cgtttagcag cagatttaga agaaaagaac
     3241 caattactcg aacaacaaat tgccgagtac gaaccaaaga ttagctactt agatacgatt
     3301 ctttcatcga cagatacggt agcgacttct caaattgcag ctgattacgg aatgtcggca
     3361 attgctctaa acaaattgct taacgagtta ggtgttcaac ataaagttag cggacaatgg
     3421 atactttacc gaaaacatat gaaccaagga tacacaaaat cgcacacaag tgagattccg
     3481 aaagccgatg gcggcactaa agttgtaatg aataccaaat ggacacagaa agggcgagtg
     3541 tttatttaca acttattaat cgcagagggc tattaccctc aaatggattt agaataaatt
     3601 ggttagaaag gagttttagt atgactgata ttgcagaaat cactcaacga gatagagaaa
     3661 aaatcaaagc atatgtcgaa agttcgaagt tcttaactta caccatgctt gctgaaagat
     3721 ttggaattag taaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag
     3781 caaacagaat tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagcaaa
     3841 tgcaatatct agaagcgaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg
     3901 attatgagga gttgaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtac
     3961 tagaacggat taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat
     4021 atagaaatat tatcgatatc gaaaaaaaca aaaatggatt tgtctattat ccagttgaag
     4081 gaagagacac atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt
     4141 cggaaatctt aaggaggtaa gcggatggga aaatttaata gagcattagt gttcagcgca
     4201 cccctaatca tctacgcttt aggactttgg ggaagcagac aagcattaat cggcacgatc
     4261 gtttacatgg tttggatttt tatggggctt gatgaagctg agtacagagc aaaaaaagcc
     4321 agtcgggagg gactgactag atgagaaaca aaaaaatatc tctaacgcta ttaactatca
     4381 tcacgattgt cgtatcgcaa ctattctttt taactatatt aaacgacata tcgatgtcgg
     4441 accgattgtt ggttcaatca gctttatttc tgacctttga aaaaatgggt aattacgaaa
     4501 gatagaattt gagaaattgt ttcttgggca aataacgatg ttacccaaat tacaagacta
     4561 aaaattcttc ctggaaaagt tgatagatta atgccgaacc aactaagtat ttcgctgggt
     4621 aagagaaaaa atttcttaag agcaatcaac ggatttaaaa accagaattt tcgataatga
     4681 atttcgttgt ttttgtcaaa aatttgagaa gcgataattc taaaattttc ttcaagaata
     4741 tcatctgatt gattcaagga aagcgttaca aggctataag gcagaacttt gttaattctg
     4801 ggcatttcct taaccattat ttctctaata ggcgtcacat tattttgaaa gtaaggatca
     4861 gatttatagc taccactatg gtcatttaat cgtttgaggt catttatcac tttctcaatt
     4921 gtagtaagta aatagttaat cgttgttatt tcagacactg cattaacaat ttgcttaata
     4981 taaattattg cataaacaac tagaagcaca tacaaaatgt agttcattga aaacaccacc
     5041 atttttttac ctaaattata ccaaaaggag agaagaaaaa atgcaagaat tagtaatttt
     5101 gaaaaataaa gaagctgtga ctacgagctt acaagtggca gaaagctttg agaaaaaaca
     5161 tcaacatgtt ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat
     5221 gtttgtagaa ggtaatgaac cagattcata cggcagaaat cgacgagttt ttttcattag
     5281 tagagatggt tttttcttgt tggctatggg ttttacagga aagaaagcta tttacttcaa
     5341 acaaaaatac attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc
     5401 tcaaacaatt gaagacatga tgatctacca actagaagaa atgaaagatg ttaaaaaaga
     5461 tgtttccatg cttaaaaata ctatgcgaat tagcggacaa caagagtttg aaattaagca
     5521 aaaaggaaat atgaaagtta tggaagttct aggaggcaaa gaaagccgag cttatgaaga
     5581 aatcagtaaa aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc
     5641 aagatatggc gagttacctc gtaaaagatt cgatgatgct gtttcattta ttgaaatgtg
     5701 gttaccagaa actgcgatcc gcatggaaat cgatcaactg aacagacaac aaagactttt
     5761 cggtgatgaa aatgaataga gctgaagcgc taagaatagg gacggtaatt gctaatcgct
     5821 ggtggagaca caataaacca agcatcctaa gccaacaaca tattgataag caaaaagctt
     5881 ggcaacaaat aaaaaagtga ctcagccgtc caaagcaatg agtcacaaac caaaaattat
     5941 acctaaggag attttatcac atgaataaga aaattgaaaa tcttatcgaa gaattgaaac
     6001 gtgaatgcca aaagcaaggt gtttctatta tttgtacagc ccaaaaagaa ggggaactta
     6061 aaagtctcgt ttatggtgag acaacagaaa ttttactttg tcttgctatg caagaagaac
     6121 atttagatga aaatctccca ttgtcagcac atattatgcg tagaattgct gtggatgctt
     6181 acgaacaagc gaaaaatgaa gaagaaaatc aaccttcaaa tcatactttt gttattaata
     6241 acaaggaaga tttagctgat gtgatgactc gtattctcaa gggggaattt caatgatgaa
     6301 tgaaaaaaca attgatctac tcattatttt atttttcacg ttagtgattg tagcgaaaag
     6361 ttatgtaatc tttggcttgt tattcgcaat taccatgctt gtgctttgcg tgttagcaag
     6421 cagaaaagag gttaaacatg aacgagttag caaatcttga caactattta actgatcctg
     6481 attatgctaa accaccatat gaagcgccaa ttgatgagga ggatgaagat gagtaaatct
     6541 accttagaaa tgagccatca agaatggctc gaagaccgca aaagaggtat tggtggctca
     6601 gatgttgcaa ccgtacttgg attaaacaaa tacaaatctc cttatcaatt gtggcttgag
     6661 aaaacgggtc aaattgaatt gaaagattta gaaagcgaac cagcttattg gggtaatgtt
     6721 ttagaagaag tggttgctaa agagtttcag gaacgtacag gcaaaaaagt acgcagaaga
     6781 aaccaagtat ttgaacatcc attacatcca tttttaagag caaatattga tcgggaagta
     6841 gtgggagaaa atgccattct ggaatgtaaa acagccaatc aatttctcgg aaaagagtgg
     6901 gaaggtgaag aggtaccgct tagctatctc tgtcaagttc agcattacat gaacgtttta
     6961 aacaaagact attgttatat cgctgtcttg atcggtggcc aaaaattcat ctggaagcgg
     7021 attgaacgag atcaagagct gatcgataca atcactgaac aattagtaga gttttgggaa
     7081 acgaacgttc ttggaggtat cgagcctgta attgacggaa gtgaagcgac tgctgacttc
     7141 ttaaaagaaa aatatgcaga tgtagaagaa aatcaaacag ctctaccatc acgttttgat
     7201 gaacttatcg agcaaaaaag agaactcaag cggacgaaaa aagaaattga atcatctatt
     7261 cgtcaagtag acaacgagat catcagtgag ctaggtaaac gtgaagccag tatcggtatc
     7321 acacaaagaa acattatcag ctggaaactt gttcgtacaa gacggatgaa cacgaagaaa
     7381 ctagcagaga aatatccaga tgtcgcaaat gatgaagaga tttataacgt tactgaatca
     7441 agaaggctaa ccgaaaagga gatcaaataa tatggcaaca aatgaatcgt taaaaaatca
     7501 attggcagaa aagccacaga aacaagttgc accaggacag ttagggctta aagctctaat
     7561 gaatacacca acaatgagaa agaaatttga agaagtactt catgacaatg ctaatgcttt
     7621 tatgtcgaat gttatgactc ttgtatctaa tgacagttat cttgcagata gtgaaccgat
     7681 gtctatcatg agtggtgcgt taactgctgc aacattaaat cttgggctag ataagaattt
     7741 aggttatgca tatttagttc cattcaatag taaaaacaag caaacaggaa aatgggaaaa
     7801 gaaagctcaa ttcatgcttg gctataaagg atatatccaa ttagcccaac gatcaggtaa
     7861 atacaaagca ttaaatgtga tcgaagttta cgaaggagaa ctaaaaagct ggaaccgact
     7921 gacagaagag tttgagtttg atccaaatgg tagaacgtct gatgaagtca ttggatatgt
     7981 tggctatttc gagttactga atggattcaa aaaaactgtc tattggacca aacaagaaat
     8041 cgaagctcat cggattgcta acaataaaga tcgcgataag acaaagttaa gtggtgtgtg
     8101 ggcatctgat tacaatgcaa tggcacgaaa aactgttttg agaaatcttc tttctaaatg
     8161 gggaatcttg tccatcgaaa tgcaagaagc caccacatcg gatgagagag tccaaagggt
     8221 tcaagaagac ggcagcatta ttgctgaaac agaagttgaa gaagatattc ctgaaagaaa
     8281 agaagcagag gttatatctg aagaaaatga agatgtacaa actggattat ttgatgcatc
     8341 taatccgccg ttaaacaaat aatgagggag ctttctccct tgcttttcta gaggagaaat
     8401 acgaatgaac acaggatatg tcaaattaca tcgaaaagtg atgaattcat tcgtttggac
     8461 caatccttat atgtataaat tatggagctt gtgtttgatg aaagcgagtc atgagaatcg
     8521 caagatactt tttaatggaa aagagataca agtgaacagc ggagaattcg tcacagggcg
     8581 cgatgctatc acatctgaga tgaacgaagg cgtgaaacgt gaacatcaag tgaacagcgg
     8641 ttctgtatgg agatggctaa aacaatttga aaaaaacgga atgttgaaca tcaaatcaac
     8701 tacgaaatac agcgttatat caataaaaaa ctggtctttg taccaaagca gtgaacaaca
     8761 aatgaacatc aaacgcacaa caagtgaaca acaagtgcac acaaacaaga atgaaaagaa
     8821 ttataagaat gaaaagaata ataaaacatc atcgtcgcaa ccacgcaaaa agcgtgttta
     8881 cgacaccgac tcagtttact acattctcgc ggaggagtta ttcaaacaga tttgtcagaa
     8941 tcaggaaatc aaaaagccga atctgcaaag ttgggcagat gacattcgga aaatgatcga
     9001 gatcgacaag cggaccgaaa accaggtacg gggaatgatt gaatggagtc agcacaatgt
     9061 gttttgggca tcgaatattt tatctgctaa aaaattgcga gaaaaatacg acacaatggc
     9121 agcgcaagca aatcgtgatt ataaaacaaa acaaactaaa acgcttgaat acgagaaatt
     9181 tagcacagat gagttgccta tttgagagga ggcgtaggca tggaaactgt tggggaaatc
     9241 atggaaaagc tgatacagaa agtgcttgtc caacgtggcg aatgtcctga atgcggacag
     9301 cctttgtatg gatggcgtac gaagaaccct gatggctcag aacgttgtaa gccaacctgt
     9361 atgcaatgtg ggtataaagc attgcgagtt caggaagact tacaaacaga acgaatttac
     9421 aacgagagcc tgaaagcaag agcaatcaat tttttcaaag gtggttctgt tgtgcctaat
     9481 caagcgttgt ttgattgcac attgcagaat tatcaaattg tcgatcaaga aacaagacaa
     9541 gcggttgaag taaccaaacg ctttgttaat tcagtcttgt taggaaatcc aagtcacctt
     9601 gttttaactg gaaaacaagg aacaggtaaa agtcacctag cgatggcagc ggcttgggaa
     9661 gtcttgaagc gatcaaacta cgataaaaaa atcttattta tcgggttaca ggaaatgctg
     9721 gatcaaatca aattttcgta caacaatcct gaactcagaa aaacgattga gggatcgttg
     9781 attgcagata tcaaaacagc ggatttagtc atcattgatg atatcggttc agaactagga
     9841 aaagatgcat cagatagtcg agcgtttggc ataaacacgc taaattcgtt cttggatgca
     9901 cgacagaacc tagcaacgat tatcacaacg aacttgcttg gtgaagaact gaaaaaagct
     9961 tacggtacga gaacaatatc aagaatgttt gtcaactctg atggatttac gatggtattt
    10021 tctcaaacag cagacaagcg cataaaacca gtcaaaggag cgatcgcatg aatttgacta
    10081 aacaagaaaa aagaatctgt cgtcagataa tgttgcaaat tataggtgat cgtgacatgg
    10141 acgaattatc tcaacaagag agtcgaatgg ttcggaagat aagtaagcta atcggtgcag
    10201 atacgatgga tcgatccaag ccattaccca aactgaattt tgaatcattt acttttgaga
    10261 aatatagcca tctgagagag gtaggctata gtgtaaatgc tattcgaaaa gcacttgggg
    10321 taggaagtag tgtatgggca aaatggcgga aagaaaatgt aaatgaggta aatcaaaatg
    10381 attaagctaa taggcaaaga gacgctcaag gtttactatg tagcggaaac aaaggcagaa
    10441 gtcagccgtt ggctattgaa aatgtttaat aagactagag ccaatgcttc agtaagtcag
    10501 aaagcaagtg tatcagaaac aggcatgcct gaagcaatgt ggatttcaag gaggaactga
    10561 gatggcaatg ataccgaagt ttagagcgtg gtacacacca tttaaaggta aaacaattgg
    10621 acaagaaatg aaatatgggc aagcaggaag gttgatcact catgctgaaa tggctccaga
    10681 taaatatgtg cttatgcaat ccacaggact gaaagacaag aacggtgtgg ggatatttga
    10741 aggggatgta gttcttgtca atgtaagtaa tggttttgat catttagtta atgaaaaaac
    10801 ggttgtacaa gaatccgagt ttcattctgg attagtttgt aaatcattaa ttagtggaat
    10861 ggaatataga gtattcaaac acaaaaagat ggattatgaa tatgaagtca tcggaaatat
    10921 ttacgagaat tcagagttat tggaggtaga gtgattatct tagctactga ctacatcgat
    10981 tctttaaaag atgaagatgg gaatgtccca gcattgctaa attgtggaat taaaagtaag
    11041 aatagaatca ttttgttacg gatatcggct gatttagcaa aatatcttta tctgagcaag
    11101 acagcagtat attacactgc ggctacacgt tatcagtaca agggaaaaac aatcagtcag
    11161 gattattatg atcgtttaat cagtcctgac atgcatggaa aatcagaatc agcgatcaag
    11221 ttatttggcg caatagagat atatgccgat gattttccaa atttatggtt gaaggaggaa
    11281 cagtaatgaa atacgaaata ccactaagcg aagcagatgt tcagtcaatt attaacggtc
    11341 gggaggttaa taaaaaactt tcagatggta ctgaaatagt tatcagacaa agttatttga
    11401 aagatatggc agctcctgta ttaattgatc gttttaacgt gactgattct gcggtagaga
    11461 accacttaaa agaatttcga tcaagtatag acgacacttt cagattaggg agttgattga
    11521 caatgaacaa cagacatcgc agaataacaa aactaagaaa acgggaactg aatgtactaa
    11581 agacaaagtt tgaaaaagaa tatggaattt cagcagaaga aacatataaa gtggcaagtc
    11641 agtgtgttgc tgatgcaagt gatgctattc gtaagtttgg aatttcgata ctagatatta
    11701 aatgggagga tacagaatga agatttatgt agtaaagttt ggaaataaat tttacagaag
    11761 tgatgaacgt tctatagggg ctaacacatt atccattgta gacatactcc aaagtgcaaa
    11821 atggtttgat aatctcgaag aagctaacca agtttcacga cgacttggtg gattaacgca
    11881 agtatatgaa ctgatcacta tcgatcatga ggaggtagag taatgaaact aaaagacgga
    11941 ttttacgcta gcagtcacgg tatcggcggt ttaatgctag atatgccgac aaagaatcct
    12001 aaaacacgta agaaaccaaa agtcaaagtc ggtgacatgg ttcgctgcga agcagaggag
    12061 ttcgtttatc ccttcagagg atacgttaaa aagatactgt caaactcagc aatcattcgt
    12121 attgaaaaca cgatgaaatg tgacaagtgg ttagcgaaaa gcaaagagaa tctagcagtg
    12181 gtgagattgg tggatatgga agttataaaa tcttaatttt gcttttttac aactatccta
    12241 caacaatgag cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga
    12301 atatagatat taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt
    12361 tagaacgttt ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt
    12421 tgaaattcac aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt
    12481 cggttatcga agcattaaca cggctaagta gaatccattt ccaggtgctt tattatagtt
    12541 attgttttcc taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata
    12601 gaactatcga gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag
    12661 gagaactcat ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt
    12721 tccgacataa gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa
    12781 gaaatatgag acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc
    12841 tgtgtttctt tcagcatatc tatcagctag atgtagagta gggcaacctc gagaaccaaa
    12901 agtgacagca tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga
    12961 aagaatgctg atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt
    13021 tgttcgaggc tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa
    13081 gaagatattc tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa
    13141 taatactagc gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc
    13201 atcgttagaa ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta
    13261 ccaatttggt ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta
    13321 ttatgatagt gtcgaaagat taggaaacag gatcgacaaa ataaaatgta agggaggaaa
    13381 tctccctcat cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat
    13441 gtgaatttca actccttcta aattgttctt attatctatc atccgttgct gtctattaat
    13501 ttatgtattg gaggaaaaac gaatggataa agaaatcaaa gcaactgtca aattagattt
    13561 gactgaacta aaagaactgc tcaacaaggc tagtgaccaa gtcgaacagt tacaagaagc
    13621 tttagatgaa attgctaatt ttaaaatcca agtttcttag cagtatattt tatagcagct
    13681 gcactcatga aatcagacca agtatcaaaa tttgtattat cagatacgaa tgcatccatt
    13741 tgatcatcag gaatcgcggc aaacgattct tcagagctgc agtcaaatcc gctaacctca
    13801 aagaattcct caatggagct gaatttggta ttagctgaaa caaaatcatt tgtaaataac
    13861 tcggacattg gaacgctatg ttcaccgtcc atttcttggg cagcttttgc cattttgtta
    13921 agttttttac ttaaatcatc aaacccgtta ctcatgttat caccacctat aatttatttc
    13981 agcggaccac tcgctgataa ataaaattat acgcttagta tttattttca caatattaat
    14041 ttatcgtaat aacttttgag taaatagaac aaaaaacctg cactagtttc cgctagtaca
    14101 ggcagtgact atatcggttg ataatctagc atatattaaa aataattgca agaaaagaaa
    14161 tttatagtgg tttataccaa attatcaaag caatgttatt ttgttgctgt ctattgtttt
    14221 ttaattattc acacgataac taaaggtggg tgaagagaaa tgattccatt aataatttca
    14281 atttttgcgc tctgtcttaa tgtctatatg attggattta aaaatgggca aaataaaaaa
    14341 tagtagcagc caagaataat tttatagtgt cgagtgtcac tgtggcggaa atagaagaca
    14401 caacggtaaa tgacgagtag cctcgtgaga gcctagtaag ttctcgtgtg tggtgcgatt
    14461 ccactccagt gactttgggt ttgcggtaca agatcccgaa agtaaaaccg ctagcaaccg
    14521 agggttggaa atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga
    14581 atcggtgtag gttgctatta catagttggt taggttagat tgagttttgg gatttggtac
    14641 aaatgaatcg tcaaatgact caagcacagg atcggaaacg tccctgcctg tgcattacat
    14701 attagatcac tctttgagtg gtctttttat tttgaaaaaa ggaggtaaca acaatgtata
    14761 gaccacaata cttagaacag aagcatgaag taatcactgt gcaaaacggt aacggtgaga
    14821 tagtacgaaa gtatagaaga ccaataaaga gcgatacata taaacggaag gaaagcaatg
    14881 aagttattcc attgtatggc aaaagaatag ctaagtatta aataagattg cgaaaggaga
    14941 tgggacatga ccgaggaatt ctatagatgg ttattacagt tgataagaga agatcgtttg
    15001 gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat gaaacgagat
    15061 cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga gaacgttcat
    15121 catataaagg aagtcaagga tagacctgac ttagctttag atttagataa tcttatttgt
    15181 ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt agataaacaa
    15241 gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta agtgcatgat
    15301 catcaatgac aatggcagag agtatgatac agagaagatt gaagagtatt catcttatac
    15361 acaaggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg atctgttatc
    15421 agataactgt tgcagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa
    15481 taacgttgaa agaataaaaa atatttttgg atatagcatt gaagagatta attattacat
    15541 tgacttcgct gaagctttca ttccgatggt gagataaccc cccttaaaat aaatcgcaaa
    15601 ttttttgggg gtgatgaaac ggagggggct gtcaggaaaa gagatttttt cgaactttat
    15661 catgaaagga gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa
    15721 ttacgccgct ctttaataag ccaattggaa agtcagaaaa caaatattga gccattctta
    15781 gataatgttg atcgttatat cagtttatgg gaaacggcga tatcactgga agaagatata
    15841 tccgagaacg gcattagact ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt
    15901 tctgtcaaca aacaaatggg attgatgttg gataaacttg ccattactcc tgaattggta
    15961 ggtgaagcaa atgaatcaat tcctgagtta taagcatatt gaaaattggt tcaaagctat
    16021 agaagaaggc actatcaagg tatgcaaaga gcaattattg ctaaaaaatt atctagaaga
    16081 aagagtcttt actagagaag atatttactt cgataagcag atggtagagg attcaatcaa
    16141 tataccagca caatactttc cattcgaatt aattccgtgg gaaaaatttc tacaatgttt
    16201 tatttatggt gttcgatgga aaaaagataa aacactagtg ttcaatagat atctttcatt
    16261 aatgggacgt ggtaatggta aaactggttt tgcttcttgg aacaacttct ttctactaac
    16321 cgctaaacac ggtattaaaa attatgatat tgatatctat gccaataatg aaagccaagc
    16381 aaagactagt tttgatgatg tatttaaagt aattaaagat catcctgatt tagataaaaa
    16441 agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca aacttcgtta
    16501 taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc gctttgatga
    16561 aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg gtggtaaaat
    16621 tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg gtccgcttga
    16681 tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg acaaggatgg
    16741 agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata atgaagttga
    16801 tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcag atttaaaacg
    16861 gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac ttacgttcat
    16921 gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt catatgatga
    16981 tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga taggaacagt
    17041 cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat acgataaaga
    17101 tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac aaaacatcaa
    17161 acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc atggaaaaaa
    17221 tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat attatattaa
    17281 aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag aagaagcagg
    17341 ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa aagatctggt
    17401 tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta tgcgttggta
    17461 ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg aaaaaataga
    17521 acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga attttttaga
    17581 tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa atacaggaac
    17641 tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg atttttcaaa
    17701 aggcggtagg atacttcaca aaaaaagcaa cggttccttt agaagaatac ttttgtaaat
    17761 tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa
    17821 atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc
    17881 tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa tttagaaaaa
    17941 aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg tctaataatt
    18001 ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg aaagatgatg
    18061 tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta gaaagtgatg
    18121 ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg tattatcaag
    18181 cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac gctcgtagat
    18241 acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat caaatcaata
    18301 aaatgatgac atcacaattt aaggatttta tggaagctga taatgcaggt gcggtatttc
    18361 aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc aattcaagag
    18421 atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt cacgttccga
    18481 aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct tttttaatgt
    18541 tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt ctctatgaag
    18601 tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc aagattacta
    18661 gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta tttacaatca
    18721 acgaaggaag agaacgcgtt ggaaatgatc gctctgataa ggcgatggca gatgaaatat
    18781 ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac gacaatgaaa
    18841 acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca gggatttatt
    18901 ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat tttggatagt
    18961 ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga cgtatttcaa
    19021 gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt tgtgattaac
    19081 ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat aaaaatctac
    19141 aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac
    19201 ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc ttcatataaa
    19261 acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga atcgtttatg
    19321 gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc
    19381 tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa
    19441 aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt tggacaagga
    19501 gatgcaaaat aatgacaatt aaaaatttaa aaggtgtaac agctgcaagc gaccaattga
    19561 tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta agcttatcta
    19621 aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat caattagtat
    19681 taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat aacgaagtag
    19741 tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta tttgaacgtg
    19801 tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt gttaacacaa
    19861 ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt
    19921 gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg aagcagttca
    19981 agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca gtatggttag
    20041 atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa caagcaattg
    20101 ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc aaacaaacta
    20161 gcggagaata tgctgaaaaa aaagcagaac ctattacagc tttagatgct gtaactatgg
    20221 gcggtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat ccgatttatc
    20281 gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac tggtctaaag
    20341 tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta ttgccagtac
    20401 cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt ggggtagcct
    20461 cagattactt catgggtgta ggatctacac taaaaattga agcttcagat gaataccatt
    20521 ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa cctaaacgta
    20581 acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca actacaaaac
    20641 caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat attctttgtc
    20701 agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat ctgaagaaac
    20761 taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt cctatttttt
    20821 ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac gacaaagagt
    20881 atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat cattcgcgtg
    20941 aggacgaccg atatttctat atcgattcgg atgtcatttt caataaaaga attaatttgc
    21001 gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt tatctaagtc
    21061 ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg gcaaacattg
    21121 taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc gcacagtggg
    21181 ttattaaccg tcctaaagcg aattattgga aaaaggttta tctggattct aatcggctat
    21241 atcgctacct tagagggcaa aaaacaaata tacaaatctg gacagccgag atgtgggcac
    21301 agctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa ttagactttt
    21361 gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac aatgctggag
    21421 taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg
    21481 atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta
    21541 aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga
    21601 taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa
    21661 tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga
    21721 atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga
    21781 acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat
    21841 tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt
    21901 taaagatttt gacgcaaagt accaaaagaa atgaactagg aaaaaagatt ggtgtagaag
    21961 atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt gatataacag
    22021 ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg
    22081 ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta
    22141 tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg
    22201 gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact
    22261 tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta
    22321 cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca
    22381 aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta
    22441 acaggagact cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga
    22501 atggatccca atcatttaaa actagagaac caagatcgct atatcgatca attggtattt
    22561 acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat
    22621 aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact
    22681 gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa
    22741 aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc
    22801 acttaagact caatacatta atttgggttt catcattaga cctacaagaa aatttgagta
    22861 tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat
    22921 gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga
    22981 taaattaaat aaataggggg aacaacaatg gctaaaacaa aaactgtagt aacaacgttc
    23041 gataacgtaa gtatcaaacg aattgctttt aattttaaga acgcaggaaa tgcaatcgca
    23101 acgaattgta acggacaatt agatggcgaa acagaaatgc aaacggtggt taaaaaatgt
    23161 ggagcgacag aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac aattactgca
    23221 catgtaccga tggaagttta tcgacgtttc aatgggttga aacaagatgg acgtattaaa
    23281 ccaggcattt actcttacgg tcctgattcc gtaggcgaag atttctcact tgctgcagag
    23341 atcgtggatg acttcgaaga aaatagaaag ttagttggta tgttagcatg cacttcgaat
    23401 acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa
    23461 acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt
    23521 gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact
    23581 tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa
    23641 cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact
    23701 gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat
    23761 gtcgatggga aaaggctcta tggacattac agttattcaa ggtgcaaaag ccgtatatgt
    23821 agcttatcgt caagcgaata tggttgatta catttcattc gatgaattta tcgacaaatg
    23881 ggattttgat atggaggttg cagtagctgt atacagtact atgatgttca aacaagcacg
    23941 agatgcctat caaaaagaat tcgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa
    24001 atgccaaatc tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt
    24061 tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct
    24121 tacaacaaga gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac
    24181 taaagaaagg aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa catttatagc
    24241 taacgatgac ggattgaaat ctacgttaaa agaaatcagc gctgaattaa ctaaaaatag
    24301 agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt
    24361 aggaagtaaa ttatcttctt tagagaagca gtatgaatta caaagtcaaa aagttgaagt
    24421 aactagtcaa cgtttagcca atgcgaaaaa atattatgga gaaaattcca ccgaagttca
    24481 gaaacttgag agagaactga ttaatcaaca aacagcgcaa caaaggttat ctaatgatat
    24541 cgataaaacg agtaaagcat tggctcaatc taaaggtgaa tttaaaacct atgagtcaac
    24601 tatgaaggat ttagataatg agcaacaaca cctaaaatca agtgcatcat tagtagaatc
    24661 cgaatacaaa aaatggcaag caactgctgg tcagtctgct accgaatctg aaaaattagc
    24721 aaaagcacaa gaatatgtcg gcaaacagag tgatattgct gaacaaaaaa tcgaagtgct
    24781 aaaaaaacaa ttagatgcca cacaaaaaga atttggagaa acatctaccg aagctttaga
    24841 tatgaaaact aagcttaatg atgcggaaag agaattcgaa gaattaagta atgctgctaa
    24901 aaacgttgat acatccacag tagacgatat cggcaaaaaa cttgatatgg gcaatttaat
    24961 ggaagctact gatcacttgt cagtgatcgg agataagctg attgatgtag gcagtaagtc
    25021 tattgaagca gctggaaaag cacaagctat gcaggcccaa tttaaacaag tctttggctc
    25081 tttagaaggg gaagcgcagg acgccgttga gggaatggct gaaaaatttg gaatgttacc
    25141 aaatacgatc aagcctgttt ttacgcaata tacgtcaatg tttaaaggac ttggatatga
    25201 tacaaaagaa gctatggagt tagctggtga cagtactcag ttagcagcag atgcagcagc
    25261 tttttacgat aagtctatgg atgatgctag tgaatctctt aattcattca taaaagggaa
    25321 ctacgaaggt ggagaacaaa taggtctatt tgctaatgat actcaaatgg ctgcttatgc
    25381 tgttaagcat aacttgatac cagcgactga aggagcaaaa aaagccagtg aagagtcatt
    25441 gttagccgtt gaaaaagcac aatctaagta tgctgatgct attaagaaac atggcgaagg
    25501 atctttagaa gcaagagagg ctgctttaaa acttaaagat gcgcaagata aaataaatga
    25561 agaattaggc ccacaaactc aaaaatggtc tgatttagat gaggctacca aacaagcagt
    25621 tcgagttcaa tatgctgaag atatgcaaaa attagccggt gctacaggtc aggctagtag
    25681 agagtctgat ggtttagaaa accaaatgac aagagcaaag caagcattag aagattttta
    25741 tgcctcgttg ggtgaagata tattgcctgt gtttatcaaa gggttacaag caggcgcgaa
    25801 agctttgcaa ggattagccg aatggtggag taaacttgat ggaccaatga aaaatttcat
    25861 tttagctctt ggaggaattc tagcattatt aagcacatta gctcctgtta taaccgcagt
    25921 tgttacgata gttggcacat ttggttctac agttttgctg ccaataatag gaattatagc
    25981 gggcgttgca gctgtaatag cgattgtaat cacagcgttt cagaactggg gtgcaattac
    26041 tgactggttt aagggaatat ggagtaaatt cactgattgg ttgggtggta cttgggaaag
    26101 cataaaagaa ggtgcctcat cagtttggga tggagttaaa gaaacctggt ctggatttgt
    26161 agattgggtt caagaaattt ggcaaggagt ttctgattgg tttggagagt tatggagcgg
    26221 attagttgaa ggagcttcca acatctggca aggagtccaa gagacttggc aagcattcgt
    26281 tgattgggtt tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga
    26341 cattgtagga attgttcaaa taccatggac attaataacg tcattgattc aagctggtat
    26401 taatattatc gtgggtatct ttgatgtagc tggacagtta ttaggcgcag cttggcaagc
    26461 tgtttggaca cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat
    26521 aagcatcgct tggaatggaa ttgtaactac attccatact atatttgatc cagtagtggc
    26581 atggtggaat ggtatatgga catctattag tactacggct tcaaatattt ggaactcaat
    26641 tagtgcaaca gcttctagta tttggaatag tatcaagaat acaatcacta gcttggtaca
    26701 agcagctgct acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat
    26761 ttggaattca atcagctcta cagcatcaaa tatctggagt agcgtgacta gtagtataag
    26821 caatgctata aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg
    26881 gatcagcgga atttggaacg gtatcaaaaa cactgctttg aatctttgga atggaattac
    26941 aagcactatt agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa
    27001 tctaacaggt attgtttccg acatattcaa taatgttaaa agtacgattg ctaacatttg
    27061 ggaaggtatt aaaaagactg ttagcgctcc gattgattgg attagagata aaatcagtgg
    27121 catctttgat aatctgaata tttcgatacc acatattccg ttaccacatt ttaaattgag
    27181 tgggaaattc aatccattga agggaaaaat cccaacgttg ggtgttgatt ggtatgcgaa
    27241 aggtagtgtg tttaattctc cgaatattat cggtatcggt gaagcaggac ctgaagtagc
    27301 tttacctttg aaaagatctg tgctgcaaga aattggggat cgtatcttga gtagcacctc
    27361 agtttcatct agggcacaaa cggttcaacc tgtgaacaat tacgaattca atttcacaat
    27421 tgatggtaac gcagatgagg ttactatgaa gcagacaact caacaaatca ttgacagcat
    27481 tacaaaagtt caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg
    27541 ttttcttagt attacaaagg atgtgaaaaa atgactgatt gtatacattc tatagtcgat
    27601 ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca
    27661 aaaagacaga gagttgaaac ttctgtttta ggacggctag gtggcttagt acaagattat
    27721 tcgtttgaag atatgtcgtt tactttgcac tataactatt tagaggatgt ggaagaccat
    27781 caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaattatgc aaagaaatta
    27841 gaattctctg atgatcccaa cgtctattat gttatccaga ctatcgatat tggggatgca
    27901 gaaaacgata ttgttgaatg gggagagttc gatgtaaata tcactgcgaa accattcgca
    27961 agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat
    28021 aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccaa
    28081 ttcatcttaa atgattatgt gtttagtttt gaaggcttag tagtaggaac tgacatagtc
    28141 attgatagtg atctaatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg
    28201 tccaacaaaa tgaagaccat gcaatatccg acattgcaag tggatatcaa tcattttaat
    28261 tgcactggtt tgagcaaaat acaaatttat cgtaatgggt taaggtaggt gaaatagatg
    28321 atcgataatt taataactat ttacgataaa aatgacgcga ataatttagc tgaacattta
    28381 tatgatacgc aaggtttagg cgctttgtca gactggttaa cagccactgt tagcaataaa
    28441 ctaaacggag ccgagatatt tcagggtact tatccaataa gcggaactaa tgcagatttg
    28501 attatagaag gacgtattat tcagtgctat gtagatgaaa atcgagcaaa gcaacgttta
    28561 cggatttatt atgcaaagac ttctgtaata ggaaatacga tagaagtaaa agctgaacct
    28621 attttcaatg atataagaaa atcggtgttg aataaatatg acagtggaac agaaaagatc
    28681 actgctactc aggcatggca aaacgcaaaa gctttagcga aaccagctat cccttcgcag
    28741 ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa agcgaatttt
    28801 ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg ggaatttctt
    28861 aaagataata acacattacg tcatgaaaaa aggctaggta cggatcataa aatcaaagcg
    28921 atttatacta aaaatttaac tggtcttgac ttagagatag atgctcaaag cgttttagtt
    28981 ggagtttatc cattcattag cagctcttca gaaggagaag acgagatcac tctaccagaa
    29041 gaagttattt tcacggatta cgtggatgat tatcctgctg gatatgtttc ttttgttgat
    29101 tttaaagaca aagcgactga tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa
    29161 acaaacatag ataaacaaaa accacaagtg agtggttcga ttgaattagt accattgagg
    29221 catcaaagag gctatgaaaa atttgttgat ctagaaaaag tttcgatggg tgacggagta
    29281 gatgtgtatc atccacagtt aaaagtgaat atgtcagcga gaattgtgga atatacgttt
    29341 aatgttctaa ccaattcata cgataaatta gttgtaggaa acgtcaaaac aaacttctta
    29401 gaaaacacag agaataatgt cagcaatttg attaatgatg ccattgatca attgaaaaac
    29461 ggtggcgaaa tcagtgattt aatcaatgat attgtagatc atcaaactga tatgattact
    29521 ggtcaaaatg gtggttatgt tttattagat cctaaagaag cgcctagtcg tattttgatt
    29581 atggacacac cagataagaa taccgcaagg aatgttttac aaatcaacaa tgctggtatt
    29641 ggtttttcta aaactggcat taatggaaca tatgaaacgg catggacgtt agatggcgga
    29701 ttcaatgcct cgtttattac ggctggtgaa atagtaggaa ttactattag aggtactaca
    29761 ttaattagtg atggtgctga ttatagaaca agtattgcta atggcaaaat gacttggtac
    29821 tcaaaaaaag ttaacaaaga tattatggag ctagaagcac gtgattatgt aagtgctgat
    29881 gccggtattg tatcatacac catgaaaact ggtggtggtt tcatgattag aaatccacag
    29941 ggtaacttgg tttttagtac gtgggataat ggtaataaca gaccgtttct atcttttggt
    30001 gcgcccaatt tcaggtatag caatgctagt tatgtaactt ctggcgacgg tagttcttta
    30061 agcattaatg gtagtgcggg taactcatgg gaatttaagg tagctggtag gactatgaaa
    30121 tttactagtg atggtatgct aacgttacca ggttgttttt ttggttcatg ggaagatggg
    30181 aaacttgcta ggtttgaaca atcaacggta caagtatata aagattttac tgttagaggt
    30241 actaaaaact caactgtacc aacagaacat tatggacaac gactattgaa cgcttatgaa
    30301 actccagaat attatttcgc tgattatggg gaagccgtta caggtgacga tggtaaagtt
    30361 cgtgttgata ttgaccccat gtttgctgag acagtaaatc taagtcggta tatgacacat
    30421 gtgacaccta cagaactagt tttgtgtgct gttactcatg aagatattga ccatttcatc
    30481 attgaaacta gtaagccaaa cgtattagtt agatggattt tagtggcaca ccgtctaggg
    30541 tatgaagata ttagattaaa agaggataca gcatatgata gcacagtgct tgaccaaaaa
    30601 cgtttttaaa acgaagacaa ggaggtatat aaatggctag cagtttatat aatttggctt
    30661 tagatttcag caaagaatta aactacacca aagctattat ggctcgtcag ggtgataaag
    30721 ggattacggt gacggttaaa ccatatttaa atggcttgca gatggatacg agtggcggaa
    30781 catttacttt aaaaggaaca acaccatcta accgttacgt agatagtgtt gcaactagcg
    30841 taactagtga agaagtcacg ttttctcttg atggcacatt tatgagtgaa gcaggatatt
    30901 ataaacactg ctacgtagaa tatagaaaag acaatcaaat tttaacaacg caagatatca
    30961 tttttttctc actaggagtg tctgacattt cgcaaggtca agccgatgaa tatgtttcgc
    31021 aattagaaga gttgattcga aagtataatg aaacttttga tgcttttatg gctgaaacta
    31081 aaggtagagt ggatagctta aatcaacaga ttactgattt aactggtcaa gctaaaacgc
    31141 tacaagacaa gttagatgct ctgaaagaag aaatttctaa gttaggtaac ttacaagtga
    31201 tgtacagtaa cagcattgat ttcgggggct atgattattc ggggaatccg aatttgatgg
    31261 ctaatataaa cgctgatagc ttctcgcaag gtagtggtgc cttatctgtt gtagatgatg
    31321 gtgacgaggt agtgattacg cttgatccaa accataaatt agaggtatta aaaccgaaaa
    31381 gtcaaccagc cctattaaca ggcaaaacat ataccgtgag tgtagaaatt atgttagaag
    31441 gtgatttcac tggagaccct agcaagatag gcctaagata tattaaaatg cctaactggg
    31501 tatcagagct atatacgcgt aatacattaa ctgctactaa gggtgtatgg caaaaactaa
    31561 ctggcaccgt taaaattact gctgcgagtg ataacgctga aagctggctt ataatgctac
    31621 aaaataaaga cgctaataac agcctatccg gcaaactacg tttgagacac gctaaactcg
    31681 aagaaggctc aacagctaca ccattccaac ctaacttatt agcagaacct tacaacatgt
    31741 gtcgcgaata tcctaacgaa aatattgccg atcctacagt taagttccca atcgaatctg
    31801 gcgaccacca aatatatcaa ggttacacag aagaagagct tatgataggt caaacgtata
    31861 ctatcacgct taaaggaaca aaacccgcaa gtcaaacctt tgtagcgtat aatcattgga
    31921 ctgctcgttt aggagaacta aagccggttg atgggttgac agacgtatgg tctctaacat
    31981 tcacaccaac gaatgttgtg gcgatgccta aacttttccg tgtttatcag tatccacgat
    32041 caacagtagg cgcatgccgg atcgactggc tcaagatcga aaaaggcaac actcgaaccc
    32101 cgaatattag tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag
    32161 attatgtttg ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa
    32221 tttctgaaat tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg aagattaatt
    32281 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taagggtgct caaacaaaag
    32341 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    32401 atacagctac taccgcattt tatggcgcac aaggtgaagt ctctaacaaa acgaaagtag
    32461 cggagtatgg agtaggaaca gggtatacta ctacagctgt aggagaaaca catttcgaac
    32521 gtcaatctaa cggatacgtt aaatgcttaa aagctggtaa gtattcaatt agtgcgcaaa
    32581 tacgtgtgca attgggcggg aaatatgctt catggttata tactgattta tataaagatg
    32641 gaacttctgt tgataattta gtatcttatg gggtcgacgc cttgcaaaat agatttgcag
    32701 ctagcggaaa cattgtaact gatttagaag taaacgatgt actaaatatg cgtactgaaa
    32761 tcggcgttgc aaatggaatt ttacaattta catcttgccg tactttagtc ttgactagac
    32821 tatctgatta atacaatatt tataattttg ctatcaacac gctcaaagga gggtgttttt
    32881 tattttgcaa tgaaaggagg atagttggtt gaaagacgaa gcaatacaag acgtggtaga
    32941 acgcttagtg cgtattgaaa cgaaactgga taattacgaa ttattacgcg aaaaagcgga
    33001 aagtgcaaaa gatagagcgg atcaggcata ttctattgcg cttaataatg cggaagatat
    33061 caaagaaatg aaagccaata ataaatggtc gtggggttac atgatcggtt taggcattac
    33121 gatcattggc tatttcttga ctaaattgta aaggaggtga gaagaaatga ttttacccga
    33181 taagtattat caagtcatta aatggacggt tttaacagta ttaccagctg catctgcttt
    33241 agtagccatg ttaggcaaag cctatggatg gaatggaaca gatatgacag ttctgactat
    33301 caatgcagta gccacgtttt taggcgttat cactggtgtg tcggcttata atttgaaaaa
    33361 ataggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagccta ttaacggctc
    33421 tttttttatt gccaattaat gggttcgcct atacgattaa caatgaattt aatttgggcc
    33481 taaacgaagg tagctcacaa gtagcaaata atcagtacat tttactgcat gaaacggcta
    33541 atgaaacagc aacaggacgc aatgaagcgc aatatatgca acgttcatgg actagtgctt
    33601 atactgctta tattgtggga gacggtggaa ttgtttacca agttggacaa cctggttatg
    33661 tacagtacgg tgctggttcg tatgctaatg ctaacagtcc tgtgcagatt gagttacaac
    33721 acacacataa taaagcaact tttgaaaaga actataaagc atacgttgaa ttggcgagag
    33781 attccgctaa gaaatacggt attccgctta cattggacac tccttataac caaccaggaa
    33841 tcaaatcaca tttatgggta acacaaaata tttggggcga tcatacagat ccttacggtt
    33901 atctttctga aatgggcgta agtaaagaaa aattagcata tgatttagct catggattta
    33961 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
    34021 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
    34081 tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata
    34141 ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt
    34201 atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt
    34261 tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca acgaatgatc
    34321 cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata
    34381 ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata ttgacaacta
    34441 taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac ccataaatag
    34501 tcacactcca agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt
    34561 atggctttat gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat
    34621 aaccgtccgg tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt
    34681 ctgtgttaga atttttttga agagtattat acaagctaaa gcttttcttc attgccactc
    34741 aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt tcacatagtt
    34801 atacttctgt atatttgaaa agttttactt tgatttttaa atagaaagac atttgggtta
    34861 aattgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt ccccataagt
    34921 gtgttacgct ttaaactctt ttatatttga agccattaaa aagcatacca tatttttgaa
    34981 aaaaagtgag aaaaaaggct tacaattgga gtggtagtta attagtgact tatttttgat
    35041 tttatagcac tgatactata aaatatagat atcatcatat tacacaatct taatactaac
    35101 ttaaaaaata tctcctttca caagtatggt gataaaattc gttccgggct acctttttag
    35161 gtagcctact ttaatcttta tacctttctg gatcaacgaa agtatacttt atatagtcat
    35221 aacgccgatg atcgctacgt gcgtccggca cgtcagtcat gaatcggcta cactagacta
    35281 gacagaaaaa ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgtcaa
    35341 gacgtcaacg aagaacctat tcaaaagaat tcaaacaaca aatcgtcgat ctctatctcg
    35401 ctggtaagcc tcgcgcagaa attattcgag agtat
//
LOCUS       DACCPY010000018        34514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCPY010000018 DACCPY010000000
VERSION     DACCPY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.018"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(167..2059)
                     /locus_tag="IVO69_000787"
     CDS             complement(167..2059)
                     /locus_tag="IVO69_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399586.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2056..3783)
                     /locus_tag="IVO69_000788"
     CDS             complement(2056..3783)
                     /locus_tag="IVO69_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399587.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3799..4449)
                     /locus_tag="IVO69_000789"
     CDS             complement(3799..4449)
                     /locus_tag="IVO69_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7399588.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4653..4949
                     /locus_tag="IVO69_000790"
     CDS             4653..4949
                     /locus_tag="IVO69_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7399589.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5141..5761
                     /locus_tag="IVO69_000791"
     CDS             5141..5761
                     /locus_tag="IVO69_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7399590.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5853..7019)
                     /locus_tag="IVO69_000792"
     CDS             complement(5853..7019)
                     /locus_tag="IVO69_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7399591.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7031..7384)
                     /locus_tag="IVO69_000793"
     CDS             complement(7031..7384)
                     /locus_tag="IVO69_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7399592.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7512..8534
                     /locus_tag="IVO69_000794"
     CDS             7512..8534
                     /locus_tag="IVO69_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7399593.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8973..9407)
                     /locus_tag="IVO69_000795"
     CDS             complement(8973..9407)
                     /locus_tag="IVO69_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399594.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9517..11376)
                     /locus_tag="IVO69_000796"
     CDS             complement(9517..11376)
                     /locus_tag="IVO69_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7399595.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11494..12653)
                     /locus_tag="IVO69_000797"
                     /pseudo
     CDS             complement(11494..12653)
                     /locus_tag="IVO69_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(12960..14384)
                     /gene="araA"
                     /locus_tag="IVO69_000798"
     CDS             complement(12960..14384)
                     /gene="araA"
                     /locus_tag="IVO69_000798"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7399596.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14400..15098)
                     /locus_tag="IVO69_000799"
     CDS             complement(14400..15098)
                     /locus_tag="IVO69_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7399597.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15055..16716)
                     /locus_tag="IVO69_000800"
     CDS             complement(15055..16716)
                     /locus_tag="IVO69_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7399598.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16934..18334
                     /locus_tag="IVO69_000801"
     CDS             16934..18334
                     /locus_tag="IVO69_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7399599.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18586..19665)
                     /locus_tag="IVO69_000802"
     CDS             complement(18586..19665)
                     /locus_tag="IVO69_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7399600.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19751..20710)
                     /locus_tag="IVO69_000803"
     CDS             complement(19751..20710)
                     /locus_tag="IVO69_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7399601.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20722..22245)
                     /locus_tag="IVO69_000804"
     CDS             complement(20722..22245)
                     /locus_tag="IVO69_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7399602.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22255..22818)
                     /locus_tag="IVO69_000805"
     CDS             complement(22255..22818)
                     /locus_tag="IVO69_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7399603.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22824..23687)
                     /locus_tag="IVO69_000806"
     CDS             complement(22824..23687)
                     /locus_tag="IVO69_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7399604.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23701..24597)
                     /locus_tag="IVO69_000807"
     CDS             complement(23701..24597)
                     /locus_tag="IVO69_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399605.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24772..26043)
                     /locus_tag="IVO69_000808"
     CDS             complement(24772..26043)
                     /locus_tag="IVO69_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7399606.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26033..27550)
                     /locus_tag="IVO69_000809"
     CDS             complement(26033..27550)
                     /locus_tag="IVO69_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7399607.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27748..28638
                     /locus_tag="IVO69_000810"
     CDS             27748..28638
                     /locus_tag="IVO69_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7399608.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28711..29535)
                     /gene="nadE"
                     /locus_tag="IVO69_000811"
     CDS             complement(28711..29535)
                     /gene="nadE"
                     /locus_tag="IVO69_000811"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7399609.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29547..31019)
                     /locus_tag="IVO69_000812"
     CDS             complement(29547..31019)
                     /locus_tag="IVO69_000812"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7399610.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31656..32018)
                     /locus_tag="IVO69_000813"
     CDS             complement(31656..32018)
                     /locus_tag="IVO69_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7399611.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32241..32993
                     /locus_tag="IVO69_000814"
     CDS             32241..32993
                     /locus_tag="IVO69_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7399612.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33133..34056)
                     /locus_tag="IVO69_000815"
     CDS             complement(33133..34056)
                     /locus_tag="IVO69_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7399613.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34071..34343)
                     /locus_tag="IVO69_000816"
     CDS             complement(34071..34343)
                     /locus_tag="IVO69_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399614.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10188 a         7186 c         5938 g        11202 t
ORIGIN      
        1 ttgacctgag tattagagat acaaaacgga aaaatagctt ctcatatttt gccgtttttt
       61 gagctaatac cgaaggacag ctgattgaac accgtttatt cggaaaaagg gactgtgaca
      121 cgacttttgt cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt
      181 cttcagaaaa ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat
      241 cgtggtttcc tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc
      301 gtattgtaga caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca
      361 tcgctttttg aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga
      421 tcaggacatc tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt
      481 gcgaaatatt gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa
      541 caaaatcgtc tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt
      601 cgttcccata tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta
      661 ccattgaaaa ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt
      721 aacgaatact acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct
      781 tccctgcacc agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa
      841 aatcagtcat tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt
      901 tgtatggtgt atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat
      961 ccaacacttc aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac
     1021 ttgcaagttg agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat
     1081 cccctaatgt catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat
     1141 agcctagatt tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt
     1201 tccaaccagc ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag
     1261 tatgattgaa tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca
     1321 ataaacctaa acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca
     1381 tcacgatgat caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga
     1441 ttgaaagcat catcacgatg accccaatca atgtgactgc actagttaca aattgagtca
     1501 aattttgctg cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat
     1561 ccccatttgt atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat
     1621 catgacgtaa ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa
     1681 agttgaatac agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt
     1741 tatcaaagtc tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag
     1801 ccattacacc tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt
     1861 ggaagatcac ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag
     1921 acatatagcc aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta
     1981 caccgagatt tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac
     2041 gtgtgtttgt tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg
     2101 ataagtttcg tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc
     2161 atctagcaca aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac
     2221 ggtgatactt tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa
     2281 atccaacgct gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg
     2341 tgcaattgcc agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg
     2401 gctatctaag ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc
     2461 tttccaaatc atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt
     2521 tccagagaac agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg
     2581 agtcatgtca ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa
     2641 tcgagggatc aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac
     2701 gatctcgccc gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg
     2761 atatcgataa ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt
     2821 tttaggattt tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc
     2881 agctgaagct tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat
     2941 caagatctgc atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc
     3001 tgctatgtag tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt
     3061 catcattggc atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc
     3121 tttattcgct aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac
     3181 tctgacccct gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat
     3241 acttttgaat aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg
     3301 gatgacaact aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc
     3361 gccaatcaac gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg
     3421 gatttgagtc acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa
     3481 cgcatctttt gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag
     3541 tttctgtgat tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa
     3601 agaaaagccg atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac
     3661 ccctttatca ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat
     3721 caccatgaaa actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag
     3781 catctagttc ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt
     3841 gaattcactg attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga
     3901 atgcgcaatt gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg
     3961 atcatttttt actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg
     4021 tttgtgttct ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt
     4081 tcttgaggca cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc
     4141 ttccaaaatc tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc
     4201 atcggattct ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa
     4261 gtaataaaaa taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat
     4321 atccgctaat ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc
     4381 gattctcgca gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg
     4441 tgtcggcata tggattctcc tttctttcaa gatgacacat tgtcatatga cgctatgtca
     4501 ttataacacc tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga
     4561 tacattaaaa aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa
     4621 aatagccaca tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact
     4681 tcaataaatt gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt
     4741 acacaaaaaa ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat
     4801 taaaaaatct agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag
     4861 aagcattagc cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga
     4921 tggaagctct tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg
     4981 acaaaaaatc gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca
     5041 accttatcaa tatgacttga tagccttttc tttttcgagt aaactgattt tgttagtcat
     5101 agttataaca atcatagcaa taaaaggaga aggtaagcat atggcatctg ttttagtagt
     5161 caaaggtcat cccctaactg cagaagaatc gcgcacagtt aaggcattga cgtctttttt
     5221 aacaagttat aaagaaaatc atccagatga tgaagtgact gttttggaac tttatcgcga
     5281 tgatattccg gaaatcgatg aagaattatt atcaggttgg gaagcattac gtgctggtgc
     5341 agaattcact tccttatcag aaagtcaaca acaaaaaatt gctcgtttca atgaattgac
     5401 tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac gccttatgga atctaaatat
     5461 cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta
     5521 tacagaaaac gggccagaag gattagtgac aggtaaaaaa gcactgcaca tccaatctaa
     5581 tggcggtgta tacaacggac aagactttgc ctctcaatat gtaaaaggta ttttaaactt
     5641 tgtaggcatc gatcaagtag atcagctatt catcgaagga atcgattatg accctgaccg
     5701 tgcagatgag ttgatgcaaa atgcattaga taaagctgct gctttaggta aatcatttta
     5761 aaaattgaga taaatgcata caaaagagac agaggaaaaa atcttcaaag cagcatttgt
     5821 gccttggata ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt
     5881 tcttcataaa gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta
     5941 gctgctgcat aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa
     6001 gtcagcagaa ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat
     6061 ggagagatca agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg
     6121 atcatgaatg gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat
     6181 cctaagtaag ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct
     6241 aaaccaatga ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt
     6301 tgtcctctta tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt
     6361 ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc
     6421 aatagataaa ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg
     6481 ccgacaaaac ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct
     6541 gccgaattca tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc
     6601 gggtcccgca aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta
     6661 ctgatgaagc tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg
     6721 attagtgcaa caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc
     6781 ttttcaaagt aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata
     6841 ggcagaccta cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta
     6901 aataaaaaga cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta
     6961 tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata
     7021 attcctcctg ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg
     7081 gatgaagata gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg
     7141 atacgtgagc tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat
     7201 atacctgatc atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg
     7261 atacttcttg aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa
     7321 tcagcatggg ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa
     7381 tcattgcact ctctccttta attgctttca aaaacagtat aacacattct cattctttag
     7441 ccgtgtggaa taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg
     7501 aatcagctca tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag
     7561 gaatctatac gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg
     7621 ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta
     7681 ctagtgtgga tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg
     7741 cggtaacaga gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac
     7801 tatacggggc gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg
     7861 aactgaaagc agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag
     7921 atcatgcgca tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc
     7981 ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa
     8041 ctaccttcac tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac
     8101 aattcgctta tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag
     8161 aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg
     8221 aaaatggagg ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc
     8281 gaggacataa ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca
     8341 agcaaatcat ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata
     8401 attttgtcgt gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg
     8461 aaacaggatt actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt
     8521 attttgatcg ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac
     8581 tacgggaaga actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg
     8641 aagaggaata tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga
     8701 atgtagaaaa ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc
     8761 cgtctatcct gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg
     8821 aaaaggagta tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc
     8881 attatcataa aggattttga tcactctatc ataaaggtaa tattacattt atattagata
     8941 actctagttt ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt
     9001 ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg
     9061 aatagatctt catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct
     9121 gctacatatg aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt
     9181 ggacgcaaag tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat
     9241 aacaggatga tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt
     9301 gcgtttctga tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg
     9361 acgatttgat cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta
     9421 ctttctccat tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt
     9481 gaaaaaattt aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc
     9541 tgctaaacta tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta
     9601 aatctcttgt tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta
     9661 cagttctcgt tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc
     9721 atttgttgtc tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat
     9781 tgcatcgtga gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt
     9841 ttcatgcaat gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag
     9901 atgatccccc caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa
     9961 ctgcttcagt gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt
    10021 cccacttcca tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg
    10081 catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag
    10141 tgtcattact tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc
    10201 cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc
    10261 ctctctttta tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc
    10321 gtttaacgct gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg
    10381 ggtggtgagt tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat
    10441 atttgctgtt ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc
    10501 tacttcataa taatctgcca atggctcacc agatacttct acgccgttta atcgactagg
    10561 atctgagaaa ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt
    10621 atttttttca tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga
    10681 atatccttca ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa
    10741 accagtgtta tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata
    10801 tgcttttcgc aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat
    10861 tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg
    10921 aaacatgtac cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa
    10981 cagcatagtg ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc
    11041 tggataaatc ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc
    11101 gatggctacg acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa
    11161 aataagaata atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa
    11221 agcaaatttc aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt
    11281 actaaaaaca acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt
    11341 ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca
    11401 ataaaagaat gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa
    11461 gaagaattaa tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa
    11521 gcggaccttt cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc
    11581 aaaatcagta ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga
    11641 tatatctgcg atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt
    11701 ggttttcttt tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga
    11761 tggataattg accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac
    11821 tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata
    11881 agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga
    11941 tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca
    12001 ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt
    12061 gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct
    12121 aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc
    12181 atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga
    12241 ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt
    12301 tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg
    12361 gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt
    12421 gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca
    12481 acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta
    12541 ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt
    12601 aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc
    12661 cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact
    12721 tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg
    12781 acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga
    12841 taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag
    12901 caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt
    12961 tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt
    13021 gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca
    13081 ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct
    13141 tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa
    13201 tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga
    13261 tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc
    13321 gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact agctagctcg
    13381 taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga
    13441 tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa
    13501 cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta
    13561 aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt
    13621 acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat
    13681 tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt
    13741 acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact
    13801 ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt
    13861 ccaaaacttt cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc
    13921 tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg
    13981 aacccatatt cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc
    14041 gttttccaag gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga
    14101 agctttgtcc cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca
    14161 cctgctactt cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta
    14221 actgctaatt cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg
    14281 accatttctt gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt
    14341 gatcctacaa caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt
    14401 tatttgtttt gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa
    14461 atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg
    14521 catacttcat ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta
    14581 aatggaccgt gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct
    14641 ttgaatgttt ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt
    14701 tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc
    14761 atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg
    14821 acgattcctc cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa
    14881 gagggattga gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct
    14941 gctgtcatct cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga
    15001 tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga
    15061 tttgcttggt atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg
    15121 caattgatga atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat
    15181 ctgatagcct gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa
    15241 cacgtcttct gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc
    15301 taataacgcg attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc
    15361 ctccatagca gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg
    15421 ccccactaaa cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat
    15481 tgcaccaaaa gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc
    15541 tggtgtacgt acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa
    15601 ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa
    15661 taccgtgaat aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa
    15721 aatactgacc caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag
    15781 ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc
    15841 taatacgatc atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac
    15901 actgttcgta gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc
    15961 tggtctcaaa tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc
    16021 ttcttctcct gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc
    16081 tttttcgatt aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg
    16141 atcgatagga aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg
    16201 ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt
    16261 attcaaaata gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa
    16321 tttttccgtt aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg
    16381 tactagtaac tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc
    16441 aatcgacccg attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt
    16501 tcggtaactg acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt
    16561 ttctagtttg ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat
    16621 caatacagct ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat
    16681 gacatctaac atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg
    16741 tcaaacctga tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta
    16801 cgtacatgtc aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca
    16861 taagttataa aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa
    16921 ggagagaata cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg
    16981 cgcatttggc ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt
    17041 tttacagcat gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc
    17101 ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg
    17161 acgacgaaaa atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc
    17221 atctgcccct cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc
    17281 tgttggcgct gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact
    17341 aaggggaaga ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta
    17401 tgttgcagat tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg
    17461 gctcgctgct gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc
    17521 gcgttttctt atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg
    17581 gacaccaaaa gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa
    17641 gacaaccgga acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc
    17701 cggtattggt gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat
    17761 tccgctgatc gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat
    17821 catccaagga atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt
    17881 caatcggcga actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc
    17941 agctgtttta ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt
    18001 atgtatttat gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg
    18061 cgaaatattc ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg
    18121 gattggttct ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga
    18181 aatcgttttc ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat
    18241 cgtaccagaa actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac
    18301 ttatcctaaa cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct
    18361 tcaagaaata agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc
    18421 ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg
    18481 gagaggtggt gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc
    18541 gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc
    18601 tttcactgtt tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc
    18661 tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg
    18721 cgtcaatgtt gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac
    18781 gatcgatagg tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact
    18841 agctatttga tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg
    18901 tactgttttt tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt
    18961 caaaaaacgc acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc
    19021 ttgaagatcg tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata
    19081 ttctgctgcc ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc
    19141 ttcataagca gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag
    19201 tgctaagttt gggttatact gattactttt cgtaggctca acgatcaaac catctacccc
    19261 ataagcaatc attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc
    19321 taaaagcaat gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg
    19381 aaaaatataa tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg
    19441 tgttttttct ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag
    19501 atagccctca tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata
    19561 tatttcttgc agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag
    19621 gatcttttgt tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt
    19681 ttatttgtac gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg
    19741 tttcttgtgc tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat
    19801 tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt
    19861 ggttcttctc tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg
    19921 taaacgagtt gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct
    19981 ccgataattt atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca
    20041 ggcccatggc atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat
    20101 tgcgagtaag gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact
    20161 caggtataga aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata
    20221 gataaagatt agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct
    20281 ttccttgaga agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt
    20341 gtcccttttc tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca
    20401 tgcggtgatg atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat
    20461 accattttcc attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt
    20521 cttcatgagc ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg
    20581 cccttcttaa ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat
    20641 ctgtatgttt atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg
    20701 tcttcatcat aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt
    20761 ttctatccct acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt
    20821 tattttttct ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta
    20881 gtgctctcct tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt
    20941 tgtgcgattg atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac
    21001 ataaggaaca ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa
    21061 tacttgacct ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca
    21121 tgcttgtcct ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca
    21181 actaatcttt acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa
    21241 tgtatcttcc atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg
    21301 tcttttttcc ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc
    21361 acttctcaaa tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc
    21421 gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt
    21481 agtcaagtct tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt
    21541 ctcataagct tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt
    21601 agagcttcca actaagattg tttcaattga atcatccact aacttcatga cttttgaggc
    21661 ttcatttgct aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc
    21721 tagctcattc cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat
    21781 tcttaaatca ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc
    21841 ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact
    21901 gatccattta aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa
    21961 atcaattttt tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga
    22021 tgtaaagttt cctccaggat agcgaatgac acctaattct aattccttta cataagagag
    22081 gacatcttgt ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt
    22141 atagacgaca cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc
    22201 taccgtaaaa tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct
    22261 ttcatttcct atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa
    22321 aataaaagga gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag
    22381 aatatttctc aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa
    22441 tgtgatatct ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat
    22501 aagtaaaact acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt
    22561 aacaagaaaa taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg
    22621 ctttattcct tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc
    22681 cattacataa aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat
    22741 tacccagaga atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt
    22801 agtctcaact attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca
    22861 aaatatttct gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca
    22921 gccattacag gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt
    22981 aaagttctca tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta
    23041 ttcatcagcg tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt
    23101 ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct
    23161 ggtaaagtaa taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga
    23221 atgatcaaac ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga
    23281 atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat
    23341 tttcctttga aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa
    23401 gtgatcactg tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc
    23461 gttgtccaaa ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga
    23521 aatgagataa tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac
    23581 gcaataacca acaatataac tagcatacta atcgttcgaa tgattttttt accactaact
    23641 gcttttcgac tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc
    23701 ctatttcatt tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc
    23761 aacaatgata aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt
    23821 aaatcctcga ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc
    23881 ggttgcaaca tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca
    23941 aaaacctaag gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt
    24001 tgcaccatcc atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa
    24061 gagaatcgtc atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat
    24121 agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta
    24181 aggaatcgcc cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa
    24241 tgaacaaata actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt
    24301 agataatgct actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt
    24361 gaaatataaa gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa
    24421 attttcaaaa cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat
    24481 agcaaatgtt cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt
    24541 taaaaacaaa tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata
    24601 gtccacctca tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa
    24661 gatatccgaa caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat
    24721 cattcagata tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg
    24781 cagtaattga ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc
    24841 taataatttt tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa
    24901 acctggtaaa tatggctctg catagtccat ttgtttcgat aattcagata caataggatc
    24961 attttttact tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat
    25021 actccatttt ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc
    25081 ttttttatta ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt
    25141 ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc
    25201 tttcaatccg ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat
    25261 taaattgtcc atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat
    25321 tttttggatc gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata
    25381 atcatcgttc actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc
    25441 atccttattg aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat
    25501 ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa
    25561 caaatcatta ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt
    25621 accttctact tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc
    25681 atattcccag aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc
    25741 tggataattt aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt
    25801 ttcattcaaa ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt
    25861 tttattaaat tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt
    25921 ccaaaaagtc aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca
    25981 agctcctaaa atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt
    26041 cattttttgt tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt
    26101 tcaataaaca attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta
    26161 cttgttcact tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat
    26221 caatcactcc ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc
    26281 cacggttaac tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat
    26341 acggcacttc ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca
    26401 cctctccttg tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag
    26461 cttctccgtt tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag
    26521 caatctttac tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg
    26581 catcttcaaa attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt
    26641 gctcatcttg ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct
    26701 gcttcttact atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga
    26761 accgatccat gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat
    26821 aatattgatg cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact
    26881 cccaatcacc aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg
    26941 aatcatctac gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg
    27001 ctgttttatg tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa
    27061 ttccaaaagg gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag
    27121 gaaaattaca atattcgact aaattacgag ctgcatctac tccacgagta cccagattga
    27181 cagccatatt gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa
    27241 cttcattcgt ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg
    27301 gcccaatact gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca
    27361 tgggaatgtt caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag
    27421 ctgatggatg atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa
    27481 atgaactgta caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg
    27541 tggcttccat aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt
    27601 tgtcaataat ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat
    27661 taataatgat taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata
    27721 agaaaaacct tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg
    27781 gtatatgaag cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa
    27841 aagatgaatg tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg
    27901 cacgtgaaaa agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa
    27961 atttcgagcc ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct
    28021 tttgatacga aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca
    28081 acatgcggtt tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt
    28141 atggatcctc gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat
    28201 caggcaccta attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa
    28261 atttcttctg aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta
    28321 aatggtgtgg agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa
    28381 tatacgcctg attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga
    28441 attaccaaac acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac
    28501 atccctaaag agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat
    28561 gtggggggct gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta
    28621 aaagtttact acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc
    28681 tatgaaggga cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat
    28741 tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg
    28801 agcgggaact tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc
    28861 gacttcatct gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta
    28921 taatttttcc ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg
    28981 gaataatggc aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt
    29041 ttctgcagca tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg
    29101 cgtaatcata cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc
    29161 tgcttcgctt aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact
    29221 aacatcgggc tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc
    29281 ataaggaaga cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc
    29341 ttccatcgtt agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc
    29401 tagtacaaaa gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact
    29461 tttacgtatt tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc
    29521 tgtctgcagg ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt
    29581 ttcattcgtt ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg
    29641 tatttttgcg gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt
    29701 gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag
    29761 atatcttgta aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc
    29821 actggatgga acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg
    29881 tagtctcctt cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct
    29941 ggagtagtta ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca
    30001 tcatcttcga tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg
    30061 atcaattttg tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa
    30121 atcgttgctt catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca
    30181 tctaattgtt cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg
    30241 ccttggaagt tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct
    30301 gatttcaacg tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag
    30361 gccataaatg cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg
    30421 ctagctggta tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg
    30481 atatatgccg ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca
    30541 aattccaata gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc
    30601 aacgtttgga aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa
    30661 ggcccttcca cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa
    30721 cgaactgttg ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact
    30781 tctaatccac gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg
    30841 actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag
    30901 taacattcaa aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata
    30961 ttgatttgat atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc
    31021 ttgtattact cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata
    31081 ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa
    31141 aaatgaactc catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag
    31201 aatacttatt ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt
    31261 acttgataaa ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg
    31321 aacaaatata accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa
    31381 tacgtctttc cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg
    31441 aatttttttg gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa
    31501 aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa
    31561 acaaaaatgg cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg
    31621 gagcaggtta ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt
    31681 tgttctatca ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc
    31741 tcctgtttca ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga
    31801 atcgcatagt cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc
    31861 tttttatcat atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa
    31921 taattaatag ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata
    31981 tttaaattac tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag
    32041 tcgatttaga tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta
    32101 caattgaacc tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt
    32161 tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac
    32221 atttgtcagg aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag
    32281 tcagtccgag aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca
    32341 attcttcagg ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat
    32401 tttatcgaac aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt
    32461 ttgaccacca gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa
    32521 tcgataccct cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa
    32581 tgagtgacaa agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat
    32641 cctatggaca agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa
    32701 aatggatgaa tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt
    32761 tatttgatct cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct
    32821 tccaagctca taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata
    32881 aaggactagg aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa
    32941 gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta
    33001 catgatccaa caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag
    33061 ttagattttt atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta
    33121 ctttaaatat attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc
    33181 tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg
    33241 gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc
    33301 ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc
    33361 aactccaagc tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc
    33421 ccaaaaaaca agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat
    33481 ttttgagtta taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc
    33541 ttgctgcttc aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca
    33601 gcttgcctgt tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc
    33661 caataaatca tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc
    33721 tgcaaattct tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt
    33781 cactttgtaa tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata
    33841 cggatgattg gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg
    33901 tctggcattt gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct
    33961 tttttcctca tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata
    34021 acctgtgagc tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa
    34081 agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt
    34141 ccttcatgcg aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg
    34201 atttcattca ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac
    34261 gttaatacgg atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct
    34321 gttatctcta cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat
    34381 gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat
    34441 tattaccaaa gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt
    34501 tattattagt gaat
//
LOCUS       DACCPY010000019        32689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCPY010000019 DACCPY010000000
VERSION     DACCPY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.019"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            191..421
                     /locus_tag="IVO69_000817"
     CDS             191..421
                     /locus_tag="IVO69_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7399615.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="IVO69_000818"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="IVO69_000818"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7399616.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="IVO69_000819"
     CDS             1832..2008
                     /locus_tag="IVO69_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7399617.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="IVO69_000820"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="IVO69_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7399618.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="IVO69_000821"
     CDS             2624..3091
                     /locus_tag="IVO69_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7399619.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="IVO69_000822"
     CDS             3176..4060
                     /locus_tag="IVO69_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7399620.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4177..4410
                     /locus_tag="IVO69_000823"
     CDS             4177..4410
                     /locus_tag="IVO69_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7399621.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="IVO69_000824"
     tRNA            4498..4578
                     /locus_tag="IVO69_000824"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="IVO69_000825"
     tRNA            4585..4656
                     /locus_tag="IVO69_000825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            5171..5878
                     /locus_tag="IVO69_000826"
     CDS             5171..5878
                     /locus_tag="IVO69_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7399622.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="IVO69_000827"
     CDS             complement(5937..6581)
                     /locus_tag="IVO69_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7399623.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="IVO69_000828"
     CDS             6707..6994
                     /locus_tag="IVO69_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399624.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="IVO69_000829"
     CDS             7102..7422
                     /locus_tag="IVO69_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7399625.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="IVO69_000830"
     CDS             7503..8816
                     /locus_tag="IVO69_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7399626.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="IVO69_000831"
     CDS             8813..9532
                     /locus_tag="IVO69_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7399627.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="IVO69_000832"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="IVO69_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7399628.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="IVO69_000833"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="IVO69_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7399629.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="IVO69_000834"
     CDS             join(13093..13155,13157..14194)
                     /gene="prfB"
                     /locus_tag="IVO69_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7399630.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="IVO69_000835"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="IVO69_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7399631.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14969..15862
                     /locus_tag="IVO69_000836"
     CDS             14969..15862
                     /locus_tag="IVO69_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399632.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="IVO69_000837"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="IVO69_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7399633.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17799..18656
                     /locus_tag="IVO69_000838"
     CDS             17799..18656
                     /locus_tag="IVO69_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7399634.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18771..19691
                     /gene="pstC"
                     /locus_tag="IVO69_000839"
     CDS             18771..19691
                     /gene="pstC"
                     /locus_tag="IVO69_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7399635.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19691..20575
                     /gene="pstA"
                     /locus_tag="IVO69_000840"
     CDS             19691..20575
                     /gene="pstA"
                     /locus_tag="IVO69_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7399636.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20590..21396
                     /gene="pstB"
                     /locus_tag="IVO69_000841"
     CDS             20590..21396
                     /gene="pstB"
                     /locus_tag="IVO69_000841"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7399637.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21412..22170
                     /locus_tag="IVO69_000842"
     CDS             21412..22170
                     /locus_tag="IVO69_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7399638.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22183..22860
                     /gene="phoU"
                     /locus_tag="IVO69_000843"
     CDS             22183..22860
                     /gene="phoU"
                     /locus_tag="IVO69_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7399639.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23097..24128
                     /locus_tag="IVO69_000844"
     CDS             23097..24128
                     /locus_tag="IVO69_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7399640.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24125..24892
                     /locus_tag="IVO69_000845"
     CDS             24125..24892
                     /locus_tag="IVO69_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7399641.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24992..26560
                     /gene="liaX"
                     /locus_tag="IVO69_000846"
     CDS             24992..26560
                     /gene="liaX"
                     /locus_tag="IVO69_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7399642.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26860..27177
                     /locus_tag="IVO69_000847"
     CDS             26860..27177
                     /locus_tag="IVO69_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7399643.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27177..27527
                     /locus_tag="IVO69_000848"
     CDS             27177..27527
                     /locus_tag="IVO69_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7399644.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27694..28632
                     /locus_tag="IVO69_000849"
     CDS             27694..28632
                     /locus_tag="IVO69_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7399645.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28647..29477
                     /locus_tag="IVO69_000850"
     CDS             28647..29477
                     /locus_tag="IVO69_000850"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7399646.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29521..30546
                     /locus_tag="IVO69_000851"
     CDS             29521..30546
                     /locus_tag="IVO69_000851"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7399647.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30561..31499
                     /gene="galU"
                     /locus_tag="IVO69_000852"
     CDS             30561..31499
                     /gene="galU"
                     /locus_tag="IVO69_000852"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7399648.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31624..32064
                     /locus_tag="IVO69_000853"
     CDS             31624..32064
                     /locus_tag="IVO69_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7399649.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32067..32669
                     /locus_tag="IVO69_000854"
     CDS             32067..32669
                     /locus_tag="IVO69_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7399650.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10850 a         5544 c         7087 g         9208 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaagct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg acgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagtt tcaggaggtc tctttgactt agatcagctg gaagaagaca tcgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgacagc gaagccgcac aaaaagtgat
    13261 cagtgaaaat aacgctaata aagaaaccta tgaccaattc cattctcttg ccgaagaact
    13321 agaagaaatc gaattgatgg tggagatgct gcaagaagaa cctgatgcag caatgcaaga
    13381 agaagcagaa acacgtatca atgcattaga tgaaaaaatg aagacgtatg aattatctat
    13441 gctgttagac ggaccttatg atcgaaacaa tgcgatcgtc gaattgcatc caggggctgg
    13501 cggaacggag tcccaagact ggggcagcat gcttttacgt atgtatacac gttgggcaga
    13561 gcagcatggc tatcaagtag aaacactaga ttatcaagca ggtgatgaag cagggatcaa
    13621 gagtgtgacc ctgctgatta aaggacataa cgcttttggt tatctaaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgga tttcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgctcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatccc tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcgattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgagatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatcgtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacgttga ctttagtcgg tgtattcatg gcagttatca tgaatgcaac
    15121 taaattagct caggatatag aaggaaacgt tgatgtatct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagtaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagagg aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atggctgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaagcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaaa
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatatc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctgatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactga tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcatt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacacaaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt caaagccagt atagcataga aacttttaat gagatagttt tttttacaat
    17701 tatgaggatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg
    17761 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
    17821 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
    17881 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
    17941 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
    18001 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
    18061 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
    18121 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
    18181 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
    18241 tattgaaccg tgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
    18301 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
    18361 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
    18421 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
    18481 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
    18541 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
    18601 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
    18661 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
    18721 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
    18781 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
    18841 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
    18901 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
    18961 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
    19021 ctatgatcac aggttcgttt attgtgacct ttttatctgc tattgttgcc acaccatttg
    19081 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
    19141 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
    19201 cagttatcgt gccttttgtc agaagtattt ttggcgggac agggtttggt atcttagctg
    19261 gtacatttgt gttgttcgtt atgatcttgc cgactgtcac aacgatgact gttgatgcat
    19321 taaaggcagt accacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
    19381 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
    19441 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
    19501 cattgatgcc aacaggactt actactcctg cttctacatt gacgtccatc ttaacaatgg
    19561 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
    19621 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
    19681 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatgtagt
    19741 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
    19801 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
    19861 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
    19921 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctgac
    19981 agatgtgatt cgcacgtcga ttgaaatctt gagctcattg ccttctgtcg ttgtcggttt
    20041 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
    20101 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
    20161 agcggtccat tacacacaac gagaagcagg attggcttta ggtttgtcac gctgggaaac
    20221 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
    20281 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
    20341 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
    20401 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
    20461 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatc attgctgttt tactgttcaa
    20521 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
    20581 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
    20641 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
    20701 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
    20761 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
    20821 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
    20881 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
    20941 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
    21001 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
    21061 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
    21121 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
    21181 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
    21241 atcgtgacgc ataacatgca acaagcggct cgtatcagcg actatactgc ctttttctat
    21301 ctaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
    21361 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
    21421 gaaattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
    21481 atcgatctaa gtttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcggg
    21541 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
    21601 accgggagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
    21661 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
    21721 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
    21781 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
    21841 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
    21901 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
    21961 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
    22021 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
    22081 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
    22141 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
    22201 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
    22261 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
    22321 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
    22381 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
    22441 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
    22501 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
    22561 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
    22621 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
    22681 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
    22741 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
    22801 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
    22861 actaaagcgg tgattctgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
    22921 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaatagctcc tcatattttt
    22981 catttggctg gacttattac cgcagatgca agcttttgaa cactgtttat tcgggaaagg
    23041 gtttgtgaca aaatatgtca caaacccttt tcatcatttc atagaaagga taaagaatgc
    23101 aaaaaaaaat tagcatcatc actatatttt ttatttgtgt gcttacttca tgggttattc
    23161 agcatcaagc tgctttatat gaagtacctg tcatcgagat cgaaaaagta cgaataaaag
    23221 aggatcatca attgattaca ggaagactga tgaacacgaa tcaaaaagga gaatcgattt
    23281 atttatcggt tccttatcaa aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa
    23341 tgtatctagt ccaaatgcat ggagatgaat ggcagctagt agcaaaaaaa aatgatggct
    23401 ggcttttctt cttcacaagt atcttcattg gcgccatgtt gcttattggc ggaaaacatg
    23461 gtctatttgc tttaggaagt ctcatcatca atatcgtttt acttttactt ttgttgctca
    23521 tctttactaa aatagaaggg atttctctat taaatatatc tatcctgttc gtcgttatag
    23581 ggatgattat tagcatattg gctttagatg gatggaatag aagcagtata atcaaaattt
    23641 cagctgcagt tacgagtgtt tttttagcat tttttatttg tcttttgaca atgaatcttt
    23701 ggaaagacca aggactgcgc tatgaggaac tgaattatct gacacgaccg tatcgttctg
    23761 tttttctttc tagcgtactg attggaacag ctggaggaac actcgatacc gttatcacag
    23821 tcattgcgac attagaagaa ttgactaagc gacaaccaga aataacagcg aaacagttat
    23881 ggctttcggg aaggcaagtg ggtcgagatg tgattggctc gatgaccaat atccttttgt
    23941 tcgtctatat cagtgggtca gttccatcgc ttcttttata tttaggaaat caatggtcgt
    24001 tcaaagaaac gatagaacag catttatcat tggaattttt acgcgtcatt gctggcagct
    24061 taggaatagt attatcgatc cctatctcgg tcctcttttt tctgactgta aggaggctga
    24121 aacgatgaat gtacttatta tacttgttgg gattttagca atcagcgtgc tacttattgg
    24181 cggtacgcaa ggtatgtata ttcttttagg cttattcatc aatttaggga tctttttctt
    24241 gttgttattc ggttatcatc agaagtggcc gatactggca ttgtcggtca taggattttt
    24301 attgattgct gtggttatct tattttttat caacggttat aatctaaaaa tgcgtgcagc
    24361 ttttgtgagt atactgatct ttttattttg tttcctacta ttgcttccta tcactgattt
    24421 tttagcaatc caaggtttta caacgataga actagaagaa ttatctggct tagataagac
    24481 tttagcgatt gattttagat tacttacgag atcgttgctt ctgattagtc ttagtggtgc
    24541 tgttttagat gcgagtgtag caatttctag tgggacattc gaagtatatc aggcaaatcc
    24601 gcaattatct ttttaccaat tgcgccattc tagtttcgct attgctaaaa aaatattggc
    24661 gtcaacagta atgacacttc tttttgcatt catgggaagt tctttagcgt tgatcctctg
    24721 gtttattgat ttagacattc cttttcagca agtgatcaat gaaaaatcat ttgttctaga
    24781 atatacgatg gctattctta ccacattgtc cgcactactt gttcttcctt taacctgcgt
    24841 gactacagca tatctcttta caaaaaagaa agaacaactc aagatggagt aaaagttaca
    24901 acttaagtcc tatgacattt ttactgaatc agtgcataat atggatgtaa ggaaaagctt
    24961 gcaaacaatt ttgcttaagg aggcaatttc catgaaagaa agagaacgta ttttagattt
    25021 agtgaaaaaa ggaatccttt cgactgaaga aggcttagat ttattagaaa gcatggcaac
    25081 tgaaaaagac gaaaagcaaa tcaaaaaaga agcagacaag gtaactgcta gtcataaaga
    25141 aaaagatcag gcaagtcaat tgatcgataa acttgaaaac ggcgaagaag aaatttctga
    25201 gccagttgat ccaaaggaaa gagaaagaca agatcaagaa aacttagaaa agatcttaga
    25261 tgaactagca actgaagcga ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt
    25321 cgctgacaat aaggcagcac gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa
    25381 agaagaatta ggcgagttga cagaagaaga attagcacag cgtcagacgt tagaagcaga
    25441 aatcaaagag ttagaagcta gtggcgatac actgttagaa gaacaattga aattagaagc
    25501 agaattaaaa gatatccgta agaatcaatg gtctgagaaa aaagaaacgt ttactgataa
    25561 gtttgaattg ccagatgatt ggaaagatca agcaacagat acactgaatc aagtaggcga
    25621 aaaaatgtca gaagctggat cacagttagg aaaattcttg aagaaaacat tccaaactgt
    25681 ttcagaaaca gtgaatgaca acatggaatg gaaagatgtc agcttgcgtg tccctgggat
    25741 tgcgacaacg aaatttgagc atgaatttta ctatgaagct cctgcagcaa gtatccttga
    25801 tatcaaggca gcaaacggaa acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt
    25861 tgaagctaag atcaaacttt acggaaaaat gggtgctgaa ccatttgaag cattctctga
    25921 acggagccag attgaggtta atgaagatca tatctccttc caaattccaa ataaacgtgt
    25981 gcgtgcagac cttgttttct acttacctaa acgagtatac gaccacgcag ctattaaact
    26041 tctaaatggc aacatcatga ttgaaacatt ggaagctaaa gatatttata caaaatctac
    26101 gaatggtaac atcatcgtgg accaattaac tgcaacgatg ctggaagtag aaggtgtaaa
    26161 cggcaatatc gatattcgaa acgggaacat cttagattct attatcgaaa cagtaaatgg
    26221 tacagtgaca tttggtgcga cagcagaaaa cctttctgtg tctttggtaa atggtgatgt
    26281 acgtcttacg attaaagaag ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa
    26341 tgtcaaagtc gctttaccag atggcatcgg tttggaagga catgctaaaa caagtcttgg
    26401 aagtatcaac agccgtctgt ccaattacga agtagttcgt gagaaaaaag aacggacaaa
    26461 ccaaatgttg caattccgtc gtgtttcaga tggtgaaatc gcgcaagtgc aactttcaac
    26521 gacaacagga agtatctatt taaaagatac agataaataa agaggttgtg aaagagatgt
    26581 tcagccttga gcaataagaa gcgattttgt aaaatagctt cccctatttt acaaaattgt
    26641 gccttattgc cgataggcta tctcttgaac accgtttacc aagagattat gaacttgcca
    26701 attatcgccg agtcacaagg aataatttca aaaaaagctc gtcacgtttt atgaatatgg
    26761 taatttgtga ccgagaattt tgcaaaaaga caccgattaa gaagaaggtt gtcacaacga
    26821 ttttcttcat ttaaaatagt agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa
    26881 tccaataaaa atgttgtttt gacagggact ttagcaggaa taggagaata catcgggatt
    26941 gatccgactg ttatccgtgt gctttatgta ttcctgagct tagttgcttt tggaagtcca
    27001 atcattttat acattttatt agctttgatt atcccatctg cgcgttctgc acgaacaaac
    27061 tatggacata acaatgcata ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa
    27121 acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg aatggagtga cttttaatgt
    27181 cgtactttca acgtatttta gttaatacgt taacattcat atcattggct gttctttttc
    27241 ctgcgatggt ccatgtgagc agtatttgga cggccgttct tgccagcttc gtattgtctg
    27301 tgttgaatat gtttgtcaaa ccgattttga cgatactgtc cctgcccttt acattagtca
    27361 cattgggatt gtttacgttt gttatcaatg cagcgattct taaaatgacc tccttttttg
    27421 tgggagcaga acattttagt ttttcttcat tcggtgcagc gttgttagta gctatcttga
    27481 tgtcgcttgt caatgtaatt gtcagcgaac ataacagtag aaaataaaag aaagctgtgc
    27541 cgaactatcg gcgcagtttt tttatttaga atcaggaatg cgttttctat tacattggta
    27601 attaaataaa aacgtttttt ttaattggta gtacttttgg ctagacaatg ataaaatgat
    27661 agagaacact ctaatagaaa tgaggtagca tgaatgtcag aagttgtaaa agttagccaa
    27721 ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc
    27781 atcgtaactg gggacatctc acgcccagga ctagagttga ctggttattt taattattat
    27841 tcccataatc gtttacagct atttggcagt aaagaaatca gttttgcgga acggatgatc
    27901 ccagaagagc gtttgatcat catgagaaga ttatgtagtg aagatatgcc cgcatttatt
    27961 atttcaagag gattaccagc tccagaagaa atgatccagg cagcaaatga aaatgggata
    28021 gctgtactgc gttctcccat ctcaacttct cgcttattag gagaattatc cagttattta
    28081 gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt tagtggatgt ttacggatta
    28141 ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc
    28201 aaacgcggtc atcgattgat cgcagacgat cgagtagatg tttatcagca agatgaatta
    28261 acggtgatcg gagaaccgcc taaaatcctg gaacatctga ttgaaatccg aggaatcggg
    28321 atcattgatg taatgaatct ttttggtgcg agtgcggtac gtggctctat gcaagtccag
    28381 ttggctgttt atctggaagc atgggcaaaa gataagaaat acgaccgtct gggctctgaa
    28441 gacacgaccg ttgaaatcgc ggaagtaggc attccacaaa taaaaatccc tgtcaaaaca
    28501 ggacgaaacg ttgcaatcat cattgaagta gccgcgatga atttccgtgc acgtacaatg
    28561 ggctttgacg caacgaaaac atttgaagaa agattaagtc gcttgattga agaaaactca
    28621 ggcaacgact agcaaggagt gacaatatgt tagcacaaat caatcgagtt gcttttgagc
    28681 ttgtagggat tcctatctac tggtatgcat tgatcatcgt atcaggtatc attatcgcca
    28741 tgtggcttag cagcagagag gctgttcggg taggattaaa agcagatgat gtgacagatt
    28801 ttatgttgtg gggattgcct atctccatta ttggcgctcg cttatattat gttttatttg
    28861 atcttcccca gtatatagct gatccaatcc aaatattcaa tatccgttca ggaggcttag
    28921 ctatttatgg cggactgatt gcaggcgcga tcacgctgta tttttttact aaatatcatt
    28981 ttatttcttt gtggactttt ttagatatcg ctgcaccaag tgtattatta gcacaagcaa
    29041 tcggtcgttg gggaaatttc atgaaccacg aagcatatgg accagacacg acgcgaagct
    29101 tcttagaaag tctgcattta ccgcaattca tcattgataa tatgtatatc gaagggatct
    29161 atcgtcaacc aacatttctt tatgaatcac tttggagttt gattggattt attttgcttt
    29221 tgtttttacg aaagaaacca caccttttga aaaaaggtga agtcctttta tgttatctaa
    29281 tgtggtatag tttcgggcgt ttctttattg aaggattacg tacagacagt ctttactttt
    29341 taggtgtgat ccgtatttct caattattat ctgctgtgtt atttgtcggt acattgattc
    29401 tatttatttg gcgcagaaaa aaaggaaatt taccggatta tgaccgctca ttaggaaaaa
    29461 atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa
    29521 atgaattata gagtagcggt tttaggacca ggctcttggg gtactgcttt ggcacaagtc
    29581 ctcgccgaaa acggacatga cgttcggatt tggggacatc gtgcttcaca agtagaagaa
    29641 atcaacaccc aacacacgaa tcaacgttat ttaccagatc attgtctacc cgattccatc
    29701 caagcattca ccaatatgga agatgcagta aaagacgcag atgcggtgct attcgtcatt
    29761 ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca
    29821 gtgatcatcc atgcaagtaa aggattagaa caggggacac ataaaaggat ctctgacatc
    29881 ttgttagagg aaatcccagt agaaaaaaga aaagctgttg ttgttctttc tggaccaagc
    29941 catgcagaag aagtagcgaa acatgacatc actacgatca cagctgcaag tacagatgca
    30001 aaagcagcaa aatatgtaca agagctgttc atgaataatt attttcggat ctataccaat
    30061 cctgatgtga tcggtgtaga gatgggggca gctttgaaaa atattattgc tattggtgct
    30121 ggcgctattc acggcttagg gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga
    30181 ttagctgaga tcagccgttt aggtgtagca atgggtgcca acccgctgac ctttatcgga
    30241 ttaagcggag taggcgatct agtcgttact tgcacaagtg ttcattctag gaactggcgc
    30301 gcaggcaact tgttaggaca agggcagccc ctagaagagg tgctagccaa tatgggaatg
    30361 gtcgttgaag gtgtgaatac tacccaagca gcgatggaac tagcccaaat aatggaaatc
    30421 gaaatgccca tcactcaaac aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca
    30481 gccgcaaaag aaatcatgtt acgtgatggg aaaaaagaaa atgaattctc attaacaatt
    30541 ttttgaagga gcgaatgtat atgaaagtca aaaaagcagt tattccagca gcaggattag
    30601 gaacaagatt tttaccagca acaaaagcta tggcaaagga aatgttgcca attgttgata
    30661 aaccaacaat ccagttcatc gttgaagaag cgttgaaatc aggtatcgaa gacatcttga
    30721 tcgttacagg aaaagctaaa cgaccaatcg aagatcactt tgattcaaat attgaattag
    30781 aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa attagtagaa gaaacgacag
    30841 atgtcaactt gcatttcatc cgtcaatctc atccaaaggg tttaggacat gccgttcttc
    30901 aagcaagagc ctttattgga aatgaaccat ttgtcgttat gctaggcgat gatataatgg
    30961 aagacgaagt accattgaca cagcaattaa tcgatgatta cgaacgtact catgcttcta
    31021 cgatcgccgt tatgcaagtt cctcatgatg aaacttcaaa atacggaatt atcaatccag
    31081 gagaagtatt ggaagatggg ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa
    31141 gcaaagcacc aagtgattta gctattattg gacgttactt attgactcca gaaatttttg
    31201 atgtattgtc cgagcaacaa ccaggagccg gaaatgaaat ccaactaact gacgcaatcg
    31261 atacattgaa taaaacacaa cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg
    31321 tcggtgacaa atttggtttt atgaaaacaa gtattgaata tggtctaaca catccagaag
    31381 taggtgcacc tttacgtgag tacatcctgt ctttaggtaa aacattagaa gcagaaggaa
    31441 gcaagatcga tcataatcca aaaggaataa cagaaatcaa agaagaaaag aaaaaataac
    31501 ccttttttct aacgagcttc agctgctaat tgaataaaaa gtagttgaag ctcgttttta
    31561 tttgtttaaa aaacacgaaa atgctatcct tatagaaaag agagacaaga agggagtttg
    31621 atcatgtcag gaggagaaat tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc
    31681 gtttttatcg ttcgtgtgtt gttgcaagtt tctaaaactt tagaaaaagt agatcagact
    31741 gtagcagaag caaatacgac gatcgaaatc gtcacaaaag acgtagatct tttgtctaga
    31801 caagtagaag gcttactggt caaaagtaat gaactgctct tggatatcaa tggaaaagtc
    31861 gcaacgatcg atccattgtt cacagcagtt gctgatttaa gtgaaagtgt atcagaactt
    31921 aatcattcta gtaaaaacat cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa
    31981 gcgacggttg ccggaaaagt cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat
    32041 actgaaacga caggaggaaa ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag
    32101 tagtcggagg cacagccgca gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag
    32161 aacttcggga tgaattagct gcccaagcgg atgatttgat ggatatggct tcagattaca
    32221 cagatatggc gaaagaaaaa tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag
    32281 atctttctca acaagcaggt gatctagcag gtagtgtcaa acaaaaagta agtgaatcag
    32341 cagatgaagc aggaagtgaa tctgacgata tcttgttaaa tcttaaagaa caatcagctg
    32401 acttatctaa tcgtttcaaa aaaacggcag aagatatgaa aaaacctgca gatgaattag
    32461 gaagtattgc taaagatact tcagaagata tcataattga tgtaaaagac gctgctggta
    32521 aagcaggaga tactgtcaat gaaggaatgt cagaagcaaa accagttgca gatgactcaa
    32581 aagaaatgat gaaaatggca aaagaagatg cgaaagaagc taaaaaagaa ataaaagata
    32641 caatgacaga ttcatctaca aatcactaaa atcacaaatt tatagaata
//
LOCUS       DACCPY010000020        31740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCPY010000020 DACCPY010000000
VERSION     DACCPY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.020"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IVO69_000855"
     CDS             363..731
                     /locus_tag="IVO69_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7399651.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IVO69_000856"
     CDS             911..1852
                     /locus_tag="IVO69_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7399652.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IVO69_000857"
     CDS             1921..2652
                     /locus_tag="IVO69_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7399653.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IVO69_000858"
     CDS             2796..4976
                     /locus_tag="IVO69_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7399654.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTELIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IVO69_000859"
     CDS             4996..5478
                     /locus_tag="IVO69_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7399655.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IVO69_000860"
     CDS             5494..6255
                     /locus_tag="IVO69_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7399656.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IVO69_000861"
     CDS             6386..7720
                     /locus_tag="IVO69_000861"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7399657.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IVO69_000862"
     CDS             8027..8512
                     /locus_tag="IVO69_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7399658.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IVO69_000863"
     CDS             8505..9188
                     /locus_tag="IVO69_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7399659.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IVO69_000864"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IVO69_000864"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7399660.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IVO69_000865"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IVO69_000865"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7399661.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IVO69_000866"
     CDS             12804..13433
                     /locus_tag="IVO69_000866"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7399662.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IVO69_000867"
     CDS             13485..13817
                     /locus_tag="IVO69_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7399663.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IVO69_000868"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IVO69_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7399664.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IVO69_000869"
     CDS             14643..16016
                     /locus_tag="IVO69_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7399665.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IVO69_000870"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IVO69_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7399666.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IVO69_000871"
     CDS             17071..17511
                     /locus_tag="IVO69_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7399667.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IVO69_000872"
     CDS             17655..18356
                     /locus_tag="IVO69_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7399668.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IVO69_000873"
     CDS             18353..19474
                     /locus_tag="IVO69_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7399669.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IVO69_000874"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IVO69_000874"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7399670.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IVO69_000875"
     CDS             20853..21761
                     /locus_tag="IVO69_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7399671.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IVO69_000876"
     CDS             21796..22068
                     /locus_tag="IVO69_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7399672.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IVO69_000877"
     CDS             22079..23125
                     /locus_tag="IVO69_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7399673.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IVO69_000878"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IVO69_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7399674.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IVO69_000879"
     CDS             complement(26136..26807)
                     /locus_tag="IVO69_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399675.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IVO69_000880"
     CDS             complement(26800..27294)
                     /locus_tag="IVO69_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7399676.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IVO69_000881"
     CDS             complement(27314..28534)
                     /locus_tag="IVO69_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7399677.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IVO69_000882"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IVO69_000882"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7399678.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31737)
                     /locus_tag="IVO69_000883"
     CDS             complement(30610..31737)
                     /locus_tag="IVO69_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7399679.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10566 a         5668 c         6792 g         8714 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaattgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctctaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
//
LOCUS       DACCPY010000021        31596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCPY010000021 DACCPY010000000
VERSION     DACCPY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.021"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IVO69_000884"
     CDS             complement(76..543)
                     /locus_tag="IVO69_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7399680.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IVO69_000885"
     CDS             complement(663..2201)
                     /locus_tag="IVO69_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7399681.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IVO69_000886"
     CDS             2281..2967
                     /locus_tag="IVO69_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7399682.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVO69_000887"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVO69_000887"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7399683.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVO69_000888"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVO69_000888"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7399684.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IVO69_000889"
     CDS             complement(4453..5583)
                     /locus_tag="IVO69_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7399685.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IVO69_000890"
     CDS             complement(5583..7766)
                     /locus_tag="IVO69_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7399686.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IVO69_000891"
     CDS             complement(7763..8788)
                     /locus_tag="IVO69_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7399687.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IVO69_000892"
     CDS             complement(8833..9660)
                     /locus_tag="IVO69_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7399688.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IVO69_000893"
     CDS             complement(9657..10724)
                     /locus_tag="IVO69_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7399689.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IVO69_000894"
     CDS             complement(10750..11586)
                     /locus_tag="IVO69_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7399690.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IVO69_000895"
     CDS             complement(11583..12452)
                     /locus_tag="IVO69_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399691.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IVO69_000896"
     CDS             complement(12522..13805)
                     /locus_tag="IVO69_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7399692.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IVO69_000897"
     CDS             complement(13819..15504)
                     /locus_tag="IVO69_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7399693.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IVO69_000898"
     CDS             15779..16804
                     /locus_tag="IVO69_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7399694.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVO69_000899"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVO69_000899"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7399695.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVO69_000900"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVO69_000900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7399696.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IVO69_000901"
     CDS             25476..26444
                     /locus_tag="IVO69_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7399697.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IVO69_000902"
     CDS             26462..27367
                     /locus_tag="IVO69_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399698.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IVO69_000903"
     CDS             27364..28170
                     /locus_tag="IVO69_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399699.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IVO69_000904"
     CDS             28327..29295
                     /locus_tag="IVO69_000904"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7399700.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IVO69_000905"
     CDS             complement(29612..30136)
                     /locus_tag="IVO69_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7399701.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31596
                     /gene="scm"
                     /locus_tag="IVO69_000906"
     CDS             30423..>31596
                     /gene="scm"
                     /locus_tag="IVO69_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7399702.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTD"
BASE COUNT         8873 a         6830 c         5948 g         9945 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    31561 cagatactac ctctaccaca agtgatagca cggaca
//
LOCUS       DACCPY010000022        31360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCPY010000022 DACCPY010000000
VERSION     DACCPY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.022"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..243
                     /locus_tag="IVO69_000907"
     CDS             4..243
                     /locus_tag="IVO69_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399703.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..549
                     /locus_tag="IVO69_000908"
     CDS             310..549
                     /locus_tag="IVO69_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399704.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            635..1306
                     /locus_tag="IVO69_000909"
     CDS             635..1306
                     /locus_tag="IVO69_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7399705.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1359..3335
                     /locus_tag="IVO69_000910"
     CDS             1359..3335
                     /locus_tag="IVO69_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7399706.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3348..4103
                     /locus_tag="IVO69_000911"
     CDS             3348..4103
                     /locus_tag="IVO69_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7399707.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4100..4924
                     /locus_tag="IVO69_000912"
     CDS             4100..4924
                     /locus_tag="IVO69_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399708.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5213..7324
                     /locus_tag="IVO69_000913"
     CDS             5213..7324
                     /locus_tag="IVO69_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7399709.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7362..9419)
                     /locus_tag="IVO69_000914"
     CDS             complement(7362..9419)
                     /locus_tag="IVO69_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7399710.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9533..9745)
                     /locus_tag="IVO69_000915"
     CDS             complement(9533..9745)
                     /locus_tag="IVO69_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399711.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9932..10399
                     /locus_tag="IVO69_000916"
     CDS             9932..10399
                     /locus_tag="IVO69_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399712.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10396..10638
                     /locus_tag="IVO69_000917"
     CDS             10396..10638
                     /locus_tag="IVO69_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399713.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10656..10958
                     /locus_tag="IVO69_000918"
     CDS             10656..10958
                     /locus_tag="IVO69_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399714.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11030..13084
                     /locus_tag="IVO69_000919"
     CDS             11030..13084
                     /locus_tag="IVO69_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399715.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13084..15696
                     /locus_tag="IVO69_000920"
     CDS             13084..15696
                     /locus_tag="IVO69_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7399716.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15693..18224
                     /locus_tag="IVO69_000921"
     CDS             15693..18224
                     /locus_tag="IVO69_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399717.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18276..18608
                     /locus_tag="IVO69_000922"
     CDS             18276..18608
                     /locus_tag="IVO69_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399718.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18609..19202
                     /locus_tag="IVO69_000923"
     CDS             18609..19202
                     /locus_tag="IVO69_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399719.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19365..21059
                     /locus_tag="IVO69_000924"
     CDS             19365..21059
                     /locus_tag="IVO69_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7399720.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21747..23624
                     /gene="ltrA"
                     /locus_tag="IVO69_000925"
     CDS             21747..23624
                     /gene="ltrA"
                     /locus_tag="IVO69_000925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7399721.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23755..24132
                     /locus_tag="IVO69_000926"
                     /pseudo
     CDS             23755..24132
                     /locus_tag="IVO69_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24132..25337
                     /locus_tag="IVO69_000927"
     CDS             24132..25337
                     /locus_tag="IVO69_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7399722.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25353..25997
                     /locus_tag="IVO69_000928"
     CDS             25353..25997
                     /locus_tag="IVO69_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399723.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26008..26814
                     /locus_tag="IVO69_000929"
     CDS             26008..26814
                     /locus_tag="IVO69_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399724.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26837..27066
                     /locus_tag="IVO69_000930"
                     /pseudo
     CDS             26837..27066
                     /locus_tag="IVO69_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27225..28160
                     /locus_tag="IVO69_000931"
     CDS             27225..28160
                     /locus_tag="IVO69_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP7399725.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCIMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            28204..28473
                     /locus_tag="IVO69_000932"
     CDS             28204..28473
                     /locus_tag="IVO69_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399726.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            28705..29001
                     /locus_tag="IVO69_000933"
     CDS             28705..29001
                     /locus_tag="IVO69_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399727.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            29003..29425
                     /locus_tag="IVO69_000934"
     CDS             29003..29425
                     /locus_tag="IVO69_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399728.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            29444..31036
                     /locus_tag="IVO69_000935"
     CDS             29444..31036
                     /locus_tag="IVO69_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399729.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31057..>31360
                     /locus_tag="IVO69_000936"
     CDS             31057..>31360
                     /locus_tag="IVO69_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399730.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT        11444 a         4846 c         6403 g         8667 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatgtgaaa aaaagttcta attgtcaact attgttagaa
      541 gtctcatgac attcattaat agaattcatt tttaaaactt tttctatgga aatgagaaaa
      601 agattttttg ttttgtctga aaggaggtag ctggatgaga aaatggttat ttggtttttt
      661 aggagtcgct ttaatcgtct tttgcagtgt gtttggctat gttagctacc aaaaacatga
      721 aggagaggtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca
      781 tgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat
      841 gttgtccata caaaaagaag caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat
      901 tcagattcct gcacttggtt tagcattgcc aatttttaaa ggtgcaaatc aatacacttt
      961 gagtcttgga gcggctactt atttttatga agatgctgaa atgggcaaag gaaactatgt
     1021 cctagcaggt cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc
     1081 attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg
     1141 taaatttatc gtacctgggt actttaaatt aatcgatggc gttcctgaag agaactcttt
     1201 cttgtctttg ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac
     1261 gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag gttttaatca
     1321 acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa
     1381 aaaaagctgg aatgcttgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc
     1441 aagtacatgc agaagatgtc acgcaaaaaa caccaccaga gaaggtcaat atcacggttc
     1501 ataaattaat gtacgatcaa ggaacgcaat tgaatgttga tattgacggg atcaaaaacg
     1561 atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg
     1621 gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgaga gaagattctg
     1681 atctaaccaa cgcgaaagtg gacgagattg tacaagatgt agaagacaag ggatctaaca
     1741 gtgagtacgt caagaatgca accaacaaaa ttacttcagc tgtagatgaa aatggtgaga
     1801 tcacttttac tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg
     1861 aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc
     1921 caatgacaga taaaacagga tcaggatttt taaaaaatat tcatttgtat cctaagaaca
     1981 ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcgca gaccaaagca
     2041 aacaaaccct tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa
     2101 caaaattggg tgatcttgta tcggatgatc aaggaaagct gactgcgact gatttgacgt
     2161 taggaaaata ctattttgtg gaagttcctt cggaagttgt agtgggatca gatcaagaac
     2221 caacagcaga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa
     2281 cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac gtaaactata
     2341 aagcaccagt gatcgataaa acagtaacaa atggaacagg acaagaacat tctttccaaa
     2401 ttggtgacgc agtaaattat caaggaacga ttcatattcc aacagacatt gcaggtggcg
     2461 cagacggtat cacagtaaac ggtgtgaaaa gcgaaacttc tccttacagt gtatttaaat
     2521 ggggagatac agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa
     2581 caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg
     2641 atagtggttt tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga
     2701 atttacatgg tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa
     2761 tcgcaaatcc attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg
     2821 aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag
     2881 tcgatagcgg cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg
     2941 cagctaagaa tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg
     3001 tcaaagaagc agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag
     3061 agggtcattt tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag
     3121 ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat
     3181 acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg
     3241 gtagcaacgg tttccaatct tacgtattga tctcttgctt gttgctagga gcagggggac
     3301 tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg attattcgta
     3361 agcccccaaa aagaagagtc atgcacaaac tgatgatatt tttttggttg ctgtcggcag
     3421 tagcagtcta tttaattcca gttgtggaaa caaatgtagc gaaatttcaa caacagcaaa
     3481 gaattttagc cattgaaaat cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag
     3541 agttaggcga tcctgttggt gttcttacaa ttccaagtat ttctttaaaa ctaccaattt
     3601 atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa ggaactggcg
     3661 atattacagg aggaaacggt aaaaatcctt taatcgcggg gcacagtgga ctttataaag
     3721 ataatctttt cgatgatttg ccttcagtaa aaaaagggca aaaattcttt attaaggtaa
     3781 atgaagaaca acatgcctat caaatttatc gaattgaaga agttaaagca gaagaattac
     3841 aaaaaaatta tttagattat cttatgcctg aaaaagacca agataggatc acgttaatga
     3901 catgcacacc aaaaggtgtc aactcacacc gctttttagt gtatggtgaa cgagtttcct
     3961 tcagtaaaga agatttaaaa ctacagaaaa aagcaaaaaa taggaaatca atttcattgg
     4021 attggcgatt agcactgcta ttcgcttttt tttgcgttcc tattattttt atttataaaa
     4081 aaataaggag gagatcggaa tgaataagag aaaatggctg tttgtttgtg tagcagctct
     4141 cttttctact gtagcttatt cgcaactgtc tttcgctgac agcagtgata gtagtaccac
     4201 tcaaacaata gataattcaa ttactgcacc aagcgaaacg gacagttcgg agaatactac
     4261 agaagaaacg gcagattcaa atataagctt tactgaatca acgaatccaa cgtcagaaag
     4321 ttcttcagag acttcagaaa agccgcaaga cgtagaagat acgcaagtga tcaagcaagc
     4381 cattcatatt caaaaaatta tttcttcgaa tcaaatcaac aatcaaggca gtcaagtagc
     4441 ccagcaagaa ggtatcaatg gcgcgaagtt tattgtctac gatatcacga atatcttaga
     4501 agaaatgtca caagatgata atgagaaaaa tcaatctgta gaaataattg agtcaaaatt
     4561 gaaagatcga gcaaaaaaac tttcatacga ccagttgaaa aaagtggctg aaggagaaac
     4621 caaaacgatt gatgatcagg aaggcgtgat tgaattttcc attgaagtac ctgcaaacaa
     4681 aaaacaagca tactatatcg ttaatgaatc aagccctgaa aacatttcaa acagtgaaga
     4741 tattgtttta ttgacacctg tttcaaatga aaaaggtgaa tttttgacgg atgtttggtt
     4801 ataccctaaa tcagagaaat cagagccgaa agaagaaatt aaaaaagttg tgtcaactgg
     4861 tgtaaagaag aacttttttg aaaattgttg ggaatttttt gctcaattat tttttgataa
     4921 ttaggatgaa atgatgaaaa taataagaat attagttttt aatctatcct ttttagccgc
     4981 aacaatattg tgttgcggtt taattttttc tcaattatca atttatccaa gatatcaacg
     5041 attgttcatt cattttctaa acattcaatg tgcttccatg ccattagagg tgattaaaaa
     5101 gctcttgatc aatccaattt ttatggttag tttggttctt cttacaggat tggtaacgtg
     5161 ggtattagga aaaaaagaat gaaaagctta cgatcgttta aaaaaatatt agatggaggg
     5221 cattatggaa aataaaattc attgcactac gggaagagta attactttag tgttgttttt
     5281 gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag ttgatccgag
     5341 tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat tgagaaatat
     5401 cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa
     5461 agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg ttgcccatta
     5521 tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt tttctaattt
     5581 tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg gcaaaaagta
     5641 ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc agtcaatcaa
     5701 tgtcatgaat gcaaaactag aatttaagta tgcaggaaca aaaacaaaag tagctttgag
     5761 tgaaccacag ccagaaattc aatcaaaaat gagatcgatg agagcagctg gtattgattt
     5821 aaacgataca tttctatcca tttacagttt aaatggacag tatcagcaac gtgtgtcttg
     5881 gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg
     5941 gtatgaaaaa atgggtagct taccttattt tgtcacaaca gatacagcat gtgcagaata
     6001 caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga
     6061 aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag
     6121 aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac
     6181 gaatagtatg atgtttagct ttgatactgc attgctatgg acaccacaac catcgaagcc
     6241 tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa
     6301 agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac
     6361 agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa
     6421 ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa
     6481 tggtacttgg aaaaaagctg atacaggagg aaagttaact gtatcaggta atacagtcac
     6541 ttatactgct tctgacagct ttttagctaa cgctgcaaat tacggtaggg attatatttt
     6601 gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt
     6661 aacgtttaac aatgatccga acaaaggaac acaaaaaaca aatacagtag aaaactattt
     6721 ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga
     6781 tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt
     6841 cacacaaaaa tggcatacaa aaggtgtaga cattgcctca gatcattaca agcaatttag
     6901 tattcaagat ccagtggaag atcgtttgac gtataaagaa ggttctgcgc gagtgataga
     6961 tatgagtaca ggaaaagata ttacttcaga aggtacacta acctatgata gcaattcaag
     7021 aacgctgaag tgggaagctt ccgctgattt cttaagtaaa aatcttttag atggacgaga
     7081 aattcagctg atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca
     7141 agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa
     7201 acctaactta cctcagatca ttgtgccaaa aactggatcg aatcatttag tgctaactgt
     7261 tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa
     7321 ataaatataa aaacaaggct gagaatcagc cttgttttct tttatttgaa gattggattc
     7381 gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga
     7441 cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc agggctattt
     7501 ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc
     7561 ccagtttgac ttatgttttc tcccttagca ccgtagcagc ctaagctttc caataaatct
     7621 tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga
     7681 atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg
     7741 cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat
     7801 gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt
     7861 tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga
     7921 tcagtcatgt ttttccaagg agtagcaaag ctatcccctt cgtatactgt ttttgattca
     7981 ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga
     8041 gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt
     8101 ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactg
     8161 tcaccgtgag tgacattgac tatctgttct ttcaaaatat taactcgtcc ttcgtttttg
     8221 tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt
     8281 gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta
     8341 tctttagcgc cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa
     8401 gttacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaata
     8461 gtaaccgtat attcttttgg tgtttcaggt ttgtgttgca accctttaaa tgcagcatca
     8521 agactatttt tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt
     8581 ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta
     8641 gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact
     8701 ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc
     8761 ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat
     8821 gcgttccagg tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga
     8881 gcttctagag ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata
     8941 ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg
     9001 tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt
     9061 ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat
     9121 ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga
     9181 tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat
     9241 tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta
     9301 tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga
     9361 ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt
     9421 ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggctaaccga
     9481 tatagtcaat aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt
     9541 tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc atagcattta
     9601 tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg
     9661 gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt
     9721 cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc
     9781 tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt
     9841 atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat
     9901 actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat ggtttgtctc
     9961 tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga aatcggaagc
    10021 taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag taggtggtct
    10081 tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa gtgtatggca
    10141 tccagatcct gtacaaataa cagttaagag gcaaaaagac tatgaaggtt tagaaagata
    10201 caaaatagta gattctgcag gcattaggga atttataaac aaacctgatt cattatttat
    10261 tgttgttccc tatgagaagg atggcgtaaa aaaaataatt tttcttggat ctagcaagga
    10321 tgagaagcga acgaaagaga aattaattca tgatggatca gagttaaata aatacaaatt
    10381 acaaaagact agtgagtgat tgctagtctt tttatttatc ctgaaggagg aatcacagtg
    10441 gcaaaagtta aaacaccatt cgaaaaagcc gcgtctaaaa tcttagaacg ccgtggtgtt
    10501 tctgaaatac agtggaaaaa agaagttctt gaaaaagcac aatttgacct attacgagga
    10561 aaagataagg acttagaaga atatatctat caacgtgaac gtgaaaaact tgtattcgat
    10621 gaaattatta aacattaaaa ggagcgaata atcttatgtt tgaatcaatt acaatgaatt
    10681 ttttaactca accgaaagta gtagctgcag ttgatatttt tagagcatta caaactgccg
    10741 caactagttt gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg gcttccattg
    10801 ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg aagacacacg
    10861 taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt gtagtttcaa
    10921 tgttctcaat gtttggacaa accccacaag gactataaaa aaaacaagta ggaggagtaa
    10981 aaagtacgtg gctacttttt actccttttt ttatgaaagg agaatattca tgttttttac
    11041 gatttcagga aatgcaaaat tggataaaga gttaataaga aaagcaacga agaaagtggg
    11101 aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa cggaagaaca
    11161 gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta aattgactct
    11221 cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg tttttggccc
    11281 actggattat caaatttcag aaggttttta tcctacattg atagatttaa tccgccttga
    11341 tgtgtttaaa gatacggatg aaactatgag tgtggaagag tcttatcgaa acgaaaaact
    11401 tttacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc aagtgagtga
    11461 agtcgaagta aatgaagaag taccatggga acgaccaatc tttccaaaga aggagtcaaa
    11521 agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga aatcgatatt
    11581 tgaggaagag aacgatgtga tgggtcaatt ggaggtagat ccctttgaag aaacaatgga
    11641 ggatgaagag acggacgaag tagaagttga tccagttgaa gctaatgaag agtcaacatg
    11701 gactgaaggg gattttcagg aatatattga accacttatc agtgaagaag tagaaaaaaa
    11761 cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat atccgccaga
    11821 gcatgaatgg atgaaagaaa aaaacaaatt atttttaaat gagctttatt cgaaatatca
    11881 attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct cacagggtaa
    11941 aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg agcttgcaca
    12001 agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag aaattaatga
    12061 gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa gaggaactaa ataaagaaga
    12121 acagcgagtg attgaagaag aagtacaaaa agtcaaagag cggtacgaaa agaaacgcga
    12181 caatgcttat tccttgtgtg tagaaaagat caacaatttt actaagacga atcaagaagc
    12241 gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa aagaagagtt
    12301 ctaccgcggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg aacaactgaa
    12361 taagctatat caaacaacga ctgaagttct tatggaaaaa cacgaacaga tccagcaaga
    12421 attacaagta caaattccaa aatggaaaaa agagtatgaa attcaacaac aagaattgaa
    12481 agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt gaacaattag aaataagaaa
    12541 acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggt tagagcaaga
    12601 aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata cattgcgtca
    12661 agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa cgccagtgaa
    12721 caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga tggtgggatg
    12781 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    12841 tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag aagcccagga
    12901 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    12961 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    13021 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    13081 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    13141 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    13201 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    13261 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    13321 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    13381 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    13441 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    13501 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    13561 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    13621 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    13681 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    13741 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    13801 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    13861 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    13921 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    13981 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    14041 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    14101 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    14161 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    14221 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    14281 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    14341 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    14401 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    14461 ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    14521 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    14581 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    14641 ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa cttattagta
    14701 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    14761 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    14821 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    14881 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    14941 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    15001 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    15061 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    15121 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    15181 gatcgtatct taactcctga aagaatttct acttttttag aaggagaaac gattgtactc
    15241 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    15301 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    15361 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    15421 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    15481 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    15541 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    15601 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    15661 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    15721 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    15781 aagtcttaca agattatggg aattatctgc agtatcaaga tgtatttagt tggatttgga
    15841 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    15901 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    15961 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    16021 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    16081 ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg ttaaatatca
    16141 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggatcacctg
    16201 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac gttactagca actgacccga
    16261 ataaagtaga atccctacca acagataaaa gaaatgcttt aacagcgaaa aattttaaaa
    16321 cagcgaatat caatgggatt attactcctg aacaagctaa aggtatggca ggtaatgata
    16381 aaagtgggtt atcagatata caaaaggatc ggttaaaggc attacaatac cgattggaac
    16441 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    16501 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatttta gctggtgaat
    16561 cttctttggc agtagcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    16621 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggtcaac
    16681 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    16741 tagaattacg aatctatacc ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    16801 agatcaatgg attcttgtat gtcgttgctt tgattgtact aaccattgcg ctattcaaag
    16861 gctctcaatc agttacgaaa attttcggcg ttgatacaag cctgaaaaac ggcagtaatt
    16921 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    16981 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    17041 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    17101 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    17161 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    17221 aagatcttac agaaaaagcc aaagacacct tagaagataa attagacggc acgagagcta
    17281 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    17341 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
    17401 tgaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    17461 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    17521 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    17581 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    17641 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    17701 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    17761 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    17821 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    17881 atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa tcacctgcag
    17941 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    18001 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    18061 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    18121 ttactgcacc aacaaaaata aatgtgacaa gagaaacaaa aaacaggaac aatgcattga
    18181 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    18241 gccttttttc tatgtatgaa aaggagaaat catttatgga taaatatagt aacatgaaaa
    18301 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    18361 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    18421 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    18481 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    18541 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    18601 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    18661 gaaaaccaga agagcgctct atcttggact tatttccata taaatcctgt acagaagagg
    18721 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    18781 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    18841 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    18901 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    18961 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    19021 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    19081 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    19141 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    19201 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    19261 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    19321 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    19381 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    19441 atcgggaaat agaaattgta aaatccaaag ggtatgattt agatttttta aaacaagtga
    19501 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    19561 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    19621 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    19681 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    19741 cgactgaccg aaataaaagc gcggaagaat acctggaact agcggaatat gctagaaaat
    19801 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    19861 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctcaaa gggatggatt
    19921 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    19981 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    20041 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    20101 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    20161 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    20221 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    20281 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    20341 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    20401 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    20461 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    20521 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    20581 ttacttcctc aaattcgcgt tcaaatactg atacgcaagt cacagggaaa gatccagaag
    20641 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    20701 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    20761 cgtatggcat gatttttgat ggtcatagta cgattcgaca ccttgaccaa caacagttgg
    20821 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    20881 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    20941 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    21001 agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa
    21061 aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct
    21121 tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg
    21181 gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctc
    21241 tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat
    21301 gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg
    21361 ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt
    21421 gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat
    21481 ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg
    21541 catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg
    21601 gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat
    21661 tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc
    21721 acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt
    21781 tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt
    21841 tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc
    21901 tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa
    21961 cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat
    22021 tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat
    22081 taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag
    22141 tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata
    22201 gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat
    22261 atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga
    22321 gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt
    22381 taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac
    22441 aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga
    22501 tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg
    22561 gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa
    22621 tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga
    22681 aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg
    22741 aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga
    22801 tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac
    22861 aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata
    22921 gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg attcataatt
    22981 acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac
    23041 caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt
    23101 acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa
    23161 tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata
    23221 aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga
    23281 agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg
    23341 acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc
    23401 ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata
    23461 gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa
    23521 gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac
    23581 tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac
    23641 gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta
    23701 cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt
    23761 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
    23821 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
    23881 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
    23941 atgcgtatgg atgaatctat tttacgtcat atagaaggat tagtgggtaa atcaatgacg
    24001 aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
    24061 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
    24121 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
    24181 caaagcggcg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
    24241 tcactgtgtt cgtgacccta tttctaatac ttgcggcggg ctcgattgat aattcggatt
    24301 cagattcaag cagtggtggc gaagctttta caggagaata ttcggaaggc ttaccgattt
    24361 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gccgtaggtg
    24421 cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt tacgaatcag
    24481 cgtttggtac atcggcgtcc gcaagaaatg atctcaacta ttttgggatt acttggtttg
    24541 atggttgtct gtttcctaaa ggaactgcac gaggaattgg tggcattgaa ggtggctggt
    24601 acatgaagtt tcccaactca aaagctgcat tttcctatta cggctttatg gtagccacac
    24661 aatccaattt taatgcttgt gtaggaaata aaagccctgg cgcaagctta ttgatcttag
    24721 gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat acaggttgta
    24781 tgtcaattat cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg
    24841 gtgaaggtgg caataacaac ggcacgatta ctggtgaatg gacgaatcca ttccctggca
    24901 gcagtttaga taaaagttcc ttcagcggtg gtcaattatt tggaacgaat ccaggcggtg
    24961 aattccgtcc gaacggcttc catgatggat tagattttgg ttcagttgat catccaggaa
    25021 gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt tggcaatcct ggtatttcag
    25081 gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt gaatatggtg tatcaagaat
    25141 ttgcaaatag tacaggaaat tctcgagtga aagtaggcga tcaagtgaaa gttggtcaag
    25201 taattgggat acgagataca gcacacttgc atttaggatt tactcgaatg gattggcgtc
    25261 aagcacaagg acatgcattc attgatgatg gtacatggat cgatccgtta ccatttttga
    25321 atagcagtaa aaaatagatc aggagaaaaa atatggaaaa taaaacgtta aaaatcgtct
    25381 taacagctgc aattagcgtt agtttaatcg gcaatattgc gttgttttgg cagctgaaag
    25441 aggaacaagc agcaaatacc tataaaattg aagaagtggc taaaaacgcg tctgagaagc
    25501 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
    25561 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
    25621 taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat
    25681 taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
    25741 aattaagtcc tacagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
    25801 taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg
    25861 tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca caaaaaataa
    25921 aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc atagacttta
    25981 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag acaacgtttc
    26041 aaaaaacgaa aaagttatct tagatggctg ggaatcttta tgattctcgg ctttttgatt
    26101 cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt aagcacgaac
    26161 gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc gaaccagcaa
    26221 ttagtttttg aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga actggctaat
    26281 cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat acgagccaaa
    26341 gtgattaagg tgtctgatga ttattttgca gtgaagctaa cgcatgtacc agaagaatat
    26401 atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa
    26461 ccgcaagatc tgatttttta tattcacgaa gataaggtca aagatcacgt gaaagacgaa
    26521 gattatgaac aacatgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca
    26581 gcacaaaaac aaattgaatc cttccaagca accattgatt tgaatgaaga attagtgaag
    26641 aaactaaaag atcaattgcc ttatcaagtc gcagaagatc aggaaaatac tgaaaataag
    26701 attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga tcaagaagag
    26761 agcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaatat ttagaagaat
    26821 aggtggagta aaagaaatga agcgagcgaa cttggtcggc aaagatactt tcgctgagca
    26881 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcaagaagt
    26941 gaattatcta acacaacaat atctgagttg aagaatggta gaaagagttt agatacctta
    27001 aaattcagat catttgaagc tctatatata gctgctaaaa aatattttac agctgataat
    27061 aaataaaaaa ttaaggtggg ttctatgtga aaaaaacgta tcaaaagttt ttatggatta
    27121 gctatatcgt cctatttatc gttgggtttc tcgtcagtgt gtccgtgatc gtcggaacgc
    27181 tttttctttt gttctctgac gaagatctgg tacttaaggg gttaatgagt acgttatcgt
    27241 gtgtcatgtt actttattta tgtatacaga tcaacgaaat tatgccccct cattggcaag
    27301 aaaagaagaa agaatttaag gaagaaatcg aaataaaatc caatgactgg attgaataca
    27361 catggcttcc tctgaaaaga aatagcaaac gaaaggaatt ttgggcattg caattatggc
    27421 caataggaaa actttttatt tatatggctt cacttatggt tactttaatc ggttatttgg
    27481 tggtggtcta tggggtggcc tattacttgt taagtgagcc aaaacagatc gcggcgtcaa
    27541 atattttcac ggcagtaatg acattaatgt gtatcatgtg cttttctatt tttagttact
    27601 tactcgtcca atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg
    27661 acttacttat cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa
    27721 aaacggtaga ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata
    27781 tgtatttgtt tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca
    27841 ttaaaagatc aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg
    27901 agtattacgt tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga
    27961 gtatccatga agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga
    28021 aaacaatcga ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat
    28081 atggcattgg gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt
    28141 taggaaaagt ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg
    28201 aaaatgattc ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag
    28261 aaggagcagt tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt
    28321 acgatgaaca ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
    28381 tcgattcgag agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat
    28441 gagtgggaac aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta
    28501 aagagcataa ggaaacgtga ccccactttt aaaaaaagtg gttggatagg ctgtgcctat
    28561 cacgtttctg aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata
    28621 gcaaaggata gtaaaaggat agaggtagga tagcgaaaag gataggatcc ttttcactat
    28681 cctatattat attggaggat atttatgaat gttaaaatta gagatttaga tccaaaattc
    28741 atttctgaaa ttgatcgaag atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt
    28801 cgtaatgatt atttaaagct actcgttgaa aatgattttg atcgtccttt gatggaatac
    28861 aaacaagaaa agtttgacca gttattagaa aagttttcag aaattcaaag ttataacaca
    28921 aagatattag aagaatatgt tagccaaaat aatcgtatta tagaaatctt gattgagcaa
    28981 aatagaggaa gtgaactttg atatggcaaa ggttatagac aaacacattt actttcacca
    29041 aacacatatc gatgtattgg atgaaactat ttctactcat gcagaggtta aaaactatag
    29101 tgaagctgtt cgctttttat gtatgaacta tgatccagaa gtggagaaaa aagtaaattc
    29161 caatcaagca aagttgaatg gaatgagtaa agaaatttct attttaaccg aaatggtttc
    29221 agaatttgcg gatcttcatt tgaaagatac aggaatatta attggtaaaa attcaactgt
    29281 ttatcaagaa gcaaaagaac gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata
    29341 ttcccctcag ttgaagaaac ctgtgaatga atctgttcat gccagagtag atccatttga
    29401 tccaacgaag attctaaaaa gataaaaatg aaggaggata ataatgactc aatcgccttc
    29461 agttattctc acttgtcaat ttactttgcc gaataaaaag tcattttcta cttatattga
    29521 ctatattaca agggaacagg ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca
    29581 ggaacttcaa cgaatcaaga atgctttaga ggagttttat attcctgaag gagaaacttt
    29641 ttcggagagt aagacgggta aagaattcag tgaaacgtcg aatgaagctc aatcgttgat
    29701 ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaatt atctttctta
    29761 tatgacacgt cactatgcgt tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga
    29821 ataccaacgc ttgaaccaag caatgaaaaa atatcaaaca gaaccccata aaaaagcttc
    29881 aggaaatctt cctggagttt tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa
    29941 ggagattaag caaatcttcc aaagaggaca acaaaaaggc tcaatcattt atcaagatgt
    30001 tgtttctcat gataattcgt atcttgaaaa aataggattg tacgatccca aaacagatac
    30061 tttagatgaa gaagggttaa aagcagctgg aagaaaaatg atggagacat tattcgagaa
    30121 agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaaacatat
    30181 ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga
    30241 aaatggtgtg agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat
    30301 gaagaccaat tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg
    30361 tatggagaaa cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt
    30421 ggtaaaagag ccagcgcgtt acgatcgaca cgcgatgaac ttattaaatg aagtatataa
    30481 aaccctacct gacaaacgtt cggaatggaa ttacggggat gaaaaacgaa ccaaactttc
    30541 accatacaca agagagaaac tcgatgagtt aaccaaatat gttttaacaa aagaacctga
    30601 atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg
    30661 ggaaagcaag agagaatcaa aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg
    30721 cacaggaaat gccatcctaa cagagctgaa gagacaggat agtcaaataa aaccattaat
    30781 agaaagtgtt gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca
    30841 attgccaacg aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt
    30901 ccaacaaaga gttgtcacgc agcggagttt gcgtcaatta aatcgaacga ttaatgccca
    30961 tgaagaaaaa taccacgcaa gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga
    31021 acaagaaaga cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg
    31081 aaagttatag aaggatcatt aagagaagaa cgaatcgaac aacttcctga ttgtttagaa
    31141 aaagagcatt tgttccagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa
    31201 tttttagtcg ttgagtaccc agctactggt gtgtattgtt gtaatcatgt gaatggtgaa
    31261 aaatatttta tcattcaaga atatgagggc aagttagcac cttattatac tacttgggaa
    31321 atgaatgaag agggaatcaa taactttcca tgtaaaagca
//
LOCUS       DACCPY010000023        31308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCPY010000023 DACCPY010000000
VERSION     DACCPY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.023"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVO69_000937"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVO69_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7399731.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVO69_000938"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVO69_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7399732.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IVO69_000939"
     CDS             complement(2738..3049)
                     /locus_tag="IVO69_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7399733.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IVO69_000940"
     CDS             complement(3046..3339)
                     /locus_tag="IVO69_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7399734.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVO69_000941"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVO69_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7399735.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVO69_000942"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVO69_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7399736.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IVO69_000943"
     CDS             complement(5177..9529)
                     /locus_tag="IVO69_000943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7399737.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IVO69_000944"
     CDS             complement(9741..11450)
                     /locus_tag="IVO69_000944"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7399738.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVO69_000945"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVO69_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7399739.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IVO69_000946"
     CDS             complement(12947..13747)
                     /locus_tag="IVO69_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7399740.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IVO69_000947"
     CDS             complement(13744..14556)
                     /locus_tag="IVO69_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7399741.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVO69_000948"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVO69_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7399742.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IVO69_000949"
     CDS             complement(15311..16033)
                     /locus_tag="IVO69_000949"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7399743.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IVO69_000950"
     CDS             complement(16169..17050)
                     /locus_tag="IVO69_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7399744.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVO69_000951"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVO69_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7399745.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IVO69_000952"
     CDS             18290..18769
                     /locus_tag="IVO69_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7399746.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IVO69_000953"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IVO69_000953"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7399747.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IVO69_000954"
     CDS             20070..20855
                     /locus_tag="IVO69_000954"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7399748.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IVO69_000955"
     CDS             complement(21201..22892)
                     /locus_tag="IVO69_000955"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7399749.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVO69_000956"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVO69_000956"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7399750.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVO69_000957"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVO69_000957"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7399751.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVO69_000958"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVO69_000958"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7399752.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27176..27877
                     /locus_tag="IVO69_000959"
     CDS             27176..27877
                     /locus_tag="IVO69_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7399753.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IVO69_000960"
     CDS             complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IVO69_000960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7399754.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IVO69_000961"
     CDS             complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IVO69_000961"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7399755.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IVO69_000962"
     CDS             complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IVO69_000962"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7399756.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IVO69_000963"
     CDS             complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IVO69_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7399757.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         8491 a         6716 c         5523 g        10578 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    26881 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa aattttttga ataaattaaa
    26941 aaaataaatt atattagcgt tgttagcgtt tactatatgt attattgttg ttttttatac
    27001 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
    27061 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
    27121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
    27181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
    27241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
    27301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
    27361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
    27421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
    27481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
    27541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
    27601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
    27661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
    27721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
    27781 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
    27841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
    27901 cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
    27961 gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
    28021 cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc
    28081 tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg
    28141 gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat
    28201 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
    28261 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
    28321 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
    28381 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca
    28441 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
    28501 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
    28561 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc
    28621 aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact
    28681 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
    28741 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
    28801 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
    28861 atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
    28921 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
    28981 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
    29041 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
    29101 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
    29161 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
    29221 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
    29281 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca
    29341 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
    29401 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
    29461 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
    29521 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
    29581 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
    29641 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
    29701 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
    29761 tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
    29821 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
    29881 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
    29941 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
    30001 tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca
    30061 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
    30121 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
    30181 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
    30241 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
    30301 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
    30361 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat
    30421 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
    30481 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag
    30541 cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca
    30601 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
    30661 tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
    30721 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt
    30781 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
    30841 aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta
    30901 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
    30961 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
    31021 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
    31081 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
    31141 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
    31201 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
    31261 gcaaaactag tacaatgata catgattatc tgctggcggg caaatttt
//
LOCUS       DACCPY010000024        30481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCPY010000024 DACCPY010000000
VERSION     DACCPY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.024"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVO69_000964"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVO69_000964"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7399758.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="IVO69_000965"
     CDS             complement(2423..3118)
                     /locus_tag="IVO69_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7399759.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IVO69_000966"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IVO69_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7399760.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="IVO69_000967"
     CDS             complement(4317..4508)
                     /locus_tag="IVO69_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7399761.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="IVO69_000968"
     CDS             complement(4529..5419)
                     /locus_tag="IVO69_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7399762.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="IVO69_000969"
     CDS             complement(5406..6173)
                     /locus_tag="IVO69_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7399763.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IVO69_000970"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IVO69_000970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7399764.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IVO69_000971"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IVO69_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7399765.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IVO69_000972"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IVO69_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7399766.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10789..11127
                     /locus_tag="IVO69_000973"
     CDS             10789..11127
                     /locus_tag="IVO69_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399767.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11295..12053)
                     /locus_tag="IVO69_000974"
     CDS             complement(11295..12053)
                     /locus_tag="IVO69_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7399768.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="IVO69_000975"
     CDS             complement(12071..12889)
                     /locus_tag="IVO69_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7399769.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IVO69_000976"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IVO69_000976"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7399770.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IVO69_000977"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IVO69_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7399771.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14017..15351
                     /gene="dnaA"
                     /locus_tag="IVO69_000978"
     CDS             14017..15351
                     /gene="dnaA"
                     /locus_tag="IVO69_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7399772.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15549..16679
                     /gene="dnaN"
                     /locus_tag="IVO69_000979"
     CDS             15549..16679
                     /gene="dnaN"
                     /locus_tag="IVO69_000979"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7399773.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16908..17150
                     /gene="yaaA"
                     /locus_tag="IVO69_000980"
     CDS             16908..17150
                     /gene="yaaA"
                     /locus_tag="IVO69_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7399774.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            17137..18261
                     /gene="recF"
                     /locus_tag="IVO69_000981"
     CDS             17137..18261
                     /gene="recF"
                     /locus_tag="IVO69_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7399775.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            18258..20204
                     /gene="gyrB"
                     /locus_tag="IVO69_000982"
     CDS             18258..20204
                     /gene="gyrB"
                     /locus_tag="IVO69_000982"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7399776.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            20224..22695
                     /gene="gyrA"
                     /locus_tag="IVO69_000983"
     CDS             20224..22695
                     /gene="gyrA"
                     /locus_tag="IVO69_000983"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7399777.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22931..23230
                     /locus_tag="IVO69_000984"
     CDS             22931..23230
                     /locus_tag="IVO69_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7399778.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            23280..23792
                     /gene="ssb"
                     /locus_tag="IVO69_000985"
     CDS             23280..23792
                     /gene="ssb"
                     /locus_tag="IVO69_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7399779.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23818..24054
                     /gene="rpsR"
                     /locus_tag="IVO69_000986"
     CDS             23818..24054
                     /gene="rpsR"
                     /locus_tag="IVO69_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7399780.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            24197..26170
                     /locus_tag="IVO69_000987"
     CDS             24197..26170
                     /locus_tag="IVO69_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7399781.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            26176..26628
                     /gene="rplI"
                     /locus_tag="IVO69_000988"
     CDS             26176..26628
                     /gene="rplI"
                     /locus_tag="IVO69_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7399782.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26902..28269
                     /gene="dnaB"
                     /locus_tag="IVO69_000989"
     CDS             26902..28269
                     /gene="dnaB"
                     /locus_tag="IVO69_000989"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7399783.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            29049..30341
                     /locus_tag="IVO69_000990"
     CDS             29049..30341
                     /locus_tag="IVO69_000990"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7399784.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9205 a         6018 c         6019 g         9239 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca acactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaaaggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc
    13801 aagaggaaat ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat
    13921 taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac
    13981 tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg
    14041 aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc
    14101 gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag
    14161 attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg
    14221 ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa
    14281 gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg
    14341 aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca
    14401 gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga
    14461 ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt
    14521 cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat
    14581 tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg
    14641 ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat
    14701 actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca
    14761 aacgagattc cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt
    14821 gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct
    14881 gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac
    14941 attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa
    15001 gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat
    15061 cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg
    15121 aaagatctca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg
    15181 tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc
    15241 aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt
    15301 gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat
    15361 tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact
    15421 ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac
    15481 atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga
    15541 gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc
    15601 aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga
    15661 cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt
    15721 ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag
    15781 cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt
    15841 tagaaaataa acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag
    15901 atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg
    15961 tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc
    16021 gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta
    16081 ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg
    16141 atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag
    16201 aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt
    16261 tctattcccg tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt
    16321 ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt
    16381 tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg
    16441 ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa
    16501 gcagtggcga tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg
    16561 catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg
    16621 aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac
    16681 aatgattttc aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa
    16741 acagaaggcc ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt
    16801 ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa
    16861 aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag
    16921 tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg
    16981 gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac
    17041 cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag
    17101 gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta
    17161 aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt
    17221 ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg
    17281 acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg
    17341 atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct
    17401 aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg
    17461 ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg
    17521 cagaccaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat
    17581 gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat
    17641 gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt
    17701 ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat
    17761 tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca
    17821 gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg
    17881 aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac
    17941 gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa
    18001 agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg
    18061 gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc
    18121 catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac
    18181 catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta
    18241 aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg
    18301 atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt
    18361 atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt
    18421 cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca
    18481 acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg
    18541 gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg
    18601 gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgtt aacgcgcttt
    18661 ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc
    18721 gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg
    18781 ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat
    18841 tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag
    18901 acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg attaaaagtt
    18961 atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag
    19021 gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca
    19081 atatcatgag ttttgccaac aatatccata cgtatgaagg cggaacacat gaatcaggat
    19141 tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag
    19201 aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta
    19261 tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag
    19321 tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc
    19381 caagtgtggg ccgtcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag
    19441 cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag
    19501 gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag
    19561 gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc
    19621 ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg
    19681 aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat
    19741 cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata
    19801 tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat
    19861 acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg
    19921 tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct
    19981 caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat
    20041 gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta
    20101 ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat
    20161 tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa
    20221 agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa
    20281 gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga
    20341 gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca
    20401 ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat
    20461 cctcatggag actatgcgat ctatgagtca atggtacgga tggcacagcc attcagctac
    20521 cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc
    20581 gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc
    20641 aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta
    20701 ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg
    20761 gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg
    20821 gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca
    20881 acaggtggtt tggttatggg aaaatcagga atccgaagag cgtatgaaac aggtaagggt
    20941 tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc
    21001 atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa
    21061 ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa
    21121 ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catctgttat cttgaataac
    21181 ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa
    21241 ggtgtgccga aagttttgag cttgaaacaa attcttgaaa actatatcga acatcagcgc
    21301 gaagtcatca caagacggac agtctttgaa aaagaaaaag cagaagcaag ggcacacatt
    21361 ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg
    21421 tctaaatcag atgacgaagc aaaagcgacg atgatcgaac ggttcgattt atccgatcgc
    21481 cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa
    21541 atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca
    21601 agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt
    21661 ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat
    21721 ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg
    21781 gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc
    21841 cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc
    21901 tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgc
    21961 acagccaaag gaataccaat tatcaattta ctaggtatcg attcgagtga aagcatccag
    22021 gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt
    22081 caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg
    22141 atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa
    22201 tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc
    22261 cgcgatatgg gacgtacagc ttccggggtt agaggaatac gcctgcgtga ggaagattat
    22321 gtcgttgggg cagccttaat gaaggaagat caagaagtat tagtgatcac agaaaaaggt
    22381 tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc
    22441 aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga
    22501 gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact
    22561 gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg
    22621 aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa
    22681 ggatcagaag aatagccatc aagactaatt gtgtaagcta gcaagaatga ccgattaaaa
    22741 aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac
    22801 tgttcattct gtattgcttt tgaccaaaat agagtgtata atcagagctt gtgagtagtc
    22861 taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag
    22921 gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga
    22981 tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc
    23041 agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta
    23101 ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa
    23161 tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga
    23221 agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt
    23281 tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag
    23341 caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa
    23401 gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt
    23461 tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt
    23521 cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc
    23581 aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt
    23641 ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat
    23701 ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga
    23761 tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg
    23821 gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat
    23881 attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt
    23941 aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt
    24001 gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc
    24061 ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa
    24121 taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat
    24181 atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct
    24241 tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag
    24301 cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg
    24361 ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa
    24421 tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca
    24481 aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga
    24541 ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct
    24601 agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga
    24661 tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat
    24721 tttatctatt gataactatg cagatgccat tgataaattg gatgacaaag agatttctta
    24781 tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt
    24841 caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat
    24901 gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc
    24961 gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg
    25021 agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa
    25081 agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa
    25141 acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac
    25201 aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt
    25261 tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaacgagaa
    25321 cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga
    25381 aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt
    25441 gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga
    25501 taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct
    25561 gacttatatc gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata
    25621 ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat
    25681 ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag
    25741 ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt
    25801 agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat
    25861 tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc
    25921 agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga
    25981 cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga
    26041 atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt
    26101 tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga
    26161 taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa
    26221 ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg
    26281 gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa
    26341 aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa
    26401 gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc
    26461 ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga
    26521 aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt
    26581 catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata
    26641 gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag
    26701 aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc
    26761 ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt
    26821 ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat
    26881 tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat
    26941 attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat
    27001 gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag
    27061 acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg
    27121 ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca
    27181 ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca
    27241 ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga
    27301 gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa
    27361 agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc
    27421 gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg
    27481 gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg
    27541 gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt
    27601 tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt
    27661 gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg
    27721 tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca
    27781 ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc
    27841 aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac
    27901 cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa
    27961 gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga
    28021 ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc
    28081 ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag
    28141 ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga
    28201 acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa
    28261 gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc
    28321 aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt
    28381 gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt
    28441 ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata
    28501 ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact
    28561 cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt
    28621 gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg
    28681 catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt
    28741 tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt
    28801 agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt
    28861 ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta
    28921 aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt
    28981 tatttgaaat atgtatctat tattgataca atggttcagg attaaataat atagaacgag
    29041 gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa
    29101 aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca
    29161 atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg
    29221 ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat
    29281 cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta
    29341 tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag
    29401 atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg
    29461 ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag
    29521 aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag
    29581 aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac
    29641 aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt
    29701 tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta
    29761 cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa
    29821 tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc
    29881 caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta
    29941 cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta
    30001 aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg
    30061 aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag
    30121 caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag
    30181 aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac
    30241 gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa
    30301 cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga
    30361 atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga
    30421 acactgttta ttcagaataa gggactgtga caaaaaccgt gtcacagtcc ctttttccga
    30481 a
//
LOCUS       DACCPY010000025        30409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCPY010000025 DACCPY010000000
VERSION     DACCPY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.025"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IVO69_000991"
     CDS             complement(1273..1938)
                     /locus_tag="IVO69_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399785.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVO69_000992"
     CDS             2064..4100
                     /locus_tag="IVO69_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7399786.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVO69_000993"
     CDS             4104..4742
                     /locus_tag="IVO69_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399787.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVO69_000994"
     CDS             4770..5198
                     /locus_tag="IVO69_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399788.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVO69_000995"
     CDS             5655..6566
                     /locus_tag="IVO69_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7399789.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IVO69_000996"
     CDS             6783..7220
                     /locus_tag="IVO69_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399790.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IVO69_000997"
     CDS             7315..8199
                     /locus_tag="IVO69_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7399791.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IVO69_000998"
     CDS             8423..11590
                     /locus_tag="IVO69_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399792.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IVO69_000999"
     CDS             11610..12155
                     /locus_tag="IVO69_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7399793.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IVO69_001000"
     CDS             12183..12638
                     /locus_tag="IVO69_001000"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7399794.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IVO69_001001"
     CDS             12635..13393
                     /locus_tag="IVO69_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7399795.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IVO69_001002"
     CDS             complement(13479..14219)
                     /locus_tag="IVO69_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7399796.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IVO69_001003"
     CDS             14280..15533
                     /locus_tag="IVO69_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7399797.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IVO69_001004"
     CDS             15855..16082
                     /locus_tag="IVO69_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7399798.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IVO69_001005"
     CDS             16205..16531
                     /locus_tag="IVO69_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7399799.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IVO69_001006"
     CDS             16599..17312
                     /locus_tag="IVO69_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7399800.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17615..18466
                     /locus_tag="IVO69_001007"
     CDS             17615..18466
                     /locus_tag="IVO69_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7399801.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18587..20452
                     /locus_tag="IVO69_001008"
     CDS             18587..20452
                     /locus_tag="IVO69_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7399802.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20500..21909
                     /locus_tag="IVO69_001009"
     CDS             20500..21909
                     /locus_tag="IVO69_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7399803.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22017..22718
                     /locus_tag="IVO69_001010"
     CDS             22017..22718
                     /locus_tag="IVO69_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7399804.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22877..23356
                     /locus_tag="IVO69_001011"
                     /pseudo
     CDS             22877..23356
                     /locus_tag="IVO69_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24204..24779
                     /locus_tag="IVO69_001012"
     CDS             24204..24779
                     /locus_tag="IVO69_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7399805.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24779..25219
                     /locus_tag="IVO69_001013"
     CDS             24779..25219
                     /locus_tag="IVO69_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7399806.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25235..25936
                     /locus_tag="IVO69_001014"
     CDS             25235..25936
                     /locus_tag="IVO69_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7399807.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26424..26849)
                     /locus_tag="IVO69_001015"
     CDS             complement(26424..26849)
                     /locus_tag="IVO69_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399808.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            27223..28194
                     /locus_tag="IVO69_001016"
     CDS             27223..28194
                     /locus_tag="IVO69_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7399809.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            28226..29623
                     /locus_tag="IVO69_001017"
     CDS             28226..29623
                     /locus_tag="IVO69_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7399810.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            29782..30405
                     /locus_tag="IVO69_001018"
     CDS             29782..30405
                     /locus_tag="IVO69_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399811.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        10322 a         4936 c         6264 g         8887 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tatatgaaaa ggcttgcata aataaaaatt cgatggtatg atgattttga ataagaaatg
    17521 gattgttact gtacggcagg caaaaccaga attcagctaa gaaacagtag gcgaattcta
    17581 ggttttttta tttttcttga gagggcgaag caaaatgaaa attgataaaa ttttgaataa
    17641 caatgtcgtc atttcgaaaa atggattcgg ggaagaagtc gtttgtatgg gaaaaggact
    17701 agcttttcaa aaaaagattg gggacgagat ttcgccagaa gcggtccaaa aagaatttgt
    17761 tttaagagat tcttttgcta aaaaccaatt ccagcagctg atggctgata ttccggcaga
    17821 ggagatcgaa tgggtcaaac aagtagtcga actggctgaa gataaactaa aagttgaatt
    17881 ttcgcctaat atttatctga cattgaccga tcatcttcat tatgcgatca cacgagcaaa
    17941 agaaaatatc caactgccta atccattatt atttgaaaca aaaaaatttt atccgaaaga
    18001 atatcaagca gcgaaagagg ctcttttgtt cgtgaaacaa aagacgggtg tggaactttc
    18061 tgaggatgaa gccggtttta ttgcctttca ttttgtgaat agccaacaag gaaacgaaga
    18121 catgcaagtg acgatgaccg ctaccacaat ggtaagagat atactgagta tcatcagtaa
    18181 attcttcggc atcgtgttcg atgaagaatc tttgaattat cagcgtatca ttactcatct
    18241 gcagtttttt gcccagcgtt atctcaaagg ggaaggatct gacgagcaag atgaatttct
    18301 ctacgcatta gttcaaggaa agtatccaaa agcgtttcat tgtgtcgaac ggatcaatga
    18361 gtatcttcga caaacgcaaa aacaggaaat ggggatcgca gagcagattt atttaacgat
    18421 tcatattcaa agagtagtaa gtgagaaaaa aagcatccaa tcataaggat tgttactgta
    18481 tggcaggcaa aacctggatt gaaaaaatag gttcacgaat gtttgtgact gtttttttca
    18541 atctgggttt ttatttttta aataaaaaag aaagaaggaa aaaacaatgg ataatcaagc
    18601 agttggtcgt cgtgtatggg aagcggtagg aggacaaaag aatgtcaaaa gtttagtaca
    18661 ttgtgctacg cggttgcgat tcagattaaa agatgaatct ttagcagata cgcaaaagtt
    18721 aaaagaagac ccggacgtga tccaagtcgt acaaagtggc ggtcagtatc aagtagtgat
    18781 tggcagtaat gtagcagatg tttatcaatc gatcgtggat gaggaaggtc ttgcagtttc
    18841 ggaggatgga aaagaagagt ccaaaaatat tttgaatcgt ctgatcgata ttatctctag
    18901 tatatttaca ccttttctag gggcgatggc agccgccggg gtattgaaag gattcttatc
    18961 attggcaacg gtgatgggct ggctgacagc tgatagcggt acttatcaaa tattgtttgc
    19021 tgcagcagat ggtgtcttta ccttcttacc tgtcatgctt gcttttacag cagctaaaaa
    19081 gttcaaagcc aaccagtttt tagcagtagc aattgctatg gcacttgtgt atccagctat
    19141 cacagcagtt gctggtgcgg gagaagctat cagtttcttt gggatcccag tgattctttc
    19201 accatctggt tatacttctt ctgtcatacc aatcattcta gccgtatggg ttcaaagtaa
    19261 attagagcca ttcgtaaaaa aagtgattcc gcaatttctg caaatgattt tagttccgct
    19321 tgtcgtttta gtagtgatgg ttccattaac atttcttgct ttaggaccaa tcgggacggt
    19381 tgccggtaat gctttaggcg gtctcttcaa ctcgatttat ggatttagtc caatcgtagc
    19441 aggtctaatc atgggaagtt tatggcaagt attcgtgatg ttcggcatgc attggggatt
    19501 cgtaccgatc atgtttttga atatcgaaca atatgggttt gatgtattga tgccaatgct
    19561 tcttccagcc atcttggcac aaggtggggc tgctttagct gtagcattgc gaacaaaaga
    19621 tacgaaactt cgagcattag ggatttcttc tacagtgact tcgttatttg gaatcactga
    19681 accaactgtg tatggtgtga cattaccttt gaagaaacca ttcattgccg cttgtatttc
    19741 tggaggtatt ggtggagcta ttatcggatt ttccggagta aaagctttct ccagcagttt
    19801 ggtcagctta ttgacgattc cgactttcat taatacggtg gatggtgtag agtcaaatgt
    19861 gacagtagca gtaatagcta caggaattgc ttttgttctt gcattcgtag gtacattgat
    19921 tttaggattt gacgaacaaa cacaagacaa tcaattagaa aacaaacatg cgaatgcagg
    19981 agaaccgatt acttcagcac ggcatacctt aaaaagtcct ttaacgggta aagtactgcc
    20041 attgagtgaa gtaccagacc aagttttttc ttctggcgtg atgggaaaag gtatcgctat
    20101 tgatccagaa gtaggagaac tagttgcacc tgcagacgga gagatcacga ctatttttcc
    20161 aactggacat gctgtaggga tcacaacgac tgacggagcg gagattttga ttcatatcgg
    20221 gatggataca gtagaattaa atgggaatgg ctttgagatc ttagtcaaac aaggggatct
    20281 cgtcaaagca ggagatttat tgattcgttt tgacatcgaa gcaattagag cagctggcta
    20341 tagcgtaatc actccagttg tcattacgaa tactgatgca tttgccgata tacttgaatt
    20401 agaccaaaaa gagattattg caaacgaaga tgttctggca attgtaaaat aaggaggaaa
    20461 tcaggatgca atcacgtttt tcagaaaaat ttttatgggg tggagcaact gctgccaaat
    20521 caattagaag gggcttatga tcgagacgga aaaggactat ccgttgcaga tgctatgcct
    20581 ggaggtaagc agcgctttgc gattatcggc agtgaagaat ttgactggac gattgatcaa
    20641 gaaaaatata tttacccgaa tcatcgagga atcgatcatt acgatcgttt caaagaagat
    20701 cttgccttgt ttgcggaaat gggattcaaa tgttatcgtt tttcgattgc ctggacacga
    20761 atttttccaa atggagacga aggtactcct aacgaagcgg gattagaatt ttatgatcag
    20821 ctgattgatg agtgcttaaa atatgatatt gaacctgtga tcacgatttc tcattacgaa
    20881 atgcctcttc atttggcaaa agaatacggt ggctggaaaa atcgaaaact gatcgacttc
    20941 tatgaacgat ttgctcagac agtattagag agatacagca gtaaagtcaa atattggatg
    21001 actttcaatg aaatcaattc ggcctttcac tttccagctt taagtcaagg attagtaaaa
    21061 agcaatggag caggagagta ccaaaatatt ttccaagcat ggcataatca attcgttgca
    21121 agtagcaaag ccgtaaaaat cggccacgaa ttgcgttcgg atatccaaat cggctgcatg
    21181 attatctatg caacaactta cagcatcgat gccaatccag tcaatcaggc agcaacaatg
    21241 atccaaaacc aagaattcaa ttttttctgc acggatgtcc aagtacgtgg agaataccct
    21301 gcatacacag cgcgtaccca taaaaaatat ggagtcgatc cggaaaaatt agagcaaacg
    21361 gaaaaagact tccagttgct aaaagaatat ccagtggact acatcggatt tagttattat
    21421 atgtctacgg caatcaatga gactgatcca caagcagcaa cttcagaagg gaacttgctg
    21481 ggaggagtaa aaaatccgtt tcttgaggca agtgaatggg gctggcagat cgatccagaa
    21541 gggctgagaa tcgcattgaa tgaattatac aatcgttacc agaaaccatt attcattgtt
    21601 gaaaacggtc ttggagccat tgatcatatg gaagaggatg gcacgattat tgatgattat
    21661 cggatcgatt atttacgtcg tcatatcgaa gcgatggcta atgcagtagc agacggtgta
    21721 gatttgatgg gctatacgcc ttggggatgt atcgatcttg tcagtgcatc aacaggagaa
    21781 atgagtaaac gttacggctt tatctatgtg gatctagacg atgaaggaaa tgggacactg
    21841 aatcgatcaa agaaaaaatc ttttgattgg tataaagaag taattgcctc aaatggcgta
    21901 aacttgtaat taaagcagct gaatgaaaaa gaacacactt ctcacatgtg atttgtgttc
    21961 tttttttatt ttttatgtgc tattctttta gtaatagtaa agcaaagggt gacgttatga
    22021 aaaggacacg tgtactatac ttggaagtcg cagataaaat taaagaagat attttctcag
    22081 gaaaatatcc agtcggttcg atgttgccga cagaaagtga attagaagaa ctgtttcaag
    22141 tcagcaaaat cactgtccga aaggcaatcg aactgctggc atcagatgaa tatgtcgcaa
    22201 agaaaagtgg tcgtgggacg acggttctaa gcaatcgtcc ttacaatcga ttatccaaag
    22261 cggcaagctt tacgcaaatc ttggagaact cccgatatga agtccggaaa gaaacgctgg
    22321 atttgcaatt ggtccatata aagaaggaag atcctttgtt ttcctttttc ggtgagcaag
    22381 ctgtttgttt taagcgctta tattatttga acaatcagcc ttatatttat tttgttcatt
    22441 atcttccaca agaaatgatg caattcagca gagaagaatt tggcaaagaa tctttgtatc
    22501 gtttgctgac gaaaaaagga tatactattg actcattttc tgatgatttc tcggcagtat
    22561 ttttaacaga agaagaacaa ggaattctga aaacagacgc acagatcggc atcaaacgga
    22621 tacggaaatc gtctacgcaa attggggaaa tgattgaata ttcggaggcg gtctaccata
    22681 cggagctgca tccttaccat attgattatg aaacataaat aaaaaaagag gttgtgaaaa
    22741 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcgt
    22801 ttggttaggc ttattaccgc agatacacgg ctgtttctgg attaccattt attctggata
    22861 agggggtgtg gcacgatttt cagaaggaat ctatgaacca atcactgtat tgattttagc
    22921 ttcgatggta accccattcg gcttgacgat cgcttatttc ttagggaaaa tcattcgtaa
    22981 aaatatactc aatcgtcaag aaatcgatac attgaagacc gcttttccta tggggatctg
    23041 tcagatcaca gagggatgtt tccctatcgt attgaacgac ttggcacgca atgtcatagc
    23101 aacgggagtc ggtgggacag taggtggcgg attaagcatg ttttggggcg cagatagccg
    23161 tattccggca tcgggaatgt ttgcagtcgc tacgatgacc agaccatggg cattcattgg
    23221 tgcattgtta gctggttctt ttgtcacagg aataaccatt ttattattga aaaaaccagt
    23281 agatccgaat gcagaaatca tccaagaaga aaaagaggaa gaagacatat cttgggatga
    23341 tttgacaatt agttaggaga ggcagaatgg attacaagaa aaaagtacaa tagatgtacc
    23401 ttgagacagg aatggtatga aagaatcaga gttagaaaga atcgaatata cagattttgg
    23461 tttaggagcg atcgaaactg tccagagcat cggcaccctc cttgaaatga cgatgaagga
    23521 aaaatgagaa cctttcgttg tcgaaagggt ctggtatacc ttatgtcgaa ggaaaggaaa
    23581 caaaaaaact ttcctgtact attccaaaaa ggaaagaaag ttattataca gtaaaaaaag
    23641 aaattattct tcatgctaga gatcagtaca cgttcgagcc caatatcaaa cactagttcc
    23701 aggcaggcgc aacaggtgcg gttatcactg aattttcttc cagcagtgac gattcaagtg
    23761 atatttttac aaatccagct atttatcgtt aacaaaaaga tttttaggag aagctgatga
    23821 acaaaaatga tcaaattttg atcgtttagt tcgtcggctt ttttatttag agaaacatca
    23881 tttatcagtg aaacataaca cgagaacaaa atgatttttt cgttaataaa agaaacaagt
    23941 atttgttgtt taaaataata cacgttattg gcatatagga cgaaatccca gtatcttcta
    24001 taagtaagtc atttcatatc gatgaaaaca cgttatttat ttttgtttta tctataaaat
    24061 ggctgaaaaa tgattttttt ctaaatacta tatatagtgg cgtctatcct atataccact
    24121 aaaaaactac aaaatctagt cttttatttt aaaaaaacac gtgtagaatg aagatagact
    24181 tttgagttag ggagtgtttt tttatgaaac ttactgtttt ttgcggttcc cgttttggta
    24241 ataaagaaag ttacaaattt attgcgcaaa cgttaggaaa atacatggca caagaagaaa
    24301 tcgaactggt ttacggtggt tcatactccg ggattatggg agtgattagt aaaacagtac
    24361 tggaaaataa cggaaaagtc acaggtatct atccgaatgg gctttttgaa actgaattgc
    24421 ctggaaaaga tgttactaca tttattccta cagaaacgat cgatgaaaga aaggtattat
    24481 tatttgaaaa gggagatgct gtcctcgttt tccctggagg tctaggtaca ttggaggaat
    24541 tctcacaact tctttcttgg atagcaatcg gtctgacgcc cgacaagcca atcggaatat
    24601 tgaatattgg cggttattac agcggcttgc aacaattatt agaaactttt gcaaaagagg
    24661 attttatgga taaaaaatgg ttggatcaag taatcttttc caacaatcca ttggaactgg
    24721 tcaatgaatt acgagcagta tctattaaaa atcagttatt attgaaggag gcaaaataat
    24781 gagtcaaata tatttagcag gaccattttt ttcagaggag caaatcgatc gagtaagtcg
    24841 tatcgaaaaa gcgttggagg aaaacaaaac agtcacaagt ttctactctc cacgtcatca
    24901 ccaagaaagc aattatgaac tattcagcgc tggatgggcg caggaagtat acgaaaaaga
    24961 tatggaagaa ctgacaaatg cagaatttgt tgttgctatc cttgactttg agcatcaaac
    25021 aatcgatcca ggaacggctt acgaattagg tgtggcaact atgttgaaaa aaccgatgat
    25081 cattgtacaa gaagagacgg tacctacgaa cttgatgatt acacaaagtc tccatacgta
    25141 tttaaaatca gaccaagctg tacgtgaata tgattttgaa acattgcctg ttgaaactta
    25201 cgtaggcgag tacttataga agaggaaggg ttgtatgtca gtgattttga tttcaggaac
    25261 gattggtgca ggtaagagca gtttgacgga tatgcttgca aaagagattg attctaaacc
    25321 attttatgaa aatgtcgaag ataacgaagt attgccgctc ttttattcaa atcctgagca
    25381 gtacgctttt ttattgcaaa tcttcttttt gaacaagcga tttttagcaa tgaaaaatgc
    25441 tttagtgaac gatgacaatg tgctagaccg atccatctat gaagatagcc tgttgttcca
    25501 tctaaacgct gatttaggtc gggtaacgga tatcgaagta cagcagtacg atagcttatt
    25561 agatacgatg ctaaacgagt tagatgatgt tgctccaaaa aaacggcctg atttgatggt
    25621 ccatatcaaa gtttctttgg acacgatgct agaacgtatc aaaaaacgtg ggcgtgacta
    25681 tgaacagcta gaaagcgatg agacgttata tacctattat gaaacgttga acacccgcta
    25741 taatcaatgg tatgaagatt ttgatgtatg tcctaaaata caagtggatg gggataaatt
    25801 cgattttgta gaaaatccag aagatgcaaa aaaggtgatc cgtcagatcc aagaaaaatt
    25861 agctgaattg gaaggaaagc ctgctggaaa atattttacg gataaccagc atgcaaaagg
    25921 tcaagaatta ggctaaaaat aaaaaaccaa acgacgggaa agaaaaatta ctacgtgatg
    25981 tagaataagc ctttctatcg tttggttatt ttatattcaa atatttgaaa taaataaatc
    26041 tttactcttt gatttgtaaa ctgaaatgat ccactgtttg ttttactgat gaagcttgat
    26101 caaaggaaga agctgagttt gaagttcata aaaatcagcc atagtgagtt ttgcttggat
    26161 ttgtttatct tcttctactt tttacataat tttgaacagg ataagattca aatgttttaa
    26221 atgaatcttt gactagtttg tgacagttgt cgcatatgct caagcttcct tggtcattaa
    26281 attctgtatg gatatttttg ttgttattac atgtacaata atcaggacta tcgatcatcc
    26341 atttcgtctc ctttacaatc atcttgcttc tttactatag cagaaaagaa ggggagaaac
    26401 aaaagaagat ccaacagacg gcactataga ctgtagatgt tgccatcctc atccttcaaa
    26461 cggtaattac cgatttttgt aaatatgact gttgtttctt ttttattttc ttttcctgaa
    26521 ataatatatt cgtcatcttt ggtagtgatc ttgatatctg tcagcactgt tttttcttca
    26581 gatgattcag aagaatcagt tccttgtgtc tgatacgtgc gtgtccattc cattttttta
    26641 tctgtaaatg tcaactgtcc cgcggataaa tccatatctt tacgtacttt tagcgtatat
    26701 acactgttgg tggtatctgc tttttccgct tttaagacag aagaatcggt tgtttcgtcc
    26761 gaggattggt cagagccgcc aaacaaatca cagccagtca atgtcaaaag tcccgcagac
    26821 aaaataaata gtacgattaa tttcttcaaa atacttcccc catttgatag gataaagaaa
    26881 ttataccatt ggaatgtaaa acttcaaacg gatggaagag aaaaacgcac aaaacaccca
    26941 caaatagagt aaattttaga ataaaatttt aaaaaggcaa aaaatccgcc actttcagaa
    27001 agcgttttaa aaaagtgttg caatctttta aaaaaatgct atattaaaaa tgaaccggtt
    27061 caaatacttg tttaagtata attaatagtg agaggaagtt gctctactga agactttagt
    27121 tatatggata ttgatgacaa gcaacactat gcaaagaatg cggtcatcat aatcgaacaa
    27181 caagaaagag aaatcaaatg taactggcag gagtgagaat gtatggcatc gatccgtgat
    27241 gtggctaaga tggctggtgt ttcagtggga acagtttccc gttacttgaa tggtcagcaa
    27301 ctgaaggaaa agaacatgaa aaaaatagca gaagcgattt cagcgttgga ctataaagaa
    27361 aacatcattg ctaaagggtt gaagaataat cgaagcttct ctattggact tttgataaac
    27421 gggatttcca gccgttttgg gtcggaagtt gtctcaggga tcgaaagagt agctgaagaa
    27481 aatgggtata gcttattatt aagcgggttt gccgatcagc cagaaaaaat tgaacaaaaa
    27541 attgaatacc tgatgaagca tgtgatcgat gggctgattg tcttcttatc cgaagaagag
    27601 tggtctggat ttgaaatgct cacaaaaatg agtattccag ttatcgcatt gaattgtcca
    27661 gaaggaccag caggtattga tacaatatta gtgaatgatc gagaaagtgt agcaaaagtg
    27721 atcgctcatc atggtgaaca aggacataaa aaaatcggat ttattgctgc cactcaaaca
    27781 gactacgtag caagagaacg tttagcagga gccaaagaag cggtccaaaa tgatccatcg
    27841 atccaattag aggtctttac aggagactat tctagaaaaa gcggctatta cggggcaaaa
    27901 gcattattag aaaaaggggc aactgctatt tttgtcagca attacaatat gtcgattggt
    27961 gccatcgaat gtttcaatca agaaggtgtc agaatcggga aggatattgc gttcagtcac
    28021 tatgattatt tagataaaaa cgatctgagt atccttccga aaatcacgat cacgccacca
    28081 acagataaaa tcggggaacg agcagcagaa cagctgttaa aacgaatcaa aaaagaagag
    28141 gaagaaaatg aaaaagtgat tgtgatggaa aatatcatac atggaatcaa ctaaaacgaa
    28201 ctaaaaaaga aaaagaggtg ctttgatgaa aaaatatcaa tttccagcaa atttttggtg
    28261 gggttctgca gcaagtggcc cacaaacaga aggaaggatt tcaggcgatg gaaaaggaga
    28321 gaacattttt gattattggt acaaaaaaga accgcaaaag ttctttgatc aagtaggacc
    28381 tgaaaaaacc tcgcaagttt atacgaagta tcaagaggat gtacagttga tgaaacagac
    28441 tgggcataat tcatttcgaa catctatcca atggagccgt ttgatacctg atgaatctgg
    28501 aacaatcaac gaagaagccg ttcgtttcta tcatcgttat tttgatgagt tgattgaaaa
    28561 cggtatcgaa ccctttgtga atctgtatca tttcgatatg ccgatgtatc tgcaagaaaa
    28621 aggcggatgg ttgaaccgag aaacggttga agcctatgaa aaatatgcca aaagttgttt
    28681 tgaattgttt ggttcgaaag taaaaaaatg gtttactcat aatgaaccaa tcgttccagt
    28741 cgaagcaggt tatttatatc gatggcatta tccagaagag tcagatatga aaaaagcgat
    28801 ccaagttggc tatcatgaag cgttggcttc agcgttagcc atcaaatctt accatgaaat
    28861 ggagcaaggt ggtgttattg gtatcatctt gaatctgaca ccaagctatc caagagacga
    28921 atcaaaccct gaagatgtta aagcagcgca aatagcagat gcgttcttca atcgttcatt
    28981 ccttgatcca gctgtaaaag gcgagtttcc aagtgaattg attgagatag taaaagaact
    29041 tgatctcatg cctgtgatcc atgcagagga tttgacggtt atcaaagaaa atacagtaga
    29101 tcttttaggt atcaattatt accagccacg ccgggtaaaa gcaaaagaaa caccaatcga
    29161 tactaaaaac ggtccaatgc ctgaggatta tttcgacaat tatgaaatgc ctggtcgtaa
    29221 aatgaatcct tatcgcggat gggagatcta tgaacaagga atctatgata ttttgatcaa
    29281 tgtacgagat cattatggaa atatcgactg ctttatttct gaaaacggta tgggtgtaga
    29341 aaacgaagca cgatttatca aagaagatgg catgatccac gatgattatc ggatcgaatt
    29401 tgtccaatct catctgaaat atgtccatca agccattcaa gaaggtgcca attgtttagg
    29461 ctatcatatg tggacatgta tggataactg gtcgtggacc aatgcatata agaatcgata
    29521 tggctttatt tcagtagact tagcacaaga tggaaaaaga acaatcaaaa aatcgggtca
    29581 ttggttcaaa gacgtatcag ataaaaacgg atttgaggct taagaaagaa tagttgagag
    29641 caaatgcgtg aaattctcca ttcttaccat tttgtgaaaa agaagttcaa ttatttacga
    29701 tctttttcaa cttttttgtc tttctatttc attttttgta tataatagaa tagtcaaaat
    29761 taaataggag tggaggaaag catgaggaaa aaaagaccgt ttcttctctt ttggttattc
    29821 ggttttttct tactgcttat tttatccgca gcattttcag gaaaaatgac agcagatctt
    29881 gctcagaaag aaacagaaaa aatcgtagca agcaaagaat cagtagataa tttcaaaaaa
    29941 gaattctcaa cttattggtg ggcggagtat aaaataactg ccaattacga aaaaggcgaa
    30001 ttggacattt atttgccaaa aggaaaagaa gaaaatcaat ttattaatat cgatgacgta
    30061 cttagtgcga tttctctcag acaatatcaa tttaaagaca agcatttgac cgttttgctt
    30121 ttgactcatg atggcgacaa agtagcccgt ttgaaaaatg gaaaagtgat caccgcttat
    30181 tctgaaaaag tagacaaaaa agtggtcgcc gatcgtttag cggaatggaa aaagacaaat
    30241 gaacaagtca agaaaacaac tgtctcttct caaaaaaacg agtcgacagg aacaagtcct
    30301 tcagattctg gcagtggaca aacgataacg ggaagtgaag aaaatcaagc ctcaattatt
    30361 acgaatgagt catcacaagg acaatatcaa tcctatggac aatgaaaaa
//
LOCUS       DACCPY010000026        29895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCPY010000026 DACCPY010000000
VERSION     DACCPY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.026"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..644
                     /locus_tag="IVO69_001019"
     CDS             <1..644
                     /locus_tag="IVO69_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7399812.1"
                     /translation="PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKY
                     SRGEILVSKWITLVIYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTY
                     TLGSYIGLWLILSLVLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKW
                     NPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(825..2249)
                     /locus_tag="IVO69_001020"
     CDS             complement(825..2249)
                     /locus_tag="IVO69_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399813.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            2460..2906
                     /gene="nrdI"
                     /locus_tag="IVO69_001021"
     CDS             2460..2906
                     /gene="nrdI"
                     /locus_tag="IVO69_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7399814.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(2971..4953)
                     /locus_tag="IVO69_001022"
     CDS             complement(2971..4953)
                     /locus_tag="IVO69_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7399815.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4967..5863)
                     /gene="murQ"
                     /locus_tag="IVO69_001023"
     CDS             complement(4967..5863)
                     /gene="murQ"
                     /locus_tag="IVO69_001023"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7399816.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(5866..6927)
                     /locus_tag="IVO69_001024"
     CDS             complement(5866..6927)
                     /locus_tag="IVO69_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7399817.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(7138..7665)
                     /locus_tag="IVO69_001025"
     CDS             complement(7138..7665)
                     /locus_tag="IVO69_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7399818.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(7732..9234)
                     /locus_tag="IVO69_001026"
     CDS             complement(7732..9234)
                     /locus_tag="IVO69_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7399819.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(9492..10613)
                     /locus_tag="IVO69_001027"
     CDS             complement(9492..10613)
                     /locus_tag="IVO69_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7399820.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            10739..11680
                     /locus_tag="IVO69_001028"
     CDS             10739..11680
                     /locus_tag="IVO69_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7399821.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(11830..12726)
                     /locus_tag="IVO69_001029"
     CDS             complement(11830..12726)
                     /locus_tag="IVO69_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7399822.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            12995..14335
                     /locus_tag="IVO69_001030"
     CDS             12995..14335
                     /locus_tag="IVO69_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399823.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            14553..14996
                     /locus_tag="IVO69_001031"
     CDS             14553..14996
                     /locus_tag="IVO69_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7399824.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(15500..16918)
                     /gene="nhaC"
                     /locus_tag="IVO69_001032"
     CDS             complement(15500..16918)
                     /gene="nhaC"
                     /locus_tag="IVO69_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7399825.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(17321..18544)
                     /locus_tag="IVO69_001033"
     CDS             complement(17321..18544)
                     /locus_tag="IVO69_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7399826.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(18575..19414)
                     /locus_tag="IVO69_001034"
     CDS             complement(18575..19414)
                     /locus_tag="IVO69_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7399827.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(19431..20111)
                     /locus_tag="IVO69_001035"
     CDS             complement(19431..20111)
                     /locus_tag="IVO69_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399828.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(20113..21186)
                     /locus_tag="IVO69_001036"
     CDS             complement(20113..21186)
                     /locus_tag="IVO69_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399829.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(21833..22504)
                     /locus_tag="IVO69_001037"
     CDS             complement(21833..22504)
                     /locus_tag="IVO69_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7399830.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(22591..25269)
                     /gene="mgtA"
                     /locus_tag="IVO69_001038"
     CDS             complement(22591..25269)
                     /gene="mgtA"
                     /locus_tag="IVO69_001038"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7399831.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            25928..26365
                     /gene="ndk"
                     /locus_tag="IVO69_001039"
     CDS             25928..26365
                     /gene="ndk"
                     /locus_tag="IVO69_001039"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7399832.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(26387..27253)
                     /locus_tag="IVO69_001040"
     CDS             complement(26387..27253)
                     /locus_tag="IVO69_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7399833.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(27413..29506)
                     /locus_tag="IVO69_001041"
     CDS             complement(27413..29506)
                     /locus_tag="IVO69_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7399834.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         8614 a         6306 c         5255 g         9720 t
ORIGIN      
        1 atccagttga aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga
       61 ttgcagctgc aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc
      121 tttatcgaaa gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt
      181 attctgttta tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt
      241 tcccttcgat ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt
      301 tcacttatac tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga
      361 ttgcttgctt tatcaatagt tccggcgcat ccatctcagc aggaatcgtc ttttattttg
      421 cttcgtcaat catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat
      481 ggaatccgat cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc
      541 aattgactca tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt
      601 tcttagcatt aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag
      661 aggctgtgaa agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat
      721 tctgaaaaag gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa
      781 taaacgacag gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc
      841 ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc
      901 ttatctaagt ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa
      961 taaatgcctg gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc
     1021 atagcccata cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc
     1081 cgctgataag aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct
     1141 gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa
     1201 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
     1261 agttttcccc atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc
     1321 ggtaagatcg tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc
     1381 acgactgctc cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc
     1441 ttgatcgttt gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg
     1501 taaataataa tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt
     1561 tttgctcgtg aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc
     1621 atgatgcaac tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata
     1681 atgtcgctag aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca
     1741 ataaataaaa ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca
     1801 ttttcgactc cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg
     1861 atcgatgcca gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc
     1921 aacaatgttg caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg
     1981 ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa
     2041 tctggacgtt ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct
     2101 acgagtaccc aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata
     2161 ccaaatacgc cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca
     2221 attccttttt tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta
     2281 tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat
     2341 ataattctac cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta
     2401 caccaccaaa aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca
     2461 tgaacatttt atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag
     2521 aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa
     2581 tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt
     2641 atttagacgg cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc
     2701 gtgaatatct ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa
     2761 ataaaaactt caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct
     2821 ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc
     2881 agattttagc agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt
     2941 tcttctttcg tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt
     3001 tgcttttgct ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac
     3061 caagacgatc atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc
     3121 tacaagcgta tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc
     3181 gcctaagtca actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt
     3241 gatcccaata gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata
     3301 gatatgtccg ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac
     3361 tggatcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt
     3421 cacactttca gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt
     3481 ttcagtcgct aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc
     3541 aacgaagcca ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc
     3601 agagatcaat gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc
     3661 gatcattccg atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg
     3721 tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg
     3781 taaagctcct ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc
     3841 caatgctgca cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg
     3901 accagtttca tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat
     3961 aaccatcggc aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag
     4021 tacccagttg atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa
     4081 gaaaatttca atcagaccga tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac
     4141 cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa
     4201 gatgacacct aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt
     4261 cagtggtgct tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag
     4321 agttggtgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct
     4381 aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc
     4441 aagtgttccg gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt
     4501 accgacaaat gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt
     4561 gattttagat ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt
     4621 tacacggtct tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt
     4681 ttctcctaat ttcacgcctg ccaaatcaac catttgctgt gctactttgt tcactttccc
     4741 aggtccgatg atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc
     4801 ttttaattca gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat
     4861 gcagtggaca atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc
     4921 cagttcttcc aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt
     4981 ttacaaatcc gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca
     5041 aaatcatgac gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg
     5101 tttcgtaatc agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt
     5161 catttgttgg tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac
     5221 tgatagtaga aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag
     5281 agcctgtcaa aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga
     5341 tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat
     5401 ctaatccacc gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat
     5461 tttttgtcaa attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc
     5521 cttcgacagc tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg
     5581 gttctacacc gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg
     5641 ttccggctcc catataaatc agacgtccac cttttttaaa ggactcaatc gtgttagcaa
     5701 tcactggttc gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt
     5761 tgttcattag ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt
     5821 ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt
     5881 tcttataaat tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt
     5941 gataacttta gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg
     6001 gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg
     6061 cgtagctgta tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg
     6121 tatgacatct cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa
     6181 ttcttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa
     6241 ttgctcctga acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact
     6301 tgctaataga tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg
     6361 caatccttga tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag
     6421 gagaccttga attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt
     6481 ttcgggacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc
     6541 tttcaattca tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg
     6601 atgtgaccag tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac
     6661 accaattgct ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc
     6721 tcctgaaata tcgaccatca attctagttt attctttttc gcttcgcttc ctagttcgat
     6781 caatcgcttt tgatatgccg aaacatcatc ttctggtata tgcatggaag tgaaaatacc
     6841 gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc
     6901 gttcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt
     6961 ctatgactag agtatatatc taaagaaaca aaatttcaat aattttatta aaataattaa
     7021 atttcatttc taaaggttga gtttatatta tcaaaagaat tgtcataacc taaaaaaggc
     7081 actgctaagc aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta
     7141 ctcataagaa accaattctg tattatctac catgaatgta aagaacccct cattaggcat
     7201 cggatgaaaa taagcattga tcactcttat caaggcatta tggcaaacta acagatagac
     7261 attttcttcg tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa
     7321 gactgactcc ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc
     7381 atctttgctt ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac
     7441 ggtcagcggc aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag
     7501 aggggaatgg atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt
     7561 ctgtgcctgc atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc
     7621 ttgtgcgttc caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc
     7681 gttggcaaag ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct
     7741 ctcaagagaa ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag
     7801 taacatggca ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc
     7861 caaataattc aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc
     7921 caattccact tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc
     7981 tctttccatc ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt
     8041 ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat
     8101 gctcagatgt tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag
     8161 ataatggata atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg
     8221 aggctcgatt gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc
     8281 ttctacgctt aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat
     8341 atattcgata tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac
     8401 gattgctttg gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac
     8461 tcgtctagat tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga
     8521 acgagaccat tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa
     8581 tttttcattt tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga
     8641 gaaattccct tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac
     8701 ttcatcgata aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa
     8761 ataagccgct acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa
     8821 cgggaaatga gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc
     8881 cgatagtgtg ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc
     8941 tctttcgatt tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg
     9001 ttctttatct tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc
     9061 ttgataaggt agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg
     9121 tccgataagt cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa
     9181 tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca
     9241 tcacctcgta aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt
     9301 ggatacacac aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca
     9361 ctgcttcctt gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt
     9421 ttcaatgcac ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat
     9481 gattttgtca actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt
     9541 tttgtttttt ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa
     9601 tgaatactta agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt
     9661 ccgcaaatag cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga
     9721 acttttccgt ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct
     9781 tgttcgatct ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc
     9841 ttcgctccca acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata
     9901 tcatctaaat ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta
     9961 tctaagaaag ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct
    10021 ccttgttgac ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa
    10081 acaagaacgt ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct
    10141 gtgtaccctt ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac
    10201 ccgcttcctc ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata
    10261 agttctccta aggcataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt
    10321 agatctaacc ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt
    10381 gtcaataaat agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc
    10441 catgttcccc tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc
    10501 ggcacattga tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt
    10561 tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc
    10621 cctcactttc ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat
    10681 tttcttccat ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat
    10741 gaaaatcgca atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca
    10801 aagcggaaat gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac
    10861 aaatgggttg attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc
    10921 accagaagaa atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc
    10981 caatcaatta gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt
    11041 tttgtgttta ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa
    11101 aaatatctta tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac
    11161 attgttaggt gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac
    11221 aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt
    11281 tcgttattcc atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat
    11341 tttggattgt aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac
    11401 aatcgtatct gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga
    11461 agtgtaccaa catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc
    11521 tatgtaccaa gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc
    11581 catttggcga aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca
    11641 attagtccat gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc
    11701 agctgaatca gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac
    11761 aactctcatg atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat
    11821 gtaggttttt tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt
    11881 ggaatcgtta gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc
    11941 tgataggatt ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc
    12001 tcgaagcaaa aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc
    12061 cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg
    12121 accctcccgc aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc
    12181 ataagtaaga tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt
    12241 aacatctctt tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt
    12301 cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga
    12361 ataaaatgaa ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata
    12421 aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt
    12481 gaagtgattc caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc
    12541 catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg
    12601 tctagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga
    12661 gcggatggaa ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt
    12721 gttcatcttt tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag
    12781 taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata
    12841 tgatgaaata aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat
    12901 tgtctgaaaa tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa
    12961 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
    13021 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
    13081 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
    13141 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
    13201 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
    13261 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
    13321 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
    13381 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
    13441 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
    13501 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
    13561 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
    13621 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
    13681 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
    13741 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
    13801 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
    13861 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
    13921 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
    13981 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
    14041 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
    14101 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
    14161 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
    14221 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
    14281 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
    14341 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
    14401 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
    14461 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
    14521 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
    14581 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
    14641 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
    14701 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
    14761 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
    14821 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
    14881 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
    14941 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
    15001 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
    15061 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
    15121 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
    15181 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
    15241 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
    15301 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
    15361 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
    15421 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
    15481 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
    15541 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
    15601 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
    15661 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
    15721 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
    15781 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
    15841 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
    15901 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
    15961 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
    16021 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
    16081 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
    16141 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
    16201 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
    16261 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
    16321 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
    16381 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
    16441 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
    16501 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
    16561 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
    16621 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
    16681 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
    16741 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
    16801 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
    16861 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
    16921 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
    16981 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
    17041 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
    17101 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
    17161 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
    17221 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
    17281 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
    17341 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
    17401 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
    17461 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
    17521 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
    17581 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
    17641 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
    17701 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
    17761 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
    17821 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
    17881 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
    17941 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
    18001 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
    18061 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
    18121 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
    18181 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
    18241 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
    18301 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
    18361 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
    18421 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
    18481 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
    18541 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
    18601 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
    18661 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
    18721 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
    18781 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
    18841 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
    18901 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
    18961 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
    19021 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
    19081 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
    19141 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
    19201 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
    19261 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
    19321 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
    19381 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
    19441 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
    19501 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
    19561 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
    19621 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
    19681 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
    19741 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
    19801 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
    19861 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
    19921 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
    19981 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
    20041 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
    20101 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
    20161 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
    20221 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
    20281 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
    20341 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
    20401 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
    20461 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
    20521 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
    20581 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
    20641 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
    20701 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
    20761 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
    20821 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
    20881 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
    20941 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
    21001 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
    21061 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
    21121 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
    21181 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
    21241 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
    21301 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
    21361 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
    21421 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
    21481 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
    21541 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
    21601 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
    21661 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
    21721 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
    21781 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
    21841 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
    21901 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
    21961 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
    22021 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
    22081 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
    22141 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
    22201 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
    22261 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
    22321 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
    22381 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
    22441 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
    22501 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
    22561 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
    22621 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
    22681 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag
    22741 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
    22801 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
    22861 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
    22921 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    22981 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    23041 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    23101 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    23161 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    23221 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    23281 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    23341 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    23401 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    23461 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    23521 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    23581 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    23641 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    23701 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    23761 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    23821 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    23881 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    23941 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    24001 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    24061 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    24121 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    24181 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    24241 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    24301 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    24361 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    24421 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    24481 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    24541 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    24601 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    24661 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    24721 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    24781 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    24841 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    24901 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    24961 atctccagga gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa
    25021 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    25081 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    25141 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    25201 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    25261 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    25321 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    25381 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    25441 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    25501 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    25561 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    25621 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    25681 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    25741 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    25801 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    25861 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    25921 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    25981 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    26041 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    26101 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    26161 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    26221 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    26281 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    26341 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    26401 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    26461 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    26521 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    26581 gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    26641 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    26701 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    26761 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    26821 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    26881 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    26941 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    27001 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    27061 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    27121 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    27181 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    27241 caccatcata cataatagtt ctccttttta atttaaattt ttccacattc aataaataga
    27301 tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac
    27361 caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat
    27421 tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat
    27481 taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat
    27541 tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg
    27601 gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct
    27661 tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga
    27721 tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa
    27781 agttcccatt tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa
    27841 aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga
    27901 tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca
    27961 atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa
    28021 gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca
    28081 caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg
    28141 attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca
    28201 aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa
    28261 gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata
    28321 aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat
    28381 caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta
    28441 cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg
    28501 cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg
    28561 tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga
    28621 aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt
    28681 cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga
    28741 tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct
    28801 ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag
    28861 tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa
    28921 tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta
    28981 gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga
    29041 cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca
    29101 cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg
    29161 acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga
    29221 attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca
    29281 aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata
    29341 aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta
    29401 atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca
    29461 aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc
    29521 tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt
    29581 atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac
    29641 cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac
    29701 tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa
    29761 ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa
    29821 acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc
    29881 tgttttttga aattg
//
LOCUS       DACCPY010000027        29358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCPY010000027 DACCPY010000000
VERSION     DACCPY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.027"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IVO69_001042"
     CDS             85..972
                     /locus_tag="IVO69_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7399835.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IVO69_001043"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IVO69_001043"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7399836.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IVO69_001044"
     CDS             2274..2720
                     /locus_tag="IVO69_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7399837.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IVO69_001045"
     CDS             2993..4345
                     /locus_tag="IVO69_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7399838.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IVO69_001046"
     CDS             4516..4803
                     /locus_tag="IVO69_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399839.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IVO69_001047"
     CDS             5029..5472
                     /locus_tag="IVO69_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7399840.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IVO69_001048"
     CDS             complement(5478..6662)
                     /locus_tag="IVO69_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7399841.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IVO69_001049"
     CDS             6930..7628
                     /locus_tag="IVO69_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7399842.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IVO69_001050"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IVO69_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7399843.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IVO69_001051"
     CDS             complement(8438..8971)
                     /locus_tag="IVO69_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399844.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IVO69_001052"
     CDS             complement(9268..11649)
                     /locus_tag="IVO69_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7399845.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IVO69_001053"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IVO69_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7399846.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IVO69_001054"
     CDS             12350..12889
                     /locus_tag="IVO69_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7399847.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IVO69_001055"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IVO69_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7399848.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IVO69_001056"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVO69_001056"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IVO69_001057"
     CDS             13996..15162
                     /locus_tag="IVO69_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7399849.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IVO69_001058"
     CDS             15208..16704
                     /locus_tag="IVO69_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7399850.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IVO69_001059"
     CDS             16785..17468
                     /locus_tag="IVO69_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7399851.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IVO69_001060"
     CDS             18113..19303
                     /locus_tag="IVO69_001060"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7399852.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IVO69_001061"
     CDS             19405..20358
                     /locus_tag="IVO69_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7399853.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IVO69_001062"
     CDS             20427..21776
                     /locus_tag="IVO69_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399854.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IVO69_001063"
     CDS             complement(21959..22282)
                     /locus_tag="IVO69_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399855.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IVO69_001064"
     CDS             22411..23574
                     /locus_tag="IVO69_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399856.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IVO69_001065"
     CDS             23644..24993
                     /locus_tag="IVO69_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7399857.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IVO69_001066"
     CDS             complement(25038..25469)
                     /locus_tag="IVO69_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7399858.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IVO69_001067"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IVO69_001067"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7399859.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IVO69_001068"
     CDS             28699..29295
                     /locus_tag="IVO69_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7399860.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
BASE COUNT         9822 a         5066 c         6139 g         8331 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaa
//
LOCUS       DACCPY010000028        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCPY010000028 DACCPY010000000
VERSION     DACCPY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.028"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IVO69_001069"
     CDS             complement(<1..1030)
                     /locus_tag="IVO69_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7399861.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IVO69_001070"
     CDS             complement(1112..1801)
                     /locus_tag="IVO69_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7399862.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVO69_001071"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVO69_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7399863.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IVO69_001072"
     CDS             complement(2328..2831)
                     /locus_tag="IVO69_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7399864.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVO69_001073"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVO69_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7399865.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVO69_001074"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVO69_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7399866.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVO69_001075"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVO69_001075"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7399867.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVO69_001076"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVO69_001076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7399868.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IVO69_001077"
     CDS             complement(6443..7462)
                     /locus_tag="IVO69_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7399869.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IVO69_001078"
     CDS             complement(7635..8522)
                     /locus_tag="IVO69_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7399870.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVO69_001079"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVO69_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7399871.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVO69_001080"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVO69_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7399872.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVO69_001081"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVO69_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7399873.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IVO69_001082"
     CDS             9753..10397
                     /locus_tag="IVO69_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7399874.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IVO69_001083"
     CDS             complement(10453..10956)
                     /locus_tag="IVO69_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7399875.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IVO69_001084"
     CDS             11553..12518
                     /locus_tag="IVO69_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7399876.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IVO69_001085"
     CDS             complement(12617..13789)
                     /locus_tag="IVO69_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399877.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IVO69_001086"
     CDS             complement(13776..14921)
                     /locus_tag="IVO69_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399878.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IVO69_001087"
     CDS             complement(14935..15867)
                     /locus_tag="IVO69_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399879.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IVO69_001088"
     CDS             15984..17063
                     /locus_tag="IVO69_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7399880.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IVO69_001089"
     CDS             17060..17689
                     /locus_tag="IVO69_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7399881.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IVO69_001090"
     CDS             complement(17799..18026)
                     /locus_tag="IVO69_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7399882.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IVO69_001091"
     CDS             complement(18129..20717)
                     /locus_tag="IVO69_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7399883.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IVO69_001092"
     CDS             complement(20737..21396)
                     /locus_tag="IVO69_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7399884.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVO69_001093"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVO69_001093"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7399885.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IVO69_001094"
     CDS             complement(22169..23035)
                     /locus_tag="IVO69_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7399886.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IVO69_001095"
     CDS             23144..23785
                     /locus_tag="IVO69_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7399887.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVO69_001096"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVO69_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7399888.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IVO69_001097"
     CDS             complement(25226..26125)
                     /locus_tag="IVO69_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7399889.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IVO69_001098"
     CDS             complement(26127..26924)
                     /locus_tag="IVO69_001098"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7399890.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IVO69_001099"
     CDS             complement(26902..27606)
                     /locus_tag="IVO69_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7399891.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IVO69_001100"
     CDS             27737..28312
                     /locus_tag="IVO69_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7399892.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IVO69_001101"
     CDS             28430..>29103
                     /locus_tag="IVO69_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7399893.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACCPY010000029        28171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCPY010000029 DACCPY010000000
VERSION     DACCPY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.029"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            611..2350
                     /gene="yfcC"
                     /locus_tag="IVO69_001102"
     CDS             611..2350
                     /gene="yfcC"
                     /locus_tag="IVO69_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7399894.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2507..3691
                     /gene="iadA"
                     /locus_tag="IVO69_001103"
     CDS             2507..3691
                     /gene="iadA"
                     /locus_tag="IVO69_001103"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7399895.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3802..4005
                     /locus_tag="IVO69_001104"
     CDS             3802..4005
                     /locus_tag="IVO69_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7399896.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(4106..4768)
                     /locus_tag="IVO69_001105"
     CDS             complement(4106..4768)
                     /locus_tag="IVO69_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399897.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4781..6283)
                     /locus_tag="IVO69_001106"
     CDS             complement(4781..6283)
                     /locus_tag="IVO69_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399898.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6831..8510
                     /locus_tag="IVO69_001107"
     CDS             6831..8510
                     /locus_tag="IVO69_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7399899.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8637..9116
                     /locus_tag="IVO69_001108"
     CDS             8637..9116
                     /locus_tag="IVO69_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7399900.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9335..10015
                     /locus_tag="IVO69_001109"
     CDS             9335..10015
                     /locus_tag="IVO69_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7399901.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            10023..11354
                     /locus_tag="IVO69_001110"
     CDS             10023..11354
                     /locus_tag="IVO69_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7399902.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11369..12331
                     /locus_tag="IVO69_001111"
     CDS             11369..12331
                     /locus_tag="IVO69_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7399903.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12442..12780
                     /locus_tag="IVO69_001112"
     CDS             12442..12780
                     /locus_tag="IVO69_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7399904.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12859..13566
                     /locus_tag="IVO69_001113"
     CDS             12859..13566
                     /locus_tag="IVO69_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7399905.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13669..15150)
                     /locus_tag="IVO69_001114"
     CDS             complement(13669..15150)
                     /locus_tag="IVO69_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7399906.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15459..15995
                     /locus_tag="IVO69_001115"
     CDS             15459..15995
                     /locus_tag="IVO69_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7399907.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            16073..17443
                     /locus_tag="IVO69_001116"
     CDS             16073..17443
                     /locus_tag="IVO69_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7399908.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17510..17845
                     /locus_tag="IVO69_001117"
     CDS             17510..17845
                     /locus_tag="IVO69_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399909.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17926..18783)
                     /locus_tag="IVO69_001118"
     CDS             complement(17926..18783)
                     /locus_tag="IVO69_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7399910.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18890..19405
                     /gene="tadA"
                     /locus_tag="IVO69_001119"
     CDS             18890..19405
                     /gene="tadA"
                     /locus_tag="IVO69_001119"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7399911.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19428..19512
                     /gene="ffs"
                     /locus_tag="IVO69_001120"
     ncRNA           19428..19512
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVO69_001120"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19722..21107
                     /locus_tag="IVO69_001121"
     CDS             19722..21107
                     /locus_tag="IVO69_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7399912.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            21144..21821
                     /locus_tag="IVO69_001122"
     CDS             21144..21821
                     /locus_tag="IVO69_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7399913.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21829..22593
                     /locus_tag="IVO69_001123"
     CDS             21829..22593
                     /locus_tag="IVO69_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7399914.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22595..23254
                     /locus_tag="IVO69_001124"
     CDS             22595..23254
                     /locus_tag="IVO69_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7399915.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(23284..23793)
                     /locus_tag="IVO69_001125"
     CDS             complement(23284..23793)
                     /locus_tag="IVO69_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7399916.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23905..24903)
                     /locus_tag="IVO69_001126"
     CDS             complement(23905..24903)
                     /locus_tag="IVO69_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7399917.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            25104..25688
                     /locus_tag="IVO69_001127"
     CDS             25104..25688
                     /locus_tag="IVO69_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7399918.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25765..26940)
                     /locus_tag="IVO69_001128"
     CDS             complement(25765..26940)
                     /locus_tag="IVO69_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7399919.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            27022..27402
                     /locus_tag="IVO69_001129"
     CDS             27022..27402
                     /locus_tag="IVO69_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7399920.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27476..27838
                     /locus_tag="IVO69_001130"
     CDS             27476..27838
                     /locus_tag="IVO69_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399921.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9200 a         4726 c         5847 g         8398 t
ORIGIN      
        1 aaagtttgtt atttttataa gttgtttcac agttatgaga aatgagttat aagaatatgt
       61 agtgtcagaa aaataaaaag tagtggaaaa agagacaaga aagaatccat atggattctt
      121 tcttgtctct taatacacgt tgatgaaaga gtcttcccca aaactcttta caaagtaatt
      181 atagtacatt tgttatcatt attgcaatga agtttacctg atataacaaa attgtccaac
      241 aggatacctt tttctaatag aaataaaata cacaaaaatt gaaggataga taatggatat
      301 ttataagtgt ttattatttt ttttgcaatg aaattcaatt tttcaatgat tgtaaaatga
      361 aaaaaacagg acgagatttt ctctaatcta aaaataaaaa aagaatttta aggaaaaaca
      421 gagaaataaa atacttaata taaatggaaa gagaaaaaaa taatcataca taggagaaaa
      481 aaaacaaaat agaaaaaagt gattttctaa taaattattc atgtggaaat gtcattttta
      541 ctgtataatt catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga
      601 agaaataaat ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt
      661 atcgacaccc catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac
      721 ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg
      781 cgaaacaagt actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga
      841 taagtcgtat gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa
      901 actggatgtt ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac
      961 gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga
     1021 aaatatctat gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga
     1081 ttttggcggc tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta
     1141 tggatctgct gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat
     1201 tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact
     1261 ggagcgttta gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg
     1321 gatggcagaa gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg
     1381 atatgactcg atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac
     1441 ctcttggatg agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt
     1501 gtctggtgcg acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta
     1561 tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc
     1621 agatgattat ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct
     1681 tgggcataaa ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt
     1741 gactcaaaaa ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt
     1801 tgctggaatc attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc
     1861 ttctttccaa tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa
     1921 agggattctt cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat
     1981 tttgcatggg atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat
     2041 gtacatcttc caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt
     2101 gactatgcca atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt
     2161 tcttgcttat cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt
     2221 gattggtgtg ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa
     2281 gatgcaagga ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg
     2341 tctgcagtag tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc
     2401 caagaaccgt cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt
     2461 tttaagatcc tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg
     2521 acaaattgaa gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg
     2581 gaagattttg gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga
     2641 actttcaggt gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt
     2701 aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt
     2761 gactacagct ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga
     2821 tatgactgcg ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt
     2881 atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt
     2941 tttgacaatt gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc
     3001 tcagccaagt tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt
     3061 atccggaaaa gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct
     3121 attgacacga atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc
     3181 aaatcgcaca ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat
     3241 tgacttcact gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt
     3301 cagcaaagca ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac
     3361 ctcagatgga caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg
     3421 agtaggaagt gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat
     3481 ccctttggaa atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga
     3541 taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac
     3601 gcttgatatt gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa
     3661 agtatggggg actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa
     3721 acgagcgttc tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac
     3781 aaactaagag gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat
     3841 aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa
     3901 acagaaggga aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc
     3961 gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact
     4021 aattgaatca agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt
     4081 ttttatttga tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta
     4141 gttcaaagac ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac
     4201 agcgattttg ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt
     4261 catctagatt tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac
     4321 gaacgatagc gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta
     4381 atatttcatc aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt
     4441 gcttggtctt agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa
     4501 gcaagttgta agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga
     4561 ttttgttcag tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag
     4621 aaataagcga aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt
     4681 ttccagcttc gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc
     4741 aaaatcccaa ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc
     4801 ttgggtttaa tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa
     4861 atgctactcc ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga
     4921 cttgatcaga aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc
     4981 cttgttcttg tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg
     5041 tggtttctgt ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc
     5101 taagcgaagc aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga
     5161 gaatgatttt acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct
     5221 cccgaatggt aatcataatg atcagagtaa gtatgataat accggctaca gccactagta
     5281 aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca
     5341 tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga
     5401 gaaactcatc cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat
     5461 cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg
     5521 gattcatgaa gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc
     5581 ctttgatcgt catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat
     5641 ctccgactgc caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc
     5701 ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat
     5761 tactattttg tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg
     5821 gattcccgcc cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt
     5881 cacttgaatc agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac
     5941 tttcaaagga ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg
     6001 aaactggtgt aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg
     6061 aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg
     6121 ctccaacttc tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc
     6181 cagctagcac aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc
     6241 attttacttt tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta
     6301 gtagatgcat ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg
     6361 cacaccatca acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg
     6421 tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa
     6481 aagaaatcct agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga
     6541 tactagcgat ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag
     6601 tattctggct atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa
     6661 aatgcccatc caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata
     6721 agttgagaaa tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag
     6781 aagaaaatcg acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca
     6841 ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag
     6901 tgggcgagga tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg
     6961 ggatcgacgt cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg
     7021 gtgtcttttt aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa
     7081 aaatttctgt accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca
     7141 gtcgtaatga agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa
     7201 tagactttgg tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag
     7261 tggggatcaa tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg
     7321 accaaacggc tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag
     7381 aaggagtctt agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt
     7441 ctgaagccca aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca
     7501 tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa
     7561 aagcacaacg aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga
     7621 tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga
     7681 aaaactatga tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca
     7741 agtctctgca aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt
     7801 tggtttatat cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag
     7861 atatcattta tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg
     7921 gccatgccaa tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg
     7981 tgccagttca aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag
     8041 ggatgccata caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca
     8101 agcgtatgag tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca
     8161 taggtgatat cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt
     8221 tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta
     8281 cttcacgggg attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata
     8341 tgattgaaac aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa
     8401 agcaagacat ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag
     8461 tgatcttgcc agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag
     8521 taagatgaac ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt
     8581 tttgaataca gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga
     8641 tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg
     8701 attttagtca tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc
     8761 cagctttgat atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat
     8821 tgattccttt gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta
     8881 aagttggttt tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa
     8941 acacccctat tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt
     9001 taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa
     9061 aagtagccga tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga
     9121 cgagaagaga agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact
     9181 tggtgcatcc tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg
     9241 aaaatatatt tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat
     9301 aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc
     9361 ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg
     9421 aaatccattg acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca
     9481 tggtcatagt gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat
     9541 tatcagtatt gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat
     9601 gaatatcggt ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca
     9661 tagttcaagc tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat
     9721 tattcaagca gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct
     9781 aggattgctt gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta
     9841 tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt
     9901 tccaatccat tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt
     9961 actggaagat agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga
    10021 aaatggcgac aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc
    10081 gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg
    10141 caatcaaaga tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc
    10201 gtatcgaacg caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga
    10261 tcattgaagg ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg
    10321 tcatcggcgc gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga
    10381 tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga
    10441 tcacaggagg attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc
    10501 cgatcttacg cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa
    10561 gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa
    10621 agactcatta tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa
    10681 cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg
    10741 caaaagatgt tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac
    10801 caatcagtgt aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg
    10861 ggctagaagt catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc
    10921 aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt
    10981 cggatcagat atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg
    11041 aaaaccctaa gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt
    11101 cttttggcgt acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc
    11161 aaaaacgaaa tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg
    11221 aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg
    11281 atatcgaagt gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa
    11341 tggaacgttc ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat
    11401 aaaaaaatac gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg
    11461 atcacaagta ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca
    11521 agtaggtgaa acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt
    11581 cttctaccaa ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga
    11641 tcaaagatat cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc
    11701 ttatggagat cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga
    11761 atttgctttg atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta
    11821 tgcaggaatc gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt
    11881 agagattgct tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat
    11941 tgaacaaatg ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt
    12001 ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat
    12061 cgatgattca gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc
    12121 ttgggggatt tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg
    12181 gccgatcatc aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg
    12241 tgcgaatgca aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg
    12301 ccaatcgtat aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag
    12361 taaaaatcac aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa
    12421 agattttaaa ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac
    12481 acatatgaag atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa
    12541 gaacaagaaa ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg
    12601 ttagagggcg atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc
    12661 attaaacaag gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat
    12721 tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa
    12781 agaatattca aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg
    12841 aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt
    12901 cttttggtca ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact
    12961 ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa
    13021 tatatattac aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc
    13081 acagataact acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct
    13141 acttcagttg ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct
    13201 tcgggttatg tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg
    13261 atgatttcgt tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc
    13321 aatatcggga ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc
    13381 gaatttaacg cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa
    13441 gaagaagtac cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca
    13501 gcgctttcga cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga
    13561 gattaaaata aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc
    13621 tgattgaata ccatttattt ggaataagag gatgtgacaa gaattttgtc acatcctctt
    13681 tgttttgttc tcataacctt ttttcatata cagttcatga accgctttta ttaacttgcc
    13741 aaataacact gtattgtgcg ttgtatccag atagaaagtc tccgtttcat ctgaaatttt
    13801 ctcatgctga tctgtaatga tcaaatcagc atgttctgaa tatgcggtaa ttttaacagc
    13861 atctttgcga aataactgct gcaaatggct ttgaagatga taatttcctg taaactgttt
    13921 ggttatctgc aaatggatga gtaactgctc tttcgtttct gtttgtagta agtgttggat
    13981 caattgacta aggtattgac tgatttggtt tctttcccca ttttttgaaa aaattgtatc
    14041 gattttttct cttaatttct tttcctgtat aaaagaacta gaatgattgg acgtggcttc
    14101 tggaaagcaa agtgtagaaa aactactgct ggattcaccc aacagttcga aaaaaacaac
    14161 tgccaacaca agaaaataaa aataaattgg tattttattt tctaaatggt tgagaggaaa
    14221 ctgttgagtt aagtaagtca tcaattgacc gacttgtcga caaataaggt ttttgcttgt
    14281 ggaaaataaa aaaccaatct cctgtttttg tcgaaaagag atttgatttg gaaaaaaaca
    14341 atagaaaaaa taattaaagt aggctctctc tgtttctgtc tcttcacaag ggcgactcac
    14401 tgaaaaagaa gtaaagtatt ctgaataatc atgtgtatgc tgcaataagt ccatcaattc
    14461 agtgatttca gaagaagttt ttggaaaata tgcctgttgt ttgtttcgta atatgaataa
    14521 agttaacagc aagtagatat ttttttcttg ttttgaaatt ttttctattt gctcatctgt
    14581 actgttcatt tttagtgcat ttaattcaag tgaggaaaat ggccaatcgt tacctccaaa
    14641 tgtatctgaa agaaaaaagt aaagatagag acggatagat aactcatttc cttcaagagt
    14701 acagcaattt tttacagata ggatccgtaa atggaatttt tttaaggaat gattgagctg
    14761 ttttgtcagt ttttcgagat aggagggcga tatataaagt tgttcaagta aatatttttt
    14821 tggtacagca gtttgtggat aattgattag taacatcaaa tagttaaaac gattggattc
    14881 ttttaggtag cataatttta acttagaaaa aacagctaac tttttagttt gatcggtttc
    14941 tttggtttgg atctctcctt tatgattaga ataaataaat tcatcaatca ataaaaattg
    15001 cttgagtgat acatttattt ttcggatcag ttctttgatt gtccgttttg gaatatgtaa
    15061 gtcattggat aattcttgta aagaacaatt atttttttga atgaccattt gtagaacaag
    15121 taactctttt tttcttgctt caattagcat aaatacctcc tttttattct gtttttgtat
    15181 atagttagac aaaaactgtg agtggaaagt catttcagtc tgtgttattc tcacagagtg
    15241 tcgccttctt ttttatataa cgttatttaa tttttttgtt accttgttta ttctattgtt
    15301 tatttcctag ggtattttgt caagatattc ttgtttttaa ggcggaaaaa ttgattttta
    15361 atggatataa ctaaatatgt tttgataatt atcaataata tttttagaat tatttatgtt
    15421 atttatttat gggtagatga aaagagacgg aggtaagaat gatggggaga gtaacaaatt
    15481 atagtaaaga atatttgctg ttcaaaagta tggtatacgt ggaagaatat ggaatgaagt
    15541 caattactgc aagagagtta gcagagttct gcggttgttc tacatacccg atttatacac
    15601 atttcaaatc aatcagcggg ttaaaagaga agatattaga agaaatcact gtttattttg
    15661 aaaactactt agctgaatgc agttccgatg atgttttaag cattgtctac ttattgaaag
    15721 attttttttc tatccatgaa agtattcgtg aaatcattgc aaaatctgat ttagacatgg
    15781 attcgctatt taagcggcca tttttctcat atgtcaaagg tcacacgaag atcaaagaag
    15841 aaaagtcgct tgaattggta tggcttaatg cattgggaag tttgcgctca gatgattctt
    15901 ctcaagattt tcttgaaatg atgaacgctg tttgggataa aaacgaggtg acggatgaat
    15961 acttggaaaa tatcttaaag gaacagtcac cttaggcagc aacatgaaaa agtagatcag
    16021 ccacctttat ttttcgtaaa aaagattgta aaatgtctaa cgaaacattg gaatgaagga
    16081 ggaagcgaca acgttaccac agcaatttat caaaaaatat cgtcttcttt taggcgaaga
    16141 agcatccgat tttttttctg cgcttgaaca aggaagcgtg aaaaaaggat ttcggtggaa
    16201 tccactaaaa ccggcaggat tggacatggt ccaaacttac catagcgaag aattacaacc
    16261 cgctccctac agcaatgagg gatttcttgg cactgtcaat ggaaagtctt tcctccatca
    16321 agctgggtat gaatatagcc aagaaccaag tgcaatgatc gttggaacag ctgctgcagc
    16381 aaaacccggt gaaaaagttt tggatttgtg tgcggctccc ggtggtaaat caacccaatt
    16441 agctgctcaa atgaagggga aagggttatt agtgacgaat gagatctttc cgaaacgtgc
    16501 gaagatttta tcagaaaata tagaacgatg gggagtttcg aacgcaatag tgacaaatca
    16561 tgcacccgca gaacttgtcc ctcatttttc gggctttttt gatcggatcg tggttgatgc
    16621 tccttgttca ggagaaggaa tgttccgaaa agatccaaat gcaataaaag agtggacaga
    16681 agaatctccg ctatactgtc aaaaacgtca gcaagagatt ttgtcttcag caataaaaat
    16741 gctaaaaaat aaaggacaac tgatttattc gacttgtacg tttgcaccag aagaaaatga
    16801 agagatcatt agctggcttg tagaaaatta tccagtaacg attgaggaga ttcctttgac
    16861 tcaatccgtt agttcaggac gttctgaatg gggaagcgta gctggtttgg aaaaaacaat
    16921 acgtatatgg cctcataaag atcagggtga aggacatttt gttgcaaagc tgacttttca
    16981 tggacaaaat cagatgcata aagaaaagaa gacacgaaaa aaaagtaaag tgcaaatgac
    17041 aaaagagcag gaaaaactct ggacggagtt ttcaaatgat tttcattatg aagctactgg
    17101 aagactttta gtgttcaacg accatctttg ggaagttcct gagttagccc cttctctcga
    17161 tggtttaaaa gtcgtacgta caggtttgca tttaggagat ttcaaaaaga atcgatttga
    17221 acctagctat gcattagcgc ttgctacaaa aaagatagag aatatacctt gcttgccgat
    17281 cactcaaaag gaatggcaat cttacacagc aggagaaaca tttcaacgtg atgggaatca
    17341 aggatgggtt ttgctagtat tagataagat acctgtcgga tttgggaaac aagtaaaagg
    17401 aacagtaaag aactttttcc ctaaaggatt gagatttcac tgagtggatt agttcgttta
    17461 tttttttgtc tatgctatga tggagataga taaaaaggag ctgatactta tgaaaacatc
    17521 aacaaaagta acaatcggat taagcgtagc agccgctgcc agtgtagcga cagcagtcgt
    17581 tgtatcggga aaagtaatgg aaaagatcca tcatatgact actcggacga aagtaaaaaa
    17641 attcgtctat gacaaatttg atggaaatga aaaattacta gaaatcgtag atggtctttc
    17701 agatagtgac ttggattctt tgatgggaat gttagcaaaa atcaaatcag gaaaaaagaa
    17761 aatctctgtt tatggtgatt caatcaaaga ttcaaccgaa gatgtaaaag accggctaat
    17821 gcattttgtt gaaaaaatga tgtaagagaa actagctttt gaacactatt cattcgaaat
    17881 aaggggttgt gacaaaatta ttgtcacaac ccctttaatc tatctttaat tatgttcatg
    17941 aagcaataaa agatagcaaa acagtaaatc gttggctttt tttagattga aaccagactg
    18001 ttcttgaaat ttttcgattc gatattttag agtattacga tgaagaaaca gtgtttttgc
    18061 agcagagctg acattgccta attcattcca taattcttga atgatcactt ccatttcatt
    18121 ttgtttgatc agttctatgt aacttttgat caacttacta tcttttacag tatcttttgt
    18181 atagtaatgt aaagccacat cttgcataga aaaagtacgg ctgtgagtat aaagattgtt
    18241 ttgctctgtt aaaaaaattt ttctttcttc agcaaacaaa gggacgaggt cggtgccagg
    18301 tgaatggaaa gaaccaacga atacagacgt tgtcgtatca aaatcagcat ctaaagatag
    18361 aaaaataccg ttgatttcag tagcatcaag ataatcacgg tttatttgtt cgactaagag
    18421 cgcatctgtg tctgtataaa aaaagaaatc agcagaatag cgaaataatt ccaaaatagt
    18481 atcttgccat tcttttttta agaattctcc ttttgattcg acgtgaagct ggatcaaacg
    18541 aaagcttttt tcttgaacac aagaagcatc tttaaaaaga tagtgatacc atgggtgttt
    18601 tttttgatcg ttgattacag ggaataacgc tttcaaaagg ctggtttctt gttgagataa
    18661 tgaatcttgt ttgatccaga taaaagcatc atcaattgca aaagaaagga tatccgcttc
    18721 tgatgctgga gagttttttt tctgtgcttg tgggtaaaga gcaagtaatt tatccatttt
    18781 catcttgttc acctcttgta tttattttac catagaaaaa aaagttagtt acagcctttt
    18841 tatcatatgc taaagtaaaa caagaaaaaa caaaggcagg gaagggtaat tggtaaatac
    18901 aaatcttgat gcagtagaaa aagaaaaatg gatgcgcctt gcattggcag aagcaaagaa
    18961 agcagaaatg cttcatgaag ttccgattgg agcagtggtt gtattggatg gaaaagtgat
    19021 tggaagagga tataacctta gagaaaccac gcaagatgct actacacatg cagaaatgct
    19081 tgcaatcaag gaagcctgtg aaaaagtggg aagctggcgc ttggaagatg ctgcactttt
    19141 tgttacctta gagccttgtc ctatgtgcag cggcgcgatg attttgtctc gtgtagcaga
    19201 agtttatttc ggcgcgtatg atccaaaagg cggaacagcc ggtaccttga tgaatctttt
    19261 ggaggacgag cggtttaacc accaagcata tgtagaaggt ggcattttag aagacgaatg
    19321 tgggatgatt ttgactgact ttttccgaaa gctccgccag cgaaaaaaga aattaaaaaa
    19381 agactggtca acgaatgaaa aataaggtat actattcttt gccgaaaggc taggacaatg
    19441 gtgggcttgt gaagcgtgtc aggtctggaa ggaagcagca ctaagcaggt tccgccatgt
    19501 gtctgattat aatgaaacga caacagactt tcgggtctgt tttttttatt tttatagtac
    19561 tgtaaaaatg aatgttcctg attttttagc cagactgatt tatcgaatat tgtacttata
    19621 caatttgctt caaggcagga aaaactctga aatagagctt gtttcaatgg aagaaaaagc
    19681 tgcctatggt atgataaaga taaagagagg cgagagggta catggaaaaa atagtgattt
    19741 tacatacgaa tgatctgcat tctcatttag aaaattggcc aaggatcaga aggtttttgg
    19801 atcaacgtaa acgagaaaac gaaaagaaag aaaaaacgac aacgattgca gtagatctag
    19861 gtgattttgt ggatcgttgg catccattgt cagaagcaac gaatggacag gaaaatatct
    19921 tgctgatgaa tgaagttggt tatgatgcag cgacgatagg aaacaatgaa ggcgtaggaa
    19981 attccaaaga agagttgaat catttatacg atcatgctga gtttgatatc atcctggata
    20041 acttattcga taaaaatacg ctccagccac cggcatggac gaaaccatat aaaatcatta
    20101 caacgaaaca ccagacgaaa atcggactaa ttgcatttac tgctccattt ccgcttactt
    20161 ataatccgaa tggctgggac atacggaatc cttatgatat tttgccagaa ttagtcgaag
    20221 atttacgttc acaagtggat gttttagttt tgatgagtca tctcgggatt caggatgatt
    20281 tccaaattgc aaaagaaata ccagcaattg atgttatttt aggatcgcac acccatcatt
    20341 tattcagaga aggtcacgta atcaatgatg tccaaatagc agccgctgga aagtacgggc
    20401 agtatgtcgg tgaagtccat ttaaccatcg atgaagagaa aaaaatcctt aagcattcag
    20461 ccaaagccat ccctaccgaa acaatgacag cttttacaga agatgaacaa gaaataaata
    20521 gttatctaga acgtgggcat gaattattaa aagaaaaaga agtagcagat ttgccatttt
    20581 ctctgagtct agatatcttc aatgagcatt cgtttatcca agtagccttt gaggcggtga
    20641 aagaacgagg aaagacggaa gcagcaatat taaacagtgg tctgttttta actgaaattc
    20701 caaaagggcg ggtcaatcag gatcagcttc acactgcgtt gccgcatccg atgcacttgt
    20761 taaatgtcac attgggtgga aatgatctga tccgcttagt tttagaaata gaaaaaaatc
    20821 gtaacttttt acgtaactat cctatgaagg gaatgggatt tcgcggtaag atttttgggc
    20881 agatggtcta taatggaatc acatatgatt ctattaacca tcaagtcttt tggaagaaca
    20941 agccaatcga tctgaataaa aattatactt ttactaccgt agatcatttg atgtttgttc
    21001 cattttttcc aacgattgag attgctggaa aaaatgaatt tttgttccct gaatttatcc
    21061 gaagtgtggt aggagattac ttgaaagctc actacccaat caaataaaaa caaggtataa
    21121 taaaaaagat aggtggtgaa cgcatgtctg caaaacaaaa tgaacagcag aacgttgagc
    21181 ataaaacaga aattgctaag aaaaaatcat cagctgaaca ggaaacaaca gtcaccgaag
    21241 aactgacagc tgctttggaa gaaatcgttg aggagcctaa agcagaaaaa gtcaaaggcg
    21301 agctagtgac actgttagat gacagtaatt ttttgatagg tgaaagacat tatcggcttg
    21361 tgtccgatta tcgtgaagga tttaatgcag aaaagcttgg agaacgttac agtgatgtac
    21421 tagcccgata tgattatatc gtaggagatt ggggttatga acaactacgc ctaaaaggat
    21481 ttttccaagc ggataatcga cgggcgcatc cagatcagcg aatcgattcc ttggaagact
    21541 atctttacga atattgcaat tttggttgtg cgtattttgt catcgagaga atcggcggta
    21601 aaaaagaaaa gacaactcag cgccgaaaaa agaaaaaaaa ccataatcgt gcgtatatcg
    21661 atgagaaaaa aggaccagtc gcatctaatc gcaaaaaacc ggttattaaa aatcgaaaaa
    21721 cagagtcagc aaaaacctct gaaaaagtaa aaaaacaaga acaacctaaa caagtagaaa
    21781 aaacaaaaac agcatttacg atcagaaaaa gggaagagta gtggatgaat ggactataaa
    21841 ggatatttaa ttgatttaga cggaacgatt tatcgtggga cacaacccat accggccggt
    21901 aaacgatttg tggaagaatt gcaaaaaaga aaattacctt tcttgtttgt taccaataac
    21961 acgactaagt caccagaaac agtagctaat cgtctagcag atgagtttga cattcatgta
    22021 gctccagaga cagtctacac agctactttg gcaacaatcg attttatgaa agcagatgga
    22081 aaagggaaaa aagtctatgt tatcggagaa gcaggattga ttgacttgat tttagctgcc
    22141 ggatttacat gggaagaaga ggctcctgac tatgtagtgg taggcttaga taattatctg
    22201 acctatgaaa aagtagttaa agcaacactt gctattcaaa aaggtgctac tttcattgga
    22261 acgaacccag ataagaatat acctactgaa cgcggccttc ttcctggagc agggtcagtc
    22321 atctcttttg tagagacagc aacccaaacg ccgccgatct acatcggaaa accagaagcc
    22381 atcattatgg ataaagccgt tgaagtatta gggctgcaaa aagaagaagt aatcatggtt
    22441 ggagataact atgaaacaga tatccaagct gggatacgca ataatataga tactttgctc
    22501 gttttatcag gttttacaaa agaagaagat gtacctgggt tgcctatccc agctacctat
    22561 gtgaaacaat cactggatga atggagcttc taatatgaaa aattcgagat ggcagtggat
    22621 ggaatatgct ggattgttca gcctatttct gacactgatc agtctggcag ttggtgtgac
    22681 gatcaacttt cgaccactat acgtatttga tatcggacat ttgcaaattt tggattatac
    22741 ttctttagat caagagacgt tactgaaaaa ttttgatcac ctaatgaatt atctcaataa
    22801 tcccttcaaa accatcctca gcttgccaga tttcccagtt tctgctagcg gagcccatca
    22861 cttttatgaa gtgaaaattt tgtttttagt ggattatgct gtgtttttca tcactttgat
    22921 cccgagtatt ttgtttatca aatatttgca aaagaatgat cggctttggc ggttgattcg
    22981 tccgtttcaa atcggcatgt tactgccagt tgttttcggt tttttcatga tgataggatt
    23041 tgatcgcttt tttatccttt ttcatgaaac gtttttcaac aacgatgact ggttattcga
    23101 tcccgttact gatccaatca tcaatgtact tccagaagaa tttttcatgc atagctttat
    23161 cttatttttt gtattattag aattattttt tgctgttttt cttttcttag gaaaaaatag
    23221 cttgaaacaa acaaaaaaga aagaactcgt ctaaatagac gttttctttc tttttttgtt
    23281 gaattattga gtaaggcgac ttcttttttc tggttctttg tgtgctaatc gactggcagc
    23341 agcagcggcg atcgctccga cgatatcatc taagaaggta tggacatgaa cgccatcgtg
    23401 gctgtttagt tcttttaaaa taccaggttt caccttgtcg atataaccat aattcgtaaa
    23461 gccgatcgta ccgtaaacat tcacgattga caaggctagg atctcatcga taccgtaaag
    23521 gccttcgtca tcttcgataa tatgttgcaa cggactcatc aattttttct tttctgctaa
    23581 tatatctaat tggattccag tgataatggc gttatgtact tcacgctttg ttagcacgct
    23641 gttgacactt tcagtacaat ccgccaaagt caaatcagaa atatagtctt tttgtaaaaa
    23701 catgacaagt tgtgcgatat cttctacagt tacgccacgt tccttcaata gttctcttgc
    23761 cttttcttct aaagtctcag ttttgacgac catattattg ctcccccttt attttttctt
    23821 tctctatttc atgttaacgg aacatgaaaa aaatgcaaga aactgctttc gggtttaact
    23881 tgaaatgcaa agagatataa aaaatcaact aggacgaaga tttttatcgt ataagcttgt
    23941 gctgtgacca ggctgagttc gttcctttgg attaatatag tgtaaagcgt tgttcactgc
    24001 agtaggtgct tcaccgagcc cagtagcaat cagtttcact tttccttcat aggtacagat
    24061 gtcaccagct gcataaacgc ctggaataga agtagacata tctgatttta cagtaatggc
    24121 atttctggta ctatcaagtc cccaagaaga aagatgttct aaattagaag agaatccata
    24181 attgacgatc agagaatcaa tcatcaaatc aatcgtctcg tcacttttca ctttttttaa
    24241 ccggatatct gtcagttccc cattattgcc tgataatcca tcgatgagat agggagtaag
    24301 caagttgaca gatgaagatt tcaagcgtga tacactatgt tcgtgcgcac gaaattccgg
    24361 gcgacgatga atcaagtaaa cttcgcttgc aataggctct aacatcaacg tccaatcgat
    24421 agcagaatca ccacctccag caatagcgac tttttttcca gcatacttca tcaaatcatt
    24481 cacgaaataa tccaatccgt ggttttcaaa tgattcagca ttatctaaat tcaattttct
    24541 tggctggaat gaaccatttc ctaaagctaa aatgactgct ttggaataat ggatgccttt
    24601 atttgtagta atctcgataa cttcgtcttc acgagtaagc gagagaactt cttcttttaa
    24661 atggaatgta tggttaaaag tagttaattg tttttctaat tgatcgatta attcacttgc
    24721 ttttattgct ggatacccag gaatatcgta aatatatttt tcgggataca aagttgctaa
    24781 ttgaccacca agttgaggta gactgtcaat gatttttgtt ttagcttggc gcattcctgc
    24841 gtaaaaagcg gcaaacatcc ctacaggacc tgcaccgatg attgtaatgt catagatttc
    24901 cataaaaatt cctccctttt cacactatag cagaatttaa ttgagaatgc ttctcaaaaa
    24961 tgtttgaatt cgaaatcgaa acggtaagtt atagaatgta tctagtgatg aaaaataaaa
    25021 gaaaacaaat ctagtatttt tacgagaggt taggtattat gttattatta ttatgaagta
    25081 tgcaaatatt aggaggaaac aatatggcat tcccacaact agatttaaaa agtgaaaaag
    25141 gaccaaaagc tgttatcaaa acaaaccgtg gtgacattac tgttcaatta ttccctgaac
    25201 tagcaccgaa gacggttcaa aatttcattg aactttccaa aaaaggttat tatgatggcg
    25261 tgatttttca ccgcgttatt cctgatttca tgatccaagg cggcgatcca actggaacag
    25321 gaatgggtgg cgaaagtatt tatggcgaaa gttttgaaga tgaattcagt cgtgaactgt
    25381 tcaaccttcg cggagcttta tctatggcaa attcaggtcc aaacacaaac ggaagccaat
    25441 tctttattgt aaataacaca aatgtgccag ctaatatgct tggacaacta gaaggtgctg
    25501 gattcccatc agaaatcatt gaagcgtata aaggtggcgg aactccttgg ctagatttcc
    25561 gacatacagt ctttggacat gtactagaag ggatggacac agtagatgaa atcgcaaatg
    25621 tacaacgtgg tccacaagac cgacctgtac atgatgtagt gattgaaaca attgaaattt
    25681 cagaatagtt tggtatggta cggagtaatt atgaaaaagg acgaaaccta gtaaggtctc
    25741 gtccttttat tttgaataaa tgatttaatc gattgtctct aaagcatact tgattcgatt
    25801 aagtccttcc agtaaagttt cttctggaca agcaatattc aagcgcatat gcccatgacc
    25861 ggaaggacca aacgtgatgc caggatttaa tacgactttt cctttccgaa caagagtgtt
    25921 ctccaattct tggtcgtctg aaatataggc ggtaaaatct aaccacatta agtaagttcc
    25981 ttcagggttc attattctca ctttcggaag atactgttca aaaaactgat taactgtttg
    26041 aacatttttt tctagataag gaatcagttg gcttaaccat tctccacccg tctcataagc
    26101 cgcttgggtt ccaatcagtc caaaagtgtt gatttcgtgc tgttggttca tcactaaatg
    26161 tttttcaaaa tcagctttca tctctggatt tttgataaat accattgaat ttttgattgc
    26221 agctagattg aaagtcttgg ttgcggaagt aaaagcaact agtagatcat ctaaattagg
    26281 atcaatcgtt agcatggaag taaatgtatg atcgaataaa gttaaatctt gatggatctc
    26341 gtcactaaac agaatcactt gatgttttaa acacaattcg cttaactgtt tcaattcttc
    26401 tttgctccaa acacgccctc caggattgtg aggattgcat aaaatcattg cttttacatt
    26461 gttttcttcg atttttttct ccatgtcaga caagtccatg atgaaatgat tatttttttc
    26521 tagcaaagag cttcgaacga tgtgacgttg atttgtttct ataatagagg aaaatggagg
    26581 ataaactggg tcatgaatca aaatagaatc gcctggtttt gtaaaagttt gtactgctgc
    26641 agctaaactg gttaacacac cacttgtaaa aacgatattt tctttcgtca actccacagc
    26701 atggtgttgg cgttcccaat gaatgatagc ctgatatagt ttatcagaca gagtggcata
    26761 accaaagatt ccatgtttga tgtattcact aaacgcttta gctaccccac cgggagacaa
    26821 gaagtccata tctgctaccc acaaaggaag taagtcatcc atttgatatg tctcagcaat
    26881 tgcgtcccat tttacacttt ctgtatcttt tcttgaaatc tgtttatcaa attcgatcat
    26941 cttaagacct ccagtcattt tcgtcgcttc tttttttgag ctatttacag tataatgata
    27001 aatgcgaaga gagggaagta gatgacttat aaaattggac aaataattga aggaaaagtg
    27061 acagggatac agccttatgg cgcattcgtt tctcttgata acgagacgca aggacttatt
    27121 catgtttcag aagtacaatc cggttatacg aaaaatatcc attcattgtt aaaagtcgga
    27181 cagccagtaa cagttcagat catcgacatt gatgaatatt ccaaaaaaat cagtttatct
    27241 ttaagaacgt tagaaaaaac aaatccagct gttccatatc gcagaaaaag gtatttcacg
    27301 aataaaaatc gtaagatcgg atttaccact attgcggagc agttacctat ttggatagaa
    27361 gaagcattat ctgaactaga acggaaagaa aataagaaat aaaaagtgta ttttttctga
    27421 aaatgtgcta gaatttttat gtaagccata gaacaagttg atcgaggtgg aaattttggc
    27481 aagcaaaaga gttgctggta caattatgtt gcatttggag gatggtacta aaaaattttt
    27541 ggtccattca attgatgaca agttggaatt tgcgttagcg gaactttctg aaggaaagac
    27601 tggtttagcg aatatactta atttcttaaa agaaatagtt catatcgatg ttagcaagat
    27661 tagtttgatg gaattgacaa atacacatat taaagaggaa aatgttccgt tgtttgtctt
    27721 tgaaactgaa caaaagaatc aaagagaaga attaggcgaa ggatatattt gggaagaacc
    27781 aggtcaaatg cgctctgttc ttggaactta cgaagtagag ggagtgcctt tcttctaaaa
    27841 tacagattaa gcgaacaaag aagtttgaat ttttttcaaa cttctttttt tattgtttgg
    27901 tccaaaaaat acaatgaaat gttctgctaa agatagtttt ttagtgcatt ttattttcaa
    27961 aatatgttga aaacggttgt ataacgaata ataataattg atcgaaaaat catttcacaa
    28021 agtaatgctt aatttatagt tgaccaagga agaaatacat gataaattta acagcgttga
    28081 ttctgaagtg gatgttatat cacatttcta aaaaatttaa aaaatagtta ttgacatgtt
    28141 actaacgaca tgataagata actgagtcgt t
//
LOCUS       DACCPY010000030        27782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCPY010000030 DACCPY010000000
VERSION     DACCPY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.030"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IVO69_001131"
     CDS             299..1960
                     /locus_tag="IVO69_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7399922.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IVO69_001132"
     CDS             complement(2030..2377)
                     /locus_tag="IVO69_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7399923.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IVO69_001133"
     CDS             2461..3402
                     /locus_tag="IVO69_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399924.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IVO69_001134"
     CDS             3403..4446
                     /locus_tag="IVO69_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7399925.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IVO69_001135"
     CDS             4452..5522
                     /locus_tag="IVO69_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399926.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IVO69_001136"
     CDS             5512..6465
                     /locus_tag="IVO69_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7399927.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IVO69_001137"
     CDS             6477..7487
                     /locus_tag="IVO69_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7399928.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IVO69_001138"
     CDS             complement(7772..8593)
                     /locus_tag="IVO69_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7399929.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IVO69_001139"
     CDS             complement(8690..8968)
                     /locus_tag="IVO69_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399930.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IVO69_001140"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IVO69_001140"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7399931.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IVO69_001141"
     CDS             complement(11724..12311)
                     /locus_tag="IVO69_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399932.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IVO69_001142"
     CDS             12486..13808
                     /locus_tag="IVO69_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7399933.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IVO69_001143"
     CDS             13963..14739
                     /locus_tag="IVO69_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7399934.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IVO69_001144"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IVO69_001144"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7399935.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IVO69_001145"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IVO69_001145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7399936.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IVO69_001146"
     CDS             16629..17258
                     /locus_tag="IVO69_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399937.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVO69_001147"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVO69_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7399938.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IVO69_001148"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IVO69_001148"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7399939.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IVO69_001149"
     CDS             19445..19981
                     /locus_tag="IVO69_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7399940.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IVO69_001150"
     CDS             complement(20021..21784)
                     /locus_tag="IVO69_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399941.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IVO69_001151"
     CDS             complement(21787..23502)
                     /locus_tag="IVO69_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7399942.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IVO69_001152"
     CDS             complement(24281..24505)
                     /locus_tag="IVO69_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399943.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IVO69_001153"
     CDS             24705..27473
                     /locus_tag="IVO69_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7399944.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..>27782
                     /locus_tag="IVO69_001154"
     CDS             27554..>27782
                     /locus_tag="IVO69_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7399945.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFL"
BASE COUNT         9055 a         5002 c         5588 g         8137 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 ac
//
LOCUS       DACCPY010000031        27379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCPY010000031 DACCPY010000000
VERSION     DACCPY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.031"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            254..484
                     /locus_tag="IVO69_001155"
     CDS             254..484
                     /locus_tag="IVO69_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7399946.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL"
     gene            484..825
                     /locus_tag="IVO69_001156"
     CDS             484..825
                     /locus_tag="IVO69_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7399947.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQLKSLDFKERRLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            1520..3601
                     /locus_tag="IVO69_001157"
     CDS             1520..3601
                     /locus_tag="IVO69_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7399948.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAERLAAEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFSLKLENDKQVRLETEIKEVKDGFLNRRERIFDLVLAIAYS
                     RNPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFFHYFPLAEIKKIEEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDYMITEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHIPFHLKEEALFENLYKHMAA
                     LIIRIKHAIQVMNPLKENIRTNYFPIHRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYQSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVDLVFSTFQLNYRNKPLLVIDPILTETNILVIEDFLKRNEELQRLKPNSHESTQLFT
                     QIIGVMEESGGRVDRIIYGKLEKLFEINNLEINKREIQPMLKDILTDSNIMIKEKAAT
                     WEETIERAAKPLVKENIVEERYVEAMIRAVNEYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NVEDFKQLLFQ"
     gene            3637..3939
                     /locus_tag="IVO69_001158"
     CDS             3637..3939
                     /locus_tag="IVO69_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7399949.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFSVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            3965..5254
                     /locus_tag="IVO69_001159"
     CDS             3965..5254
                     /locus_tag="IVO69_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7399950.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPILKAF"
     gene            5273..5680
                     /locus_tag="IVO69_001160"
     CDS             5273..5680
                     /locus_tag="IVO69_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7399951.1"
                     /translation="MFGFFKKKENEPKKRSIKEEKILSPEVTEKIQSEITQLNQEIST
                     TQEKPKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDVMRQIAKQVTIKGNK"
     gene            5703..6572
                     /locus_tag="IVO69_001161"
     CDS             5703..6572
                     /locus_tag="IVO69_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7399952.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKGTFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            6574..7194
                     /locus_tag="IVO69_001162"
     CDS             6574..7194
                     /locus_tag="IVO69_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7399953.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SVGFNNLEERAKQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKTRLQELAERY
                     GYQVMTIRLTADFEVLWQRRYKRDREPDRHLSYIMDHYHYGDILEDRSKGTNHITKEA
                     FKKIITDRKYDEFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            7391..7996
                     /locus_tag="IVO69_001163"
     CDS             7391..7996
                     /locus_tag="IVO69_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7399954.1"
                     /translation="MNNKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTVIEEGHPTPKVDVRAYIRKDGKILLIEDKRTREWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVESTQLQAVFDTNKQKDIPQLFQYYKLVFACTIHDEDAKFI
                     ENNETSDMGFFSIEELPKLSEKRTTKKQLLILENKNQLYCD"
     gene            8132..8584
                     /locus_tag="IVO69_001164"
     CDS             8132..8584
                     /locus_tag="IVO69_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7399955.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLKKVCKNVSEDWNFVKKGNKRIY"
     gene            8597..9117
                     /locus_tag="IVO69_001165"
                     /pseudo
     CDS             8597..9117
                     /locus_tag="IVO69_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9135..9845
                     /locus_tag="IVO69_001166"
     CDS             9135..9845
                     /locus_tag="IVO69_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399956.1"
                     /translation="MKKVIVDANFWEVFPEATIEILSVSGIDNHVTEENEEMYHQLLN
                     SAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKISGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVSIVRHTLTKDESEAEL"
     gene            10041..10409
                     /locus_tag="IVO69_001167"
     CDS             10041..10409
                     /locus_tag="IVO69_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7399957.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGIFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMDEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            10429..10653
                     /locus_tag="IVO69_001168"
     CDS             10429..10653
                     /locus_tag="IVO69_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399958.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRARNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKVKSHY"
     gene            10897..12210
                     /locus_tag="IVO69_001169"
     CDS             10897..12210
                     /locus_tag="IVO69_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7399959.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALSGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAMLLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGKLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            12538..14175
                     /locus_tag="IVO69_001170"
     CDS             12538..14175
                     /locus_tag="IVO69_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7399960.1"
                     /translation="MERKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDVIWLSPVYESPNDDNGYDISDYQAIMAEFGTMEEMEELIEEAKKRNIRIVMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGEEPNGLRSIFSGPAWQLDEQTGQYFL
                     HLYSKRQPDLNWKNKQVRQAIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSDPKRKELSMIFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDLYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDISEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEETPEHVFAYSRIYEDQRWLVVCNMSSEKQVFTVPRE
                     ALRIIVSNYPLKQLPDGKVELRPYEAFVMEVNKEKLI"
     gene            14323..14793
                     /locus_tag="IVO69_001171"
     CDS             14323..14793
                     /locus_tag="IVO69_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7399961.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRLLNKLADEAFRKTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FTKEVDGSWEELHQSYKAVLGNEASKELAEIMNEATDKLQKNKNFFKQISCMCN"
     gene            14859..15950
                     /locus_tag="IVO69_001172"
     CDS             14859..15950
                     /locus_tag="IVO69_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7399962.1"
                     /translation="MSQFDQSIILPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIVQIF
                     HGGRMTSRATLSGEQPVSASAVAAERPDAETPRALSENEIKETIQAFGEATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIDELLTTVFSTVEKYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRETKLDYLHVSLNAYDRRARSDKYSDQSI
                     LEAVHDTIQGKIPLIGVGGIRTRKDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            15980..16657
                     /locus_tag="IVO69_001173"
     CDS             15980..16657
                     /locus_tag="IVO69_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7399963.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYKDEDFRNRALGNTLRPDDVDAKDYAAVYYAGGH
                     GTMWDFPEATDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGADYQSGPAFSEVVQIDGRLLTGQNPQSAHQLGEV
                     VVEALKH"
     gene            17002..18297
                     /locus_tag="IVO69_001174"
     CDS             17002..18297
                     /locus_tag="IVO69_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7399964.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLIPTSEIYLIEKQSKLGWIPSGF
                     NLIVKGKVTSEKQLSWITKEELIDRYRIHVYTEKTVIGLEEKKVVLAGGDKLDFDRLI
                     LATGSNQNFRNVPAEARFIHPVKKIQNIAALQQKIMDAKKIAIIGAGQAGIEIAEGLA
                     SGKTQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGISLFLEEQVVSLSENETAVVET
                     ERRRENYDLVLLANHSRPNNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSATKDQGTYRPVGNQWFGYFLGSVGMTEE
                     ESIFYPQKIETGYAEYKLAAINQSKVKVKFLFDSEKKILGAQLQSRKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPNMVWSKVANTAYED"
     gene            18287..19747
                     /locus_tag="IVO69_001175"
     CDS             18287..19747
                     /locus_tag="IVO69_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399965.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKTSF
                     DQYLEDIELIGRMMGKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLGRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYETLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLEKLQETAMQQLPRKSTEDSALSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLNRELRGVSGIEIESYEEGKNYDLVITF
                     CQRNKQQNEYYLSEFASPYDIIRLKRRIEMLKKEKN"
     gene            19884..21380
                     /gene="glpK"
                     /locus_tag="IVO69_001176"
     CDS             19884..21380
                     /gene="glpK"
                     /locus_tag="IVO69_001176"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7399966.1"
                     /translation="MTEPTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQDILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEAAA
                     KRSTNEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            21394..23217
                     /gene="glpO"
                     /locus_tag="IVO69_001177"
     CDS             21394..23217
                     /gene="glpO"
                     /locus_tag="IVO69_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7399967.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQAAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPM
                     LLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LADIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVRELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMTPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAEYFGWTEEEKVQQTKRLEALIAESDLRELKGEK"
     gene            23218..23934
                     /locus_tag="IVO69_001178"
     CDS             23218..23934
                     /locus_tag="IVO69_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7399968.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            24240..26168
                     /locus_tag="IVO69_001179"
     CDS             24240..26168
                     /locus_tag="IVO69_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7399969.1"
                     /translation="MNNEMISQLQFDQIKKEIQARAIGNYSKKRISKMTTSSNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLVLSPADLIEYADFLRSSRMI
                     TKFFDKNQYQAPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQII
                     EKEIQSKLLKFLRHPKNKEMIQEAIIVQKGECYTIPIKASYKNKVDGTIIDESNKGTT
                     VFIEPTVVSKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEAIDLLIETMTVLDIIF
                     ARAKFSREINGITPKINKSEHIVIKQGRHPFLPDHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            26294..26848
                     /locus_tag="IVO69_001180"
     CDS             26294..26848
                     /locus_tag="IVO69_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7399970.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         9174 a         4621 c         5976 g         7608 t
ORIGIN      
        1 tagatgaact agcaagtttg attgcagact gtagacaaag gtatcccttg cctctttacg
       61 aagagtgtag gttgttactt ttgtctattt ttgtatagaa atagaaaaca tttgtctcca
      121 tttccaagct gctaattttt caaagtgcac catttctcaa aatgcttttg atcatttctc
      181 catctagttt acaaagggta cttttgcgtt acttagtgtt gtgtagtata cttttactga
      241 agaggtgatg atgatgcttg ttacaaaatc tagattacaa ggaagttctg tagtggttac
      301 tttaccttct gataacggaa agaaaccttc agaaaaccaa gaatatatcg ttgtatattc
      361 tgacgatggg acaattacgt tagttccaaa aattgaagat ccatttagtg gtggagaaga
      421 ggccgaatat tatgaaaaag acgaatggga agatttaact cctgaaggga gagaaaggtt
      481 atagtgtcag aagaaagaat ttatattcct aaaaaaggtg atattgtgtg gattgatttt
      541 gatccttcga taggaaaaga aattcaaaaa agacgaccag gactagtcgt ctctcgttat
      601 gagtttaatc gtaaaacaat gttttcggtc atatgtccga ttacttccac gataaagaat
      661 ttaccgacac gttactcatt accagaagag ctagatataa aaggacaagt acttatttct
      721 caattaaaat cacttgattt taaggaacga agactcaaaa aaatagaaaa cctgccattg
      781 caagacatgg ctaaaattga tcagattata caatatatgt tttaagcgtt gtaactaaac
      841 tctacaatat ttttatatgc taaacttaac aaaatttttt gacatttaaa aagaaaagtg
      901 acaataaaaa atagcatcta aatagcaatg acccccaaat ttaactcttg gggggacatt
      961 tcaaaaggga taagaaaaca aacattctcg tcgtatctga ctagtgaaaa agatggcagg
     1021 ccgattttat tcagccgatt atgctggatt gacaggaaat atttatgaat ggagctgtga
     1081 caaaaatttt gtcgcagctc ctttttctga ataaacggtg ttccagagat agcctatcgg
     1141 caataaggca cgattttgta aaatatggga agctattttt caaaatcgct tcttatcgct
     1201 caaggctgaa cctctccgtc acaacctttc cactaaatgg tattcaaaag cttacattta
     1261 cggtaataag ctcaaccaaa cgaaaaatag gaagcactat ttttgtttgg ctgttcttct
     1321 tacttgatgc aggacagctt tttcatgacc tcttttttgt aataataggc gtatgtcttt
     1381 ttcgttgaga agttgaaaaa gcatacggct tattttttgt ttgctatgct tactttcttt
     1441 ccacttcaag tggcagaaat atctactttt tattatgtaa gcggaaccat atacttggaa
     1501 tcagaaggag atcatttcta tgaaaacaag aacaattgaa atacttcaca gaatcatgac
     1561 agcccaatat ccagtaaaag cggaacgtct agcagctgaa ttcaatgtca gtcagcggac
     1621 gatccgtcag gaagttcttg aagcgaatat ctggttgagt agtaaaaagc ttccggaaat
     1681 cagaacaatt cgcaacaaag gtttttcgtt gaaactggag aatgacaagc aagttcgatt
     1741 agaaactgaa ataaaagagg tgaaagacgg ctttttgaat cggcgggaac gtatttttga
     1801 tttagtttta gccattgctt acagccgaaa cccgatccat ctaaacaaaa aagaagaaga
     1861 atttcaaatc tctaaaagta cgttagatga agatatgaga cgattacgag caacactggt
     1921 cagttatgga atcgagatcg tcagttatgg aaagcaggga ctggtttata aaggttcaga
     1981 acgatctatc cgtacgatga tctatgattt tatcaatcag aatctgggga tgaccgactt
     2041 ctttatgaat caagatgcac aactaacacc ggctcaaaaa atctttttcc attattttcc
     2101 tttggcggaa atcaaaaaaa tagaggagat ctattccaat aagttgttaa aacaagagga
     2161 tgatatttat aaaaagcaaa ttctgctttt tacattgatc tggatccaac gaatcaggaa
     2221 acatgaattc atctctgcca ttaattggaa aaatacagaa ataaaagaca gcagcttctc
     2281 tgcatttatt gactacatga tcacggaatt tcaattggac aatgttccac aagtagaaag
     2341 aaattatctt cgtttcacta tcgagacgtt caatgctaga gatatctcca attcgattga
     2401 gtgggtacaa gctcaactac taacgatcca attgatccaa tttgtagaga acgagacaca
     2461 catacctttt catctaaaag aagaagcttt attcgaaaat ctctataaac acatggctgc
     2521 actgatcata agaatcaagc atgctattca agtcatgaat ccattgaaag aaaacatccg
     2581 tacaaattat tttccaatcc accgcgcagt cactcatttt gttcctacga tcgaagaagt
     2641 aatcggcggc aagatactgg aggacgagat agcttttctt gtcatccact tttcgacgat
     2701 tgccagtgtg atcaagcgag acctgaatta tgtttatcaa tcagttgttg tctgtaatca
     2761 cggcatggct accgggaatc tgttagcaga aaatctgaaa gaaaaatttc ctcaaatcga
     2821 gattactgca gttttaagtt caaaagaaat cgaattagtg gataaactgg atgtcgatct
     2881 ggttttcagc acctttcaat tgaattatcg aaataagccg cttttagtta tcgatccaat
     2941 tttgactgag acgaatattt tagttataga agactttttg aaaagaaatg aagaactgca
     3001 gcggttaaaa cctaacagtc atgaatcaac gcagttattc actcaaataa tcggtgtcat
     3061 ggaagaaagt ggtggccgag tagatcgcat catctatggc aagttggaaa aattatttga
     3121 aataaacaat ctagaaatca acaaaaggga gatccagcct atgttaaaag atattttgac
     3181 ggacagcaat atcatgatca aagaaaaagc cgctacatgg gaggaaacga ttgaacgagc
     3241 tgctaaacca ttggtcaaag aaaacattgt tgaagaacgc tatgtcgaag cgatgattcg
     3301 cgcagtgaat gaatacgggc catatatcgt gattggcaaa cacatggcgc tagcccatgc
     3361 tcgaccagaa gatggggtca ataagttagg agtcagcgta gctacgattg agcaaccaat
     3421 cgatttcggt aatcctgaaa tggatccagt aaagattatt ttttgtttag cggcagtaga
     3481 ttcctactcg catctgaaca tcatgaaaga actgatcgaa ctgatcaaca atgaagagaa
     3541 gttggatcga ttgatcgagt gcacaaatgt tgaagatttc aaacaactgc tttttcagta
     3601 gttgagaaat aataaatgat acgggggaga acaaagatga acaaattaaa cattttattc
     3661 gtatgcggcg caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg
     3721 aacaaattag gagtagaagc caaactggac cattgtgata tttcttctgc agtgtccatg
     3781 aaaccagatg tgatcatcac agcagaaaat tttcgctcgc agttcgaaaa attctcagtc
     3841 gatcctaaaa cagcgatgat ctatttgaaa aatattgtct cgaaaaatga aattgaagaa
     3901 aaaatcaccc cagttctaca agataaggga gttatctaaa taaaaaagaa aagaggataa
     3961 aaacatgggc gttatcaatt ttatcatcga aaatattttg actcaggcat cgatcaccat
     4021 tgcattgatt gctatgttgg gtctactgct gcagaagaaa tcagcaggac aagtcatttc
     4081 tggcacgtta aagacactcc taggattcca agtgttgagt gccggttcga gtatcatcgt
     4141 tggaagttta acttatttcg ggaaaatttt tactgaagga tttcatatgc aaggaatcat
     4201 tccttctatt gagtctatca atggtcaagc aatgaatgat ttaggtttag gtagagatat
     4261 tgcactgacg ttcttagcga tttttgtatt caatattatt ttggctcgtt tcacaaaatg
     4321 gaagtacatt tttctaactg gtcaagccat tttatggatg gcgacgatga cgacggtctt
     4381 tggttatttc gctggtctgc gagggatcgt attgatctta gtcggtggtt ttatcggagc
     4441 ttgtttcgcc attgctatgc cagcagtagc gcaaccaatt atacgtaaga ttacaggatc
     4501 gaatgatatt gctttagggc atttttgtac gattggatac ttatttgaag ctggcgtggc
     4561 taaacttttt ggtgagaaag gtgaaaataa aaaatcgatt gaagagatca aattgcccac
     4621 gcatttcgag tttctgcaag atacgtattt gtctgtcatg gtcgtgatgg tcccactata
     4681 tatcatcact gttttgtttg caggtgaacc attcgcttct gaattatcag gagatcaaaa
     4741 ctatatcatg tttgcctttt tacaagccat ccaattcgta gtgggtgtgt atgtattgct
     4801 agctggcgta cgtctactgt taggtgaaat cgtgccggca tttcgaggga tagctatgaa
     4861 attagttcct gatgcgattc ctgccttaga ttgtccagtt ttctttccct atagcccaaa
     4921 tgctgttatt ttaggtttca ttacgacaac aatcggtacg attattgcca tgttcacttt
     4981 gccgatgttc ggcttagcga tgattttacc aggtatgcta acgaatttct ttgccggagg
     5041 aacagcgggg atatttggta acgcagttgg cggacgtcga ggagcgatca ttggcggtat
     5101 tgctcatggc ttctttatta cattattgcc tgctttgcta gtaacgatct ttaattcgat
     5161 gggattcatc aatgctacag caaccgatgt agatacagta gccgccgcac tcttatacgc
     5221 ttggatctta agcccaattt tgaaagcatt ttaattgaaa gtagggaaaa tgatgttcgg
     5281 attttttaag aaaaaagaaa atgaaccaaa aaaaagatcg ataaaagagg aaaaaatcct
     5341 ctcgccagaa gtgaccgaaa agattcaatc agagattaca caattaaacc aagagatttc
     5401 aacgacccaa gagaagccca aattagcgga actttacgaa caagttggac ttaaattttc
     5461 tgaattgtat gagaacgatc aagccatcca atatttagaa aagagcttgg aaaataaaca
     5521 gacgatcgga gatgggtata aaaaactgat gagcctctat aatcagaagc gtgcagatgc
     5581 tgctcgtgcg ggtgatgatc aagggatcga ttactacatg ggcaaaatgg atgtgatgcg
     5641 acaaattgca aaacaagtaa cgatcaaagg aaacaaataa aaagataagg tggcaataaa
     5701 aaatgtatac aacattgaaa gaagtaacaa aaacagcaga agagttgaat tttacagttg
     5761 gagcttttaa tacgcataac ctagaaatgc ttcctgccat gctaggcgca gcaaaagaaa
     5821 tgggttcgcc aatcattatt caaacaagtg tcgacaccgc aaaatatatc ggtatgcatg
     5881 tgttagtcaa tgtaatgaca gctattgcag aagaagaaat ggttgatgca gtcttgcact
     5941 tagaccacgc ccgagacttt gatgatatca aagaagcaat cgatagcgga tatacttctg
     6001 tcatgtatga cgggtcccat ctgcctttca aagaaaatat cttgaagacg aaagcagtgg
     6061 tagaatatgc ccatgcacac ggtgtttccg tagaaggaga acttgggacg atcggcggta
     6121 cagaagaagg aatccatgtc gctgaaaatg acaaagtata tacaagaccg gaagacgcag
     6181 tgaaatttgt agaagctaca ggtgtggatg cattagctat tgctatcggt acgaatcatg
     6241 gacaattcaa atcaaaaaca gaagtgaaca ttcccttgct gaaagaaatc gatgcagtag
     6301 tcgatgtccc cctcgttatc catggcggaa caggtgtgaa agaagaagat tatcccgaat
     6361 tgatcaataa cgggatccgc aaattcaatg ttggaacgga gcttttagtc aactggacaa
     6421 aagtggctaa aggaaccttt agtgaaacag aaatcaacaa gtctcttcgc cataatgtga
     6481 ttccagcaaa tgaagcagta aaagaaattg tcaagcataa gattggactg tttatgaact
     6541 tggaaagtcc aattaactta ggaaacaaat gagatgaaaa agtatttcat tcttctagct
     6601 ggtagtcctg gtacaggaaa aacatatctt atgaataaac tacgggaacg atttccagat
     6661 atgtatgcca tcacgctcga tgaaatcaag gaatattatg cagaatccgt cggttttaac
     6721 aacttggaag aacgggcgaa acaagaaagg acgaaagttt atcctttcta ttacaaagca
     6781 cttgatctgt atatggaagc aggaaagaaa gtgattgtgt cagagtatcc ttttagtgat
     6841 aagcaaaaaa ctcgactcca agaattagca gaaagatacg gatatcaggt aatgacgatt
     6901 cgcctaacag cagactttga agtgttatgg caacgtcggt acaaacgtga tcgtgaacca
     6961 gatcgtcatt tgagctatat catggatcat tatcactatg gcgacatatt ggaagatcga
     7021 tcgaaaggga cgaatcatat tacgaaagaa gcattcaaaa aaatcatcac tgaccgaaaa
     7081 tatgacgaat ttcagttagg aacactatat gaatttgatg taacagacta tactcaagta
     7141 gattacactc cactattgga taaattagaa aaagaaatca atgacaataa gtaacgaaga
     7201 aatggtttga tagtgacagg taataaaaaa gacatttggt ccccagagga gccaaatgtc
     7261 tttttgttct aaaatgtttt ttcatattct tttatttatt gccgatttcc ttgtgtttat
     7321 ttgccgtaat taggtcagtc ttctatactt gattacatag tcatacattt gctgaaaaga
     7381 agggaagaga atgaataaca agttggccca atacaaaaga ctgctcgcac tttctcaagc
     7441 tggtctgtat tatggaaaag atgtattcga taaagaacga tttgaagaga ttcggacgat
     7501 ctcattggaa ttgatcagtg aaataggaaa agaaactttc gaagaaataa aagcacttac
     7561 agttattgaa gaaggtcatc ccacaccaaa agtcgatgtt cgtgcctata tcagaaagga
     7621 cggcaagatc ctactaatcg aagataaacg gacgagagaa tggtcactgc caggcggatt
     7681 tgccgaaata ggtttgtccc ctgaagaaaa cgtgcggaaa gaagtctatg aagaaacggg
     7741 tttaacagta gaatctactc agctgcaagc ggtttttgat acgaataaac aaaaagatat
     7801 tccgcagctt tttcagtatt ataagcttgt ttttgcttgt acgatccacg acgaagatgc
     7861 aaaatttatt gagaacaatg aaacttccga catgggattt tttagtatag aagagctgcc
     7921 aaagctatca gaaaaacgaa caacaaagaa gcagttgctt attttagaaa acaaaaatca
     7981 gctctattgc gattgagttt tttcgtaaaa gaagaaagct gtttactctg cgagttctct
     8041 aaaaaagaac tcgttctttt attaaaaaat agttgcattt gcaacaaaaa gaggtaaact
     8101 agtcttacaa acaaaataaa ggtggggtga aatggctgaa attcttcgag acattggaat
     8161 gattgcccga gcgttggatt ctatcagcaa tatcgaattc aaagaagttg atttaacaag
     8221 agggcagtat ctttatttag tacgtatttg cgaaaatcca ggaatcatcc aagaaaaact
     8281 agcagaaatg atcaaagtag atcgtacaac gactgcacga gcaatcaaga aattagaatc
     8341 caatgggatg atcgaacgtt tggaagataa agaaaataaa aaaatcaaga aactttatcc
     8401 gactaaaaaa ggagcagaaa tctatccttt catcatccga gaaaacaact actctaatgc
     8461 agtcgcatta aatggtctgt ctgacgagga agcaaaacaa ttggaatatc ttttaaagaa
     8521 agtatgcaag aatgttagcg aagattggaa ttttgtcaaa aaaggtaata aaaggatcta
     8581 ttgaaaggaa gaagccgtga ataaatacga aattcgagaa atgacgcctt cagatgtaga
     8641 cttactacaa caaatcagta tcgaaacatt tacagataca tttggaaaag ataattcgcc
     8701 ggagcattta gctgcttact tgagacaagc ttatgcgcca gaaaaactta cagaagagct
     8761 aaaagagaaa gaaactgatt tttactagta agtatggacc aagagcctgc gggttacctg
     8821 aaactaaata taggaacgag tcaatctgag gaaatggatg atcatttttt agaaatcgag
     8881 cgaatctatg tacgaaaaaa atttaaacat caaggtattg gtacattttt actagataca
     8941 gctatcaaaa aagcaaaaga aaagcaaaaa acagccattt ggctcggtgt gtgggaacac
     9001 aacgatgctg cgctcgcttt ctatcgcaaa agaggattta ccaagtacag tgaacatgtt
     9061 ttttatatgg gagatgatcc ccaaacagac tatctattca tcaagcaatt attctaatca
     9121 agaggtgtga acatatgaaa aaagtaatag tggatgccaa tttttgggaa gtatttcctg
     9181 aagcaaccat cgaaatctta tccgtgagtg gtatcgataa tcacgtaaca gaagaaaatg
     9241 aagaaatgta tcatcaatta ttgaatagcg cggcaaaaga agcaagaaat taccttacgg
     9301 aagaaacatt cagccaaaac gaagtcattg ctcagtggcg tcaggctttt accacattca
     9361 agactaaaaa aggtgcacgt tcttctattg aagctttact gaaacgtgca aatcaagaac
     9421 gggaattttt cccaatcaat ccgcttgtag atatttataa tagtatttcc ttgtcttttg
     9481 gcgtaccttg cggcggagaa gatatcgaaa agatttcagg aaatctgcat ctaggaaaag
     9541 cccaaggtgg cgaatcattc ctgcctttag gtgcagattc cgatgctccg gcacttccgg
     9601 aagaaatcat ctactatgat gaacaaggag caatctgtcg ctgtctcaac tggcgtgaag
     9661 cccaaagaac gatgctgacg gaagaaacgc aagcagcggt gctggtcatt gaatccatca
     9721 atgctgaaca agcggcacgc ggcgcacagg caatcgaagc gttgaaggaa aaaatcgatc
     9781 aaacatttgg cgtgtccatt gtcagacaca cactaaccaa agatgaatca gaagcagaat
     9841 tataaaagaa tcgaaaaaag aagagcggca ttcattgccg ttcttttttt gtaaaaaaag
     9901 tttgtggagg aaagcaagtc tagaaactat tgctttattt ttcttgaata ggatcaaaca
     9961 caaggtgttc gcgctttcgt gatataatgg aatcagttta tagacaggaa agaaaatcaa
    10021 ctagaaagaa gggtcaaacg atgatacaaa aaacacgtgt gatgctttat gtcaatgacg
    10081 tagagatgat ttgccgattc tttgtagaaa aaattggtgc agaaatttca gaaacgatcg
    10141 aattgccaga agaatttaag agtattgtgc tgagtatctc aaaagaatta gaattaggta
    10201 tatttccaaa agtatttgtg cagaagtttt caccagaagt attgggaccg ccaccttctt
    10261 tagtcttttt tacggatgaa tttgaaacta tttacgagaa catggacgaa cctggagaaa
    10321 tcacagacaa caatggcgtc ttgactttca atttttcgga ccctgaaggc aattattttg
    10381 ttataggaaa agctgagtcg tctgattgaa taaaaaggag gaaaaacagt ggatctaatg
    10441 ggaaggctag aaggttattt gaagtatcgg cgagcaaaac atagtgagaa gcttcgagca
    10501 aggaatctca aatcagggaa aaagaagaca aaaggctggg gaaaaatggt cgatacaggt
    10561 tttggcggac taaataacct aaatcctgtc gatacaaaag aattcatcaa agaagagcag
    10621 aaacgaaatg gtaaagtcaa atcacactat tgattttgat actagaatga agaaactttt
    10681 ttaacacaat aaagaaattc ttttgatcca aagtgaatgt tcatggactt tgagataaag
    10741 ggatttgaac agatggaacg ttcggatttt gcttaaaatc cgaatttttt gtttacttat
    10801 gttttgtttt agtatatgat aaaacacgtt gttttattaa tcaatcgaaa ttttaacgga
    10861 ctttatttat aatgttcgga tttaggggga tggaagatga aggaaaaaat actttcctac
    10921 ttcgaaatag aaaaattgaa taccacggta aaacgtgagg tactggcagg atttacgaca
    10981 tttatttcaa tggcctatat cttatttgta aatccaactg tattaggcgc ttctggaatg
    11041 gatgaaggag cggtgttcac tgcgactgca ttagccagtg caatcggctg tattttgatg
    11101 ggggtattag cccgttatcc aattggaacg gcacctgctc ttgggatcaa tgctttcttt
    11161 gcttattctg tttgtttagg gatggggatc ccatgggaaa cagcattgtc cggcgtattc
    11221 gttgcttctt tgatctttat tttgattaca atcttcaaac tgcgggaaat gatcattgat
    11281 gcgatcccag cagatttgaa atatgcgatt tctggtggta tcggtctatt tattgctttc
    11341 cttggtctga gcgaaggtgg gatcatcgtt tcgaatgaat ctacattagt aggattagga
    11401 tcactcaacg ttggttctac ttggttgacg atttttggat tagtcgtcac ggctatgttg
    11461 cttgttcgac gtgtaccagg tggcattttc atcgggatgg cagctacaac gattttaggt
    11521 ctggtcacag gtttgattcc aatgccttct gaaattgttg ctgcagcacc aagtttgaaa
    11581 ccaacatttt tagttgcgtt aaaacatgta ggggacatca attctcttca aatgtgggtc
    11641 gtagtactaa cattcttgct tgtgactttc tttgatacag caggaacttt agttggatta
    11701 gcaaatcaag caggctttat gaaagacaac aagatgcctc gtgtagggaa agctttagct
    11761 tctgattcaa cagctatgct tgcgggctcc ttattaggta cttctcctgt tggtgcattc
    11821 gtagaatcat cagctgggat tgctgttgga ggacgctcag gaatcacagc tattactaca
    11881 gggattttct tcttgttagg tttgttcttt tctccattac tgtcagttgt tacttcacaa
    11941 gtaactgcac cagcattgat catggtaggt gttttgatgg ctcaatcatt acgtcaaatc
    12001 gaatggggaa aattagaaat tgcgatccct tccttcttga tcttgattgg catgccttta
    12061 acttatagta tttcagatgg gattgcatta ggttttattt tctatccaat tacgatgtta
    12121 gcggctaaaa gaggaaaaga agtatctcca atcatgtatg gtctgttctt tgtattcgtg
    12181 ggattcatgt ggatattgaa tgtgaaataa agaggttgtg aaatcagcgt tctggcctga
    12241 gtattagaga aaaaaacgaa aaaatagttc ctcctatttt gccgtttttg gagctaatac
    12301 cgagggacag ctgagtgaac accgtttata aagaggttgt gaaatcagcg ttcttccctg
    12361 catcaatcaa tcctttcaaa aaacagttga ggaaatttcc tcagctgttt ttttgtttta
    12421 ttctgttacc gataacaagg tgcaatgact atttgaaagc gttacaattt ttggtagaag
    12481 aaactaaact agatgattaa gcgaaaaata gaaacatcca tttataggag gaaaatcatg
    12541 gaacgaaaat ggtggcacca ttcagttgtt tatcaaatct atccaagaag ttttcaagat
    12601 tcaaatcaag atgggattgg agacttgcga gggattattt ccagattgga ttatctggca
    12661 tatttgggca ttgatgtgat ttggctttcg ccggtgtacg agtctccaaa tgatgacaat
    12721 ggctatgata tcagcgatta tcaagcaatc atggcagaat tcggtacgat ggaagaaatg
    12781 gaggaactga ttgaagaagc aaagaaaaga aacatccgta tcgttatgga tctggtggtg
    12841 aatcatactt ctgatgaaca cccgtggttc attgaggcac gtaaaaataa agaaaatcct
    12901 tatcgtgact actatatttg gcgtgatcca gtcaatggag aggagcctaa tggattgcgc
    12961 tcgatattca gtggacctgc ttggcagttg gatgagcaaa cagggcaata ttttctgcac
    13021 ttgtatagca agcgtcaacc tgatttgaat tggaaaaaca aacaggttcg ccaagcgatt
    13081 tatcaaatga tgaatttctg gatcgacaaa ggtattggtg gctttcgtat ggacgtgatc
    13141 gatatgattg gcaaagaacc ggataaagaa atcacaagta atggacctaa actgcatgag
    13201 tatcttcagg aaatgaatca agcaactttt ggcgacaaaa atctgatgac cgttggcgaa
    13261 acatggggag caactaccga gattgctaaa ctttattccg atcctaaaag aaaggaatta
    13321 tccatgatct tccaattcga acatatagga ttagatcagc aagaaggcaa agacaaatgg
    13381 gatttgaaac ctttaagtat ctctgaactg aaaaaagtat tatcgaaatg gcaaacttct
    13441 ttgggtgatg aaggatggaa tagtttattt tggaataatc atgatctgcc tcgaatcgtc
    13501 tcacgttggg gaaatgacga tttgtatcga aaagaaagtg ccaagatgtt tgctatcttg
    13561 cttcatatga tgaaaggcac tccttatatt tatcaaggag aagaattagg catgacgaat
    13621 tgcccaattg atgatatctc ggaagcaaaa gatattgaga caatcaatat gtataacgaa
    13681 aggatttctt ctggatttac aaaagaggaa atcttggaat caatcaatgc gaaaggaaga
    13741 gacaatgcta ggacgcctat gcaatggaac gcccgtgaac atgctgggtt tactactgga
    13801 attccatggc ttcgagtcaa cccaaattat aaagaaatca atgcagaaga agcgttagca
    13861 gatcaagatt ctgtttttta tatgtacaag aaattgatcg atttgcgtag aaaacatgac
    13921 atcatcgtct ggggagacta tgaattagta gaagagacgc cagagcatgt ctttgcctat
    13981 tctcgcatat atgaagacca gcgatggctc gttgtttgca atatgtccag tgaaaaacag
    14041 gtgttcactg ttccacgtga agctttaagg attattgtgt caaattatcc tctgaaacag
    14101 ttaccagacg gcaaagtcga gttacgacca tatgaagcat ttgtgatgga agtaaataaa
    14161 gaaaagctta tttgattgaa acgtattacc gagaggttgt gacacatttt ttgtcacaac
    14221 ttctttttct caaaactaaa cagaggaggc ggtcattact ccgtgttttt tgacaaaaaa
    14281 taaaaatcat actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa
    14341 aaagcgttgg atgaatgtct tttctttagc gtgaaaaagc tagatcgcct attgaataaa
    14401 ttagcggatg aagcgtttag aaaaacaggt ctggcaccaa cttatggatt tattttattg
    14461 atattaaaag aaaaagatgg tataccgcaa aaagatattg cacaaatgct ctattctgcg
    14521 ccttcaacaa tcgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt
    14581 agcgaagggc gcttgtcatt agtttatctt acagaacaag gacgccaatt tactaaggaa
    14641 gtcgatggta gctgggaaga actccatcag tcgtacaaag cagtcttggg aaatgaagca
    14701 agtaaagaat tagccgagat aatgaatgaa gcaacagata agttacaaaa aaataaaaat
    14761 ttttttaagc aaattagttg catgtgcaac taaccgagtg gtttaatgat aaatgattct
    14821 cgataggtta atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa
    14881 ttattttacc ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac
    14941 aactcagctt cttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt
    15001 ctaaaggact tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct
    15061 ggccaggcga actgagcatc gctcacgatg aaatgattcc tcggttaagt gaactagcta
    15121 aagcaatcca aggagaaggt gcaaaagcga ttgttcagat tttccacggt ggacgaatga
    15181 ctagtcgagc tactctatcc ggagaacagc ctgtatctgc aagtgcagtt gcagcggaac
    15241 gacctgatgc agaaacaccg cgagcattaa gtgaaaatga aatcaaagaa acaatccaag
    15301 catttggcga agccacacgc agagcgattg aatcaggatt tgatggtatc gagttgcatg
    15361 gagcaaatac gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc
    15421 aatggggcgg tagcttagaa aatcgctatc gttttattga cgaattatta acaacggttt
    15481 tctcaacggt tgaaaaatat gcaaaaaaac cattcatcgt agggtatcgt ttctcgccag
    15541 aagaatatga aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac
    15601 gtgagacaaa attagattat ctgcatgttt cattaaatgc gtatgatcga agagcccgtt
    15661 ctgataaata cagcgatcag tcgattcttg aagctgtcca tgacacgatc caaggaaaga
    15721 tccctttaat tggtgtcgga ggcatccgta ctcgcaaaga cgtagagcaa gtactgaaat
    15781 atgcagaact tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat
    15841 tattggaaaa ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc
    15901 tttatttgcc aagtccatta tataatttct tgaaagcacg ttatcaataa ctcaattttt
    15961 atagaaagag gatggaaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga
    16021 aaagatcgag cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt
    16081 agtaaaaaat gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga
    16141 accgcaaagt gttttgaata tggatgaaat cgattgggcc tattataaag atgaagattt
    16201 cagaaatcgg gccttgggaa ataccttacg tcctgatgat gtggatgcaa aagattatgc
    16261 agcagtttat tatgctggtg gccacggaac aatgtgggat tttccagaag caacagatct
    16321 tgggaaagtt gcttcacaaa tctatcgaaa tgaaggactg gtctcagctg tttgtcacgg
    16381 tgttgtcgga ttgttgccag ttacagatga atctggaaag tctattttag ctggacgcac
    16441 agtgactgga ttcagtaatg aagaagagga agccaatggt acaacgaatg aagtaccatt
    16501 cttagcagaa gatgctttga aagaaaaagg agctgactat cagtcaggtc cagctttttc
    16561 agaagtcgtt caaatcgatg gacgtcttct tacagggcaa aaccctcaat ctgctcatca
    16621 attgggcgaa gtagtggttg aagcattaaa gcattaaaaa ttgtcagcat aaaaatgttt
    16681 tgcctattct aactattaca ttacacacgc taaaacacgt cttttcgttc ttttctgttt
    16741 cctatttgga cagaggtacg caaaaggcgg gttttttgcc tttctaagag tacatttttt
    16801 gaaacatctc ttcagaaatg atactcttca attaggtcaa ctaatttatt actgagaagc
    16861 agacctagtc acacggtagg atggacagag tgttttctgc tacgctgcta tggatcatag
    16921 gaatgagtaa acatggttta agaacattgt gatttcagcc catgttggca gtcgcacact
    16981 taataagaaa gaggaataca gatgaacatt gtaattgtcg gcgggtcgtt tgcagggatt
    17041 catgcggcta tttatcttcg ccaattgata ccaacaagtg aaatatattt gattgaaaag
    17101 caatcgaagt tagggtggat tcccagcggg ttcaatctga tagtaaaagg aaaggtcacc
    17161 agtgaaaaac aactttcttg gatcaccaaa gaagagctga tcgaccgcta tcgtatccat
    17221 gtttacacag aaaagacggt aataggattg gaagaaaaga aagtcgtttt agcaggcgga
    17281 gacaaactgg attttgatcg tttgattttg gcgactggtt caaatcaaaa ttttcgaaat
    17341 gttcctgcag aagcaaggtt cattcaccca gttaaaaaga tacaaaacat agctgctctt
    17401 cagcaaaaga tcatggatgc aaaaaaaatt gccatcattg gagcaggtca agcagggatc
    17461 gaaatagctg aaggcttagc atcaggaaaa acacaaatcc atctttatga aagccgaaag
    17521 tcgattcttt ttcgttacct agaccctgaa atgacggagc cgttagtaaa ggaaatgaaa
    17581 aatcaaggga tatcgctttt cttagaagag caagtcgtat ctttgtccga aaatgaaacc
    17641 gctgtagtag agacggaaag aagaagggaa aactatgatc ttgttttgct ggctaaccat
    17701 tctcgcccca acaatcagat gtgggaagaa caattaacat tgaatgatga tggaacgatc
    17761 tgggtagatg actatttgca aacgtctcaa aaagacgtct atgcaattgg cgatgcaatc
    17821 caagtcactt ttcgcccaac aaatgaaaaa atgtacgttt ctctagtaaa caacgctgtc
    17881 cgtacagcgc gaaatgtcag caagacaatt agtggttctg cgacaaagga tcagggaacc
    17941 tatcgaccag tcggcaatca gtggtttggc tatttcttag gaagcgtagg tatgacagaa
    18001 gaagaaagca ttttttatcc ccaaaaaatt gaaacagggt acgcggaata caagcttgca
    18061 gcaataaacc agagcaaagt caaagtcaaa ttcctatttg attctgaaaa aaagattctt
    18121 ggagcacagc tccaaagcag aaaagaaatg ttccatcttc ttgaccaact gacaatcgca
    18181 gtagaagaag gctggacgtt cgaacagtta gaagaacatg agctgttttt ccagccagaa
    18241 tatcgtgtac ctaatatggt gtggtcaaag gtggcgaata ctgcctatga agattgaagc
    18301 attattagaa agaaaagagc agttacagat cctgattttg cgaaaccttg ttctgcaagg
    18361 gggaacagcc tctatcaatg gattgagaga gcatgtccaa ctgtcaaaaa cctcttttga
    18421 tcaatatttg gaagatattg aattgattgg acggatgatg ggaaaaaaag tagaagtaca
    18481 gcgaaacgag tatcaggcgc tgcttgtgtt ggacgaagat gtcagtttgg aaaaaattct
    18541 tttgttttta ttacaggaaa gcttgaagtt taaaatgctt gtctatttat tagaacacca
    18601 gcaagtatcc atcgttcgat tagcgacagc gtttaatatc agtgagtctt ctgtttttcg
    18661 aaagatcaaa gaattgaacc agctcttaga ggaatttgga ttacaaatca aaaacggaca
    18721 attatacgga gaagagctac agatccgata tttttattat gaattattcc aatatatccc
    18781 ggaagatcaa cgaccgcttt ttttgcaaaa tacaccagaa aaaagaccat tcattctagg
    18841 attgggccgc gtattagaaa cgacgtttac ggcatcagca gaagcacaga ttgcttgctg
    18901 gctcgggatc acgaaaaaaa gactattgaa tgaaaaaagt acgtacgaaa ccttgaaaga
    18961 aaagaaatta ctctatcagt cggaccggct ttatcaagca atcgatccaa tcatcgtgat
    19021 gcatcttagt cgaacagcag cagaactgaa tgtatacgaa acaatgatgt tttacagctt
    19081 ttttgtttct ttttcgatag tcgacgaaga aatattttat caatatgatt tgacacggag
    19141 caaaaagtta cccaccgctg ttttggatac ttatattcgg gaaacgatgc tatggcatta
    19201 tcgtcctcgt cgtttgaaaa tcaaagagga aaaagcagta ggctaccaaa tttcacaaat
    19261 caacaatgaa tggtttttct ttgttggaaa gatcgaagtc tacgagcgtg atcggctttt
    19321 ggagcagcag caaaaaatgc ttgggcattc cttaacacag ttattggaga aactacagga
    19381 aacagcgatg cagcaattgc caaggaaaag caccgaagac agtgcactta gttatctgat
    19441 gatccagtat gccaatgttc ttttgatgat cgatttttat attgcgaaac cagtagtgat
    19501 agggatcgat ttagaaagtt tgcctattta ccgtattgct ttccaacagt atctgaacag
    19561 agaactgaga ggagtcagcg gtattgagat tgaaagttat gaagaaggaa aaaactatga
    19621 tttggtcata acgttttgtc agagaaacaa gcaacagaat gaatattatc tatcagaatt
    19681 tgcgtcgcct tatgacatta tccgattgaa aagaagaata gaaatgttga aaaaagaaaa
    19741 gaattgatca aaaactagca tcataataaa aaactgtcaa aaaaaagaaa aaaagaataa
    19801 aattttgaca gtttcttttt ttaaatgagt agtatgataa aagcagttaa agaaagcgtt
    19861 tgcagataat ggagggaatc acaatgacag aaccaacgta tatcatggcg attgaccaag
    19921 gtacaactag ctcacgagca attatctttg acaagaaagg aagacatatc ggcagttctc
    19981 aaaaagagtt cacgcaatat ttcccgcaag aaagctgggt tgaacatgat gcaaatgaaa
    20041 tctggaattc tgtacagtca gttattgccg gagctttcat cgaatcaggg atcaaaccaa
    20101 accagattgc tgggattggc ataacgaacc agcgtgaaac aacagtaatc tgggaaaaag
    20161 atacaggacg tccaatctat catgcagttg tttggcaatc cagacaatca gctggaattg
    20221 cagataactt aaaaaatgaa ggatatcaag aatttttcca cgaaaaaaca ggactagtga
    20281 tcgatgccta tttctcagca acgaagattc gctggattct tgatcatgta gaaggtgcgc
    20341 aagaacgcgc tgaaaaaggc gagttaatgt ttggaacgat tgattcgtgg ttagtatgga
    20401 aactgaccga tggacaagca catgttactg attactcgaa tgccagtcgg acgatgttgt
    20461 tcaatatcca tcagctagat tgggatcagg atattttgga tttattgaat attcctcgag
    20521 tgatgttacc aaaagttact tctaactcag aagtttatgg ttatacgcaa ggttatcatt
    20581 tttatggaag cgaagcacca atttcaggga tggcaggaga ccagcaagct gcattattcg
    20641 gacaaatggc attcgaacca ggtatggtca agaatactta tggtacaggt tcatttattg
    20701 tcatgaatac cggtgaaaaa ccgcaattat ccaaaaataa tttattgacg acaattggtt
    20761 acggtataaa tggaaaggtt tactatgctt tagaaggaag tatttttgtg gctggttctt
    20821 cgattcaatg gctgagagat ggtcttcaaa tgttgcaaaa ggctagtgat tcggaagccg
    20881 cggcaaaacg ctccacaaac gaagatgagg tctatgttgt tcctgcattt gttggactgg
    20941 gtgctcctta ttgggatcag gcagcacgag gggctatgtt tggtttgaca cgtggaacga
    21001 caaaagaaga tatcattaaa gctactttgc agtcgattgc ctatcaagtg agagatatca
    21061 tcgatacgat gcaagatgac actggaatca agattcctgt attgaaagtg gatggcggtg
    21121 cagcaaataa tgaatatctg atgcaattcc aaacagatat cttgaatgta ccgatccaac
    21181 gagcagaaaa cttggaaaca acagcactag gggcagcatt tttagcagga ttagctgtcg
    21241 gctattggaa agatacagac gagattcgtg aattttatga agctggcaag ctatttgaag
    21301 ttcaaatgga ggaagagcgc cgagaaaaac tttacaatgg ctggaaaaaa gcagtaaaag
    21361 ctacccaagc atttgaataa ggaggaaatc catatgtttt caaacaagac aagacaagat
    21421 agcattcaaa aaatgcagca agaagaattg gatctgttga tcatcggtgg cggaatcact
    21481 ggtgccggtg tggcagtcca ggcagcagca tcaggaatca aaacaggatt gatcgaaatg
    21541 caagattttg cagaagggac gtcctctcgc tcgaccaaac ttgtgcatgg cggtattcgt
    21601 tatctgaaaa catttgatgt ggaagtagta gctgacacag ttggtgaacg tgcagtcgta
    21661 caaggtattg ccccacacat cccaaaacca gatccaatgc ttttaccaat ctatgaagat
    21721 gaaggagcaa caaccttcaa tatgttctct gtcaaagtag caatggacct ttacgacaaa
    21781 ctggcaaatg tgacaggaac taaatatgag aactataccc tgacaccaga agaagtattg
    21841 gaaagagaac catttttgaa aaaagaaggg ctaaaaggtg caggtgttta tctggatttc
    21901 cgcaacaatg atgcccgttt agtgatcgat aatatcaaaa aggctgcaga agatggggct
    21961 tatctagtaa gtaaaatgaa agcggttggc tttttatatg agggcgatca aatcgttggt
    22021 gtcaaagctc gtgatctgct gacagatgaa gtgatagaga tcaaagcaaa attagtgatc
    22081 aatacgagtg gtccttgggt agataaagta cggaacttga attttacgcg tccagtctct
    22141 cctaaaatgc gtccaaccaa aggaatccat ttagtcgtag atgcgaaaaa actgcctgta
    22201 ccgcaaccca catacttcga tacaggaaaa caagatgggc ggatggtttt tgctatccca
    22261 agagaaaaca aaacttactt tggtacgaca gatacggatt accaaggaga ctttacggat
    22321 ccaaaagtca cacaagaaga cgtggattat ctattggatg taatcaacca tcgctatcca
    22381 gaagcaaata tcacattggc agatatcgaa gcaagctggg cagggcttcg tccactattg
    22441 attggtaatt ctggttctga ttataatggc ggagataacg gatcgatttc agacaagagc
    22501 ttcaataaag tggttgatac agtaagtgaa tataaggaaa ataaagtttc tcgtgctgaa
    22561 gtagaagatg tgttgaacca tttggaaaac agccgcgacg aaaaagcacc ttctacgatt
    22621 tccagaggta gttctttaga aagagaacca gatggcttgt tgactttatc aggtgggaaa
    22681 atcactgatt accgtaagat ggcagaagga gctttacgat tgattcgtca gctgttaaaa
    22741 gaagaatacg gaatagagac gaaagaaatc gactctaaaa aatatcagat ttcaggtgga
    22801 aacttcgatc caacgaaatt agaagaaaca gtgagggaat tagcaaaaga aggagtagca
    22861 gccggtttag aggaagaaga tgctacttat atcgctgatt tttacgggac taatgctcga
    22921 cgtatctttg aattagcaaa agaaatgaca ccttatcctg gtttgagtct cgctgagtca
    22981 gctcggttac gttatggctt agaagaagaa atggttttag ccccaggtga ttatctcatt
    23041 cgtcggacga atcatctgtt gtttgaacga gaccagctgg atgagatcaa gcaacctgtg
    23101 atcgatgcaa ttgctgagta ttttgggtgg acagaagaag agaaggtgca acagactaaa
    23161 cgtttagaag cattgatcgc agaatcagat ctgcgggaac taaaggggga gaaataaatg
    23221 aacagtgata tgactcaaat catgggggag tttattggga cattgatcct agttttgctt
    23281 ggagatggtg tgtgtgcggc tgtgaatcta aacaaaagta aagcacaagc ttctggctgg
    23341 attgtcattg cgtttggttg gggactcgct gtaacaatgg ctgtctatat ttctggattc
    23401 atgggcccag ctcatttgaa tcctgcagtc tctctagcta tggcgatgac aggggcaatt
    23461 agttggaatc ttgtcgttcc tttcatcatt gcgcaagttt taggagcttt tgcaggggcc
    23521 attcttgttt ggctttctta tttgccacat tggaatgcaa cgaaagatga aagtgcgatt
    23581 ttgggtacgt ttgcaacagg tcctgctata cggaactatc cagcaaatgt aatcacagaa
    23641 ttaattggta cttttgtatt agttcttggg ttgctagctt ttggacaaaa cgaatttgca
    23701 cctggaacga acgtattcgc tgtaggtgga ttgatcttag ccatcggtct ttcattagga
    23761 ggaccaacag gatacgcaat caaccctgct cgggactttg gtcctcgatt agcacatgcg
    23821 gtattgccaa tagctaataa aggaacttca gattgggcat attcatgggt accaatcgca
    23881 ggtccaatga tcggagcaat tatcgcggta ggtgtctatt cattaatggt gtaaatctat
    23941 aaaacatact aaggtttgac aatcaaaagg cgtcttgcta tagttaagtg gaaaataaat
    24001 aagacctaga agaatgggga atgttttgtg agtgtagctg gtatctaatc agtaagtcga
    24061 atagccaata aacaaggttt atttggtgtg cttatgaaac ggtacctgtc aggaaaacaa
    24121 tatctttgta ttctaataag gataggacat gactttttta agctgtgttc tatcctttta
    24181 tttatctcta ggcccttatt tattttggga caggtatctc ttcaagaaaa ggagagagta
    24241 tgaacaacga aatgattagt caattacaat ttgatcagat caaaaaggaa atccaagcaa
    24301 gagcaatcgg aaactatagc aaaaaacgaa tcagtaagat gacgacctcg tcaaacctac
    24361 aaaccgtctt ggatcgtcaa gaagaaacaa gagaagcacg cttgatctta gaaagtagtc
    24421 agcatgttcc atttatggga ttgccgagga ttgatgcact gacagaacaa gtcaaaaaag
    24481 gattggttct gtcgcccgct gatttgatcg aatatgcgga ttttctaaga agcagtcgga
    24541 tgatcacaaa gttttttgat aagaatcaat accaggcacc tttgttattt gcttatagca
    24601 agcatcttcc tgatttgata aatgtagaag agttgattga ccaaaaaata aaaaataata
    24661 aagtcagtga cgatgcttca cgtaatttgc gtaaagtacg caaacaatta caaataatag
    24721 aaaaagaaat ccagtcaaaa ttgctgaaat tcttgcgtca tccgaaaaac aaagaaatga
    24781 tccaagaagc aataatcgtt caaaaaggtg aatgctatac gattcctatc aaagccagct
    24841 ataaaaataa agtagatggg acgatcatcg atgaatcgaa caaaggaacg acggttttta
    24901 ttgaaccgac tgtggtttct aaactgaatg aacactatca gttgttaaaa gctgaagaaa
    24961 tcagtgaaga atatcaagtt ctggctgcat tgactggtgc tattgcagaa aatgaagaag
    25021 caatcgattt attgatcgaa acgatgacgg tattagacat catttttgct cgggcaaaat
    25081 tcagtcgaga aatcaatggg atcacgccta aaatcaataa atcagaacat atcgtcatca
    25141 aacaaggcag acatccattt ttacctgatc atgcggtacc attggatgtt gaaatcggta
    25201 aagactatcg aggcttgatt attaccggag caaatgccgg aggcaaaaca gtcgtgttga
    25261 agacagtcgg tttattgact ttgatggcga tgttcggtat gcaagttccg gcaaaagaag
    25321 ggacagagct tgctgtcttt gatgagattt tcgtcgatgt cggtgaccac caaaatttag
    25381 aaaatgcgtt gagtacattt tctggtcata tgcaaaacat cgctgctatt ttgaaaaaga
    25441 taaaaaggaa tacgttggta cttttagatg agattggcag tggaacagag ccaaatgaag
    25501 gggctgcact agcgatcgca atcatggaat ctatgtatga gcaaggggca ttgatcattg
    25561 ccacgaccca ttatggagaa atcaagaaat tcgccggaga tcatgaagat tttgtgccgg
    25621 ctgctatggc tttcgatcgg gaagcacttc gtcctaaata tcagttgcgg gttggagaaa
    25681 caggagaaag ccaagctttg tggattgctc ataaaatgga tatgtcgatg aaattgatcc
    25741 atcaagcaga gcgttacttg gcggaaaaag cctatcggac acagaaaaaa gaatttcttc
    25801 ctaagacagc agttacaaat aggaaggaga ataaaaaaga acgccagctc tttgcaaaag
    25861 gtgatcggat atttgtcaat gaataccaaa aagaagcgtt agtttatgaa gatataggtg
    25921 aagatacgat tgatgtatat ttagacaaaa aaataatcca cgtgcctcgt cagcgtgttc
    25981 gtctggtgcg ttcagcagaa gatctgtacc cgacaggtta cgatttagac agtctgttta
    26041 tcgattataa aacacgtaaa cggcagcgtg acctagagcg aggatcaaaa aaagctcata
    26101 aagtactagt aaaagaaatg cgcaaacgac aggaagaaag aagagtaaac gatgaaaaca
    26161 gcaagtgatt atctcaaatt agctcataat atcagtggct gtaaagaaaa ataaaaacag
    26221 tgaaatcatt ggttttattc cccgtgtaag ctaaaataaa aagaaaagag tgctgaggag
    26281 gtagggcgat aaaatgaaat taagcacaga ctggcgtaaa gaaatacaaa cgattccgaa
    26341 tcttttatcg atattccgta tttttttgct gccgatttat ctttattttg tgctgagaca
    26401 gtcattttat atagcaggag cggtcattgt tgtctcaggt ttgtctgatt atctagacgg
    26461 ggtcattgct cggagatata atcaggtaac agacttaggg aaggtgcttg atccgtttgc
    26521 agataaattg acacaacttt ttctgatttt gtctatggca tggtatcgac cgtggctttg
    26581 gctgttgttt ggtctattct tgataaaaga aggctttatg tttgtagctg gattaatcgg
    26641 gctatctaaa aatatcaaat taagtggagc gaagtggtat ggcaaagtag cgactgctgt
    26701 catctatgtt ggcatgatcc ttttgctgct tttcccagaa ttaccaacgc tgtgggtgag
    26761 agtcattttt gccgtcatca cttacggtct tttacaatct tttgtcttgt atgctgtcga
    26821 gtaccgaaag atgtttcaaa gaaaataaaa aaggggtgtg acctatgtcg cattcctttt
    26881 tttgataaat agactcccag tagctacttc tcgataataa gccaaccaaa ccaaaaacag
    26941 gagaagttgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg ctcacaacct
    27001 cttggtaaac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    27061 gagcagctat ttttccgttt gtttctctaa tactcaggtc aaaccgctga ttttacaacc
    27121 tcttccttaa acggtattca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
    27181 tgtagaaatc cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
    27241 atctcttcct taaacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    27301 aatatgagga gctattttcc cgtttttttc tctaatactt aggtcaaacc gctgatttca
    27361 caacctcttc cttaaacgg
//
LOCUS       DACCPY010000032        27083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCPY010000032 DACCPY010000000
VERSION     DACCPY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.032"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IVO69_001181"
     CDS             7..624
                     /locus_tag="IVO69_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399971.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IVO69_001182"
     CDS             1644..2813
                     /locus_tag="IVO69_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7399972.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IVO69_001183"
     CDS             3286..3840
                     /locus_tag="IVO69_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7399973.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IVO69_001184"
     CDS             complement(4013..4486)
                     /locus_tag="IVO69_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7399974.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IVO69_001185"
     CDS             4498..5034
                     /locus_tag="IVO69_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7399975.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IVO69_001186"
     CDS             5074..5445
                     /locus_tag="IVO69_001186"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7399976.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IVO69_001187"
     CDS             5729..7000
                     /locus_tag="IVO69_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7399977.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IVO69_001188"
     CDS             7245..8270
                     /locus_tag="IVO69_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7399978.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IVO69_001189"
     CDS             8564..9673
                     /locus_tag="IVO69_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7399979.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IVO69_001190"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IVO69_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IVO69_001191"
     CDS             10668..11492
                     /locus_tag="IVO69_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7399980.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IVO69_001192"
     CDS             11524..12834
                     /locus_tag="IVO69_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7399981.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IVO69_001193"
     CDS             12982..14241
                     /locus_tag="IVO69_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7399982.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IVO69_001194"
     CDS             14251..15123
                     /locus_tag="IVO69_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7399983.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IVO69_001195"
     CDS             15135..15965
                     /locus_tag="IVO69_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7399984.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IVO69_001196"
     CDS             16005..18188
                     /locus_tag="IVO69_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7399985.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IVO69_001197"
     CDS             18249..19469
                     /locus_tag="IVO69_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7399986.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IVO69_001198"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IVO69_001198"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7399987.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IVO69_001199"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IVO69_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IVO69_001200"
     CDS             22431..22640
                     /locus_tag="IVO69_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7399988.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IVO69_001201"
     CDS             22633..22782
                     /locus_tag="IVO69_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7399989.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IVO69_001202"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IVO69_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IVO69_001203"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IVO69_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7399990.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IVO69_001204"
     CDS             25304..25537
                     /locus_tag="IVO69_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7399991.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IVO69_001205"
     CDS             25534..25941
                     /locus_tag="IVO69_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7399992.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26392..26901)
                     /locus_tag="IVO69_001206"
     CDS             complement(26392..26901)
                     /locus_tag="IVO69_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399993.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         9318 a         4024 c         5037 g         8704 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatact tggatatgct ggaatgttta agagaataat tacatttcta
    26101 gaaaatacaa taaaaattac aggataatta gccatatcct acctcctcta gcagttgctc
    26161 aaaagttagc actaagtaac ttcttttttg ttgctggtaa caatttatca atactaaaat
    26221 agcgtttaag tatctaataa atactaattt taaagctctt aattattatg aaacacgata
    26281 taacaatatt gctatatcgt gctattgtaa tattatacat ttgaagacag cgtattcttg
    26341 ttctaatatc aggaattttt tataaatatc agtcatatta agactcaaaa tttattggta
    26401 tgttcctctg tatagagttt gaccgtctac cattatatag caattattat cattgttgtc
    26461 ttgcaccaat ataccgttaa gggtagcact aggagtagta acaatcactg gaaactcatg
    26521 agaaagctcg gtcatttttc ctgttctgct agaatcgtgt tctattgtca agccatttac
    26581 tataaacgtg aatactttac ttcctattgt tatagtagct ttttgagaaa cagtttctgt
    26641 agctggagct gtgcttcttt ccgaatttgt tagggtttca ctggattcat tgctataaac
    26701 aattgtagag gattctacgg cttcttgagt gtccacgttg gtgctgctag cttcatctag
    26761 cttccttttg tattgttcgt tactgattgc taagctttcc cgttggtcat caagcttatt
    26821 catttctgta atggtataaa ataatcctcc tgctagtgct atattaacaa ctaaactaac
    26881 cgcaaataat acctttttca ttcttccttc tcttctttct ctttttgtaa ttccttactt
    26941 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
    27001 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
    27061 acaaatcgtc aatctttact tag
//
LOCUS       DACCPY010000033        26353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCPY010000033 DACCPY010000000
VERSION     DACCPY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.033"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVO69_001207"
     CDS             complement(99..1229)
                     /locus_tag="IVO69_001207"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7399994.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVO69_001208"
     CDS             complement(1878..3080)
                     /locus_tag="IVO69_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7399995.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVO69_001209"
     CDS             complement(3140..3343)
                     /locus_tag="IVO69_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7399996.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVO69_001210"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVO69_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVO69_001211"
     CDS             4880..5692
                     /locus_tag="IVO69_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7399997.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IVO69_001212"
     CDS             complement(6716..6868)
                     /locus_tag="IVO69_001212"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7399998.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVO69_001213"
     CDS             complement(7059..7937)
                     /locus_tag="IVO69_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7399999.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVO69_001214"
     CDS             complement(7976..9094)
                     /locus_tag="IVO69_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7400000.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVO69_001215"
     CDS             complement(9091..11190)
                     /locus_tag="IVO69_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7400001.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVO69_001216"
     CDS             complement(11241..12062)
                     /locus_tag="IVO69_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7400002.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVO69_001217"
     CDS             complement(12059..12868)
                     /locus_tag="IVO69_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400003.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVO69_001218"
     CDS             complement(12889..13383)
                     /locus_tag="IVO69_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400004.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVO69_001219"
     CDS             complement(13395..13883)
                     /locus_tag="IVO69_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400005.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVO69_001220"
     CDS             complement(13960..16656)
                     /locus_tag="IVO69_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400006.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVO69_001221"
     CDS             complement(17084..18157)
                     /locus_tag="IVO69_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7400007.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVO69_001222"
     CDS             complement(18154..18981)
                     /locus_tag="IVO69_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400008.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVO69_001223"
     CDS             complement(18982..19788)
                     /locus_tag="IVO69_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400009.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVO69_001224"
     CDS             complement(19800..20885)
                     /locus_tag="IVO69_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400010.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVO69_001225"
     CDS             complement(20902..21444)
                     /locus_tag="IVO69_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7400011.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVO69_001226"
     CDS             21702..22691
                     /locus_tag="IVO69_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7400012.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVO69_001227"
     CDS             22688..23425
                     /locus_tag="IVO69_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7400013.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVO69_001228"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVO69_001228"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7400014.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVO69_001229"
     CDS             23989..24621
                     /locus_tag="IVO69_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7400015.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVO69_001230"
     CDS             24706..25353
                     /locus_tag="IVO69_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400016.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVO69_001231"
     CDS             25358..26341
                     /locus_tag="IVO69_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7400017.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8061 a         5570 c         4465 g         8257 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gat
//
LOCUS       DACCPY010000034        26031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCPY010000034 DACCPY010000000
VERSION     DACCPY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.034"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..594
                     /locus_tag="IVO69_001232"
     CDS             <1..594
                     /locus_tag="IVO69_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7400018.1"
                     /translation="FFKRMIDIVGSIIGLILASPLMFYVAYRIRKEEPGSPIFFCHTR
                     VGKNGKTFKMYKFRSMCLDAEEKLEELLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSI
                     DELPQLWNVLKGDMSLIGPRPPLEREVAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSF
                     HKMVELDLEYISHRSLMYDLRLILKTFYVIVKPNGAY"
     gene            1821..2501
                     /locus_tag="IVO69_001233"
     CDS             1821..2501
                     /locus_tag="IVO69_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7400019.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            2530..3843
                     /locus_tag="IVO69_001234"
     CDS             2530..3843
                     /locus_tag="IVO69_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7400020.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            4649..5416
                     /locus_tag="IVO69_001235"
     CDS             4649..5416
                     /locus_tag="IVO69_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP7400021.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            5443..6444
                     /locus_tag="IVO69_001236"
     CDS             5443..6444
                     /locus_tag="IVO69_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP7400022.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            6467..7753
                     /locus_tag="IVO69_001237"
     CDS             6467..7753
                     /locus_tag="IVO69_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAP7400023.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            7787..8440
                     /locus_tag="IVO69_001238"
     CDS             7787..8440
                     /locus_tag="IVO69_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7400024.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            8473..9678
                     /locus_tag="IVO69_001239"
     CDS             8473..9678
                     /locus_tag="IVO69_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400025.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            9808..11133
                     /locus_tag="IVO69_001240"
     CDS             9808..11133
                     /locus_tag="IVO69_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400026.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            11150..12280
                     /locus_tag="IVO69_001241"
     CDS             11150..12280
                     /locus_tag="IVO69_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400027.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            12306..13391
                     /locus_tag="IVO69_001242"
     CDS             12306..13391
                     /locus_tag="IVO69_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAP7400028.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            13414..14229
                     /locus_tag="IVO69_001243"
     CDS             13414..14229
                     /locus_tag="IVO69_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400029.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            14213..15931
                     /locus_tag="IVO69_001244"
     CDS             14213..15931
                     /locus_tag="IVO69_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP7400030.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            15957..16793
                     /locus_tag="IVO69_001245"
     CDS             15957..16793
                     /locus_tag="IVO69_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400031.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            16798..18231
                     /locus_tag="IVO69_001246"
     CDS             16798..18231
                     /locus_tag="IVO69_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7400032.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            18248..19909
                     /locus_tag="IVO69_001247"
     CDS             18248..19909
                     /locus_tag="IVO69_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7400033.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKR
                     YSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALL
                     SVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELA
                     KLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDL
                     KKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            19997..20395
                     /locus_tag="IVO69_001248"
     CDS             19997..20395
                     /locus_tag="IVO69_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7400034.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            20420..21136
                     /locus_tag="IVO69_001249"
     CDS             20420..21136
                     /locus_tag="IVO69_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400035.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            22368..22947
                     /locus_tag="IVO69_001250"
                     /pseudo
     CDS             22368..22947
                     /locus_tag="IVO69_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            23007..24659
                     /locus_tag="IVO69_001251"
     CDS             23007..24659
                     /locus_tag="IVO69_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7400036.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            25003..25368
                     /locus_tag="IVO69_001252"
     CDS             25003..25368
                     /locus_tag="IVO69_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP7400037.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            25456..25899
                     /locus_tag="IVO69_001253"
     CDS             25456..25899
                     /locus_tag="IVO69_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400038.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT         8344 a         3642 c         5632 g         8413 t
ORIGIN      
        1 ttttttaaaa gaatgattga tattgtagga agcattattg gtttgatttt agcttctcct
       61 ttgatgttct atgtagctta tcgaatcaga aaagaagagc caggaagtcc gatatttttt
      121 tgccatacac gtgttggtaa aaatggaaaa acattcaaaa tgtataaatt ccgttcgatg
      181 tgtttagatg ctgaagaaaa attggaagaa ttacttgatc aaaatgaaat agaaggtgca
      241 atgttcaaga tgaaagaaga tccccgtgtc actaaaatag gtaaaattat ccgtgccaaa
      301 agcattgatg aattgccaca gctatggaat gttttaaaag gcgatatgag tctaattggt
      361 ccacggccac ctttggagag ggaagtagct gagtacacga gatatgatct tcaacgcttg
      421 tatgtaaaac ctggttgttc aggtttgtgg caagttagtg atagaaataa tgttagtttc
      481 cataaaatgg ttgagttgga tttagagtat attagtcatc gaagtttaat gtacgacttg
      541 aggttgattt taaaaacgtt ttatgttatt gtcaagccca atggagcata ttagttaggg
      601 atagaatgat ggattgttaa taaaaaagat tacacatgtt catattatcg tttcaaaagt
      661 aataactgta attatggagg gtttgaatca cttgtagaaa atctaaaaaa agaaaggtga
      721 atggggcaat tcagtatcat tattcttata taaaagaaaa tattcgaaat tttgggatga
      781 taccactctt aaatataatg atctaaatgc tttaatattg gtatatccga tataggaact
      841 gacgagctat ttagtatgat acaaaagtat attgatagcc attaataaat tttccaaaaa
      901 ataatgacag cacaccaatt tttataattt agcttgttga atcggtatgt tcatgggacg
      961 gttcaaaaaa agaatcgaaa aaataaacgg taaaatttta gtgaatccta tgtaataaaa
     1021 tttggataga tggaaaaaca gcacataagg aggctgagtt tgttgtcatt tctgctccta
     1081 caaactatgg tagtgaatgt gttattgaat tagtgatgtc tgttacttca gagacattgg
     1141 ggataattaa cttcactata cctgtagggt atacaaaaaa tggaagaaaa aatattatca
     1201 atagatttta tttagttcag aatttttacg tggaagtaaa ttattgatga taatctttat
     1261 ccaattagaa ttattatcgt aggatgtgat gatgatagcc gtgaagttgt tcaagtattt
     1321 accaatgtct acaacgcaga aaattagttt gggtttttcg gattggaaaa tttcaatgta
     1381 taaaatcata aatttgctgt aaatagacag tttggggtag taatagccca ataaattgtt
     1441 cgataaactc atctttgggg tgagaatagc tcaatcattg aatttcaaac ctgcctttgg
     1501 ggtgggatta gaggtcaata tgtacctaca taatactttt tggaaaatga tggtgttgag
     1561 aatgtaagta catttcaatg tgaaattcta atatagcccc aaacgaggtc aataaaaatg
     1621 acctaaactc gtatttctcc cctaaacgag gaggagatag atatgaaaat tgaatgtgcc
     1681 ggcgttggag ttggctgctt ttagtaggaa tgaacatggt tcgatgagaa ctgtttcaga
     1741 tagagtggtc gatgatcaca ggcctttaag tcgtttacag atgatgagac ttgtaaaaat
     1801 agagggacga atggaaaaag atgattggta tagaagaaaa gaaaggtgtt cagaaagacg
     1861 taacaattga tagggaagag aatcttaggg gacatagatt ctattggatt ggacgcagag
     1921 cacaagatat cttcttctcg gctctagctt tgattgcatt gagccctgtg atgctaatta
     1981 cagcgctcgc cattgtgatt gacgatcccg gtggcagccc gatttttgtg caggatagag
     2041 ttggaagaaa tggaaagctg ttcaagtttt ataaattccg atctatgtgt gtagatgctg
     2101 aagcaaaatt agaagccttg ttggcacaaa acgagatgga tggtccggtc ttcaagatta
     2161 aagatgaccc aagaatcacg agagttggta aatttattcg aaagacaagt atcgatgagt
     2221 tacctcagct ctggaacatt cttaaaggtg atatgagtat tattggaccc agacctgctt
     2281 taccgagaga ggtggaacaa tacggtgact acgaaaggca gcggttgttc gtacagcctg
     2341 gtcttagttg ctattggcag attgctccac atcgtaatga actgtccttt gaagaatggg
     2401 tggaacttga tgtgaagtac atcaatgaaa gaagctttat cacggactgg aagattatct
     2461 tcttgacttt acaggctgta ttatttggat atggtgaatg attatatata gtattaggag
     2521 ttgaaatatg tgaatattat tttattatcg ggtggtagcg gtaggcggtt atggccgctg
     2581 tccaacgata ttcgtagcaa acaattcatc aaactgttta aggatgaaaa tggcgaatat
     2641 gaatctatga ttcaaagagt ttatcgccag attacaactg ttgacccaga tgcaaaggtc
     2701 actattgcaa caagtaagtc tcaggctagt gcaatccata atcagttggg agataaggct
     2761 tcgatttgtg tagaaccttg tcgtagagat actttccctg caattttgtt agctgcagca
     2821 tatcttcacg atgaacttgg cgtaggtgat gatgaaagtg ttgcggtttg tcctgttgat
     2881 ccttatgtag ataattccta ttacgaaggc gttgaagccc ttgagaagtt ggtgcagcag
     2941 aatacggcta atttgacttt gatgggtatt gcacctacat atcctagtga aaagtacggt
     3001 tatatcatcc ctgaaagttc taacgaagta agtactgtta aggagtttaa ggaaaaacct
     3061 gacaaagcaa cggcagagaa atatttagct cagaatgctg tttggaatgc cggtgtattt
     3121 gcgttcaaac ttggttacct gttggaaaaa gctcacgcaa tggtggattt tgaagattat
     3181 cgtgatttgt attccaagta tgagacttta accaagatca gctttgacta tgctgtggtc
     3241 gaaaaagaaa cgagcattca agtgatgcgt tataacgggg attggaaaga tgtcggaact
     3301 tggaacatga tggctgaagt tatggctgat aatattaagg ggaaagctgt aatggatgat
     3361 acctgtgtta atactcagat tgtgaatgag ttgaatattc ctattcttgc tatgggctgc
     3421 aaagatatgg taattgcagc aagtggcgat ggaattctga tttctgataa agagcgcagt
     3481 ggctatatga agccgtatgt tgagaaaatc agtacggaag taatgtttgc agaaaaatcc
     3541 tggggtacat ataccgttat ggacgttcag gaaggctcca tgactgttaa agtttctatg
     3601 caggccggac agaagttgag ctaccactac cacaattctc gcgatgaagt ttggactgtt
     3661 gtagcaggac agggtaaagc gattgtagat ggtatgaagc agattcttcg tgcgggtgat
     3721 gtggtgacta ttgccgcagg atgtaaacat acaatcgaag ccgtgacaaa tctggatatt
     3781 atcgaagttc agattggtga aactattagc gtcgctgaca agtataagag ttctttgaac
     3841 taaaagttcg cgaggtagtt attatggaga tactaaaatt tcaacccgtg ggtaaagatt
     3901 atctttgggg tggagaacgg tttaagacgg aatttggaaa aaattgactt aacaccatta
     3961 agcttatacg tgggaatgct tagttcatct agacgataac tacgtacgga aatatcaaat
     4021 atgaaggaca gaatggtaaa accgagttgt ggtatgtatt ggaagctaca ccggggggtg
     4081 atttatgctt ttgagtacga tgtaacagaa gaaatgttac gaaatgctgt gatggacaga
     4141 tcacttggga aacatttata taaagaagaa gtacacaagg gagatctttt ttatgtcagt
     4201 tcatggtatt ggcgctggag tattagtggc tgaaatacag gaaagctcaa atgttaccta
     4261 tcgagtttac gattatgatc gagtagataa gaatgataat aagtgagaat tgtactttga
     4321 aaaagcgatt caggtaatga atatggaagc cactttgaaa ttggagaaaa gaaataagct
     4381 cattcatttt tatcctggtg gctctcgtga aatactgtgt agatgtaaat actgtgagcc
     4441 cgaaagaatc caagtaaaga cggggttttc gttctcggta aaagaggaat cttttcaagt
     4501 tcttttatgt atatcggggg acggacaagt tgagtcggca aatggtgtta tgaaactcat
     4561 tagatttgta aaaaagatat tgtatgtttt tgccaacgaa tattggaaga tgttaagtgt
     4621 tgagcgatgc ggacttactg aaaattagat ggggatagag ggaggatatc taatgtcacg
     4681 aatgagattt atgaacacag aaattgacaa tcttacaatg ggtgaagtat tggtagaggt
     4741 tgaaaaaatg attcaagata ggagatgcgg atatgttgta actccgaatg tggatcacat
     4801 tgttcagttg gaaacaggaa aagagttgca agatgtttat aaaaacgcat ctttaatttt
     4861 gacggatggt aaaccattga tttggatttc aaagttttat aagacaccaa taaaagaaaa
     4921 aatatcggga tccgatatat tttctttact gtgcaagatg gctgcagaaa agggatacac
     4981 aatgtatttt ttaggtgcgg cagagggcgt tgcggctaaa gcggcagata acctgatgaa
     5041 aagatttccg ggattaaatg ttgtaggaac atactctcct ccttatgggt tcgaaaaaga
     5101 cgaagatgaa atgtttaaaa ttgattccca aattaaacga gcaaatccag atatgcttat
     5161 tgtgggtttg ggttgcccta aacaggaaaa atttatgttt tataactgtg atagattggg
     5221 agtacctgtg tcttttggtc taggagctag tttggacttt gaggcaggga atgttaaaag
     5281 agcaccaaaa tggatgtcag attatggatt tgagtggctg tttagaatta ctcaggaccc
     5341 taaaagaatg gcaaaacgat atttggttga tgatttaaaa atctttaaat tagcactaaa
     5401 atatcggaaa aaataaacta tattcaatga ggacaatgaa gtatgattat tacaaaaaca
     5461 ccttttcgca tgtctttttt tggcggcgga acggatatgg aagattattt tcgagaaaat
     5521 ggcggtgcag tgctttccac tacttttgat aagtactgtt atgtgaatgt gcgtcatttg
     5581 cctagatttt ttgattacag cacagagtta tcatattctc gtactgagag agtaacaaat
     5641 attgaagata tcgagcatcc tgcaattcgt aatgcgatga aaatgctaga tatgcatgaa
     5701 attcgcctta catatgaagc agatcttcct gcacgttctg gtctgggtac atctagttct
     5761 tttgccgtag gtatgttgaa tgctttctat gctttgaagg gcaagtacgc tgacaagaag
     5821 aaactcgttg ataaagcaat ttatcttgaa agagtccttt gtaatgaagc aggcggatgg
     5881 caagatcaga ttgcagcagc ttatggtggc tttaaccgaa tcaatttcaa ctcagatggc
     5941 tatgaagtat tgcctattat tattacgcct gagcgtaaaa agcagcttaa caacaacttg
     6001 atgatgttct ttactggatt caccagattt tcctctgatg ttcagaaggc aaataatgtc
     6061 agcggcacag aggacaaaag agtaagactg aaaaaaatgt atgagcttgt ggatgaagca
     6121 gaagcggttc tgactgataa gaacagagat ctagatgatt tcggcagact gcttgatctc
     6181 acttggagat tgaaaaaggc taccggtggt gcgattagca ctggaagtat cgatgaactt
     6241 tatgaaaagg gcatggctgc tggtgcattg ggcggtaaac ttctcggagc aggtggcggc
     6301 ggtttcttgg ttttttatgt tcagcctgaa aaacaaaatg ctgtgcgttc ggcaatgagt
     6361 aatttgatgt atattccgtt tgaatttgaa aacggtggaa cacaggttat tcattacaca
     6421 ccggaaatgt atattccaaa ataaagaaat cagcgaggat aagaatatga aagttgttat
     6481 tatggcaggc ggtaagggaa ctcgcatctc atcggttgca agcgatattc caaagcccat
     6541 gattaaaatt gaaaacaaac cggtattgga gcacgaaatc gaatgtctga gagaccaggg
     6601 attcacagat attattctta ccgttagcca tcttggcgat atcattatga actattttgg
     6661 cgatggttct ggaatttcac cagttactgg tcagcccttt gacgttaaaa ttgaatactt
     6721 tttggagaaa gagccattag gaaatgccgg tgcattgttt cgtttaaagg ataatttgac
     6781 cgaagacttc ctcctgctga atgcagatgc gatgtttgac gttgatttca accgatttgt
     6841 agcatatcac cgtgaaaagg gtggcttggt gacattgttt actcacccta acagtcatcc
     6901 atatgacagc ggtctaatca ttgcagataa ggatggctct gtagacagat ggcttgtaaa
     6961 ggaagattcc cgtccaactt attaccgtaa cagagtaaat gcgggactcc atgtgattag
     7021 tcctgctgtt cttgatgtcg agataaacac acctaagatt gacttggacc gtcagttatt
     7081 aaagccgttg gcaggaaccg gaaaaatgtt ctgctatgat agtccagaat acgtaaaaga
     7141 catgggtacc cctgatcgtt attatgctgt ctgtgaagat tttaaggctg gaagagtgaa
     7201 gggcaaaaat cttcagaata agcagaaagc tgttttcctt gatcgtgatg gaacgattaa
     7261 tcaatatgtt ggctttctgc gtcatattga tgagttcgaa ctaatcaagg gtactgaaga
     7321 tgcagtgaaa aagatcaatg ccgctggata tctggctatt gtaataacga atcagccagt
     7381 aattgcaaga ggtgaagttt cttttgaaga attggaagag atccataaca aaatggagac
     7441 acttcttgga tataagggcg cttatttgga tgcagtttat tattgcccgc atcatccaca
     7501 taagggatat gaggaagagc gcccagagct aaaattcgat tgtgaatgca gaaagccaaa
     7561 gcccggaatg ttaattcagg cagctacgga cttcaacata gatttgagtc agtcttggat
     7621 gattggcgat ggcgagaatg atattaaagc gggccaagcc gccggctgta agactgcatt
     7681 ggttggcgag agtgagtatg gacaggatat gaccatagca accctgagtg aatttgcaga
     7741 taagatctgc taagggatgt tttatcacga aaggagagta taaacaatga tagacaatag
     7801 attacagaaa cacattgatt tgctgattaa acgctatccc actcttgaag taatcaagca
     7861 ggatattgtt gacggatacc tgattatgga ggaatgctat ttaaatgatg gcaagctgct
     7921 gatagcagga aatggcggat ctgctgctga ttccgagcat attgcgggtg agctgatgaa
     7981 aagctttaag acgcctcgcc ctgttagtag ggaatttgca gataaattga ttgcggttga
     8041 tcctgtaaag ggttctgaat tagctaagaa tcttgaatat ggtttgatgg cgattcctct
     8101 cgttgctcat gaagcgcttt ccactgcgta tattaatgat gttgatggcc taggtgtttt
     8161 cgcacagcag ttgtttggat ttggaagacc tggcgatgta ttccttggta tttcgacatc
     8221 cggcaatagc aaaaatgtga taaatgcaac tgttgttgct agagctcttg gtatgaaagt
     8281 aattggctta actggagcca aaggtggaga acttgcaagg gtagcagatg tttgtgttaa
     8341 agctcctgaa acagaaacat atatgattca ggagtaccat ttgccaattt accactgttg
     8401 gtgtctaatg ttggaagata aattttttgg gaatagataa aatatctaag cccatattga
     8461 aaggataaaa ttatgaatgt actatttata acaactcaat ttccatatcc tctagataat
     8521 ggcggaaaaa ttggtgcgta caacggtatc agtgttttat ctagagacta cgatgtgacg
     8581 gtattgtctt tcaccgaaga accgcagtat gttgaagaag gtgtcagttt ttttcgagag
     8641 aaatttaata gtgtaaaatt tgaaaatcct gtgatacatg atgtacacat aagaaataat
     8701 ccaatgaaat tggtagaagc tttagcaata gggtattgtc tacgccaacc ttatatagtt
     8761 tcaaagtttt atgattcaaa aatgctgcag ttaatagatg aaaaattttc taacaatgca
     8821 tattgggata tcatttttat tgactatctg aatatgcaaa gttatggtca atacatacgg
     8881 gagaagtata aaacacaatt tggtaagtat atcttaaaag accacaacat agaatatgat
     8941 ttagtcctac aggaagctga atcaagtcga ggtttaaaaa agaagttgct tcagttagag
     9001 tataagcgta ctcgaaagta tgaagaggcc gcagtaaata gggccgatta ttgctttagt
     9061 gtgtgtgatg caaatacgga agtactgaaa aagattaatg acaacagtta tacgatgctt
     9121 ccaacttatg aaatgcttac aggggtgaaa aaaaatatag agaagaacac gatcctatat
     9181 ttgggtaacc tttcttggaa atccaatatg caaggattag tttggttttt agagtcaatt
     9241 tatccaaata ttcaacaaag aattccggat gtaaaaatca caattgtcgg tagcgggcca
     9301 gttggaacag attttagtaa atattcaggc gtggattatc gtgggtatgt aaaagatatt
     9361 tcacacattt atgacgatca agcggtgcta attgtacctt tgttagaagg tagtggtata
     9421 agaataaaaa tattagaagc gttcaataat gaaattgcag ttgtatctac attagttggg
     9481 tgtgatacga ttggcgcaaa agatggaagt gagttattta tagcagataa cgaaaaagat
     9541 tttgcaacgg ctgtagttag actgcttgaa gacatgaatt tgaatgaaaa agtaagaaag
     9601 aatgcaaaat tattcttaaa agaaaaatac tccttaacgg ctagacaaga tgagtttagt
     9661 gcgataatta acagctaata tatatataag cttatctgat gatgatttat aggaggaaat
     9721 atatggagat ggctgagaga aaggaaaaga aacttggaaa taagaatatc atattatttt
     9781 agaaaactcc tgttatttta tgtttgtgtg attgttgcgg ctatgagtta ccaccttaca
     9841 ggcaattcca atgtagcgtc attaattgct gtagtaggag tagtttatgc tattgtaaag
     9901 gcgtgtaata acgagtatct ggctatatgg gttatgtttg tattaggttc tgatatatgg
     9961 gggatagtgc catatattaa cattggtagt ggtgtgttgc attggtcaga ttttaatgtt
    10021 atagtagcga tatttctctg cttgagattc atcaaagtta aagctaggag tagatacggg
    10081 attgtctata gtgtaatgct tatatttatt ttgattgcat gtgtgcaatc gcattcaatc
    10141 tatggacaag atgtacatac aacgctctta acagctagag aattgctagt tgtaatatcc
    10201 ttttggccga tagtaaaaat agtatattca gaaagaataa ctaaaaatga attcattgat
    10261 ttagtgtgta aattcacaag atggacaatg tgtatatatt ggtttgaatg gattcttgtt
    10321 aatatgggat tcaatataac ctttttacag acaggtgcac gctggggtac tagaatctat
    10381 atagatccga tatttatcat tttatatttc tttataaatc tatatgcggt tatatataaa
    10441 agagagggct ataatagtac aaataaaagt atatggaatt gcatttttag tttactgaca
    10501 tttgttataa tctcgcaagg cagaagtggt atattatatg tagtaataac ctcggttatt
    10561 atgcttttaa tttcgcttaa accatccaag attattagat ttggtatatt aggaattgcc
    10621 ttaattgcat gtctagtgtc tgtgacccaa attagagagg tcgttatatc ttcgtttgta
    10681 gagtctcaga gcagtgacac aggtaatatc gcatatagaa ttgctgaaca agattatttt
    10741 gaaaataaat tagaaggtca tgaactattt ggagttggta taccaaacaa tcatgatgcc
    10801 agagctgtag aatattcagg caaaacaata gattttgagt ctaaaaaata taatccctat
    10861 tatcttgagg acttaggcgc ttttgaaatt cgttatagat ttggtataat tgcatatgcg
    10921 ttatatatag ttgctttagc tataagtatt atagatgccg gatttcgaag agacaaatcg
    10981 gtcgccgcat ttgcgggtat ggcattcatg gtgtttatgg cgcttaattt taatttgcta
    11041 gcgtatatta cagcaagacc gttcggattt atgatttctt taattttgat agaaatatcg
    11101 gcattcaatg atgaattcat atatattgaa taggtaaggg agagcacaga tgaatttaga
    11161 tcgattaaag aaaatagcaa atatgatttc accaatcata aaagtgataa atgcgccgtt
    11221 agcgtatttt agtgggaaat cagattatat ggagaaggga atattgtggc ctactgtaac
    11281 aacctactcc aataacagta gaaaagtgat tcaatataca actttaccta aaaacgtaga
    11341 caacatggct caaatgcaat gggctgggga aagttttgag gacatatgta tagttcttca
    11401 aggtccagta ttagaagacg gtgattttac actaaatacc gttaaaatgt ataataaata
    11461 ctatcctaaa gtaaaacttg tagtgtctac ttggatagga ttatctgaag agacagaatg
    11521 caagttgcgt gaagctggtg cagatgttgt attatgcaag aaacctgaaa aaacaggtgc
    11581 gtacaatatt aatctacaac ttacttctgc atacgaaggc gtaaaaatgg cgcaaaaact
    11641 ggggtgtagt tatgcgctga aaacgcgaac tgatcaaaga ctatatgcaa atgatgtact
    11701 aaagtatttc caaacattat tgaatatttt tccatcttcg gactgtaaag tagtgaatca
    11761 gcgattaata tttacgactt atggaaaatc atttagatat cttccgttta atttatgtga
    11821 ttttttggta tacggagaaa ctagtgaatt attaaaatta tatggtattc aaagagataa
    11881 ccgagcatca gactttggga aaattattta tactgaggaa aaagagttta ataaaaagat
    11941 atttacacta ctagaaggaa aacaaggtgt atctccatat gatgtttttc cagattttga
    12001 tgcactatat aaaaaatata tgtttgcaga atactatatt gtgtataatt atttcctgga
    12061 aaatattgca agtaatgaat cttatggtga cttaatggat gcctattata gttatttaaa
    12121 gaaatatgca gttgttgctg attcagaaaa attgttacta tattggccga aatatgttca
    12181 atataatgtt aagttcgaaa atgaacttga tttctatgcg aagttggatt caaaaaaatg
    12241 gatggatatt tatgctaatt atacaccttt tagtcagtga ttatatggaa aggatgaggt
    12301 gaataatgaa aatcggaata gtgactattg ttaatggggc aagttttggt aacagattac
    12361 aaaattatgc gactcaaaaa gtgttggaaa ataacggcta tacagttgag acaattaata
    12421 attgttttga caaagaaagt accctatcca ttgttatcaa aaacgctatt tatacattat
    12481 ttaagtttat accgcatttc aaaaagtcaa aaagtatctg gaaaagaatg tatacatttt
    12541 atcaatggga caagcagtat atagattatt ctcatcgtaa aattgatgca tctaaaaaaa
    12601 acagctttgt gagtataaag aatgattatg actatttctt ggttggtagc gatcaaattt
    12661 ggaatccaaa atatgcagcc aataagggat ttgaactttt atcatttgtt ccaaatgaaa
    12721 aaagagtttc attatcaacc agtttgggag ttccatctat accaagtgat ttagaaaaat
    12781 tatatgtgac agagtggaat aagtttaaat caatatcggt acgtgaaaat caagcggcaa
    12841 aaataattga ggacttgaca ggcacaactg cggctgttca cattgaccca actttaatgt
    12901 tggagcgttc ggaatgggaa gcctttcaaa aaaaaccaga caatattgat gaaaattaca
    12961 tcttgtcgtt tttcttaact aatccgccta aggatactgt tgatgtatta gataaggtcg
    13021 ctaaagattt gaaaataggt gttctcacct tagatgcgtc ttgggaatat tttcaagata
    13081 ttactccgaa tcattttgtt tatttgattt cacatgcaag aatgatttat acagattcat
    13141 tccactgcac agttttttca ttgctatttg gaaaaccggt tgtggtattt gaccgttttg
    13201 atagtgaaaa aatgaattct aggttaagta cattgttaaa taaagtaaat aaagcacatt
    13261 tttgggtagg aaaaaaagct gatgaagcaa tgattcgtgc aatagcatct gaacctgaat
    13321 atgacataga taagctgatg gtatcagaga gggaatgtgt aaaagcttat cttaaagagt
    13381 atttacagta aaattttgaa atgggatgaa aatatggagc atttaagtga ttgttttggt
    13441 aaaatgaaga ttgttacgac ttcatgcgca agagaatatt ttaaggatga tttggataaa
    13501 tcagagtatt catgttttgt tgattctcga gcagctatgt ttttcgcaat gggacaagct
    13561 ttagctaaaa gaaggaatgt agttgtaatg cttgctgatg aattgacatc gtgttatacg
    13621 gcactaacag aatgtgcatt tcaacaagtt gatttaattg tgtttgcttt tcagacagtc
    13681 agacatgaaa ttgatttagc atctttgaag aattgtggtt gcaatctaat taactgtagc
    13741 gcccaagacg atattgatag agttgtgtcc aacactaagg ggttaaatca gacaacagta
    13801 attaaaatca atatgaattg tgagtcggat aaagatcata tatgtgagta tatagacctg
    13861 ccagacatac ctgttttttt ccacggtagc ttatactcac ctgcaagtaa aaagaaatat
    13921 gagcagtatg gacaggtatc gaagtattta ggctatctcg tggggggaaa agatgcaata
    13981 ctcataattt ctcacaatga gtttttaaac gatatcagtg tatggattaa ttattattct
    14041 tttggagaaa ttcaacccaa aattattata tttgacagtc cgaatgacta tcaatataaa
    14101 tggctgtgtg acttagaatg cagtgtagct cattcaacca accgattttt gtctgaggat
    14161 ttagccttat tgcaaaataa cggaatttcc attgtgttaa gaggtgataa ttatgatatc
    14221 gtcagttaaa aatgttcaaa ttttagtatc tttattaaaa cagcataata ttaaagatat
    14281 agttatctcg ccaggtagta gagattttca attggtgcat agtgtagaaa cagacagttt
    14341 ttttagaacc tatacggttg tagatgagcg cagtgctgcc ttttttgcta atggactttc
    14401 tatggcgatt gataagcccg tgtgtgtatg ttgtacttct tcaactgcct cttcaaatta
    14461 tatggcggca gctaaagatg cttttgctaa tggcatccaa cttgtacttc ttactgctga
    14521 tagagattac cgccgcttat atcagatgga agatcagatg attgatcagg tagatatgta
    14581 tggggcttat gtgcgttcta acgttgatat tcctattgtt aaggattctg atgatgaatg
    14641 gtatgctacc agaaaaataa atgaagcaat attggagtta aatcatggcg ttaagggtcc
    14701 cgtgcagatt aattatcaat taatggattt aagtgcgccg tttgttgaga ccatgccaga
    14761 gtatcgtaaa attacaagat atcaagagga tgatttactg tcatcagtag agatttttaa
    14821 acagaaacta ttatcaaaaa agagaataat ggttttatgt ggacagcagg aagaaaataa
    14881 tttaagtgcc ttattggaag agttctttat taaatataat acaattataa tcaaggataa
    14941 ctattctaat atacacggtg aacactttat taagactgtt ctagtaacag aaagaatgag
    15001 tgtaaatgca tttattgatt attgcccaga tttggtaata actattggta aacatgtatg
    15061 gtcttttgtg aaatatactc ttagagataa aaagcttaaa tttgagcatt ggcggatatc
    15121 tgaggatggt aagattgttg atccgttcaa caagctaaca tctgtctttc agtgctcagc
    15181 ggaatttttt atggagactg tcactgctgg tgtgaatagc aataataatc ttgagtatta
    15241 taatctatgg aagacaagat tatcaaaagt ttgctatcca gagttagtct atagcaattt
    15301 ttatgcaatt tcagaactag taaaaaaaat tccagatagt gcattagttc attctagtat
    15361 tttaaatgcg gctagattgt atgactttgt atctgatgat aaagcattac tcagttttgc
    15421 taatttaggg gctgatggta ttgatggttg tttaccaaca tttttaggtc aggcagaagc
    15481 agataaaact cgacctgcgt atttaatttc aggagacttg agtttctttt atgctatgaa
    15541 tagtttatgt ggtgacattc cagataatgc acatattttc cttattaaca atcattctgg
    15601 aagtgaattc catacaaatt ttggtaagtt ttacaacgga tcattaagtg ttgataagca
    15661 tatagctgca ggacatattt caaacgcaaa ggattgggca ttagaaaata atataaaata
    15721 cattactgcg gattctaagg aaagtctcaa atcggggttg gatgtattta ttaatacatt
    15781 tgacagtcct gttttatttg aggtctttac agatccggat acggatacgt ctgttctgga
    15841 atctttttat agtataaata aggtaatcac accaaaaatg tattttagaa aggcgttaga
    15901 tattataaaa aagcatttac acattaagta gatgtttatt gcgaggtgaa acaaatatgg
    15961 caataaaatc ttatattaaa cggttattgc tttatatccg tcatggagat cccaaaataa
    16021 atttagatgt gagttattta tctgaaggtg caatgcttgc tggtaagact gtgataatta
    16081 cgggtggtgc aaaaggactt ggttttgcaa ttgcaaaatc atttatagct caaggcgcaa
    16141 gcgttattat tacaggaaga gacatggatt cgttgaataa tgctaaatgt cagcttggag
    16201 atatgtgttc tatcagggcg tttgatataa ctgatgctaa aatgtatcga acgttttttg
    16261 aagatgcata cgagaccttt ggacgtattg atatactgct taataatgct gggatctcgt
    16321 tacacgaaga tggatttatg agtgttactg agaaaacttg ggataagcaa atggatacaa
    16381 atttgaaagg tgtttatttc tttacacagg aatttattaa gtttgttaaa gaaaagaatc
    16441 aaatgcaagc aaatattatt atgatgtcct cagaaagagg cttgtgtggt gatagtattc
    16501 cgtatggatt atcaaaggct gcaattaact ctttagttgt aggcttgtct aagaaatata
    16561 tttctgaggg aattagggtt aatggtattg ctcctggagt aactgcaacc gctatgactg
    16621 gtatttctga aaagggtaat ttgtataatc ctgcaacgat cggttataga tattttctcc
    16681 cagaagaagt tgctcaagtt gctttgtttt tggcaagtag tctttcaaac tgtatcagtg
    16741 gtgaaatcat agcctgtgac caggggcaat atttaagtcg aaattggggg taataacttg
    16801 ggaatattat taaaaaaata taatagcctt agtgagcctg caaaagcatc tatttggttt
    16861 acactttgta atgtgttgca aaaaggaatt ctatttttga ctacacctat ttttacaaga
    16921 atgctttcaa ctgcagaata tggtaagtat acgattttta tatcgtggta ttctattata
    16981 gcaattttca caacgttgaa tttatctgca ggtgtctata ataaaggact aattaaatac
    17041 gagaataaat atgcattaac ctcgtcattt ttaggattat cgtcatttat ttccctttgt
    17101 gcattgatgc tataccttcc atttagaacg gttataaatg ggctactgga tttatcgacg
    17161 gagtttgtca tattgattct tattgaatca atatttgagc cggctttttt gctttggtca
    17221 gttaaggaac gatttgatta caagtatcaa aaactagtac tcatcacagt tcttatggct
    17281 gttttaaatc caggtctcgg tattatatta atcttatcat ttggggagta tgctatttgg
    17341 agagtacgag cttatgtcgt ggtaaatgtt gtagtgggga tgtttattgg cataggaata
    17401 ctggctaagg gtaagaaatt atttgataaa gagtactgga catatgcgct cagatttaat
    17461 ttgccattaa taccacatta tttatcaatg acattgctga atcaggttga tcgccttatg
    17521 ataaatagta tggttggagc tagcgaggct gcgatttata gtatcgctta cactatatct
    17581 agtatgatga atatcataac aacagctata aacggttctt ttattccttt tacatacaaa
    17641 gcaattaaag atggcgtgta tgaaaaaatt aaatccgttg ctaatcaact attagtgttg
    17701 atttttgtct tgtgttcgtt tactatgctg ttcgggccag aattgatttg ggttgtcgga
    17761 ggagaaaaat atatggatgc tgtttggatt attcctccag ttgcggcatc agtatttttc
    17821 atgtttttat atccgctttt tggcaatatt gaattttact atgaaaagac aaactacgta
    17881 atgtttgcat cttgtgtggg ctctatcgca aaaattattt caaactatgt tagtattccc
    17941 cgttttggat atatttcggc tggttacacg acattgattt gctatatgtt gttttccttt
    18001 gcccactaca tgttttataa aaaaattctt agagaaaatg gtataaaaga agatatgtat
    18061 aatattaaaa atattatatt tctttctgtc ttaatgattg tgttaactat tggtatgaca
    18121 atattatact tttctacaat tctaagatat agtattatta ttggattagt tattgtggct
    18181 tttcttaaaa ggacgcaaat aatgaaaatc cttaaaaata taaaaaatta acaagcaaat
    18241 cttgggaatg gaacggaggc acggtgaaat gagattattg attccatctg ctaagattgt
    18301 accggaagaa cttcaccatc ttggaaaact tcctgcaatt atctatccga ttaatcaaaa
    18361 aattgtattt gattatttat atgatcaata taaagatgta tgttctgcta tcgatattgc
    18421 ctgttacgaa aagatggata aggtagcaag acgattagat aagtatatta aatctaaaac
    18481 agtaaatatt attcagctta aagagttagg ggatcttgga agaactatat atgattctct
    18541 aataggatgt gacgagcctg ttattattaa ttttgctgat accattatca atgataatat
    18601 atattccttg gaatgtgaca gctttttcta tgctgaagac tattatagta atacttggac
    18661 attctttgaa gaaaaagacg gagatattat ttctgtttta gataagaacg agttaaaaga
    18721 agatgatgga aagaagcaca aactgttttc tggtgtattt caaattatgg atgcccagta
    18781 ttttagagag tgtttgcgta aagctttgat gtcaaatgta gtaaatgtga atagttttta
    18841 tcaagcttta caagagtata gcaaacgata tgaatttctc tctattaaaa caaataattg
    18901 gtttgacatt ggacatgcag ataaatatta taattccaaa ttggaagtta aggcaagaga
    18961 atttaaccac atttcaattg ataaagatcg tagcatcctg agaaaaatta gtgaagatgt
    19021 tgaaaaattt atcggagaaa ttaagtggta tcttaagttg ccagcacagg tagaatatgt
    19081 gagacctaga atattcgaat attctacttc gtatattaat ccatatgtaa gtatggaata
    19141 ctattcgtat catactgttc acgagttatt tctctatagt gacttaacaa aaaaacaatg
    19201 gatagatatc tttaatagaa tacgatttgt atgctcagac tttaagagat actctgtttc
    19261 aggggataat attcaaaagt cattgaaaga tatgtatctc gataaaacat ttcagagatt
    19321 caataagctc agaaaggacc cccggttcac agaattcttt tcttctgata ttcaaataaa
    19381 tggagtcaga tataaatctt tggaccagat agaagcttta ttgagcgtaa gtgttccaag
    19441 agaattgttt gacattacgc aatttaatat tattcatggt gacttatgct ttgccaatat
    19501 tatggtagat aacacatttt cgttcattaa agtcatagat ccgcgaggta agtttggaga
    19561 ttttgatatt tatggtgatt atagatatga actggcaaaa ttgtttcata gtgttgatgg
    19621 aaaatatgac tttataatta aggacttgtt tacaataaaa tatgatccaa agaaggcaat
    19681 catagactat attgttcaag atcgtaaacg agattatgat ttatatgaag tattttactc
    19741 tgtcttcaaa gatgaaatag gttctgattt gaaaaaaatt gaacttattg aggctttgct
    19801 gtttttaagt atgattcctt tgcatggaga aagcttaaac catcagatgg ctatgttagc
    19861 aactggtttg gaaattttag gacgagttgt cccggatatt tattgttaat gctttgagct
    19921 attgtttttc tatttgatca gtgtaaagcc gggcatataa attgcctggc taaaataaag
    19981 gagatgtctt agttatatgt ttaaagatta cgcatttgtt tttgatattg atggaacttt
    20041 gtgccccatc aaaaagaaag atgagaaata tgaagattta gttccgtatg ctaatatggt
    20101 ggagaagttg cgatactata aggagaacgg agcaaagatt acactgttca catctcgcaa
    20161 tatgaattct tatcatggta atttaggtgt gatcaatgca aatactgcaa aaatcttgtt
    20221 ggcttggcta gataagtggg agattccgta tgatgaaatt ctttatggaa agccttggcc
    20281 aggccataaa gggttttatg ttgatgatcg cacagttcgt ccggatgaat ttttatcgtg
    20341 taccctggat cagctcgatg agatttgcaa aaaatccaaa ttgaaatctg agtaaaggag
    20401 actttataaa gtgaataaga tgacggttat tattacaatg gctggtcttg gcagtcgctt
    20461 tcgtaaagtt ggctacactg tgcccaaatt tatgattgaa gcaaaaggta aaaccttatt
    20521 tgattggtcc ttagacagct tgcttgatta taattcacat gtagaaaagt atgtttttgt
    20581 tgtgcgtaag gaagatgatg ctactgtttt tatcaaagaa cattgcagtc tgtatggaat
    20641 tgaaaatgtc gaggtagttg aaattgacta tatgactgat ggacaagcca ctacatgtat
    20701 gcttgcaatt ccttattgcg cacctgataa tgcaattatg gtttataaca tagatacata
    20761 tgttgagcca ggtgagatga aatacgttga tatttctgga gaaggacata ttccgtgttt
    20821 ccatgcggac ggtgatcatt ggagttttgc caagttgaac gaatatggtg atgtagtaga
    20881 agtaagagaa aaagttagaa tttctaacaa ttgtacttta ggtgcatatt atttttcttc
    20941 tgcaaaatta tatgcatcac tctacgaaga atattatgca gatgaaaggc atctggaaaa
    21001 gaatgagaaa tatattgcgc cgctatataa ttatatgata gagaagggca tgaaggtaac
    21061 cattagtata gtagataaat caaaagttca cgtactcggt actccagaag agctcgatgt
    21121 gttcttaaat gaatagggaa aatataatca tagaaaatgt taattataaa gctgcgtgtg
    21181 tgggggagta ctattcgata gatattctaa aaatactaat ggctatttta gttgttgttc
    21241 gtcatttagg tcaaaactat ttagctccag attaagcatt tagtattggt attgttgcag
    21301 gactgagtac ggttgccgtt cctacttttt tcatcatcag ttcctttttg ttgtgtagtg
    21361 gtgaagttac atccgctaaa ttatctaaac aaatctacag agtacttaaa atttacatta
    21421 tttggtctat attatatatt ccattagtat taaagaattg ggtacagtca gattttagct
    21481 ggaaaacaga tgtattattg tatatcagag attttctgtt tattggtagt tactatcacc
    21541 tgtggttctt gcctgcactg gcttttgctc tatggttagt acgcaaaagc caggtttcta
    21601 aaataaagtt tctcagtggt ttgttgctat gcgtagtagg tttagcattt gaatcttatt
    21661 ggtttatatt atctgggaat gattccatcg agtatattag atattgttat aaaagcgtgt
    21721 ttgtaacaac tcgtaatgga ttatttatag gtgtaccatg cgtcattatt gggcaacact
    21781 tagccttcca caaaaacact atacttaata aggtctttaa ataccgtaat tttatcttat
    21841 gtgctgtttt agctatagga tgcttattag ttggtgaaag tttcatcata aatcaaatag
    21901 ctttgcgaga agtcaataat ttgttaatat tttccattcc attagcactg gtagttgtat
    21961 cagtatcact gagcctaaag atagtacatt tataaaaata acagtacatc tgcgaagaat
    22021 tagtacgtta ttattttgtt tacatccgta tataatagct gctttaaatt ttattttccc
    22081 aaaattgggg attacaaatt cattaataga aatgacttgt ggaatgataa taattttaat
    22141 ttctacagtt tttgtgtatg cgtgtagtgt gaaatttacc ttgctgaaaa aattatatta
    22201 gattagtaac tggtacatgt aaatttgttg aaattatagc agcatagaga taggaattct
    22261 gttgagtttt attttcgctg gccgaggtta ttcatgttag agtgggtacc tccttttacg
    22321 aaaccttctt gatggtactc atttagcgca tgatgaaagg agagtttatg aagaaaatca
    22381 tatcttctat tttggttaat gtgttatctg cattgtatca aatatttggg tttcgatttt
    22441 aattgcagtt ttggctatgt ttttatactt gtttgcgatg gctgaggaga aatcaggatt
    22501 aggtttgaaa aaatcttttc ttgtctggat tcggtatttc agaaaatcaa gctcatttag
    22561 aaaaatgttt ttgcttgtgt tttttacaaa tatgattctt tttagaacac tgatggatag
    22621 atatgtttgg ctaaatcctt tgtctaatgt gtttggtagt tggtggattt actctgtgaa
    22681 tgaaacgaca ggggagatta gattaactac agaatgtttt gaaaatgtta ttctgtttat
    22741 tccatttata attctagtaa tgtggtataa ggcttcagaa aaagattttg gaaaaattta
    22801 tcagagatta taaaatttgt ttttttatgt tcgtgtctta tagagttttt gcagttattt
    22861 ttacacatag agacattcca attgtcagat ttattttata atactttggg tggcggcatt
    22921 agtggggtta tatattattt gagctgatat attcatccta accttaaaaa taaatgagca
    22981 gaattttaaa agattggaga gattctatgg atcataatgc aaattattat cattggctta
    23041 caaatgccaa tgcagaaatt gtagaggaac tgcggtcata ttccgagaag gacattgagg
    23101 actccttcta taaaaatctg tcctttggta ccggtggtct gcgtggcacc attggtgcag
    23161 gcactaatcg tatgaacgta cacaccgtag gtaaggcaag ccagggactg tcagactatc
    23221 tgaataagac ctatgaaacc ccttctgtcg tcgtgggcta tgattcccga atcaagtccg
    23281 atgtatttgc aaaggttgcc gcggacatct ttgctgcaaa cggtattcag gtgcatctgt
    23341 ggccggagct gctgcctgtg cctaccgtat cctttgctat ccgttatctg aaggcttctg
    23401 ccggtgttat gatcaccgca tcccataatc cttccaagta taacggatat aaggtctatg
    23461 gcgcaggcgg ctgccagatc accaccgagg ctgccgctga gattctggct gaaattgaaa
    23521 agctggatat ttttgcagat gtgaaaatgg gcggcgacgc ttccagaatc gcttctatcc
    23581 agccagaggt tctgactgct ttcattgaag aagtaaaggg ccagtcggtt ctgtttggcg
    23641 aagaagtgga caagaatgtt gctatcgtgt attctccttt gaatggtacc ggtctggtgc
    23701 ctgttacccg tactctggca gaaatgggct acagcaatgt gaccgttgtc gaagagcagc
    23761 gtctgccaga cggtaatttc ccgacctgtc cttatccgaa tccggaaatt aatgaagcta
    23821 tgtctctggg catggagtat gccaagaagt ataatgctga tctgctgctg gctaccgacc
    23881 ctgactgcga ccgtgtgggt atcgccgtga agaatgcaga aggggattat gtcctgctgt
    23941 ccggtaacga aactggtctg ttgcttctgg actacatatg ctctcagaga attaagcacg
    24001 gcaagatgcc cactgaccct gtgatggtca agaccatcgt gactatggat ctgggcgagc
    24061 ggatcgcttc caactatggc gtgcgtactg tcgatgtgtt gaccggcttc aagttcatcg
    24121 gcgaacagat tggtatgctg gaaaaggcag gcaaggtgga cagctatatt ttcggctttg
    24181 aggaatccta cggctatcta tccggcagct atgtccgtga caaggatggt gtcaacgccg
    24241 cttatctgat ctgcgagatg ttctcttact acaagactca gggtatcagt ctgtttgata
    24301 agctgaacga gatttatgct aagtatggct actgtttgaa caccctgcat tcctttgagt
    24361 ttgagggtgc ggcaggtttt accaagatgc agaaaattat ggttgaattc cataaaggtc
    24421 ttgatatgat tggaggtaag aaggtcattg aaaccttgga ttacagcacg ggactgaatg
    24481 gcctgccgaa gtctgatgtt ctcaaatatc tgctggaggg caactgctct gtagtggttc
    24541 ggccttccgg cactgagccg aagctgaaga cctatatcag cgttagcgca gaaagcaagg
    24601 aagctgccga agtaatggaa gctgttatgg ctgctgaact ggctgaaatg tttaagtaat
    24661 actatgaaaa agatctgagt gtcaatgtcc gaagtagcaa catctatgtt taataaaaat
    24721 ttcctgcttt acggttctgt ttacataatt gaggtaaagg ggccgactgt gtggatcgaa
    24781 aaggcaaaga agtgttaaca gagacacagc agcctgatga agcaccgatc aaagcagtaa
    24841 gggagaaata agagatatta gagttttaat aatgtattca gcaatagaga tgaaaggagt
    24901 agaatatgaa taaaactatt gaaataataa aatgaggggg aaagaaataa acgtgatatt
    24961 attagttttg agaaggcgtt ttcaatattc gctattgtat tgatgtacta ttttcagcaa
    25021 tcgtatttgc cagcaatact gaacaaagca atatctattg gtgatacagg agtgcatata
    25081 ttcttctttt gctcaggttt tgggctatat ctgtcatatt taaaaaagcc tattacattg
    25141 ggtgaatttt tacagaaacg atttatcaag atttatattc cgtatattat cgtaattacg
    25201 gtatcagtgt tactacctta tatgacacaa gatttaaatg ctgcaaaagt atatttgtct
    25261 catgttttgt tatttaaaat ttttctacca gtatatgagg agtcactggg tccctttggt
    25321 ttatttcgac aattattcag ttctatttct tattcatacc aattgtaaaa ctatagagaa
    25381 aaataggaat gaaatgattg cttgttgcaa gctgagttat tagtatctgg gctattatga
    25441 tgtttcatac aggatatgga agcatacgag ttcttgggag ctgctttgcg caatatattt
    25501 gggaatttac tttcggcatg tttggttgca gagtatttag tgaaaagaaa aatagtaaga
    25561 tttaataata tattcgtgat tatggcgtcc attgttggaa ttggttttgc tggtatatcg
    25621 aaaattattg gtggactgat ggagtctatt aatgatattt ttgcattctt cggttacggt
    25681 gcaattgcaa ttttaatgta tcaagttatt ccatttagaa agataatgcc taggatttca
    25741 agatactcat atgatatata tctactccat attcttgtat ttagtacgat tcatcaaatt
    25801 atccctaact tggttttggc ggcatttgtt tccattccta taacgttggt gttgtcatat
    25861 tattatagta ttatgttata tagacttaaa ttaaaatgat agcataattg tcgcctctac
    25921 cgggcggttg ccatcctgat taacagcctc cacgcggcgg atggtgggca cttaggtagt
    25981 tcgtaaccta gtggtagatg gttggtaaaa aggagataaa actaaatgat t
//
LOCUS       DACCPY010000035        25416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCPY010000035 DACCPY010000000
VERSION     DACCPY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.035"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(8..1555)
                     /locus_tag="IVO69_001254"
     CDS             complement(8..1555)
                     /locus_tag="IVO69_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7400039.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1657..2010)
                     /gene="tnpB"
                     /locus_tag="IVO69_001255"
     CDS             complement(1657..2010)
                     /gene="tnpB"
                     /locus_tag="IVO69_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7400040.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2000..2194)
                     /locus_tag="IVO69_001256"
     CDS             complement(2000..2194)
                     /locus_tag="IVO69_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400041.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2438..2722)
                     /locus_tag="IVO69_001257"
     CDS             complement(2438..2722)
                     /locus_tag="IVO69_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400042.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(2740..3630)
                     /locus_tag="IVO69_001258"
     CDS             complement(2740..3630)
                     /locus_tag="IVO69_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7400043.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(3650..3868)
                     /locus_tag="IVO69_001259"
     CDS             complement(3650..3868)
                     /locus_tag="IVO69_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400044.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(3838..5538)
                     /locus_tag="IVO69_001260"
     CDS             complement(3838..5538)
                     /locus_tag="IVO69_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7400045.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            5805..6578
                     /locus_tag="IVO69_001261"
     CDS             5805..6578
                     /locus_tag="IVO69_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400046.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(6659..7228)
                     /gene="thiT"
                     /locus_tag="IVO69_001262"
     CDS             complement(6659..7228)
                     /gene="thiT"
                     /locus_tag="IVO69_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7400047.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(7294..7396)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7402..8373)
                     /locus_tag="IVO69_001263"
     CDS             complement(7402..8373)
                     /locus_tag="IVO69_001263"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7400048.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(8467..9840)
                     /gene="glmU"
                     /locus_tag="IVO69_001264"
     CDS             complement(8467..9840)
                     /gene="glmU"
                     /locus_tag="IVO69_001264"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7400049.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(10343..11161)
                     /gene="purR"
                     /locus_tag="IVO69_001265"
     CDS             complement(10343..11161)
                     /gene="purR"
                     /locus_tag="IVO69_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7400050.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(11328..12152)
                     /locus_tag="IVO69_001266"
     CDS             complement(11328..12152)
                     /locus_tag="IVO69_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7400051.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(12125..12805)
                     /locus_tag="IVO69_001267"
     CDS             complement(12125..12805)
                     /locus_tag="IVO69_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7400052.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(12828..13766)
                     /locus_tag="IVO69_001268"
     CDS             complement(12828..13766)
                     /locus_tag="IVO69_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7400053.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(14011..14862)
                     /locus_tag="IVO69_001269"
     CDS             complement(14011..14862)
                     /locus_tag="IVO69_001269"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7400054.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(15016..17589)
                     /gene="mprF"
                     /locus_tag="IVO69_001270"
     CDS             complement(15016..17589)
                     /gene="mprF"
                     /locus_tag="IVO69_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7400055.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            17730..18536
                     /gene="proC"
                     /locus_tag="IVO69_001271"
     CDS             17730..18536
                     /gene="proC"
                     /locus_tag="IVO69_001271"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7400056.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(18615..20171)
                     /gene="rny"
                     /locus_tag="IVO69_001272"
     CDS             complement(18615..20171)
                     /gene="rny"
                     /locus_tag="IVO69_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7400057.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(20582..21631)
                     /gene="recA"
                     /locus_tag="IVO69_001273"
     CDS             complement(20582..21631)
                     /gene="recA"
                     /locus_tag="IVO69_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7400058.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(21740..22981)
                     /locus_tag="IVO69_001274"
     CDS             complement(21740..22981)
                     /locus_tag="IVO69_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7400059.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(23221..23799)
                     /gene="pgsA"
                     /locus_tag="IVO69_001275"
     CDS             complement(23221..23799)
                     /gene="pgsA"
                     /locus_tag="IVO69_001275"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7400060.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(24008..24892)
                     /locus_tag="IVO69_001276"
     CDS             complement(24008..24892)
                     /locus_tag="IVO69_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7400061.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         7215 a         5512 c         4416 g         8273 t
ORIGIN      
        1 ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc aacagttgat
       61 ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga aactctagat
      121 actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga atgattcctg
      181 aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct atcaccagct
      241 ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct tcaaaaatag
      301 gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac ttggattttg
      361 caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga cgcagttctt
      421 gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt tctaggacaa
      481 accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt ttctttcctt
      541 ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca agtcgtacac
      601 tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta aactccttga
      661 gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa acgatgggat
      721 gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg gcactttcaa
      781 tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga tagactctta
      841 atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa ttggtgatat
      901 tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc cgataggcag
      961 gaactttctg accaaatttc atccggatcg tttccgccac taaactattg gaacctaagc
     1021 tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt ccttcttttt
     1081 cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac atagcctgat
     1141 ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt gaaccgcaat
     1201 gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata ggtaagtgtt
     1261 tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga ataatggtgg
     1321 cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc aattgcccat
     1381 tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt aaataattga
     1441 ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt tgttgtgctt
     1501 gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt tccaaaattt
     1561 ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg cacaagaaat
     1621 aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt tttttcctgg
     1681 caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa cctgttggtt
     1741 cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt tttctatccg
     1801 cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac ggtcttgacg
     1861 gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact cttgttgaat
     1921 aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca caatataaat
     1981 atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa atagcttgaa
     2041 gaatataatt gttaataccg ttatagatca aaacttcaga accattctct cttttgatgc
     2101 gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt tcgtttccta
     2161 attggacagg aacaatctca tttaatttat acatagaaaa cctccagact tcttgatagc
     2221 tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc gcttacttaa
     2281 taccgagaaa atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa
     2341 ttttgtcaca acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt
     2401 ctgattattt attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag
     2461 tattcactac acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta
     2521 cctcttctgt gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt
     2581 tgatcaaaat aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa
     2641 agccgttttt ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga
     2701 acgttacgtc ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg
     2761 ttctctgatt ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc
     2821 atttgatttg gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa
     2881 ttcccgacct acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc
     2941 cagacgatcc agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa
     3001 caaccgttgc tcatcgactt tcgctccaag aaattcctcc agcttttttt gataccttat
     3061 ttgaaattcc ttttcataga tttctacaaa actatcgagt ttttttagta cttctttgaa
     3121 ttcttcagta ttctttttca acactaaagc taaagcttga ccttcttttt ctcgactcgc
     3181 ctcaatagaa tcaagtgcgc ttttcaatgt atctagagct aactgtgcca attcttccat
     3241 gctctcttcc ggtttttctt ctaccgttac gaagttttcg tccagcgcga agcgttccgc
     3301 catccgacta attgacactt gattttgact accgtatcgt tgttgaattc cgtctgtcaa
     3361 ttgagtgata agtgaatcaa tcaattccca gttgatgacg atctgttttt cgtttttgcc
     3421 aatataagct agattcacaa atacttcgat tcgaccgcgg cttaactgct gtttaataag
     3481 ctgacgaaaa tcgttttcaa caaagttcag gattttagga cttcgtattt gaacatctag
     3541 aaaacgctgg ttgacacttt ttatttcaat atctaactga tacgcttttg tttcgcggat
     3601 ggctttgcca aaaccagtca tacttttcat tcgacagggt ccttcctcat tacatatttt
     3661 ctattgcttt ttgcagttgt tcaaactctt cgttcgtcaa tgtcaggcct ttccccattt
     3721 tttcatggtc tggcgcccaa tctcgaagat cgaattttgg cgggcgacca ttccagctga
     3781 ctaggttcag ttctttacgc caccccttgt tgttttctga caatatagcg atttcttcta
     3841 cgatttcata tgaaaattct tgtgccatgg ttgttcctcc ttgagataat ataaatatag
     3901 gatcagtaat gtgtcaactg gttgcttaag caattcatgc tgcaaacgat ccttgcgttt
     3961 tccttgtagc agataatcaa aataaacagt tttcgtgcgc tcattttgta ttttttgctg
     4021 gtattttcga taccttcgcc attcttcagt acttaattga ccaatttgtg ccaataaatc
     4081 atccggcata ctttttatat cttgttggca gatcatttga atcatcatgt ctacttgata
     4141 gatttcattg ggcagtaaaa aatgtttttt tgctaaaagt tctagcatcg ccaatccgca
     4201 tgtctctggt acgaccctgt tcttatataa attacgcagt ttttgccaaa attttatgct
     4261 tttgaaaaaa gataattcta actgaatttc ttttgttaag taagacaaaa atcctcgcca
     4321 gtatgttctt gcaggtttaa cagaatgaaa tgtataccag ttcccatagt aatagcctaa
     4381 tcgttttttc atttccagat agcctaattg taaatttaat tggttccttt ggctatcaaa
     4441 aagcagaaag ctgcctagtg tccacaatgt ttgacatttc caatggataa aaatgtctgg
     4501 catacgaatt tttttcgctg ggcctggccc ttgcacatct acgacaaatg cctctgttgc
     4561 tcctttgtta atcgcaatat caataggtat tttgttgcga tacccaccgt ctgcatattt
     4621 attttttccg atctttcgat aagccatagc gggataaaat gaagcagagg cgagtatcca
     4681 atctggtatt tcttctgttt ttgtctgttg gatatcagtc actacttccg ctagttctgg
     4741 cagtttagta gacaccgtat ataaacgtat tttgggatta tgtttgagta aggacaaatc
     4801 caaattgtct ttgaccatct tttccaatgg agcagaagaa accccgcctt caattagtgc
     4861 tgttttcgtc atttggcgtc tttctcgata gaagcgttca cgaaggtctt caactgcagc
     4921 catttctggt aacgcaagca cttgtcttgt cgacagcttt ttccataaat tcacagcttt
     4981 tttcatatct ccttgtaaaa tcaatgcgcc gttcaacgca cctaccgagg tacctgtgat
     5041 aatctcaaag ttgatatcat gttccttcag cgcctgccac acaccaatct gataagcacc
     5101 gtgcgctcct cctccaccta aaacaagtgc tgtatgatag accagctttt tatttaattg
     5161 tgtggttcct tgatagccgt ttttatgaaa aatacgcaac cattcttctg atagaggaga
     5221 ttgaaataaa aaacaacagt tttctttaat aaaaaaacga gcgcaagctt ccatataccg
     5281 aaaaaatttt ttccattcaa aagtttcacc ttcaatcaac agattattaa aaataacatc
     5341 tcctctactt ttagcaatag taaaatagac ggtatatttt ttcgatactg ctttaaaagc
     5401 acattgcttt tttgcagaac gccaaaaagc gtctctactt tcttgatgac tttgaaaaaa
     5461 tggaaggggt tgttttgctt gctggatagg cgaatacagc gacattttct gtgaaaagta
     5521 aacttttttt atctccatcg cgctctcctt tttcttcaag tataccagta cgtcataaaa
     5581 aggtcatgag tctcgtattc gactttgtag attccataag aaaacaattc ataaaacctc
     5641 ctttaaataa aaaaatacaa aaatcacaaa aattaattga aaatgagagt gttttcaatg
     5701 aaaacgtgat tttgtaatgg aattttaaaa taaaagaaac aagctttttt aatgcaaatg
     5761 ttgtaaaata aaagagatta agaggaaata agaggaggaa catgatgact gagtcagttt
     5821 atgtacatat tgataccaca agtaatgctg ttttaacaaa agggctgact gctactgact
     5881 ttgcaaacag tatcgtgcat tttcctcaaa acttgctctt gcttgatcca tctgcttctg
     5941 ctggagaata tgaatcccat acagggctga aagtaatccg tggtacagaa aatattcagc
     6001 gtttcttttc ctcttctcgt aacaggcgtg gattcgatga attgaagtgg atcgacttta
     6061 ctgatttaac gatgctgaaa gaattgacac ctttagaaat ttcagaatta ttatattttg
     6121 gtcatatgaa aacacatctt cattcgccgt ttttttataa actacagaat aattttgtat
     6181 acttcgatct taatgatcag ctgaatcgga tttattatcg ctatttagat gaattttata
     6241 ggatttttgc tagtaaatta agttcgatct tgtcagataa gatcaatgta aaaaaatctt
     6301 ttttcaaaaa agaaacacct gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta
     6361 atttgatgca ggaagggatc attttttctt ttgatcaagt tggtttgatc gatggtaaat
     6421 atcgtatccc aatctatgtg attgaagatt atatttggaa attaaaaggc acccggtatc
     6481 gagaagagga aatcatcgct actttaatct actctgtcac cgatcgacac tggcaattgg
     6541 tagaagatgc cgatctttct tatctgccat tgaagtagat ctatttctta tcaaaaaata
     6601 aaaaggaaac aagaaactag gacatgattc tgtctcaagt ttcttgcttc ttttttattt
     6661 acagctcctg tcgtttatct ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata
     6721 aaacgatgat cgttacaata gctgtactca acccgctggc accattcatt acgaaagaaa
     6781 agagccaagg attcatcccc catagtgcat acgctcccca gaaaaccact cctgcaataa
     6841 aatgccaaaa atatctggaa agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa
     6901 tttgtttcgt attatcgtct acatagcctt tttggacatt tgaagccatg agccctgctg
     6961 cgcctgcaaa agtataggca atcgggtact caatcaacac ttgaacgaca cttaaataat
     7021 aaacttgtcc caacggaaaa tgtaggatgc cccaaatgaa acctgccagc atccctgcct
     7081 ttgtccctct tcttaacgca aaaagagtca atggaatctg ccctaatgaa atagaaaaac
     7141 tgctgccgat ctggatcgga atcaacgaca acaccatcgc taacgctgca acaatcgttc
     7201 cttctaccca aactctattt tttcccataa aaaaacttcc tcctttgaat aaaaaagtca
     7261 caaaggagga agcagctttc gaaaatagtc aaacagctgt ttattcacaa ttcctacgct
     7321 cgcataaccg atacaggtac gaagggttta gaaatagatt caatctcagc ccaattggac
     7381 tcccctttgt gagtttattt attatttatt ttttgtttca aacaatggac taactggttt
     7441 gttttcatgg atacgtttga ttgcatctcc cattagtccg ccaacactta cttcatcgat
     7501 tttgtcgatt ttacgatctt ctggaagata gatcgagtct gtaacaacta gacgctcgat
     7561 agctgaatct tctattcttt gtaaagcagg gccggataat actggatgtg tacatgaagc
     7621 atacacacta gttgcaccag cttctttcaa agcattggca gccaatgtga tcgtacctgc
     7681 tgtatcgatc atatcatcga tcagtacaca agttttgcct ttgacttgtc caatgatatt
     7741 cattacttct gcaacatttg cttttggacg gcgtttgtct atgatcgcaa tcggagattt
     7801 caaaaattcc gctaatttac gtgcccgtgt gacaccacca tggtctggtg aaacaacgac
     7861 aacatcatcg cctttgattc ctttttctaa gaaataattg gcaataagtg gcgcacccat
     7921 caaatgatcg actggaatat caaagaaacc ttggatttga actgcatgaa ggtccaaagt
     7981 cagcattctt gttgcaccag ctttttgaat catgtttgct actaatttcg ctgtgattgg
     8041 ttcccgtgca cgtgctttac ggtcttgacg cgcatagcca taataaggca tgactacgtt
     8101 gattgttttt gcacttgctc gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt
     8161 atcattgact gggctgctcg ttgattgaat aacatagaca tgcgatccac gaatactttc
     8221 ttcaatgttt acttgaattt ctccatcact aaattgagta acagatgatt ttcctaactc
     8281 gacgccgact gctgctgcaa ttttctctgc taatgggcga ttggaattta acgcaaaaat
     8341 ttttaatctt gggtcaaagt aatgttttga catggggacc tccactttct ttttcacaac
     8401 tctagaattc tacctaataa atagtagtca tattttttaa ataaatcaag tgattttgca
     8461 aaagtcttag tttagataag ggagtttttt tgcatatcct tctttattga cttgtcttgc
     8521 tctcgcaatt gccagcgcat attctggaat attgtctgta attgttgacc cggcagcaat
     8581 cgaactgttt ttagctacct cgacaggtcc gatgatattc gtgctagaac caataaagct
     8641 gtggtcgccg acagtcgtat gatgtttgtt ctttccatca tagttgacga agacaacacc
     8701 acatccgaca ttgatttctt ctcccagcgt agcatcacca acataagtca agtgaccgac
     8761 tttagttcct ttgccgattt gagcattttt cacttccaca aagtttccga tatggacgtt
     8821 ttcgccaatt tcagcttttg gtcgtaaatg tgcataaggt cccacatctg catgtttctt
     8881 gacatggctg ctttcaatga ctgagttttc aatgacgacg tgatcctcaa tcctactgtc
     8941 tacgattttt gaatgtgacc cgatgacaca atcagaacca atgactgtat ttccttggat
     9001 ttgtacacct gcttcgatca atgtgtctgg accgatttct acgcctgcat cgatgtatgt
     9061 cgttgctgaa tcgacaaagc ttacgccatt gaccatatgc atctggttaa ttcgctttct
     9121 catgatctca tttgctttag ctaacgcgat ccgatcattg actcccattg attcttcaaa
     9181 atcatccgtt tgataagcag caactgtatg gccttcttct tttaagattt cgatgatatc
     9241 tgttagatag tattcacctt gagcattgtt tgttccaact ttgcttaacg catcgaacaa
     9301 agcttcgttg tcaaaacagt aagttcctgt attgatttct tgtacaagag cttcttctgg
     9361 tgttgcatct ttttgttcca cgattttttc tacgatccca atatggtcac ggatgattcg
     9421 tccatatcct gttgggtttt ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc
     9481 ttggtgatat tcaaaaagat tgttcagcgt ttcagtagtc aataatggtg tatcgccact
     9541 gataactaac gttgttcctt tttttccttc caagaaagaa gcagcttgca aaacagcatg
     9601 tcctgttccc aattgttctg cttgtaacgc atatttactg cgttctccta gttgtgcttt
     9661 aacttgttct gctccgtgtc caacgatcgt aatcacttca tctggtttcg tttcactgac
     9721 ccgattaatg atatgttcta ccataggttt accagaaacg ggatgtaaaa ctttatataa
     9781 ttttgacttc atgcgggtac ctttccctgc cgccaaaata attgcataac gtgcgtccaa
     9841 ctgcgtcact cctaaatata gtttgctgat ttcagtttat cccagtatac ccattttttc
     9901 aacaaaacaa gtgattttct tttatcccag cgttttctgg ataaaaaaat agatagacca
     9961 taacgggcct atctatttgg taaccggtgc tctcctgcca actcctcggt cacaagtcac
    10021 actactcata aaacatgaaa agctgttttt tgaaatcgtt tcttgtgact tagagttaaa
    10081 cggtaggctt acaacctctt cattaaacgt ttttcaatca gctgaccttc ggtattagct
    10141 caataaacgg caaaatatga ggagcaattt ttccgtttat tgagctaata ctcaggacag
    10201 gacgctgatt tcacaacctc tagataaacg gtgttcaaaa gccagtttct cggtattaag
    10261 ccaaatttat gaaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac
    10321 taagcagctt tttcacaacc tcttattctg aaaaataatt tcctggcaca accgtaatat
    10381 ttttcgtttg agtatctaca tctttgacat aaagcagtga cgtataatca tcgatggcgc
    10441 gatgaccgtt gaattttgat tcggcaaata cagtgatgcc aacaagttct gattcgaatt
    10501 cttcaatcaa gctcttcatt ccgttgactg ttccgccgcc tttcatgaaa tcatccacta
    10561 caaggacacg tgaaccacgt ttcaaacttc tcttagacag ttccatcttt tcaattcttt
    10621 cagaagatcc agacacatag ttgacactta ctgttgatcc ttctgttatt tttgagtccc
    10681 ggcgtacaat aacgaatggc gcattcagat aataggacac agcttgggca ataggtacgc
    10741 cttttgtggc tacagtcatt acagcatcga ttttttcacc aaggtattta gaagcaatga
    10801 ttcttccaac ttgacgcagc agttcaggtt ctcctaacaa atcagagaga taaacatatc
    10861 ctcctggaag cagacggtct tgttctgata gacgttctgc tagagattca atatattttt
    10921 tcgcctcttc atatgaaatt tcagggatga acaatacacc acctgcagca cccgggattg
    10981 tttccaaggt tccataccct cgttctttaa atgttttctt gatgattccc aaatcttcac
    11041 tgatagaaga tttagcagat tcataacgtt cggcaaagct tgttaaggaa atcaattcat
    11101 gcgggtgatt caataaatac tgtgtcatgt caactagacg ttcactgcga cgtactttca
    11161 cctattccaa ctccttttaa gaaattttat cgttttacat tatataagtt tttcttcaaa
    11221 aattcgagca ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa
    11281 gcctataaaa aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt
    11341 cttctgaagg caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag
    11401 actaatgtga ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac
    11461 agcatcccta gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct
    11521 aatcgcatac cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca
    11581 atcgggatca tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa
    11641 tgaacgggta gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc
    11701 cgtttgaata aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact
    11761 tgctcccatg taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa
    11821 ttccctccgc tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg
    11881 gcaatggaga tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct
    11941 gctaatacaa cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac
    12001 gctacccctg ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc
    12061 actaaaaaca cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct
    12121 cttctcatga attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga
    12181 tctgtctgtc cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt
    12241 tcccatggac atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat
    12301 ccatacctgt tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg
    12361 ctaaactgat tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat
    12421 cccacatccc tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac
    12481 gcttgaacca gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag
    12541 gaaaacctgc attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc
    12601 cttcttgatt tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg
    12661 atgctttcac caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac
    12721 ccgcatctac atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca
    12781 aatcagatat ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt
    12841 tcttttaaag ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt
    12901 tgttcattag tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc
    12961 gacaatgtct cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg
    13021 tttttcttca caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt
    13081 gaaacgcccg aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca
    13141 gcatgctgag tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga
    13201 tccagtgctt caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta
    13261 tctggatatt tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc
    13321 ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct
    13381 tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc
    13441 aaggtctgtt tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa
    13501 ctattataca cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg
    13561 aaatcatgcg gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg
    13621 atctcttttg taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt
    13681 ttgtctgctt ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc
    13741 gtagctgtca aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt
    13801 tacgatttat taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca
    13861 tcaatttctg atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc
    13921 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
    13981 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
    14041 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
    14101 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
    14161 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
    14221 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
    14281 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
    14341 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
    14401 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
    14461 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
    14521 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
    14581 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
    14641 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
    14701 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
    14761 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
    14821 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
    14881 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
    14941 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
    15001 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
    15061 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
    15121 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
    15181 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
    15241 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
    15301 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
    15361 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
    15421 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
    15481 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
    15541 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
    15601 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
    15661 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
    15721 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
    15781 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
    15841 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
    15901 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
    15961 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
    16021 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
    16081 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
    16141 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
    16201 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
    16261 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
    16321 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
    16381 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
    16441 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
    16501 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
    16561 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
    16621 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
    16681 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
    16741 tactgctaca tcttgccgaa caggcaattg actcattcct aagatcatca aaacgtcaaa
    16801 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
    16861 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
    16921 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
    16981 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
    17041 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
    17101 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
    17161 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
    17221 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
    17281 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
    17341 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
    17401 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
    17461 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
    17521 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
    17581 atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct
    17641 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
    17701 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
    17761 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
    17821 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
    17881 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
    17941 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
    18001 tttctattgc ttcaggaaaa tcattaacag atttagaaac agcgcttgaa gatcctcagg
    18061 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
    18121 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
    18181 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
    18241 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
    18301 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
    18361 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
    18421 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
    18481 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
    18541 agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc
    18601 ttttgtattt tttattattt agcataatca gttgcacgag tttcccgaat aacgattact
    18661 ttgatgtgtc caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa
    18721 cgaacagcgt ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca
    18781 gcttgaactg cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt
    18841 cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag
    18901 agcgcatctg ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga
    18961 tgagaagcga tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca
    19021 ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc
    19081 aacccggcac gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct
    19141 aattttgcaa cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc
    19201 agacgtccga tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct
    19261 gcctcttccc cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt
    19321 tcttcgatac gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt
    19381 gcgatttctc ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg
    19441 atgattaaat cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca
    19501 atgatacgac ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa
    19561 acttgatctg ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct
    19621 gcttcttctt tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc
    19681 tcttcttcag tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt
    19741 ttttctagtt ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca
    19801 attaattgct gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt
    19861 tctaacgagt catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga
    19921 gattttaatt ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga
    19981 ttttcttcct tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca
    20041 ctatcgatga ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac
    20101 tttgcgacca taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg
    20161 ttgaatacca taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt
    20221 ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac
    20281 atgcacaact ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa
    20341 cggctttcta cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata
    20401 ggtaaacctt taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat
    20461 ttttatttta tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa
    20521 aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt
    20581 tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt
    20641 cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat
    20701 gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag
    20761 aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc
    20821 cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg
    20881 ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt
    20941 tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc
    21001 ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga
    21061 tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag
    21121 acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc
    21181 gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta
    21241 aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat
    21301 cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga
    21361 aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt
    21421 tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac
    21481 ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt
    21541 tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag
    21601 catctaaggc tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc
    21661 agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc
    21721 gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt
    21781 gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa
    21841 ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg
    21901 tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg
    21961 cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct gctaatagct
    22021 ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg
    22081 gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca
    22141 aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg
    22201 ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt
    22261 cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc
    22321 gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa
    22381 tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt
    22441 tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca
    22501 caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg
    22561 tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga
    22621 taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg
    22681 ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag
    22741 ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt
    22801 cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa
    22861 ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat
    22921 tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca
    22981 ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga
    23041 gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct
    23101 cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga
    23161 aattatccct tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac
    23221 ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat
    23281 agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa
    23341 tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt
    23401 tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt
    23461 gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc
    23521 aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt
    23581 gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat
    23641 actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc
    23701 tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat
    23761 acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc
    23821 tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt
    23881 attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac
    23941 acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt
    24001 tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt
    24061 cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac
    24121 caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg
    24181 ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac catacacgat
    24241 tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg
    24301 cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg
    24361 ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct
    24421 cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac
    24481 gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata
    24541 aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt
    24601 ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc
    24661 ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt
    24721 gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga
    24781 ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt
    24841 ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat
    24901 cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt
    24961 gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg
    25021 ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt
    25081 gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
    25141 tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta
    25201 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc
    25261 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt
    25321 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt
    25381 gttccagaag tagcttcttc ggcaataagc cgaaat
//
LOCUS       DACCPY010000036        25313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCPY010000036 DACCPY010000000
VERSION     DACCPY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.036"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(87..464)
                     /locus_tag="IVO69_001277"
     CDS             complement(87..464)
                     /locus_tag="IVO69_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400062.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            720..1448
                     /locus_tag="IVO69_001278"
     CDS             720..1448
                     /locus_tag="IVO69_001278"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7400063.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1448..1702
                     /gene="purS"
                     /locus_tag="IVO69_001279"
     CDS             1448..1702
                     /gene="purS"
                     /locus_tag="IVO69_001279"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7400064.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1704..2375
                     /gene="purQ"
                     /locus_tag="IVO69_001280"
     CDS             1704..2375
                     /gene="purQ"
                     /locus_tag="IVO69_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7400065.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2376..4598
                     /gene="purL"
                     /locus_tag="IVO69_001281"
     CDS             2376..4598
                     /gene="purL"
                     /locus_tag="IVO69_001281"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7400066.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4583..6022
                     /locus_tag="IVO69_001282"
     CDS             4583..6022
                     /locus_tag="IVO69_001282"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7400067.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6054..7097
                     /locus_tag="IVO69_001283"
     CDS             6054..7097
                     /locus_tag="IVO69_001283"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7400068.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7094..7672
                     /gene="purN"
                     /locus_tag="IVO69_001284"
     CDS             7094..7672
                     /gene="purN"
                     /locus_tag="IVO69_001284"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7400069.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7669..9204
                     /gene="purH"
                     /locus_tag="IVO69_001285"
     CDS             7669..9204
                     /gene="purH"
                     /locus_tag="IVO69_001285"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7400070.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9220..10494
                     /gene="purD"
                     /locus_tag="IVO69_001286"
     CDS             9220..10494
                     /gene="purD"
                     /locus_tag="IVO69_001286"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7400071.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10616..11530
                     /locus_tag="IVO69_001287"
     CDS             10616..11530
                     /locus_tag="IVO69_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7400072.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11663..13228)
                     /locus_tag="IVO69_001288"
     CDS             complement(11663..13228)
                     /locus_tag="IVO69_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7400073.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13221..14672)
                     /locus_tag="IVO69_001289"
     CDS             complement(13221..14672)
                     /locus_tag="IVO69_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7400074.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14686..15702)
                     /locus_tag="IVO69_001290"
     CDS             complement(14686..15702)
                     /locus_tag="IVO69_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7400075.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15922..17010
                     /locus_tag="IVO69_001291"
     CDS             15922..17010
                     /locus_tag="IVO69_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7400076.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17064..18590
                     /locus_tag="IVO69_001292"
     CDS             17064..18590
                     /locus_tag="IVO69_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7400077.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18592..19797
                     /locus_tag="IVO69_001293"
     CDS             18592..19797
                     /locus_tag="IVO69_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400078.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20054..21031
                     /gene="guaC"
                     /locus_tag="IVO69_001294"
     CDS             20054..21031
                     /gene="guaC"
                     /locus_tag="IVO69_001294"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7400079.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21295..22719
                     /locus_tag="IVO69_001295"
     CDS             21295..22719
                     /locus_tag="IVO69_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7400080.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23180..24289
                     /gene="pdhA"
                     /locus_tag="IVO69_001296"
     CDS             23180..24289
                     /gene="pdhA"
                     /locus_tag="IVO69_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7400081.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24292..25269
                     /locus_tag="IVO69_001297"
     CDS             24292..25269
                     /locus_tag="IVO69_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7400082.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8190 a         4308 c         5686 g         7129 t
ORIGIN      
        1 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
       61 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
      121 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
      181 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
      241 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
      301 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
      361 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
      421 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
      481 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
      541 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
      601 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
      661 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
      721 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
      781 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
      841 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
      901 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
      961 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
     1021 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
     1081 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
     1141 aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
     1201 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
     1261 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
     1321 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
     1381 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
     1441 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
     1501 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
     1561 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
     1621 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
     1681 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
     1741 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
     1801 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
     1861 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
     1921 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
     1981 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
     2041 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
     2101 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
     2161 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
     2221 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
     2281 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
     2341 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
     2401 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
     2461 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
     2521 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
     2581 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
     2641 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
     2701 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
     2761 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
     2821 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
     2881 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
     2941 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
     3001 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
     3061 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
     3121 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
     3181 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
     3241 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
     3301 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
     3361 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
     3421 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
     3481 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
     3541 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
     3601 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
     3661 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
     3721 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
     3781 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
     3841 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
     3901 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
     3961 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
     4021 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
     4081 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
     4141 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
     4201 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
     4261 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
     4321 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
     4381 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
     4441 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
     4501 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
     4561 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
     4621 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
     4681 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
     4741 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
     4801 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
     4861 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
     4921 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
     4981 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
     5041 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
     5101 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
     5161 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
     5221 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
     5281 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
     5341 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
     5401 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
     5461 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
     5521 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
     5581 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
     5641 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
     5701 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
     5761 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
     5821 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
     5881 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
     5941 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
     6001 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
     6061 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
     6121 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
     6181 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
     6241 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
     6301 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
     6361 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
     6421 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
     6481 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
     6541 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
     6601 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
     6661 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
     6721 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
     6781 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
     6841 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
     6901 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
     6961 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
     7021 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
     7081 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
     7141 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
     7201 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
     7261 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
     7321 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
     7381 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
     7441 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
     7501 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
     7561 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
     7621 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
     7681 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
     7741 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
     7801 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
     7861 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
     7921 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
     7981 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
     8041 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
     8101 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
     8161 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
     8221 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
     8281 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
     8341 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
     8401 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
     8461 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
     8521 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
     8581 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
     8641 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
     8701 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
     8761 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
     8821 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
     8881 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
     8941 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
     9001 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
     9061 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
     9121 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
     9181 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
     9241 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
     9301 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
     9361 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
     9421 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
     9481 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
     9541 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
     9601 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
     9661 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
     9721 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
     9781 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
     9841 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
     9901 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
     9961 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    10021 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    10081 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    10141 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    10201 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    10261 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    10321 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    10381 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    10441 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    10501 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    10561 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    10621 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    10681 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    10741 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    10801 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    10861 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    10921 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    10981 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    11041 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    11101 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    11161 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    11221 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    11281 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    11341 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    11401 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    11461 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    11521 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    11581 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    11641 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    11701 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    11761 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    11821 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    11881 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    11941 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    12001 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    12061 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    12121 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    12181 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    12241 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    12301 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    12361 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    12421 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    12481 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    12541 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    12601 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    12661 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    12721 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    12781 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    12841 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    12901 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    12961 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    13021 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    13081 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    13141 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    13201 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    13261 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    13321 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    13381 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    13441 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg
    13501 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    13561 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    13621 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    13681 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    13741 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    13801 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    13861 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    13921 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    13981 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    14041 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    14101 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    14161 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    14221 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    14281 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    14341 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    14401 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    14461 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    14521 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    14581 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    14641 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    14701 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    14761 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    14821 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    14881 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    14941 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    15001 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    15061 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    15121 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    15181 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    15241 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    15301 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    15361 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    15421 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    15481 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    15541 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    15601 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    15661 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    15721 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    15781 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    15841 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    15901 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    15961 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    16021 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    16081 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    16141 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    16201 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    16261 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    16321 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    16381 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    16441 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    16501 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    16561 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    16621 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    16681 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    16741 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    16801 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    16861 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    16921 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    16981 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    17041 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    17101 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    17161 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    17221 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    17281 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    17341 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    17401 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    17461 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    17521 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    17581 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    17641 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    17701 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    17761 agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    17821 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    17881 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    17941 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    18001 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    18061 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    18121 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    18181 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    18241 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    18301 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    18361 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    18421 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    18481 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    18541 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    18601 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    18661 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    18721 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    18781 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    18841 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    18901 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    18961 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    19021 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    19081 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    19141 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    19201 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    19261 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    19321 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    19381 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    19441 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    19501 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    19561 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    19621 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    19681 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    19741 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    19801 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    19861 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    19921 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    19981 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    20041 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    20101 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    20161 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    20221 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    20281 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    20341 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    20401 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    20461 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    20521 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    20581 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    20641 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    20701 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    20761 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    20821 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    20881 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    20941 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    21001 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    21061 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    21121 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    21181 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    21241 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    21301 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    21361 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    21421 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    21481 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    21541 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    21601 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    21661 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    21721 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    21781 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    21841 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    21901 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    21961 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    22021 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    22081 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    22141 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    22201 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    22261 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    22321 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    22381 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    22441 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    22501 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    22561 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    22621 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    22681 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    22741 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    22801 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    22861 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatagttg
    22921 taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg
    22981 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    23041 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    23101 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    23161 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    23221 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    23281 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    23341 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    23401 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    23461 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    23521 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    23581 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    23641 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    23701 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    23761 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    23821 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    23881 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    23941 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    24001 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    24061 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    24121 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    24181 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    24241 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    24301 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    24361 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    24421 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    24481 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    24541 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    24601 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    24661 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    24721 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    24781 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    24841 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    24901 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    24961 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    25021 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    25081 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    25141 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    25201 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    25261 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACCPY010000037        24991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCPY010000037 DACCPY010000000
VERSION     DACCPY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.037"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(461..1243)
                     /locus_tag="IVO69_001298"
     CDS             complement(461..1243)
                     /locus_tag="IVO69_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400083.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1308..2144)
                     /locus_tag="IVO69_001299"
     CDS             complement(1308..2144)
                     /locus_tag="IVO69_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400084.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2162..3571)
                     /locus_tag="IVO69_001300"
     CDS             complement(2162..3571)
                     /locus_tag="IVO69_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7400085.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3585..5408)
                     /locus_tag="IVO69_001301"
     CDS             complement(3585..5408)
                     /locus_tag="IVO69_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400086.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6251..6577)
                     /locus_tag="IVO69_001302"
     CDS             complement(6251..6577)
                     /locus_tag="IVO69_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400087.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6623..6985)
                     /locus_tag="IVO69_001303"
     CDS             complement(6623..6985)
                     /locus_tag="IVO69_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7400088.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7022..7237)
                     /locus_tag="IVO69_001304"
     CDS             complement(7022..7237)
                     /locus_tag="IVO69_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400089.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7364..7699
                     /locus_tag="IVO69_001305"
     CDS             7364..7699
                     /locus_tag="IVO69_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400090.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7775..9772)
                     /locus_tag="IVO69_001306"
     CDS             complement(7775..9772)
                     /locus_tag="IVO69_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400091.1"
                     /translation="METLMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9870..10145)
                     /locus_tag="IVO69_001307"
     CDS             complement(9870..10145)
                     /locus_tag="IVO69_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7400092.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10378..11112
                     /locus_tag="IVO69_001308"
     CDS             10378..11112
                     /locus_tag="IVO69_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400093.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11151..12293
                     /locus_tag="IVO69_001309"
     CDS             11151..12293
                     /locus_tag="IVO69_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7400094.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12388..13779)
                     /gene="sufB"
                     /locus_tag="IVO69_001310"
     CDS             complement(12388..13779)
                     /gene="sufB"
                     /locus_tag="IVO69_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7400095.1"
                     /translation="MGVPELEEYEFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13784..14254)
                     /locus_tag="IVO69_001311"
     CDS             complement(13784..14254)
                     /locus_tag="IVO69_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7400096.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14241..15476)
                     /locus_tag="IVO69_001312"
     CDS             complement(14241..15476)
                     /locus_tag="IVO69_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7400097.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15473..16759)
                     /gene="sufD"
                     /locus_tag="IVO69_001313"
     CDS             complement(15473..16759)
                     /gene="sufD"
                     /locus_tag="IVO69_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7400098.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16772..17545)
                     /gene="sufC"
                     /locus_tag="IVO69_001314"
     CDS             complement(16772..17545)
                     /gene="sufC"
                     /locus_tag="IVO69_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7400099.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17742..18590)
                     /locus_tag="IVO69_001315"
     CDS             complement(17742..18590)
                     /locus_tag="IVO69_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7400100.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18614..19300)
                     /locus_tag="IVO69_001316"
     CDS             complement(18614..19300)
                     /locus_tag="IVO69_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400101.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19293..20327)
                     /locus_tag="IVO69_001317"
     CDS             complement(19293..20327)
                     /locus_tag="IVO69_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7400102.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20722..21060)
                     /locus_tag="IVO69_001318"
     CDS             complement(20722..21060)
                     /locus_tag="IVO69_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7400103.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21062..21418)
                     /locus_tag="IVO69_001319"
     CDS             complement(21062..21418)
                     /locus_tag="IVO69_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7400104.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21424..22611)
                     /locus_tag="IVO69_001320"
     CDS             complement(21424..22611)
                     /locus_tag="IVO69_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7400105.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22802..23647)
                     /locus_tag="IVO69_001321"
     CDS             complement(22802..23647)
                     /locus_tag="IVO69_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7400106.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23881..24846
                     /locus_tag="IVO69_001322"
     CDS             23881..24846
                     /locus_tag="IVO69_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7400107.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7315 a         5252 c         4238 g         8186 t
ORIGIN      
        1 ttagcatacc cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat
       61 aaactacgcg aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt
      121 ttttatattt ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat
      181 atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa
      241 aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa
      301 tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa
      361 aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac
      421 aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa
      481 atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc
      541 cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga
      601 tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat
      661 cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg
      721 tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg
      781 tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg
      841 tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag
      901 tgtgcatagt taaaattccc acgcttttac taataaattc actataacca gttctttcaa
      961 tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca
     1021 ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga
     1081 atacagcttt actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc
     1141 caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag
     1201 aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag
     1261 cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt
     1321 gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct
     1381 tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact
     1441 gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata
     1501 aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt
     1561 tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg
     1621 gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca
     1681 gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag
     1741 ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa
     1801 acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat
     1861 agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg
     1921 caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca
     1981 tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct
     2041 ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct
     2101 aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc
     2161 tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta
     2221 ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct
     2281 tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa
     2341 catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa
     2401 ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat
     2461 tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta
     2521 gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg
     2581 cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat
     2641 ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa
     2701 aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc
     2761 cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta
     2821 agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg
     2881 aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca
     2941 actgatgata caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa
     3001 agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa
     3061 ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag
     3121 taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc
     3181 cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc
     3241 cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt
     3301 tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc
     3361 ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt
     3421 caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat
     3481 cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct
     3541 ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc
     3601 aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg
     3661 ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg
     3721 gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag
     3781 ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct
     3841 ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca
     3901 ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta
     3961 gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca
     4021 acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca
     4081 attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga
     4141 acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca
     4201 aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc
     4261 atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta
     4321 attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta
     4381 gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct
     4441 ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt
     4501 gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt
     4561 aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac
     4621 gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc
     4681 ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag
     4741 gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat
     4801 accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga
     4861 tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct
     4921 gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa
     4981 atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg
     5041 gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg
     5101 acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt
     5161 gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc
     5221 acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct
     5281 ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt
     5341 aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg
     5401 taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat
     5461 caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac
     5521 gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa
     5581 ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg
     5641 gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa
     5701 accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat
     5761 aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta
     5821 aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg
     5881 gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga
     5941 atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta
     6001 atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc
     6061 ataataattt tttctttttc atttctttta tattgttata acagtgaata acataaaaat
     6121 tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac
     6181 tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa
     6241 tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt
     6301 ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct aatatagctt
     6361 tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat
     6421 ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc
     6481 cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta
     6541 taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa
     6601 taatttgctc ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg
     6661 ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata
     6721 agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta
     6781 ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt
     6841 gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg
     6901 tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt
     6961 ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta
     7021 gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt acacttaata
     7081 tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac
     7141 ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt
     7201 cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta
     7261 tatacaacaa tatgatgtat ttcaactcat aaaatacaaa aaacttcaaa taccattaca
     7321 aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata
     7381 tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga
     7441 aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa
     7501 cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga
     7561 caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga
     7621 agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa
     7681 tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga
     7741 gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc
     7801 tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta
     7861 tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta
     7921 aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag
     7981 ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt
     8041 tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct
     8101 ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta
     8161 aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag
     8221 cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat
     8281 gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat
     8341 taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct
     8401 cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt
     8461 tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat
     8521 aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag
     8581 tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac
     8641 gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa
     8701 gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt
     8761 ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca
     8821 tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg
     8881 aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac
     8941 atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat
     9001 aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca
     9061 tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat
     9121 actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa
     9181 gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc
     9241 ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt
     9301 tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa
     9361 agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg
     9421 cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat
     9481 ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg acatccagaa
     9541 taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg
     9601 gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat
     9661 agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta
     9721 ataatttttc ttgatctaca taaattggaa tattaatcat taatgtttcc atttgtgttt
     9781 cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat
     9841 tactatatgt tttagtctta tttgtttact caacctttat accgttccat gaaactatca
     9901 atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga
     9961 gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag
    10021 tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta
    10081 attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga
    10141 gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta
    10201 ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta
    10261 ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca tctttgtaaa
    10321 atttatttac aacacccctt tttttgaggt atactaattt taggaggtgt ggtcaatatg
    10381 ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa
    10441 gctataagaa agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt
    10501 caattaattg acaaaaaaac tatctacgaa tgggagaagg gaacctattt accaatacca
    10561 gagcgattat cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc
    10621 aatgtagaag aatatatttt gggaattatt ctttatagag atagcattgt actagatggt
    10681 ataacatttc cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac
    10741 tctaatttag atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt
    10801 attgctaaca agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta
    10861 aaaatagaag aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc
    10921 ctcactagca gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa
    10981 ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag
    11041 ctagaacaaa cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt
    11101 gataccatct agtggtaagt actccttgta aactaagagg agatgattta atggcaaaaa
    11161 tatcaaagta caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca
    11221 ccgatccacg aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag
    11281 aagctcaaaa agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag
    11341 cacctgatat gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg
    11401 ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat
    11461 ttggtaagct taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat
    11521 ggcacagtaa atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct
    11581 acggggttgc aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa
    11641 aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg
    11701 atgcttttaa agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca
    11761 caggtatgag aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata
    11821 aagaactaca tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat
    11881 ctccgaaaac aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc
    11941 tcaacaactg gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct
    12001 ctaaagagca acatgttttt acaacagtta aaaacactct atacattcca aacactgtta
    12061 atgattggtt aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt
    12121 ttagacatac acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac
    12181 aacgattagg gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata
    12241 atgcacctaa gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct
    12301 ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata
    12361 tcaagggttc tagctcctat aacaggttta cccaacactg ccttccatct cataactgat
    12421 caatcgattg agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa
    12481 gcccatgacg atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta
    12541 gagttgttct tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt
    12601 gtggatctca ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc
    12661 acattcgata tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata
    12721 gtttacttct ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg
    12781 tgcattgtgg atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat
    12841 ggagagcatg gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt
    12901 tgtttttgca cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc
    12961 tcgtttggtt actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca
    13021 atacgcatct tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata
    13081 agtaggtgcc gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag
    13141 cgtacgttcg aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac
    13201 atcgactttg acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt
    13261 cagcgctgct aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa
    13321 ttcagggtat tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa
    13381 ttcatctttc atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag
    13441 ataagctcgt tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg
    13501 tacgtcttcc cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc
    13561 gaaatcgatg tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc
    13621 tagagatttc agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc
    13681 acggatcact tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg
    13741 aaacccgaat tcatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt
    13801 ggtaatgatg gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg
    13861 caagtgtcgc acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat
    13921 cgcctaattg gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag
    13981 ttaaatgttc tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac
    14041 ttgccgtact gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt
    14101 ttacatcgac ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc
    14161 ttgtcgagtc agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt
    14221 gacggtataa attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg
    14281 attgcttcaa ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga
    14341 gcagtcgctg atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg
    14401 acggctaccc cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa
    14461 ttgaatgcaa cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca
    14521 tcaattgctt gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata
    14581 ttgtccatac cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg
    14641 atatttggtg tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat
    14701 tcgacaaaat cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt
    14761 cgtttaccat ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca
    14821 aagaaatcac agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca
    14881 tctactacga tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg
    14941 aagacaccta agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc
    15001 tttttcgctg catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag
    15061 acagcacctg ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg
    15121 taagagagca ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc
    15181 caattcaagc ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg
    15241 atgaacgccc gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac
    15301 gtatggacac cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg
    15361 agtacttgtt ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca
    15421 ttgacgtctt gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat
    15481 tcaacttccc ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg
    15541 ttaagacaga accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc
    15601 ctctactcat cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc
    15661 cagcagttac ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat
    15721 cagaaagcat caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga
    15781 tgtggccgat accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa
    15841 tatgcccgat cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc
    15901 tgcccgagct gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg
    15961 agtcaaagtc agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag
    16021 catctcgtaa aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat
    16081 ctacggctga atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac
    16141 ctgatgattt tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac
    16201 tatgttcatc tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt
    16261 gaatgaataa ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa
    16321 taccgctgtt cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg
    16381 cttttttcat ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt
    16441 cggtaaaaat gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg
    16501 aaccttgttg gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg
    16561 tttcactgat ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc
    16621 gatcgatatg cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc
    16681 tcatccaatc aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga
    16741 gccagttttt ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc
    16801 ctaattcttt actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat
    16861 tccctgtcat gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt
    16921 tcaataatcg ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat
    16981 ttactccttt agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta
    17041 aaattgcaaa tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc
    17101 cgccagaaaa accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag
    17161 ccattttttc atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac
    17221 gtttcgcatt gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg
    17281 gatattgcat agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa
    17341 catttttccc gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga
    17401 tagcggcaga taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc
    17461 cggttttcat tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta
    17521 gattttttat ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc
    17581 attttagact aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt
    17641 atttattatt aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata
    17701 agccattaca gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt
    17761 actggtttaa cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga
    17821 agcacttcaa tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata
    17881 atattcacgt aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac
    17941 cctgcgccgt aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac
    18001 gtagaagcta aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct
    18061 tcaatatcat cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca
    18121 gcatcttgta gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt
    18181 gttgcaccat cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt
    18241 tcgatgtgga cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc
    18301 aaataaggaa tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc
    18361 ggtaatacat agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat
    18421 ggtttcacat gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc
    18481 gtgtcgtctg ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat
    18541 cctacagtca gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc
    18601 cccctatatc ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa
    18661 cgataaatac gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac
    18721 gctggtagcc ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca
    18781 tcgcagtaaa tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa
    18841 tactttcagg tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg
    18901 ctgcttccag tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa
    18961 atggtgctgc ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta
    19021 cgattgcttt agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga
    19081 aaatattact gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc
    19141 gtccaataga gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg
    19201 agattaatgt catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt
    19261 gtttaaaatc tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct
    19321 actccttgtt ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc
    19381 aagacagtca atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg
    19441 ttgacatcga tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt
    19501 tcgctaaagg tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat
    19561 agttctgtcg aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct
    19621 tttttcggtt gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc
    19681 atcacagcga ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg
    19741 atggtcaaat tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt
    19801 tgcgggtcca atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat
    19861 gctcgtgcga taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt
    19921 ccgcgtcctt ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc
    19981 tttggtacgc cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat
    20041 aacaggttga aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg
    20101 ttttttaatt ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca
    20161 agtccattca ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca
    20221 taaatatccc ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt
    20281 ttcccattga atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct
    20341 ttctcttata atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga
    20401 aagcttgaac ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc
    20461 cagtacttgg acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt
    20521 aagtttgctt acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc
    20581 cattttcagc aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc
    20641 tttctcgttt catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt
    20701 ttttcaagtg attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa
    20761 acttaagatc cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat
    20821 tttatcatta acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt
    20881 ttccgcttca acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag
    20941 attggcaaac gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta
    21001 ttcttttcca ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat
    21061 tttatatcag ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct
    21121 ctcgtaagat cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt
    21181 ttgaagccgt aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc
    21241 ttgtgttgaa caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg
    21301 aggccttcgg tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta
    21361 accacgcttt ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt
    21421 ttcttacctc cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa
    21481 cccgataccg atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg
    21541 gatccctgtt aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag
    21601 cagcatcatg atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca
    21661 cacccgaatc atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat
    21721 aaagccgaag ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata
    21781 gatattgctt acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc
    21841 ttgggccggc tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat
    21901 acgtgcaaac tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt
    21961 cgtaaccaag aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat
    22021 gatgcgccaa gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag
    22081 catcgttcca aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa
    22141 gagcttccca atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg
    22201 tttagttacg atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg
    22261 gaaagtaaac gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg
    22321 atcatagaat ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt
    22381 cagtttccag atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac
    22441 taaataccag acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg
    22501 gctaagtgct acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat
    22561 cacgccataa tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc
    22621 cttttcttaa aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct
    22681 ttccatagac atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag
    22741 tacaaaagcc tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta
    22801 tctaccagct gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga
    22861 tctcttcggc aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa
    22921 aatatcgatg gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca
    22981 aaatcgagcg accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga
    23041 tcatcttctt atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac
    23101 cagagtttgc ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct
    23161 ggtcttccat caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac
    23221 caaaatcgga ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga
    23281 tctcaaaatg cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat
    23341 agacagcaaa ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga
    23401 gcaatttttg ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata
    23461 agtcggcttg cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat
    23521 tcaattcccg ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta
    23581 cttctgctgc tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact
    23641 taaacatggc accacttcca taacttttta tgataattac tataaaaact atttgattta
    23701 aatatacaaa aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag
    23761 taacttagat gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt
    23821 tacaatgtat tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat
    23881 atgaagaact ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct
    23941 tcccttggaa atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt
    24001 atctctatga ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg
    24061 aaacaaacgt tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc
    24121 atggttattt gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt
    24181 tggctgtgcg ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt
    24241 atcaaagctg atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga
    24301 tttggggtca tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt
    24361 tggatttcat tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc
    24421 gtaagaaata tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct
    24481 ctttgcctaa caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca
    24541 ttgttcgctt tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc
    24601 aagcttcgtt tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca
    24661 gctatattta ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat
    24721 ttcttaaaag ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat
    24781 tacgtacgtt tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc
    24841 gcttaataat cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg
    24901 ccaaacaaaa acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag
    24961 cttttgaaca ccgtttacca agaggtcgta a
//
LOCUS       DACCPY010000038        24914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCPY010000038 DACCPY010000000
VERSION     DACCPY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.038"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(88..312)
                     /locus_tag="IVO69_001323"
     CDS             complement(88..312)
                     /locus_tag="IVO69_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7400108.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(510..2090)
                     /locus_tag="IVO69_001324"
     CDS             complement(510..2090)
                     /locus_tag="IVO69_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7400109.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2491..3867)
                     /locus_tag="IVO69_001325"
     CDS             complement(2491..3867)
                     /locus_tag="IVO69_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7400110.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3995..5113)
                     /locus_tag="IVO69_001326"
     CDS             complement(3995..5113)
                     /locus_tag="IVO69_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7400111.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5255..5521
                     /locus_tag="IVO69_001327"
     CDS             5255..5521
                     /locus_tag="IVO69_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400112.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGMPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILLKMLACLIFI"
     gene            complement(5613..6146)
                     /locus_tag="IVO69_001328"
     CDS             complement(5613..6146)
                     /locus_tag="IVO69_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7400113.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="IVO69_001329"
     CDS             complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="IVO69_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7400114.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7411..8208)
                     /gene="recX"
                     /locus_tag="IVO69_001330"
     CDS             complement(7411..8208)
                     /gene="recX"
                     /locus_tag="IVO69_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7400115.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8315..9697
                     /gene="rlmD"
                     /locus_tag="IVO69_001331"
     CDS             8315..9697
                     /gene="rlmD"
                     /locus_tag="IVO69_001331"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7400116.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9871..10449)
                     /locus_tag="IVO69_001332"
     CDS             complement(9871..10449)
                     /locus_tag="IVO69_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7400117.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10771..11241
                     /locus_tag="IVO69_001333"
     CDS             10771..11241
                     /locus_tag="IVO69_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7400118.1"
                     /translation="MKMIKDIRFWVCGIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11349..12575)
                     /locus_tag="IVO69_001334"
     CDS             complement(11349..12575)
                     /locus_tag="IVO69_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7400119.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYIPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13329..13529)
                     /locus_tag="IVO69_001335"
     CDS             complement(13329..13529)
                     /locus_tag="IVO69_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7400120.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13663..14271)
                     /locus_tag="IVO69_001336"
     CDS             complement(13663..14271)
                     /locus_tag="IVO69_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7400121.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14356..14826)
                     /locus_tag="IVO69_001337"
     CDS             complement(14356..14826)
                     /locus_tag="IVO69_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7400122.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14874..15788)
                     /locus_tag="IVO69_001338"
     CDS             complement(14874..15788)
                     /locus_tag="IVO69_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7400123.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15934..16737
                     /locus_tag="IVO69_001339"
     CDS             15934..16737
                     /locus_tag="IVO69_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7400124.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16915..17862
                     /locus_tag="IVO69_001340"
     CDS             16915..17862
                     /locus_tag="IVO69_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7400125.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17930..18223)
                     /locus_tag="IVO69_001341"
     CDS             complement(17930..18223)
                     /locus_tag="IVO69_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7400126.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18251..18916)
                     /locus_tag="IVO69_001342"
     CDS             complement(18251..18916)
                     /locus_tag="IVO69_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7400127.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19056..19688)
                     /locus_tag="IVO69_001343"
     CDS             complement(19056..19688)
                     /locus_tag="IVO69_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400128.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19695..20762)
                     /locus_tag="IVO69_001344"
     CDS             complement(19695..20762)
                     /locus_tag="IVO69_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7400129.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(20759..21490)
                     /locus_tag="IVO69_001345"
     CDS             complement(20759..21490)
                     /locus_tag="IVO69_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7400130.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21658..22137)
                     /gene="greA"
                     /locus_tag="IVO69_001346"
     CDS             complement(21658..22137)
                     /gene="greA"
                     /locus_tag="IVO69_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7400131.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22273..23448)
                     /gene="mltG"
                     /locus_tag="IVO69_001347"
     CDS             complement(22273..23448)
                     /gene="mltG"
                     /locus_tag="IVO69_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7400132.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23613..24773
                     /locus_tag="IVO69_001348"
     CDS             23613..24773
                     /locus_tag="IVO69_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7400133.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         7437 a         5063 c         4000 g         8414 t
ORIGIN      
        1 aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa
       61 cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac
      121 ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg
      181 gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa
      241 taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtatttgcca
      301 ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta
      361 tatttttttg attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa
      421 tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt
      481 gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattagga
      541 tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa tggttggtcg
      601 aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga
      661 tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca
      721 tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat
      781 tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt
      841 cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct
      901 ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc
      961 ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg
     1021 acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt
     1081 gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa
     1141 gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa
     1201 aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct
     1261 ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa
     1321 ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca
     1381 gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga
     1441 ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa
     1501 ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct
     1561 ttacccatac cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat
     1621 tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat
     1681 atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct
     1741 tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct
     1801 tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca
     1861 aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct
     1921 gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca
     1981 ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc
     2041 gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat aacctaactc
     2101 catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag
     2161 aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa
     2221 acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc
     2281 tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca
     2341 actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca ccaaaccata
     2401 agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa
     2461 gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc
     2521 ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa
     2581 aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt
     2641 atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa
     2701 ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa
     2761 ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag
     2821 attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc
     2881 tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg
     2941 cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg
     3001 ctcctgcatg attttcttga tatctaaatt atcaaaaccg aatttatggg cagcttttag
     3061 taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat
     3121 tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc
     3181 aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga
     3241 aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat
     3301 ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat
     3361 acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc
     3421 aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat
     3481 acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat
     3541 gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga
     3601 taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat
     3661 tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc
     3721 tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga
     3781 agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag
     3841 cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta
     3901 gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt
     3961 ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct
     4021 tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc
     4081 agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta
     4141 ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca attacattag
     4201 gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg
     4261 cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc
     4321 ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca
     4381 caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt
     4441 gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt
     4501 ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca
     4561 acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc
     4621 caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt
     4681 aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt
     4741 cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca
     4801 atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg
     4861 caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca
     4921 acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa
     4981 ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca
     5041 tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa
     5101 gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat attcgttaaa
     5161 aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt
     5221 ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac
     5281 aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca
     5341 gggtatgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct
     5401 gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa
     5461 aatgaaatta cagcggatgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
     5521 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
     5581 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
     5641 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
     5701 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcggata attcatcatt
     5761 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
     5821 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
     5881 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
     5941 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
     6001 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
     6061 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
     6121 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
     6181 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
     6241 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
     6301 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
     6361 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
     6421 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
     6481 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
     6541 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
     6601 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
     6661 gctccagaat gattttgaag agccgcactg atataataaa cgtcttttgg ctttgctttt
     6721 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
     6781 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
     6841 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
     6901 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
     6961 actcgcatca cgttgccgtc aaccgcgggt tctggaagtc caaatgcgat acttgcaatc
     7021 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
     7081 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
     7141 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
     7201 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
     7261 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
     7321 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
     7381 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
     7441 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
     7501 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
     7561 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcca tcgcttgttg
     7621 gatcacttct tgagaaaaac ctttcgtcat caatgtttgt tggatttttc tcaataattc
     7681 tttttggctt tttccgcgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
     7741 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
     7801 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
     7861 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
     7921 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
     7981 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
     8041 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
     8101 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
     8161 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
     8221 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
     8281 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
     8341 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
     8401 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
     8461 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
     8521 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
     8581 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
     8641 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
     8701 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
     8761 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
     8821 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
     8881 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacgatca tggttagaat
     8941 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
     9001 aaaaaacgca atgatctgtg agatcaatga acgactgccc gaagttgttt caatcatgca
     9061 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
     9121 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
     9181 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
     9241 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
     9301 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
     9361 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
     9421 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
     9481 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
     9541 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
     9601 taaaaagttc aagattgaat atttacagtc tgtcgacatg tttcctcaga ctgcacgttg
     9661 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
     9721 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
     9781 ccgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
     9841 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
     9901 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
     9961 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    10021 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    10081 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    10141 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    10201 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    10261 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    10321 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    10381 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    10441 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    10501 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    10561 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga accaaatcat
    10621 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    10681 tcatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    10741 aaactactat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    10801 gtttgtggta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    10861 ggagaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    10921 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    10981 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    11041 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    11101 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctaca tcatcgcctg
    11161 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    11221 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    11281 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    11341 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    11401 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    11461 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    11521 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    11581 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    11641 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    11701 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    11761 aactggtctt gtttgattgt ttcaaacgca gggatgtagt tcgtcgctac atcaagcatg
    11821 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    11881 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    11941 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    12001 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    12061 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    12121 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    12181 gccatttcga caccaccaaa tgcataggca tgatttaatt tatctaccgt tccataacct
    12241 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    12301 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    12361 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    12421 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    12481 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac aaatacccct
    12541 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    12601 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    12661 ttaaaaaaca tttttgtgta acgattacta ttttttaaaa tttctcaaaa ttcttaacta
    12721 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    12781 ctttttttac gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    12841 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    12901 ttatcattcg atttcacgaa aatttcttcc tatgactggt ccttttgtta aacccatctt
    12961 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgatttc
    13021 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    13081 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    13141 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    13201 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    13261 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    13321 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    13381 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    13441 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    13501 ttgaaccatt taaccgttcc gtgttccata aatgtatagc ctccaaaaaa gttcttgcaa
    13561 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    13621 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    13681 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    13741 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    13801 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    13861 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    13921 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    13981 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    14041 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    14101 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg catttaaatt
    14161 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    14221 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    14281 cctcctcaat gttttcttta taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    14341 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    14401 agtttcttta aaaataaaca acttgctgaa aatatagttc gctaggataa caataatttg
    14461 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    14521 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    14581 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    14641 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    14701 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    14761 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    14821 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    14881 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    14941 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    15001 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    15061 ttttcgctat actgccaaat atggacatga tgaacattct ttaccccatc gattgcttca
    15121 atttcttgtt ccattttttc atagtcaata gcaggtgcac tttgtatcaa aatagcggca
    15181 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    15241 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    15301 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    15361 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    15421 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    15481 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    15541 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    15601 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    15661 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    15721 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    15781 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    15841 accaatcatt cagatgtttc cctaaagcac ttgtatttca cttgatactt cgctagaatt
    15901 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    15961 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    16021 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    16081 gtcttaaata acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    16141 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    16201 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    16261 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    16321 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    16381 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    16441 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    16501 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    16561 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    16621 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    16681 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    16741 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    16801 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    16861 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    16921 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    16981 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    17041 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    17101 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    17161 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    17221 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    17281 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    17341 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    17401 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    17461 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    17521 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    17581 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    17641 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    17701 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    17761 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    17821 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    17881 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    17941 taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt
    18001 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatccatt tccactttct
    18061 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    18121 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    18181 agtcaaacca tttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    18241 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    18301 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    18361 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    18421 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    18481 tatctgacag ctctaagtag tctagaatat tctttgatac taagttatgt gcaatccgta
    18541 tccccatgtc tcgctcaggg tgtacgacac gatctgctcc gatcttctct agtacacggg
    18601 catggtactc attttgggct tttgctaaaa tattcggtac gcccatttcc tttaccatca
    18661 gagtaactaa gatgctggct tggatatctt ctccaatagc gacaattaca tgatcgaaat
    18721 ttcttatacc taatgaacga agtgtcatct catcctgtgc atttgccaca acagcatggg
    18781 tggctatatt catatattca ttgactcgat cttcattact gtcaatagcc agtacttctt
    18841 gtccagcttc gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg cctacaattg
    18901 caaaattttg tttcaccatt ttcctccttt agtatcttct tttctcagta taacaaagcc
    18961 tttccaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    19021 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttaaac
    19081 gcatagatag ctgcttgagt tcggtcttct acctctaact tagctaatat attcgacaca
    19141 tgcgttttga ctgttttcag tgtgataaac aattcatccg cgatttcctg attacttttc
    19201 ccttcagaaa tcagcatcaa aatttcattt tctcgattcg ttaattcttc atgcaatact
    19261 ggatcatttc gccgactcat tttttccatc atttttgttg tcacttcagg ttctagtact
    19321 cgctcgccgc gttgcgtagc ccttatagca tcagctattt catgtgctgt agacgtcttc
    19381 aacaaatatc ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc atcaataaaa
    19441 cttgtgacga taatgatttt ggcttttggc caatctttta agattgcttt tgttgattcg
    19501 atgccatcca tttcttccat gactagatcc attaaaatga catccggtcg aagtgccatc
    19561 gccttttcat agccttgacg tccattttca gcttctccaa tcacttcaat gtcttcttgg
    19621 atagataaat aagaagagac gcctaagcgt accatttcat ggtcatctac taataaaact
    19681 tttatcatct ttatctatgc ctccttcatc aaagggacct tggtctccac gctcgtacct
    19741 tgccctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    19801 ttgttcaatc cataacttcc cgttttggtt tcattcatat taaatcctgt cccgtcgtca
    19861 atgatacgta ataaaagatt gttatctatt ttatgcaaat agacttctaa ttcattagcc
    19921 ttagcatgtc ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    19981 atggaactag ttaattttac atcttctacc tcccatttta gcgaaatctg gattttattc
    20041 tgcaattcct tcaataactg ctcaatgcct tgttttaaac ttttttcttc taaattgact
    20101 ggacgcagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgcc
    20161 accattttca actgcttacg gaacatttct ggtgtctcac ttttttctgc ttgctccgtc
    20221 aatgccgaca tcatcatcat cgctgcaaat aattgctgtg aaactgaatc atgaagctca
    20281 cgagctaatc gatgcctttc taattttaaa atctcttctt tggtttgtcc atctacatat
    20341 tgaggacggc tagtcataat ctgtaattca ctggatactt cgatcatttt ttcttttatt
    20401 ttagtaattt ccatatcaat atctttgatg tacagatcat ccgacgcgct tgggatcggc
    20461 tgatgcaaaa gtttggattc ataattgcca gaagaaagtg cacgaagctt ttcttcgatt
    20521 ttcccgtatt ttgccttttg aataagtgat aacaatagaa atacgatcaa tcctacacct
    20581 aatgaaattg ccatcaaatg gaagataagc ggaacgtata ataaacgcgc acgcagcaat
    20641 tctccccacc aatgactttg gttactggca taaaaataag tgaaaagtga aaatagaata
    20701 ataaggaaag ccgcaatccc cgaataaaca gctagcattg ctttggatat tcttcccatc
    20761 atattcgtat cacctcgaca tctccaacca aagtattggt aatgatcttc aatctgcgag
    20821 gattttcatc ataatcactg ctgcagattt tgatctgttc gttttttaag gaaaactgct
    20881 cttcttcaaa caagacattt cctactaatg tcgcatgctc taaacgaata gccactccca
    20941 ggggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    21001 ttggcaaaag tgtattgccc aaatcgatga tagtatcacc agaaatgatg gcaatgttga
    21061 tatcatccca ttcatagacg tcgtttccaa tacgttggtt accaaataat tgctgtcttt
    21121 tacgctcatt attatgtgtt tttaattgct ctgtttggac catcatgatc tgtttttttc
    21181 gccaaaaagc attttttgtc aaatctattc ctgaaatctc tacacctttc agcccgataa
    21241 ataaaacggc caatacaacc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    21301 tgaccaagct ccctaagatc aattgaaaat tttgaaattt agtacgagga tatttgcgca
    21361 tagctagata tatattgaaa ataccgaata ttactaaaag caaaagtcca gtattgttta
    21421 cgatttgcca aacagcaaat aacagcagca gtgcttcgac taccacaaaa aaacgccaag
    21481 aactattcat cgctttttct ccctcctgac acattctcat ttcatcatac ctattttttt
    21541 gactgttggc tataggactt tagatgtaag atcgttcagt gaaacctgca atactcaaag
    21601 agaaaagatc tagagtgcgt catcagtaag atgaactctc tagatctttc agtcagacta
    21661 cgcttgttct actttgacga ttttaactaa catatcgcca cctggtgtag caatggttac
    21721 ttcatcattt ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat tggaaatctt
    21781 tccagaaaac gggtctgctt cagcacttcc gacaatcgtg tactcttctt catctccatc
    21841 aggcaactcg atgaacgtaa ctgttttacc gatcgatacc tcatctgcat ctacgccgtc
    21901 attatcgatg atttgtgcaa aacggatcat attttcaatt gttgtgattc ttccttcaac
    21961 aaatgcttgt tcatctttag ctgattcgta ttcggagttt tcggaaagat ctccgaaacc
    22021 gcgagcgatt ttgattcgtt caatgatttc tccgcgttta actgttttta gttcttctaa
    22081 ttctttttct aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg
    22141 taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca
    22201 aaaaagatta ccacgctttt cttaaaatgt aaatgttttt ctaaaaatga tcaatgacta
    22261 gcccctgttt ttctaagaat tatttacata tttttgcact agttccatat gttcttcata
    22321 cgttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt
    22381 ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggaccagg
    22441 cccatagccg gtattggtat acaaattgta tggtgaatct acagctgtat cttcatacgt
    22501 caccaattct ttatgttctc ctaatgcata taaaacagag atatctgact gaagtggcat
    22561 atttgccttg attcggttaa agaagacttg tgcaatattt ttacgatcat tttcttttac
    22621 gccttctttt tcaactagag aagccaaagt taaaacttct tgtacagtca aatttttctg
    22681 tttgatagct gaataatagt tttgcatgac cgtatttgtt ttgttcacca tctcaatcac
    22741 aagatcctta agcgaagtac tcgtgtagta atcataagta gctgggaata agtagccttc
    22801 caaacggtat tttgtgttct cagctttaga ggcactttcc aacaattctg ggaatttttg
    22861 atacaactcg ttaaagaaag tctcatcatt tactaaatct aaaaattctt tcttgtcttt
    22921 accagttacc ttcgctaccc gctcagcaat ctgatcgatg tcgtagcctt ctggaatggc
    22981 aattttccca tctgcaactt ttgttggctc agaagtgccg ccttcttgca gctgtttgcc
    23041 aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa
    23101 atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc
    23161 caaaatctca ccaatctgct tgttagaaga gccactcggg atctccactt ggactaattg
    23221 gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc
    23281 aagaaatccg ccaataatta ataatgtaag agcaatcact aaaatgatct tcctcacaat
    23341 cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt
    23401 actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc
    23461 ttactatcat cttcgtaact ctgtttatta tacatgaaat ttctactgcc gtgaattatt
    23521 ttaccaaata ttatgtcaaa attgtaagtg gcaaatactc tttttgagga ttacaatgaa
    23581 aaatagttaa aaatggatag aaaggaatta acatgtcaaa aggaaaacat cgaaaaaaac
    23641 aaaaaatttc tatttctttt cttgtaattt tattattgtc tgtcagcttc ttttcaacag
    23701 gcatcattat aggaggtcaa ttaacaaaaa tacctcattc ccaaacttat aaagcggagc
    23761 caacacaact tacttccaat ttaacgcccg atgagcagag gcttgctagc aaaattgatc
    23821 aattactaca aaaatcagat tatatcggtt cgatttatgt tcgcaaaaat aatcgcgtac
    23881 tattagaaaa aggatatgga tatgcaaatc tgcgcgtaaa agaatcgaat tcaccttcta
    23941 tttactatca aatcggttcg attcaaaaag caatgactgc tttactgata ctaaaacaaa
    24001 tccaagctgg taagttaact tttgacacaa agttagcaga attttatcct cagattcctg
    24061 gaagtcagca aatcacaatc aaaaatatgc tttatatgcg ctctggtctt catcgaactg
    24121 cttctcccga caaacctatg tcggatagtg aaatcattca gttcgccctt catcatttga
    24181 aacttactga ttatgatacg tatcattatg aaccattgaa ttatacattg cttacaggga
    24241 tattgatcaa actaacgaat cagccttatg aaactcttct aaaaaaagaa atcattcgtc
    24301 cactccatct agaacataca gatttttatg acaatgtaaa aaattcgcct caacatgctg
    24361 tatcttacca gatgtctgca actgacgatt attcaaaagc attgatcgag cctgaaaccg
    24421 acatccgaaa tgaattaggt acaggaaaca taagtatgtc tgtttatgac ttgaacaaat
    24481 tctttaccag tgttctttct ggcggtatca taccaaaaga actacttttc tcattgtggc
    24541 aaaataatgg tgataaacat ccttacagcg gcggtgttta cagtggaaac gattatatcc
    24601 ttgctcaggg gaatatcaat cgattccatg cagtagtggc gatgaaaaaa gacactaaag
    24661 atgcaatcgt catggaaagt aatatccaat cggataagaa catcaaattg ccggccactg
    24721 atttaagaaa tcagatttat gaactgatgg aaggtgttca cttatcaaat taaattttga
    24781 cataatatta gttgtcatct ctgtatagtc aatttagtta gtactctatc tgaaaagtag
    24841 aaataaatta acaaatatta tctgtcctta ttatttgtta atttatttct tattttactt
    24901 aaataatttt tggt
//
LOCUS       DACCPY010000039        24818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCPY010000039 DACCPY010000000
VERSION     DACCPY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.039"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..213)
                     /locus_tag="IVO69_001349"
     CDS             complement(<1..213)
                     /locus_tag="IVO69_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400134.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIE"
     gene            complement(398..796)
                     /locus_tag="IVO69_001350"
     CDS             complement(398..796)
                     /locus_tag="IVO69_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7400135.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(793..1566)
                     /locus_tag="IVO69_001351"
     CDS             complement(793..1566)
                     /locus_tag="IVO69_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7400136.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1563..2246)
                     /locus_tag="IVO69_001352"
     CDS             complement(1563..2246)
                     /locus_tag="IVO69_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7400137.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2413..3300)
                     /locus_tag="IVO69_001353"
     CDS             complement(2413..3300)
                     /locus_tag="IVO69_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7400138.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3297..4166)
                     /locus_tag="IVO69_001354"
     CDS             complement(3297..4166)
                     /locus_tag="IVO69_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400139.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4303..5589)
                     /locus_tag="IVO69_001355"
     CDS             complement(4303..5589)
                     /locus_tag="IVO69_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7400140.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5862..6440
                     /locus_tag="IVO69_001356"
     CDS             5862..6440
                     /locus_tag="IVO69_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7400141.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6440..7369
                     /locus_tag="IVO69_001357"
     CDS             6440..7369
                     /locus_tag="IVO69_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400142.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7366..9102
                     /locus_tag="IVO69_001358"
     CDS             7366..9102
                     /locus_tag="IVO69_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7400143.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVO69_001359"
     CDS             complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVO69_001359"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7400144.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9998..10507)
                     /locus_tag="IVO69_001360"
     CDS             complement(9998..10507)
                     /locus_tag="IVO69_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7400145.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVO69_001361"
     CDS             complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVO69_001361"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7400146.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11808..12899)
                     /locus_tag="IVO69_001362"
     CDS             complement(11808..12899)
                     /locus_tag="IVO69_001362"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7400147.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVO69_001363"
     CDS             complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVO69_001363"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7400148.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVO69_001364"
     CDS             complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVO69_001364"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7400149.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVO69_001365"
     CDS             complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVO69_001365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7400150.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16862..17836)
                     /locus_tag="IVO69_001366"
     CDS             complement(16862..17836)
                     /locus_tag="IVO69_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7400151.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17817..19700)
                     /locus_tag="IVO69_001367"
     CDS             complement(17817..19700)
                     /locus_tag="IVO69_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7400152.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19718..21943)
                     /locus_tag="IVO69_001368"
     CDS             complement(19718..21943)
                     /locus_tag="IVO69_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7400153.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22058..23092
                     /locus_tag="IVO69_001369"
     CDS             22058..23092
                     /locus_tag="IVO69_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400154.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23159..24172)
                     /locus_tag="IVO69_001370"
     CDS             complement(23159..24172)
                     /locus_tag="IVO69_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7400155.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24250..24438)
                     /locus_tag="IVO69_001371"
     CDS             complement(24250..24438)
                     /locus_tag="IVO69_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7400156.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7139 a         5392 c         4427 g         7860 t
ORIGIN      
        1 ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca aaacagccga
       61 cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga actctggtga
      121 aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag atcgctcaat
      181 cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt catcattttt
      241 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      301 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      361 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      421 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      481 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      541 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      601 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      661 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      721 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      781 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      841 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      901 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      961 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
     1021 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
     1081 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1141 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1201 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1261 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1321 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1381 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1441 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1501 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1561 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1621 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1681 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1741 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1801 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1861 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1921 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1981 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     2041 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     2101 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2161 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2221 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2281 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2341 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2401 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2461 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2521 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2581 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2641 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2701 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2761 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2821 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2881 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2941 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     3001 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     3061 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3121 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3181 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3241 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3301 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3361 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3421 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3481 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3541 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3601 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3661 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3721 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3781 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3841 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3901 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3961 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     4021 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     4081 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4141 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4201 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4261 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4321 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4381 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4441 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4501 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4561 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4621 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4681 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4741 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4801 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4861 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4921 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4981 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     5041 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     5101 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5161 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5221 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5281 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5341 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5401 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5461 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5521 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5581 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5641 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5701 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5761 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5821 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5881 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5941 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     6001 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     6061 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6121 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6181 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6241 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6301 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6361 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6421 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6481 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6541 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6601 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6661 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6721 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6781 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6841 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6901 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6961 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     7021 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     7081 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7141 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7201 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7261 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7321 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7381 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7441 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7501 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7561 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7621 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7681 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7741 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7801 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7861 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7921 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7981 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     8041 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     8101 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8161 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8221 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8281 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8341 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8401 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8461 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8521 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8581 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8641 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8701 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8761 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8821 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8881 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8941 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     9001 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     9061 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9121 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9181 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9241 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9301 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9361 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9421 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9481 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9541 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9601 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9661 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgcgaaag gatttttgta
     9721 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9781 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9841 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9901 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9961 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
    10021 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
    10081 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10141 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10201 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10261 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10321 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10381 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10441 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10501 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10561 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10621 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10681 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10741 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10801 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10861 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10921 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10981 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    11041 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    11101 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11161 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11221 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11281 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11341 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11401 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11461 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11521 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11581 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11641 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11701 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11761 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11821 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11881 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11941 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    12001 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    12061 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12121 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12181 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12241 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12301 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12361 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12421 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12481 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12541 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12601 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12661 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12721 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12781 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12841 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12901 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12961 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    13021 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    13081 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13141 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13201 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13261 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13321 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13381 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13441 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13501 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13561 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13621 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13681 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13741 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13801 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13861 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13921 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13981 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    14041 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    14101 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14161 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14221 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14281 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14341 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14401 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14461 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14521 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14581 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14641 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14701 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14761 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14821 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14881 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14941 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    15001 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    15061 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15121 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15181 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15241 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15301 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15361 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15421 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15481 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15541 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15601 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15661 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15721 cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa
    15781 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15841 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15901 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15961 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    16021 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    16081 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16141 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16201 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16261 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16321 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16381 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16441 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16501 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16561 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16621 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16681 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16741 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16801 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16861 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16921 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16981 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    17041 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    17101 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17161 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17221 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17281 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17341 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17401 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17461 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17521 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17581 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17641 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17701 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17761 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17821 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17881 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17941 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    18001 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    18061 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18121 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18181 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18241 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18301 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18361 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18421 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18481 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18541 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18601 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18661 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18721 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18781 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18841 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18901 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18961 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    19021 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    19081 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19141 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19201 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19261 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19321 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19381 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19441 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
    19501 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19561 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19621 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19681 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19741 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19801 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19861 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19921 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19981 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    20041 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
    20101 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20161 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20221 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20281 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20341 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20401 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20461 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20521 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20581 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20641 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20701 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20761 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20821 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20881 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20941 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    21001 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    21061 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21121 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21181 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21241 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21301 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21361 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21421 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21481 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21541 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21601 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21661 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21721 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21781 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21841 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21901 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21961 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
    22021 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
    22081 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
    22141 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
    22201 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
    22261 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
    22321 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
    22381 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
    22441 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
    22501 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
    22561 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
    22621 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
    22681 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
    22741 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
    22801 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
    22861 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
    22921 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
    22981 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
    23041 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
    23101 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
    23161 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
    23221 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
    23281 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
    23341 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
    23401 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
    23461 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
    23521 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
    23581 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
    23641 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
    23701 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
    23761 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
    23821 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
    23881 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
    23941 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
    24001 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
    24061 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
    24121 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
    24181 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
    24241 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
    24301 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
    24361 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
    24421 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
    24481 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
    24541 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
    24601 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
    24661 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
    24721 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
    24781 agtccttatt ggcaaatcgc caaaataatg tagactaa
//
LOCUS       DACCPY010000040        24619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCPY010000040 DACCPY010000000
VERSION     DACCPY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.040"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IVO69_001372"
     CDS             647..964
                     /locus_tag="IVO69_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7400157.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IVO69_001373"
     CDS             complement(1055..1420)
                     /locus_tag="IVO69_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400158.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IVO69_001374"
     CDS             1628..2110
                     /locus_tag="IVO69_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400159.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IVO69_001375"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IVO69_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7400160.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IVO69_001376"
     CDS             complement(3484..4758)
                     /locus_tag="IVO69_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7400161.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IVO69_001377"
     CDS             5031..7502
                     /locus_tag="IVO69_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7400162.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IVO69_001378"
     CDS             complement(7605..9341)
                     /locus_tag="IVO69_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7400163.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IVO69_001379"
     CDS             complement(9338..10042)
                     /locus_tag="IVO69_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400164.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IVO69_001380"
     CDS             10173..10676
                     /locus_tag="IVO69_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7400165.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IVO69_001381"
     CDS             10636..10974
                     /locus_tag="IVO69_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7400166.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IVO69_001382"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IVO69_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7400167.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IVO69_001383"
     CDS             12525..13103
                     /locus_tag="IVO69_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7400168.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IVO69_001384"
     CDS             13107..13628
                     /locus_tag="IVO69_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7400169.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IVO69_001385"
     CDS             complement(13707..14261)
                     /locus_tag="IVO69_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7400170.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IVO69_001386"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IVO69_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7400171.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IVO69_001387"
     CDS             14801..15052
                     /locus_tag="IVO69_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7400172.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IVO69_001388"
     CDS             15177..15968
                     /locus_tag="IVO69_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400173.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IVO69_001389"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IVO69_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7400174.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IVO69_001390"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IVO69_001390"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7400175.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IVO69_001391"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IVO69_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7400176.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="IVO69_001392"
     CDS             18151..18444
                     /locus_tag="IVO69_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7400177.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            19287..21614
                     /locus_tag="IVO69_001393"
     CDS             19287..21614
                     /locus_tag="IVO69_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7400178.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21740..22069
                     /locus_tag="IVO69_001394"
     CDS             21740..22069
                     /locus_tag="IVO69_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7400179.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            22109..23113
                     /locus_tag="IVO69_001395"
     CDS             22109..23113
                     /locus_tag="IVO69_001395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7400180.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            23110..24539
                     /locus_tag="IVO69_001396"
                     /pseudo
     CDS             23110..24539
                     /locus_tag="IVO69_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         8328 a         4411 c         4902 g         6978 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattta aaaataaaat
    19141 ctttgtatta aaggacaaga ctaaaataac cttagaataa aaagagaaaa aaagccccaa
    19201 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
    19261 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
    19321 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
    19381 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
    19441 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
    19501 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
    19561 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
    19621 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
    19681 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
    19741 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
    19801 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
    19861 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
    19921 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
    19981 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
    20041 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
    20101 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
    20161 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
    20221 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
    20281 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
    20341 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
    20401 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
    20461 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
    20521 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
    20581 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
    20641 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
    20701 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
    20761 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
    20821 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
    20881 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
    20941 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
    21001 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
    21061 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
    21121 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
    21181 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
    21241 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
    21301 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
    21361 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
    21421 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
    21481 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
    21541 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
    21601 gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
    21661 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
    21721 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
    21781 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
    21841 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
    21901 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
    21961 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
    22021 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
    22081 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
    22141 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
    22201 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
    22261 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
    22321 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
    22381 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
    22441 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
    22501 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
    22561 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
    22621 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
    22681 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
    22741 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
    22801 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
    22861 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
    22921 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
    22981 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
    23041 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
    23101 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
    23161 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
    23221 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
    23281 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
    23341 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
    23401 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
    23461 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
    23521 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
    23581 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
    23641 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
    23701 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
    23761 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
    23821 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
    23881 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
    23941 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
    24001 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
    24061 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
    24121 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
    24181 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
    24241 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
    24301 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
    24361 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
    24421 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
    24481 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
    24541 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
    24601 tgtgacaagt ggtcggtct
//
LOCUS       DACCPY010000041        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCPY010000041 DACCPY010000000
VERSION     DACCPY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.042"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IVO69_001397"
     CDS             complement(53..787)
                     /locus_tag="IVO69_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7400181.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVO69_001398"
     CDS             1114..1524
                     /locus_tag="IVO69_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7400182.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVO69_001399"
     CDS             1517..2218
                     /locus_tag="IVO69_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7400183.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVO69_001400"
     CDS             2218..3804
                     /locus_tag="IVO69_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7400184.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVO69_001401"
     CDS             3823..4044
                     /locus_tag="IVO69_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7400185.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVO69_001402"
     CDS             4041..4802
                     /locus_tag="IVO69_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7400186.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVO69_001403"
     CDS             5182..5691
                     /locus_tag="IVO69_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7400187.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVO69_001404"
     CDS             complement(5766..6305)
                     /locus_tag="IVO69_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7400188.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVO69_001405"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVO69_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7400189.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVO69_001406"
     CDS             7372..8169
                     /locus_tag="IVO69_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7400190.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVO69_001407"
     CDS             8197..8832
                     /locus_tag="IVO69_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7400191.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVO69_001408"
     CDS             8852..9625
                     /locus_tag="IVO69_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7400192.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVO69_001409"
     CDS             10033..10830
                     /locus_tag="IVO69_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7400193.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVO69_001410"
     CDS             10808..11221
                     /locus_tag="IVO69_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400194.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVO69_001411"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVO69_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7400195.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVO69_001412"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVO69_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7400196.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVO69_001413"
     CDS             15998..16558
                     /locus_tag="IVO69_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7400197.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVO69_001414"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVO69_001414"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7400198.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVO69_001415"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVO69_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7400199.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVO69_001416"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVO69_001416"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7400200.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVO69_001417"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVO69_001417"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7400201.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVO69_001418"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVO69_001418"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7400202.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVO69_001419"
     CDS             21905..22483
                     /locus_tag="IVO69_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7400203.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVO69_001420"
     CDS             22535..23017
                     /locus_tag="IVO69_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7400204.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVO69_001421"
     CDS             23134..23508
                     /locus_tag="IVO69_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400205.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACCPY010000042        23683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCPY010000042 DACCPY010000000
VERSION     DACCPY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.043"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(20..325)
                     /locus_tag="IVO69_001422"
     CDS             complement(20..325)
                     /locus_tag="IVO69_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7400206.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IVO69_001423"
     CDS             complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IVO69_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7400207.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(778..1044)
                     /locus_tag="IVO69_001424"
     CDS             complement(778..1044)
                     /locus_tag="IVO69_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7400208.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARSLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1299..2024)
                     /locus_tag="IVO69_001425"
     CDS             complement(1299..2024)
                     /locus_tag="IVO69_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7400209.1"
                     /translation="MSKKTTKAIVGIVAAVIAGTFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2385..2455)
                     /locus_tag="IVO69_001426"
     tRNA            complement(2385..2455)
                     /locus_tag="IVO69_001426"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2421..2423),aa:Gly,seq:ccc)
     gene            2572..3324
                     /locus_tag="IVO69_001427"
     CDS             2572..3324
                     /locus_tag="IVO69_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7400210.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3340..3819
                     /locus_tag="IVO69_001428"
     CDS             3340..3819
                     /locus_tag="IVO69_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7400211.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4070..5650)
                     /locus_tag="IVO69_001429"
     CDS             complement(4070..5650)
                     /locus_tag="IVO69_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7400212.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFIFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5643..6386)
                     /locus_tag="IVO69_001430"
     CDS             complement(5643..6386)
                     /locus_tag="IVO69_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400213.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6562..7338)
                     /locus_tag="IVO69_001431"
     CDS             complement(6562..7338)
                     /locus_tag="IVO69_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400214.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7586..8422)
                     /locus_tag="IVO69_001432"
     CDS             complement(7586..8422)
                     /locus_tag="IVO69_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7400215.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8433..9362)
                     /locus_tag="IVO69_001433"
     CDS             complement(8433..9362)
                     /locus_tag="IVO69_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7400216.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9554..9907
                     /locus_tag="IVO69_001434"
     CDS             9554..9907
                     /locus_tag="IVO69_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7400217.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9981..10637)
                     /locus_tag="IVO69_001435"
     CDS             complement(9981..10637)
                     /locus_tag="IVO69_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7400218.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10863..12797
                     /locus_tag="IVO69_001436"
     CDS             10863..12797
                     /locus_tag="IVO69_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7400219.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12883..14106)
                     /locus_tag="IVO69_001437"
     CDS             complement(12883..14106)
                     /locus_tag="IVO69_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7400220.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14100..15998)
                     /locus_tag="IVO69_001438"
     CDS             complement(14100..15998)
                     /locus_tag="IVO69_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7400221.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15992..17110)
                     /locus_tag="IVO69_001439"
     CDS             complement(15992..17110)
                     /locus_tag="IVO69_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7400222.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17193..18026)
                     /locus_tag="IVO69_001440"
     CDS             complement(17193..18026)
                     /locus_tag="IVO69_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7400223.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18019..18936)
                     /locus_tag="IVO69_001441"
     CDS             complement(18019..18936)
                     /locus_tag="IVO69_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400224.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19163..20287)
                     /locus_tag="IVO69_001442"
     CDS             complement(19163..20287)
                     /locus_tag="IVO69_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7400225.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20470..21378
                     /locus_tag="IVO69_001443"
     CDS             20470..21378
                     /locus_tag="IVO69_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400226.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21519..23132
                     /locus_tag="IVO69_001444"
     CDS             21519..23132
                     /locus_tag="IVO69_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7400227.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7379 a         4628 c         3954 g         7722 t
ORIGIN      
        1 tttctcctag ctttatagtc tattcttctt cttccgccat gaatgtattg aatacttctt
       61 caatcatgtc ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt
      121 cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagcag
      181 gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt
      241 catataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt
      301 cgtgggaatg gttatgttct gtcataagat tacctctttt cttttattat taaaataatt
      361 gcttacctga gccgtctaaa taattttgta agatcatcac agctgctaat ttatcgatga
      421 ctttttttcg tttggctctt gaagtatttg cctgctctac tagcatacgt tctgcctgaa
      481 cagtggttaa tcgttcatct tgataatcaa ctggcaagga gaatagttct ttgatctttt
      541 ctccgtatga catggaagct tctgcccttg gtccgatgga attgttcata tttttaggta
      601 atccaactac aaaccggtct acttcatatt gtgctactaa ttccttcaac cgatcaaagc
      661 caaactcttc ttctgcttca ttgatccgaa tgatctctac tccttgagct gtccagccaa
      721 aggggtcact gatagcaaca cctactgtct tagaaccgac atccaagccc attactctca
      781 taaattcact ccatgatttg agaggtagtc cttgacaatt acttccatga tctcatcgcg
      841 ttcgtgacgg cgaatcaaac ttcgagcatc acgataacga ggaatgtagg ccgggtctcc
      901 agaaagcaag tagccgacaa tctgattgat tggattgtag cctttttctt ccaaagcccg
      961 ataaacaatc gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa aaccaaaacg
     1021 tacggtttca tctgtaaaac ccatgattct acacctcttt cccgcaaggt aattccagct
     1081 taccttctac aaccttattt tactcgaaga aagggaaaac tacaaacaaa tattccaaac
     1141 ttccgaataa attaagaaac actatttgta tgtttttttc cttttttctg tagctttatc
     1201 aaaagtactc ttttcataaa gtatacgcta tatttctttg aatgtatgtt tttacacaat
     1261 tttttcataa tattcataaa aaattcgaat agctgctact aattttttgt ttttgttgct
     1321 tgtcctgtct cataattgat cgtgtagcca tcttgaacat tgtagataaa gacattgaat
     1381 tcaatttttt tgtcttcaat gctttctgct tccaattgca ctcccctggc taccagttca
     1441 ttcccctcaa aatgaggagt gacacggtag cgtacatgat gatttgtttc tttgatatat
     1501 gccgcaatat cattttcgtg agcaagcatg tatggagcat tcaacgaacg tgtccctgtc
     1561 atcaagtttt taatgttgtt attttgacca gtcagctgga aaccaattaa atggctgcga
     1621 ttatataacc attgcccgtc acttaatttc ttttgtttcc aacctgtcgg gtcaatatac
     1681 aaaggctctc tcttttcagt agggaacaaa tcttttccaa gcatagcatt ggcttgtcct
     1741 actctgttca atttatcaat atttgaatag ctttcccatg ttcctttgga aaggctaaga
     1801 tcctcttttg taaattccgg ctgattccca ttgatttcta tttcttgatg ttttccatca
     1861 tattctggca catccgctac ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc
     1921 tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac cgaatgtccc tgcaatcaca
     1981 gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac
     2041 taacagctcc ctaaatctta acatatgttc ccctataaat aaatactaga aagaaataga
     2101 aacagcaaaa aataaaacct gttttaagat ggcttaaaaa tacaaaaaag agacggattc
     2161 ctcgtctcta atcagtcacc actctatctg taaggtattt attattattc aaaaattttc
     2221 ttcatactca agacaataca ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt
     2281 gcatctgcgg taataagtcc atccaagtta aaaacataag aagttgtttt tgcttggctg
     2341 ttcttattac ttgttgcaga gcagcttttt cacaacctat tttaagcggg tgacgggaat
     2401 cgaacccgcg acatcagctt gggaagctgg gattttacca ctaaactaca cccgcaatac
     2461 aaaaacagta tacgcctctt ttattccttc ctcaagtttt tttattcttt acgcttctgg
     2521 tatgttttgt agtaaaataa aactaatcaa tcaattattg gagaagatgc tatgacggga
     2581 aaagaattag atctgttgct ggacacttgt ttactcgctg ggaaaatcat gatggaaagc
     2641 aatgcggaga tgtatcgggt agaagatacg atgagtcgaa ttgctttagc ttctggaaat
     2701 ttccgtttgg tcagctatgt cactcaaacc ggtctcttta ttggtcttga tcgtacctcg
     2761 accatccgaa tggaacaaat taccaaccgt tcgattaatt tagaaaaagt agtaaaagta
     2821 aatgatcttt cacgtaaata tgttgcttca gagctaacat tagaagaact ttactgcgaa
     2881 ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg
     2941 gcagtgatca gtggaacaat tatggtgtta tttggcggaa agatgaccga tttacctgtg
     3001 actttagtca ttggaggatt gggatacgca gcttatctat atagtttgaa attttttaga
     3061 atcaaatttt tatcggaatt tttatcttct ttattgattg gcatagccgc tatctacagc
     3121 gtacgttttg gcttaggaag caatcaggat ctaatcatta tcggatgtgt gatgccttta
     3181 gttcctggag ttcaaattac gaacgctatc cgtgatttgc tagctggaca ttatgtctca
     3241 ggagtttctc gcggaacaga agcgatgatg actgctacga tgatcggttt tgcgattgct
     3301 tttattttcc agctttttta ttaggaaagg aacacaagta tggggacgat atttattcag
     3361 ttttctttta gttttttagc gactgcggca tttgccgtta ttacaaatgt acctaggcgt
     3421 tcattgattt gttgtggtct ttctggtgct tttggctgga tgatctactg gatctgtgtt
     3481 caaataggcg gatctgctgc ttttggttca ttgcttggtt ctttaggagt agcattcatc
     3541 agcgatcttt tctctaaacg tttgaaaatg cccgtaacga ttttcaatat tcccgggatg
     3601 gttcctctag tccctggtgg tttagcttat caagcggttc gaaatctcgt tactgaatcc
     3661 tatcgagaag cggctcatta tgcggtccaa gcaattatga ttgctggagc aatcgcttta
     3721 ggtcttgtat tatcagaagt ctttaaccat aacatccgta atttcaaagt aaagcgtgac
     3781 gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag
     3841 gttgtgaaag agacgttcag ccttgagcga tgacaaaata tctcacaaac attgcgacaa
     3901 gtaaccgcag gagcaataag aagtaattat gagtattaaa aaaagttttg taaaaatagc
     3961 ttctcacatt tttacataaa attgacttaa tactgagaaa ctgacttttg aacaccctct
     4021 atctgtaaaa aaggggctgc gacatgattt ttgtcacggc ccctcttctt tattcaaatt
     4081 ttttccaaaa cgttcgttga taataaacga tccatagagc ggaaagtatt agaactaaac
     4141 cgatcacagg aatattcact aaccaaaatg gataaaacaa agccaacatt ccgtataaga
     4201 cgataagaat aatactgatg atcaatgaac ccatcatagt tgcgactaaa cccaaacttc
     4261 ctgaacctct gttaaataat tggttaatat tggtccagtt cagcagcagc aaccgataat
     4321 ctctagcgaa ataacgtagt gacaataagt agcagccgaa taaagaacca aatagcaagc
     4381 tagggataaa aaacaaaggc agctgaaaag acagtccgct taataatgcg atcccaccag
     4441 ttaaaaaact ctgcaatatc catccgaatc tgaacttttc ttttaaatat tggttcatag
     4501 agattggcaa agaacggata aacagaaaat tttcttgatc caaagaaata agattgctga
     4561 taaaagaagt ctgattaact gttaagaaag ccaatgcgat ccctaccagg aaaacaacac
     4621 cgattaactt attatctaaa tcagttaatt ttatttcgcc tcctagggca aaagcaagaa
     4681 taaaaatgat tggagttaac aaggaagaag aaagaacttg cataatcaaa ttaggttctt
     4741 ttactaattg agcattgtaa ctaaataaaa gctgatgcac attttggttc tttttatggg
     4801 ttctttttat tttgaactca ctgtttgaat cagtcagttg ctctgtaagc tttggtacta
     4861 tcagctgttt tacagcaagg aaaaatccca gagccgtgat agctagtatt ccccaaccca
     4921 ataaaccttg ttgagaaagt ggctgactgg cagcatcata gaatggcaat aatatcgaaa
     4981 tcggatgccg atctgttaac tcattttcaa tagtccccgt ttgatgattc atccacatga
     5041 ttccagcaaa tgcgatcagc atcgagctgc caagcaagat actagaaact gtttttctat
     5101 gttttttaaa cactgctgtt cgagccaaac caaaaacaat caaactagcc agacaaaaaa
     5161 gaataatcaa aaatagagaa aacaaaagga ttgagataat gatagtaagc aaaatcccta
     5221 gatcagctcg ccatccggtt aaaataaaca tgactagaag cggaaaaata aaaggaatga
     5281 tggtcaaagc aactaccaaa atctttgctg aaaaaacctg ttcttgtcca aaaggcagag
     5341 gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt ataaatgact gatatacttt
     5401 gagttaaacc taacatacca aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa
     5461 caaagttcat agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa
     5521 gattttgaga caaaagatag atagttaatc gctttccctt ttttcctttc tctcttgctt
     5581 tattcgtgag ctgtggatta acatataaaa gattaactcg aattaattct aataattgtc
     5641 gcttatacat acggtccatc tcctgtcgct tgccttccag ccattttcaa ataaatcttt
     5701 tctagcgttt catcaggtga gccattcaat aattcactaa ctgccccctg ataaatcaat
     5761 tcacctttct tcaaaatagc cagtttatcg cataactgtt gtgcggtatc caatacatgt
     5821 gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct tcatcatttc tttcaaatca
     5881 aatgctgctt gaggatcaag tccttgcaaa ggttcatcca atacccaaat atctggatct
     5941 ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa acttgcgatt
     6001 gtttcatttt gatgactata catatcaaaa agcgctgtca attctgctaa acgcttttct
     6061 tttttttgtg tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc
     6121 aattgtaaaa aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat
     6181 cgattttccg tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata
     6241 ctaactaaac ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa
     6301 atttcccctt gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt
     6361 ttacttacat tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat
     6421 tgtacccttc gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga
     6481 aacgactagg ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta
     6541 agatatgagt ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt
     6601 ctttagttta tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac
     6661 tatttcgttt actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa
     6721 atctttacat aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac
     6781 gagaatctct tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat
     6841 tagataaagg atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt
     6901 ccaccttttt ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact
     6961 ttggatgctt tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt
     7021 aatctttcga ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt
     7081 acacttttca ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt
     7141 caaatcgatg tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc
     7201 aacaaattgt ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat
     7261 tctggattct tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat
     7321 aaatatacgc gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc
     7381 atacaacttt tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca
     7441 acacctttga aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac
     7501 aaatgaatac atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg
     7561 agctatgttt atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag
     7621 cagagacttt ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat
     7681 aatagtctga ttggatggtt ccttctattt tataccattc atcattttta accgttactc
     7741 cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc
     7801 cgaatcggaa caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt
     7861 catagctgat ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt
     7921 ctaaatagtt ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact
     7981 cattcatttg gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt
     8041 attgtgtctg catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt
     8101 tggcttctac gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg
     8161 gcaaagctag cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat
     8221 gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa
     8281 ctaaagccaa aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat
     8341 tgatatattg atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag
     8401 ataaaatcaa aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg
     8461 ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc
     8521 atcagtagat ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct
     8581 gtgccgaaca gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac
     8641 atcgtaaagg ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt
     8701 agcataattc tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc
     8761 aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga
     8821 tggatcagct gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga
     8881 tggtgatttt ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa
     8941 taggcaagcc agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt
     9001 gccatctgcc aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg
     9061 atcggagcag tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat
     9121 tgatgaacga tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa
     9181 ccagtaaaaa tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc
     9241 aaaaatactt gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca
     9301 ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac
     9361 atgagtttcc tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa
     9421 aaaaaacaag ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa
     9481 ttgacttttt ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa
     9541 agggagaata aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca
     9601 gtcatccgtt gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg
     9661 tccacgacta gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt
     9721 tgaaacacca gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt
     9781 tcatcaagga aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta
     9841 tctactagat gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac
     9901 aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt
     9961 tgttcaatcg ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa
    10021 tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca
    10081 tctgacggag cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc
    10141 gttaactgat tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg
    10201 atttgttgga tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct
    10261 acgatgcgcc caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta
    10321 ctttgatgga atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca
    10381 acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca
    10441 tcataaccat acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt
    10501 gcactatcta cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca
    10561 ttatttaaaa tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga
    10621 ataatatgat ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc
    10681 catcatagca gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta
    10741 ttctttattc atttatttat catttctgca aatctcgcag tataatggaa tttatggtag
    10801 actactaatt gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt
    10861 ccatgatatt attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg
    10921 aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg
    10981 ctggaaaatc gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga
    11041 tcgctaaaaa taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca
    11101 atgaaacagt ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc
    11161 ggatgcgcga gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt
    11221 cttatttaaa agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt
    11281 atgaaaatga aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc
    11341 agaaaatcga tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt
    11401 tgcaacaccc tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac
    11461 tttcctggct tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg
    11521 accgttattt tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc
    11581 gccattacaa aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg
    11641 aatggaaggc ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc
    11701 ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa
    11761 aaatggaccg gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta
    11821 tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata
    11881 atgtcacctt atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag
    11941 taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt
    12001 ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc
    12061 aagctgatct gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca
    12121 ctccagaaaa agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag
    12181 aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag
    12241 cgatgaacaa agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca
    12301 acaaagaagt attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc
    12361 atgaccgata tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca
    12421 gcaaagtcta tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa
    12481 tcgccgaatt gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg
    12541 attattacca aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc
    12601 aggttgaaga agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg
    12661 tcgcgccaga aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag
    12721 ctgctagaca agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag
    12781 aagaatatga aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt
    12841 ctttttttat tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca
    12901 attactagtc attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt
    12961 atttacccca ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc
    13021 ttccgttgta gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta
    13081 taagttcttt tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac
    13141 catgatcacc atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc
    13201 ccaataacaa atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct
    13261 taaatcaaaa tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc
    13321 ccatgcttgg cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata
    13381 tcttggtttt ccatctttga catccatata aaatgtatga taagttgaac catcttctct
    13441 tactaaataa tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt
    13501 tccagtttct tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat
    13561 tctaccttgt tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc
    13621 ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt
    13681 tcccgttatt ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg
    13741 attagtgata atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt
    13801 ttgttgaaat aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa
    13861 tccttctgtc caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct
    13921 tgggaattga tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca
    13981 acgctcgatc tcattattcc attctttttc cattaattct ttataacatt catttatcca
    14041 tttttctgga tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt
    14101 tagcataatt ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca
    14161 tcaaaatcaa ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga
    14221 gcaaccactc ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga
    14281 ataaaagtgt tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat
    14341 tttcgtttaa tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc
    14401 ctatagtttt ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata
    14461 gcaaaccctg tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc
    14521 tgccagcctt caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat
    14581 gtagaggcaa gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac
    14641 tgtccatagg ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc
    14701 tgaatcccta gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc
    14761 gaaaaaccaa attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg
    14821 aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg
    14881 agcaaattgg cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta
    14941 gccgtccaat actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat
    15001 aatggagata acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca
    15061 atattcagga tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga
    15121 ttgactaacc ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct
    15181 gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca
    15241 ccatcttccg caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat
    15301 atctggctaa tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat
    15361 gcaaaaggat tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga
    15421 agataacaat tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa
    15481 ccaaattttt ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca
    15541 cattcatatt caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct
    15601 aattttttcc ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct
    15661 accatacgct ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt
    15721 ccagctttta ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg
    15781 ggccccgctc cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat
    15841 cctacccctt gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt
    15901 ggttgattta tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact
    15961 ggatgtttca ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta
    16021 atcgaagtaa gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca
    16081 gttctctatg gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat
    16141 ttgcatagtg cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg
    16201 atcgtacaga ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag
    16261 cactggtatc aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt
    16321 tttcgatttt ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat
    16381 tttttgtgta acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc
    16441 aacaagaatt ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg
    16501 catcaaattc tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg
    16561 tatctgcatg ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc
    16621 aatacagtaa tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat
    16681 tccatgctac taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat
    16741 gtaaccctag ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg
    16801 gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta
    16861 ataattgttc ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga
    16921 gctgccataa aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta
    16981 ttacgtctgt atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta
    17041 attccgcatc tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct
    17101 gttcattcat ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga
    17161 acgaactaga tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc
    17221 tccttaaatt tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct
    17281 aaaattagca taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt
    17341 ttgataatta agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga
    17401 tattgattcc aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa
    17461 atcggtacag cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc
    17521 tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta
    17581 ctggcaatcg aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata
    17641 atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta
    17701 ttgaacaaca gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta
    17761 gaactggctg tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc
    17821 aagggataat tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac
    17881 ttgaaattga ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata
    17941 tataaatatg gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt
    18001 ctgctttgta tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga
    18061 taatataaca gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac
    18121 catttcaaaa cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc
    18181 attggaagtc ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc
    18241 taaaactgtg gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg
    18301 ccgaaatttc gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg
    18361 aagaccagct aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat
    18421 cattgatatt cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc
    18481 attcaaaaat ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac
    18541 agccaccgtt gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt
    18601 taactgtgct gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat
    18661 tgatcctgcc atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa
    18721 gatagctaga taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact
    18781 aaaagcaaac gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg
    18841 tgtgaccaat ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt
    18901 ctttattgtc ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt
    18961 aatcaccagt taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg
    19021 aattttcaaa atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag
    19081 catctgaata agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata
    19141 gttcatagta tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat
    19201 aggccaatga gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat
    19261 ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga
    19321 cataaaaact tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa
    19381 tcagtggagc catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag
    19441 cataccagcc gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg
    19501 caccgtagaa atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt
    19561 tcgcttcttt agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct
    19621 gggctagcat gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac
    19681 taccagtttt ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt
    19741 cttcaaaatc tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg
    19801 taaaatcact agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca
    19861 atttttgatc ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat
    19921 aaaaagcatc ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa
    19981 tatccggcat atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc
    20041 ccgaagctac attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt
    20101 ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag
    20161 tggctttttt tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac
    20221 atgcacttaa gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt
    20281 ttttcatgtc tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta
    20341 tattaacgga attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg
    20401 cttttcattt ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga
    20461 gaaaggaaag tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca
    20521 ctaacctatt ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta
    20581 ataaatgaaa gcgctaattt atatgctgaa tttgcacata aatctataga actagataat
    20641 ccttcccttt tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa
    20701 acacaaactt ttatcattat ccagttttat tttgatgagc aattattagc acatattacg
    20761 ggacaatctt tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa
    20821 cagttcaaaa ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc
    20881 aattcttttt tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact
    20941 tattgtctgc aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct
    21001 ttattgacaa taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca
    21061 gatgaattag ttgatccaat catacgatat ttagaagaac atctttcaga aaattttttg
    21121 cttgaacaat tagcccaaat tttttttatc agtccagcca gcttaaaaaa acattttaga
    21181 aaagtgaatg gaaaaagtgt tattagctat tttaaagaac taaaaatgga aaaagcaaaa
    21241 cgtctcgtgc tagaaaatga attaacctat acagaaattg cagatatatt aggatttcat
    21301 tcgatccatc atttttcttc agcttttaaa aaatatacag ggttatcccc ttcaaagtat
    21361 tatctatcct taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat
    21421 tttctgtcta ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca
    21481 taacatagta cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg
    21541 gtgtagctgg cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa
    21601 tcattatcta tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt
    21661 taggaggtgt gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa
    21721 caaaaaacaa catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta
    21781 agcgattaac agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt
    21841 tgatcatctc ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag
    21901 aaaacggctt tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag
    21961 aaaaaaatcc acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg
    22021 agaatttcaa gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt
    22081 tcccttatga tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag
    22141 ccgttcattt agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat
    22201 cagatggctc cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga
    22261 atgaagtggt gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg
    22321 accagttaca aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta
    22381 gtgtagtgac tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac
    22441 atatgctggc agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag
    22501 gtgtcgcaaa aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa
    22561 aagcatcagg cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt
    22621 tctatccaga agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta
    22681 ttttaggtgg acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg
    22741 tagcaatcac cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc
    22801 caccgtattc tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa
    22861 tgacgaatat cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag
    22921 cgttcttttt agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa
    22981 aaaatattcc aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag
    23041 tttacattac tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt
    23101 tgggtatcaa agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt
    23161 ctaggagtta tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt
    23221 atgtatatag aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta
    23281 ggttatagtt taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc
    23341 ataaaaaaac atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta
    23401 actacgccta atattacgag gcatcatatt aatctatcag cactagtacc taaactagca
    23461 tccctttcga atattttttc ttatataata aagtttgctt tcactcctct aatctcacca
    23521 aatcgactgg cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat
    23581 tgtaatcata tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat
    23641 gtgtgtatta aataatttaa tttttttgtt cctatttatt taa
//
LOCUS       DACCPY010000043        23466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCPY010000043 DACCPY010000000
VERSION     DACCPY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.044"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(273..2458)
                     /locus_tag="IVO69_001445"
                     /pseudo
     CDS             complement(273..2458)
                     /locus_tag="IVO69_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            2676..3359
                     /locus_tag="IVO69_001446"
     CDS             2676..3359
                     /locus_tag="IVO69_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7400228.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3440..4159)
                     /locus_tag="IVO69_001447"
     CDS             complement(3440..4159)
                     /locus_tag="IVO69_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7400229.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4471..4677)
                     /locus_tag="IVO69_001448"
     CDS             complement(4471..4677)
                     /locus_tag="IVO69_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400230.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4921..5523
                     /locus_tag="IVO69_001449"
     CDS             4921..5523
                     /locus_tag="IVO69_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7400231.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5664..6944
                     /locus_tag="IVO69_001450"
     CDS             5664..6944
                     /locus_tag="IVO69_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7400232.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6957..7685
                     /locus_tag="IVO69_001451"
     CDS             6957..7685
                     /locus_tag="IVO69_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400233.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7754..9277)
                     /locus_tag="IVO69_001452"
     CDS             complement(7754..9277)
                     /locus_tag="IVO69_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7400234.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9529..10710
                     /locus_tag="IVO69_001453"
     CDS             9529..10710
                     /locus_tag="IVO69_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7400235.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10770..12398)
                     /gene="treC"
                     /locus_tag="IVO69_001454"
     CDS             complement(10770..12398)
                     /gene="treC"
                     /locus_tag="IVO69_001454"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7400236.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12795..15506)
                     /locus_tag="IVO69_001455"
     CDS             complement(12795..15506)
                     /locus_tag="IVO69_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7400237.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15516..16217)
                     /locus_tag="IVO69_001456"
     CDS             complement(15516..16217)
                     /locus_tag="IVO69_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400238.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16403..17005
                     /locus_tag="IVO69_001457"
     CDS             16403..17005
                     /locus_tag="IVO69_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7400239.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17086..18099
                     /locus_tag="IVO69_001458"
     CDS             17086..18099
                     /locus_tag="IVO69_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400240.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18208..19125
                     /gene="rbsK"
                     /locus_tag="IVO69_001459"
     CDS             18208..19125
                     /gene="rbsK"
                     /locus_tag="IVO69_001459"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7400241.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19153..19548
                     /gene="rbsD"
                     /locus_tag="IVO69_001460"
     CDS             19153..19548
                     /gene="rbsD"
                     /locus_tag="IVO69_001460"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7400242.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19548..20921
                     /locus_tag="IVO69_001461"
     CDS             19548..20921
                     /locus_tag="IVO69_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400243.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21278..23422
                     /locus_tag="IVO69_001462"
     CDS             21278..23422
                     /locus_tag="IVO69_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7400244.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6845 a         4594 c         4211 g         7816 t
ORIGIN      
        1 tataaaaaaa aatcaaaata actgattatt tacgtttgaa taatgagaat ttttctatat
       61 attctctatt ttgataatag tattctcatt ttcaaaataa ataagggagg tgtgacaaaa
      121 ttcttgtcac acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat
      181 aagctgaaat ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga
      241 aactgaccac ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg
      301 gtttctttga taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat
      361 agatacagac gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct
      421 atcgtcgtca ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct
      481 ccgttataag gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga
      541 cctttacttt tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca
      601 atcaagcggg tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt
      661 cttctgaaag tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg
      721 atttccttag ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga
      781 tgctggtctg ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt
      841 aaattggggc ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa
      901 aagtattgat cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat
      961 tgctggtagg cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt
     1021 tgatagaaat gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc
     1081 agtacaaaag gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct
     1141 gatcggtata atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt
     1201 tgtttgatta gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca
     1261 cctgcttctt tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg
     1321 atcaatctat tgctccttgg acaatagctg cccgagaacg ataattagtt aataagaaaa
     1381 gagttttggt aggatattct ttttcaaagt tcttgatgta tgctggctct gatcctctga
     1441 atccatagat actctgatca tcatctccaa caacaatcaa cttgatcctg ttttcctttg
     1501 tcatccgttt cacgatttcc cattgaagtg gattagtatc ttgaaattca tcaatgaata
     1561 gataatcata tttttggccg atataatgct ggatctcctc attctccaaa gcgcttttca
     1621 gattttggag tacatcattg aactctagca attgctggct tcttttttgt tcttggagtt
     1681 ccgtgagcaa tccatacagt cgttctgata tcggctgtcg tgcattctta tcaatggaat
     1741 catatttttc tttaactaac tgaaccattt cttctactga aaggttcata ttctgccaat
     1801 agtctaaccg agaagcaatc gttttttctc catattgatc aaatatataa agattatatt
     1861 tacgtatcag ctgttgcaaa acagcattca ctttttgtgc agtatccttt gaaaagtctc
     1921 cttgtattcc ttgcattgct acttcaggaa attttcgaca aatttcattg tatatcctaa
     1981 aaaagatact atggaatgtt ccgatatgga cagcggtttt tctctggctg aaattatcta
     2041 ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt gatcattagt atgcgttctt
     2101 cgtctacttg tttttgcgtc atcagataaa cagcttttgc acagattgtt tgcgttttgc
     2161 cgctcccagc tcctgctatg attcgaagat gtttcttcat atcatatgtg atagctgctt
     2221 tttgttcttg attgaagcga ttataggaac gtttctccat cattttaaag aaagagctgt
     2281 ttccttcact ttcttttact tgtacttctt cctcaataag tccgggtgct tcgccataag
     2341 ccgcttcata ggtagcaatg atttcttctt gtagccgaaa gacttgttga agctttttat
     2401 acttgttctc ccagcgtttc aattcttttt gtaatcggag aatttctttt tcattcaact
     2461 gattgatttc cttcccatct ttcttctttg aatttgttta tttcaagttg gttatttaaa
     2521 gtttcgttgt ctattttttc tttatactaa cagacctttc taaaaatgaa aatgggaagc
     2581 gatatgattt tagttttctg aaaatttttc ctttttttca atcgtttatg ctaagataag
     2641 gaagacaaaa atattcataa aaggtgatga aaaaaatgca gacttccttt cacgaggggc
     2701 gtacaatagg acttttgctg acatttatcg gaggagccat ggacagttat acttatatcc
     2761 aatatggtgc ttttgcctct gctcaaactg gcaatatcat tctatcgatc atccaagcat
     2821 ttgacggaga atggctaagt gttggtaaaa aagtattgtc tacccttttc tttttcttag
     2881 ggattcttct aacaaagtat ctaattgatt attttagaaa aaaagaaaaa catttctggc
     2941 gcttgtttgt tctatattat gaagcagtga tttttttctt tgttagtttg acacccgtcc
     3001 atgagcatcc cgcattagta acgattttga ttgcttttac tgcatcgatc cagtgggtag
     3061 cttttgataa aatcaacggg cgtgcctaca ctaatttatt tacaactgga aatttaaaag
     3121 gggtagcaac gaacttatat gattattgta tttctaaaga aaaagcagat tttgatcgat
     3181 ttttccacta tctaagtgtc gttcttgcct ttatcgcagg agctattgtc cttgttttct
     3241 gtcatcatct gtttggcgcc cattcgattt tgatcgtttc cattttattt ttgatcatgg
     3301 ccctcactca gacatttcat ttatggcaat tttatcatac gaatcggttc actatttgat
     3361 ctcaatgaat caaaaaaact aagcaagatg ctgctcacag agaaatatct gcgagtgagc
     3421 aatttgctta gtttttatat tatttagttg taaagattcc ctttgctttc gggttatttg
     3481 tagtgtagcg gatcgactgg gcatctgcct cttctgtcat tttctttgcc cgcatcttga
     3541 tttcttgtac gatttgttct ccacatgccg gatcggctac gaaatcatat ttgtccccat
     3601 cgatcactat tttgggacat atatcaaact gttcatacca ctcttcgtat cgtcgattga
     3661 gttctttata gtaggaatag agttcaggat cttgttctag ctgttcatat tcccggcctc
     3721 tttttttgat tctcgctaac atagtttcaa aggatacacg aatataaacc agtagatctg
     3781 gtttcttttg tggcgaaatt tcttctaact ccttcagcat cgtttctaaa agaccttcat
     3841 actgctttac ttctacttca gaaacacgcc ctaaatctgc attcaaatga aacagcatac
     3901 tatcttcgta gatggagcgg tctagtacgt tgtcatcatg gctgaaagca tctttgattg
     3961 ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa agtatacttt tctggattag
     4021 aataaaatag cggcaatact tcattatctt ccacgttctc ataaaaaggt tttgtttcta
     4081 aatcttgggc aagcatttca gttagtgtgc ttttccctgc accgatcgtt cctgcaacaa
     4141 tgatcacaat tatcggcatc cttcctgtaa taaaatctgt tcttcatttt actacttcat
     4201 ttatagaaat aaaagccttg actttatctg ctccttaaaa gctacttctc taaaagtagc
     4261 caaaagtaac tcctattcat gaaaataaag gctgtgacaa aagtcttgtc gcagcccttt
     4321 tttcaaataa acagtattct cctgccaaca cctcggtcac aaggcaatat tcataaaaca
     4381 tgaaaagctg ttttttaaca ttattccttg taactcagag ctaaacggga gactcacaac
     4441 ctctttatgc tctcaacatt ttatctgttt ttattcttct attgtacttg atttgcgtac
     4501 aactaactgc cagtgcagat tatgagcggt atcattgtct tctactgcgc atttcatgac
     4561 acgtatcatc acactatttt catagatttt ttctattttt ccttttattg gtgattcaaa
     4621 taaatcagat ttaaatgcat atgtttcgcc gacttcaaac aattgttcat tttccataag
     4681 aatccctcca tcacctttta taaggtcagt aattttttaa cataaatcac tcgaaaaagc
     4741 acaaacttgt cataaaaagt acttttcgta tcaaaactgt cacttttttt ggtaaggttc
     4801 ttggaaaacg aagatttttt taggagatca tgacggtttt gatgtcaaaa ccacttttta
     4861 tgaaacaaaa gacgtttttt atttttttag cataaaattt gtggtggagg tacgtatgag
     4921 atgaaagata aagttgtgaa caaattatta gaagccttat tcagcggaga caaatcggaa
     4981 gaagatatgg tgtatgtcca agtaaaattt gccttagaag ttttattgaa taatcttgga
     5041 aaactagcgg tcgttctagt cttttctctg gtgacaggct catgggcgga aacaggaatc
     5101 acctttcttt cttatatctg catccgcaga tatgcttatg gattgcattc cgactctgaa
     5161 ttcgtttgtc tgctgtggac actactttat ttatggggcg ttcccttggt gatgaaacat
     5221 ctgcaattga ctatctcctt cccaatgatg gtctttttgt taatcagctg ctttttgtta
     5281 ctgcttcgct atggatcaag aggtacagca atcaatccca ttgaacccga gaaacgtccc
     5341 ccattattaa agaaggcgat cagcatgttt ctaatttttt ctctcattac tttatttttc
     5401 tcagcttctt acttttctac ctatttgcta cttgggattg ttttagaaat agctacgttg
     5461 cttcctatta caaactactt gatgaatttt ggaggaatct ttcatgaaaa aaacaatcat
     5521 taaacaatta actaacttat ctgacttttt tgtatctggc tgctgctttg gtatgagttc
     5581 tgaattcaaa atccctgaaa aattaaaaaa ataatacgct aattgtgcta taatttggta
     5641 aaaatcaatg ggagctgtac tttatgtttt tatggtccat agcgttgcaa tatttgattt
     5701 acacaatttg tatcttattg attgaccatc acatccgata caaatatctg tttgcgggag
     5761 tcattctgat ttcttgtatt ttgattggat gggtctattc gattttaggg atttttactg
     5821 ctctaattgc tataggaatg ctgatgctta cttttttctt cttttcgact tcgcgtcaac
     5881 aattggttta cgcgcttcct tttgggttat tgacgtcgct tttaggagat cacttaacaa
     5941 gcatcggcga tttttttgtt ttgaaatctt cggttattga accaggagat tcattgcagt
     6001 attttcacat tgtcttatcc gcttttttat ctgctttatt agctttttgt ttaaaaaaat
     6061 cgttagagac ttggtttcac actgtggacc gccgtatgat cggtacgatc gggacattga
     6121 tattatttac atactatatc attatttttt atacccgttt ttctggtgaa acacctgatg
     6181 tcttagcttt gaatacgagt tttttcattc tatatctgtg tgttggcctg atcatcttgg
     6241 tttattattg gaaaatctct aataagaaat tagctgatca gttattagag caacaagtca
     6301 ggatgcaaca ggattatatc aaagatctag aaaaaaacta tcaagacttg agagaattca
     6361 aacatgatta ccaaaatctg cttttttctc ttcacagcta tatttcagaa ggcgacttag
     6421 aaggattacg gtcttattat gatcaaacga tattacctac acgccagatg atgaaccttt
     6481 ttccggccaa tctaagtatg ttggataata tgaaagttcc tgagatccgt agtttactgt
     6541 cattaaaact catgatggca caggaaaaag ggttgatcgt ccagcttcat tttcctgagc
     6601 caatcgaact ggatacgaaa catacggtaa accttgtacg tatgcttggt attatcttgg
     6661 acaatgcgat tgaaggagct agtgctgcaa agaagaaaaa aatcgaattg accatactca
     6721 agaagaatca ttccgtgatg attcgtgtga tcaatacaac aacaaataca ttaccgttaa
     6781 accagttgaa acaaaaaggg ttctcaacaa aaacaaatcc aaaaaacgaa ggccttggct
     6841 tgtttatctt ggatgaattg acaaagacca atccttatct tttccttgaa acatcaattg
     6901 aaaaccagcg tttttctcaa acgatttata tacaaataaa ataaaggagt aatgatatga
     6961 ttcccattta tatttgtgaa gacgatccaa aacaattaga agttttatca acggtgatca
     7021 aaaatagaat catgattgaa aacttagaca gttatgtcca tatttctacc tctgatccgg
     7081 aagaacttct caatgcagcc aagatcagaa cttcttcttt tggtatttac tttttagata
     7141 tcgaactaga agacagtgaa atggatggtg tagcgatcgg taaattgatc cgtgaattag
     7201 atcctttagg aaaaataatc tatgtgactt cccatacgga aatgtcgatg aaaatcttaa
     7261 aaagcaacat cgaaccgact gattatatcg tcaaagaaga tctttatgat ttaaaagaaa
     7321 atgtcgagca tatcctgagc aaaattttcg aagattttca aatgtcccaa atccaaaaag
     7381 atatcttcaa aatagagttt aatgatgaga tcaaattttt accgatcaaa gacatccaat
     7441 atttttctac ttcgctagga gcgccacata aactggaagt ccatttgact catgcccaat
     7501 tacaattcta tgagaagatc aaagaaatcg agaaaatgca tcggaatttt atccgttgtc
     7561 ataaatccta tgttatcaat acacaaaatg tgcggtcgat taataaaaaa accagagaag
     7621 tcacgttggc aaacggcgag accatcccag cctctatccg tggattaaag aaattgattt
     7681 cataaaaaga accgaatagt ttggaccgac taaatgacca gccgatccaa acccttcggt
     7741 tcttttttgc ctatcagtgc aaaagcattt cctgtgcaat ttcacgtcca tccattgtat
     7801 ctggataaaa tgttggccaa ttctccattt ctttcagcaa tacttcttga ctttcatttc
     7861 ctgcatataa atgaaaatgt tcggagtcag ccggtgcaat cgtgtgatca ctgaattgaa
     7921 tatatctaaa agcgccactt tccttatctt ctgcttcgaa tagatacctt acgccacggt
     7981 ttcctgcctc gtaggtcaag atttctttcc ctacataacg ataatttgaa cgtttcgttg
     8041 ttccatcctt aaacgtaaat gccatctctt tctcactgat cttgattgaa gcaatatctg
     8101 tttgatagcc cttcgtataa tattctttat attcttgtgc tgtcttatcc tgattcaatt
     8161 tttctttata ttcaaaaaca ggatcaaaag tcccatcttc tacatacgta tctactgttt
     8221 gccaagttcc tgcccaatct gaaagagagc gatcttgtac atcctcgtct tcaaaataac
     8281 cgttgtagac gctcttttct tttttggggg tcaaagtatc ttctagcggg ttttctgtct
     8341 ctgtcgtttt tttcaacgct tcgaggtttg cttccatgat agaaagatag ttttctccat
     8401 tttctatttg ttcattcgtc aatccttcca aaggattcaa tacttctaaa gatacacctg
     8461 cttcgtttgc taacgttctg gcaatactat ctttggcgtt ttcttcaaaa taaatatagt
     8521 tgattccatg ttctttaaca aagtgtttca gttcagcaat tctcgccgca gacggttctt
     8581 cactagaaga aagaccagca atcgatactt gattcaatcc atattctaac gctagatagt
     8641 tgaatgcagc atgttgcgtg acaaaagatc tttgttttgc ttgcttaaag gtttcttgat
     8701 aaagctgatg gagtgcttga agtttctcgg tataggctgc tgcatttttt gtccatactt
     8761 cctgtttttc tgggtaagct tcaatcagct gatcacgtat gtttgctacc tgcttgattg
     8821 ctaaacttgg agctagccat acatgtgggt ctagctcatg tgtatggcct tcttccccgt
     8881 gttcatggtc atgatcatgt ccttcttcac ttcccggaag taaaagcata ccttctgtcg
     8941 cttttatcgt atggacattg ccttcttgta atgttttttg gattgcgggg acccatgttt
     9001 ccatgttttc atcattataa acaaagatat ctgtatcttg gatctttgcc atgtctttag
     9061 cagaaggttc ataatcgtgg gaatcagtcc ctgctggaat caataactct acttctcctt
     9121 catccccgac gattgctttt gtaaaatcat acatgggata aaaggtcgtc atcacgctca
     9181 atttttcttt actgtcctcg gctttcccac ttgccccgca agcagttaac atacctgcta
     9241 aaaccagcat ggccaatata cctatcaatc gtttcattta tgacactcct cttctcatct
     9301 tctttcattt gaatgttccg attcgctttt cgcaaaccgt aatagttacg ttttagcaac
     9361 aggatttaat ttatcatatg gttaaaagaa ctgtcaagcg ttttcttatt ttaattgatg
     9421 agaaagttta tatgaatgat tgacgatatt tttgcaatca acctctatca ttttcctttt
     9481 atacccgctt ttgtctataa tgaagttata gataaaggag gaaaaagagt gaaaaaagaa
     9541 acagatacaa cgaatcgttt gatccgcttt ttaggaggaa aaacctctta ctatgtttta
     9601 ggaatagtta tcttacttgc gctgatcatt tattttttcc atcagatctc gtttatattc
     9661 aagcctgtcg gaatcattat cgctactatc ttgcccccat tattgtttgc actgatcttg
     9721 tattatcttt tggaaccagt catcaaacgt ttgagcaagc acctttcgag gaacttgtca
     9781 gtcattatcg tttatgtgat tattttattt ttattggggc ttggaagcat atggcttatt
     9841 ccttttcttg aagaacaggc aaagacattg atcagtagtt tgccagattt gtttcaagat
     9901 tttcaagaga gtctccgaca atttttggag aaaacacctt ttgccgattc atttaatcaa
     9961 tttttcgctt ctattgacga cgtgacgtct gatatagctg gatttattgg aaattattgg
    10021 gaaagtggcg cacaacggat cagcaacatt ttttcaactg ttacagctat ttttattact
    10081 ttatttactg gtccaattgt cgcttttttc cttttgagag atcctcaaaa attttatcag
    10141 tcagttttag cgattgtccc accagcattt cgtacagatt ttaaaaattt gacaaagatt
    10201 gctaatcaac aattgggttc atttctaaag gggcaaatca ttgcttcatt tatccttgga
    10261 gcagtctact gggtttgttt cttactgatc ggattagaat ttgctagtgt tttagctatt
    10321 tctgctgggt tgttatgtat cattccatat attgggccgt ttatcgcctt ctttccaggt
    10381 ttgatcattg cattccaaga ctctactttt atggctgtca aatttgtcat tgtctggttt
    10441 gccgtacaac ttctccacgg tgacttggtg attcctcgag taatgggtga tcgtttacag
    10501 atccatccga ttacgatctt aatcgtttta cttgtcatgg gtgatctaat gggaattgtg
    10561 ggcgttattt ttggtattcc tatctatacg ttagtcaaat tactcgttac ttttattttc
    10621 cgtaaattca aacaacgcta caataaattt tatggcgata aaggagagta tgaaaactct
    10681 tcattttctg aagaagatta ttttaaataa aacacaaaac gtcgatctta cgagaatagc
    10741 tcgtaggaac gacgttttgt gtttttgttt taatttttca gcaaagcaat tgcctggtat
    10801 ggttttagct ccagactttg atgaagaaca gtatcggaat aattatcgat aagaatattg
    10861 gcttctgtga atccttctgg gatggcgatc cttgtatctt tatcatagaa atttgttaat
    10921 acaagcagct gctgtccttc aaattggcga agaaagccat aaacttccgg atgatttgct
    10981 tcataagcat gataatctcc ttgtgcaacg attggatatg tttttctcag ccgaatcaat
    11041 ttcttgtaat aggacaaaat agaatcttca gaagatagtt ctttttctac attaatctct
    11101 tctagcttgc ctccaacttt taaccaaggt ttccctgtag taaatcctgc ttgttctgtg
    11161 tctgaccatt gcatgggtgt tcgagagtta tcgcgagatt ttgctttgat ccgacgaaac
    11221 gcttcttttt ctgtgaatcc ttgttcaagg agtaacttat aagcattcaa actttctacg
    11281 tcaatatagt cggaaatcga tgaatagtct gggtcgatca tgccgatttc ttctcccata
    11341 taaatatagg gcgtcccacg gttcaaatgg atcatggttg ctagcatctc tgctccttgt
    11401 tttcgatact gatcgatagc tacaaatcga ttgagcgcac gtggctgatc atgattatta
    11461 aaaaatagag cattccagcc gttaccctca ctcatctttt ctccccatgt atgaaatagt
    11521 cgctttaatt ctgcaaaatc aaatggagga gtagaccatt tattcccatt ttcataatcg
    11581 actttcaaat gatggaaatt aaagaccatt gataattctt ctctactagg ttctgtatac
    11641 aagatacaat tttcgatcgt cgtagagctc atttcaccga ctgtgatgat agtggaatcc
    11701 gtcccaaacg ttgcttggtt caattctttt agatacgtat gggtgattgg tttgtccgta
    11761 tattcaggtt tgccatcatt ttctttatta tttttcaatt gttcgtcttt accgataaca
    11821 ttgataacat cgaaacggaa tccttttacc cctttttcca tccaaaaacg aacaactttg
    11881 aatagttcct ctcttactgc ttggttccgc cagttcaagt cagcttgtgt ccgatcatat
    11941 aagtgcaaat aatatttccc cgtatctcca aatggcgacc aagcacttcc accaaatttt
    12001 gatacccaat ccgttggctc ttctcttaaa atgaagaagt cctgataatg ctggtcccct
    12061 gctagtgctt ttttgaacca ttcatgttct gtcgaaacgt gattcagcac catatcgagc
    12121 atgatttcta tccggtattt ctttgcttcg gctattagct gttcgaattc ctccatcgta
    12181 ccaaagatag gatcgatggc tgtgtaatca gagatatcat aaccgttgtc tttttgaggg
    12241 cttggataaa tagggtttag ccaaatcatg tcgatgccta gatcattcag atacgggagt
    12301 ttttctttga ttccttttaa atctccaatt ccatcaccat tactgtcttt gtaagatttg
    12361 ggatagattt ggtaaatgac tttgtcttta aatgtcatgg atatttacct ccattcaact
    12421 gatttattac ctaccacaac ttatatatac aagttatcat ttattattat aactgaaccg
    12481 aaagcgtttg taaaatttat ctttatacat tctaagaggt ctgttgccca ataaaaaaca
    12541 agagatcaga catgtatttg tctatcctct tgtttaaaat agcaaaatat tcttgtttct
    12601 tctataaatg gtgttcaaaa gcttttatct acggcaatca gtcttctcaa atgtaaaaca
    12661 tgaggagcta attttatttg agtattcttt ttgctcgatg caggacggct tttttacaac
    12721 ctctgattca tggtgttcaa ttagcagctg accctcgtta ttagctcaaa acgctgattt
    12781 cacaacctct gtttttattc attcgatttc aacgcatcaa tcatatcgat atgctttaat
    12841 ttgaaatggg taacaatcat cacaataacc gtaaatacga tagttaatcc tgctgataag
    12901 acataagccc accatgtgat cactaatgga aagatgacat tctccataga cgcttgttgc
    12961 aagatgaagt cagtcaatat atatcctaca ccaaatcctc cgatgattcc taaaagagta
    13021 aagatgatat tttctcgaac aatgtacatc gtgacttctt tatcaaaaaa tccaaggact
    13081 tttattgtag ataattcgcg tacacgctcg gaaatattga tattagttag attatacaag
    13141 acgataaaag ccagcagtcc tgataatacg acaaagatta agacaaccga gtctaagttc
    13201 gccattgatt cttcttgcgt ttcgatttgt ttggacatga aagacgtatt tgtcacagcg
    13261 tctgtggcaa gcagtttctc ggaaagcatt tcttcttctt ttgttgtcat cttatctgta
    13321 cgcagcaaaa atgcattatc catcatcgat tgatcagtaa ctgaagcata atattcaggg
    13381 gacatataaa taaagctacc tagatagttt ttcacgattt cttttacttc gactttccat
    13441 tctttttgat cttcatcata aagcgaaata tgatctcctg tagataagtc aaatagttct
    13501 gctacttttt gcgtaatagc gattcctgtt ttaggcaagg agcgtgggtt ctcttcaaga
    13561 tcagttaaat ggataaaacg atccaaatca gctgttttac ttggtacgat cactgtcaga
    13621 ctttgcgaag catgtccttt ttttctcaat tctacggttt gagtatgaat agctaggatc
    13681 tcatttactt tttcttgctt ctgcagcata tccgctgctt tttgacgact atcttccgtg
    13741 gatgaatcca atgtaacgat tgcttgatag tcaatgatcg gtccaaattg tttggctgaa
    13801 acagaactta gagaatcttt caatcccacg cctgcaacca tcagcccggc acatccagca
    13861 attccgataa tcgccataag cattcttgac ttatatcgga agagatttcg gtaactgatt
    13921 ttttgattaa agctaaggcg tgaccacagc ggagtgatgt attcaagaaa aatgcgtttc
    13981 cctggcttcg gtgctttcgg ttgaagcaat gcagcaggcc gttctcttaa atctttgaat
    14041 aaaacgatca ttgcagcgcc aaatgcggca atgaagaatg cggcagctgc ttgtaaaatc
    14101 ggcggccagt cataaaaaat aagcgcactc ccaatatcat accgctcgtt agacagctca
    14161 aaaatgatcc ttggcaaaag attcgttcct aatatcgttc ctaaaacaat acctatccct
    14221 gaggctagac ttgcatagat tgcatatttt tttgcgattt caactttcgt ataacctaac
    14281 gcctttaatg tcccgatttc cctgcggttt tcttctacca tacgtgtcat tgtcgtaaag
    14341 gtgatcaatg cggcaataaa gaaaaagaaa acaggaaaaa cattggcaat cgcagcgata
    14401 cgatcagaca gatcaccaaa ttcttgaaaa ccgacatttt ctgaacgttc attgtatagg
    14461 tatgatggtt tagttaaatt ttcaattttt tctgcatttc gattcaattc ttcctctgca
    14521 gaggccagtt cacgttcttt ctcaactaat tgcctttctt gttcagcaag ttgagcttct
    14581 tgttgagata atgtttgaac agcgattgtt cgttgcgctt caggaagctg ctggatagtt
    14641 gctgactgtt gcgctaactg ttttttagca tcttctaacc gtgttcttgc ttgcgttgtt
    14701 tgtgctttgc catccgctat tttttgctgt gccggttcta attcttcgtt ggccgatttt
    14761 ttcagctctt ctaagcgttc ttcgggtcga tcttgtaaat acttttctgc cttttcttga
    14821 ttttgctcca ttttatcttt gtaagctttg ctgtacgtat tcaaattctt tacgtttgta
    14881 aaatcaagat ataagacaga ataaatatct gttttaaaaa cggattcagg taaatagaca
    14941 aaataatcga cgctaccatt tccgacgttc gcgtatcctc tctccaaatt gttgatgaac
    15001 aatggagaac ggacaaagcc cgtgatggta tactcttttt ctttcaattc atctgaatca
    15061 atctgataaa tatcaccgat tttataccct tcatcttccg ctttttcatc caatacaagc
    15121 tggtttggtt tttccgggag ttttccatca gttaattgta gcttgttctg cttgttattt
    15181 ttgttataag aaaatatttg aatcacttcg tttttgccag tattggaata atagatctgt
    15241 tttcctgttt ctacttttgc atctgggatc ttttctgctt gagcaatatc tttttgtgtc
    15301 aagccaccag tagaaacgat ttgcaaatca gaaagtcctg ctgtaccaac atagtgatcc
    15361 atcgtttttt gcatgacagg tccagcagtt ttgacaccta caaataaaag tgttcctagc
    15421 atgacaataa ggatgattgc aatgaatcgt cctttagatt gccgaatttc gcgaaaacct
    15481 gctttaagat atgttcttgt tttcataaaa tccccctacc atacaatctc atcaatggat
    15541 gaaggatgat catttaattc aacagacctt actttagcat cattaatgtg aatgactcta
    15601 tcagcgatgg gcgctaaagc agaattatgt gtaatgatca aaacggtatt gccgtgttgt
    15661 ctgctagcgt tttgcaataa tttcaatact tgcttgcccg tttcaaaatc cagcgcgcct
    15721 gtcggttcat cacataaaag aagtttggga tttttagcta gtgcacgagc aattgaaacc
    15781 ctctgctgtt cgcctcctga taattgggac ggaaagttat tcaatcgatg agctaggccc
    15841 acttgcctca atacctctac aggatcaagc gcatcaggag aaacttccgt tgccaattcc
    15901 acattttctt ttgctgttag attaggaacg agattgtaga attggaaaac gaaccctaca
    15961 tctgttcgtc ggtaagcagt aagctgttta tctgaaaatt gcgcaatatc tgtgtcatca
    16021 ataatgatct gaccttcatc aggtgtatcc atcccaccta aaatattaag aatcgttgac
    16081 tttcctgctc cacttggacc aagtataatc actaattcgc ctttttcaat tgaaaaagaa
    16141 atatcgtcat ttgctgtaat ggtcgtttct cccattttgt atcgtttgta ttcatttttt
    16201 acttcgacat acttcactct gattcctcca ttttcacgat atttctcgct atatctatgc
    16261 atcgaatgcg ttttctcaca cttatttatt ttaccactga atttctctag actaaagaaa
    16321 ggaatccttt aatttagttg acaaataaac gatttatgat ttacttacat aaaaaacatt
    16381 gttatttgaa agaaggaaca tcatgaatga tttaaaggaa aatatgattt ttgatttatc
    16441 aaaaactgct tttgtcgcga tcgacatgca aaaagggatc gtaaataacg gtacactctc
    16501 tccacacact gcacaatcta ttctgacaaa caatgatttg ttagcaaaaa cattgaaacg
    16561 taccgctgcc ttgaaagttt tagtgaatgt agacatcact actttctctt atctaagtca
    16621 tcagacagat gctggaggaa tatctgcttt gtctgctccg gcagaattca ctgacttgtt
    16681 gttgaaggat tccttgaaag atacaaacaa tatgttgact atcacaaaat acaatccaag
    16741 tgcctttttt ggtacttctc ttgatcttca acttcgccgg cgtggaatcg aaactatcgt
    16801 tttaagcggt gtagcgacaa caaacggtgt atatgctact gcattagatg ccttccaaca
    16861 tgggtatcat gttgtacttg ctgaagatgc ttgcagtgat cgtgacaaag aatcccatca
    16921 attgttcata aaaaagatat ttccgaaaac tgcacgagta cgctcaacga aacaaataat
    16981 cgaagctatc caacaaagca aataagtact ggcacacgtg attttcattt acttcttttt
    17041 gatttcatgg gaaaataacc acaggattta gataggagta tgaacatggt gaaaaaaaag
    17101 atcacaatca aagaagtcgc taaactttcc ggcgtttcaa tcacaactgt ctccttgata
    17161 ttaaatggaa atacggagaa attcagtccg aaaactgttc agaaagtttt agctgccaaa
    17221 gaagaattag gctatcagcc tgactatttt gctagacaaa tgattaccaa ggaaacgaaa
    17281 acaattggtg tactcgttcc tgatatcacg aatccattct tcagtacact gatgcgagga
    17341 attgaggata tcttgtataa gcagaatttc gtcacgatat tatgtaatgc tgattctgat
    17401 catcaaaaag aaatcgaata tctagccgag ctcacacgtc gaggtgtgga tggattcatt
    17461 atcgcgactt ctgctgtctc tacagatgca atcaacgaaa atttgaaaaa acaaggccgg
    17521 cccttcattg ttttggatca gaaaaaatct gaaggattta gtgatgccgt acgaacagac
    17581 gattttcgcg gtggttacct agcgggcatg cacttgctta gtctaggtca tcaaacgatc
    17641 gcccttgtct atcctgaaaa tccgccggaa aatgtccacg ctcggattga ggggtttaaa
    17701 tctgctcttg atgtttacca aatctctcac gaccaactga tcctcttacc tacacagttc
    17761 tcaaaacaag gtggctacca aatcacagca gaacttcttg attctgctgc aactggtgtg
    17821 tttgctttaa atgatgaatt agcttttgga ttatatcgcg gattagagga atctggaaaa
    17881 agcattcctg aagactacag cattattggc tatgataata tagatatgtg cgagtatatc
    17941 aagccgaaac tgacaacgat cgcacaaccg atcttcgaat tgggacaaac atctgccaaa
    18001 ttattgttag atcggatcca attcccagaa aaagaatggg aagaaaaaag attacctgta
    18061 cgattagaaa aaagattttc aactgcaccc ttgaaatagc tttcaaggtg tgctatattt
    18121 ttatcgaact attaaaacgt tttactaaaa cgttttaaaa aataattttt cccatcaaaa
    18181 taaatgagat agttggaggt ttgtaaaatg aacacggtta cagtaattgg aagtattaat
    18241 atggacacta cccttcgttt aaccaacatg cccaagccag gcgaaacaat gcatgcgcat
    18301 gagatttttc acgctggcgg aggtaaaggt gccaatcaag cagtggcagc caaacgttca
    18361 ggcgcacgga catcttttat tggaggcgtt ggggctgaca gcgaaggaca acagttatta
    18421 gatctactga caaaagaaaa catcgataca tctgggatag ctgaaatcca aggcgccaca
    18481 accggacaag caatgattat ggtagacgca gcaggagaaa atagtatctt gatccatgca
    18541 ggtgcaaata atgctttcca tgaacaagaa gtcctaaaaa acaagcaact gattacaaac
    18601 agtgacttta ttattgctca gtttgaaagt agcttagatg cgacgatcct agctttttct
    18661 attgcaaaag acgcaggaaa aacaacgatc ctgaatcctg caccagctcg tgaaacgatc
    18721 cctactgaac tacttgaaaa aacagatatc attataccaa atgaaacaga aacagaaatc
    18781 attacaggaa tcagagtgac agatcataac agtttggtcg cagcagccga aaaactccat
    18841 gaattaggta ttggaacagt aatcattaca ctaggcagtg ccggcgcttt ctatcataca
    18901 gaaaaagaac acggtatcgt ccctgctttt aaagtggatg ccgttgacac aacagccgca
    18961 ggtgatacat ttattggcgc attaagcagc acacttcagc cagaccttag taacttgaaa
    19021 gaagcgatcc tttatggaaa tctagcctct tctgttgctg tgcagtctta tggtgcgcaa
    19081 ccttctatcc cttaccgcga agctttagaa caatcagcag tctaaaatta acaatgagag
    19141 gaagaaacaa acatgaaaaa aggaaaagta atcaattcag atatttcacg agtaatcgca
    19201 caaatggggc actttgataa attaagtatc ggggatgcag gcatgcctgt cccctttggt
    19261 actgaaaaaa tagatttggc tgtctcaaac ggtcttccaa gctttttatc tgttttagaa
    19321 aacgttttag aagaacttga ggtacaatcg attgaattgg cggaagaaat aaagacaatg
    19381 aacccggaaa tattggaaaa aatcaaatta ttgttaccag atacaccgat tagctttatc
    19441 ccacatgaag aaatgaaaaa agagttgaat aactgtcatg catttattcg tacaggcgaa
    19501 atgacacctt acgccaatat tatattagtg agtggagtgg tcttctaatg gaaacgacaa
    19561 tgaatcctga acagatggta ctgtccgagc agattcatat gctgattgaa cagttgggag
    19621 gaaaagacaa tattgattct gtccttccat gtgcgactcg gcttcgtatc aaattgaaaa
    19681 aacagccagt agccaatgca gaaaaattaa aacagcaaat cccttccatt aagtatttca
    19741 ttttactcga tagatatgca gaaataatca tggatcaggg attggctcct gcttacaagg
    19801 aattaacgcg tcaaatcgat cattcagctc ctaaagaaaa gctaaatccg atcgaaaaac
    19861 tatttggttt gatatcggaa atctttacgc cgatcattcc gctgtttgcc ggttctggta
    19921 tattaaaagg tctggttgtt ttagcgacta cgattggctg gcttagtgaa acaagcggaa
    19981 cttatcatat tttgtctgct gcgtcgaatg ccgtctttta ttttctgcca atctttcttg
    20041 ctttttctgc cgccaaaacg tttaaagtga atggatatat tgcggctgtc attgctgcag
    20101 cactgattga accgaatctg acctctttac tttcagacag tggcaaagca gctactgatt
    20161 ttctggggat tcctgtagta ctgatgcagt atacgtctac tgtcatgccg gcgattttag
    20221 gtattttctt ttattctttt gttgaaaaat tcctcaaaaa atatattcgc gaaaatatgc
    20281 aattagtatt tgtaccttta ctttcattag ttatcacggt tcctttgacg ttaatggtat
    20341 tcgggcctgc gggtgtttac cttggcgaag gtttagctgc cgcaattact tggatgatgg
    20401 atattaacgg accgatcagc ggcgcaatca tcgctggtgg ttggaatatt ttggtaatat
    20461 ttggtatcca atgggctgta aaccctgtca tgatcagtaa tatctctgct tatgggttcg
    20521 accgaatcgt tccgttgaca ggagcagcca attttggaat ggctggtgca gctttaggtg
    20581 tatttctacg aagtaaacgt tcaaaaactc gttcgatctc cggttctgct tttgcttcga
    20641 ttttacttgc gggagtgacc gaaccaactg tttacggaat tgcgatccca ttgaaaaaac
    20701 catttgttgc tgcttgcatt ggtgcagctg ctggtggagc tgtaatggga tttgcccaag
    20761 tgaaagcgat cgcttttgta tttggtagtc tgaccacatt gcctgcattc atttccggta
    20821 cttttttctg gtacttagca ggactggctg ttagtctagt cgtagcaatg attactactc
    20881 tcgttagcgg gtttgatgaa gacctaatgt cttatgaata gttaatgaaa aagactgaag
    20941 tatgagatgt ttatcacata tttcagtctt tttcttttta ggatcattat ttttataaat
    21001 ttttgtgata ctttccaaac tttggtataa tttacatatg cataaccatt tttatcccca
    21061 cttgtttatc cccaaacaaa ggtggtctat gcaataaaat gaacgtcaat gaaaaacgaa
    21121 tgaatgctcg ttgcttattt acaaattgtt ttatttagaa tacacagaca gatttatgaa
    21181 aagaaaaagc ttttcgacac gattatctgt tccaaaaaat gtctaatgaa tgctgaataa
    21241 gacagaaaat caatgaaagg aaaaggacta gtttattatg aaaaataaaa aaacgatacg
    21301 tttgctagct gctacttggc tgattgctcc gtctgttttg tcgaccaata ccgtatttgc
    21361 cgtagataat caaacagctg atcaaacagt cacaacagat acgactacat ccagctcagc
    21421 tactagtgaa acaacggatt cttttacaga agacccaact ggaacaacta actcaacaac
    21481 aggatcgacc gagccaactg aaattgatcc ggctgatcca tcagaaaatg aaaagccagc
    21541 cgagacatat tcccttacaa ttgctccttc tttgattggt aagatccaac tgactgcttc
    21601 gacagcggat tcccctgaac agcaaccttt ggatattacg gaaagtggaa cggtagaagg
    21661 tttattagcg gaaacgaagg taacttacac tattacgcca gctgaaggtg aacaaattgc
    21721 ttcagtcgct gttaatggtg ccaaagaagt aaattatgat gcaaagacct ttacagtagg
    21781 tacgaacaat ttatctatta ctgcagaatt tgaacctgta ccggatactt caacagagaa
    21841 accaactgat cctagtgaag aagtccctag tacaccagat ccggaaacgc caaacaaacc
    21901 agatgtgggt aataacaaca ataataatgg ttcaaataat tcaaacaata ataacaataa
    21961 ttcgaacagc gggaattctt cgaatcaaac aaacccatct ggatcgaata acaatggaaa
    22021 tactcagaga gaaccaaacc atccaaacaa tggttcatca aatggcggaa cgaacaatgc
    22081 ttcatctcgt ccaaatacag caaatcaaag cggtatagag aatccatctt ctgcttcttc
    22141 tgattttgtt gtaaaatcac caattgatgc tgtattgcct acaaatacaa cgaatgtcca
    22201 acaagcaatc gttcgagaag cattcaagca tcttggaaaa ccatatgtat ggggagcaaa
    22261 aggtccaaat acttttgact gttctggttt gacttattac gtatatatga aagcaaccgg
    22321 tcattatatc ggtggctgga caggcgaaca gcaatatgca ggtacacaga ttccagtaag
    22381 tcaggcccaa cctggtgatc tcgtcttctg gggaccttct tctggtgtta cccaccacgt
    22441 aggtatctat attgggaacg gacaatttat ccatgcgcct caaccaggag ataaagttcg
    22501 agtaacttct atttcagatt tcacgcctga ttttgctgtc cgagtaaact tagcaggttt
    22561 acctgctgct tcaggttcgc tgacaggcgg ctcatctatt ttagatggat tagatggctc
    22621 gttccatttc agtcaaaacc aaacaactga tcagttcttg aaaaagattg cagatgatgc
    22681 acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc atgatggctc aagctatcct
    22741 tgaaagtgga tctggaaaca gcttattatc aagcgaacca aatcataacc tttttggtat
    22801 caaaggatca tataaaggaa gcagcgtgac cttcaataca cttgaacaag atagttccgg
    22861 acaaagctac caaatccgcg cacaattccg taaatatcct tcttacaagg aaagtttgga
    22921 agactatgcc gacttgatca aaaatggact gactggcaat cctgatttct acaaaccaac
    22981 ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa tatttggaag gacgttatgc
    23041 tacagatcgc caatattcac aaaaattgaa cgcgatcatc gaagcgtacg acctgacaaa
    23101 atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa gcaaacgctg aaatcctcaa
    23161 tatgtctaag cgattcgatg tgccaatcaa atggacaaca gatggcgtgc caatcacagg
    23221 attagaattt tcaacgataa aacgcgtcaa caacgcttct tccatcttcc gtgtcgcttc
    23281 aatctgggat ttgtggaaca attttacggc aagacagata ccagaaacaa ctactcgtac
    23341 agttaaagct gcaactgttc ctgtactaac cttgttaagt ttagacaaat cattctggct
    23401 tgatctgcct ttaaagaaat agaataaatt aaaaagggga tgtgacactg tcacactccc
    23461 ttttta
//
LOCUS       DACCPY010000044        23450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCPY010000044 DACCPY010000000
VERSION     DACCPY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.045"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            150..446
                     /locus_tag="IVO69_001463"
     CDS             150..446
                     /locus_tag="IVO69_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400245.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            453..2405
                     /locus_tag="IVO69_001464"
     CDS             453..2405
                     /locus_tag="IVO69_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7400246.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="IVO69_001465"
     CDS             complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="IVO69_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7400247.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3336..4376
                     /locus_tag="IVO69_001466"
     CDS             3336..4376
                     /locus_tag="IVO69_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7400248.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4474..4662
                     /locus_tag="IVO69_001467"
     CDS             4474..4662
                     /locus_tag="IVO69_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7400249.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4720..5073)
                     /locus_tag="IVO69_001468"
     CDS             complement(4720..5073)
                     /locus_tag="IVO69_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400250.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5604..6026
                     /locus_tag="IVO69_001469"
     CDS             5604..6026
                     /locus_tag="IVO69_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7400251.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6023..6253
                     /locus_tag="IVO69_001470"
     CDS             6023..6253
                     /locus_tag="IVO69_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7400252.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6751..6951
                     /locus_tag="IVO69_001471"
     CDS             6751..6951
                     /locus_tag="IVO69_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7400253.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            6978..8171
                     /locus_tag="IVO69_001472"
     CDS             6978..8171
                     /locus_tag="IVO69_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7400254.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8239..9804)
                     /gene="guaA"
                     /locus_tag="IVO69_001473"
     CDS             complement(8239..9804)
                     /gene="guaA"
                     /locus_tag="IVO69_001473"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7400255.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9863..10786)
                     /locus_tag="IVO69_001474"
     CDS             complement(9863..10786)
                     /locus_tag="IVO69_001474"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7400256.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10982..11587
                     /locus_tag="IVO69_001475"
     CDS             10982..11587
                     /locus_tag="IVO69_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7400257.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11895..12911
                     /locus_tag="IVO69_001476"
     CDS             11895..12911
                     /locus_tag="IVO69_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400258.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13052..14353
                     /locus_tag="IVO69_001477"
     CDS             13052..14353
                     /locus_tag="IVO69_001477"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7400259.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14399..15061
                     /gene="deoC"
                     /locus_tag="IVO69_001478"
     CDS             14399..15061
                     /gene="deoC"
                     /locus_tag="IVO69_001478"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7400260.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15082..15480
                     /locus_tag="IVO69_001479"
     CDS             15082..15480
                     /locus_tag="IVO69_001479"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7400261.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15546..16628
                     /locus_tag="IVO69_001480"
     CDS             15546..16628
                     /locus_tag="IVO69_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7400262.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16840..18402
                     /locus_tag="IVO69_001481"
     CDS             16840..18402
                     /locus_tag="IVO69_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400263.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18395..19528
                     /locus_tag="IVO69_001482"
     CDS             18395..19528
                     /locus_tag="IVO69_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400264.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19528..20484
                     /locus_tag="IVO69_001483"
     CDS             19528..20484
                     /locus_tag="IVO69_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400265.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20583..21749
                     /gene="deoB"
                     /locus_tag="IVO69_001484"
     CDS             20583..21749
                     /gene="deoB"
                     /locus_tag="IVO69_001484"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7400266.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21773..22591
                     /locus_tag="IVO69_001485"
     CDS             21773..22591
                     /locus_tag="IVO69_001485"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7400267.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22608..23315
                     /gene="deoD"
                     /locus_tag="IVO69_001486"
     CDS             22608..23315
                     /gene="deoD"
                     /locus_tag="IVO69_001486"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7400268.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7616 a         4159 c         4902 g         6773 t
ORIGIN      
        1 gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat
       61 tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa
      121 aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat
      181 ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg
      241 ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg
      301 aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg
      361 ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa
      421 tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga
      481 aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg
      541 ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt
      601 tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca
      661 tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga
      721 aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact
      781 gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta
      841 attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg
      901 agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg
      961 agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta
     1021 aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc
     1081 aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca
     1141 gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc
     1201 tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga
     1261 aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta
     1321 agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata
     1381 tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt
     1441 ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca
     1501 atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg
     1561 ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga
     1621 gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt
     1681 taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta
     1741 ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt
     1801 ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag
     1861 caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga
     1921 ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta
     1981 ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag
     2041 ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac
     2101 caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc
     2161 aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata
     2221 agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc
     2281 taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac
     2341 ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata atttatttca
     2401 aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct
     2461 tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt
     2521 tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat
     2581 aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca
     2641 ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt
     2701 cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat
     2761 taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc
     2821 cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa
     2881 catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat
     2941 tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt
     3001 taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg
     3061 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
     3121 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
     3181 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga
     3241 actacttact tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
     3301 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
     3361 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
     3421 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
     3481 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
     3541 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
     3601 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
     3661 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
     3721 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
     3781 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
     3841 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
     3901 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
     3961 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
     4021 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
     4081 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
     4141 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
     4201 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
     4261 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     4321 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
     4381 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     4441 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     4501 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     4561 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     4621 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     4681 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     4741 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
     4801 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
     4861 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
     4921 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
     4981 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
     5041 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
     5101 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
     5161 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
     5221 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
     5281 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
     5341 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
     5401 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
     5461 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
     5521 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
     5581 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
     5641 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
     5701 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
     5761 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
     5821 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
     5881 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
     5941 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
     6001 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
     6061 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
     6121 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
     6181 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
     6241 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
     6301 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
     6361 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
     6421 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
     6481 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
     6541 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
     6601 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
     6661 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
     6721 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
     6781 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
     6841 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
     6901 gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac
     6961 agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt
     7021 attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg
     7081 gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag
     7141 aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc
     7201 ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta
     7261 agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc
     7321 aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag
     7381 gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat
     7441 ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag
     7501 aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta
     7561 tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat
     7621 gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga
     7681 atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat
     7741 tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc
     7801 agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg
     7861 ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc
     7921 attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc
     7981 taggacacga agatattgag acgactttag gaacgtatgg acatctttat cctaacagta
     8041 atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt
     8101 atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa
     8161 aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga
     8221 tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc
     8281 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     8341 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     8401 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     8461 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     8521 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     8581 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     8641 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     8701 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     8761 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     8821 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     8881 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     8941 atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     9001 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     9061 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     9121 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     9181 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     9241 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     9301 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     9361 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     9421 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     9481 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     9541 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     9601 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     9661 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     9721 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     9781 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     9841 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     9901 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     9961 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
    10021 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
    10081 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
    10141 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    10201 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    10261 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    10321 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    10381 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    10441 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    10501 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    10561 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    10621 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    10681 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    10741 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    10801 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    10861 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    10921 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    10981 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    11041 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    11101 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    11161 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    11221 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    11281 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    11341 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    11401 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    11461 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    11521 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    11581 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    11641 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    11701 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    11761 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    11821 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    11881 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    11941 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    12001 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    12061 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    12121 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    12181 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    12241 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    12301 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    12361 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    12421 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    12481 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    12541 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    12601 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    12661 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    12721 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    12781 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    12841 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    12901 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    12961 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    13021 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    13081 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    13141 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    13201 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    13261 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    13321 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    13381 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    13441 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    13501 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    13561 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    13621 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    13681 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    13741 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    13801 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    13861 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    13921 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    13981 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    14041 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    14101 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    14161 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    14221 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    14281 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    14341 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    14401 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    14461 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    14521 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    14581 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    14641 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    14701 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    14761 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    14821 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    14881 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    14941 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    15001 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    15061 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    15121 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    15181 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    15241 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    15301 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    15361 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    15421 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    15481 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    15541 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    15601 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    15661 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    15721 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    15781 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    15841 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    15901 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    15961 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    16021 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    16081 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    16141 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    16201 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    16261 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    16321 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    16381 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    16441 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    16501 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    16561 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    16621 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    16681 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    16741 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    16801 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    16861 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    16921 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    16981 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    17041 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    17101 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    17161 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    17221 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    17281 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    17341 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    17401 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    17461 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    17521 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    17581 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    17641 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    17701 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    17761 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    17821 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    17881 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    17941 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    18001 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    18061 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    18121 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    18181 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    18241 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    18301 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    18361 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    18421 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    18481 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    18541 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    18601 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    18661 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    18721 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    18781 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    18841 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    18901 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    18961 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    19021 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    19081 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    19141 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    19201 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    19261 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    19321 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    19381 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    19441 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    19501 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    19561 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    19621 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    19681 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    19741 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    19801 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    19861 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    19921 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    19981 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    20041 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    20101 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    20161 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    20221 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    20281 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    20341 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    20401 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    20461 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    20521 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    20581 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    20641 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    20701 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    20761 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    20821 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    20881 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    20941 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    21001 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    21061 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    21121 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    21181 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    21241 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    21301 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    21361 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    21421 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    21481 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    21541 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    21601 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    21661 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    21721 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    21781 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    21841 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    21901 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    21961 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    22021 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    22081 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    22141 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    22201 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    22261 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    22321 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    22381 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    22441 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    22501 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    22561 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    22621 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    22681 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    22741 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    22801 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    22861 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    22921 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    22981 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    23041 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    23101 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    23161 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    23221 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    23281 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    23341 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    23401 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
//
LOCUS       DACCPY010000045        23274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCPY010000045 DACCPY010000000
VERSION     DACCPY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.046"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IVO69_001487"
     CDS             156..1862
                     /locus_tag="IVO69_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7400269.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVO69_001488"
     CDS             complement(2020..2922)
                     /locus_tag="IVO69_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7400270.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVO69_001489"
     CDS             complement(2919..3623)
                     /locus_tag="IVO69_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7400271.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVO69_001490"
     CDS             complement(3654..4295)
                     /locus_tag="IVO69_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7400272.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IVO69_001491"
     CDS             complement(4333..4647)
                     /locus_tag="IVO69_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400273.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IVO69_001492"
     CDS             complement(4635..5264)
                     /locus_tag="IVO69_001492"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7400274.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVO69_001493"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVO69_001493"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7400275.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IVO69_001494"
     CDS             complement(5984..6916)
                     /locus_tag="IVO69_001494"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7400276.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IVO69_001495"
     CDS             complement(6913..7689)
                     /locus_tag="IVO69_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7400277.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVO69_001496"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVO69_001496"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7400278.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVO69_001497"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVO69_001497"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7400279.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IVO69_001498"
     CDS             complement(12014..13294)
                     /locus_tag="IVO69_001498"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7400280.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IVO69_001499"
     CDS             complement(13313..14239)
                     /locus_tag="IVO69_001499"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7400281.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IVO69_001500"
     CDS             complement(14252..15535)
                     /locus_tag="IVO69_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7400282.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVO69_001501"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVO69_001501"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7400283.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IVO69_001502"
     CDS             complement(16323..17234)
                     /locus_tag="IVO69_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7400284.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IVO69_001503"
     CDS             complement(17231..17695)
                     /locus_tag="IVO69_001503"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7400285.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IVO69_001504"
     CDS             complement(17700..18398)
                     /locus_tag="IVO69_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7400286.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IVO69_001505"
     CDS             complement(18385..18771)
                     /locus_tag="IVO69_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400287.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IVO69_001506"
     CDS             complement(18918..20588)
                     /locus_tag="IVO69_001506"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7400288.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IVO69_001507"
     CDS             20771..21004
                     /locus_tag="IVO69_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7400289.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IVO69_001508"
     CDS             complement(21103..21534)
                     /locus_tag="IVO69_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7400290.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IVO69_001509"
     CDS             21613..22026
                     /locus_tag="IVO69_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7400291.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IVO69_001510"
     CDS             complement(22064..23212)
                     /locus_tag="IVO69_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7400292.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT         6618 a         5162 c         4096 g         7398 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg tttt
//
LOCUS       DACCPY010000046        22385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCPY010000046 DACCPY010000000
VERSION     DACCPY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.047"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IVO69_001511"
     CDS             complement(218..1015)
                     /locus_tag="IVO69_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7400293.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVO69_001512"
     CDS             complement(1012..2196)
                     /locus_tag="IVO69_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7400294.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVO69_001513"
     CDS             complement(2221..3468)
                     /locus_tag="IVO69_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7400295.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVO69_001514"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVO69_001514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7400296.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVO69_001515"
     CDS             5448..5762
                     /locus_tag="IVO69_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7400297.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVO69_001516"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVO69_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7400298.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVO69_001517"
     CDS             6447..7091
                     /locus_tag="IVO69_001517"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7400299.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVO69_001518"
     CDS             7106..7435
                     /locus_tag="IVO69_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400300.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVO69_001519"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVO69_001519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7400301.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVO69_001520"
     CDS             8423..9247
                     /locus_tag="IVO69_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7400302.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVO69_001521"
     CDS             9240..9587
                     /locus_tag="IVO69_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7400303.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVO69_001522"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVO69_001522"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7400304.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVO69_001523"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVO69_001523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7400305.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVO69_001524"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVO69_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7400306.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVO69_001525"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVO69_001525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7400307.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVO69_001526"
     CDS             15244..16011
                     /locus_tag="IVO69_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400308.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVO69_001527"
     CDS             16001..18043
                     /locus_tag="IVO69_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400309.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18315..18464
                     /locus_tag="IVO69_001528"
     CDS             18315..18464
                     /locus_tag="IVO69_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7400310.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18475..19989
                     /gene="dltA"
                     /locus_tag="IVO69_001529"
     CDS             18475..19989
                     /gene="dltA"
                     /locus_tag="IVO69_001529"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7400311.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19986..21191
                     /gene="dltB"
                     /locus_tag="IVO69_001530"
     CDS             19986..21191
                     /gene="dltB"
                     /locus_tag="IVO69_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7400312.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21236..21469
                     /gene="dltC"
                     /locus_tag="IVO69_001531"
     CDS             21236..21469
                     /gene="dltC"
                     /locus_tag="IVO69_001531"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7400313.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21472..>22385
                     /gene="dltD"
                     /locus_tag="IVO69_001532"
     CDS             21472..>22385
                     /gene="dltD"
                     /locus_tag="IVO69_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7400314.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVL"
BASE COUNT         6992 a         4030 c         4639 g         6724 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg
    18181 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
    18241 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
    18301 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
    18361 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
    18421 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
    18481 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
    18541 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
    18601 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
    18661 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
    18721 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
    18781 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
    18841 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
    18901 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
    18961 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
    19021 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
    19081 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
    19141 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
    19201 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
    19261 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
    19321 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
    19381 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
    19441 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
    19501 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
    19561 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
    19621 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
    19681 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
    19741 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
    19801 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
    19861 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
    19921 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
    19981 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
    20041 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
    20101 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
    20161 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
    20221 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
    20281 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
    20341 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
    20401 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
    20461 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
    20521 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
    20581 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
    20641 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
    20701 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
    20761 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
    20821 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
    20881 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
    20941 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
    21001 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
    21061 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
    21121 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
    21181 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
    21241 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
    21301 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
    21361 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
    21421 gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
    21481 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
    21541 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
    21601 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
    21661 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
    21721 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
    21781 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
    21841 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
    21901 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
    21961 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
    22021 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
    22081 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
    22141 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
    22201 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
    22261 ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa
    22321 cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc
    22381 ttgga
//
LOCUS       DACCPY010000047        22240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCPY010000047 DACCPY010000000
VERSION     DACCPY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.048"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IVO69_001533"
     CDS             171..515
                     /locus_tag="IVO69_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7400315.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IVO69_001534"
     CDS             516..935
                     /locus_tag="IVO69_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7400316.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IVO69_001535"
     CDS             complement(1027..1377)
                     /locus_tag="IVO69_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400317.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IVO69_001536"
     CDS             complement(1343..2674)
                     /locus_tag="IVO69_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7400318.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IVO69_001537"
     CDS             2930..3496
                     /locus_tag="IVO69_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7400319.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IVO69_001538"
     tRNA            3641..3728
                     /locus_tag="IVO69_001538"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IVO69_001539"
     CDS             4062..5036
                     /locus_tag="IVO69_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7400320.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IVO69_001540"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IVO69_001540"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7400321.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IVO69_001541"
     CDS             complement(7451..7897)
                     /locus_tag="IVO69_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7400322.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IVO69_001542"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IVO69_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7400323.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IVO69_001543"
     CDS             8362..8880
                     /locus_tag="IVO69_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7400324.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IVO69_001544"
     CDS             complement(8957..9913)
                     /locus_tag="IVO69_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7400325.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IVO69_001545"
     CDS             10079..10885
                     /locus_tag="IVO69_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400326.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IVO69_001546"
     CDS             10882..11871
                     /locus_tag="IVO69_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7400327.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IVO69_001547"
     CDS             11868..12872
                     /locus_tag="IVO69_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7400328.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IVO69_001548"
     CDS             13004..13546
                     /locus_tag="IVO69_001548"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7400329.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IVO69_001549"
     CDS             13566..14264
                     /locus_tag="IVO69_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7400330.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IVO69_001550"
     CDS             14285..14497
                     /locus_tag="IVO69_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7400331.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IVO69_001551"
     CDS             14497..15459
                     /locus_tag="IVO69_001551"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7400332.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IVO69_001552"
     CDS             15477..15872
                     /locus_tag="IVO69_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7400333.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IVO69_001553"
     CDS             16035..16400
                     /locus_tag="IVO69_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7400334.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IVO69_001554"
     CDS             16397..18208
                     /locus_tag="IVO69_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7400335.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IVO69_001555"
     CDS             complement(18270..18437)
                     /locus_tag="IVO69_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7400336.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IVO69_001556"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IVO69_001556"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7400337.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IVO69_001557"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IVO69_001557"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7400338.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IVO69_001558"
     CDS             21194..22174
                     /locus_tag="IVO69_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7400339.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7212 a         3802 c         4630 g         6596 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagaggt tctgggttgg aaacctgaat acacagatat caaagcaatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc tccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 tgccgattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agcagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaaat
    20641 tgttgattga agcagctaac tcagtattgg aaaagtggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc aattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgtc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccgaca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacatt ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcgg tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggtttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tctctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt ggggaaatga
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa
//
LOCUS       DACCPY010000048        22225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCPY010000048 DACCPY010000000
VERSION     DACCPY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.049"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(39..1240)
                     /locus_tag="IVO69_001559"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IVO69_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IVO69_001560"
     CDS             1357..1872
                     /locus_tag="IVO69_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7400340.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IVO69_001561"
     CDS             complement(2071..2517)
                     /locus_tag="IVO69_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7400341.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVO69_001562"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVO69_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7400342.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IVO69_001563"
     CDS             complement(4396..5025)
                     /locus_tag="IVO69_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7400343.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IVO69_001564"
     CDS             complement(5009..5473)
                     /locus_tag="IVO69_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7400344.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IVO69_001565"
     CDS             complement(5960..7228)
                     /locus_tag="IVO69_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7400345.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IVO69_001566"
     CDS             complement(7459..8196)
                     /locus_tag="IVO69_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400346.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IVO69_001567"
     CDS             complement(8263..8832)
                     /locus_tag="IVO69_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7400347.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8960..9832
                     /locus_tag="IVO69_001568"
     CDS             8960..9832
                     /locus_tag="IVO69_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7400348.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10174..11046
                     /locus_tag="IVO69_001569"
     CDS             10174..11046
                     /locus_tag="IVO69_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400349.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11043..13001
                     /locus_tag="IVO69_001570"
     CDS             11043..13001
                     /locus_tag="IVO69_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400350.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13252..14103)
                     /locus_tag="IVO69_001571"
     CDS             complement(13252..14103)
                     /locus_tag="IVO69_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400351.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14226..14693
                     /locus_tag="IVO69_001572"
     CDS             14226..14693
                     /locus_tag="IVO69_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7400352.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14956..15438)
                     /locus_tag="IVO69_001573"
     CDS             complement(14956..15438)
                     /locus_tag="IVO69_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7400353.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQEVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15538..16401)
                     /locus_tag="IVO69_001574"
     CDS             complement(15538..16401)
                     /locus_tag="IVO69_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7400354.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16999..17847
                     /locus_tag="IVO69_001575"
     CDS             16999..17847
                     /locus_tag="IVO69_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400355.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18018..18359)
                     /locus_tag="IVO69_001576"
     CDS             complement(18018..18359)
                     /locus_tag="IVO69_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7400356.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18445..19152
                     /locus_tag="IVO69_001577"
     CDS             18445..19152
                     /locus_tag="IVO69_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7400357.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19139..19462
                     /locus_tag="IVO69_001578"
     CDS             19139..19462
                     /locus_tag="IVO69_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7400358.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19551..20588)
                     /locus_tag="IVO69_001579"
     CDS             complement(19551..20588)
                     /locus_tag="IVO69_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7400359.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(21035..22018)
                     /locus_tag="IVO69_001580"
     CDS             complement(21035..22018)
                     /locus_tag="IVO69_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7400360.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         6598 a         4610 c         3964 g         7053 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
     9001 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
     9061 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg gctcccgaga
     9121 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
     9181 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
     9241 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
     9301 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
     9361 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
     9421 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc gaaatcatcc
     9481 tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
     9541 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
     9601 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
     9661 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aaatattcat
     9721 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
     9781 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
     9841 tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat ccaacgatta
     9901 aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc agtaaattac
     9961 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    10021 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    10081 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    10141 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    10201 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    10261 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    10321 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    10381 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    10441 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    10501 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    10561 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    10621 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    10681 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    10741 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    10801 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    10861 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    10921 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    10981 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    11041 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    11101 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    11161 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    11221 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    11281 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    11341 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    11401 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    11461 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    11521 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    11581 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    11641 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    11701 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    11761 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    11821 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ctagcagcag
    11881 cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt atgttcgttg
    11941 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    12001 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    12061 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    12121 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    12181 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    12241 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    12301 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    12361 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    12421 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    12481 cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga
    12541 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    12601 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    12661 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    12721 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    12781 taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca agaggtcaat
    12841 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    12901 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    12961 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    13021 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    13081 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa cgaaacatat
    13141 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct ttttcacaaa
    13201 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    13261 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    13321 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    13381 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    13441 acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct tggctaattc
    13501 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    13561 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    13621 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    13681 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    13741 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    13801 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    13861 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    13921 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    13981 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    14041 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    14101 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    14161 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    14221 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    14281 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    14341 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    14401 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    14461 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    14521 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    14581 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    14641 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    14701 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    14761 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    14821 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    14881 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    14941 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    15001 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    15061 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    15121 cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga tgattcgcgt
    15181 agtgtttgga acctcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    15241 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    15301 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    15361 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    15421 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    15481 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    15541 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    15601 ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag gaagccattc
    15661 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    15721 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    15781 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc ggtctccatc
    15841 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    15901 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    15961 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    16021 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    16081 tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat cacgaaaagc
    16141 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    16201 tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    16261 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    16321 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    16381 ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta tcatattatc
    16441 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    16501 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    16561 atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa gtaaacaaag
    16621 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    16681 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    16741 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    16801 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    16861 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    16921 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    16981 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    17041 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    17101 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    17161 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    17221 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    17281 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    17341 cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    17401 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    17461 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    17521 gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    17581 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    17641 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    17701 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    17761 tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    17821 atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    17881 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    17941 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    18001 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg atagattttc
    18061 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccgctgca
    18121 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ccctaaccct
    18181 agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccatacttaa acaatagacc
    18241 gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc gcctcctccc
    18301 accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa tcccaccatt
    18361 gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta tgaaaattta
    18421 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
    18481 gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg tatcgttggg
    18541 aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt ctatgctggt
    18601 tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct ttccatcgtt
    18661 ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
    18721 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
    18781 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
    18841 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
    18901 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
    18961 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
    19021 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagc
    19081 aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
    19141 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
    19201 tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt tgcttgagta
    19261 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
    19321 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
    19381 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa ttctttccct
    19441 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
    19501 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
    19561 aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc tttggttgct
    19621 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
    19681 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
    19741 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
    19801 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
    19861 cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
    19921 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
    19981 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
    20041 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccggatccg
    20101 ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag accaaatttt
    20161 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc ttcttcatct
    20221 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta ttcttgattg
    20281 cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
    20341 acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat acgtaaagca
    20401 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
    20461 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
    20521 ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat taattccttt
    20581 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gtgttcattt
    20641 caatatatat agtatgctat ttgttccacc ttttttattc atcataatta ttctcttact
    20701 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
    20761 aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa atattcatca
    20821 aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
    20881 caacctctga tataaacggt gttcaatcaa ctgaccctcg gtattagctc aaaaaacgac
    20941 aaaatatgaa gagctatttt tgtttggctg ttcttattac ttgatacagg acagcttttt
    21001 cacgacctct aattcacggt gttcaagcaa gaaatcattg ttcactgaaa agcgttggat
    21061 gttctttttc aaaggtctct aagatctctc gtacttcatc agtagatttc gtattcatca
    21121 actgaactcg caagtcattt gctcctggaa atcctttgat atagatttta aagaaacgat
    21181 gtagtcctgt gatggaacgc gggatgattt ctgaataatg atcttgcaga tctagttgca
    21241 gacgaagtag tccgatcagt tctttttctg tatgttccct cgggtctttt tcaaatgcat
    21301 aaggattctt gaaaatccct cggccaatca tgatgccatc aacgccatat ttctcggcca
    21361 attcttctcc tgtctggcga tcggggatat ctccattgat cgtgattagt gtttgtggag
    21421 cgatttggtc acgtaaagca atgattttag gaatgacttc ccaatgtgca tctactttac
    21481 tcatctcttt acgcgtacgc aaatggacag aaagattggc tatatcttga tgaagtaggt
    21541 gactgatcca ttcttccatc tcagacattt ccgtatagcc gatacgcgtc ttgacgctga
    21601 caggcaaacc gccagcttta gctgcttcaa tcaattgtgc cgctacttct ggacgaagga
    21661 tcaagccact gccttttcca cgttctgcta cattagggac tgggcagccc atgttgatat
    21721 cgattccttg gaagcccatt tctgccatag caatgctcat ttctctaaaa aattctggct
    21781 tgtctcccca aatatgggca accatcggct gttcatcttc tgtaaatgtt aatcttcctc
    21841 gtacactgtc ttttccatct ggatgacaga aactgtctga gtttgtaaac tcagtaaaga
    21901 agacatccgg tgctgctgca tgctttacga catggcgaaa tacaacatct gttacatctt
    21961 ccattggtgc taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta gtagtcattt
    22021 atttgttact ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca
    22081 ttatactagg aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt tctgattgag
    22141 caaaaaaaag actgtgacaa aaatcttatc acagtccatt ttccggaata aatggtgttc
    22201 aaaagcttgc atttgcggta ataag
//
LOCUS       DACCPY010000049        21637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCPY010000049 DACCPY010000000
VERSION     DACCPY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.050"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(34..234)
                     /locus_tag="IVO69_001581"
     CDS             complement(34..234)
                     /locus_tag="IVO69_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7400361.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(539..1771)
                     /locus_tag="IVO69_001582"
     CDS             complement(539..1771)
                     /locus_tag="IVO69_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7400362.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2025..2594
                     /locus_tag="IVO69_001583"
     CDS             2025..2594
                     /locus_tag="IVO69_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7400363.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2772..3212)
                     /locus_tag="IVO69_001584"
     CDS             complement(2772..3212)
                     /locus_tag="IVO69_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7400364.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3370..4134
                     /gene="map"
                     /locus_tag="IVO69_001585"
     CDS             3370..4134
                     /gene="map"
                     /locus_tag="IVO69_001585"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7400365.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4166..5089
                     /locus_tag="IVO69_001586"
     CDS             4166..5089
                     /locus_tag="IVO69_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7400366.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5165..6304
                     /locus_tag="IVO69_001587"
     CDS             5165..6304
                     /locus_tag="IVO69_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7400367.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQLILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6297..7097
                     /locus_tag="IVO69_001588"
     CDS             6297..7097
                     /locus_tag="IVO69_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400368.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7097..7924
                     /locus_tag="IVO69_001589"
     CDS             7097..7924
                     /locus_tag="IVO69_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400369.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7902..8636
                     /locus_tag="IVO69_001590"
     CDS             7902..8636
                     /locus_tag="IVO69_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7400370.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8736..9602
                     /gene="rfbA"
                     /locus_tag="IVO69_001591"
     CDS             8736..9602
                     /gene="rfbA"
                     /locus_tag="IVO69_001591"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7400371.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9616..10188
                     /gene="rfbC"
                     /locus_tag="IVO69_001592"
     CDS             9616..10188
                     /gene="rfbC"
                     /locus_tag="IVO69_001592"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7400372.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10210..11238
                     /gene="rfbB"
                     /locus_tag="IVO69_001593"
     CDS             10210..11238
                     /gene="rfbB"
                     /locus_tag="IVO69_001593"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7400373.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11336..12187
                     /gene="rfbD"
                     /locus_tag="IVO69_001594"
     CDS             11336..12187
                     /gene="rfbD"
                     /locus_tag="IVO69_001594"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7400374.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12222..14255
                     /locus_tag="IVO69_001595"
     CDS             12222..14255
                     /locus_tag="IVO69_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400375.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14299..15579
                     /locus_tag="IVO69_001596"
     CDS             14299..15579
                     /locus_tag="IVO69_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7400376.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15789..16595
                     /locus_tag="IVO69_001597"
     CDS             15789..16595
                     /locus_tag="IVO69_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400377.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16607..17827
                     /locus_tag="IVO69_001598"
     CDS             16607..17827
                     /locus_tag="IVO69_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400378.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17817..19403
                     /locus_tag="IVO69_001599"
     CDS             17817..19403
                     /locus_tag="IVO69_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7400379.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19442..21580
                     /locus_tag="IVO69_001600"
     CDS             19442..21580
                     /locus_tag="IVO69_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7400380.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6800 a         3667 c         4511 g         6659 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc
      541 aagcccaatc gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga
      601 tatccatctt gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag
      661 cttctgctaa ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag
      721 aacctagtgc taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc
      781 cagattgaga aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac
      841 cttcatctgt atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa
      901 caacttttcc atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca
      961 gtggctttgt acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt
     1021 cttccgtcgg catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt
     1081 cccaaagatg gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa
     1141 gggcagaaaa ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct
     1201 tcttccatgc taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc
     1261 ataatgcctc aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt
     1321 taccagccgc agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat
     1381 tcattagtgc tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag
     1441 caaatgcatc gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt
     1501 catccgcttg atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta
     1561 aaagaaattc tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta
     1621 gtttataagc ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta
     1681 aaacaactgt gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt
     1741 tttttagaag ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct
     1801 ggtaactatt agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa
     1861 aaggaggtta tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc
     1921 ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa
     1981 tcaatggtgg aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga
     2041 cgattactag agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta
     2101 cacgatttac tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc
     2161 ttgagtttaa aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt
     2221 tacaatatga agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta
     2281 attatatcca ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat
     2341 atggtggagg aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac
     2401 gcgatccagc ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat
     2461 ggatcatagc tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg
     2521 aacagattga aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa
     2581 tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc
     2641 aaaataagga aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct
     2701 tccatgaatg caatgaataa aagagccata cagattgaat aaatctggat ggctctttta
     2761 ttgcaagtat attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata
     2821 tcttcttctt cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt
     2881 tttgcaaaag cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca
     2941 ccagatccgc agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa
     3001 aagtctacga tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa
     3061 atatctgcct cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg
     3121 atatccagat tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc
     3181 atgctagcat atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta
     3241 acaattatga attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg
     3301 gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga
     3361 gacgaaaata tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga
     3421 gctttattag cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt
     3481 tgggatattg aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa
     3541 attggctttg aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat
     3601 ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc
     3661 gatttgaaag gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct
     3721 gagatcgacc gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca
     3781 caagtaggga atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa
     3841 aatctagcga tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa
     3901 ccagcagttc ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt
     3961 atcacgatcg aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc
     4021 tggactgcgt atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt
     4081 actaaagatg gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc
     4141 cagtcaatag gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa
     4201 tctgatgcgc tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac
     4261 ctctgtcgtc gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg
     4321 taatatttta ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt
     4381 gattcctgag accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag
     4441 ttcaggaggc ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat
     4501 caatgctttg caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt
     4561 gatcgtccgt ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt
     4621 aactatcgtt cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat
     4681 acccgtgaac tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt
     4741 atttgtcatc atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac
     4801 gatcattccg ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag
     4861 tatctatgct aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat
     4921 tgtactcatg ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa
     4981 tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg
     5041 aaaatggttc aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt
     5101 cttattgtaa atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc
     5161 ccatatgctt gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact
     5221 gatattaaca cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa
     5281 tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc
     5341 atttgttata tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc
     5401 gatcttgatt gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct
     5461 gacacctaaa caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc
     5521 agatatacgt attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct
     5581 tagttttccc gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga
     5641 cggtttggat gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat
     5701 tggatatttt ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt
     5761 agcaagtatt atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga
     5821 tacaggagct ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa
     5881 cgtgactttt gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac
     5941 gttttttgct atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa
     6001 acatcttcat caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat
     6061 ttatgcgatg gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat
     6121 cggaacgatc atattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat
     6181 tggtctgatc aatgagaaac atcagttaat tttaaaaaca ttgcgatttt tagggaataa
     6241 agagtaccgt acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga
     6301 ataacaaatt aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt
     6361 ttcggacact agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa
     6421 tttttgacaa caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc
     6481 cctacatcga tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca
     6541 atctgatagc tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg
     6601 aagagacagt tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag
     6661 cacctaagat tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg
     6721 tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac
     6781 gactggctgt gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga
     6841 tctcaggtag ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg
     6901 agcgtttctt catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt
     6961 acaagttatt gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc
     7021 gaaatgcaaa actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg
     7081 ggtggcgttt tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact
     7141 attcagtcaa tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca
     7201 gcttattatt tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa
     7261 tatcgtttat catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt
     7321 gtctcaagca gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt
     7381 cgacgattat tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt
     7441 tccgatgatt ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa
     7501 tcgtacagct caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa
     7561 ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt
     7621 ttctttagat tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt
     7681 ggagatcctt cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc
     7741 aagtaatcga taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca
     7801 aaggcattta tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac
     7861 cgtcaaaaac cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt
     7921 gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc
     7981 agttagatag tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg
     8041 ggtcagtaga tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc
     8101 agcttttttc ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga
     8161 cgaaaggaga tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga
     8221 aaaagatcac tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa
     8281 tcattgtgga cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca
     8341 aagaaggatg gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc
     8401 ggtcttcttt aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt
     8461 ggattggttt gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc
     8521 gaagacatga tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt
     8581 cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa
     8641 agatgtttaa atccttaata tttccatgac aaatataaca attgatgaga taataacaaa
     8701 gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg
     8761 gcagcggaac acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt
     8821 atgacaaacc aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa
     8881 ttctgattat ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa
     8941 atgatttagg actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag
     9001 cgtttatcat tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca
     9061 atatttacta tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag
     9121 gcgcgacagt atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg
     9181 atgagaatat gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg
     9241 ctgtaactgg cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac
     9301 cttcagaacg tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa
     9361 aactatccgt agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat
     9421 cattgcttga agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg
     9481 cttgtttgga agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc
     9541 ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat
     9601 aaggagtcct tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa
     9661 atggacgtct ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt
     9721 gcagaacatg gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct
     9781 ggtgtccttc gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg
     9841 gtagtcactg gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat
     9901 ggtcaatggg acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa
     9961 ggatatgccc atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat
    10021 aattactata atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt
    10081 gattggccaa ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca
    10141 ttaaaagaat ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga
    10201 aattttctta tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc
    10261 gttcattacg tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact
    10321 tatgcaggaa acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta
    10381 ggagatgttg cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt
    10441 cattacgcgg cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa
    10501 acgaatatca ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat
    10561 catcacgtat ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga
    10621 catggggaag gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca
    10681 tattcttcta caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc
    10741 ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa
    10801 tttattcctc gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa
    10861 ggaaagaatg ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt
    10921 ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat
    10981 aagaccgtga tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat
    11041 gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa
    11101 gagctaggtt ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa
    11161 tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat
    11221 gccaaaaatg gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga
    11281 ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt
    11341 tttattaact ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa
    11401 aaatctgcca tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac
    11461 aatggcttat attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc
    11521 agtagataaa gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac
    11581 aagaaatgta gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga
    11641 ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc
    11701 actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga
    11761 agactattat atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt
    11821 cacgatgcaa cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg
    11881 gcgaccaact tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc
    11941 accatttggt atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa
    12001 agaaatattg aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca
    12061 aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt
    12121 tgtcatacca acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa
    12181 taagtaattt cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa
    12241 gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc
    12301 tgtttctaaa actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta
    12361 atttaaacgg aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga
    12421 aaaaaactga tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc
    12481 agacaccttt ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt
    12541 cattaaacgg acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta
    12601 tcggaaaacc attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt
    12661 attgggcgat gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa
    12721 cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag
    12781 tccagtggtg gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga
    12841 ttgcttttta tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc
    12901 ttttggtggt tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga
    12961 tgctggcgta cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag
    13021 tttcatggga tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag
    13081 tcatggttca tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat
    13141 atccaggaaa acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc
    13201 ttacaaattg gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc
    13261 tgttctatca cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa
    13321 aagatagcga acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct
    13381 tttggattac cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc
    13441 ctccattaag agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta
    13501 tcgcttatat ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag
    13561 taaatgcatg gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg
    13621 attttgaaac catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg
    13681 gctggagacg cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg
    13741 tcaatccagt cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc
    13801 aaaaaatcga tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact
    13861 tcaccccaac attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg
    13921 catggaagaa aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata
    13981 caagagcttt tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt
    14041 cggtcacgtt gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac
    14101 gaccattaac tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg
    14161 ataaagacgg ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa
    14221 cacaaactca ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa
    14281 atattagtag aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa
    14341 atgagtgtcg gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg
    14401 gtaggttttc cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt
    14461 gtggctattt tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta
    14521 ggtgttgcgc ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct
    14581 ttatttggag acttactgtt tcctttgatt attttatttt gtttagtaat taaatggatc
    14641 gagtggacaa aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc
    14701 acgttatatc gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca
    14761 ggcaattcgc tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact
    14821 cttgctttat tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc
    14881 ggtgggatag cagtcgttgc agccttttta ggtatttggg tctgccaatc tagaggtgca
    14941 ttagtcgcgt tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc
    15001 attcgtgcgc catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat
    15061 ctggcggctg tctctgaaaa agtcaatttg tttaccggtc gagaagatat ttggcataaa
    15121 ttctatcaga cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc
    15181 ttccagcgag gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc
    15241 tatggattga taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta
    15301 acgttgaaag cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg
    15361 atgatgcagt catttatgga ggatacattg acttcatacc catgggttcc gattatttat
    15421 atcctgttgg ctatggccgt tcatcgatat gatgaaccag aagttacgga ggaattctat
    15481 gttatggaag aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca
    15541 gatagcgctc attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag
    15601 gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa
    15661 atagaaacgt tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc
    15721 tcggtaccaa aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag
    15781 gaacacaaat gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat
    15841 tactgctaca gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa
    15901 tcttatgggc gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg
    15961 ttggactgaa agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg
    16021 ggatgattcc ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg
    16081 aatatactta tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa
    16141 tcctatccaa tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta
    16201 taaatggtat atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt
    16261 gtgctttctt gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata
    16321 tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga
    16381 gccctaatca gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg
    16441 ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat
    16501 atactttgta tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc
    16561 gtagattgaa accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc
    16621 aattagacta aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt
    16681 tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa
    16741 cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg
    16801 aaaatctaca atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga
    16861 aatcgaagga aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc
    16921 tggctatgaa aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga
    16981 tgaaatggtc gatgatatca ttgaatttgc tgacatcggc gaccatattt atcaacctgt
    17041 aaaaacctat tcaagtggga tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa
    17101 tcctgatatt ttgattgtgg atgaagcatt ggcagtagga gatcttgaat ttcaactaaa
    17161 atgtatggaa aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca
    17221 tgatattagt tcgattcgcc gtttttgtga tcgttcctat tggttgaaaa atggaaaagt
    17281 tgtcgaatat ggtgatacga tggaagtaac gacgaattac gaaaactttt tgaagaaaaa
    17341 atcagttaaa acagtagatc gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat
    17401 cgtagacgtg acgaaagccg aattgttgga taaagataaa aatccagtgg atatgctgga
    17461 acaaggagaa aaactatacg ttaaagtaac ctatgaagta aaagacgata caatcaaaaa
    17521 tccagtttta ggaattgctc tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac
    17581 tctactagat ggtatcaaga tcccttggaa aaaaggtgag aatatctttt atctggagta
    17641 tgaaaagtta ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa
    17701 tgcaacggtt cctttggtat acaagacaaa atacatgaac ttgtttgtca gcggttctta
    17761 tatcggagaa ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga
    17821 aatatgattt tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga
    17881 tcaaaccagg aagtaaagtt ttagagtttg gtcctggaaa cggccgatta acgaaacatt
    17941 tgatcggagc aaaacaatgt gaagtgagca ttgtcgagct agataaggaa ttgttcgatt
    18001 ttgtcagcga gtttgcccaa gatggatttt atggagatat cgaaagtttt gaatgggcaa
    18061 actattatgc tggacaaaca tttgattacg ttttgtttgc cgatgtttta gaacatttgg
    18121 tagatcctgg aaaaacgttg aagaaagtta gagaattttt gaatgaagaa ggagaaattc
    18181 taattacatt ccctaacctt gcgcataatt ctgtcatgat tgatttgttc aataatcaat
    18241 tgccatgggc ttcttatggt ttgctagatg aaacccataa ttctttttat actcatgatg
    18301 gcttccaaaa agtttttgaa aaagcaggat tatacatcaa tattgaagat tatttgtact
    18361 tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt
    18421 atgatttcaa aatgcgacca tttggcgaag tctaccaata cttcttctca ttgatgaaac
    18481 atccagtagc acaaagttcc attgcgcagc cgcaaaattc taattatgtg aaagtattag
    18541 aagtaatcca gcaaacgaaa caagatgaaa taattcaaca aataccattc aataatttca
    18601 caggggaaaa tgagacacta agtttccatg tatcagaatt agcagaacga atggtgtttc
    18661 gcttcgcgca gcaacctagc tttatcgaat tcagcgcaga agctgctgga gaaaaactca
    18721 catttatcga ttcaaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggta
    18781 aagatttacc agagttcttt ttaacggata ttgcagggaa agaagtgact atccattgtc
    18841 actatcgttt tatcggagaa ttgacaccaa caatgaaaga attgctggaa acaatccgtc
    18901 caatggctga agttacaaag cagctttctg agaaaaatga tgaattagaa agagaaaatc
    18961 attatcttat tgagaataat acaagattag atcagacgtt gaaaacaacc ataaatcgct
    19021 attgcacgtt gttggaaagt gatgaatgga caatcaaaca tagattaggg cgacgtaaga
    19081 aagaaacatc gaaaaaaatc caagaaaaag agctttctct atgtattgac gaaaaaatct
    19141 gggatgcaga aacaaagata ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc
    19201 aaccattgtc ctataagtta tcagccgacc aatcaccatt ttttgaagca attcctgttt
    19261 tacgagaaga agtcaatcaa gcaaaacagc tagctgaagg agcaaaagca ggatttgaac
    19321 tgcggatcct ttgcgagaag gaacgttcat tcttagttga agcagtggct caaaatggtc
    19381 aatcatggat cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat
    19441 cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg
    19501 cgatttgctt attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgataaa
    19561 ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc gtaagattgg atatcaatca
    19621 tttatataat cttgatgtta agacccaaag cggttttaag gtacgtttat cagggaaaat
    19681 gagaggaaaa gcaatcttag acttccaaac agcaagacat caaaatggca tagctgtgaa
    19741 attaaacggt aaatatcctt atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag
    19801 attactgaaa aaagggataa attatgcaag gactcatggc gtaaaaaaag caatccgccg
    19861 ggtcaaactg gaattgaaac caggatcgat cgactatgca gagtggatca gtcgtcatga
    19921 gaaacctgat ctgaaagagc aaaggaaaga agtacaagga tttgcctacc gcccattgat
    19981 ttccatactt atgcctgtct ataacgttga aatcaaatgg ctcgaaaaat gcattgattc
    20041 ggtacttgat caaacgtatg atcactggga actatgtatc agtgatgatg cttctactga
    20101 tccagctatc cgaaagtgtc tagaatccta tcaagcaaaa gatgatagaa ttaaagtcgt
    20161 tttccgacaa gaaaatggac acatcagtct ggcaactaat tctgcattgg aaatggcaga
    20221 aggagagttt atagcgctct tagataatga tgacgaatta cctccatttg ccttatatga
    20281 agtggcaaaa gtgctaaacg tacatccaga actggatttg atctacagtg acgaagataa
    20341 aatcgatgca gatgggaatc gctttgaccc gcacttcaaa gcagactggt ctcccgatac
    20401 attgatggga aataattata tctctcactt aggcgtatat cggacaagca ttgttaagga
    20461 acttggcggt ttccgtaaag gatatgaagg ctctcaagat tatgatttgg ttcttcgtgt
    20521 gacagaacaa attcctgcag aacacattta ccacattgac cgtgtcttgt atcattggcg
    20581 gacgatcccg ggatcaactg caagcaatgg agaagctaaa agctacatct atgattctgg
    20641 agtgaaagca ttgacagatg ctctatctag aagaaatatc aagggaagcg tacatcctgg
    20701 cagaatttct ggtttttatg aaatcactta tgatgttttg caagaagact tggtcagtgt
    20761 gatcattcca actaagaatg gctatgaaga tctaaaaact tgtgtggatt cgatcatcga
    20821 aaaaacgtct tatccaaatt atgaaatcat tattgcagat aatggaagta cagatcctaa
    20881 aatgcaagaa ttatttgcag aatacaagca tcagctgaaa gaccgattta tcgtcgaatt
    20941 aattgacatt ccattcaatt attcacgtat caacaatctg gcagcagaaa aagcaaacgg
    21001 caaatacttt ttattcttga ataacgatac agaagtgatt gaacctgact ggatgacagc
    21061 gatggtttct tatgcacagt ttgatcgaat cggctgtgta ggagcaaaat tattttatcc
    21121 ggatgataca acgcagcatg caggtgtatt attagggatc ggtggcgttg caggacatgc
    21181 attgaataac tatgatcgaa cacattgtgg ttactttggc cgattggtca ttgacgtcaa
    21241 ttacttagca gtcactgcag cttgtatgat ggtcaaggca gctgacttca atgctgtcaa
    21301 tggatttgat gaaatattag aagtagcatt caacgatgtt gacttgtgct tgaaagttta
    21361 tgaactgggt cgttacaatg tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa
    21421 atcaagaggt tatgaagata caccagaaaa acaaagacgt tttgctgggg aaatcaaaaa
    21481 aatgcaagat aagtggcctt cttatattgc tcatgaccca ttttataacg ataatctaac
    21541 taaagaagga atcggcgatt tttcgttgag acctgattga ttaaaagaac tgtattttca
    21601 tttttattaa tgaaaataca gttcttttaa tttaatt
//
LOCUS       DACCPY010000050        20910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCPY010000050 DACCPY010000000
VERSION     DACCPY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.051"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..303
                     /locus_tag="IVO69_001601"
     CDS             <1..303
                     /locus_tag="IVO69_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7400381.1"
                     /translation="IDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKE
                     NRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(727..897)
                     /locus_tag="IVO69_001602"
     CDS             complement(727..897)
                     /locus_tag="IVO69_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7400382.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1091..1270)
                     /locus_tag="IVO69_001603"
     CDS             complement(1091..1270)
                     /locus_tag="IVO69_001603"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400383.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1280..1504)
                     /locus_tag="IVO69_001604"
     CDS             complement(1280..1504)
                     /locus_tag="IVO69_001604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400384.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1657..2769
                     /locus_tag="IVO69_001605"
     CDS             1657..2769
                     /locus_tag="IVO69_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7400385.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2847..3140)
                     /locus_tag="IVO69_001606"
     CDS             complement(2847..3140)
                     /locus_tag="IVO69_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7400386.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3308..3622
                     /locus_tag="IVO69_001607"
     CDS             3308..3622
                     /locus_tag="IVO69_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7400387.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3643..5622
                     /locus_tag="IVO69_001608"
     CDS             3643..5622
                     /locus_tag="IVO69_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7400388.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5635..5961
                     /locus_tag="IVO69_001609"
     CDS             5635..5961
                     /locus_tag="IVO69_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7400389.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5977..6495
                     /locus_tag="IVO69_001610"
     CDS             5977..6495
                     /locus_tag="IVO69_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400390.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6495..7847
                     /gene="celB"
                     /locus_tag="IVO69_001611"
     CDS             6495..7847
                     /gene="celB"
                     /locus_tag="IVO69_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7400391.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7931..9436)
                     /locus_tag="IVO69_001612"
     CDS             complement(7931..9436)
                     /locus_tag="IVO69_001612"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7400392.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9528..10118)
                     /locus_tag="IVO69_001613"
     CDS             complement(9528..10118)
                     /locus_tag="IVO69_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7400393.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10297..11943
                     /locus_tag="IVO69_001614"
     CDS             10297..11943
                     /locus_tag="IVO69_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7400394.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11992..12711
                     /locus_tag="IVO69_001615"
     CDS             11992..12711
                     /locus_tag="IVO69_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7400395.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13190..14137
                     /locus_tag="IVO69_001616"
     CDS             13190..14137
                     /locus_tag="IVO69_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400396.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14149..15069
                     /locus_tag="IVO69_001617"
     CDS             14149..15069
                     /locus_tag="IVO69_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7400397.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15110..16567
                     /locus_tag="IVO69_001618"
     CDS             15110..16567
                     /locus_tag="IVO69_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7400398.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16912..17526
                     /locus_tag="IVO69_001619"
     CDS             16912..17526
                     /locus_tag="IVO69_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7400399.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17519..19243
                     /locus_tag="IVO69_001620"
     CDS             17519..19243
                     /locus_tag="IVO69_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7400400.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19240..20691
                     /locus_tag="IVO69_001621"
     CDS             19240..20691
                     /locus_tag="IVO69_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400401.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6748 a         3530 c         4181 g         6451 t
ORIGIN      
        1 atagatggct tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aacggggtat
       61 gaattggatg cgacaccagt aactttcaag atcgaaaaag aacaagaagt ggcgatcttt
      121 ttaacaaaag agaatagaaa agttgctgat agctcagata taagaggaaa caatccaaca
      181 agcagtggaa ataagcatct tccaaaaact ggagaaacca tcattagcca gtttatattg
      241 tcaatattgg gtgtgctact tgttttcatc agtataaaat ttagaaagaa acgaaatata
      301 tgagctaaaa taaaaaattg aataagcata gtttttaaaa tcaactagta cccatcaatt
      361 gaatggaaac taccgatttt agaaggggta tacaaacaaa tattagtatc ggagtagaga
      421 aggttgaacc caaccagtaa ataagtaaag acaattttgt gctgcgttga gaagtgaaaa
      481 ataaaccttt gtagtaatac tagttaataa aaaatataca aaaacactgc gaaaatcatt
      541 cttgagtttc gcagtgtttt ttgcctaatg actcaagttt attgggtaat actttgattt
      601 tctttctaca aataatcagc gatcagttgt aattttttct ctagttttaa tggctctttg
      661 tcaataagga gaaaaacaag tcgggtcttt tgtctcggaa agcttcaacc aactcgtgaa
      721 taaaggttac gcctccttga aaggcgtgga aaaaccaagt aatcgatcaa tcaccaattt
      781 cgcatttggt agaacatgtt tggtgagctg aaagtaggtg gcgttcatgt ctgtaactaa
      841 gaattctatt tctttaggat tagtgcagct aaggaaatag tttgttaatc gaggaatttt
      901 ttgtgtaggt aaattttctc gattaatttt tctgtctcgc catccgcgga aataaaactc
      961 agtttatcct caacggaaac gtgagaacga aattcacaac cctcaacact caagacagaa
     1021 ttcttttgga agactttggt aaatagcttc tcaactcttt caatgtgcga ataacggttg
     1081 tcaaagatac ctaacaactt ttcgaaataa aaaataacga cactttttca gtcagtaaag
     1141 aagtgatttt atgtctgaca tgatttgaaa tcgagtgcct tggacggata aaataacttt
     1201 gagcgttcca atggcttcga cagtttttac aggtatagcg ctgctttttt aggcgtataa
     1261 ccattggcat atgattatat tattcaaagt ggacaatggt ttccttcttc ttgtttttca
     1321 ccaccattaa tttcctattt ccatctacca cagcagaacc acaatttcta cagacacatg
     1381 gagcagggaa gagaactgca gcaacgatca atgttttttt cttctgtaag gtttcgtaag
     1441 agacctctgt aatcatcaaa tttttgtcta ttgatctcag aatttttttg atagaaccat
     1501 tcataaagca tatcgttctc tcagttgttt attttgtggt gatttaatca tacaagaaaa
     1561 cgatatgttt ttttataaaa acatgaatgg gaaaaatagt tgacaagaaa atgacaacgc
     1621 catatgatgt tccatagtat ttacaacctg tcatagatga ctgtaaaaaa taaaagtaaa
     1681 aagaaaggac gacaacaggt ggatttttat cagtctctac aactagatcc gtttatttta
     1741 aaacaaaaaa tacgagaagc agcttctaaa aaagaaaagt gcatgtatat ttgttcattg
     1801 tttttacgat cattattcat cgtattgttt gccatttgtt ttatcattat aattacaaca
     1861 ttatttgaat cgaagcataa accatacgct gtggttttat tctgtatgtt gatgagtatt
     1921 cgttttgttg actttggtta taagatttcc cattcgatca taggtttagc aatcgtcatg
     1981 ttctcactat tagtagcacc gtatgtccag ctgatcaaat ggtcagttat gggcatgttg
     2041 attcatttta ttttactaag cagcatatta atggcaaccg ctagtgatcc caaaatgggg
     2101 aatgcttctt tgtacggctt ttcatatctc tttattgttt attcgttacc taaagattcg
     2161 ttgaacaaga atttctttac acaaacaagt agcttattat ttttgttttt ttgttggttt
     2221 tcagttctcc tttacagaaa acatcgagaa aagaacaaag acaaaagctt gtttaaagag
     2281 atttttttga aaggcatgta ttcgcaggaa aaaatttgga tgctcatcta tgcatttggc
     2341 attagtcttt tgatcgttgc aggagagtat gtcccttttc agcgattgat gtgggcaggg
     2401 tttgcatttt cttctattgt atctagctat ggattgatgt cgattggttt caaagaacga
     2461 gcagtagacc gaatcattgg ctcattgatt ggatgtgttt tatttattgg tatcagccaa
     2521 tttattcctt ttaactgggt cggtatatta ggtggtctag ctttaggtgt ttgttctacc
     2581 tatagatata aaacagtttt taattctttt ggtgctttgg caatcactgc cagtttattc
     2641 ggggttccag gagcagtaac gatccgcatt tttgagaata tccttggggt atgtttagga
     2701 atcatgtata ttggggtgac tgaaatactt atccgaaaaa taagagaaaa acacggtttg
     2761 aatcattaga tgagatcgaa taaaaaataa ggaaaaagtg aagatacaca tggatgataa
     2821 attgctatca ttccatgtgt atctttttac ggtaaatcag tgaaacgttc gactttcatt
     2881 tttaatttat actttttacg gagttcgttt atttgagcca tcatttcgga tttgtgatgc
     2941 ttttcgattg cttcttcatc tttccaacgg tcgatcagta aaagagattc tggatcttct
     3001 agtggaaagt aatagtcata tttctcgttt ccttcttctg cacgaatcgc tttgacaatg
     3061 tttctttcga ccatttcttc tgcaaatttt ctggcttgtc catttgtact tgtatacaaa
     3121 atattcactg tgatactcac taaatcccct cctgaaaaaa ttctatcact agtatatcac
     3181 ggtgccagaa attattattc aagatatgat acacattttt tccgcaatgc tacggaaata
     3241 tatattttga atagaaaacg cttacaagat atgcttggtt tataaaaata aaaggagtga
     3301 ttgatttatg aaaaaagcat tgattatttg tgcggcagga atgtcatcct cgatgatggc
     3361 ctcaaagaca acggatttct ttaagcaaaa aagagaagag gtactcgtag atgcagtttc
     3421 agctacagaa ggcgataata tgattaagaa tagtgatttt gatttatttc tgatcagccc
     3481 gcaaacgacg atgtttttgg ataaattcaa aaaattagga gaatctgttg gaaagcctgt
     3541 tgttagtatc cctttccaag cgtatgtccc aatccctaca ggcatccaga agttagcaga
     3601 ggtgatcgaa gagaacatat gatggaatag gagtggatga taatgaaaca gaaggaaaaa
     3661 ttgctgttga gatatttgat tgatcacaag aaggagtatg tcaccagtca acgtttagct
     3721 tctgaattgt tattatctga tcggacaatc cgtaattact tgcagcggat caaagagtta
     3781 gtagaaaaaa acggaggtaa gatcattgct aaaccaggtt atggctacca actgcatatt
     3841 ttacaaagac tgacatttga cttgtttttg agtcggcaag aaatagtacc tggatatgct
     3901 agagaaccgc aaggatttta tgagtccgaa gatcgacaaa agtatatatt gaaccaattg
     3961 cttttggaag atgccattct ttttatggat gatttggcag aagagcttta tatcagtcgt
     4021 tccagtttga caaaggacat gcaagaaatc aaggagcgat tgattccata ttcactaaaa
     4081 atcgtttcta agcatggtca agggacatgg atcgaagggg aagaaagaaa tcgacggcat
     4141 tttatcatgg atactttttt tggaaaccat tatggaaatt cattgaaaga atatttaggg
     4201 caaagccgtt ttttcccaga catcagtttt gaagagctag tcattatcat tctcgatgaa
     4261 acaagagaag ctaagttaaa agtttcggat tttatcatac agaatctgac tttacacttg
     4321 gctttgggga tcaaacggct ccgagaaggg tttgaaatca aggaattagg catcgaacaa
     4381 gaagtttcta agcgagtaga gtatcaagtg gcgcagcgga tcgttcaacg tattgaggta
     4441 atcgcaggtg tatcgtttcc cgaagaagaa attgcttatt tgacattaca tctcatggct
     4501 aagtcgaatc acaaagaaaa tcaaaatgat caggaactat caaaggaatt agcgaaagtt
     4561 ctgaaaagat tgtctcagga atttcatttt tcattagtag aggatctcca gcttttcaat
     4621 ggactactcg atcatttgtc accgatgatg attcgattga gccgaggtat tcgcattgag
     4681 aacccattga ctgaggagat aaagaaagaa aatcccaaag tctttgacgc agtaaaaagg
     4741 catttcagca acatgccggc tttgaaaaac tataccatca atgaagatga atgggcctat
     4801 ttggctttgc atctcatggc ggcattggaa aaagaaagag ctgcgcataa actacatgca
     4861 ttgattatct gtgcaacggg ttacggcagt gcccaactat taaagaatcg cgtagtcagt
     4921 gaattcggta aaaatattac agtggtaagt gtaaaaggct attatgagat caatgaaaca
     4981 actttaaaag gaatcgacct gattatttct tctattgatc tttcgacaat gtttttcaaa
     5041 attccagttt tacatgtcag tatttttttg aatgaagatg atgttcgtaa aatacgaaag
     5101 gtagttggag aatctatacc ctatcattcc tcagatagac cgataccagc attttctcta
     5161 cagcaaaaaa agcagattta caccgaacag ttgtctgaaa aatttttcaa gacttatacg
     5221 tacgaaccga aaaaggaaga aattcttcat gatttgctgc aaaagctgag cataaatgaa
     5281 gaagaaggat atataagaga aatgcgaaag cagatccagc aacgtgaaaa tatgggacaa
     5341 atcatattca gtgatacagt ggcagtccca catcccgctg ttccagttgg aatcgaaaca
     5401 aaggtagccg ttgcattgat tccttctggg atgcagtggg atcaatatca agaaattcat
     5461 tttgtttttt tagtttcgcc ttcttatgtc gaaaatgaag gaattacggt cgtgactaaa
     5521 gcaatcgtta ggttagttga ccagctaaaa gtacagcagg aaatcttagc tgaacctatt
     5581 tttgagaatt tcagcagtaa atttatcaaa ctgatcgaat aaggaggaaa acagatggct
     5641 gaaaaaataa acagtgaaac actacaagtg acttcttttg atatcatttt ccatagcgga
     5701 aatgcacgga cactgataca tgaagcattt cgctcgatgc gtaaagagat gtttgaagaa
     5761 gtagaagaga agttgcgaca agcaaatgaa gagattcttc atgcgcatca gtcacaaact
     5821 caacttctga aagaatatgc cagcgggaca aaaatagaaa tggaaattat catggtccat
     5881 gcacaagatc atctgatgac gacgatgaca ttattggaag ttgcacagga aatgaattat
     5941 ttgtatcaaa aaaataaata gaaaggaagg atcattgtgc aggatacagt aaaaaggaaa
     6001 atcgaattag aaaaaaagca acttagtttg aaaaacatgt atttcaatcg ttatctaatc
     6061 gtacgttacc tgactgcctt tttctttttc atgaacatgc aatggatgat tctgcttcta
     6121 tcagctaaaa gtttaggaag tagtttaccg atgattctga ttttagctat tttgcctgca
     6181 gtaggagaac aagtaaagct ttatcgaaaa caccaaacga acgttccatg gacaaagtgg
     6241 tattttttgt tccagagtgt ctttaattta ttagtgattc cagtgttgtt tacctcagga
     6301 tttacattct tgtatccttt tatggcaaat aacaatggag gacagttatt tgttttcatt
     6361 cttgttgtaa gcggtatttt tgccagtgca tttatacagt atcgtttaaa gaaaatttca
     6421 ctcaaccaag accaacagta tatccgtatt aaacaatatg aaaaagcgtt atatttagga
     6481 aaggagaata actaatgttt gattttttgc agaaatacct gatgggacct atggggaaaa
     6541 ttgcgcaact taaaatcgtt cgggctgtca tggcagcggg gatggcttct attcctttca
     6601 cgattgtagg ctcgatgttt ttggttttaa atattttgcc attaccgttc ccatttttag
     6661 aaggattttt taatgcaact tttttcaaag ttagtgactt gtacatgatc gtcaatacaa
     6721 tgacgatggg gattttatct atttactttg ccattgtgtt tgcttatgaa ttaacgtcaa
     6781 ttgaacgaga tgaacaaaaa ttaaatgtta atccgctcac gggggcatta ctatctgttt
     6841 ttgcattctt tatgtgtatc cctgaattga tcatgactga tggtaaaatc agcttgattg
     6901 cttctatcaa tgagacagaa actgtagtaa atggtattag gatgggctct tttgtcgaac
     6961 gtttaggaac ttcaggaatc tttacagcta ttattatgtc gattattgcg gtcgaattgt
     7021 attgtatgtg tgtcaagaga aattgggtca taaaaatgcc agatgttgtt ccgccaggag
     7081 tttctcgttc tttcactgct ctgatcccaa catttgtcat tgcgtttgtg gtgatgatca
     7141 tcaatggatt attagttgca ctaggaacgg atatcttcaa aatgattgct atcccatttg
     7201 ggtttgttac gcagttgacg aatacatggc ttggtgtcat ggtgatttat ttcttgatcc
     7261 atgcattgtg gatcgtaggg atccatggag cgaatatcat cacttctttc ttgaccccga
     7321 tcgtattagc taatatggca gctaacgcac aaggcgcaaa ttatccgttg gcaggagaat
     7381 tcaacaattc ttatgtaacc gttggtggat caggtgcgac tttaggattg atcatcttta
     7441 ttgcattcct cgcgaaatct gatcagctga aagtgttagg aaaagcctca cttgtaccag
     7501 gattattcaa tatcaatgaa ccaatcattt tcgggatgcc aatcgtctac aatccctatt
     7561 tagcggttcc gtttttctta gctccgatgg cttctgctac actcgcttat tttgccattc
     7621 ggctggaaat cgtcaaaccg atgcttgcac agatgccgtg gccttcacca gtcgggatcg
     7681 gagcatttgt tggtagtggt ggtgattgga aagctgctgt gttagcagtc atttgtgcaa
     7741 tcatcgcatt cattatctgg ttcccattta tcaaattcta tgataataaa ttattaaatg
     7801 aagaaagaga aaaggcagca gaattagcta gtgaaaagtt aaaatgattt ctaagaaaaa
     7861 aggaactgtg acaaaaaatc acagtccctt tttttcttag aaatgggaaa ccactagtat
     7921 ataaatacca ttattcttca atcaaccgct tcacgattgc ataatcccct tcataaggtt
     7981 catctacacc attgattttt tgatagggat ccacgccttt tcccgcaata acgactgaat
     8041 cttgcggagc gctttctgct aaagcgcgtc gaatcgcttc aggacgatcc atttcataat
     8101 gaacgatcaa atctggatta gtaattgctt cattgatttc ttccgcgatt tttttcggat
     8161 cttcaaatgc tgggtcatct gccgtcaaaa aggcgatgtc agctgtttct gatagcactt
     8221 ttccaaaatc atgtcgacgg gagattgctt tgtttcctgg gctgcctaat acaacaatta
     8281 cacggccatc tggatgttca tttttggcaa atttcagcaa tgttttcaag cttagataat
     8341 tatgtgcata gtccacatag acatgcgctc cattggattg gatcaattga tccattcggc
     8401 ctggtacacg agcttctttt aatccgtctt gtgcatcttg actggaggca cctgttagtg
     8461 ctgcggcaat gatcgcactg gctgcatttt cttggttgaa tcctccagcc agcaaaatat
     8521 cataagcagt atcgcagata gctaagcgat cttcatgaga agataaagta aaaccgtgag
     8581 tacctttatc tgaacggatc acttgataat tgctttcttc cgaacgacca taagtgatcg
     8641 ttggaatctg gaatagatcg gctgtttccc taagtaaatc aaaatagtca ctttcatgat
     8701 ttaaaacaac tgtttttgaa ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa
     8761 atgtcggatg ttcgatcgga ctgatatggt caggcgagat attcaagaag attccgacat
     8821 ctaaagtcaa gccgtatacg cgttgtgttt tgtaagcttg tgaagaaact tccatcacta
     8881 gatgagtcat accattttca actgcttctg acatcatacg atataaatct aatgactcag
     8941 gcgttgtcaa atgggatttg aaatatgttt tgccatctaa cgttgtattc atagtagaaa
     9001 gcaatgctgt ttttttattt gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag
     9061 ttgttttacc ttttgttcca gtgaatccga tcactttcaa tttattttgc ggatagtcgt
     9121 aaaaagccat agcaagaagc gccattgctt tacggatatc cgttacgatg attcctactg
     9181 ctgaattttc gtatggttgt tctgatacat aatattgaat accctgttcg attgctgaat
     9241 ccaaatattc ttctttgaaa ttcattcctt tacagaaaaa caaagtagaa gcatcagctt
     9301 ttcttgaatc ataacttaac tgaagaaatt cagctttttc tggtacatca agatgccagc
     9361 ctcttgttga aacaaattct tttaatagat tttcttttaa tagtaaatga cggatgtctt
     9421 ctaaagagaa gctcattgat tgatccctcg ctttattcaa tttcttggtt tattatagca
     9481 cactcttttt aatatatcgt ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt
     9541 cttgacacag gggacaagcg gatgagtatt ttcataaaca atgaactgac tgtaatagat
     9601 aggagaacga atagtgtctc caacaatcca aaaacataat aattcaaaaa gacgataaga
     9661 ctattggtaa gtaaaagtcc ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc
     9721 ggcggtatcg ttataccgcc atttgaggcc ccgactgtgt acaatgtgta aaccccaaga
     9781 ctgaacaaaa gacttcctga aatcagtgaa aagaaatgtg aggagatcat ttccactttt
     9841 ggaacaatct ctaaaacaac aggaaaaagc agacttcccg ctacagtctt aaaaaatact
     9901 gattttccta ataggacagc ggctagaagc agcatcataa gattgatgct ccaaacagta
     9961 actgagcgtt ggatagaaaa ggccgtttca actaaatgtc cgatggcgcc cactccggcg
    10021 gaagctatat cgtgagggag caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct
    10081 aaaaatataa cgggtagttt ttttatttct tgtgtcattg atatctccta taaatgttag
    10141 ccataaaagg ctgtatattt ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac
    10201 aattgttctt ataaaagtta tataaaatcg attgccagtt tctgttttac caattaaaat
    10261 gatagtaatg ttttgtagaa agttggaatc tgaccgatgg ataatcaaag aaaaccagca
    10321 ttagatcaat taacaccgca agaaaaaatg gcacgaggct ctgcttggat gaccgccagt
    10381 aatatcattt caagattgct aggcgctgtc tatatcattc cctggtatgc ttggatgggc
    10441 gaaaatgcta aggctgccaa tggattattc aatatgggat acaatatata tgcattgttt
    10501 ctactgatct ctacagcagg aatacctgct gcgattgcaa aacaaactgc acgctataat
    10561 tcattgaatg aatatggcac ttcacgacga ttatttatcc gtgcattaca gatgatgggc
    10621 gggttaggcc tgttgtttgc tttgttcatg tacatcgcct ctccttggct agctcatgct
    10681 tctggtggcg gagaagaact gattccaacg atgcgttctt tgagtatcgc tgtattggtt
    10741 tttccttgta tgagtgtgat acgagggtat ttgcaaggca atcaagaaat gatgccttat
    10801 gcgctgtcgc agattgtaga acaggtggca cgtatttttt atatgctatt ggctacattc
    10861 atcatcatga aagtcatgga tggtgactat gtgacggcgg tcacgcaatc aactttcgcg
    10921 gcatttatcg gtatgctagc aagtattctt gtccttttgt attttttgaa gaaacatcaa
    10981 gcgtatacat cggcgtttat tcactacagc gaaaacaaaa taaatattgc aacgaaggaa
    11041 ttgttgttag ataccgttaa agaagcgatt ccgtttatta ttgtgggttc aggggtaacg
    11101 atcttcaaat tagtcgatca gttcactttt atgaagatca tgtctgcatc caccgagtat
    11161 tccaatgcac agttactgga tcttttttcg atttttagtg ccaatccaga taagttgacg
    11221 atggtagtta ttgcattggc aacatcgatc gcgtctactg ggttaccgtt gattacagaa
    11281 gctgttacgt tgaaagatcg agtaggtctg gcaaaattga caagcggaaa tttacagttg
    11341 ttttcatttt ttatgtttcc ggcgacattt ggtgttatgt tacttgccta tccgctaaat
    11401 acattatttt atacaccgga ttctctagga agtaatgtac tgatccaagc aagttttgta
    11461 ggactcttcc taggactata tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac
    11521 aaggcggcaa ttaattattt ggttattggg tttctagtga aactccttct tcaataccca
    11581 gccattcgtt tatttgaagt atacggccct ttattggcga caatgatcgg atttgctgtg
    11641 tcttgtacct tgatcttaaa gagaatccat gaagtagccc gctttaaacc aagttttgtc
    11701 tggcgcagga cattgctgat ctttattctt accttgatta tgcttttggc ttcgtggatc
    11761 acaaagatga tttttggtgc ttttttaaac gaagatagca aattccagtc tctattgttg
    11821 attcttgtag tagcaggtat aggggcttta gtgtatggtt atttagcttt gaaaatccgc
    11881 ttggcagaaa gactactagg accaggaata gcgcgtttac gacataaatt aaaaataaaa
    11941 taagtgaatc agaagagcta gattgatgct cttttgaaat gggggaaacg catgcgttta
    12001 gataagtttt tagcagatat gggacttggc agccgcaaag aagtcaaagg attaatcaaa
    12061 aaaggaacga tcgaagtcaa tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa
    12121 ttcgaagatg cggttactta tttaggagaa atcattacgt atcaaaaaga tttttactac
    12181 attttgcata aacctgcagg agtcatttct gctactcagg ataatcatga tcaaaccgta
    12241 atcgatttat tggaagacca ggattatcgg gaagatcttt ttccagtcgg ccgattagat
    12301 aaagacacag agggcctctt gattttaacg aatgacgggg tgtttgccca tcagttacta
    12361 tcacctaaaa aacatgtgga gaaagaatat ctagctgagg ttagcggtat aatgacagaa
    12421 gaagatatcc gtttatttgc agaaggactg attattgatg gcgatgagca gacattgccg
    12481 gcaaaactcc aaattgatca agtagatcaa gagacagaaa cttcaattat ccgtctgatt
    12541 ttgcatgaag gaaagtttca ccaagttaag cgaatggtca aagcagtagg aaaagaagtc
    12601 ttatatttaa aaagaatccg tatgggaaaa ttttggctgc ctaaagattt gaataaaggg
    12661 caatatcgac ccatgaccaa agaagagcta gaacaagtga gagaaaaata attgaatgga
    12721 tagctaaaag aataagcaga gtagttgtag cgccatctga aaagcagact tgtccgtctg
    12781 actttcggat ggcttttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac
    12841 accgtttgtt cggaataagg gactgtgaca agacttttgt tacagtcccc attgttcctt
    12901 gtgactcaga gttagatagt aaggacacga tctccctaat actcaggcca aaacgctgaa
    12961 ttcacaacct ctgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    13021 gcaaaatatg aggaactatt ttcccgtttt tttctctaat actcaggcca aaacgctgat
    13081 ttcacaacct ctcctatact ttgtttcatt ttccctatac tttgtttatt tattctcatt
    13141 attgaaagcg ctataattac gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg
    13201 aaaggggttc aatcattttt ttatgaatct atggcgttat aaagcacttg tcctgatggc
    13261 tgtaccgggt atgctttgga tgattttttt cttttacata cctgtaatgg cgaatgtcgt
    13321 agcattcaaa aattttcata tttcagcaga tgggttcttg gctagcttga gggaaagtcc
    13381 ttgggtgggt tgggaaaatt tcaaattttt attttcttcg gatcaagcat ttctcatcac
    13441 taaaaataca attttatata atgtagcatt tatccttttg aatctgctga tctcagtcgt
    13501 atttgcaatc attatgagcg agttgagaaa taaacgactc gttaaagttt atcaaacgat
    13561 gtccttacta ccttattttc tatcttgggt cattatcagt tactttgttt acgctttttt
    13621 aagtcctgac aaaggaatat tcaatcagtg gatcactgga catggaggag aagctgtcaa
    13681 ttggtataat gaaccgaaat attggccgtt tatcttggtg ttcattggta catggaaagg
    13741 gattggttat aacagcatta tttactttgc atctgttatg ggaatcgatc cgacttatta
    13801 tgaagctgct atggttgatg gagctagcaa atggcagcag atcaaacatg tgacgatacc
    13861 acaacttatt ccactaatga cgattttaac gattttagcc gtaggaaaca ttttccgagc
    13921 tgattttggc ctgttttata acattcctag gaattccggt gcattatatg atgtgactca
    13981 agttttggat acgtatatct acaatggcct gacatccaca ggagattttg gtatgacggc
    14041 tgctgcagga ctttaccaat cagttgtcgg ctttgtcttg ttgatgatca caaatacgat
    14101 cgcccgacgt tttgatagcg attctgcatt attctaagga ggaaaaaaat gaaaaaaaag
    14161 aaagtggaga gagtccaagt ccgctctttc aataagacgg ctaatttttt ctctaatata
    14221 atgattgcac tctttgctat ttcctgtatt ttgccattta tatttgttat cgccatctct
    14281 tttaccgatg agtccatatt gtcgactcac gggtacagtt tttggccaga aaagttcagt
    14341 atatttggtt atacgtactt gttcgaccag atgcaggata aaattttcca agcgcttttc
    14401 gtcactgtac ttgttactgt tttaggtaca ctgatcaata gcacggctac ttctttatat
    14461 gcttatgcga tttcacggtc aaactttcca ttccgtcgtt tttttaccgt tttttgtttg
    14521 attacgatgt tgttctcacc agggatggtg gccaattatc ttgttatgac aaatcttctt
    14581 cagttaaaag atacgatttg ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt
    14641 attgtgatgc gcacattctt taaacgttcg gtctctgatt cgattattga atcggcacgg
    14701 atcgatggag ctagcgaact gcgaatattt gttcagatcg ttttaccgtt agctgttcca
    14761 ggtattgcga caatcagttt atttgccgca ttgggttact ggaatgactg gttcaatgct
    14821 ttactataca tccaagatga taagctagtt ccactacagt atcttttgat gaaaatccaa
    14881 agtaatattc aatatctgac acaaaattca ggagcaggaa gccaattgac aggcggatta
    14941 gcctcagttc caggagaatc tgccagaatg gcgatcgtag tcatctcgac gttgcctatt
    15001 gcgatcagct atccattttt ccagaagcat tttgtcaaag gtctaacaat cggcggagta
    15061 aaagaataaa gaaacaaaaa tataaatcat aaaattagga ggaagaaaaa tgaagttatg
    15121 gaagaaacta gcctttacag gtgtttcagc gttgttgttg ggaacattag cagcatgcgg
    15181 aggttctggt tctaaagacc aggcagaagc tagcaattcg gacactttgc agatgtatca
    15241 aatcggagat aaaccagata atttcgatca attgatggaa gtagcaaaca aacgaatcga
    15301 aaaagagatc ggtgtaaaag tcaatatcaa ttacattggc tggggcgatt acgaaaagaa
    15361 aatgaacgtc atcatttctt ccggggaaaa ttatgatatc gcctttgcga acaactatgt
    15421 ttccaatgcc caaaaaggag cctttgctga tttgactgaa ttagcgccta agtacgctga
    15481 aaaagcttat aatgatttag atgaagcata tattaaagga aatctggtca atggaaaatt
    15541 atatgctttc ccagtcaatg gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc
    15601 tttactagat aaatacgatc tttcaattga tggaatccaa tcttacgctg atgcagaaaa
    15661 agtcttgaag gaattccatg aaaaagaacc aaacacggca gcttttgcca ttggacaagg
    15721 cttcaaagtc caagggaact ttgattatcc tttagggaat acgttaccat ttgctattga
    15781 tctggatgga gatgattcga aaatcatcaa ccaatacgat aatgaaacgt atatcggact
    15841 gttacgtaca atgcatagct ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag
    15901 caataaggat tatccactag aaggaaatac ttggctaatg cgtggagaaa cacaaggacc
    15961 gtatgattat ggcgatacaa ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc
    16021 aattactgtt ccactaaaat ctacagctca agcacaaatg gcaaattttg tcgtatcaaa
    16081 tacttcaaaa aataaagaaa aatcagtaga acttctaggg ctgcttaaca gtgatccaga
    16141 attattgaat gggcttgttt gggggatcga gggagaagca tgggaaaaag tcgatggttc
    16201 agatgataaa atcaaattgc tagatggtta tcagccaaat acacatatgt ctgcttggaa
    16261 tacaggaaac aacaagattc tatatacaca agagtctatt acggatgaca tgattgccaa
    16321 acgtgatcaa tcgattgctg atgcagaaac ttcaccgatt ttaggattca gcttcaacac
    16381 tgacagtgtc aaaacagaat taagtaatat ttctaatgtc atgaatcaat atttagatgg
    16441 attgaataca gggactgttg atccagatga aacattaccg aaattgaaag acgctttgga
    16501 caaggctggc tatgataagg tattgaaaga aatgcaaaaa caatatgatg agttccgaca
    16561 agaataataa tgaataaaga caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt
    16621 ccgaataaac ggtgttcaaa agccagtttc tcggtattaa gctaaatata tgtaaaaatg
    16681 tggaaagcca tattcacaaa atttttctta ctactcgaaa ctaagcggct atttcacaaa
    16741 ctcttggtaa acggtgttca atcagctaac cctcggtatt cgctcacaaa acgacaaaat
    16801 atgaggaact atttttccgt ttgtttctct aataatcagg tcaaggtgct gattgcccaa
    16861 cctttttttg tataatgaga aagtataaac ttaatgtttg gaggagaaga tatgttaggg
    16921 aaggttttgg aagagttatt tatccggatc tgggtcatta taaaactaac cttgtatttc
    16981 tggatatata catttgccgg cgggatcatc tttggattag gtgcggcatg gaaaacagtc
    17041 aatgaactat tttatttgta cggattcgag tataaagaaa tcacaataaa aagaggctgg
    17101 aatatatata aaagaaattt tttaagaggc aatctgctat ttagtttatt tttatctggt
    17161 actgctttat tgagttacaa tttatattta tctgttcaaa tcaaagggct cattttttta
    17221 gctattgatt ttattttact ttttgccttg ctctgcctat ttgctactta tcaatacagt
    17281 atgattttag atagtgaata tacgatctct gttcctaatc tgctaaagct aagttttatt
    17341 tcggtttttt ccagcttttc gtcttttcta aaaataatta ttggtagcgg tataatttta
    17401 ggcgtgactt ggcaattcaa aggattgatc ctgtttggtg tcattggtct tttgacggtt
    17461 tggaatggta cgatgacaac acattggcgt gaagaattag ataaacagtt ggaaagttat
    17521 gaataagttt cggattctat tttcaaaagg aaccttacta aatcgtctgt tgcggaaata
    17581 cgcggttatc atgatcggtg taacgatgac tgctaccgtt atttttagta tccatacttg
    17641 ggaacaaaat caaaaacagg cagaaaacat gacaagtgat gccgttcaaa gcacaagcag
    17701 aatgctgaat gataaaacaa ccttgagtag gatcatcaaa aaccagcttg tagggaattc
    17761 cgaaaagatc gaaaatgtga cgacctactt aacaaaaccc atagatcagt atctgatgta
    17821 tgtatacgaa cagcaaaaca gtaccgatga attagtttct ttcccaaatc aaatcaaaga
    17881 tctctacgca aattatgaag aactaagtgc gatatatatc gtgttgaatc aactaccgga
    17941 gtattatgaa tcttctagag aaaacaaagg tggggaaata aaaaagggga caccaaaatt
    18001 actagacgcc ttttatatca agttgcctat cacacaagga ggagctgagt caggaagtat
    18061 ttttattggt tttcaaaaga aagaactaga tatgatttta gaaaatctaa cgtcattcaa
    18121 tggactctct ttatacatga tttctggaac gacaaatcga ctatacacat tccatgataa
    18181 taaaatagct aaagaaacgt ttgaaagaca agaaatgatc atcacagaga gtctgaagaa
    18241 aaattcacaa ttgccgattg acgatctgga aaaaaacaat tttgttcaac accaagaact
    18301 gtctgatgat tatcgtatat tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg
    18361 aaaaaatctt gccccgctga tcattggtat cattatttta gacaatatac tgcttatctt
    18421 tctttataaa acattcaaac gatattcaca gcaagtatct atcgtcatgc aggccatgga
    18481 tcaaacagca gcagggaatt tagagacgcg gatcgacata acaaacacgg aatatgaact
    18541 gaaggaactt tcaatcggta tcaacgaaat gttggatagc atcaatcagt ttgtagaaga
    18601 catctacaaa ttagaaatca aacagcaaga cgcacatatg cgggcgcttc aagcacagat
    18661 caatccacac tttttatata atacattaga atatatccga atgtatgcaa tcagcgaagg
    18721 aagtgaagaa ttagcagatg ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa
    18781 tcaagagaaa acgatcactt taaaagaaga actggacttt tgcgaaaaat atgtctattt
    18841 gtatcaaatg cgttatccta atcgagtagc ctatcatttt atgatcgatc ctgatttgga
    18901 aaagatcgaa gtacctaaat ttgtgatcca gccattagtt gaaaattatt ttaaacacgg
    18961 tattgatttt actcgattcg ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg
    19021 ggtaaggatc atcatcaaag ataatggaaa agggatgact gaaaaacggc taaaacagat
    19081 tgaagaaaaa ctgtctcatc ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt
    19141 gaatgaacgt ttacgtgcca gcttcggcag cagttattat atgtcgcttg agaataatga
    19201 gacaggtgga ctgaccgttt ctattacatt caaggaggga tgagcgtgta tcaagtgtta
    19261 ttggtagatg atgaatacat gattttgaat gggttaaaaa agataattga ttggcaaagt
    19321 ttaggctttc aaatagtagc aacagcagag aatgccaaag aaggactagc tgtacttgaa
    19381 cagcgacaga ttgatttagt agtaacggat gtgacaatgc cagaaataaa tggactggaa
    19441 tttattgaag cggctcaaaa agaacgtcat aattttgaat ttatgatttt atcaggatac
    19501 caagaattcg attatttgaa aggaggtatg caattaggcg ccgtgaatta tttgatgaaa
    19561 cccgtgaata agttcgaatt ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag
    19621 caaaatgaac aaaaaaatca acaagaaatc tatcaagaac tgttattcag tcaatggttg
    19681 aatgaagaat tggatgaaat aagtgaagaa gaaataatag gacaaataag tgagaagcag
    19741 cataggatag tattgattca actggcaaga actcatgata ctcttatcaa tagatggttg
    19801 caagaacggc aacaaatctt ttactatcaa cgaaattacg gagaccgtgt attgatcacg
    19861 ttgttgttgg aggcagatga agttgatggc ttctgttgtt ttgttcaaga aaactttcct
    19921 gatcaggaat ggctgatcag tatcggggaa gagacagatt ctttggacaa aatccctgaa
    19981 acattccaac aagcgaaaga caatctacag ttgcatcaat tttatggaga taaagacgag
    20041 catgtttttt acgcagtaca agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc
    20101 agacgcgtgc tgcaaaataa acaattggaa gtcgctcaaa aaatgatcac tgattttttt
    20161 gaacagtttc agttagcagc catgatgccg gaagatatac gatattccag ttttttactg
    20221 tttatggaga ttcaacggga attgattgat ctggaagatg aggaatattt acaaggaatc
    20281 gaaaaaatcc atcaggcaaa aacggtgcaa gaacttcagc agctacttct gtcattcatc
    20341 aaaaagcatc agagacagaa aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg
    20401 caccagcatt atcaagaacc acttacactc aaagaagtgt cagaaaacct gcatttaaat
    20461 gtcatgtatc taggacagct gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg
    20521 aaccatttac gtatggaaaa agcgaaacaa ctgcttttgc acagtaatca aaacatcaat
    20581 gagatagcaa gtgagattgg ctacaacaac accacgtatt tctctaaatt attcaagaaa
    20641 atcgtcggca gatcccctaa agagtaccga gaaaacagtg gagaatcata aaagaaaaag
    20701 ccaatgtaat gaaataactt ataaaaaatt gtctagaaat ctaattttgg gctgttctta
    20761 ttacttgagg caaggcagct ttttcatgac ctctcgataa acggagttca aaagccagtt
    20821 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
    20881 taatactcga aactaaacag ctttttcacg
//
LOCUS       DACCPY010000051        19927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCPY010000051 DACCPY010000000
VERSION     DACCPY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.052"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..331
                     /locus_tag="IVO69_001622"
     CDS             <1..331
                     /locus_tag="IVO69_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7400402.1"
                     /translation="VLFIIPPVNEKWSDYTGLSQEMLQGFAKKIKFQLNSQGFNRIAD
                     FVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEENHITASKYHLDDAFFSKSWQHQI
                     PDKLQLK"
     gene            472..1554
                     /locus_tag="IVO69_001623"
     CDS             472..1554
                     /locus_tag="IVO69_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7400403.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1554..2357
                     /locus_tag="IVO69_001624"
     CDS             1554..2357
                     /locus_tag="IVO69_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7400404.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2427..3167
                     /locus_tag="IVO69_001625"
     CDS             2427..3167
                     /locus_tag="IVO69_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7400405.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3178..4182
                     /locus_tag="IVO69_001626"
     CDS             3178..4182
                     /locus_tag="IVO69_001626"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7400406.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4227..6071)
                     /locus_tag="IVO69_001627"
     CDS             complement(4227..6071)
                     /locus_tag="IVO69_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400407.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            6283..6801
                     /locus_tag="IVO69_001628"
     CDS             6283..6801
                     /locus_tag="IVO69_001628"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7400408.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6929..7612
                     /locus_tag="IVO69_001629"
     CDS             6929..7612
                     /locus_tag="IVO69_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7400409.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7921..7994
                     /locus_tag="IVO69_001630"
     tRNA            7921..7994
                     /locus_tag="IVO69_001630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7955..7957,aa:Arg,seq:tct)
     gene            8153..8512
                     /locus_tag="IVO69_001631"
     CDS             8153..8512
                     /locus_tag="IVO69_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7400410.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8635..10266
                     /locus_tag="IVO69_001632"
     CDS             8635..10266
                     /locus_tag="IVO69_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7400411.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(10328..10969)
                     /locus_tag="IVO69_001633"
     CDS             complement(10328..10969)
                     /locus_tag="IVO69_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7400412.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            11251..11559
                     /gene="rplU"
                     /locus_tag="IVO69_001634"
     CDS             11251..11559
                     /gene="rplU"
                     /locus_tag="IVO69_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7400413.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11586..11930
                     /locus_tag="IVO69_001635"
     CDS             11586..11930
                     /locus_tag="IVO69_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7400414.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            11943..12236
                     /gene="rpmA"
                     /locus_tag="IVO69_001636"
     CDS             11943..12236
                     /gene="rpmA"
                     /locus_tag="IVO69_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7400415.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12643..15477
                     /locus_tag="IVO69_001637"
     CDS             12643..15477
                     /locus_tag="IVO69_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400416.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            15668..16159
                     /locus_tag="IVO69_001638"
     CDS             15668..16159
                     /locus_tag="IVO69_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7400417.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            16262..17251
                     /gene="manX"
                     /locus_tag="IVO69_001639"
     CDS             16262..17251
                     /gene="manX"
                     /locus_tag="IVO69_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7400418.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            17284..18087
                     /locus_tag="IVO69_001640"
     CDS             17284..18087
                     /locus_tag="IVO69_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7400419.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            18108..19022
                     /locus_tag="IVO69_001641"
     CDS             18108..19022
                     /locus_tag="IVO69_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7400420.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            19105..19479
                     /locus_tag="IVO69_001642"
     CDS             19105..19479
                     /locus_tag="IVO69_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7400421.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            19618..19863
                     /locus_tag="IVO69_001643"
     CDS             19618..19863
                     /locus_tag="IVO69_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7400422.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         6411 a         3292 c         4217 g         6007 t
ORIGIN      
        1 agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg gactatctca
       61 agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa
      121 tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca
      181 tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa
      241 tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca
      301 tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt
      361 ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa
      421 tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca
      481 aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa
      541 gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac
      601 ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct
      661 ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa
      721 ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa
      781 acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca
      841 cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca
      901 agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt
      961 gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc
     1021 gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt
     1081 ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca
     1141 caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca
     1201 ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt
     1261 gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa
     1321 atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg
     1381 catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat
     1441 tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa
     1501 ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata
     1561 atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg
     1621 gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt
     1681 ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag
     1741 tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg
     1801 tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg
     1861 gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa
     1921 tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt
     1981 caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat
     2041 cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc
     2101 aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat
     2161 atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta
     2221 tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt
     2281 atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag
     2341 acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag
     2401 gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt
     2461 atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt
     2521 tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc
     2581 tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac
     2641 agtattactg gcaacttcat cctaagagaa atccggagac attgctttca atttatggac
     2701 acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc
     2761 cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa
     2821 tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg
     2881 tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt
     2941 tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt
     3001 caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc
     3061 tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag
     3121 ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg
     3181 atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg
     3241 ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc
     3301 atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat
     3361 ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac
     3421 ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta
     3481 acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc
     3541 cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac
     3601 ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat
     3661 gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt
     3721 gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt
     3781 cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac
     3841 gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat
     3901 tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct
     3961 atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc
     4021 tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag
     4081 aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt
     4141 ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa
     4201 tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga
     4261 acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc
     4321 gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg
     4381 cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt
     4441 tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga
     4501 gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg
     4561 ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct
     4621 aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc
     4681 atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg
     4741 tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta
     4801 tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg
     4861 atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga
     4921 ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt
     4981 gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag
     5041 acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat
     5101 gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca
     5161 ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg
     5221 aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag
     5281 atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt
     5341 tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca
     5401 aagcaactta agagaataag tgagtattca agacgttttt gtttatacat caagtagatc
     5461 gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc
     5521 actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt
     5581 ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata
     5641 taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa
     5701 tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct
     5761 ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt
     5821 tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga
     5881 tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca
     5941 ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata
     6001 atgattatgc tccactttat acgtaaataa aaagattctt ttttattata tatgttatta
     6061 aaaaaagaca taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa
     6121 actaacgtca attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac
     6181 atataattat taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt
     6241 atatgattaa gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa
     6301 ataactttta taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc
     6361 ttggttcccc gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca
     6421 tattatgcac aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc
     6481 ggagatatta ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata
     6541 gccgttgaaa acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag
     6601 cctggagtag taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt
     6661 ggactagtcc tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct
     6721 ttttgcctaa caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga
     6781 cgaacacaga aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga
     6841 ctcttctgaa catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt
     6901 cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt
     6961 caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg
     7021 caaactggaa gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt
     7081 atttttgaaa tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc
     7141 tatcggtttt tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac
     7201 aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact
     7261 gacagataaa gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg
     7321 tccgtatgac tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga
     7381 atgcttacgt ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg
     7441 aacgatcttg ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt
     7501 aaatgaattt cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt
     7561 aggtgatggt gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat
     7621 tatggcagcc tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt
     7681 ttctatattt gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga
     7741 agtcaatgta gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg
     7801 ttttaaaaga ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac
     7861 tatttcgtat tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt
     7921 gtcatagtag ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa
     7981 tccctcctgt gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt
     8041 ttttgtgcca atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag
     8101 tacaattata aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat
     8161 actatctaga attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga
     8221 aacggtgaga actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt
     8281 aagcagaaaa tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg
     8341 agtgggacta ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct
     8401 attaatatct attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt
     8461 actcaaacaa ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa
     8521 aaaataagaa tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata
     8581 tgatattcta atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc
     8641 aaaaattatg tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag
     8701 aaagatcttt tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt
     8761 ggggtatcaa gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc
     8821 gttattggcg gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca
     8881 gctatcttga tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt
     8941 ttagcgggtc tatttttact tgcagcagga cttttccgat taggaacact gacttctttt
     9001 atcccagcac ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt
     9061 cagatcgata atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt
     9121 agttatcaaa cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc
     9181 attttaggga tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg
     9241 gctatcattt tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt
     9301 aagattccac aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct
     9361 gccttgcaac aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc
     9421 ttgctatgtg gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa
     9481 ttaatggcgc aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact
     9541 gcggccatcg cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt
     9601 atgatacatg cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag
     9661 attccaatgc cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg
     9721 gaaacgatca aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt
     9781 acgatgattt gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc
     9841 ggctgtgtat tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat
     9901 tggcaaagaa tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt
     9961 agtggtccac tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat
    10021 catcacgatg gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt
    10081 gcagtttttg atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact
    10141 tccttgcagc cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat
    10201 caacatcaaa cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc
    10261 cattaaaaca atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct
    10321 gttcttttta atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag
    10381 gtacaaggga gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt
    10441 ttttttctga ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat
    10501 agagttgacc atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa
    10561 tgtttccaag tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg
    10621 gtgcatgcgc aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact
    10681 gttgtgtccg tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg
    10741 tgatatacac ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc
    10801 cagcttgacc gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca
    10861 cgagcatcag tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc
    10921 cgttcattct taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc
    10981 ctttttcttt tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact
    11041 gtgaggatag aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt
    11101 tagttgacta acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag
    11161 ctacaaccgc tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc
    11221 taagtatatc agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa
    11281 caagtaaaag ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga
    11341 gaaaaagttg tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct
    11401 ccaactgttg caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa
    11461 gtcgttactt tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa
    11521 ccatatacaa aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc
    11581 tatatatgat caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt
    11641 tgaccgggca tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg
    11701 cgttagcgat cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg
    11761 aaatgaatga agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag
    11821 aacaaaacaa tattgctgag attctattag aaaatctttt attaggatta caatcgatag
    11881 aagctgaaaa ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga
    11941 ccatgttgtt atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat
    12001 ctaacggccg tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag
    12061 ttacaggtgg atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg
    12121 tgggtatcgg tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac
    12181 gtaaaggccg taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga
    12241 atcttatgat taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa
    12301 taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg
    12361 ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt
    12421 ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa
    12481 aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc
    12541 aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt
    12601 tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac
    12661 cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa
    12721 ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa
    12781 gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt
    12841 tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac
    12901 aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt
    12961 gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg
    13021 attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag
    13081 acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa
    13141 acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt
    13201 catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt
    13261 atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa
    13321 ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca
    13381 acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct
    13441 tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa
    13501 aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac
    13561 cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg
    13621 acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt
    13681 ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt
    13741 cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata
    13801 ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc
    13861 tatgagttac cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg
    13921 gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg
    13981 aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt
    14041 gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta
    14101 ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg
    14161 aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt
    14221 gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt
    14281 caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg
    14341 gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca
    14401 agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac
    14461 atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa
    14521 gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat
    14581 tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct
    14641 ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat
    14701 gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa
    14761 gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca
    14821 gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta
    14881 gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa
    14941 tatcagatca tagctactac tgggattact gatttgaata tccaagcccc atttattcct
    15001 ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca
    15061 gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt
    15121 gagtttatct ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg
    15181 caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa
    15241 atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg
    15301 actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca
    15361 gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat
    15421 tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata
    15481 cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa
    15541 attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag
    15601 gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt
    15661 tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc
    15721 aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc
    15781 tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa
    15841 tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa
    15901 agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca
    15961 gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa
    16021 ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt
    16081 ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc
    16141 gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa
    16201 tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa
    16261 tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg
    16321 ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg
    16381 tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga
    16441 agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt
    16501 cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga
    16561 agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga
    16621 aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa
    16681 agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga
    16741 cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc
    16801 gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc
    16861 aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa
    16921 tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa
    16981 agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga
    17041 catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa
    17101 agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt
    17161 gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa
    17221 aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt
    17281 atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa
    17341 ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta
    17401 gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta
    17461 ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca
    17521 gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt
    17581 gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt
    17641 ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg
    17701 tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg
    17761 ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca
    17821 gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac
    17881 atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt
    17941 tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg
    18001 aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct
    18061 ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa
    18121 aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag
    18181 gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa
    18241 tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat
    18301 tctttaatac acacccatac attgcttctc caattttagg ggttacattg gctctggaag
    18361 aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta
    18421 tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc
    18481 taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct
    18541 tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca
    18601 aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag
    18661 gtgcttcaat tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca
    18721 aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata
    18781 aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag
    18841 ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt
    18901 tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa
    18961 tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat
    19021 aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca
    19081 taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta
    19141 attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat
    19201 ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa
    19261 gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt
    19321 caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt
    19381 gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta
    19441 gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag
    19501 aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta
    19561 tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg
    19621 agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc
    19681 agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt
    19741 attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca
    19801 tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca
    19861 taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg
    19921 ctttatt
//
LOCUS       DACCPY010000052        19886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCPY010000052 DACCPY010000000
VERSION     DACCPY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.053"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IVO69_001644"
     CDS             2..400
                     /locus_tag="IVO69_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7400423.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IVO69_001645"
     CDS             384..1079
                     /locus_tag="IVO69_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7400424.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IVO69_001646"
     CDS             complement(1673..2560)
                     /locus_tag="IVO69_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7400425.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IVO69_001647"
     CDS             2745..3584
                     /locus_tag="IVO69_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7400426.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="IVO69_001648"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="IVO69_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7400427.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="IVO69_001649"
     CDS             5052..5408
                     /locus_tag="IVO69_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400428.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="IVO69_001650"
     CDS             5411..6568
                     /locus_tag="IVO69_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400429.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="IVO69_001651"
     CDS             complement(6594..7034)
                     /locus_tag="IVO69_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7400430.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="IVO69_001652"
     CDS             7194..7700
                     /locus_tag="IVO69_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7400431.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9781
                     /locus_tag="IVO69_001653"
     CDS             7868..9781
                     /locus_tag="IVO69_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7400432.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9786..10787
                     /locus_tag="IVO69_001654"
     CDS             9786..10787
                     /locus_tag="IVO69_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7400433.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10989..11195)
                     /locus_tag="IVO69_001655"
     CDS             complement(10989..11195)
                     /locus_tag="IVO69_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7400434.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11841..12689)
                     /locus_tag="IVO69_001656"
     CDS             complement(11841..12689)
                     /locus_tag="IVO69_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7400435.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12853..13755
                     /gene="gnd"
                     /locus_tag="IVO69_001657"
     CDS             12853..13755
                     /gene="gnd"
                     /locus_tag="IVO69_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7400436.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13757..14758
                     /locus_tag="IVO69_001658"
     CDS             13757..14758
                     /locus_tag="IVO69_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7400437.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14755..15039
                     /locus_tag="IVO69_001659"
     CDS             14755..15039
                     /locus_tag="IVO69_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7400438.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15072..15869
                     /locus_tag="IVO69_001660"
     CDS             15072..15869
                     /locus_tag="IVO69_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7400439.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15883..16716
                     /locus_tag="IVO69_001661"
     CDS             15883..16716
                     /locus_tag="IVO69_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400440.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16734..17591
                     /locus_tag="IVO69_001662"
     CDS             16734..17591
                     /locus_tag="IVO69_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7400441.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17613..18122
                     /locus_tag="IVO69_001663"
     CDS             17613..18122
                     /locus_tag="IVO69_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400442.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18159..18560
                     /locus_tag="IVO69_001664"
     CDS             18159..18560
                     /locus_tag="IVO69_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400443.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18672..19025
                     /locus_tag="IVO69_001665"
     CDS             18672..19025
                     /locus_tag="IVO69_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400444.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19059..19502
                     /locus_tag="IVO69_001666"
     CDS             19059..19502
                     /locus_tag="IVO69_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7400445.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19519..>19886
                     /locus_tag="IVO69_001667"
     CDS             19519..>19886
                     /locus_tag="IVO69_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7400446.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENN"
BASE COUNT         6835 a         3054 c         4126 g         5871 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc
     9721 aggtggattt ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta
     9781 aatgaatgaa atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt
     9841 tagtcactgt atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa
     9901 aacaatttga cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa
     9961 aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata
    10021 atatggatat ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc
    10081 atggaggcgc atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat
    10141 gttcagacac gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac
    10201 atcctacacc agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag
    10261 atcaagcaga ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg
    10321 gagaatattt gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga
    10381 ctttggagaa caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg
    10441 cacttactca agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt
    10501 actttgataa taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac
    10561 atgcggatcg ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga
    10621 aacaaatgga agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag
    10681 attatgcgga gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac
    10741 aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc
    10801 aaaagaggtt gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag
    10861 cttttcatgg tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca
    10921 ccttttattc gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat
    10981 tttataagtc agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg
    11041 gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt
    11101 ccgttgttat tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta
    11161 gcgaaaaaaa gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt
    11221 gtatgataaa tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat
    11281 gatcatctag cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat
    11341 cagataggta caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat
    11401 aatagaaaag atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa
    11461 cttttttatt ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta
    11521 aaatgaggcc aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa
    11581 ttttttagaa aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact
    11641 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
    11701 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
    11761 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
    11821 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
    11881 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
    11941 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
    12001 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
    12061 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
    12121 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
    12181 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
    12241 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
    12301 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
    12361 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
    12421 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
    12481 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
    12541 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
    12601 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
    12661 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
    12721 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
    12781 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
    12841 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
    12901 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
    12961 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
    13021 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
    13081 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
    13141 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
    13201 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
    13261 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
    13321 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
    13381 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
    13441 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
    13501 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
    13561 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
    13621 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
    13681 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
    13741 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
    13801 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
    13861 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
    13921 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
    13981 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
    14041 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
    14101 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
    14161 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
    14221 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
    14281 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
    14341 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
    14401 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
    14461 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
    14521 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
    14581 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
    14641 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
    14701 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
    14761 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
    14821 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
    14881 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
    14941 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
    15001 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
    15061 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
    15121 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
    15181 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
    15241 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
    15301 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
    15361 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
    15421 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
    15481 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag
    15541 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
    15601 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
    15661 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
    15721 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
    15781 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
    15841 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
    15901 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
    15961 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
    16021 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
    16081 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
    16141 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
    16201 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
    16261 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
    16321 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
    16381 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
    16441 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
    16501 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
    16561 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
    16621 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
    16681 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
    16741 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
    16801 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
    16861 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
    16921 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
    16981 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
    17041 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
    17101 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
    17161 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
    17221 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
    17281 gtaatgctac aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag
    17341 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
    17401 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
    17461 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
    17521 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
    17581 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
    17641 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
    17701 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
    17761 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
    17821 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
    17881 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
    17941 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
    18001 atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag
    18061 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
    18121 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
    18181 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
    18241 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
    18301 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
    18361 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
    18421 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
    18481 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatccc catcacgtat
    18541 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
    18601 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
    18661 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
    18721 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
    18781 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
    18841 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
    18901 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
    18961 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
    19021 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
    19081 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
    19141 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
    19201 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
    19261 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
    19321 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
    19381 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
    19441 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
    19501 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
    19561 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
    19621 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
    19681 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
    19741 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
    19801 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
    19861 actgatttgt ttaatgaaaa caacga
//
LOCUS       DACCPY010000053        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCPY010000053 DACCPY010000000
VERSION     DACCPY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.054"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IVO69_001668"
     CDS             133..3660
                     /locus_tag="IVO69_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7400447.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVO69_001669"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVO69_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7400448.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVO69_001670"
     CDS             7448..7789
                     /locus_tag="IVO69_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400449.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVO69_001671"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVO69_001671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7400450.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVO69_001672"
     CDS             9146..11566
                     /locus_tag="IVO69_001672"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7400451.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO69_001673"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO69_001673"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7400452.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELDELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVO69_001674"
     CDS             13000..13737
                     /locus_tag="IVO69_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7400453.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVO69_001675"
     CDS             13751..14566
                     /locus_tag="IVO69_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7400454.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVO69_001676"
     CDS             14579..15226
                     /locus_tag="IVO69_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7400455.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVO69_001677"
     CDS             15242..15898
                     /locus_tag="IVO69_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7400456.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVO69_001678"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVO69_001678"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7400457.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVO69_001679"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVO69_001679"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7400458.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVO69_001680"
     CDS             18240..18758
                     /locus_tag="IVO69_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7400459.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVO69_001681"
     CDS             18865..19365
                     /locus_tag="IVO69_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7400460.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4176 g         5398 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcatctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACCPY010000054        19225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCPY010000054 DACCPY010000000
VERSION     DACCPY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.055"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IVO69_001682"
     CDS             168..362
                     /locus_tag="IVO69_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400461.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVO69_001683"
     CDS             631..930
                     /locus_tag="IVO69_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400462.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVO69_001684"
     CDS             complement(1027..1461)
                     /locus_tag="IVO69_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400463.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVO69_001685"
     CDS             complement(1693..2403)
                     /locus_tag="IVO69_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7400464.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVO69_001686"
     CDS             complement(2393..3253)
                     /locus_tag="IVO69_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7400465.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVO69_001687"
     CDS             complement(3258..3896)
                     /locus_tag="IVO69_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7400466.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVO69_001688"
     CDS             complement(3911..4210)
                     /locus_tag="IVO69_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400467.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVO69_001689"
     CDS             complement(4253..5737)
                     /locus_tag="IVO69_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7400468.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVO69_001690"
     CDS             complement(5757..6218)
                     /locus_tag="IVO69_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400469.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO69_001691"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO69_001691"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7400470.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVO69_001692"
     CDS             7521..8291
                     /locus_tag="IVO69_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7400471.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVO69_001693"
     CDS             complement(8564..9391)
                     /locus_tag="IVO69_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400472.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVO69_001694"
     CDS             9959..11194
                     /locus_tag="IVO69_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7400473.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVO69_001695"
     CDS             complement(12193..12453)
                     /locus_tag="IVO69_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7400474.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVO69_001696"
     CDS             complement(12480..13781)
                     /locus_tag="IVO69_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400475.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVO69_001697"
     CDS             complement(14073..14531)
                     /locus_tag="IVO69_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7400476.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVO69_001698"
     CDS             complement(14544..15869)
                     /locus_tag="IVO69_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7400477.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVO69_001699"
     CDS             complement(15886..17298)
                     /locus_tag="IVO69_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400478.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVO69_001700"
     CDS             complement(17315..18079)
                     /locus_tag="IVO69_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7400479.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVO69_001701"
     CDS             complement(18183..19058)
                     /locus_tag="IVO69_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400480.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6116 a         3952 c         2947 g         6210 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taata
//
LOCUS       DACCPY010000055        18913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCPY010000055 DACCPY010000000
VERSION     DACCPY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.056"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(61..381)
                     /locus_tag="IVO69_001702"
                     /pseudo
     CDS             complement(61..381)
                     /locus_tag="IVO69_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            525..1964
                     /locus_tag="IVO69_001703"
                     /pseudo
     CDS             525..1964
                     /locus_tag="IVO69_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(1971..4523)
                     /locus_tag="IVO69_001704"
     CDS             complement(1971..4523)
                     /locus_tag="IVO69_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7400481.1"
                     /translation="MNWYKLTVEDTLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIVKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHTVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFSYGMDWPILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVNEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLSNMKNCQELKTKEEQKTENLSGNPTELALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHTEGKGAVIYVKGAPEVLLAKSTLSSSEQEAWGQTATAFAKK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILILALILNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPS
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR"
     gene            complement(4636..5967)
                     /locus_tag="IVO69_001705"
     CDS             complement(4636..5967)
                     /locus_tag="IVO69_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400482.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIALAGPVVI
                     LVYLICGVAIYGLMRAMGDLFLANSKFNSISDFIDYYLGEKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIV
                     FFILLGIGLSVSYFHTQSIGIKANFGQLNELLPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYVGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQSGAAIVNLMILVSSLSSANSAVYSTSRILYQTAKEKSLPPWFCALSKKGVPLHGIL
                     ITGTLFLAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHIQFVKEKKRNTIP
                     KYKDLALLVFFVAIAFSLLFERMTRFIPFLLVIWFVLLSFVYKRISDRNIN"
     gene            complement(5933..7411)
                     /locus_tag="IVO69_001706"
     CDS             complement(5933..7411)
                     /locus_tag="IVO69_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400483.1"
                     /translation="MKDAGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFIVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLTFDMAKIGGKIGVWLGLY
                     VPLTMMLLLGLAAWIKVGIVPTGTLGNFSWDKVIPDSTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGISVLRLRY
                     THAKLNRPFRIGKKENKSNLGVWAIVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7718..9553)
                     /gene="tdc"
                     /locus_tag="IVO69_001707"
     CDS             complement(7718..9553)
                     /gene="tdc"
                     /locus_tag="IVO69_001707"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7400484.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMADGWGHITADGSIANLEGLW
                     YARCIKSIPLAVKEILPEKVKNMSEWALLNLSVEEILEMTESFTDEEMDKVKAASSRR
                     GKNIQKLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     DQKIPILGVVAVVGTTEEGQVDSVDKIIQLREKLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHYVTIDKEVYEGFRAISEADSITIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVEAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLEQLHFTVKGKTIEVYPLNHPDFNMVNWVFKVQDC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFIERMGLSKEE
                     WQKEQQITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIK"
     gene            10023..11450
                     /locus_tag="IVO69_001708"
     CDS             10023..11450
                     /locus_tag="IVO69_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7400485.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIVYTHREKDCYTLYFSEHIPRCKIMQQLTQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRL
                     GSFKSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQLGTVTID
                     PTFIEEIKKRPIYNYVESTWETTDFQTYYKKEEFVFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFETHFRQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQANLYKDVQTLFSSWAKRLPYDLRFNPNCMRAFVIELSGILRLTKERLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYASISDIKKYAQQPSNRVLCERTLYTPDA
                     VQYENIIPISINTIERAIISAVQNK"
     gene            complement(11611..11976)
                     /locus_tag="IVO69_001709"
     CDS             complement(11611..11976)
                     /locus_tag="IVO69_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7400486.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLF
                     VHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVPNK"
     gene            complement(11973..12338)
                     /locus_tag="IVO69_001710"
     CDS             complement(11973..12338)
                     /locus_tag="IVO69_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7400487.1"
                     /translation="MFHSNLIEFLEWIIAILNICSIGILVAGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEIG"
     gene            12779..12907
                     /locus_tag="IVO69_001711"
     CDS             12779..12907
                     /locus_tag="IVO69_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7400488.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12964..14658
                     /locus_tag="IVO69_001712"
     CDS             12964..14658
                     /locus_tag="IVO69_001712"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7400489.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKDTPMQDEITVTGLKMTNTLTQ
                     EEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(15062..15922)
                     /locus_tag="IVO69_001713"
     CDS             complement(15062..15922)
                     /locus_tag="IVO69_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400490.1"
                     /translation="MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQN
                     MQSDYAKKVAAEINREIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVE
                     LIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFID
                     QILTIRERMVSFLPMISSILLVLLSGVFYLYRMSWKKVLWSFAVVIGGAGFQLLSLSV
                     SLYLYDMASQLDIRTQALEHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEK
                     KVFSKRNGYK"
     gene            16213..17346
                     /locus_tag="IVO69_001714"
     CDS             16213..17346
                     /locus_tag="IVO69_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7400491.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDETNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP"
     gene            17377..17691
                     /locus_tag="IVO69_001715"
     CDS             17377..17691
                     /locus_tag="IVO69_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400492.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAVFLTIRPILRIVRNIFIYDEKRTRKKHGK
                     AV"
     gene            complement(17828..18286)
                     /locus_tag="IVO69_001716"
     CDS             complement(17828..18286)
                     /locus_tag="IVO69_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400493.1"
                     /translation="MERRTVIKLRDRPTLIKETAEWFASKWHLPVAVYEESIQASTRK
                     NTSVPQWYVVLNEWGEIIAGAGVVDNDFHDRVDLTPNVCALFVEKDYRNQRIAKMILE
                     TIKQDFAEMGVKVLYLVTDHTDFYEKYNWQFIGMAQDNEGIPVRVYQMTL"
     gene            complement(18508..>18913)
                     /locus_tag="IVO69_001717"
     CDS             complement(18508..>18913)
                     /locus_tag="IVO69_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400494.1"
                     /translation="ATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPI
                     VLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPVIGGVLATKSIAGGV
                     LAAVNLILSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         5876 a         3878 c         3267 g         5892 t
ORIGIN      
        1 aaatggactt tgtctacagt ctgaagctgt cctcacagac agcttttgtc tacagtctgg
       61 ttcatctagt ttttctattt tacgtagatc aacagagatc caatgttctt tattcatgtg
      121 ataggctgga aaatacccct cttttcctcg caaaattccg ccaagttcag gatcgacctt
      181 caaatcaagg atgtcgatga tcctatggtc acttgcatca tcatagattt tttcttctgg
      241 aacagacatc aggaccccaa accatttttt gcttttttga tgtctgaata cgcaataatc
      301 aggaaatttt ttaaaaatat attctggaga caaattgtat gtttcatcga tgtgatcgat
      361 tagttctttt ttcgttagca tctgcttcca tctctttctt attgattcac tttattcaaa
      421 gataccacgt aagaatagaa aaatgaatac tggttgaaag atcagttgaa acttgaccac
      481 cgtttatttt ctccgtattc ttagaataga agggagtctg atttatgtat tcactaatga
      541 aaaaaatcat taccgaaaaa gatattcgac gtcatgtttc tttagtcgaa caattgctga
      601 atcatacaaa gataacggta aatgaattag ctgaaatcat agggacaacc gaacgaacga
      661 ttttttctga ccttcaatct attcgctctc agctgcccga aggctgggac atcctcagcg
      721 atcaagcagg aataagtttg caaaaccatc aaaatttatt aacaaatgac ctttgggaga
      781 tttttttcaa acaatcagtc agtgttgagc tgctgaaaaa tctgcttttt acaaaaaaag
      841 ttgctgtccc tgattttcta gcggatcacg ggctttctta tgggacatta aaaagacatg
      901 tgactaaaat caatcagcgt ttagcaagtt acgacttgca gatcgatttg acgaaatata
      961 cagcttgtat tcttggaaaa gagcgtgcga ttcgtacttt ctatcatcga ttgctgattc
     1021 ctttcactca taacaactat tttttcgagg attattcgat ccatgaatcc cattattttc
     1081 aatttttaag aaacctcagt caaactgaac tagctgttga aacagaagag attttcggaa
     1141 catgttggtt ctttatcaat acgatccgta taaaagcgaa ttgccgatta gattcttcta
     1201 tccatatcaa cagcacactc tcctccctgt atgactctgc attgaaaaaa ttgtatttaa
     1261 cggaaggtat ttatcttaaa gacactgagc tttcttttgc ttctttctgc tttctggaaa
     1321 gttggaatta caacaataac tatggacaag aaatcgctcg ctgcctgcat cattctccat
     1381 ttttagaggt cctcgaaacg tttgtagagg aactagcttc agagctgtct cttgatcaac
     1441 taaaaaaaac atctctgact gataacttgg ctctcctcgt attaaaatat catgaatcgc
     1501 cgatactgat ttaacctttg gaacgccaat accatcgttt tttagagacg tatgatcagc
     1561 aatatgcaca cttgtatcag cagaaagaga atttactgaa cgatctaaaa aaagaaatgg
     1621 aaatcaatga accaaattac tttcttcaac tcttgtcttt actgatccaa caaacgattt
     1681 tttctatcaa accgaatttg ttcaatgtct attttatttt ccaaggagaa ccttcatgga
     1741 aggcgttctt gcagcaggaa ttaaaagatt acttgggaaa acgtgtctgc ttcttaccta
     1801 tcgaactaac cgatctttcc actatttctt ttgaaaaaac agatatcttg atttccaatt
     1861 tccctctgga ttctctagat atccctattg tatacatttc ttctattcca acgaaaaatg
     1921 agctcaatcg tttgactgaa ctcactttca aaactttttt atagtaacgg ctagcgactg
     1981 aattctctag ctgttttttc ttttttgcga aacagtttat tcatgatccc tttttctatt
     2041 tctacaacta agaatagaag aatcacattt gccccaatca ataactgttg tgtcatcgtt
     2101 aaacttactg taccgatcag ctgctggccg cttggcagaa aaataaccat cccctgcaat
     2161 aaagcaagga tacccaatga aagaaaaagc gatttgtttt gcaaaagacc ttggttcaac
     2221 gacggcttgg acaactctct gcaattgatc atgtaaacag cttgagctaa gacgatattt
     2281 tgcagaagaa tggtttgctg taagctttgc ccttcaaatt gcatagccaa taaataggca
     2341 ggaaccatga tcagtagtgc gacatagatg atccgaaaca agtgataacc tgataggatt
     2401 ccttgctttg gatctcttgg tggacgcttc atgatttctg gatctggcga ctcgaaccct
     2461 aatgcataag ataaagtgat tgtcgttacc atattgaccc ataaaatctg tactggggac
     2521 aaaggtagcg gccgattcag tatcagcgcc aaaatcaaga tcaatccttg agctaacgca
     2581 gttggtaaga agaaattgat ggtcttttgc aagttatcca aaatgcgccg accttctttt
     2641 acggcttttg cgatcgtatg gaaattatca tctcctaaaa ccatatcagc tgcctgtttg
     2701 cttacttcac tacctttgat tcccatagcg attcctatat cggctctttt caaagcaggt
     2761 gcatcattta cgccgtctcc ggtcattccg acgatttctc catttttctg caaagctttc
     2821 acgattcgca gtttgtgttc tggtgtcgtt cgagcaaaga catcgacttt tggtacttgt
     2881 cgaatcaact cttcttcaga cataagatcc aaatcgatcc cttctaaaac tttagcagta
     2941 tgttttaagc caatttgttc cgcaatcgct tttgctgtgt ctttatgatc ccccgtaatc
     3001 attttcactg agatccctgc ttgttggcat tcctttacag ccttgactgc actttctttt
     3061 ggcggatcga tcaacccggc aatccctgca aaagttaatt gggttaaagt ctcatgcgtg
     3121 agttcctgtt tcgaatctac ttttttgtaa gcaaatccta aaacacgctg accttttttc
     3181 gcaaacgctg tggctgtttg accccatgct tcttgttctg aagaagaaag agttgattta
     3241 gccaataata cttctggcgc acctttgacg taaatcaccg ctccttttcc ctccgtatgc
     3301 atcgttgcca tatatttgta ttcagagctg aacggaatct ttgcttctat ttttttcaaa
     3361 ggcagattgg ctgtttcagt gtgtcgaatc agtgcaagtt ctgtcggatt gccacttaaa
     3421 ttttctgttt tctgttcttc tttcgtcttt aactcctgac aatttttcat attagacagt
     3481 acgcgagttt cttcctttgc tgctacatcc acaactgtca tttcattttt tgtcaatgtc
     3541 cctgtcttat ctgaacagat cactgtcata gaacctaatg tttctacaga aggcagtcct
     3601 ttgatgattg ccttttcttt tgccatttca tttactccga cagataggat catggtcaaa
     3661 acagctggca atccttcggg taccattgac accaccaaag cgatcatagc tgaaagaagg
     3721 ataggccaat ccatgccata gctgaatgtt gtaaaaacaa ctaagaacaa gatcaaacaa
     3781 agaatgcctt gaaaaatttg tttgttcaat cggttgatct tcttgatcag aggcgtcgtt
     3841 tgttgttcga ccgattgcag tgcttgattg attttaccga tttctgtcgt gtttcctgtt
     3901 tctacaacga caccgactgc agaaccagat tgtacaagtg ttcctgaaaa agccatattt
     3961 ttctggtcac cgattgattc ttctgctgcc aaaacagtat gatttttctc agatgcttct
     4021 gattctcccg tcaaaatcga ttcttccact agtagatcat gtacttcgat caaccgaaca
     4081 tctgctggaa caacgtctcc agcacgtaag acaatgacat ctccttttac tagttctttc
     4141 gcaggaattt tttcttgcat cccgttcgat agtactatcg cttcttgtcc catcagctgc
     4201 ttcaaaccat tcaacgattc ttctgctttt ctttcttgcc aataactaac gagtccgttg
     4261 acgatcacta cgaataaaat aatacttcct tcgatgtatt cgctcgaagc aaatttcaaa
     4321 aaagaggcaa tgatcaacac gatcatcagc agatcagtaa agtgctttac tatttttttc
     4381 catcgttttg tctgctgtct tgcttctatc tcattatatc cttgatcagt caggcgtttc
     4441 tgccgttctt cttgcgacaa gccttctgtt gatgtttgat agtttttcaa tgtatcttct
     4501 accgtcaatt tataccagtt catcctatcc cctcctactt aattgatact tttattatac
     4561 gtagggagat ctttcaggcg tatcgattat ttcagagaag tctgaaataa ttcgttttgc
     4621 cggatatatt tagtcttaat tgatatttct atcgcttatt cttttatata caaaacttaa
     4681 gagaacaaac cagataacta ataaaaaagg aataaaacgt gtcattcttt cgaataacaa
     4741 gctgaaagca atggccacaa aaaataccaa caaagcaagg tctttatatt ttggtatagt
     4801 gttccgtttc ttttctttaa caaattgaat atgtgcacat aagatcgaag accacacaaa
     4861 tagaaaacaa ctcgttgtca tacctgccaa aagctgaaaa aacaatcgac tatcagcgac
     4921 aaaaaaatgt agacaaagac ctgcaagaaa taacgttcct gtaatcaaaa taccgtgtaa
     4981 aggtacgcct ttcttagaaa gcgcacaaaa ccaagggggc agactttttt cttttgctgt
     5041 ttgatataag atcctgctag tactgtaaac cgcactattt gccgaagaca aagaagaaac
     5101 cagaatcatc agattgacga ttgctgcccc gctttggcaa tggatcattt gcaacatagt
     5161 gactaaagga ctttctttta aatctaaaca atgccatgga acaacgaata aaatcgacag
     5221 aatcgttcct acatagaaaa agaggatacg ccaaggcaga cagttgattg catttttcaa
     5281 attcttttgt gggtcactcg tctcaccaac tgttaatccg atcatttcaa ttccagcaaa
     5341 actgaataaa accataggaa atgaagcaat cattccagca aagccaaacg ggagtaattc
     5401 attgagctgt ccaaaatttg ctttgattcc gatagactgg gtatgaaaat atgagacgct
     5461 caaacctata cccaataaaa taaaaaaaac aatccccaaa atcttgatga ttgagaaaaa
     5521 agattccaat tcgccaaacc atttgacggc aacaagattg actgccatta agatcattaa
     5581 tgcactacct ccgcttatca aagctggtac atgtacaaac cagaaatgga tatacaatcc
     5641 tattgctgtc aattccgcaa gtccagctcc aagccagcaa agccaataag tccaattgac
     5701 aaaaaaggcc cacttttcac ccaaatagta gtcaatgaaa tcactgatag agttaaattt
     5761 actatttgct aaaaacaaat cacccatagc ccgcatcaag ccataaatag ccacaccgca
     5821 tatcaaatag acgagaataa caactggtcc tgccagtgca attgcctttg ctgaacctaa
     5881 gaataatcct gttccaattg cgccacctag tgcgattaac tggatatttc gattagttag
     5941 agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca agcagcatgg
     6001 cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acatagtggt actacaaaca
     6061 aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct ttcatcgtca
     6121 gtccgacaga aaagccgatt gccgcaaata aaacaataac gatcgcccag acgccaagat
     6181 tgcttttgtt ctcttttttt ccaattctga atggacggtt caactttgca tgtgtataac
     6241 gtaatctcaa aaccgagatc cccataatga catagacgat acaatagatt acagtagttg
     6301 aattaactag catcaaaaat gcttgattga cgccaggaat cagcaaataa aaaagtgcaa
     6361 agattgagat aatgacagat tgagtcaaaa taacggattt ggatacgtca aattgatttg
     6421 tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct gtcactgtct
     6481 ttgcaggtcc tgatgcccaa gcagataatt ggacggccac accgatgaag acaagcaaac
     6541 taaacagatt cacaataaga tgcggcaaac cgagaatttg gcaataaatc gaaatagatt
     6601 gtgcgatatt attcaattcc atttgacctt ttggtacgac atttgctaca actaaagcat
     6661 tcaaaatatt gacgaaaaat ataaaaatca gagcagcaaa tactccgcga atatacgttt
     6721 tcactgggtt cttcaatcga gtgatataca cggaggacat ttcgatgcca gtaaaaataa
     6781 acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt actatctggt attactttgt
     6841 cccatgaaaa attgcctaat gttccggttg gtacgattcc aactttgatc cacgcagcaa
     6901 gccctaataa gagcatcatc gtcaaaggca catatagacc taaccaaaca ccgatttttc
     6961 ctccgatttt tgccatgtcg aatgttaaat tcaaaaaagt gatgatccaa taaacaacta
     7021 aaataacaac taatgtaaat ttgctgttca gcccaagcgg cacattatcg ataatattac
     7081 caaacaacgg agcgagtgct gaagcaacca tcaccatccc tggaaacatt tggacccata
     7141 acagccagga gacaatgaat ccccattttt ttcctagagc attcgatacc caaagttctg
     7201 gaccaccttt ttgaggaaac attccggcaa attcaccaga gatcaatgag atcggaaagg
     7261 catataacaa tacggcaacc agcatgtaaa aaatcatcgt ccagctcgtt gctgccacat
     7321 ctggaatatt tcgtacactt gctacaaggg cacaggtcat cccgataaag gtcatggtcg
     7381 tgatttgttt tgatgaacca gcatctttca tctttattcc ttctttcttt caatagcgac
     7441 atgtttttcg ttttctttta tttttgttca ataacaaaag tgacaaggct tcatcgattc
     7501 agtacaaaac ccagtatcac cgaaacatcc attgaacgaa cttagtgaac tgggtttgat
     7561 acgtgaaacg aacgaaccaa ctatagaaag atttataaaa aagaatgtct tgctgaggaa
     7621 tatatgaaaa acagggggaa atacaactgt tttgatgagg caaataattg tctgttctcc
     7681 atatgtctct tgctattctt tcacacaaaa atcagttcta tttgatgatt tcgtttagtt
     7741 tcttttccat tgctttggcg atttcttttg tataaaaatt gaaaattcga tcatcattca
     7801 aataaggagt catgatagcc gcacgtagca gagtgatttg ctgttctttt tgccattctt
     7861 ctttcgatag acccattctt tcaataaaac ggatcggaga atcaccgtaa tcttcttgtg
     7921 taaatgtcgt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
     7981 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta caatcttgta
     8041 ctttaaatac ccaattgacc atattaaaat caggatgatt cagtggatag acttcgatcg
     8101 tttttccttt tacagtaaag tgtaattgtt ctaaaaattc tctgaatctt tgagctgctt
     8161 cgattgatgc accaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgag
     8221 ctgtccagac tgcagcagcc gtcgctcctg cttttgagcc ttctaaaata taagcaccta
     8281 gcatatctgg tgcttcgact gatttttcaa acacatatgg tgcaaaataa gaaatgatat
     8341 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
     8401 tatgcggatc aatcgtgata gaatccgctt ctgaaatagc tctgaatcct tcatacacct
     8461 ctttgtcgat cgttacgtaa tggtggaata catgatgctc ttcaaagaat tcagctaagg
     8521 aagcatatgg cacaaattct cctgcttcgt ttaaaaacag tgaacgcgcg tacccgccat
     8581 atgcagcgtc aacatgtaaa tagaaataga taccttcatc ttttaacttt tctcttaact
     8641 ggatgatttt atctacactg tctacttgtc cttcttctgt tgttcctacg acagcaacca
     8701 cacctagaat cgggattttt tgatcagcta attcacgaat tgttttttcc aaagcgttga
     8761 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag ccgactccgc
     8821 aaatatctaa cgccttcatc caagaatagt gttttgtttg aggaaccagc catttcccta
     8881 atttttggat attctttcct ctacgagaag aagcggcttt gaccttatcc atctcttcat
     8941 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga cagattcaaa agagcccact
     9001 cagacatgtt tttcactttt tcaggcagaa tttctttgac agctaaagga atcgatttga
     9061 tgcaacgggc gtaccataat ccttcaaggt tagcaataga accatctgct gtaatatgtc
     9121 cccatccgtc tgccatgttg aatagtgagg caaaatcctg tcctacttct gcttccatct
     9181 gcgaagttgc catagaagat tctagtgcaa cgttattggg attccatagc atcgcataat
     9241 tgtaagctaa taaagcaggc atcagtgttt cagcgttcat ttgtccccag taacggcctg
     9301 ctgaatgcca ggggatcgat cctgcgcgta atcgctgact gacttcatcc aagacttcta
     9361 tcatatggtc gcgggtggct agataatctg gagttagctt atctccttcg ctaattgcag
     9421 gcatatctga aggaatataa ttttctctcc agccaagatg ttcatctacc atcttgttca
     9481 acaacatttt gtaaactggt ccattttcag ctttgtcgcc tataaagact gccttgatat
     9541 ccatatcttt catgtttctt ccctccatat ttcgttggtc aatgtctcgc agatacatgt
     9601 gcttatctgt aacacctctt cactatagcg ccaaaaatga gaaaattttt tttataacat
     9661 cagattcacg tagaatattt caaacaaaaa taaataaagt cggatttttc tgataattga
     9721 acgtggaaaa acgaacaatt ttttaataaa tcagacttct aaaatggata gtctaaaaaa
     9781 aacgaacaaa taacttaaat gaccatcatt cattcatcaa ctgtatttgc attccttttg
     9841 cttgttagaa attcacaatc tattttctaa tgaaaatatt tatttttcaa tttttataac
     9901 ataaaaatct ttcattaacg gaatttacag gatttttcag aaattaattg atgatttcca
     9961 aaagaaaaca tgtcataata ttttccatat tgtgcaaaaa aatacaagat ggaggcttta
    10021 ctatgattga agaatatatt gagaagaata ttttgagaca gctgttttta tgtggacaat
    10081 tctatgtaaa taaagaagtc aatctagaaa agctctccaa tcttctacat gtttgcaaaa
    10141 ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacaa attgtttata
    10201 cacatcggga aaaggattgt tacactttat atttttcaga acatatacct cgatgtaaga
    10261 tcatgcagca attaacccaa aattcgttgt ttttaaaaac ttgcctgctt tatttggaag
    10321 aagatgaacc tgattatctc caattaactg agtgtgaatt catctctgtc agcaaagctt
    10381 actccttaaa aaaacaagta ttggcttatt ttcatgattg tgggatcgaa atcgaccgct
    10441 actcccctcg attcactgaa atggaaagac gtctgttgct tcttaatgtg gcttatcgtt
    10501 taggcagctt taagtcctgg gagctacctg aatcgttttt tgaacgtgcg gatcgtttca
    10561 ttgaatctgt aacggagaat agcggtagat tttatgataa agaaaataaa gaaatcttat
    10621 cgattggttt tgctattagt ttcttgcgcc aacagttggg tactgtgaca atagatccaa
    10681 catttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgagtca acatgggaaa
    10741 ccactgattt ccaaacctat tataaaaaag aagaatttgt ctttattttg acactgttca
    10801 atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagatttt caacagctac
    10861 accaagtgtt tattgataat actcccgaaa taaaagaact agttgctacc tttgaaactc
    10921 atttccgtca agaattattc gggaatcagc catttgaacg tgcactgatt catctgatgc
    10981 gttcggcttg ggataactat caattattca tgccggaaaa attttatctt ctaaatgagg
    11041 aacaggcaaa tttatacaaa gacgtccaaa cacttttttc cagttgggcc aaacggctac
    11101 cttatgatct acggtttaat cctaattgca tgcgagcttt tgtcatcgaa ttgagcggaa
    11161 ttctccgtct gacaaaagaa cgtctgacta tctatatcgt cacaaattcg gatgttcatt
    11221 atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa gttgcaccaa
    11281 caatctatgc aagtatatct gatataaaga agtatgcaca gcaaccctcg aaccgagtgt
    11341 tatgtgaacg tacattatat acaccggatg cagtccaata tgagaatatt attcctattt
    11401 caatcaatac gatcgaacga gcaatcattt cagctgtgca aaataaataa gaggctgtga
    11461 taaacatcat gtcacagcct cttattctga ataaacaata ttccaaaagt aacttcttcg
    11521 gaaataagcc gaaattttca taaatttctt cttattcctt gaatctgccc acttcttaac
    11581 aacctctcga taaacggcgg gctcatagcc tcacttattt gggacgtcta ctggcccgat
    11641 ttctttattt aaaaaataag aaatgatcgt tcggataact accaatgcag ctagctggaa
    11701 aatatcctgc aaagtaggtt tgataatcga ttcgatgatc ccagccgaaa caagaatttc
    11761 taaacttagg agtatataac ctcctaacat ctttttaata gcattgttct cgctgactcg
    11821 gtcgtttttc gcttctgtat ggacaaataa gtatttcaag aaatccttaa tcgacagaca
    11881 aacgccccaa atcaataaac agatagcaaa aatatcaagt cccagtacaa gtttttcaaa
    11941 atatggaaga agaaactgca tcaactctgt cgtcatccta tctctcctct tctttgtttc
    12001 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
    12061 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
    12121 acgactagca cttccaagct taacaaaata taacttgcta acagttttct tgtatctgca
    12181 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
    12241 agaacaagaa cgatcccagc aactaatata ccgatagaac aaatatttaa aatagcaatg
    12301 atccattcta gaaattcaat caaatttgag tgaaacatat aagctaccta ctttcttttg
    12361 atttgttcct attcttttat tgtaaaagaa agagaccttg ttcgtctatt taaaagttgt
    12421 tttattgatc acaccttgcc taaaatcctc ctcttccctt tctcattctt caccctccat
    12481 accatccata catcatggaa gtatccaaaa caacatagca agttaaaata aggcttagtc
    12541 cgtcatcagc ttttcaaagt ggcactgtct cgcgcttttt ttacccaaat ttctaaaaat
    12601 agaaccatac atctcctcaa aacttttctt ctttcctacc ctattactaa ttacaggaaa
    12661 atcagacact tcttcttttt tgtcaaaaat tcctttttct tctcacccta tcatttggca
    12721 ttctcataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
    12781 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
    12841 tctaggaaaa aaaacagggc tattcgaaga tgacagtcat ttatatgatg aatttgaatc
    12901 catctagcat cttatttcgt tgaatcacta cggacatact tggaagacat tggaggaatc
    12961 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
    13021 gtttatttga ttcaagaagg caaatggaaa ggggaccaga tcacctttta ttcattggat
    13081 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
    13141 aatcatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
    13201 cgtacctatg tagatttgat ggatctatta agtcgaatac cttctgttac tgagccagga
    13261 atgactgctg aagaagatac gagagatttt gatgcaaaac accgtacctt tgacaaagca
    13321 cgtttgatgg aaggtggtaa agggatcatt gacggcggaa aactgggctt caacaacaag
    13381 gaccgtattc ttctgactaa actgattgcc atgcctgatt cagaagaaga aaaactagac
    13441 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
    13501 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
    13561 tatatgcatc aaatgattta tgaattcact caaatcgaac atttagtggg cgtcaatcga
    13621 acacgttaca atcagtacga aagtatcatg cttcctttga tcgagtactt gaaagaacaa
    13681 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga tacaccgatg
    13741 caagatgaaa tcactgtcac tggtttgaaa atgactaata cattgactca ggaagaggaa
    13801 catgtcacgg tagatgaaga tacagcggtc attttcacga atggttccat tacagattca
    13861 gctactttgg gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
    13921 agtctctgga aaaaagccag cgaacgtttc tataacttag gaaatcccga caaatttttc
    13981 gcggatcgag atgcgagcga atgggtcagc tttacactga cgacgaaaaa ccacctgtta
    14041 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
    14101 tcaactacac cgatcacgcc attaggccaa aaagatgtca atatgtctat cgtcgtgcat
    14161 catcaaccgc attttacgtc tcaaaaaccg aatgaaacag ttatttgggg ttatttcctt
    14221 tatccaagac gacgtggcga atttgtggat aaacagtata ttaagatgaa cggaaaagaa
    14281 atgttggaag aactgatcgg acaactttca aaagtagatc ctggtccagt gaatattcgg
    14341 gaaaaagaaa cagagatttt cgacagcgta ataaataata tccctgtcta tatgccttat
    14401 gcttccgcat tatttaataa ccgtgcaaaa agtgatcgac caaaagtcat tcctaagcat
    14461 tcaaccaatc tggcctttac aggagaattt gttgagcagc cttatcagat gatctttact
    14521 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg catccctatg
    14581 tcccgtcttg tcaaaacacc gcgttacgac aaagacatcc ctactctagc tcgtgccgca
    14641 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccttgcatca agtaataaga
    14701 acagccaagc aaaaacagct acttatgttt ttgacttggt tggacttatt atcgcagatg
    14761 caagcttttg aacaccgttt atcaagaggt tgtgaaaaag acgtttaatt tcgagtatta
    14821 agaaaaattt tgtgaaaatg gttcctcaca tttttgcata atttggctta ataccgagaa
    14881 actggctttt gaacaccgtt taccaagagg ttgtgaacct accgtttagc tctgagtcac
    14941 aagaagcaat ttcaaaaaat agcttcctat gttttatgaa tattgtgacc gaggagttgg
    15001 taggggaacc ccatttattc ataacaagag gttgtgacag atttcatgtc acaacctctt
    15061 attatttata tccgtttctt tttgaaaaaa cctttttttc agtattcact ctcttgccaa
    15121 tccaccataa tccagctgct atcactactt caacgattgc tactacacta aaaaattgga
    15181 tgaatgacaa aatatatgtg tctaacaaat gttcgagtgc ttgggttcga atatctaact
    15241 gactggccat atcgtacaaa tagagactga ctgacagact taataattgg aagcccgcac
    15301 ctcctatcac cactgcaaat gaccaaagaa cttttttcca agacattctg tataaataaa
    15361 acactccgct cagcaataca agcaagatac tactgatcat tggaagaaaa gaaaccattc
    15421 gctctcgtat cgtcaatatc tgatcgatga acgacatcaa atagattgat tgtattcctc
    15481 gtcgaaaaat cccagaagtc atatagatga gtaaattgtt ttcttcacta gcaaatggat
    15541 catcctctgc ttcttcttgt ttgtatgtat tgattatctc ttggagcttt tcttctactt
    15601 ccttttctcc aatcagttct actggttctt tttcatatct gttgcggatg aaaagctcga
    15661 tatttttttg tactagttct tcagaaacaa tcttttccaa tgcttcagcc ggagctccga
    15721 tctcataatg atagctttgg atctctcgat tgatctcagc agctactttt ttcgcatagt
    15781 ccgattgcat gttttgctct aacataaaat gttgatttgc caaggtgctt attaaacaaa
    15841 gcataacagt caggcaaaac aggcagacag aactaatcat aacaatgatc cgtttattcc
    15901 atttattttg aatttgtttc attacgcctc ctcgtatttg ttttttcact gaaacgaatg
    15961 atggttggtt attgtttaaa tagttaaaat gattcaatag ttcaactata ctttactgta
    16021 cgtaaggata caatgaaggc acactccttc tttttctaat aaaaaaaaat tttggaactc
    16081 ttgctttttt gaggattttt attttgagaa aaaagcgttt cattatcaaa aagataagaa
    16141 ctcctttaga atgaaactat ctgtagtacc aataatgcag acaattttgt gaatctgaaa
    16201 ggagcatctc caatgaaagc agtcacttgg caaggaaaac aaaaaatgga aatcagaaac
    16261 gtagatgatc caagaatatt agaaccaaca gacgcaatca tccgtatcac agcaaccgcc
    16321 atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat
    16381 gttgtaggtc acgaacctat gggaatcgtt gaagaagtag gatcacaagt tcaaacattg
    16441 aaaaaagggc aacgagtggt tatccctttt aatatcagtt gtggtcattg tcatttctgc
    16501 ctgaatcaca tggaaagcca atgtgatgaa acaaatccag atcagttata tggcgggctt
    16561 ttcggttttg gcaagttgaa tggcaatcat tggggtgggc aagcagaata cctaagagta
    16621 ccttatgcag actctacgtc gtttgtcgtt ccagataata atcttcctga tgaaaaagtt
    16681 ttgtttttat cagatatttt gcctacagct tattggagcg ttgaaaatgc aggcgtaaaa
    16741 aaaggcgata cagttgtgat tctaggatct ggtccagtcg gtctatttgc tcaaaaattc
    16801 gctgtaatgg ctggagcaga gcgcgtgatt gcagtggatc cagtacaaca tcgtttagat
    16861 aaagcggcta attacaacgg cgtggaaact tatttcttag aagacacagc acaagctgga
    16921 acggaattgt atgagttggc taaaggtggt gccgatgtca tcatcgattg tgtaggaatg
    16981 gatgggttag aacctgtaaa agaaaaagca aaaaatttgg tgagcctaca aagcggcacg
    17041 atttctccat tacagatggc ttcgcaagct gtcaaaaaat tcggaaccgt ccaagtaact
    17101 ggtgtgtata tgacgccagc ttcttcttac ccgcttcaag agtttttcat gcgagatatt
    17161 gcggtcaagc atggacaggc accaattatc catctgatgc cgaaaatcta cgaaatgatt
    17221 gcagaaggac tatttgatcc tacacagatc attactcatt cgctgccact agaagaagca
    17281 gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa
    17341 ccttgaaatc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc
    17401 catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc
    17461 tatttccaaa accgttacac attgatctca ctagctgtcg acttattaac cgggattttc
    17521 tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt
    17581 tatttggctg gtgctgtttt cttgacgatt cgtcctattc tcagaatcgt taggaacatt
    17641 ttcatttacg acgagaaaag aacaaggaaa aagcatggga aggcagttta gccttctcca
    17701 tgcttttttg ttattcttgt gttgtgcata tttcgcatac tgcgattcga aaaatttcta
    17761 agtatagaac aatttttcgg tttccttctc tatatactca cgtctatgct gtttcatgta
    17821 tctactctta cagcgtcatt tggtacactc gcacaggaat tccttcatta tcctgagcca
    17881 tcccgataaa ctgccaattg tatttttcat aaaaatccgt atgatccgtc acaaggtaaa
    17941 gcacctttac gcccatttct gcaaagtctt gcttgatcgt ttctaaaatc attttggcaa
    18001 tccgttggtt tcggtagtct ttttcaacaa acagcgcaca gacattcgga gtcaaatcta
    18061 ctcgatcatg aaaatcattg tcaacaacac ctgcacctgc aataatctct ccccattcat
    18121 tcaacaccac ataccattgc ggaaccgatg tgttttttct cgtgcttgct tggatacttt
    18181 cttcataaac agcaactggc agatgccatt ttgaagcaaa ccattcggct gtttctttta
    18241 tcaatgtcgg tcgatcccgc aattttatga ctgttcttct ctccatttgt actctctccc
    18301 attcatattt ggtctattat agcaccttgt atttcaaata gctttccaat cgctcgattt
    18361 ttttgaatga atgggatttt ttatttatac acaaatttca agacaaatat acctcgattt
    18421 tctcattcat tattcgatcc taagttttaa attacttatt tccataaaaa aacagaacca
    18481 gataaccggt tctgctttct tttttcatta agcatccaaa gcttcttttc ttctttcttg
    18541 gatcgtagct accttgacga acggcagata gatcaaaata gaaaggatca gattgacagc
    18601 ggccagtaca ccacctgcaa tactcttcgt tgccaataca ccgccaataa ctggtggtgt
    18661 tacccatggt ggcataacag tagctgctgg cactaaacct gtagaagtcg caaagtacgc
    18721 aacactaact aagaccattg gtgtcaaaat aaatggaatg aacatgattg gattcaatac
    18781 gatcggtaaa ccaaacatcg ttggttcatt gatattgaaa acacctggcg caatcgacaa
    18841 gtttgttaca accatatacg gtttgttttt acgtcctact aaataaatag ctaacagtaa
    18901 acctaatgtt gca
//
LOCUS       DACCPY010000056        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCPY010000056 DACCPY010000000
VERSION     DACCPY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.057"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IVO69_001718"
     CDS             complement(21..251)
                     /locus_tag="IVO69_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400495.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IVO69_001719"
     CDS             complement(687..1253)
                     /locus_tag="IVO69_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7400496.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IVO69_001720"
     CDS             complement(1253..2209)
                     /locus_tag="IVO69_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7400497.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IVO69_001721"
     CDS             complement(2209..2865)
                     /locus_tag="IVO69_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7400498.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IVO69_001722"
     CDS             3235..5253
                     /locus_tag="IVO69_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7400499.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IVO69_001723"
     CDS             5680..8094
                     /locus_tag="IVO69_001723"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7400500.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IVO69_001724"
     CDS             8442..9995
                     /locus_tag="IVO69_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7400501.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IVO69_001725"
     CDS             complement(10376..12160)
                     /locus_tag="IVO69_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400502.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IVO69_001726"
     CDS             complement(12175..13896)
                     /locus_tag="IVO69_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400503.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IVO69_001727"
     CDS             13996..14757
                     /locus_tag="IVO69_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7400504.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IVO69_001728"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IVO69_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18840
                     /locus_tag="IVO69_001729"
     CDS             16664..>18840
                     /locus_tag="IVO69_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400505.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6186 a         3343 c         3771 g         5540 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
//
LOCUS       DACCPY010000057        18825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCPY010000057 DACCPY010000000
VERSION     DACCPY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.058"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVO69_001730"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVO69_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7400506.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IVO69_001731"
     CDS             complement(1551..2972)
                     /locus_tag="IVO69_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400507.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVO69_001732"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVO69_001732"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7400508.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IVO69_001733"
     CDS             complement(5383..6639)
                     /locus_tag="IVO69_001733"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7400509.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IVO69_001734"
     CDS             7219..8520
                     /locus_tag="IVO69_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7400510.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IVO69_001735"
     CDS             complement(8589..9188)
                     /locus_tag="IVO69_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7400511.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IVO69_001736"
     CDS             9442..10323
                     /locus_tag="IVO69_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400512.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IVO69_001737"
     CDS             10336..11946
                     /locus_tag="IVO69_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7400513.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12673)
                     /locus_tag="IVO69_001738"
     CDS             complement(12023..12673)
                     /locus_tag="IVO69_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7400514.1"
                     /translation="MCFICSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPNGEP
                     QLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTEICI
                     LRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLAKAT
                     GKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLEDL"
     gene            complement(12973..13638)
                     /locus_tag="IVO69_001739"
     CDS             complement(12973..13638)
                     /locus_tag="IVO69_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400515.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13785..14390)
                     /gene="dhaL"
                     /locus_tag="IVO69_001740"
     CDS             complement(13785..14390)
                     /gene="dhaL"
                     /locus_tag="IVO69_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7400516.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14418..15401)
                     /gene="dhaK"
                     /locus_tag="IVO69_001741"
     CDS             complement(14418..15401)
                     /gene="dhaK"
                     /locus_tag="IVO69_001741"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7400517.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15403..15798)
                     /gene="dhaM"
                     /locus_tag="IVO69_001742"
     CDS             complement(15403..15798)
                     /gene="dhaM"
                     /locus_tag="IVO69_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7400518.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15952..16272
                     /locus_tag="IVO69_001743"
     CDS             15952..16272
                     /locus_tag="IVO69_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7400519.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16384..17253)
                     /locus_tag="IVO69_001744"
     CDS             complement(16384..17253)
                     /locus_tag="IVO69_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7400520.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17349..18653)
                     /locus_tag="IVO69_001745"
     CDS             complement(17349..18653)
                     /locus_tag="IVO69_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400521.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         5364 a         3912 c         3440 g         6109 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaaggaac
    12661 aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac agctatttct
    12721 aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac
    12781 gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt
    12841 ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga
    12901 ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt
    12961 atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc
    13021 ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag
    13081 tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt
    13141 atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca
    13201 tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg
    13261 gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata
    13321 gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga
    13381 ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc
    13441 gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat
    13501 ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt
    13561 tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc
    13621 tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt
    13681 ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag
    13741 ccaaacaaaa aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc
    13801 acatctaaaa gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg
    13861 attgcacgct ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt
    13921 tcttttgcct ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact
    13981 ggcagccaaa catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata
    14041 cccgctgttc cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc
    14101 tcttttgtcg ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca
    14161 ccgactttcg atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga
    14221 tgatcggctt gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg
    14281 tctccatcgc caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc
    14341 tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc
    14401 ttttatcttg agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc
    14461 cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc
    14521 ataaaattac cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa
    14581 acatcattca tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt
    14641 acacctgctt cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt
    14701 gccagtactt tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca
    14761 acaccatact cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca
    14821 gttgctgctc gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt
    14881 tctttcaact cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca
    14941 aggacagtac ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata
    15001 tcatcatcta cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc
    15061 atatcgaagt tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca
    15121 ctatcggctt ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca
    15181 ccaagcaccg ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt
    15241 ccgcttcctc caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca
    15301 cgggaagatt ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt
    15361 tcttcgacaa tattgcttgg atcattcatg atttttttca atttattccc atcctttctt
    15421 tccggcattt tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat
    15481 ggctgctgca aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg
    15541 ctcttcttcc aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc
    15601 aaaaacaaaa taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg
    15661 gtctgatccg atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt
    15721 ttgcatctgc tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag
    15781 caaaatactt tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt
    15841 cgctttcatt aatgaacaat tcaagtatat aataaatttc taattttggg aaaatatgtg
    15901 tagtgcttct tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca
    15961 acaacaacga acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt
    16021 gaggtcatta ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt
    16081 ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc
    16141 gaaatggccg aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac
    16201 tatgaagtat taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc
    16261 tccacgagat aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg
    16321 gcactcaaga attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct
    16381 tttttatttc tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc
    16441 taaggaataa ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga
    16501 tttggatggc tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt
    16561 ttctcctgcc gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt
    16621 ttttgcttca aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg
    16681 actggccgct gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac
    16741 ctgatttaat tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg
    16801 gacagcttcc gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt
    16861 tcccacttta tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa
    16921 tttgtttttt gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg
    16981 caaatgatcg atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga
    17041 aatcgtttct cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc
    17101 gttttcagca ccagaagagc ttatggttgt tgaaggttct gtttgagcag gttcagaagt
    17161 ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa
    17221 cggcttcatt ctttttttca tcacttttcc catccttact actttttaac taatttctta
    17281 ttataagcaa aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg
    17341 tgcatctttt atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa
    17401 atcgttttga ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac
    17461 aaaagcatat atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca
    17521 atcagcgaag ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa
    17581 ctgaatgtaa aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt
    17641 aatccagcaa aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt
    17701 tgatcaatga agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg
    17761 aaattcgtca gccagacttc tgttccacct tttgcccaac gttttctgtg tcgatatagc
    17821 atcttcaacg tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac
    17881 cagtcattta actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg
    17941 aatccgccca catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca
    18001 taaagaccac cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat
    18061 tcaaccgttt gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg
    18121 accgcagcaa tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca
    18181 tctggctccg gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca
    18241 agtccgatat taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga
    18301 ttagcgtatt ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg
    18361 tctgtcacta atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta
    18421 tcttcgatca caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca
    18481 ctcaagggaa catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag
    18541 acaccaataa accatacaaa tccgcctaaa atcggataag aaacgagaaa aaaatacata
    18601 atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt
    18661 ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg
    18721 agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca
    18781 cttcttcaag taattcttgt ctttttttca atttttcatc ttcat
//
LOCUS       DACCPY010000058        18532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCPY010000058 DACCPY010000000
VERSION     DACCPY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.059"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..120
                     /locus_tag="IVO69_001746"
     CDS             <1..120
                     /locus_tag="IVO69_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7400522.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(444..527)
                     /locus_tag="IVO69_001747"
     tRNA            complement(444..527)
                     /locus_tag="IVO69_001747"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:cag)
     gene            1055..1555
                     /locus_tag="IVO69_001748"
     CDS             1055..1555
                     /locus_tag="IVO69_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400523.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1745..2089)
                     /locus_tag="IVO69_001749"
     CDS             complement(1745..2089)
                     /locus_tag="IVO69_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7400524.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2243..3163)
                     /locus_tag="IVO69_001750"
     CDS             complement(2243..3163)
                     /locus_tag="IVO69_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7400525.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3350..3841
                     /locus_tag="IVO69_001751"
     CDS             3350..3841
                     /locus_tag="IVO69_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7400526.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3865..6240)
                     /locus_tag="IVO69_001752"
     CDS             complement(3865..6240)
                     /locus_tag="IVO69_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7400527.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6566..7192
                     /locus_tag="IVO69_001753"
     CDS             6566..7192
                     /locus_tag="IVO69_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7400528.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7215..7919
                     /locus_tag="IVO69_001754"
     CDS             7215..7919
                     /locus_tag="IVO69_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7400529.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8111..8944
                     /locus_tag="IVO69_001755"
     CDS             8111..8944
                     /locus_tag="IVO69_001755"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7400530.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9141..11069
                     /locus_tag="IVO69_001756"
     CDS             9141..11069
                     /locus_tag="IVO69_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7400531.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11207..11662
                     /locus_tag="IVO69_001757"
     CDS             11207..11662
                     /locus_tag="IVO69_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7400532.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12132..12401
                     /locus_tag="IVO69_001758"
     CDS             12132..12401
                     /locus_tag="IVO69_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400533.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12801..13055
                     /locus_tag="IVO69_001759"
     CDS             12801..13055
                     /locus_tag="IVO69_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7400534.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13144..14190)
                     /gene="holA"
                     /locus_tag="IVO69_001760"
     CDS             complement(13144..14190)
                     /gene="holA"
                     /locus_tag="IVO69_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7400535.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14228..16501)
                     /locus_tag="IVO69_001761"
     CDS             complement(14228..16501)
                     /locus_tag="IVO69_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7400536.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16494..17033)
                     /locus_tag="IVO69_001762"
     CDS             complement(16494..17033)
                     /locus_tag="IVO69_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7400537.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17169..17387)
                     /locus_tag="IVO69_001763"
     CDS             complement(17169..17387)
                     /locus_tag="IVO69_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7400538.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17384..17629)
                     /locus_tag="IVO69_001764"
     CDS             complement(17384..17629)
                     /locus_tag="IVO69_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7400539.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17745..18473)
                     /locus_tag="IVO69_001765"
     CDS             complement(17745..18473)
                     /locus_tag="IVO69_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7400540.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5684 a         3566 c         3252 g         6030 t
ORIGIN      
        1 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
       61 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      121 gattcattat ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt
      181 ctttcgcatg acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc
      241 agtatttctt gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa
      301 ctgtcccacc ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa
      361 tttttcctat attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct
      421 tctctttata aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc
      481 tcactagcgc ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta
      541 gtttaggatt acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac
      601 gccctccctt atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg
      661 gttggtgaaa aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg
      721 ggattagtaa ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc
      781 tatcttcttt catttggata ttgattattt cttctgttga ttactatcct tctggaattc
      841 tagacgctga agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga
      901 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
      961 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
     1021 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
     1081 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
     1141 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
     1201 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
     1261 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
     1321 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
     1381 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
     1441 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
     1501 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
     1561 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
     1621 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
     1681 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
     1741 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
     1801 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
     1861 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     1921 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     1981 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     2041 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     2101 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
     2161 aagtaagatt ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt
     2221 attctaaaat aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata
     2281 cgccagttgt tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat
     2341 gagttggtaa aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat
     2401 catacatttc gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc
     2461 caccgacaga cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta
     2521 cctcttctgg cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg
     2581 catcaattac ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt
     2641 taaagaagaa gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga
     2701 ctgtttttcc agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa
     2761 agcccatttt taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat
     2821 tcatcaaaat attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac
     2881 atacaacatt cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata
     2941 caaacacagg gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc
     3001 attcatataa aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat
     3061 tggctggaat caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa
     3121 ccactatttt ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca
     3181 cctctcccct actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat
     3241 acttcgaact caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg
     3301 ataagataga tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga
     3361 tgctagcttc gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca
     3421 aacaacaaaa gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt
     3481 agagaacagc aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga
     3541 tgtattattt ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga
     3601 atcagaacac gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga
     3661 tcgtgaaaac gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc
     3721 acaaatccaa gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt
     3781 gattgcttta ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta
     3841 atcaaaaaac gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga
     3901 taaccaaaaa cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt
     3961 ttgccaatgt tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa
     4021 tacaacgatt ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt
     4081 gttcattcca ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata
     4141 tgatttgatc agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact
     4201 gccgatcaat cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca
     4261 aaatccaatc aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc
     4321 ttcctcattc atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa
     4381 tgcttccacc gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac
     4441 ctttccatca gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt
     4501 tcgataagtc gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg
     4561 actcggcagt ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag
     4621 tggtgaaaaa ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag
     4681 aaacaagctt ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac
     4741 tgttgtttcg tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa
     4801 ataataagga agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt
     4861 aattttttcg cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg
     4921 atgataatcc caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag
     4981 tttgttgaaa cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt
     5041 cgttttttca taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg
     5101 tgcttcgtca atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa
     5161 ataaacagtt ggatcaaata aataattgta atccccaatg attacgtcac accagatact
     5221 gacatctaat gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac
     5281 ttcacgagtg atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt
     5341 gtaataacca ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca
     5401 gatcttgtct ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc
     5461 tgtcatagca tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt
     5521 ttctccctct tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc
     5581 tgttccggta ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc
     5641 taccgctagt tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa
     5701 agaggcattg cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc
     5761 agtcagcttt ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt
     5821 gatcacttct tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg
     5881 ttcttgacta tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc
     5941 tggttctaaa tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc
     6001 gtctcttttg aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg
     6061 aactgttgtt tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt
     6121 gcggtggatt ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact
     6181 tccacgtcgc aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat
     6241 ttccaccacc tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc
     6301 tctttccaaa cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg
     6361 gatttttgga tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc
     6421 cttctcttta gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag
     6481 aaataagtac ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag
     6541 ttttggctat caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga
     6601 agaaaactgt tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga
     6661 tgcggaacga atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct
     6721 tacccatact cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat
     6781 cccagtttat gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc
     6841 tggactcggt agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt
     6901 tgaaatgaaa acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca
     6961 ttctatagga agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt
     7021 tagaggaagt atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag
     7081 tattcggacc cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga
     7141 tgcaacgacg attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt
     7201 aggaggagaa taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt
     7261 tcaatacaat tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag
     7321 aaggcattca ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta
     7381 gtgacagtgg tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa
     7441 aagaaattcc ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag
     7501 gtggatatga catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg
     7561 atgaaatgtt taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag
     7621 ataccgctgg gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg
     7681 aagatatcgc ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg
     7741 gtttgacgat cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc
     7801 ttttaaatgg cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca
     7861 tcaatgatac gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat
     7921 ggggatattt taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt
     7981 cttctttttt ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa
     8041 aaacagattg ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag
     8101 gaaggaattt atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg
     8161 aataacggaa tggctgccca tcagtagtac tggtcatttg atcctagcag atgaattcat
     8221 caaactggat gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc
     8281 gatcttggct gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc
     8341 aacggtagaa aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc
     8401 agccatcatc attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt
     8461 accagttgcc attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa
     8521 caaaaacaaa acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct
     8581 tgttgggtta ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat
     8641 cctaggatct atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt
     8701 agggattcct gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg
     8761 gaattcattc aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt
     8821 ggtttccgtc ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc
     8881 ttttggctgg tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat
     8941 gtagaaaaaa ccagaacact tctcttattt gagaaatgtt ctggtttttt attttcttta
     9001 ataataatca ttataaataa taaaaacaac atcaagctta atcaccaaat gataaccatt
     9061 atcgttctca tttagctgtt attattatca attacatttt tattatgcta tccttgtttt
     9121 gacaaggagg tctaataacg atgactaatc agaaaaaagc ttttgcagca acaatcggct
     9181 gccttttatt tatgatcttc ggttttatct tcgaccgatt ctctcttacc atcaccccac
     9241 ttttttatat cagtgccatt atcatcggtg gatataaaca aacatcggaa ggcttgaaag
     9301 aattgtggaa tgatcggaca ctgaatgtgg atttattgat ggctttagct gctattggtg
     9361 cctgtttgat cggtaattac tttgaaggag caatgttgac attcatcttt tgtttaagcg
     9421 gtgcattaga ggaatacaca actaataaaa gtcaaaaaga aattactgct ttgatgaatc
     9481 ttcaaccaca aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa gtagatgttt
     9541 ctcaactaaa ggtggataac ttggtatttg tcgctaaagg agcagctgtt ccgatagatg
     9601 gctttttaga aagcacacag gcaacgatcg atgagtctgc attaagcggt gaatctgtgc
     9661 ctgtagaaaa atatttaggc gatgagatgt atgcaggaac gctgaatcaa ggaaatccca
     9721 tcacgattcg agtaacaaaa acgagttccg aaactgtctt tgctaagatt atccaattag
     9781 tagaagaagc gcagaatacg ccaactcaaa cagcttcatt tatcgaacga atcgaaaata
     9841 attatgtaaa attgattttg cttgctgttc ctctaatgat ccttctcccc cactttttcc
     9901 taggttggag ttgggatgaa agcttttatc gaggaatggt attattagtt gtggcttctc
     9961 cttgtgcgct tgtcgcatct gctacaccag cttctctagc tgctctttca aattctgcaa
    10021 aaaatgggat tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga
    10081 ttgcctttga taaaacagga acgctcacta aaggaaaacc agttgtaact gacagctttt
    10141 tctttgatga aaaggatttg gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc
    10201 atccacttgc tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa gaaatccaat
    10261 acttaccaat agaagaaatt actggttttg gattacaaac tacctattta ggtgcgcaat
    10321 ggaaagttgg caagcacgca tatgatcctg agacgatgat aatcagcaaa gagattgcgg
    10381 aaatgatcga acgtttggaa aatcaaggca aaaccgtgat ttatctttct aaagatcagc
    10441 agttaattgc tgttttaggt ctgctagata ttccaaaagc aaatacccaa caagtaattt
    10501 cctactttaa atcccaaaac atccatacaa gtatgataac cggcgaccat tctggcacag
    10561 caaaggccat tgctgaacaa gtagggattg aacattacta tgcaagctgt actccggaag
    10621 aaaaaacaca actagtcaaa aaagagaatg aactttacca agtaaatgcg atggttggtg
    10681 atggagtcaa tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct atggggcaag
    10741 ggacagatgc cgctatggat atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat
    10801 ttgtctacag tcatcggcta tctttaaaaa tgcggcggat catcaaacaa aatattattt
    10861 tttcgattct tgttattctc ttgttgattg cttctaactt cctgcaattt ttaaatctac
    10921 ctcttggagt tgtaggacac gaaggaagca caatattggt tattctgaat ggcttgcgtt
    10981 tactaagacc tttacctgat ctttctgatt cttcaaaaaa atgtaagtct tgtccactat
    11041 acaaagcgcg gctgtcttct gtcaaataag caaagtgttt ttttatgatt ttgtttattt
    11101 tatgaaaaaa acagcttatg aataggtctt gaaggatgaa ttcctacttt tgtaactttc
    11161 taaaaacatg ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg aaagttggct
    11221 ttttttagga attattttac ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc
    11281 ggcagctttt gttttgatta tgaagctcat accagcctcc cgttcctttt tcccttggct
    11341 tcaaggcaaa ggaatcaata ttggggtgac gatcatcaca gcggctattc taatccctat
    11401 cgcaacagga gaaatcggtc tacgtgatct attagaagcc tttaaatccc ctattggctg
    11461 ggtagctgtt ttttgcggtg ggctagtggc tgtcttatct tccaagggtg taggattgat
    11521 tgcttctgat ccacaaatca ctgttgcact tgttttcggc acgattttag gtgttgtctt
    11581 cttaagagga atcgccgccg gaccggtcat tgcttctggt atcacttact gtatcttaca
    11641 agtatttgct ttaggaaaat agaacaaatt ttgtctattt taggagattc ttaacacccc
    11701 caatttactg atccgtgata tactgacaac gaggtgatac aatgttcgaa aaattaaagt
    11761 atttcatagg aacaatcggc tggtttcttc tagcggtact tttgattctg ggcttagctt
    11821 atctattatt tcctgatttg atacaaaatg tgtttgatac tgctttttga ttgacctatt
    11881 aaaacgatat aggggatgct cctgcgatgc aggggctttt tttaatcaat gttttgtaat
    11941 aatttaggcc gtgccaatga gacttcctat agaattcgat gctctttttt ctttttggct
    12001 cttctgctct tttttgttat actagattac agtggacaaa agaaagaatg atcgcatcca
    12061 cttcaacgaa tatcaaaatg gaagaattag tgcctgtttt caggttactt tgaaaagaaa
    12121 ggaagacgct tgtggaaact tttgaaggtc ttttaattga tacgtacgtt ttacaagaac
    12181 agccaatgat gctgactggg atcaacttgc agcttgagga ccgtgtgatc aatggcctgc
    12241 ttaaagatcg ctatttggca aaatatatct tacgtttgcc cctaaataaa tatgtgctgg
    12301 aagtaagtgg caaatggaac cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa
    12361 atatcgatga atacattaaa ataatcggta caccagaata gcctttactg atcaaaggat
    12421 actggttata aaataactgc agtttcttaa tagaaaaaca aagaacaaca gggaatcgta
    12481 tcccttttca aaaaaaactt tgcaaacggt tctctttgtt atataataac ttagtaccaa
    12541 acattcccat tcaaactttg tttaaataga taaataaaaa tctagtttgg tttgacgcta
    12601 ttgcctccca caatagcgtc tttttgattt ttttcagctt gtactatccg tttttactga
    12661 aaaaaaatcc taataaaaga ctgttaagga aaaaaacttg tcatgttcat attgcaaagc
    12721 acgctgcctt taggtataat aatgaatgtt gaattatgcg gtaacacgcg taagaactta
    12781 agttatcgga ggtgaaacta atgccaaaca ttgaatctgc aatcaaacgt gttcgtacaa
    12841 gcgaaaacgc taatgtgaaa aattcatctc aaacaagcgc tatgcgtact gcaatcaaaa
    12901 aatttgaaga tgctgtagct tcaggtgctg acaacgtaga tgcgttatat aaagaagctg
    12961 ttaaagctat tgatatggct gaatcaaaag gtctaatcca caaaaacaaa gctaaccgcg
    13021 acaaatctcg tttaagcaaa aaaatcgcta aataaaagaa tcggcttcgg ccgatttttt
    13081 tatttgtcaa aaaaaggaca aaaacaaacg accttcccgc atgctttgcc ctcgtctttc
    13141 ttcttatcga tttcttaaca actgccctgt gcgcaaaacg aatagttgaa aacttaattc
    13201 tttttcgatt tgtcccgttt tgatttgata atccatctca actaattgat caaacaactg
    13261 taccagcaaa tgttcatcga atttttttac ttcctgcatg gcaagtttta cacgatatgg
    13321 atggattttc aatgtttcag caatatttgc ctgctgataa ccaatcttta caagaaattt
    13381 tgtctgtaaa aataaacgta attgagaaag caaaatcgca ttaattttga ttgtttcttc
    13441 cccttgcata actaaatctt cgtataaacg caaagcttgt tccgtcttcc cttttaaaat
    13501 atattgggtc atgtcaaaaa tattatgttc taacgtttta ggaactaatt cttctacttc
    13561 tcttaccgtg atttgctgtt gattttgtcc ataaaggacc aatttctgta actctctcat
    13621 cattttggat aaatctaagt cggtcaacct caagaataaa tcaattgcac ttcgttccat
    13681 tttgattgag gaattctcta aggtattcat cagatattga cgtacttctt tttcattcaa
    13741 ctgttggaca ttgatgataa ttgctgtttt tttcaattgt ttcgttactt ttttccgttc
    13801 atccaacttt tcatatggcg caaagaatac taatacagtc gattctaatg gttctttcaa
    13861 ataagccgtc aataaatcag tattttgttc gattccattg tttacttttt cacctgttaa
    13921 aaaataagga ttctctacga atactaaccg ttgatctccg aaaaacggca acgtctctgc
    13981 ttcagctact acctcatcta aacttgcatt ttccatgtca aaacttaaaa aatttaagtc
    14041 atcttcgccc tctaattgga tttttttgac aatttctgtc cgaacttgat cttggagaaa
    14101 cttttctgtt cctaaaatca agtaacaaga tgcaagtttt tctttacgta ttttttgtaa
    14161 ctctgcttgt atactcaaat tactcaccac tttcaaaaat tatcctacca tgagcagatg
    14221 agaattacta gtctatcatt gtttccacta gcccatgagg atgaaaaact ggatgccagc
    14281 gataggtgat cattccttgt tgatccgttc gccatgtttt tatctgattt tcttctagtg
    14341 tctccaatac ttcgctgtgc gggtgaccat aacgattatt tacgccgcag gatataatcc
    14401 catgttttgc ttcaatatgg gaaagaaaat ctgtgttact tgacgtacgg ctaccgtgat
    14461 gacctaattt gactacatcg gctttcagat taggataaat caaaagcaat tgtttttcca
    14521 ttgcttgatt caaatctcct aaaaataaaa agcgcctatt gttatattcc atccatagac
    14581 caatcgaatc ttcatttctt ccctctccct tttcaaaagg agtcaataca taaagatcat
    14641 aataaccgct gatcctgtcc ccatgtttga tttcaatgat ctcagtacca tttttttgag
    14701 cttcagataa atgcctttgt aatggctcat ttcgtccagc gccccaaccg atataaattt
    14761 ttttgatagt aaaatgctga gataacgtgg gtaattctcc cacatgatcc gtatcgtcgt
    14821 gtgtcaagat gagtttatcg attacactta tcccctgacc ttttagatat ggaacgatat
    14881 ttctttcagc aggaggacgt atcatacctt gcgcccactt ttctctttca aagtgtaact
    14941 gacctccagt atcgatcaaa atcgtttctt ttcgaaaagg actttgcaaa aagatactgt
    15001 ctccttgacc gacatcgata aaagcgatcg ttgaagaaaa gcgtaaaaat ggcaaagtca
    15061 aaaaaataaa agaaaaaaga ccaacataaa acaagtgttt cacaggttgt tctttcaacc
    15121 tatccataca aatcagtaat ccgaaaatat acatcccaga tagcacaagg ggcaaacgtc
    15181 ctataatgag agacgaccct gaagaaaaat cgattattcg ctcaaataag attaatccct
    15241 cttctatcag caaaagtaaa gtttccggca gccagcgatt gaagagtgtg agaaataaca
    15301 caccaggaag aaaaagaaac ttgaataccg gtgcaatcag taatgtaaaa agactaccta
    15361 aaataggcca ctcataaaaa tgccatacaa tcaacggcat cgtgatcata gtaaacagga
    15421 cgtgagaggc cgtttttttc aaccaatctc catcaaaaga aatcaggata aacaaaaaac
    15481 tcatgaaaaa actaagctgt ccacccgcat gaaaaagaaa tcgaggatca aatagtaaaa
    15541 gaagccaaaa gacaactgca aataagtcca tggtggaata atgcttattc agcaatttga
    15601 ctagcaatcg caataaaaac agcaaactag cacgcaaaac agaaatagaa cttcctgcaa
    15661 tagaaacata acagatagtt agtagactca acggaataat actgacgtgt agtggccatc
    15721 cgatacgtcg aaataaataa tacagccacc ctaaataaaa ttggatgtgt agacccgata
    15781 gactgaacag atgcagcaaa ccactctccc taaaaatttc ttctggttct ctgaatcctg
    15841 catctttata gccccataat aaagaattaa tatatacact tgttttttgc ggaaattcag
    15901 attggacaaa ggagataaaa gatcctcttt ttctctgaag ccagtttcgc cagtgatttt
    15961 gcatggtgat ctgttcaatc ttttgaattt ggaaccgtcc agaaattcta tggttcctga
    16021 agtaagcttc ttggtcaaaa ttataaagat tccgcttttt tgctggagag atgaactcgc
    16081 cttcagcagt caattctatt ccctttcctc ggaagttaag ccatgttttc aattctgctt
    16141 ctgtgtttac ttgataagaa agctggagct tttgtttatt ctctgataac cttccttcca
    16201 ttgtcaaaaa attaccattt gcacgaatcg tatctgtaaa tacagtaatt tgcgcagatg
    16261 ttgttttttc ctctaaaaat gaatactctt gtagcctata agaaatggat accgttatgc
    16321 tacacaaaat acacaagtac gcgataaaac gtccttcttt tagcgaaaac cataaaaaaa
    16381 ttccggcaag aagcaaacga taaccccaat tgtcagtgta atacaacaaa cagataatca
    16441 tcgacgtaat tgccggaaaa atcaaccgat ttcgaacatg cctttctaaa acgctagaca
    16501 tgttttcctg ttaattcatt ttgaagtttt gaaaaatgct gtgagtctaa agcaacttgg
    16561 tgtacagata cacctaattg ttcgatcaac gcttgggcat aaggatcatt tcgatagtca
    16621 tggagataat tgatcccttt cacaccagct tgcaacaaag cctttgtaca ggccaaacat
    16681 ggaaaatgtg tgacatagat ttctgaacct tccgtcggga taccaagttt tgcacactgt
    16741 aggagtgcat tcatctctgc atggatcgtt cgcaagcagt gtccatccac gacgtaacac
    16801 ccttcatcta tgcagtggac atctccactg actgctccat tatagccgcc agcaatgatc
    16861 cttttttctc gaacgagtgt cgctcctact tctaaacgtg tacacgtact acgtaaagat
    16921 aacagtactg cctgagccat aaagtactgg tcccaaggaa tgcgttcatt atgtgactta
    16981 tcagtaactt tttctttatt cgtatcgcat gcttttgaag atttttctaa catgttttat
    17041 attcctccgt tttttcaagt tgattaaaaa gtactcattg cttaaacaaa atattcttta
    17101 aatataacga atcatcctgt ctgacaaaag atcctaccct tcactgccga aatgaagtcc
    17161 gtcatccatt agcccattat aaaaattctt cgaatccatt tgttcaagaa attccgtata
    17221 aaaatcgata cttcacaatc atggcgtttt gctacttctg tgagaacaga gatatattct
    17281 ttgatcaact tgttattccg atatttttgt tttgtctcat cgactgctgg tggcgtgata
    17341 aagaaagcat gatcagaact atagtctttt ttcagctgaa ctatcatcca ctgcagattt
    17401 tttggtacgt ttctttagaa agttgtttat gctctgctag atcattcgtc ccaatgagaa
    17461 taaatacttt atctgctgta tagctaagta catctttggc aaagcgttct cgtagagctt
    17521 gactgttatt gccgctgaca gccgtattca taatatgaat cccagatatt ttattttgga
    17581 gatagacatt cagcattggt ttttttaatg tttctttgcg aacaatcaag ctatttccta
    17641 tccaaaaata ctacctcctc gtcctgccaa gctcttagaa ttggattatt cttagtatcc
    17701 attagcgtcc atttattgct cgtgccagtc gattttgaat cattctattt aattgtaacg
    17761 aagtttttta gtttttcaac tgtcttttca ccgataccag aaatctcctg caggtcttcc
    17821 actttctgga aggatccgtt ttcttctcta aatcgaatga tttcttgcgc tttgactgat
    17881 ccgatcccag gaatctgctg aagttctgat ggagtggctg tattgatatt tattttggga
    17941 ggctcggaat ctgctgtagg ctgttttcct tccgaggatg ccaactgatc aagatgttct
    18001 ggcagctctt cacctacagc agggatatat aggacttgct gatcttctat cttcgcagaa
    18061 aagttgattc gatcgctatc cgcttcttct aataatccac ctgcacgctt tagtacatca
    18121 tagacccgtt cttctgaaga aaaggaatag ataccaggct ccttcacact tcctttgata
    18181 tcggtgtaca tgacctttgg acgagcatcc tcttctacat cgtttccttc agctgatgga
    18241 tcttctccac tggaactaaa cgaagaaagt atttcttttg cagcagtctc ttctgtttct
    18301 ctataaaata gcaaatttga taataaaaaa ccacttgcga tacaaaagat aagaactatc
    18361 atagacatcc aaatgatcaa tttgggtgaa gctagtattt tcttgctgat tttttctatc
    18421 attgttcttt tttcaaagaa tgaccagtaa aaatcattca ttactccttt catttaaaat
    18481 tgaagaaaca gtgagctctg gcaaaatcgt cccttcaaca cctaattacg tc
//
LOCUS       DACCPY010000059        18389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCPY010000059 DACCPY010000000
VERSION     DACCPY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.060"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IVO69_001766"
     CDS             <1..883
                     /locus_tag="IVO69_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7400541.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IVO69_001767"
     CDS             931..3060
                     /locus_tag="IVO69_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7400542.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IVO69_001768"
     CDS             3035..3724
                     /locus_tag="IVO69_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400543.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IVO69_001769"
     CDS             complement(4132..4818)
                     /locus_tag="IVO69_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7400544.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IVO69_001770"
     CDS             complement(4954..5148)
                     /locus_tag="IVO69_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400545.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IVO69_001771"
     CDS             complement(5198..5638)
                     /locus_tag="IVO69_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7400546.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IVO69_001772"
     CDS             complement(5642..6487)
                     /locus_tag="IVO69_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7400547.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IVO69_001773"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IVO69_001773"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7400548.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8130..9332)
                     /locus_tag="IVO69_001774"
     CDS             complement(8130..9332)
                     /locus_tag="IVO69_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400549.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9319..11106)
                     /locus_tag="IVO69_001775"
     CDS             complement(9319..11106)
                     /locus_tag="IVO69_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400550.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11119..12072)
                     /locus_tag="IVO69_001776"
     CDS             complement(11119..12072)
                     /locus_tag="IVO69_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400551.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12257..12607)
                     /locus_tag="IVO69_001777"
     CDS             complement(12257..12607)
                     /locus_tag="IVO69_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7400552.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12807..13886
                     /gene="pepA"
                     /locus_tag="IVO69_001778"
     CDS             12807..13886
                     /gene="pepA"
                     /locus_tag="IVO69_001778"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7400553.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14029..14349
                     /locus_tag="IVO69_001779"
     CDS             14029..14349
                     /locus_tag="IVO69_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7400554.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14371..14835
                     /locus_tag="IVO69_001780"
     CDS             14371..14835
                     /locus_tag="IVO69_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7400555.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15031..15636
                     /locus_tag="IVO69_001781"
     CDS             15031..15636
                     /locus_tag="IVO69_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7400556.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15675..16964)
                     /locus_tag="IVO69_001782"
     CDS             complement(15675..16964)
                     /locus_tag="IVO69_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7400557.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17392..17871
                     /gene="rlmH"
                     /locus_tag="IVO69_001783"
     CDS             17392..17871
                     /gene="rlmH"
                     /locus_tag="IVO69_001783"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7400558.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18074..18352
                     /locus_tag="IVO69_001784"
                     /pseudo
     CDS             18074..18352
                     /locus_tag="IVO69_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5977 a         3371 c         3643 g         5398 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagccgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtatttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattgtttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaactc cggctatctc tgacagctgg agtttttatg
     8101 tttaaaaatg aattagtaca tcggtattct taaaagtaat ttagttcgtc ttttgatttc
     8161 tcaggaggga tcaaaaaggc gaaagcacta agtatagcaa aacttgtgat gatttgcgta
     8221 cgatacgtaa accaataatg aatctgtgag tgattagcta aaatattgta ccaaatatac
     8281 ggcgttacag cagctaaaac aaacaaaact agaagttgga acggacgcca gctatttcgt
     8341 ttacgataag caaaatagag aaaaacaaga caagtaatca atagcaataa tattgctact
     8401 tttggataca tcaatctaat attgcttttc agcatctcta atcgatgcaa cgggtatttg
     8461 tcgtttcctt ccgttcgaaa gaggatttgg ttgattgcat cttttacaat gtttttgttc
     8521 aatatgatcg atgataggat ccatttagcc acccatgtca aaccatagcc taatccccaa
     8581 aagataccgg taaaaacaaa actgcctact tgtttgctca atgaactgcg tttatccatt
     8641 aaatacttat ttgttacata ataaataatg atcacaggta tcccccaaga gatgaccggt
     8701 gttgtcaaca agtcaaaaaa gttagctaaa gaaccaatga caaagaaata caagaataat
     8761 tgttggttgg tgttggagag ttgtggtttt gttaaaaata gtaatacaga caataaagtc
     8821 aaaatccaaa tatttgaaaa ttgcatactg aagaaaaagg ttgcagcatt tcctatagca
     8881 agcgaaatca acaaggctgt cgttaaatag atgtcgctac acttggttaa aaagtagctc
     8941 gtcaatccaa tcagcagaaa aagaatggct gcgtaaatca ttcgaatact ccccaaactc
     9001 atccctagca gtaaaggtcg taaaaaaagc tgataaccat gccaataacg gggataatcc
     9061 gccatagaca tcgcgttctc caaggagttc aattcaggat ctgcttttgt ttttcgtatc
     9121 atcaaatgat cagtaaaatt atctaaccga gtgcctagca tatttccttg attgatagaa
     9181 ggatacattc cttctttttc aaccatctgt agagattttt ctgcattcga ttcaatagag
     9241 tgctgcggaa tgaaaaaagt aattgtcagt agtacgcaat agctagcgat caagagaaca
     9301 aaagcaaaaa atatcttttt aaatagtttc atgagtatct cctatatctg ttttcatttt
     9361 ttctctttta tctagtaaaa aaggagagag gatataaaca ggtagaccta atgctaaaac
     9421 aaacacatac cgtaaactaa aagccacagg agtagcaatc ataatagtag caaaaacaag
     9481 cataccggga agaaaaagga gtaaataccg atattttttc ttaaagattg ctaaaagtgc
     9541 actgccgata gctaaccaca gtaatgtgcc ggaaccaaag aaatcacgag catcgatgat
     9601 ttttttcatt cctgacaatc cagtaagatg ttcaatcaaa tctacttgct tcataccata
     9661 attattcgtg tttacacgag tatctaaaaa gccgtaacta ttttttgtac caggtaccca
     9721 gaacccgaac gtttctaaaa tataagcatc tgtatagatt tttatattac ttggtaaatt
     9781 tcttgcccat agtgatagaa attctttttt atgtgtattc aaaaactcat tgttaaaatt
     9841 tttatgccac ttgattgagt ctgcaagact aggagaatac gcttcataat cttttatcgg
     9901 gagtagctga ttcatgaatg ctttatcttt cttagacaag tcaccatcca taacaatagt
     9961 tctagaaatt tgctgcaaag gaacacctaa ttgttcagca gtttctgtag gaatagcata
    10021 acgagccatc aacgggtgga tgagtagtcc agcaatcact gtataaccca gcaccacttg
    10081 aaagaatgct tgataccttt tttgtttcaa aataatcaag ccgcaaatca aacctgtgcc
    10141 ggttactaca taaatcccat tatttcttag ataggtggtc agtaaagcaa ggatgattac
    10201 gtaaatctga ttgagaatcg ttggcttttc tcgttgaaaa ataaagtcaa aaaccgctaa
    10261 ggaaaataaa aagcaagcga tagagaacaa agtatctttt tgcatgttca aggcgtagat
    10321 tggaaaaacc ggtgcaaatg caaaatagca taaactaatg agtaccggaa aaaaatggat
    10381 gtttcttttt aacagccagc aaagagaata acttatcgct gaagctgtta tcaatgtttg
    10441 aaaaatcgaa aataaaaata caccgtgatt gatcgtatca gttggtaaat catgaacaaa
    10501 tagcttgacc agtaaggtga ataagactgg atgatgattg cttataggaa catcacccat
    10561 agattgggcg accgaactta acgaatcagg caaaaccact ccaggataca atgccaacag
    10621 ataaggaagc caacacagaa ctaaaaagac agtaaagaat aaaaaatatt ttcttgaaga
    10681 gaaagcctgt gtgttttgag cagtaaaaaa tgtttccagt tttggcattg ataaataaat
    10741 cgattgtaaa aataaaaaag aatacacaaa aatggagaaa taaacaagaa agtaaaaaat
    10801 acttccatca gcaatcgttg tctttgtata gtttgctgag acatactgaa attgtatctg
    10861 tctgccaata gaaagtgtaa gagcaaaagc tgcagaaaaa aacaatcggg caatagctgc
    10921 aactttgttt tttattgaaa acaactctat tccatattgc ttaaaagcaa gtagcaaaac
    10981 catggctaaa ccaatgatta ctgttggatt caaagttcct agaccataag aagtaaaaaa
    11041 agcaaaacct gacgcaaata aagttgcaaa aaaagtagga aatagattct cctttttctt
    11101 atgcataaaa aaggcctcct acttcatttc tttattttga tattcgtggt agacatggta
    11161 taaattcaat tcatattctt tcttggcatt tgtgacgacc gtatccaaaa tcaaccctgt
    11221 tactaatgaa agaagggcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg
    11281 tgtaataaag cttgtcagca tatattcacg aatcacagga atccccatga ccaatccaaa
    11341 taaaaagaag aagatggtcc agatcccaaa gaaaagcaaa ggtttataat ctttgcacat
    11401 tttgatgatc atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct
    11461 accttcagga cgatcgcgat aatcgattgg aatctcaacg aacttgaatt ttttatctaa
    11521 agcatgaatc gtcaattctg tttcgatttg aaaaccagaa ctcataatag ggaagctttt
    11581 gacaaaaatt cgattgaatc ctcgatagcc ggtcatgaca tcgcgaattt ttgttttata
    11641 taagtaagta attgtatttt taaccagatt attcccaaaa tcatggaaag gacgcttatt
    11701 ttcatcaaaa taggttccat ttgatagacg atcgccaatg accatatctg cttcgccaga
    11761 acgtaatttc tcaagcaaac catgtgcagc ttctgcagga taagtatcat caccatctac
    11821 catcaaataa taatcggcat caatatcaaa aaacatttga cggattacat tcccttttcc
    11881 ttgacgtggt tctttcttta cgattgctcc gccttgaacg gccaattcgt atgttcgatc
    11941 ggtcgaatta ttatcataga cataaatatc tgcttctggt aattcccttt taaaatcttc
    12001 aataacagtt gaaattgtcg cctcttcgtt ataacaagga atgagcacgg caattttttc
    12061 acgtgattcc atttgtcact ctccttatat aataaaacta atcttatttc aataataccg
    12121 tataataact acaaatacta gctattccaa gaggaaatag ccctattttt ataaaaatca
    12181 cccactataa aagaaggata ggatacctct cttttagata gtgggtgcgt attcgttatt
    12241 tcattttttt gacgtattaa tctattgcgc ccttttttct tgaaatgtct aatacggttc
    12301 ctgtatgagc atctgctaaa aattcataat aaacagtttc gccatcttct atccgggaaa
    12361 gcccaccccg ataagcactc gaccgaacgg cgaatttccg aataggttct ttttcaaaag
    12421 agatccaagc accttcgatt gcgccttctt ttaaaaaaga tgttttgata ttttcaagca
    12481 cttggtctgc tgataacact tttttgcgat gataccaaat cgttgaagct accccactca
    12541 ataatcctag accgatccca acggctaacc cgcctttaaa atagcttaat tcgctttctt
    12601 catacatgtg gatatacctc cttcttctca cttgctactc tttgtgttta ttttaccaca
    12661 ctctcatatt ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac
    12721 gatcggtctg cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt
    12781 tagtctgaaa tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt
    12841 taacagaatt acaaggaaca agcggttttg aacaagacgt tcgcgcctat atggaaagtc
    12901 atatggaacc gctggtagat gagttacagt tagatggttt gggcggcatt tttgggctgc
    12961 gccaccatga agaagcggat gcacctcgtg taatggtcgc agcacatatg gatgaagtcg
    13021 gattcatgct gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggat
    13081 ggaaccctta cgttgtttct gctcaacgtt ttacacttaa aacctctact ggtaagaatt
    13141 atccatgtat ttcttcttcc gttcccccac atttattacg aggaacaagc ggacaaaaat
    13201 cagtagaagt aacagatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga
    13261 gttacggtgt tcttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca
    13321 acgggaagaa tatcatcagc aaatcgtggg acaaccgcta tggctgtaca atggtattag
    13381 aagctttaga agcgttacaa aatgaaacat taggtcatac attgattgca ggcgcgaatg
    13441 tccaagaaga agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc
    13501 tgttctttgc agtagactgc tctgcagcag atgacacagt gacgaaaaat ggcacattcg
    13561 gacatttagg agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc
    13621 gtttgcgtga gtacttgcta gatatcgctg aaacaaacaa catcccttat caatactttg
    13681 tttcaaaagg cggtacagat gcaggtgctg ctcatacaca aaatgaagga atcccaagta
    13741 cagtgattgg cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg
    13801 attttgaagc tgcacgagaa atgttgattc aaacattgaa aggtttagat aaatcaacag
    13861 tgaacacgat cgtatacggg aaataacgcg atagtgaaac tataaaatct ggcaaaagca
    13921 accttttgtc agattttttt tataaaaaga ggatcaatgt ttgttagttc gaaagaaagg
    13981 tgtataattt aattgaaaaa tacataaaca gaaagtagag gaaaaagaat gattatccca
    14041 aatagtttag aagaattagc aggctacgta gaaactggaa aaagtgtatt tttctttact
    14101 gccggctggt gcggagactg ccgttttatc aaaccgcaaa tgcctgaaat cgaaaatgac
    14161 ttttctgatt ggcaatttat tgaagtagac cgtgacaagt acatagatgt agccgctgaa
    14221 tggaacattt ttggtatccc aagttttgtc gtgatccaag acggaaaaga attaggtcgt
    14281 ctagtgaata aagatcgtaa aacaaaacag gaaatcgaat cgttcctaga aagtgtaata
    14341 aaaggctgac atctgttagg aggggtcaag atgaatcaag aatataagaa cattttagta
    14401 ggtattgacg gtagtgaaca agcacttgct gctttcaaaa aagcgttaga agtggctcgc
    14461 cgtaataatg ggaaagtata tgtagcaaac gtaatcgatc agcaatttta taattttatg
    14521 ggctatgctc ctattgatca aaatctagtc gatcagcaaa ctgaaacagc caagcaaatg
    14581 atcgaagaat gtaaagaata tggaaaatct gttgattaca caaatataga aggaattatt
    14641 gcatacggct ctgttcgtga atccatggca gtcaaattgc caaaaaaata tgcaatcgat
    14701 ctaatcatgg ttgggcagtc tggactaaat gcagtcgaac gctttatgac aggaagtgta
    14761 gccagctacg tcatacgtca ttcaccttgt gatgtcttga tcgtttctga cgaagaagaa
    14821 cagccaagta attaagcaag tggaacttgt ataacctttt taatcaaaca gtaaagggac
    14881 tgtgacaaaa gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaagctt
    14941 cttcggaaat aaagcatgaa aaaaatctca tgcagtgaat gcaatttttt gttaagataa
    15001 atctcagcaa aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc
    15061 ggagacactt tgatggttat catagccgac gataaaggaa cagaaaacac tgtcgaacgt
    15121 aaatggaatg ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc
    15181 catatctccg atcatctaac aattgaaggg aacggccaag taagcttaac agatgaagaa
    15241 ttagcgaaat tgaatcgatt gattaaagaa gcaggattcg aagaacagct tgaagctgat
    15301 catgaaccga agatcgttgt cgggtatgta aaaacatgcg tcccgcatcc tgactctgac
    15361 catttatcga ttactgagac agaagtggac aatgggacag tattgcaaat tgtttgcggt
    15421 gcaccaaata tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc catgatgcca
    15481 gatggaatga tgatctggcc aggaactttg cgaggggtag ccagctatgg aatgatttgt
    15541 tctgctcgtg aattgcattt gccaaatgct ccagaaaaaa aaggaatctt agtattgcct
    15601 gaggatgccg tagtcggaga agtatttcca aaataaagag gttgtgacaa gtctttgtca
    15661 caacctcttt tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc
    15721 gtcaattcaa cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa
    15781 tctcccactt ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg
    15841 tcatcgatct tagtaatgac gtcgtattgt ttcaacccag ctttttctgc cggtgtagct
    15901 gtttggacat ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt
    15961 tcttgttgct gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt
    16021 gtcacttttc cgtctttttc cagttggttg atgatattca ctacatcgtt acttgggatg
    16081 gcaaatccca ttccttctac gcttacatta gaagtcgatt cagaagtact tgcgatctta
    16141 cttgagttga taccgataac ttgtccttcg atattcacta aaggaccacc agaatttccc
    16201 gggttgatcg ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc
    16261 gtacttgtta cttgtcggtt caaggaagaa atgattcctg aagtgacaga attcgcatat
    16321 tcagatccta atggggaacc aatcgcaatc gcaggttcac caaccttcaa agcacttgag
    16381 tccccaaaag aggcaaccgt ttcgactttg tctgaattga tcttcaacac cgctaaatct
    16441 gaataggcat ctgttccaac taattcagct tttaccttcg taccgtcttt catcatgact
    16501 tccaaacctt gctgtccatc tactacgtga ttgtttgtga ctacataagc agtattcccg
    16561 tcttttttgt agattacgcc gcttccttca ctataagctt ccaaattact atcatctgag
    16621 ctgctacttt cttcttgctg cccgaataat ccgccaaaac cgcttgattg gttttgactt
    16681 tgtaagttga taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca
    16741 gaatctacat tgactttgac attttccaca actgtttcac cggcagaatt ttgattgccg
    16801 cttgtagcag tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc
    16861 agtagtcctc caacgatccc tccaacaagc cccagaccca acttacgcca gaggctatta
    16921 tttttatttt ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat
    16981 tacattcatt tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac
    17041 tttgaacttt ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat
    17101 tttctttttc cacagatatt gtgtaaaact acgcggatag gattggaaaa ataagggata
    17161 aaacatatcc acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta
    17221 atgaatgtga tgaaataagg taaaacaacg ctgttttctg tggataaacc tgtggatgtt
    17281 gtgcataact gttggggaaa tctttttaac aaaaatagtc aaaataaaca tatagacgaa
    17341 aaaattgatt ataataaaca aagtgagcgt gaagatcaaa ggagagaaaa catgaatatc
    17401 aaaattattt cagtaggaaa attaaaagaa aaatacttga tccaaggtat caatgagtat
    17461 gtcaaacggc tgaatgccta tgcgaaaatc gaattgatcg aagttcctga cgaaaaagcc
    17521 ccggagaatt taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg agaacggatc
    17581 ttagcgaaaa tcaaagaaca agaatacgtc tatggcttag caattgaagg gaaaaatccc
    17641 acaagcgaat cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt
    17701 gtttttgtta tcggcggttc tttaggactc agcgaagccg tcatgaaaag aagcaatgct
    17761 cagatctctt ttggcaaaat gacgtatcca catcaattga tgcgcctgat cctcgtcgag
    17821 caaatctacc gtgccttccg gatcaacgcg ggggctccat accacaaata atgatatttt
    17881 ttaagagaaa aaattatccc tattttaaat aatagcaata ggtagtggta gggagaaatt
    17941 tataaataac ttttttattt gctttggtag ccaagtaagt tgcaaaagaa aaatagatac
    18001 tgttaaaaag ataatgtaga catagaaaca aatacgtaaa attttagtta gaataatggc
    18061 aaggagaaaa aacatgatag atgaaaaaaa gtgtttaaaa aatctattgg aggatatgga
    18121 tgatattgaa gaaaaactat ctaaatggac taggaaaaca aatatatttc agatacttaa
    18181 gctatctaaa aatgaaataa gacatagtaa ttttttagct tttttgtttg ctccaaatga
    18241 aacccacaat ttatctgatg agttttttaa aatgtttttg aaaaggtata tagattctaa
    18301 tgatgatacc aaagttgcta tcaattattt tgatgcttta cttaatagct acccgtggtg
    18361 ctagttaatt ttaatatcct aaagaatat
//
LOCUS       DACCPY010000060        18105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCPY010000060 DACCPY010000000
VERSION     DACCPY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.061"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(11..1519)
                     /locus_tag="IVO69_001785"
     CDS             complement(11..1519)
                     /locus_tag="IVO69_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7400559.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1678..1869
                     /locus_tag="IVO69_001786"
     CDS             1678..1869
                     /locus_tag="IVO69_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400560.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2072..2989
                     /locus_tag="IVO69_001787"
     CDS             2072..2989
                     /locus_tag="IVO69_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7400561.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2982..4055
                     /locus_tag="IVO69_001788"
     CDS             2982..4055
                     /locus_tag="IVO69_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400562.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4203..4721
                     /locus_tag="IVO69_001789"
     CDS             4203..4721
                     /locus_tag="IVO69_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400563.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4702..5094
                     /locus_tag="IVO69_001790"
     CDS             4702..5094
                     /locus_tag="IVO69_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7400564.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5170..6105
                     /locus_tag="IVO69_001791"
     CDS             5170..6105
                     /locus_tag="IVO69_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7400565.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6238..7017
                     /locus_tag="IVO69_001792"
     CDS             6238..7017
                     /locus_tag="IVO69_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7400566.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7010..7327
                     /locus_tag="IVO69_001793"
     CDS             7010..7327
                     /locus_tag="IVO69_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7400567.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7571..9181
                     /locus_tag="IVO69_001794"
     CDS             7571..9181
                     /locus_tag="IVO69_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7400568.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9338..10186
                     /locus_tag="IVO69_001795"
     CDS             9338..10186
                     /locus_tag="IVO69_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7400569.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10417..10896
                     /locus_tag="IVO69_001796"
     CDS             10417..10896
                     /locus_tag="IVO69_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400570.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10929..13157
                     /locus_tag="IVO69_001797"
     CDS             10929..13157
                     /locus_tag="IVO69_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7400571.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13286..14929
                     /locus_tag="IVO69_001798"
     CDS             13286..14929
                     /locus_tag="IVO69_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7400572.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15002..15547)
                     /locus_tag="IVO69_001799"
     CDS             complement(15002..15547)
                     /locus_tag="IVO69_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7400573.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15570..16055)
                     /locus_tag="IVO69_001800"
     CDS             complement(15570..16055)
                     /locus_tag="IVO69_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7400574.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16045..16857)
                     /locus_tag="IVO69_001801"
     CDS             complement(16045..16857)
                     /locus_tag="IVO69_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7400575.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(16996..>18105)
                     /locus_tag="IVO69_001802"
     CDS             complement(16996..>18105)
                     /locus_tag="IVO69_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7400576.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5879 a         3299 c         3626 g         5301 t
ORIGIN      
        1 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
       61 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
      121 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
      181 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
      241 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
      301 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
      361 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
      421 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
      481 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
      541 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
      601 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
      661 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
      721 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
      781 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
      841 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
      901 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
      961 aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
     1021 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
     1081 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
     1141 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
     1201 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
     1261 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
     1321 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
     1381 gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt
     1441 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
     1501 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
     1561 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
     1621 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
     1681 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
     1741 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
     1801 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
     1861 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
     1921 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
     1981 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
     2041 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
     2101 tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc
     2161 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
     2221 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
     2281 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
     2341 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
     2401 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
     2461 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
     2521 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
     2581 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
     2641 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
     2701 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
     2761 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
     2821 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
     2881 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
     2941 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
     3001 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
     3061 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
     3121 aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
     3181 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
     3241 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
     3301 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
     3361 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
     3421 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
     3481 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
     3541 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
     3601 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
     3661 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
     3721 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
     3781 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
     3841 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
     3901 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
     3961 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
     4021 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
     4081 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
     4141 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
     4201 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
     4261 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
     4321 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
     4381 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
     4441 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
     4501 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
     4561 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
     4621 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
     4681 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
     4741 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
     4801 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
     4861 tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
     4921 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
     4981 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
     5041 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
     5101 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     5161 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     5221 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     5281 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     5341 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     5401 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     5461 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     5521 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     5581 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     5641 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     5701 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     5761 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     5821 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     5881 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     5941 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     6001 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     6061 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     6121 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     6181 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     6241 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     6301 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     6361 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     6421 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     6481 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     6541 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     6601 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     6661 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     6721 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     6781 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     6841 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     6901 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     6961 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     7021 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     7081 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     7141 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     7201 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     7261 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     7321 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     7381 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     7441 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     7501 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     7561 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     7621 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     7681 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     7741 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     7801 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     7861 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     7921 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     7981 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     8041 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     8101 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     8161 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     8221 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     8281 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     8341 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     8401 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     8461 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     8521 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     8581 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     8641 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     8701 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     8761 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     8821 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     8881 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     8941 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     9001 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     9061 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     9121 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     9181 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     9241 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     9301 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     9361 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     9421 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     9481 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     9541 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     9601 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     9661 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     9721 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     9781 cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga acaatttagt
     9841 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     9901 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     9961 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    10021 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    10081 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    10141 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    10201 aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt
    10261 gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct
    10321 cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact
    10381 tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa
    10441 cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat
    10501 acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat
    10561 atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta
    10621 ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa
    10681 gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga
    10741 ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac
    10801 atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt
    10861 attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga
    10921 agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc
    10981 aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct
    11041 ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag
    11101 gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc
    11161 cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat
    11221 tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa
    11281 cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt
    11341 ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa
    11401 tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag
    11461 agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc
    11521 tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg
    11581 tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa
    11641 gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac
    11701 gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta
    11761 tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt
    11821 cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc
    11881 caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt
    11941 tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc
    12001 atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa
    12061 acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg
    12121 aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc
    12181 ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa
    12241 caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca
    12301 agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag
    12361 gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca
    12421 tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg
    12481 aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag
    12541 tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt
    12601 tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag
    12661 cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt
    12721 tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata
    12781 aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc
    12841 aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg
    12901 caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata
    12961 caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa
    13021 tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg
    13081 aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga
    13141 ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct
    13201 tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga
    13261 gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt
    13321 aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat
    13381 caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat
    13441 caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga
    13501 tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga
    13561 attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg
    13621 gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga
    13681 cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga
    13741 gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt
    13801 aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga
    13861 taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc
    13921 agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca
    13981 tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag
    14041 tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat
    14101 attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct
    14161 taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc
    14221 tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc
    14281 cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac
    14341 gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga
    14401 tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac
    14461 aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat
    14521 gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa
    14581 tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca
    14641 attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta
    14701 taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga
    14761 acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga
    14821 agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa
    14881 aggcatacta gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt
    14941 taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat
    15001 ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt
    15061 gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca
    15121 taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga
    15181 caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag
    15241 gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg
    15301 ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact
    15361 cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc
    15421 ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg
    15481 cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca
    15541 acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga
    15601 agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct
    15661 ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg
    15721 atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg
    15781 ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac
    15841 tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca
    15901 cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat
    15961 ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc
    16021 agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc
    16081 gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc
    16141 aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc
    16201 tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt
    16261 atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt catctccagc
    16321 catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc
    16381 gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc
    16441 tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat
    16501 atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat
    16561 tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt
    16621 caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg
    16681 tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc
    16741 tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt
    16801 ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac
    16861 attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc
    16921 atattttcat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt
    16981 tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg
    17041 cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa
    17101 gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc
    17161 taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa
    17221 aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat
    17281 caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc
    17341 tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata
    17401 agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac
    17461 attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc
    17521 tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca
    17581 ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa
    17641 tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga
    17701 aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt
    17761 ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt
    17821 tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct
    17881 ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac
    17941 tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg
    18001 gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga
    18061 ttgagaaaca gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACCPY010000061        18057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCPY010000061 DACCPY010000000
VERSION     DACCPY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.062"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IVO69_001803"
                     /pseudo
     CDS             316..675
                     /locus_tag="IVO69_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IVO69_001804"
     CDS             complement(970..2103)
                     /locus_tag="IVO69_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7400577.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IVO69_001805"
     CDS             complement(2094..2762)
                     /locus_tag="IVO69_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400578.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IVO69_001806"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IVO69_001806"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7400579.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IVO69_001807"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IVO69_001807"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7400580.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IVO69_001808"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IVO69_001808"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7400581.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IVO69_001809"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IVO69_001809"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7400582.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IVO69_001810"
     CDS             complement(6138..6911)
                     /locus_tag="IVO69_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7400583.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IVO69_001811"
     CDS             7076..7555
                     /locus_tag="IVO69_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400584.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IVO69_001812"
     CDS             7612..7914
                     /locus_tag="IVO69_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7400585.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IVO69_001813"
     CDS             7936..9393
                     /locus_tag="IVO69_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP7400586.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IVO69_001814"
     CDS             complement(9986..10738)
                     /locus_tag="IVO69_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7400587.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IVO69_001815"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IVO69_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..12157)
                     /locus_tag="IVO69_001816"
                     /pseudo
     CDS             complement(11199..12157)
                     /locus_tag="IVO69_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12363..14045)
                     /locus_tag="IVO69_001817"
     CDS             complement(12363..14045)
                     /locus_tag="IVO69_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400588.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(14056..14379)
                     /locus_tag="IVO69_001818"
     CDS             complement(14056..14379)
                     /locus_tag="IVO69_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7400589.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(14402..15808)
                     /gene="lacG"
                     /locus_tag="IVO69_001819"
     CDS             complement(14402..15808)
                     /gene="lacG"
                     /locus_tag="IVO69_001819"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7400590.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            16275..17282
                     /locus_tag="IVO69_001820"
     CDS             16275..17282
                     /locus_tag="IVO69_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400591.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17676..>18057)
                     /locus_tag="IVO69_001821"
     CDS             complement(17676..>18057)
                     /locus_tag="IVO69_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP7400592.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5431 a         3811 c         2979 g         5836 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct
    11401 ctttgcgaag gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata
    11461 gatcgccagt tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta
    11521 ggcatggatt tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc
    11581 ggtttcaaag taataataac cttggataaa cactcaacca atgtaataac agcgctcttg
    11641 tgtttaactc ccacaatggt gtcgccttcg agatgaccaa attctttagt caggtgtgga
    11701 tagagactgt tccgttcatg aatcgttctg cgaaaggcct gttttccacg cttttcttga
    11761 tgattattcg gtttacgttt ccccttcata ggtaacgact gtgtgtcaaa aaggcctttt
    11821 ttaaacatcc gatagagtgt acgaacagaa caatcaattg gaaactcgcc acggccgata
    11881 atcacatcgg gtgaccaccc ttgagctacc ttttcttgaa catatgttga ttgttcatca
    11941 gataaaacgg ttgaacgtcg tccacaacga gatttatttt gtttgtattg acggtaataa
    12001 tctagcactt ttttccttgt ttgagaaacg aataaacatt ataaatggtt tgtctagagc
    12061 gatttaaact ttgtgcgaca agagcgacag gtttctcgat attgaaataa gactctatga
    12121 ttactagttc gtctgttgta agatgggtgt aggtcatttg tactcactcc ttattaattc
    12181 tttggtcgga actattttga gtgtagcaca aatgatcttt ttagttgtct agcttaattt
    12241 tacaatcgac gcctcaaaaa gaatacccga aatttaaaca gcaaaaaaag atagcgctta
    12301 ccattgtgtt taagataaaa cgctagctaa gttggctagc gctttattaa tctaagcaag
    12361 ctctaaaatt gcttcaatac aaaatcaact gctcctttag gatcacgtgt taaattgata
    12421 tattctgcac ctttagttgc aattactttt atccctaatt tgtctgcatc tttacggaga
    12481 tcatccaaat aggaattcac ttgaggtgct agaacgatta aatcaaaatt tttcatcata
    12541 tcataatgag aaccataagc tcctgcttta gcagaaagat taacacctgt ctcttttgcg
    12601 ccctcttcta atgcattagc caacattgca ctcgttccgg caccagcaca aagtactaaa
    12661 acgcgctttc cttcaccaac cgatgctttt gcttcatcaa ctataactgc cgcagttgca
    12721 ccaacttctt ggatttcttc gaccaattct tcttttactt cttctgcagc aatgtttttt
    12781 aactcttgtt ctactaattc agaatcatag gccttaacaa aaggtaaata aataatcgca
    12841 tcgacaacaa tcaatactac cgctaataca atcgccaaca cactaaaccc ggttcctaaa
    12901 accaagccaa tcggtgcggg cgtcgcccaa ggcaatacgg atataaagct attcatcccc
    12961 aagacatcga caaaaagttt gaatatccat acgttagcga ttggtgctaa taaaaatgga
    13021 attagaaagt acggattcaa aataatagga gtagcaaata gtaacggttc atttaccgca
    13081 aacgaaactg gaataaatga agctttacca actgctttca attgttttga tttagcgaac
    13141 aacatgaaca gaaaaggaac aactagtgtc gctccagtac cacccatcgt tccaacaaag
    13201 tttcctagac caactgttaa tacttttgag gcttgtgcac cgtttttaaa taattcaaag
    13261 ttagtttcta cgttcgcata aataattgca gcaatcgctg gttcaacaat cgatggtcca
    13321 tgaacaccaa caaaccagaa cattgccatg gctccccaaa tgattgcaat accgacatat
    13381 ccatcagcag cactaaataa tggctgcaat aattgaatta cacctgctgc aaatgatgtt
    13441 ccaaaaaatt gacgaactcc cgtatcaatc aaaacggcaa taaatactgc aattgaaaag
    13501 gggataatat ctctaaacat ctgtgtgatt gttccgggaa cttcttttgg catacggatc
    13561 gtcaaatctc ttttaacaca gaatttatac acatttacag taacaaacgc agaaacgaaa
    13621 gctgctaata atccctttgt ccctaaataa tcagtagcta tcccaccatc aatttgatca
    13681 gctgccatca ttacaaaacc acaaattgac gctaacataa cagaaacttc attaatctgc
    13741 ttacctgaat cagataattg acggttcatt gaattagcta aacatcgagc tgttgtagca
    13801 acaaccaaaa gaccaactaa tcccattgaa taaccataaa ctttccaaag ccacgtcgag
    13861 aatgcctctg gtaacgtcac gttaaatact tctggaacag ccgcaatcat aataaaaata
    13921 cttgaaaata gaatagctgg catagcagtt aaaaagccat cgcgaattgc ccctaaataa
    13981 atattacgtg agattttttc aaaaaacggt tttcccttct caatttgctt aataatcgta
    14041 ttcatttttc ttctcctatg ctttagcaaa tttttctttg tacaattcaa caaaatcatt
    14101 gataagttct cttaatagaa tcgttgtcat taaatggtct tgcccatgaa taaaaattac
    14161 gcctacttcc atttcgttcc ctgacgcttc ttcagctaaa catttcgttt gtgagttatg
    14221 cgctaaggtt aaattttcat cagcatccgc taataatcgt tcaacattat caaaattacc
    14281 ttctctcact tccttcaaca ctgttaataa tgaacttctg gcatctcctg cataagcaac
    14341 aatttcaaat cctaataatt gcaattcttt cctatccatt tccttgccct cctaactatt
    14401 tttatattat cgtttttgtt tcgctaacaa atttatacca atatgcagac tctttcggat
    14461 atcttttttg tgtatcgaaa tctacataga atagtccata acgcttagtg tatccattaa
    14521 cccaagagaa aacatccatt aatgaccaaa tgaaataccc ctttacatta actcccattt
    14581 tgatagcatc ggaaatcgca ttaaaatgct ggcgtaaata atcgattcga tcatcatcta
    14641 gaataatccc atcttcaaaa ttatccttat accctagccc gttttcagta atgtatattt
    14701 tattataatt tggatattcg tcttggattc gtacaagtaa atcatataac ccttttggat
    14761 aaatgatcca atcccaatcg gttttcggaa gatcctcacg cacgattctc tcgccaatcc
    14821 ctttaatgca atagacttgg ctaccttttt ctccggtagt gttgtagtga agtgtgcttt
    14881 ctcctttgta ctctttaacc caatggctac catagttatt gataccaaga aaatcatttc
    14941 gatttgctgc ttttttcatc tctctaaaat cattagaatc aatatctgca gattgcccat
    15001 ttgctgctaa aatctcatta atatgtgcca ttgtttcatc tgagtaatac cctaaataag
    15061 tagcatctaa taagaactta attgataacg catcatctaa tttcgcagca tgcttatctt
    15121 cttctttgtc acttgctgga tattttgtct ctagcgagtg aacaacacca atttcccctt
    15181 tatatccacc atctttaaaa atgtttactg tctttgcgtg cgcatacatc atgttgtgta
    15241 aacaagttac agtcttctct atatcaaatt gaacttctgg aggaaacgat ccgatcaaat
    15301 attgatttga agcaactgga taaatctcat taaatgtact ccaataatga acctcagaaa
    15361 attcatcgaa acagaactgt gcatagttta cgaaatgttc aacattttct cgatttaaaa
    15421 aatctccatt atcaaatagt tctttagggg tatcaaaatg atgaagagtt acaaaaggaa
    15481 taataccatg cttattcaat tcagcaaata ccttatgata atattccact cctgtttggt
    15541 tcacttcccc atagcctttt ggaaaaatac ggctccatgc aatagacatc ctaatactat
    15601 caattttaaa ttttttgcat aattcagcat caactggata ttgattataa aaatcactag
    15661 ctggatcagc agtatagcgt cccttttcta caaggaaagt atcccacgct acttgccctt
    15721 ttccaccttc ttttgtagag ccttctgctt gataagctgc cgtagctcct cccataataa
    15781 agtcttctgg taaagtttta taattcattt taatctctcc ttgaaaatta gtttgatcaa
    15841 tatgccaata atccataaat cacctcagca taattttaac atgtataagt aaattttttt
    15901 acacaacgaa aaaccgacct cgaatatcta ttttattgcg atatacaagc acttatatcc
    15961 cttgatgtaa aataaatata ccattcaaaa accgttttgt aaacgttttt ttgtaaaata
    16021 aatttatctt tttactaatt taatttacac gaaacaagaa aacggactga ttcccactaa
    16081 aatcaatccc acctactaat cattctccaa gtggctatcc attatcgatt caatcttctc
    16141 tgttaatgga ttaggctctc tgctgatatc agagtttcgt ttcctgctag aaaaagtaat
    16201 ttaagttact aaaagagcag tttcgattta ctttaacaaa gaaaaggatt attatatccc
    16261 taggaggatg aacaatggtt actattagag atattgcaaa aaaatctggt tactcaacgg
    16321 ctactatatc aagacttttt aaaggtgatg agtcactctc tattacatct gatactaaaa
    16381 acaaaataat cgttacagcc aaatctttag gatatgatct gacaaaaatt aagactactc
    16441 tgtacaagat tgccattctt ttttggatct cagaggaaca gttaaaaaag gatgtctact
    16501 atcaacatgt caaagatgat ttgaaaaatt atagtaaaaa agctaatcta gatttgattt
    16561 tcatctctaa aaacgatggt gtaaaaagca ttcctgacga tattactgga tttatcgcaa
    16621 taggacaatt gacaaaaaag gaattgtact acttaaatca aaaagggtat aagggggtaa
    16681 tccgaggaat aaacccatta cctgaacact tcgatactgt agaacctgat ttccacgaaa
    16741 tggccagaca agcaattaac tactttatta atgaaggttt cacaaaaatt ggatttattg
    16801 gaggaaattt ctttaaccta gaaacgggta aagaagagat tgatagccgt gaatatgcct
    16861 tccgatatca tttagaaaag agcggactac tcaatgaaaa gtatattttc tcccaaggat
    16921 catttactgt tgacgaaggg tatcaattaa ctcttaaaat gaaagctgag ttaaatgaag
    16981 atatgcccga ggcatgtttc ataccatctg acactatagc tgccggcgtt cttcagggct
    17041 gccatgaatt gggtattaac atccctaaag atatggcagt aataagtatt aatgatgacg
    17101 acatcgcaaa attcgttgca ccaccactta caacttttaa gatagatatc gaagagatag
    17161 ctaaaacctg tgtagattta ttaagtgatc aactggtata ttcgcgggac atatcaaaga
    17221 aaacattaat cggcgcaaaa ttgaccgtta ggaaaagttt tatccctaaa gaagaggtgt
    17281 aaaataagta attgaaactt atctttaaaa ctataattgt gtccacatag tagccgagca
    17341 atcgggttta tgcctattat gttttccata aataatttga tggtgatatt ttgacgtaaa
    17401 tacaatatga ttttttcatc gccaaattgt gcgcgctaat ttttgtttat cattcagaac
    17461 atttcctcct ttttatctaa gtatgaggtt ggataaacgc ttcatcttag tatcttgggg
    17521 gattttttat ctcgttggga atatatcaag catcagagcg cgcaaaaaga actggtcgaa
    17581 aaatcaatct accagttcaa tctcattcac tgtgatacta tgttttatac agataacgcc
    17641 atattataaa acaagttgta ttccgcaaat gggttctatt caactcttaa actatctata
    17701 aaagattcag ccaactccat tagcctctcg taatttagtt ggtgaacagc acttttttca
    17761 aaaaattttg aaccaattcc tacataagag gctccattgt tcaaatagtt ttgtgcatta
    17821 gtctgatcta cccctcctac agccatcaaa ggtaaacttc ctaaaggggc ttgcacttga
    17881 ttgaaatatt ccggtccaag acagttggcg gggaacacct taacaatatc agcacccatc
    17941 tcaaacattt tgaatatctc tgtgggcgac atggccccag gaattgttag aaggttctgt
    18001 tgcgaagttt gaaaatcaaa cgcgccctct ctgaactcca aatatcttag gctgtta
//
LOCUS       DACCPY010000062        18013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCPY010000062 DACCPY010000000
VERSION     DACCPY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.063"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(7..1047)
                     /locus_tag="IVO69_001822"
     CDS             complement(7..1047)
                     /locus_tag="IVO69_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7400593.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1324..3126)
                     /locus_tag="IVO69_001823"
     CDS             complement(1324..3126)
                     /locus_tag="IVO69_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400594.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3140..3892)
                     /locus_tag="IVO69_001824"
                     /pseudo
     CDS             complement(3140..3892)
                     /locus_tag="IVO69_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3901..4263)
                     /locus_tag="IVO69_001825"
     CDS             complement(3901..4263)
                     /locus_tag="IVO69_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7400595.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4439..5311)
                     /locus_tag="IVO69_001826"
     CDS             complement(4439..5311)
                     /locus_tag="IVO69_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7400596.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5337..5849)
                     /locus_tag="IVO69_001827"
     CDS             complement(5337..5849)
                     /locus_tag="IVO69_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7400597.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(5987..6817)
                     /locus_tag="IVO69_001828"
     CDS             complement(5987..6817)
                     /locus_tag="IVO69_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7400598.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(6833..7441)
                     /locus_tag="IVO69_001829"
     CDS             complement(6833..7441)
                     /locus_tag="IVO69_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7400599.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7434..7751)
                     /locus_tag="IVO69_001830"
     CDS             complement(7434..7751)
                     /locus_tag="IVO69_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7400600.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7907..8635)
                     /locus_tag="IVO69_001831"
     CDS             complement(7907..8635)
                     /locus_tag="IVO69_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7400601.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8794..9675
                     /locus_tag="IVO69_001832"
     CDS             8794..9675
                     /locus_tag="IVO69_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7400602.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9912..10955)
                     /locus_tag="IVO69_001833"
     CDS             complement(9912..10955)
                     /locus_tag="IVO69_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7400603.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10958..11626)
                     /locus_tag="IVO69_001834"
     CDS             complement(10958..11626)
                     /locus_tag="IVO69_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400604.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11768..14104
                     /locus_tag="IVO69_001835"
     CDS             11768..14104
                     /locus_tag="IVO69_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7400605.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14219..14530)
                     /locus_tag="IVO69_001836"
     CDS             complement(14219..14530)
                     /locus_tag="IVO69_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400606.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14711..16186
                     /locus_tag="IVO69_001837"
     CDS             14711..16186
                     /locus_tag="IVO69_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7400607.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16233..16817
                     /locus_tag="IVO69_001838"
     CDS             16233..16817
                     /locus_tag="IVO69_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7400608.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16819..17238
                     /locus_tag="IVO69_001839"
     CDS             16819..17238
                     /locus_tag="IVO69_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7400609.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17519..17803)
                     /locus_tag="IVO69_001840"
     CDS             complement(17519..17803)
                     /locus_tag="IVO69_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7400610.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT         5512 a         3720 c         3133 g         5648 t
ORIGIN      
        1 tttattctat ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg
       61 ttccagagtg acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc
      121 aaatgtgata ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc
      181 aaaatttgct gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt
      241 ttcatctggg cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa
      301 tggatgatcg ataccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc
      361 taatttttta ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt
      421 aggcgttgta tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc
      481 gtcgattgac tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa
      541 tactgttttg cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag
      601 cgtataccgt acttctacat ctaaagttcc aggaaatccg ttttccatgt ctgggctagt
      661 ctgatagaaa atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt
      721 ggcttcaaag ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac
      781 ttcgaacgtt tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc
      841 gattgaagca ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa
      901 gatcaactct cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag
      961 atcactgaca gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc
     1021 gccaaaattt tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact
     1081 gattccagta tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa
     1141 atttactata ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa
     1201 cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
     1261 acgaaaaata ggaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct
     1321 ttttcacaat ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta
     1381 gatgaaatac aatacgaaga agatcgtaaa cggaacccaa atatctttgt atggattttg
     1441 catgaataga ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg
     1501 tgctaagaat aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt
     1561 tcctatcttt tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt
     1621 gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa
     1681 ttcaaaacca gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta
     1741 ctctactttt tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg
     1801 tttcaggttt gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt
     1861 cagttgatca aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa
     1921 ttcatagctt cgaagttcct cttgtacagt cagttcattc attttttcag caggaacttt
     1981 ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc
     2041 cgcaaattca cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc
     2101 agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat
     2161 cactagatca tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa
     2221 tcctaccgtc aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg
     2281 tgtatattca cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa
     2341 tgaaatctgt tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat
     2401 gaaataagtt cctaatacga tcgtaactaa ggctactcct ataccaaaga cttgataggt
     2461 ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc
     2521 aaggaaaaac aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa
     2581 tgctaatatc ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa
     2641 aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga
     2701 aatattgaga tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc
     2761 gacagctgaa cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat
     2821 ctgtgcgatt tttctgcctt ttgctcctag catcataaac attgcatagt ctttttgccg
     2881 catcgtcatc aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa
     2941 aacagaaccg aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa
     3001 agcttcgttg cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc
     3061 tgagaataac accaggtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca
     3121 aagcatagtc aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat
     3181 agaaggaatc acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga
     3241 tgaataaaat acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg
     3301 atacatgatc ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg
     3361 aatccagcgc acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac
     3421 gtgcggcggc gacccgttgt ttttgtccac cagaaatctc agaaggatag ctttccaaaa
     3481 tatgagagat ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc
     3541 tgacgccttg taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata
     3601 aattgaagtc ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc
     3661 cttttaatgt ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta
     3721 atgtggataa aatattcaaa agtgtcgatt taccagaacc agaagcgccc atgatcccaa
     3781 taaattcgcc ttattctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat
     3841 tgttttttcc gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc
     3901 ctacccttta attagttttt ccagtgctgt tttcggaacg tctttttttt cgacttcttc
     3961 atacgaagtg acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag
     4021 cggtcgagtc aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc
     4081 aggcaggata tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt
     4141 tgtaccatct gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc
     4201 gattttcacc cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt
     4261 cactttttcc ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg
     4321 ccttcgaatc aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa
     4381 aagagttcaa gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc
     4441 acttacggtc tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga
     4501 ttgagacaag taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt
     4561 gtccatcttc gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg
     4621 tttgtcgagc agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt
     4681 ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta
     4741 aacgctcttg cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg
     4801 tcaaatcatt ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg
     4861 tctcactttg gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg
     4921 tatcaacgac tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat
     4981 tgacttttaa aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg
     5041 ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa
     5101 acaaaattac ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg
     5161 tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacatgccta
     5221 aatagaagaa aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac
     5281 cattgatata aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa
     5341 taaatatcta atgttacttt tttaattttt tttcgaatgc gcgccattac accttctagt
     5401 tctaactctt tgtcttcagt gatgtaccaa ccgcttgcct gtgtccctcc ggctgcctgc
     5461 ggtaaataat ccaattttgt tcgaacccag tgcttttctt ctgtaaaata gtcttctacc
     5521 aagacctcta gatccatccc tgctgaaaga ggcaaatctt cccgaacaga tttaaagata
     5581 aatcgttcct tcttcccatc atacttcaat attcctgtat cgataatcgg taaagaataa
     5641 taatccagct ctttcttcac cgtcaataaa tgtttgaaaa cttcatggta cacttgctct
     5701 aattggcgac cttctgaatt gttcgggtaa ttaaccatca ctttttcttt gtctggatca
     5761 tagaaggttt ggcttagttg agtgatataa gtatgtaatc tttccatttc tgtctttaac
     5821 ttttctcttt gaccttcttc taccttcata tactctttca ccctgttttc tttttagata
     5881 ttttctttct tattttacta tagttatgtg cgttaagcga tgaaaaacag caaagattgg
     5941 aaggatcttt gctgtttttt cttaaaatat ccagttgagt acaggatcaa tctgtttcat
     6001 tgtcctcttg atccgtcgta atattgatcg atgtgtcttt agcagtgatc tttagctttt
     6061 tctgtccttg ttctaacaaa gtatttgtta ttggctggta gtctttttca atataagatt
     6121 caccagacgt ttggatagta tagccactgt tttccatctc atgcaaagat aaattaccac
     6181 cgtttaggat gacttttcct tctttgctga tcaaactgtc ttctagatgg aagttgctgt
     6241 tttcacctgt gacgatcaag ttatccacat tggcctcatc tagtcgaagt gagcctaatt
     6301 catctttcgc agttattgtt cctcctgtaa attcattgaa cgatacccag ccatcatgtg
     6361 tcttagtatt cacttttttg attgatactt cgttgatatc aattgcacta ttattccctt
     6421 cgattgtcaa ttcagataat tcatgatctg cagggacaac aatagtgatt tgtgcacttg
     6481 tgtcttctaa actaatgatt gcacctgttg attttttgac tgcattcaca gtcaacacgc
     6541 catttttctc ctttatttct ggcttttctg tcttatcata agtcgttttt atctcaaaat
     6601 ccaatccgcg tatgaccgaa actgtttgat tttctgcatg gatgacgatt ttgtttgcat
     6661 ccttgatttt ttttgtctca gtaaccatat cgatcaaccg aggaccatta tcccaagtca
     6721 gactcttttg agcgcctaat gtataagttg cacctgctag gaggccacca gtaagcatca
     6781 gaccaatacc ggtaatcaaa aaatgtttca atctcaattt atgtgtcctc cttcaaacgc
     6841 ccgctgaatg cagatctctt tttgtaataa aacgtcgtcc catccattta acaaagttca
     6901 tcatgacata aaaaagccct tttgttcctt tcagcaacag tggcgaaata aatactgcgc
     6961 ttcctgtggc gaacagaccg acaccgataa agaataatcc actcagaatc gattgtccaa
     7021 acacactgat tcctgagatg atcatgaata ggccaacacc taagatactg atacataaaa
     7081 catagatact aaaaatgagc gctagtaaag caaccaatag tcccccaata accaatataa
     7141 accccagtgc tagtggaatg aggacaggag aagcaaataa accgataatg attaaccaaa
     7201 tcaggcgaat ttgtcttttt ggatttttca ctaactcgtt ttctgcttgt tcttcttctg
     7261 aaaagtaact gatttttaat tttaaggcaa actgctttgg tgtaccatat tcatcaatga
     7321 tctgatctaa tgtttttcca gaatctaaaa aatattcctc ataatactgg atcaaatcaa
     7381 aacgatcgtc ttcaggaatt tcttgaagat gatccacaac ttcattcaaa tattcattca
     7441 tttgtgctct cctccaataa ccggtctaaa gaatctcgaa aaacaatcca ttcatcaata
     7501 atttcttcta aatgacgact tcccattggt gtcaacgagt aatatcgacg gtttctgcct
     7561 tcaaaaggtt catcataggt ctcaagatat ccttcttttt tcaaccttct caaaaccgga
     7621 tatagtgtag attcactgac agagagactt tcttgtacga tttttgttaa ggcataccca
     7681 tacatatctt ccttgcttaa tgctgctaac acagtaccat caagaagaac agttggaatc
     7741 tggatgtgca ttacgtcacc ttctattctc aagtgtaata ttttatttca atctatatta
     7801 tacaacatat aatatagttt caaaatcagt ttgtcaaata caaaaaaact gactaaaaga
     7861 tttgatcctt tttagtcagc aaaaaatggc ttctctttga tttttattat aacagtggca
     7921 taatccaatt tgtccacatc cagctcagtg gcatgataac gatcattgca ggtatcatgt
     7981 cagctactgg gaacattttc acgttgatca ttctaaaacc ggtagcgacc atcaagaatc
     8041 cgccgcaagc tttaaaatct aagatcatgt ctggtgtagt caatggataa ataaattttg
     8101 ccaataagaa caaaatataa aaaataatga attggggaat tgctatcaca gaaacgacat
     8161 accctaaatt acacgcaaaa atagctgctg tgaaaaaatc aaggacagat ttagaaatca
     8221 aaatcgtact gtctcctgtc atcccagaat ctaaacttcc atagatcccc gtaccacttg
     8281 cacaaaatag aacaatgacc gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa
     8341 gcttttcact agggaagatt ttagaaatag gtacttgcat cagcgcagcc cccttattta
     8401 tcccattacc taaatgaaca atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa
     8461 cagccggcat atttttcatc ggcgcaatgg aatagattcc catccccatc gaacacacac
     8521 caaaaaccat cgtcagctgt gctttaaaat gttcacttaa tttatttcct actaatccgc
     8581 caattagtcc gccaaacaaa acggaaccta cattgatcaa aactccagtt ggcatcaaaa
     8641 aactcctctc tttttctacg aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt
     8701 cgctgcaaga aagattgtaa cagacagaga agattacacc aattcatttt ttgtgttaaa
     8761 gtattcttta ttggaataag taggaggaat atgatggata tccataaatt cgaagttttt
     8821 cttgatttag ccgaaacaat gaatttcacc aaaactgctg atcgacaatt caccacccaa
     8881 ggaaatattt ctaaacagat tctttcgtta gaaacagaat tagacgtcaa attattcgaa
     8941 cgcgcccata gaaaaatcga attgaccgaa gctggaagct tattgctccc atatgtaaaa
     9001 aatgtcgtag aacaatacca tgctatgcaa gaagtgcttg aaacctatac gaaagacaac
     9061 aatctttcat taaatatctt aacgataccg acaatgatca attataaagg tttttccaaa
     9121 atcaccgagt ttttgaaacg ccatcctgaa tttacggtcc agttgaaaga agtggaaagt
     9181 gtcgaattaa cttacaatga cgccaatgaa catagtgaca cgatccactt tgctagaagt
     9241 ttccaggccc caccaagttc gattgagtgg ttgcctactg aaacagacga ttttgtagca
     9301 gttctaccta aaaatcatcc tttagctagt aaaaagaaat tggatctaag cgaactgaaa
     9361 aaggagaact ttttgctcct tggccccact acgaacttgt atcaacctgt actagatctg
     9421 tgtcgcgaag ctggatttga accaaaaatc tcttataaag gagcaagaat cgacttgatc
     9481 atcaatatga ttgcaaatga catggggatt gctattgtta tggaaaaaac agtcaagaat
     9541 ctattaaaag aaaatactgt gattctccct atttctccaa caacagagag ttatcttgca
     9601 tttactcgta aagtcggtga gcattctttg gcttcagata cattttggga gtatttaaat
     9661 caagaagaaa actaagccct acattttctg tccatctatc cacccaaaaa aagagagcga
     9721 cgcatgactt tcgctctctt tcttgcatca tgtccttgtt tcaatgacac ctctaacatt
     9781 tctcgtagtt caataagaaa gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc
     9841 ctaatcacaa aaaaacgact aaaaacccct aaaaaccttc aatagaattt ttaggggttt
     9901 tattcttttt cctattgttt acttaaccgt atcattgctg tcgttcctac tccttcgata
     9961 ctgctataat caatacgtcc atcatgcagt tctagaattt ttttaaccag tgaaagtcct
    10021 agcccattcc cagaaatttg acgacttgta tctccttgat aaaatttttc aaacattcga
    10081 tctctggtct cttcattcat tcctacacct gaatcggtca cttccagtac aatctctgtg
    10141 gctgtctcaa atagtttgat atgaatctgt ccattgattt gattgtactt tatggcgtta
    10201 tccatgatat tcagccacac ttggtacaaa aactcttcat tgcctgtgta atttactgca
    10261 gcaagttcga tatccaattc tagctgttct ttctcccatt taggctgaaa aaaaacgatt
    10321 acttctctga tttgttcatc taatcgatac tctttctttt ccagttggat acgctggttt
    10381 tccagtttgt tcaattttaa gatgttctct gttaattgtg aaagctgcgt gatactttcg
    10441 ataatccgat ataaaaatat ttggcgttct tcgtcagaga gatttggggc ttgtaacaat
    10501 tgaacatatc cttggattgt tgctagtggt gttttgaatt catgggatac actggacaca
    10561 aagtcattcc tcagtgtctc gatactattg agttcttgga ccataacgtt gaaatcttta
    10621 tacagttgct gtacttctgc tactttctgc tgttcatcca ttcgaatcga aaaatctccc
    10681 gttgctacca gactcatatt tgtccctagc gcactgattg gatgcaggat acgccgccca
    10741 acaaaaatcg atattcctgt acccacgacc atactgaacc ccgcaaagac gaataacggg
    10801 tataacgttg gttttccagc cgattcagca ggcgagatag aatgtcgaga caataagaaa
    10861 ataaaaccga gaaaacacag cagtgtcact aagatcgaaa tgaagacaat actagcaaaa
    10921 tagatccata attgtgaaaa tagctttttt ttcatcttta aataaccgcc ttatagccta
    10981 gtccccgtac tgtgatgatt tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt
    11041 tgatatgtac atcgatcgtt cgttcttctg tattcgtatc taatccccaa atatcatcca
    11101 tcaactgttg gcgagtgaag atcttgttgg gataagacag taatttatac agaagcagaa
    11161 attctttttg aggcagaatc aacgaatgct gatcttgtaa cactgtataa gcatctagct
    11221 ctaattttgt ttgtccgatt atcaagctac tgctgtaatt gatctttgaa cgtcgaagca
    11281 atgcttccac acgtaggatc atctcattga catcgatggg ttttgtcata taatcatctg
    11341 ctcctaattt gaacccgcgt ttcttatcct caaaatcggc ctttgccgta atcattagaa
    11401 caggtgtgtc aaatctggtg ctacgtaatg agtctagcaa gtcatagcca tccattttcg
    11461 gcatcatgat atctgtaatg attaattcta tacgtgtcgc ttctagtttt ttcagcgcat
    11521 cctctccatt caatgcggtg atgactgaaa agcccgctcg ttccaacaca gaacggtaaa
    11581 gcaactgtaa ttgcggatca tcttctacta ctaaaatacg attcattgat ctccctctgt
    11641 tttttgaaaa atttctagat tttttcttgt agttcatctt cagttaacat ttatttcgta
    11701 tactacgatc attgttctga tatacttatt agtataccga atatttcaat acgggagaag
    11761 ggtgattatg cttcaattaa aagatataaa aaaatattat aaagtcggcg aaaccaccac
    11821 caaagcattg gatggtgtgt ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg
    11881 tcctagtggt tctggtaaaa cgactatgct gaatgtgatt ggtggactag ataattatga
    11941 ttccggtgat atggtaatca acggaaagtc tactaaagac tttaaggaca gtgactggga
    12001 tgcctaccga aacaactcta tcggtttcat ttttcaaagt tacaacttga ttggtcatct
    12061 aggcattatt gaaaacgttg aacttgggat gacattaagt ggtgtttcta aagacgaaaa
    12121 acgaaaacgg gcagaagatg cattgcaccg agtcggcttg acaaatcata tgcataaaaa
    12181 gccaaatcag ttatctggtg gacaaatgca gcgagtggcc atcgctcgag cactagccaa
    12241 tgacccagat atccttttgt gcgatgaacc aaccggagcg ttagatacag aaacaagtat
    12301 ccagatcatg gaattgatcc aagagttatc gaaagaaaaa ttagtgatca tggtcacaca
    12361 taatcctgaa ctggcccatc aatacgcaga tcgaatcatt gaattttctg atggtaagat
    12421 cttaacagat tcccatcctc acatcgaacg accaaaagat gatcaattta acttacgcag
    12481 aacaaagatg agtttttgga cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa
    12541 aggacgtact ttcttgacct cctttgcttc cagtattggg atcatcggaa tcgcgatcgt
    12601 tctgtccctt tcttctggat tccaaaaaca aatcgataac acgcaagctg aaacgatggc
    12661 gaaatttccg attactattt ctaaggtaac gactaaccag actcgtgacg atgccggatt
    12721 gggtgcaagc aaagcggatt atccagacag caaaacgatt actgcaaaag tcagcgaaga
    12781 agataaagca cagcacacga ataagattga tcaaacatac gtcgattacg taacagacat
    12841 cgatcctaac ttaagtaata atatcggatt tacccgtact acaggaatca atcttttacg
    12901 tgatgtaaat ggcaaagtac agcctgtcag cttctcgaat caaaatcctg atacggaatc
    12961 tctttcttta tctagtacga tgtcagcgat gacaggtgtg ggagtgtcaa gtttccctac
    13021 acagcttgac actagcaaag aaaacttttt aaaagacaat tattctttac ttgccggaag
    13081 ttatccagcc tcagcaacag atgtcgtgct gatcgtagac ggcaataata atactaatat
    13141 caacgcattg aagaatcttg gatttgatgt gaaagaagac gaaaaacttg attttgatga
    13201 tatcgttggt acaacattca aattagtaaa caacaatact tactatacaa aacttccaac
    13261 tgggaatttt atccctaata cagattatga tgcgatgtat cagaatgctt ctgatgaatt
    13321 gaagatctcg gggatcctac gcgtgaaatc ttcttctaca atgaatctct tatctccagg
    13381 aatcgcttat agcgatcaat taacgactca aatcgtcaac gaaaacaaag aatcagaaat
    13441 cgtcaaagct caaagagaca gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa
    13501 tgccaaacaa acattgctta gttatttagg tggagacagc ttgccttcaa gtatcatgat
    13561 ttatccgaac aattttgaag acaaagaaaa gatcttggat tatctagatg attacaataa
    13621 aggcaaatca gatgaagata aaatcattta tactgattta gctggaacga tgactgaact
    13681 gactggcgga ttgatggatg ctatcactta tgtccttatt gcttttgcag ggatctcttt
    13741 agtaacaagt atgatcatga tcagtatcat tacttataca tctgtgattg aacggacaaa
    13801 agaaatcggt gtactaaaag ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga
    13861 cgcagaaacg tgtattttag gtatttcttc tgggatatta ggtgtgttta ttgcatggct
    13921 agctacgttc cctatcaata gcatcttata caacatgacg gatctaaaaa atgttgctca
    13981 actaaatcct gtccatgcga ttattctagt gatcgtctct actatattga cgatgcttgg
    14041 cggtcatctt cctgcacgaa tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga
    14101 ataaggttgt gacaaccaca tttagatttg agtcataaga gcgctgaaat agtagaaaac
    14161 agcaagaaac gaggaagtaa tatgttcctt gtttcgcgct gtttttttta attatttttt
    14221 ataattctgg tatacttttc tttcctttgt accacaactc ttctggcgta tacctgtctt
    14281 ttccatttat gaatactgtt ccatctgctt caatcgttac ttcagtccca atccacatat
    14341 tacgagaaag tgcgagaaga ttttttcttt ttcctcttcc tttttttcca aattgcaagg
    14401 catcggttat ctcttctgtc caatgagatt tcacgtattt tacttctccg ttttcaaatg
    14461 taactttcaa aatattcgct atattttcca cttttactgc tttaggaaat gactcctcca
    14521 tttttttcac tttccttcct ccaacactaa gtatgcgctt tcttttagtg ataagtatat
    14581 catagtttat tctattatca aaaaaacgca tccattccat tgtagaatac tttaccgaac
    14641 aagttgaatt gttttcctct ttttcacctt ttactataga taggtaaaga caactaagga
    14701 ggaatctctc atgacagatc aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac
    14761 acttccaggt caatatcacg aaacagatat tgaaatcgat cctgaagcag aattatctgg
    14821 tgtttaccgt tatatcggtg ctggcggtac agtaaaacgt ccgactcaag aaggaccagc
    14881 tatgactttc aacaatatca aaggtttccc cggcacacgt gtgaacatcg gaacaatggc
    14941 tagtcgtaaa cgtgtaggtg aaattttgca ccatgattat cgaacacttg gtcgtttctt
    15001 gaaagacgct gtagaaaatc caatcaaacc tgtgaaagta gacaaagcag acgctccagc
    15061 acaagaagtc gttcatctag ctaccgatgc agacttcgat attcgcaaca ttttgcctgc
    15121 acctacaaat actcctgaag atgctggtcc atacatcaca atgggtgtag tcttaggatc
    15181 agatccagaa aaaacaatga gcgatgtaac gatccatcgt atggttctag aagataaaga
    15241 tacgatcggt atgtacatca tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt
    15301 cgaagccttg aataaaccaa tgcctatcac tatcaatatt ggtttagatc cagcaattgc
    15361 cattggcacg actttcgaac cacctaccac tccacttggc tacaatgaac tatggtgtgc
    15421 aggagctttg cgtaacgaac ctattcaaat cgttgatggt atagcagtcg atgaagtggg
    15481 tattgctcgt gctgaattca tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga
    15541 agatatcaac acgaactcag gaaaagcaat gccagaattt cctggctata ctggggatgc
    15601 taatccagct gtacaagtag tgaaagtcaa agcagttact caccgaaaag acaatccaat
    15661 catgcaaaca acgattggtc ccagtgaaga acacgtatct atggccggta ttcctacaga
    15721 agcaagtatt cttgccttgg tcgataaagc tattccagga aaagtagtaa atgtctacaa
    15781 tcctcctgca ggtggtggta aattgatgac catcatgcaa atccgtaaag aaaacgaagc
    15841 ggatgaaggt attcagcgtc aagcagctat tcttgcttta tcagccttca aagaattgaa
    15901 gacagtcatt ttagtcgatg aagatgttga tattttcgac atgaacgatg tagtttggac
    15961 tttcaacact cgtttccaag gagataaaga tatcatcgtg atcccaggaa tgaggaacca
    16021 tccattggac ccatcagaat taccagaata taatcctggc tctattcgtt tccgtgggat
    16081 aagtgcaaaa actatctttg acggtacagt tcctttcgat atgaaagaac attttgtacg
    16141 tgcaaaattc caagaagtag aagattggga aaaatatttg aaataatgaa tgatctgcaa
    16201 ctgttttgac atcataaaaa ggagtgcgga aaatggaaca attggcacaa cggaagaaaa
    16261 aaatcgtgat tggtgtaagt ggcgcttcag gcaccattta tgccatcaac ctagtgaaga
    16321 aactaaaaga atatccgatg atagaaacac acggaatcat cagtccatgg gcaaaacaga
    16381 acttaaagct ggaatccgac ttatcactaa ctgaattcga aagcctgttt gattaccatt
    16441 attcaaacaa agatttaggt gctgctgtcg ccagcggatc atttttgacc gatggaatga
    16501 tcatcgtacc ggcaagtatg aaaacagtcg caggtatctc tgttggttta ggcgacaacc
    16561 tgattgcacg tgctgctgat gtaacaatga aggagcaacg aaagttagtc attgttcctc
    16621 gtgaaacgcc attaaatgcc attcacttgg aaaacttgac taaattagca cgactaggtg
    16681 tgcaaatcat accaccaatt cctgcttttt ataatcagcc tcaaacgatc caagatcttg
    16741 ttaatcacca tacaatgaaa cttttggatc aatttgaaat tgtaaatgag cattcaaaac
    16801 gttggcaggg tgattaatat ggaaaaagaa acgatagttg aagtaaccaa actaggtttc
    16861 acgatccaag ctgctgttaa agtaatcggt aaagacttat tgatcacttt aacaggcgga
    16921 gatacaccac atatcggaac agtcactaca ttttctcgtg atacagagat gcaaactgta
    16981 cgtttcccta gccacgatgg tcggttccat aaagacgatg tcttagccga aaaaatagct
    17041 gtgatcatca aaactgccgt tccgggaagt tgtacgatca cttccggtat ccatgttgac
    17101 catatctcca atgcacaaat caacgcctct tttccgatgt cagaagagtt aggttacaag
    17161 attttagatt ggttggaaaa aatagatttc catatcaaag aaccagttta ttataaagat
    17221 ggtgaaaaac cattatgaaa gaagaggttg tgaaatcagc gttatgacct gagtatgaga
    17281 gaaaaaaatg gaaaaatagc ttctcatatt ttgccgtttt tgagctaata ccgagggaca
    17341 gctgattgaa cgccgtttat caagaggttg tgataaagcc gcttcgtttc gagtagtaag
    17401 aaaaattttg taaacatggg tttccacatt tttgcataaa tttgacttac taccgagaaa
    17461 ctggcttttg aacaccgttt atcaagaggt tgtgacacgt cttttgtcac aacctctttt
    17521 atttttgctt cactgtaact gctacaatat caccgatttc ttttccgatg tttttacgaa
    17581 tatcttttcg tagtccaagg atgtagcaga ctgaaccatc tggatttttc acgcccatat
    17641 tcacgatact gccttcatac ggcacattgt caaaagttgc ttggacttta actcttccag
    17701 caccgaattc ttttcttata tcatacggaa aaataatata tgccccgccc ttgccgactt
    17761 cggaagcata gatttttgat tcaaaagaat aaatttccct cattttattt ctcctatttt
    17821 ttacatctag tatagcatag caaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    17881 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    17941 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    18001 gctcacaacc tct
//
LOCUS       DACCPY010000063        17272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCPY010000063 DACCPY010000000
VERSION     DACCPY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.064"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(176..1462)
                     /locus_tag="IVO69_001841"
     CDS             complement(176..1462)
                     /locus_tag="IVO69_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7400611.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IVO69_001842"
     CDS             complement(1455..2723)
                     /locus_tag="IVO69_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7400612.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IVO69_001843"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IVO69_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7400613.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IVO69_001844"
     CDS             complement(3495..3941)
                     /locus_tag="IVO69_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400614.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IVO69_001845"
     CDS             complement(4034..4279)
                     /locus_tag="IVO69_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400615.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IVO69_001846"
     CDS             complement(4394..4972)
                     /locus_tag="IVO69_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7400616.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IVO69_001847"
     CDS             complement(5049..5579)
                     /locus_tag="IVO69_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7400617.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IVO69_001848"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IVO69_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7400618.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IVO69_001849"
     CDS             complement(6436..6831)
                     /locus_tag="IVO69_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7400619.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IVO69_001850"
     CDS             complement(6828..8240)
                     /locus_tag="IVO69_001850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7400620.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IVO69_001851"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IVO69_001851"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7400621.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IVO69_001852"
     CDS             complement(9095..10555)
                     /locus_tag="IVO69_001852"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7400622.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IVO69_001853"
     CDS             complement(10725..11882)
                     /locus_tag="IVO69_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7400623.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IVO69_001854"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IVO69_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7400624.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IVO69_001855"
     CDS             complement(13349..13876)
                     /locus_tag="IVO69_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7400625.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="IVO69_001856"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="IVO69_001856"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7400626.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="IVO69_001857"
     CDS             14909..16156
                     /locus_tag="IVO69_001857"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7400627.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="IVO69_001858"
     CDS             16232..16576
                     /locus_tag="IVO69_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400628.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="IVO69_001859"
     CDS             complement(16693..17019)
                     /locus_tag="IVO69_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400629.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4701 a         3704 c         3157 g         5710 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac gg
//
LOCUS       DACCPY010000064        17008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCPY010000064 DACCPY010000000
VERSION     DACCPY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.065"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..127)
                     /locus_tag="IVO69_001860"
     CDS             complement(<1..127)
                     /locus_tag="IVO69_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7400630.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IVO69_001861"
     CDS             complement(231..542)
                     /locus_tag="IVO69_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7400631.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IVO69_001862"
     CDS             complement(544..741)
                     /locus_tag="IVO69_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7400632.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IVO69_001863"
     CDS             complement(1135..2862)
                     /locus_tag="IVO69_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7400633.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IVO69_001864"
     CDS             complement(2855..4048)
                     /locus_tag="IVO69_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7400634.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IVO69_001865"
     CDS             complement(4235..4879)
                     /locus_tag="IVO69_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7400635.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IVO69_001866"
     CDS             complement(4973..5107)
                     /locus_tag="IVO69_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7400636.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IVO69_001867"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IVO69_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7400637.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IVO69_001868"
     CDS             complement(7238..7711)
                     /locus_tag="IVO69_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7400638.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IVO69_001869"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IVO69_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7400639.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IVO69_001870"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IVO69_001870"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7400640.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IVO69_001871"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IVO69_001871"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7400641.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IVO69_001872"
     CDS             complement(11758..12897)
                     /locus_tag="IVO69_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7400642.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IVO69_001873"
     CDS             complement(13206..13919)
                     /locus_tag="IVO69_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7400643.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IVO69_001874"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IVO69_001874"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7400644.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IVO69_001875"
     CDS             15108..16640
                     /locus_tag="IVO69_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7400645.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="IVO69_001876"
     CDS             complement(16831..>17008)
                     /locus_tag="IVO69_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7400646.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DACCPY010000065        16179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCPY010000065 DACCPY010000000
VERSION     DACCPY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.067"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..783
                     /locus_tag="IVO69_001877"
     CDS             <1..783
                     /locus_tag="IVO69_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7400647.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IVO69_001878"
     CDS             1161..3203
                     /locus_tag="IVO69_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7400648.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IVO69_001879"
     CDS             3214..3834
                     /locus_tag="IVO69_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7400649.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IVO69_001880"
     CDS             3846..4304
                     /locus_tag="IVO69_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400650.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IVO69_001881"
     CDS             4323..4601
                     /locus_tag="IVO69_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400651.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IVO69_001882"
     CDS             4626..5993
                     /locus_tag="IVO69_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7400652.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IVO69_001883"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IVO69_001883"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP7400653.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IVO69_001884"
     CDS             7180..7671
                     /locus_tag="IVO69_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400654.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IVO69_001885"
     CDS             8114..9313
                     /locus_tag="IVO69_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7400655.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IVO69_001886"
     CDS             complement(9808..10566)
                     /locus_tag="IVO69_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7400656.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IVO69_001887"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IVO69_001887"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7400657.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IVO69_001888"
     CDS             11666..13111
                     /locus_tag="IVO69_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400658.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IVO69_001889"
     CDS             13139..13594
                     /locus_tag="IVO69_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7400659.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IVO69_001890"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IVO69_001890"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7400660.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IVO69_001891"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IVO69_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IVO69_001892"
     CDS             15403..16017
                     /locus_tag="IVO69_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400661.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         5058 a         2680 c         3439 g         5002 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattt tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgt
//
LOCUS       DACCPY010000066        16130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCPY010000066 DACCPY010000000
VERSION     DACCPY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.068"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IVO69_001893"
     CDS             <1..387
                     /locus_tag="IVO69_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7400662.1"
                     /translation="DTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTS
                     SDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNN
                     WITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(542..1528)
                     /locus_tag="IVO69_001894"
     CDS             complement(542..1528)
                     /locus_tag="IVO69_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7400663.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1551..2117)
                     /locus_tag="IVO69_001895"
     CDS             complement(1551..2117)
                     /locus_tag="IVO69_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7400664.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2131..2547)
                     /locus_tag="IVO69_001896"
     CDS             complement(2131..2547)
                     /locus_tag="IVO69_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7400665.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3004..4938)
                     /locus_tag="IVO69_001897"
     CDS             complement(3004..4938)
                     /locus_tag="IVO69_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7400666.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5240..6175
                     /locus_tag="IVO69_001898"
     CDS             5240..6175
                     /locus_tag="IVO69_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7400667.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6265..7368
                     /locus_tag="IVO69_001899"
     CDS             6265..7368
                     /locus_tag="IVO69_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7400668.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7381..8334
                     /gene="menA"
                     /locus_tag="IVO69_001900"
     CDS             7381..8334
                     /gene="menA"
                     /locus_tag="IVO69_001900"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7400669.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9148..10494)
                     /gene="gor"
                     /locus_tag="IVO69_001901"
     CDS             complement(9148..10494)
                     /gene="gor"
                     /locus_tag="IVO69_001901"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7400670.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10628..11869)
                     /locus_tag="IVO69_001902"
     CDS             complement(10628..11869)
                     /locus_tag="IVO69_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7400671.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11887..12810)
                     /locus_tag="IVO69_001903"
     CDS             complement(11887..12810)
                     /locus_tag="IVO69_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7400672.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13031..15523)
                     /locus_tag="IVO69_001904"
     CDS             complement(13031..15523)
                     /locus_tag="IVO69_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7400673.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15530..15997)
                     /locus_tag="IVO69_001905"
     CDS             complement(15530..15997)
                     /locus_tag="IVO69_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7400674.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4735 a         3367 c         2868 g         5160 t
ORIGIN      
        1 gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag tgatagcacg
       61 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
      121 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
      181 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
      241 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
      301 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
      361 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
      421 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
      481 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
      541 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
      601 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
      661 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
      721 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
      781 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
      841 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
      901 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
      961 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
     1021 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
     1081 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
     1141 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
     1201 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
     1261 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
     1321 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
     1381 acagctttcc gccggcatgg atcgtttctt tgatggcttg ttcgacaggt ttgtttttca
     1441 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
     1501 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
     1561 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
     1621 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
     1681 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
     1741 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
     1801 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
     1861 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
     1921 agtcgtagcc aaagtagtaa gaagctgtct tgttttttca tggtaaaaat ggcaacgatc
     1981 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
     2041 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
     2101 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
     2161 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
     2221 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
     2281 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
     2341 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
     2401 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
     2461 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
     2521 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
     2581 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
     2641 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
     2701 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
     2761 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
     2821 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
     2881 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
     2941 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
     3001 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
     3061 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
     3121 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
     3181 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
     3241 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
     3301 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
     3361 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
     3421 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
     3481 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
     3541 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
     3601 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
     3661 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
     3721 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
     3781 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
     3841 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
     3901 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
     3961 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
     4021 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
     4081 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
     4141 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
     4201 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
     4261 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
     4321 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
     4381 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
     4441 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
     4501 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
     4561 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
     4621 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
     4681 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
     4741 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
     4801 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
     4861 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
     4921 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
     4981 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
     5041 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
     5101 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
     5161 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
     5221 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
     5281 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
     5341 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
     5401 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
     5461 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
     5521 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
     5581 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
     5641 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
     5701 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
     5761 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
     5821 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
     5881 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
     5941 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
     6001 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
     6061 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
     6121 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
     6181 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
     6241 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
     6301 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
     6361 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
     6421 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
     6481 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
     6541 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
     6601 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
     6661 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
     6721 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
     6781 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
     6841 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
     6901 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
     6961 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
     7021 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
     7081 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
     7141 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
     7201 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
     7261 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
     7321 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
     7381 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
     7441 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
     7501 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
     7561 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
     7621 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
     7681 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
     7741 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
     7801 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
     7861 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
     7921 gatttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
     7981 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
     8041 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
     8101 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
     8161 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
     8221 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
     8281 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
     8341 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
     8401 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     8461 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     8521 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
     8581 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
     8641 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
     8701 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
     8761 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
     8821 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
     8881 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
     8941 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
     9001 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
     9061 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
     9121 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
     9181 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
     9241 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
     9301 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
     9361 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
     9421 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
     9481 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
     9541 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
     9601 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
     9661 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
     9721 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
     9781 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
     9841 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
     9901 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
     9961 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
    10021 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
    10081 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
    10141 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
    10201 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
    10261 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
    10321 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
    10381 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
    10441 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
    10501 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
    10561 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
    10621 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
    10681 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
    10741 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
    10801 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
    10861 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
    10921 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
    10981 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
    11041 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
    11101 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
    11161 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
    11221 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
    11281 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
    11341 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
    11401 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
    11461 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
    11521 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
    11581 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
    11641 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
    11701 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
    11761 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
    11821 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
    11881 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
    11941 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
    12001 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
    12061 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
    12121 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
    12181 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
    12241 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
    12301 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
    12361 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
    12421 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
    12481 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
    12541 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
    12601 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
    12661 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
    12721 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
    12781 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
    12841 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
    12901 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
    12961 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
    13021 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
    13081 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
    13141 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
    13201 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
    13261 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
    13321 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
    13381 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
    13441 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
    13501 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
    13561 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
    13621 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
    13681 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
    13741 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
    13801 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
    13861 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
    13921 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
    13981 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
    14041 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
    14101 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
    14161 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
    14221 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
    14281 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
    14341 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
    14401 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
    14461 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
    14521 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
    14581 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
    14641 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
    14701 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
    14761 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
    14821 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
    14881 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
    14941 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
    15001 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
    15061 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
    15121 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
    15181 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
    15241 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
    15301 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
    15361 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
    15421 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
    15481 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
    15541 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
    15601 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
    15661 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
    15721 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
    15781 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
    15841 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
    15901 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
    15961 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
    16021 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
    16081 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACCPY010000067        15937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCPY010000067 DACCPY010000000
VERSION     DACCPY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.069"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(174..476)
                     /locus_tag="IVO69_001906"
     CDS             complement(174..476)
                     /locus_tag="IVO69_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400675.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IVO69_001907"
     tmRNA           complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IVO69_001907"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1233..2006)
                     /locus_tag="IVO69_001908"
     CDS             complement(1233..2006)
                     /locus_tag="IVO69_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7400676.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2150..2500
                     /locus_tag="IVO69_001909"
     CDS             2150..2500
                     /locus_tag="IVO69_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7400677.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2481..3197
                     /locus_tag="IVO69_001910"
     CDS             2481..3197
                     /locus_tag="IVO69_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400678.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3197..4645
                     /locus_tag="IVO69_001911"
     CDS             3197..4645
                     /locus_tag="IVO69_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7400679.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4715..5224)
                     /locus_tag="IVO69_001912"
     CDS             complement(4715..5224)
                     /locus_tag="IVO69_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400680.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5214..5939)
                     /locus_tag="IVO69_001913"
     CDS             complement(5214..5939)
                     /locus_tag="IVO69_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400681.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6162..8282)
                     /locus_tag="IVO69_001914"
     CDS             complement(6162..8282)
                     /locus_tag="IVO69_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7400682.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8512..9690
                     /locus_tag="IVO69_001915"
     CDS             8512..9690
                     /locus_tag="IVO69_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7400683.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9706..10464
                     /gene="aroD"
                     /locus_tag="IVO69_001916"
     CDS             9706..10464
                     /gene="aroD"
                     /locus_tag="IVO69_001916"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7400684.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10487..10972
                     /gene="ybaK"
                     /locus_tag="IVO69_001917"
     CDS             10487..10972
                     /gene="ybaK"
                     /locus_tag="IVO69_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7400685.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11043..12296)
                     /locus_tag="IVO69_001918"
     CDS             complement(11043..12296)
                     /locus_tag="IVO69_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7400686.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12413..13762
                     /gene="gdhA"
                     /locus_tag="IVO69_001919"
     CDS             12413..13762
                     /gene="gdhA"
                     /locus_tag="IVO69_001919"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7400687.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13874..15220
                     /locus_tag="IVO69_001920"
     CDS             13874..15220
                     /locus_tag="IVO69_001920"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7400688.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15528..15914)
                     /locus_tag="IVO69_001921"
     CDS             complement(15528..15914)
                     /locus_tag="IVO69_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7400689.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5216 a         2844 c         3192 g         4685 t
ORIGIN      
        1 acttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
       61 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatcccttt
      121 tataagtatg gtgataaaat ccgttccggg ctaccttttt taggtagcct actttaatct
      181 ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg atgatcgctc
      241 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
      301 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      361 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      421 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      481 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      541 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      601 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaataag
      661 gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg gagtcgaacc
      721 cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc
      781 actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca
      841 acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat
      901 gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt
      961 ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg
     1021 caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta
     1081 cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg
     1141 aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt
     1201 cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg
     1261 tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg
     1321 tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc
     1381 ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt
     1441 ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc
     1501 cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag
     1561 atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct
     1621 gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc
     1681 tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga
     1741 cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa
     1801 ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct
     1861 tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct
     1921 tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag
     1981 aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat
     2041 cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc
     2101 agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt
     2161 cagtattggt atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg
     2221 aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg
     2281 gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat
     2341 cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct
     2401 agtatttcca attatgccta tttttattat gacagaaaag aaattacaga aatatgacct
     2461 ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg
     2521 aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa
     2581 aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc
     2641 tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta
     2701 ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag
     2761 agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa
     2821 tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca gtagtttcag
     2881 atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt
     2941 atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt
     3001 tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt
     3061 atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa
     3121 tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg
     3181 attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct gcggaatggt
     3241 cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga
     3301 agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac
     3361 ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct
     3421 gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg
     3481 tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa
     3541 aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc
     3601 acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact
     3661 tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct
     3721 gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc
     3781 attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa
     3841 ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt
     3901 caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg
     3961 tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa
     4021 cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat
     4081 taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta
     4141 tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac
     4201 attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac
     4261 attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat
     4321 ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt
     4381 agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga
     4441 acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac
     4501 aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa
     4561 aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc
     4621 tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt
     4681 ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa
     4741 tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt
     4801 catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat
     4861 caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca
     4921 gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga
     4981 tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca
     5041 acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg
     5101 caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct
     5161 gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt
     5221 tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga
     5281 ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata
     5341 tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga
     5401 attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt
     5461 aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc
     5521 cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa
     5581 aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc ctgcttgttt
     5641 tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg
     5701 aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat
     5761 gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa
     5821 tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag
     5881 agtattgtct gctttttgta ataaattaat cgagcagtag catagcggat tattttcata
     5941 cttttcacct ctataaaaag tatagaaaat gaatatgaat aaagtatgaa attcctttag
     6001 tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg tgaagaagct
     6061 gccctattct attaccgtag atgcaagctt ttgaacactg tttattcgaa aagaggagca
     6121 gcgacaataa ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg
     6181 gtcgttccgt gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg
     6241 ttgtgttcac taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat
     6301 ttctctaagc cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc
     6361 gtatagaacc gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca
     6421 actttttctt tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct
     6481 tcaccagttt cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa
     6541 tcaccatcac ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac
     6601 tgaatataat tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact
     6661 tgtccgccgt aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa
     6721 gggacacgct gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact
     6781 aattcagcta gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg
     6841 atgactgaat tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg
     6901 gtatctaaat aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct
     6961 aacgggaatc ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca
     7021 atgaattttg aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa
     7081 aatggtttat catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa
     7141 gaatcaaaga aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta
     7201 ccagcgttcc catggaatac cgcactaatg taaccttgtg tttgacttaa aatatcaggt
     7261 gcagcttgga aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca
     7321 aacaatgaat tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg
     7381 aaaaagttgt caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta
     7441 acttcatgct ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag
     7501 ctttctaaat ggatataaat cacatttttt cctctagcga gcccgaacaa atcgtcattt
     7561 ggtgctgcat aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct
     7621 tctgcccgca cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc
     7681 aagccaagat atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga
     7741 tcagcttccg ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca
     7801 aaagccattc ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga
     7861 acagtcaaga taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg
     7921 gcactttctc ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg
     7981 aagtccgtga attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa
     8041 taaatgacca tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca
     8101 agtaataaca atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt
     8161 tcgattccga gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg
     8221 actagtaaag aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc
     8281 aagataatct tcctttctgc acttcttaat cacagaagtg tcctgaaacc atgttacaca
     8341 gctcacttat tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag
     8401 tttatgctgt ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag
     8461 tcctatgttt tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta
     8521 aaagtaaaaa aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa
     8581 gaccttgctc atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac
     8641 caatttatcg caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc
     8701 cagcagaaca tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa
     8761 aaaagaactt tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa
     8821 ggagacggga tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg
     8881 tataacgaaa cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac
     8941 ccggaaatca taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc
     9001 cagttcattt acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa
     9061 tttgccgttt atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta
     9121 cggggatcat tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat
     9181 accggcgcct tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat
     9241 ctagcaaaac gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc
     9301 gatcagcaaa aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa
     9361 caatttttat atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa
     9421 gtctttcgcg tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca
     9481 gaagacggga tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa
     9541 acaatgattg aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat
     9601 cgtctgccaa aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg
     9661 tttgtttatc aaattaaaaa taaccattag aaggagcgat gcagaatggt tcgggtaaag
     9721 ggattgaata taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga
     9781 gaggaagtat tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg
     9841 cgagtcgatc attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt
     9901 atccatgaga tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc
     9961 ggagaacaag acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca
    10021 ggattagtag atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg
    10081 atagaggaaa tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa
    10141 acaccagcag accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat
    10201 atcggaaaac tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg
    10261 actagacggg caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt
    10321 ggtaaaattt ctcgtctggt tggcggaacg acgggttccg ttatgacatt tggttcctta
    10381 agcagcgatt ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt
    10441 caattattaa atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa
    10501 aatagtaaaa acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca
    10561 cgaatatgag tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga
    10621 aagccaatct cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt
    10681 tgctgtgata ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa
    10741 taaaaaagta gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg
    10801 tggctgctcg cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga
    10861 aagctatgaa caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc
    10921 tcaagatatc tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg
    10981 tataaaagaa agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata
    11041 cattatgctc tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg
    11101 atattaaagc cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca
    11161 tcaatgattt tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca
    11221 gttacaaatg atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat
    11281 agttgaacaa aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt
    11341 tcaagccgct caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg
    11401 accaagttct ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg
    11461 gtttgtgtag ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct
    11521 ttattgacaa aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa
    11581 agtaaatcca tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg
    11641 atgtcttgta aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc
    11701 aagggggact ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg
    11761 tacccatctc cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg
    11821 gcaggagcat ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg
    11881 atcaatttct ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg
    11941 agcaatgctt cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc
    12001 tcttctttta aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat
    12061 tgcgcaaacg tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca
    12121 acaggacgat tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct
    12181 gcttttttgt ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct
    12241 gccatcatcc cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc
    12301 tcacctcaag gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat gttttgagcc
    12361 aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa
    12421 cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga
    12481 agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca
    12541 tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca
    12601 atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat
    12661 ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa
    12721 tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct
    12781 gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga
    12841 gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat
    12901 tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga
    12961 atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg
    13021 gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct
    13081 attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa
    13141 tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc
    13201 agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt
    13261 caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta
    13321 tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc
    13381 tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat
    13441 cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc
    13501 aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt
    13561 agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct
    13621 ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga
    13681 cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat
    13741 ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt
    13801 ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata
    13861 aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa
    13921 cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga
    13981 aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa
    14041 agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt
    14101 agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa
    14161 tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc
    14221 cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga
    14281 cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg
    14341 tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta
    14401 cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga
    14461 aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt
    14521 gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc
    14581 aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat
    14641 gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg
    14701 aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca
    14761 aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt
    14821 catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa
    14881 atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa
    14941 agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg
    15001 tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac
    15061 aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt
    15121 cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg
    15181 ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga
    15241 tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg
    15301 tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca
    15361 gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt
    15421 aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa
    15481 gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct
    15541 aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc
    15601 cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca
    15661 gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa
    15721 gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca
    15781 gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac ataagtagac
    15841 tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct
    15901 aaaatttttt tcatgaaaga tctccttttt tattttt
//
LOCUS       DACCPY010000068        15368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCPY010000068 DACCPY010000000
VERSION     DACCPY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.070"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(98..1393)
                     /gene="purB"
                     /locus_tag="IVO69_001922"
     CDS             complement(98..1393)
                     /gene="purB"
                     /locus_tag="IVO69_001922"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7400690.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1406..2539)
                     /gene="purK"
                     /locus_tag="IVO69_001923"
     CDS             complement(1406..2539)
                     /gene="purK"
                     /locus_tag="IVO69_001923"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7400691.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMGQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2523..3011)
                     /gene="purE"
                     /locus_tag="IVO69_001924"
     CDS             complement(2523..3011)
                     /gene="purE"
                     /locus_tag="IVO69_001924"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7400692.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3427..4734)
                     /locus_tag="IVO69_001925"
     CDS             complement(3427..4734)
                     /locus_tag="IVO69_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7400693.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4737..5318)
                     /locus_tag="IVO69_001926"
     CDS             complement(4737..5318)
                     /locus_tag="IVO69_001926"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7400694.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5434..5531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5890..7182)
                     /locus_tag="IVO69_001927"
     CDS             complement(5890..7182)
                     /locus_tag="IVO69_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7400695.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7179..7802)
                     /locus_tag="IVO69_001928"
     CDS             complement(7179..7802)
                     /locus_tag="IVO69_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400696.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7805..8641)
                     /locus_tag="IVO69_001929"
     CDS             complement(7805..8641)
                     /locus_tag="IVO69_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7400697.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8658..9545)
                     /locus_tag="IVO69_001930"
     CDS             complement(8658..9545)
                     /locus_tag="IVO69_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400698.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9561..10844)
                     /locus_tag="IVO69_001931"
     CDS             complement(9561..10844)
                     /locus_tag="IVO69_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7400699.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11527..12210)
                     /locus_tag="IVO69_001932"
     CDS             complement(11527..12210)
                     /locus_tag="IVO69_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7400700.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12228..12845)
                     /locus_tag="IVO69_001933"
     CDS             complement(12228..12845)
                     /locus_tag="IVO69_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7400701.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12855..13679)
                     /locus_tag="IVO69_001934"
     CDS             complement(12855..13679)
                     /locus_tag="IVO69_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7400702.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13672..14232)
                     /locus_tag="IVO69_001935"
     CDS             complement(13672..14232)
                     /locus_tag="IVO69_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400703.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14406..15362
                     /locus_tag="IVO69_001936"
     CDS             14406..15362
                     /locus_tag="IVO69_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7400704.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4514 a         3427 c         2631 g         4796 t
ORIGIN      
        1 ataataaact aaaagaagca gaaccgctaa cggatctgct tcttttttgg ttatttttgc
       61 tattctattt taattgaatt ttgcaactca ctcttcttta ttccaaacct actcgtttga
      121 atatcacgtc aacgtttttc aaatgatgat gataatcaaa ggcatcgtct atttcttctt
      181 tagataatac cgatgtgatg gttggatctt cttctaacag cgggcggaac tgcacttggt
      241 tgtcccaagc gtacgcagtt tttggctgaa caagatcata agcttcttct cttgtcattc
      301 ccttatcgat caatttcagc ataacgcgct ggctataaat caaaccaaat gttgcagaca
      361 tattccgctt catgttttca ggaaagaccg ttagattttt aacgatattg ccaaaacgat
      421 ttaacatgta attcaataaa atcgtggtat caggcaagat gattcgttct gctgaagaat
      481 gagaaatatc tctttcatgc cataatgtga cattttcata ggcagtgacc atgtgtccgc
      541 gcaccactct ggctagacca gccatatttt ctgaaccgat cggattcctt ttgtgcggca
      601 tagcagaaga acctttttga cctttcgcaa aaaattcttc tacttcacgt gtttcggatt
      661 tttgcaatcc gcgtatctct gttgcaaaac gttcaatact tgtagcaata agcgccatcg
      721 ctgagaaata ttcagcatgt agatcccgag ggagaacttg agtagagatt tcttgtggac
      781 ggatccccaa ttgtttacat acatactctt caataaatgg cgggatattt gcgaacgttc
      841 ccactgctcc tgagattttt cctgcttcta ctccttttgc cgcatgttca aatcgttcga
      901 tgttccgttt catttctgaa taccatgtgg caagtttcaa tccaaaagta gttggttcag
      961 catggacacc gtgtgtccga cccatcatca ctgtatattt gtgttctttt gctttttcgc
     1021 caatgatttc cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct tgcttgatga
     1081 gatagccata agcagtatct acgacatccg tacttgttag accataatgg acccattttc
     1141 tttcttctcc caatgtttca gaaaccgctc gtgtaaacgc aacgacatca tggcgtgtta
     1201 atttttcgat ttccaagatc cgatcgatat caaatgatgc atgctcacga atttttttca
     1261 catcttcttg cgggatttca ccaagttctg cccaagcttc atctgctaaa atctcgactt
     1321 cgagccacgc acggtatttg ttctcttccg tccagatatt ccccatttca gggcgtgtat
     1381 aacgttctaa catgtttttt ctcctctaat cccaaatatt cgtttgtttg atttcttcca
     1441 gcgtttgctg gatgtcttct gtcaagatag tgatatgtcc catcttgcga cctttttttg
     1501 cttgtttttt tccatagaaa tggaattgcc agtctggctt attctcaatc aagcgatagc
     1561 tttccagcag ttgttctccc aaaacattga ccattactgc attgcttatc aaacgtactt
     1621 tttctcctaa tggccaacca caaatacctc gaatatgggc atcgaactga cccatagaac
     1681 aagcttcaat agaataatgt ccagaattgt gaggacgagg agctaattca ttcacatata
     1741 gcccgcctgt ttttgttaaa aacatttcta cccctaaaac acctgaaagg ctcactgctt
     1801 ctgcaatcac acgggcaata cgctcagcct cctcgatcac atcctgatca atcgcggctg
     1861 gagcaatcgt ttcatgaaga atattgttgt ggtggatgtt ttccacgacc ggaaaagtcg
     1921 tgaaatctcc ttgaccattt cctgccacca tgatcgaaat ctctttttca aacgggatcc
     1981 atgcttctaa aacacaggtt ccttctctta aaaggtccat ggcaggagct agatccgccc
     2041 gacttttcaa aacatactgc cccttgccat catagccgcc tcgagtggtt ttcaaaacac
     2101 aaggatagcc gatcccgtca atcgcatctt ggatatccgt cggactgacg atcgttgcat
     2161 aaggagcaat cacgatgtta ttcgtttcca aaaaagattt ttctaacagg cggtcttgcg
     2221 taatcgcaag caagtctgtc ccttgaggaa taaatgacat gggtaaaatc gcatttaacg
     2281 cctctacact gacgttttcg aattcatagg taatgacatc tgttcttctg gcaagctctt
     2341 tcagcgcaaa ggtgtcatca tagacacttt caatgtgcca atctgcaact tgtgcggctg
     2401 gacaatcgct cacaggatcc agcacaccta ctttaaagcc catttctttt gcactaatcg
     2461 ccatcatacg acctaattga ccgccaccga cgatcccgat cgttgaacca ggcaacaaag
     2521 gtttaaccaa gttgatcact actttctata acagttttag ccaacattga acgtctttca
     2581 gccaatttag cctccaattc taaatcatac atggaaagca tctgtatcgc aagtagtcca
     2641 gcattaattg ctcccgcttt tccgattgct gtagtagcta caggtactcc tccaggcatt
     2701 tggacaatcg aaagaagcga atccaaacca tttaatgttc gtgattggac cggtacaccg
     2761 ataaccggca aagttgtttt tgatgcgacc atacccggca gatgcgcggc tccccctgct
     2821 cctgcaataa tgattttgat tccttttttt cgagcgtttt gtgcatattc aaacatcaaa
     2881 tctggggttc gatgagcaga aacgaccttt ttttcataag acacaccgaa ttcatccaat
     2941 atttgacatg cttctttcat tgtttcccaa tctgaagtac tccccataat cacagagatt
     3001 tttgctgtca ttgtttttcc tcccagtttt tctacattca tattttaaca atcacttttt
     3061 ttgatgtcaa agaaatttcg aatgttaaca aagaaaaaca ttttattgtt cgtgtttatt
     3121 aaagataaaa aaaaggaatg tgacaaaagt taggtcacat tcctttttat ggataaacgg
     3181 tgttctcctg ccaactcctc ggtcacaagg aacaatattc aacaaacatg agaagctttt
     3241 ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct cttccttcaa
     3301 cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat acgaggaact
     3361 atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa cctttctttt
     3421 gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca atccattaaa
     3481 gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc cagtgaacat
     3541 ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac ccagcccaaa
     3601 tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa cttccaacag
     3661 catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc caccaagtac
     3721 cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta aaacaatcaa
     3781 gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt gaaccaatcc
     3841 gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta agataacggc
     3901 aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt tcccagtaat
     3961 gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg aaatgatgat
     4021 cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg gttgtggaac
     4081 atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga aagcagctaa
     4141 gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc cttttgccag
     4201 catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat ttgttttgtc
     4261 accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta caggaatcaa
     4321 agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata gttttttaat
     4381 tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa tagatccgta
     4441 gatagctcct actccttcat tggttccgat caagatcagt ggagctactg cttggatcgc
     4501 acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca attgcaataa
     4561 tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct gttcttgatt
     4621 aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat acatcgctag
     4681 cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt ccacgattat
     4741 gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg cgcaagagaa
     4801 acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga cttttcgatg
     4861 acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat caatcctttt
     4921 gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc tgaagataaa
     4981 aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata aacagaagct
     5041 gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat cattggaaca
     5101 tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat ggtcacaact
     5161 ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg ttccatcaat
     5221 acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc gcctaatata
     5281 cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc tttatcattt
     5341 attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt aaagaaaaac
     5401 accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac ttatagtcca
     5461 gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata agtaggtatg
     5521 taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg aaggcagttt
     5581 gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca ctactttctg
     5641 aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta atgcattatt
     5701 cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa agcagcccac
     5761 ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata aatagtgttc
     5821 aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt aatttccgta
     5881 aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt ttcctgaagc
     5941 aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc
     6001 tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta ataaacgcgg
     6061 atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa
     6121 catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt tatctccacc
     6181 tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc tgccattgaa
     6241 agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca aaatcgcacc
     6301 agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat cggaaaatac
     6361 actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt ctcgcaattc
     6421 ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc ttccatagca
     6481 tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa
     6541 tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca ctagatcaaa
     6601 atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc gatgccatct
     6661 tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga tagaaaacac
     6721 atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg gtgcataacg
     6781 ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg
     6841 agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc cttttttcat
     6901 tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga cttcagccgg
     6961 ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta cttttttctg
     7021 tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag atacatcaca
     7081 aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat aaaaaaaacg
     7141 cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc attctccttt
     7201 tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc agtgagtaaa
     7261 aaatcatgct gtatacacca gaacagtcca tacaaaaggc ataaatacca cagaaacccc
     7321 ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag actatctgct
     7381 ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac aaaaagcaac
     7441 agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa taaataccaa
     7501 tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca catccgtttg
     7561 atttttcctt tcctccaaca tacctttttt tctgccaagt aatggacaga tagataataa
     7621 gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag cactaatcct
     7681 aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag actggttaaa
     7741 aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat tggctttttc
     7801 atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta ttgaagaata
     7861 agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata ctcgcgtaag
     7921 tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg taagatctcg
     7981 gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa aactggatga
     8041 tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta aagacactat
     8101 acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg ctgaccatga
     8161 attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca
     8221 catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc aaaatcactt
     8281 gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt cctttgaaat
     8341 cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct atcgtcgaaa
     8401 ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg
     8461 cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc agcgaaccac
     8521 tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc ggataaatca
     8581 tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact gtattattca
     8641 tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa aatcagaaag
     8701 gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata ccccatagaa
     8761 ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc atcattcgga
     8821 ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat tgtctgcata
     8881 accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc
     8941 catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat ctctttgata
     9001 cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa aacaagaggg
     9061 atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg
     9121 aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc gatagaaata
     9181 ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt tcctttgata
     9241 ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg tacagatgca
     9301 aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact cagcatcttt
     9361 atataattat ccaagccgat ccatgagact ggatttccca aacgataatc ggtgaacgaa
     9421 taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag gatgaatgga
     9481 gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag tttttctttt
     9541 ttcatgcctt cctcctgctg ctatcctaga atgtttgacg caaactcata aagactctct
     9601 gctgcttctt ctgctgttac ttcttcataa atgacttttt caactaaaag attgtattgg
     9661 tcttgaattt ccggattcaa agcactatca aagacattca cttcgccgtt gtccatatcg
     9721 gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg catcatctgc acgagcgagc
     9781 tgttctcgaa tattcgacat gattggtaca ccacgttctc cttttaaaat ttcattggct
     9841 tcttcactgt tttgaaagaa atcaataaac ttagcagctt cttctttatg ggctgagctt
     9901 tttgccatac tcagcatttg cgaagaacgt aaagggatac cagattctcc atctgctttt
     9961 ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag gagcccctga caatatttct
    10021 gtcaactggt tacttgatag aaacaccatt gccgcttctc tatccctaac cgcctgaaaa
    10081 gacagatccg tcgtacttgc agctccacca cctggttctg gatgggcccc tttgtctacc
    10141 agatttttac gcagctggaa aaaatctgtt agatagtctg gattttcaaa tcccaagctg
    10201 ttagatagat cttcccgatt ataaaaattc aatcctttct caacttgtgg tatttgaata
    10261 atacagccag ctataaaatc ttcgaatctt gaaatagcat agattcctaa ttcttcagtg
    10321 atttgatagc tgatttgttc aaattcatcc catgtccaat ttgaagctgg ttcagggata
    10381 tttaattgtt caaatatatt tttattgtaa gcaatcgcaa acgaattcac cccgttagac
    10441 agtccaacta attgcccgtc gtaagtcgtc gttgctaaat aggattcatc tgcattagaa
    10501 atatcaatta tcccttgctc cacatattca tctaatggtt caatctgatt gatgtattga
    10561 ggaaattgat tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct
    10621 aattttgtgt aatatccttc ataatcataa aattcataat cgatcgtcac atggggattt
    10681 ttttcttcat aaagctcgat tacttttact gttgcatcgt gtcggacttg cgaaccccac
    10741 caagccatgt tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt caagaagaat
    10801 aacataacac ttaaagctaa gatgattctc attcttttct tcattctctg cacctctact
    10861 tccattgtta tttttcaata tactgtattt ttatacagaa catatttatt aaacgatctt
    10921 tttcaagggg aataaaaacc aagacttgcc ttatcgttct gcctaggatg aacaactcaa
    10981 tactttcaag aaaaattaga tagtgctgat tcttctttca tcgctaaaaa cacttcttgc
    11041 ttcaatgatg ggaggataat tacgcaaaga aaaaaggata tcagacaact gcccaatatc
    11101 cttgacatag ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa
    11161 taactagtaa ttctgtatga aattaggtac aatatacacg tcgatgcaca agcattgact
    11221 atggcaactt gattggtcag ttcccgctga cttttcaagc cccgacaatt tagctgctac
    11281 taaattgtcg ggtgcctttt ttagttttta ttcgtttttc attctataga aagcgcttca
    11341 ataaatcaag acattttttt gttagaatgc taataataac aaaaggtttt aactcttctt
    11401 atgtacatta caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc tgaacaaata
    11461 gtactggagt acaaaagacg aggaagagcc actatctgct cttcctcgtc ttttgattct
    11521 cacctgttat tcatgattct ttttctcagt ttcccgatat ttttccattt tacgactaaa
    11581 aagctctccg ctacttggtg gcgtatatgt gatggcattt gctcctgctt gaatggtctc
    11641 agcaatactt tcttccgttg gaccacctgt cgcaatgata ggaagttcag gatatttttt
    11701 acgaaaatgg cgtactgctt ctgcagtttc tttacctgca ctgatattga taatcgaaat
    11761 accagctgct aacttttcat ctatttctgt atatttcgac gttaccgtac tgataactgg
    11821 aatatcaact actcgacaaa cttcttttac tgtctctaaa ggcgtaggac cgttcaaaac
    11881 gacccctaac gaaccttgcg cttctgcgaa catgctcatg taggaagaac gagcaccttg
    11941 tgtcaaacca ccacctacac cggagaaaac tggaatgtca gctgcctcaa taatgctttt
    12001 agtaatcgct ggatgaggtg taaaaggata cacagcaatt actgcattcg catttgtatt
    12061 acgtatgatc gcaatatccg tagtaaagat gatcgaacgg atcaattttc caaatatctt
    12121 gatcccgctt gcttctaaaa tgatttccgg tactcttacg atatcttttc tcaattccgt
    12181 catgatttct ggcgtaaatt gttctgccat cagccacttc tcctttgcta tctccggtca
    12241 tactgattca attgatacgt ctcgatgacc tggatgattt tttgcgcata accggggtct
    12301 gtcgcataac ctgcatcttg caatgcttgt gcggcttcct gatagttggt ggccgtaatc
    12361 actttttcat acagcgcagg attccaatct actccttgga cgaacagttg tgtatggtca
    12421 tccatggact gttcccatga atcatacact ttgaagtctc cttggatagt tatccattct
    12481 tcgttaacaa attcctttgt ttccaggctt actttcttct gatccccata agccttgatc
    12541 ccgaaaagat tattgtattt gctagccaag gtactttttc cccaatttga ttctaatata
    12601 gcttgtccta aaatgatact aggaagcact ccataaccat cttgtagttc ttgggcatgc
    12661 ggaacaagcc gttcaataaa ttgctgatgg gattgcgctt cattttcttg ctgattatca
    12721 gtaaaaggag aagacagatt cttcaaggaa aaaacaaagg ctaagagtat cactgctaca
    12781 ccggcaacca ctgctggcca acggatattc ttgcgctttt gctttttcca cttttttttc
    12841 gccatgatca attcctactt ttcatttaat tcattcagat attcttctag cgtcaattgg
    12901 tgtttggtga tatactcagc gttttcttta ccgacataac gtaagtgcca aggttcatat
    12961 gtgatcttag ttatatcttc gtgtcctttt ggataacgaa cgataaatcc atactgcgca
    13021 gcattatctg cgatccattt agctccaggc tgatctccgt aacttgcatc caatacagta
    13081 gaaggataac tgttgtacca gttttgatcg acgacatcga ctgccagacc tgtgtggtgt
    13141 tcgctgtatc ctggctccgt aatcgtttct ttagtaatct ttgtcgcctc ctcttctgat
    13201 acaccttgac gactcatcac atcttggaca ttttgttcga aaacttgttg ttgggatgat
    13261 acagaccggt aagctgagac catcactaat ggaaatccag ctgcttcagc agccttggca
    13321 aatgcttcat attctggagc aatgcgtttg tcgatcaagt atccgttact taatgatgcc
    13381 aattgagtag actcatcaat ttctttttct aatttgtgat ctggaccaac caaaactagc
    13441 ttccaatcgt ctgttttaac atctggcaat tgtttggacg tacgatcttt tgtttctttg
    13501 tcctttacct tgctgctggt tgtttgactg ttcttttctt tactagcagt tgttttggtg
    13561 cttgttcgtt cgactgcttg attgccggct tgaacatgcg aatcatcatc gctaaaaata
    13621 acatagccca ttgctgcgat cataatcaca actgctccaa atcctaaaaa tttattcact
    13681 tgaaaaatcc tttcgttgtt cttcacgttc cccattcgcc tcatgaacgt aagtttcttc
    13741 cagattttca ttcacccaat cgagaagttc gattttttct ccttcgatct gtttttgaac
    13801 atctgctggc agacgaaaat tcaaaatatc atcatagccc cattcatcat attgaatact
    13861 tactttcaag tcaaggtatc taccagcctg ctgattattt ttcaaaggga tcagctcgac
    13921 ttttgctgct ttgctgctaa gccatttttg cgcgatgcgt gttttgattt gcttgtactc
    13981 tgcaacggca acgtttcgtt tttcgggaaa aataatcgtt tgacgcaaaa tcttttgcgt
    14041 tagcatccat tcataatcag tgaaaagatt ttttactttc tgcatcgctt cagatggcag
    14101 atttttctcg ccatctttcc atttttgcca ttgttctgga ctgatcagca agtattcatc
    14161 taaaaaattt ttttcagttt caaactgttc taataataca ctcataataa gatcaatata
    14221 tacttgatgc acgtttatcc cctcctttta actattctac ttaagttttg atcaaaaagc
    14281 acctcttaaa tcgctgcttt catttttttt aatgtttcaa gtcctctcct ctttattctt
    14341 cgtgattttt gttataataa tagacagaca agaaactcaa tccaactatg aaggagcgtt
    14401 ttcagatgaa aaaatatcaa tggggaatcg ttggtcttgg taccatcgca catgagtttg
    14461 ccgaaagctt caatcaagag acgagcgaac taacagcagt tgcttccaga acttcagaaa
    14521 aagcggaaaa cttcgctcac cgttacaata ttccaaaagc ttacggaagc tatcaagaaa
    14581 tgttagacga tgctgaaatc gacatcgtgt acattgctgt accaaataga cagcatatcg
    14641 accatatctt ggcagcattg gaagcaggca aacacgttct atgtgaaaaa gccatcacga
    14701 tgaataaaaa agaactggct gacgctatgc gcttagcaga agaaaagaat ttgatcttag
    14761 cagaagcgat gacaatcttc aacatgccac tttatcagca gttacgctca ataatggata
    14821 caggaaaact gggggcttta aaaatgatcc aagcaccttt cggcagttac aaagaaccag
    14881 accctaaaaa ccggttcttc aatccagaat tagctggagg agctctgttg gatatcggta
    14941 cgtatgctgt atctttcgct cgtttctttt tgagctctca acctgaagta gttgcctcta
    15001 caatggttcc ttttgaaaca ggcgtggacg aacaatccgt cactatcttg cgtaacaaag
    15061 aaaatgaact tgctgctgtc agcttgactt ttcaggcaaa aatgccaaaa gtcggtatcg
    15121 ttgcctttga gaacggttat gtgacgattg ctgattaccc gcgtgctgat cgtgcggaga
    15181 tcttatttac agatggtaca aaggaattca tcgaaagcgg gaatacatct gaagcaatga
    15241 attacgaaat aaagaatatg gtacacatga ttgaaggtga cttgcccaat cgctctctct
    15301 tcttgacaaa agatgtcatc gaaatcttgg atcaaatgca agttctatgg aaaaatatgt
    15361 aattttaa
//
LOCUS       DACCPY010000069        15295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCPY010000069 DACCPY010000000
VERSION     DACCPY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.071"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(59..2158)
                     /locus_tag="IVO69_001937"
     CDS             complement(59..2158)
                     /locus_tag="IVO69_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7400705.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2378..3487)
                     /gene="rpoD"
                     /locus_tag="IVO69_001938"
     CDS             complement(2378..3487)
                     /gene="rpoD"
                     /locus_tag="IVO69_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7400706.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3511..5352)
                     /locus_tag="IVO69_001939"
     CDS             complement(3511..5352)
                     /locus_tag="IVO69_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7400707.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5534..7927)
                     /locus_tag="IVO69_001940"
     CDS             complement(5534..7927)
                     /locus_tag="IVO69_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7400708.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(8034..8894)
                     /locus_tag="IVO69_001941"
     CDS             complement(8034..8894)
                     /locus_tag="IVO69_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7400709.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(8953..9585)
                     /locus_tag="IVO69_001942"
     CDS             complement(8953..9585)
                     /locus_tag="IVO69_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7400710.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            9794..10513
                     /locus_tag="IVO69_001943"
     CDS             9794..10513
                     /locus_tag="IVO69_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7400711.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            10539..13130
                     /gene="mprF"
                     /locus_tag="IVO69_001944"
     CDS             10539..13130
                     /gene="mprF"
                     /locus_tag="IVO69_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7400712.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(13318..14505)
                     /gene="tuf"
                     /locus_tag="IVO69_001945"
     CDS             complement(13318..14505)
                     /gene="tuf"
                     /locus_tag="IVO69_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7400713.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            14873..15229
                     /locus_tag="IVO69_001946"
     CDS             14873..15229
                     /locus_tag="IVO69_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7400714.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4186 a         3182 c         2710 g         5217 t
ORIGIN      
        1 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
       61 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
      121 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
      181 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
      241 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
      301 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
      361 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
      421 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
      481 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
      541 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
      601 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
      661 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
      721 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
      781 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
      841 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
      901 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
      961 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     1021 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     1081 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     1141 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     1201 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     1261 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     1321 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     1381 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     1441 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     1501 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     1561 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     1621 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     1681 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     1741 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     1801 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     1861 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     1921 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     1981 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     2041 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     2101 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     2161 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     2221 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     2281 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     2341 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     2401 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     2461 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     2521 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     2581 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     2641 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     2701 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     2761 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     2821 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     2881 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     2941 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     3001 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     3061 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     3121 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     3181 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     3241 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     3301 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     3361 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     3421 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     3481 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     3541 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     3601 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     3661 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     3721 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     3781 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     3841 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     3901 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     3961 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     4021 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     4081 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     4141 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     4201 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     4261 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     4321 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     4381 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     4441 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     4501 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     4561 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     4621 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     4681 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     4741 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     4801 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     4861 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     4921 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     4981 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     5041 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     5101 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     5161 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     5221 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     5281 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     5341 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     5401 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     5461 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     5521 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     5581 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     5641 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     5701 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     5761 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     5821 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     5881 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     5941 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     6001 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     6061 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     6121 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     6181 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     6241 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     6301 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     6361 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     6421 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     6481 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     6541 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     6601 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     6661 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     6721 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     6781 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     6841 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     6901 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     6961 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     7021 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     7081 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     7141 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     7201 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     7261 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     7321 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     7381 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     7441 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     7501 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     7561 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     7621 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     7681 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     7741 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     7801 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     7861 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     7921 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     7981 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     8041 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     8101 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     8161 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     8221 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     8281 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     8341 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     8401 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
     8461 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
     8521 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
     8581 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
     8641 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
     8701 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
     8761 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
     8821 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
     8881 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
     8941 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
     9001 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
     9061 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
     9121 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
     9181 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
     9241 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
     9301 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
     9361 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
     9421 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
     9481 taccgaacga taagttcttc ctcctaaaaa caacaccgca ccaataaccg tcagaatgat
     9541 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
     9601 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
     9661 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
     9721 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
     9781 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
     9841 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
     9901 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
     9961 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    10021 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    10081 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    10141 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    10201 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    10261 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    10321 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    10381 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    10441 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    10501 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    10561 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    10621 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    10681 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    10741 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    10801 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    10861 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    10921 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    10981 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    11041 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    11101 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    11161 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    11221 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    11281 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    11341 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    11401 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    11461 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    11521 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    11581 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    11641 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    11701 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    11761 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    11821 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    11881 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccag
    11941 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    12001 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    12061 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    12121 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    12181 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    12241 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    12301 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    12361 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    12421 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    12481 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    12541 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    12601 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    12661 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    12721 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    12781 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    12841 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    12901 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    12961 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    13021 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    13081 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    13141 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    13201 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    13261 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    13321 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    13381 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    13441 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    13501 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    13561 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    13621 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    13681 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    13741 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    13801 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    13861 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    13921 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    13981 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    14041 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    14101 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    14161 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    14221 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    14281 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    14341 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    14401 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    14461 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    14521 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    14581 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    14641 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    14701 tcttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    14761 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    14821 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    14881 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    14941 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    15001 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    15061 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    15121 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    15181 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    15241 aaaattttat tagataaaaa gaggctggga cagaagcgtt taactccaag aaata
//
LOCUS       DACCPY010000070        15072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCPY010000070 DACCPY010000000
VERSION     DACCPY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.072"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..831)
                     /locus_tag="IVO69_001947"
     CDS             complement(<1..831)
                     /locus_tag="IVO69_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400715.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     V"
     gene            complement(860..1177)
                     /locus_tag="IVO69_001948"
     CDS             complement(860..1177)
                     /locus_tag="IVO69_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7400716.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LDA"
     gene            complement(1202..1522)
                     /locus_tag="IVO69_001949"
     CDS             complement(1202..1522)
                     /locus_tag="IVO69_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400717.1"
                     /translation="MEKRTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQIESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(1835..3250)
                     /locus_tag="IVO69_001950"
     CDS             complement(1835..3250)
                     /locus_tag="IVO69_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7400718.1"
                     /translation="MKITIPENFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAKIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYHSGNYGGR
                     IGCILNPEMVYARSSSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCRKQSIYF
                     DPATEDLETVQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEEQYMDEKGIVQDDYRIEF
                     IGQHLRYLIKAAEEGANCEGYMLRAFTDCVSPMNAFKNRYGLVRIELNDERSRILKKS
                     ASWYQSLIDKRELSLPDEAYK"
     gene            complement(3267..4583)
                     /locus_tag="IVO69_001951"
     CDS             complement(3267..4583)
                     /locus_tag="IVO69_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400719.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLVTGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIVVSILIYLPFVKVANRVENDVI"
     gene            complement(4733..5431)
                     /locus_tag="IVO69_001952"
     CDS             complement(4733..5431)
                     /locus_tag="IVO69_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7400720.1"
                     /translation="MEENKTLVEVTTDKILRYLKEKQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQISRMKSLTQKIETSFREGTQDHVQLDIDFHTTIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMATN
                     RRELQKIIGKKETN"
     gene            complement(5708..6697)
                     /locus_tag="IVO69_001953"
     CDS             complement(5708..6697)
                     /locus_tag="IVO69_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7400721.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
     gene            complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="IVO69_001954"
     CDS             complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="IVO69_001954"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7400722.1"
                     /translation="MDKEQVFIQTLYNLILNPNTRDWERKVLIQTKNDIRENTSAKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLLTEGFDTQQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGNLSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SDLLTIYWQITDPTDAFGQFQDRGKQYRPVIFYQDERQKKLAEQSKQELVSSGTFRQP
                     IVTKIEPVGTFWPAENYHQQFYKKQPKRYKKIQQARKQFLIYQRIKNKW"
     gene            complement(7842..8585)
                     /gene="truA"
                     /locus_tag="IVO69_001955"
     CDS             complement(7842..8585)
                     /gene="truA"
                     /locus_tag="IVO69_001955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7400723.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNSEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHA
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLMGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(8721..9458)
                     /locus_tag="IVO69_001956"
     CDS             complement(8721..9458)
                     /locus_tag="IVO69_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7400724.1"
                     /translation="MFKYMDKIKQLLDLVEKEEKKSIKEAIDVLTQANLQKRSVYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGREVMLDRSPITFTSQMERLEGYGTNLAKTVSF
                     KNQDVLILHSVSGRNPIIIDLALTAKAKGVKIISLTNVQYSRSVTSRHSSGKRLFEVS
                     DIVIDNHGDIGDACCELTGIVQKAGPSSTVIGAAILNTIIVEVCEQLIANGIKHPPIF
                     YSANLDGGDLRNAELYEEYKEVIHYEL"
     gene            complement(9459..10709)
                     /locus_tag="IVO69_001957"
     CDS             complement(9459..10709)
                     /locus_tag="IVO69_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7400725.1"
                     /translation="MNFISEHILKNPPVLLGLIAMLGLFIQRKSFSETVKGALTAAFG
                     MVALTAGVNMLVGTISPINIAVQTQLGVEVTEGLSDVTFTAEYGGTVGLAMFLGLVIH
                     LLIARFTPVKTIFLTGHMLWWFPFVFVAAGVEAGLSGGALIGISGILSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGVLSLVSGFVAKRVGNKEKSTEDIKVPENLSFFREISI
                     TGALVMFLMNTVVGIIAPVLIPEGDNLLMFSIEAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVVLANSFHLFGVLL
                     VPLVITSFFECGGAAVIGEGQGGFRGAVVGTIAASFVMVVLAGFSAIVFSTTIQSWIL
                     IFGGNDLSLWGMIGRGFSGLFGGF"
     gene            complement(10714..10995)
                     /locus_tag="IVO69_001958"
     CDS             complement(10714..10995)
                     /locus_tag="IVO69_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400726.1"
                     /translation="MKILAVCGFGVGSSMVLKMSLDKVIKELGLKATVENTDLSTAKA
                     TMADVYFTSHELLPELKASVKAPVYPIKKYMDTSEIKAQLEQYLADKGE"
     gene            complement(11008..11439)
                     /locus_tag="IVO69_001959"
     CDS             complement(11008..11439)
                     /locus_tag="IVO69_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400727.1"
                     /translation="MSTINKLLPKNQIQVIETAENWMKAVEIASNPLLKNKMITHTYL
                     ENMIRSVEENGPYMVLADYFALMHAKPGLGVNEQSMSLLVVKDAVDMKGKPIHIFLVL
                     AAKNHESHLERLKDIMEIFMDNEKYQTILSGNKETIIQLFA"
     gene            complement(11436..13076)
                     /locus_tag="IVO69_001960"
     CDS             complement(11436..13076)
                     /locus_tag="IVO69_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400728.1"
                     /translation="MSVIQYQESKLRRVAILNRLSNFEHVSYQQLSEEYYVSRSSIAN
                     DISYIKYVFAKEGLNLSFDRFGTYFEGNEIQVQKVLKRMILNHFDELEIVREIVDRKL
                     LAIVERSFRESIADKQIELPESYFSSIIVSILLMIQRSREGKKISLKGKNQYGKYFLE
                     FNKYPLVCELLHQLEEQRIYQFSQEEVQYLTYVIVGSGLRFFMKNETIPLVFREKIRQ
                     LIQKVSRGIQIDLTQDSRLEEDLLVHLYQLLLRIEAQTTIVNPLIDEIKQNYPSLYGV
                     VWFALNDFRWPYQVSLSEDEVGFVAIHFQAAIERVKRLNRLLFVCPNGMGTSSFVSAK
                     IRRILPDIDSIETASVDKLNRLDLFDVDLIISTVDIPKQIRPVVRISPMVTARDMKRI
                     MNHYIDLVIDNEQINIEGSIAEKVKPLISSNVYFDHFDSKEAAIRFLVDQQQFENEES
                     KQRFLYSIFEREEIQSTYLENGFAIPHGNPNVVKQTSVAVLIADKPFSWGYQKADIVV
                     LLMIREEDVKEVEPVMKMVMQGIEDKNWFISKMLEVRE"
     gene            complement(13296..13931)
                     /locus_tag="IVO69_001961"
     CDS             complement(13296..13931)
                     /locus_tag="IVO69_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7400729.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EISSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTASIGFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWSFLNKIGFCQRISFFFNYVPIAWLSILHLRQ"
     gene            complement(14083..>15072)
                     /gene="sstT"
                     /locus_tag="IVO69_001962"
     CDS             complement(14083..>15072)
                     /gene="sstT"
                     /locus_tag="IVO69_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7400730.1"
                     /translation="LSALVAVIISSIFTVPITLQETVSEQAPQNLESVLSTMLTNVTQ
                     NPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISVALSQVVQVIIAFAPIGI
                     LGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLILFCL
                     KESALPAFFTRSSAANIPINMMLAEKLKLTKESYSISIPLGATINMGGAAITISVMSL
                     TAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIV
                     GIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDRLDGLNER"
BASE COUNT         4559 a         3277 c         2458 g         4778 t
ORIGIN      
        1 gacaaagctg tcgaagaaag gtttgttcac gataaatggt gcgctatgtc cagctgcgat
       61 cgcattgaca tttgcttcga ttgccggcgc attgatcgtt tgcatcaaag gatcgaccat
      121 attcgccccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcaataatgc
      181 agatggataa gagttcgcaa gtcccataaa tggttcttgg accactgtat agaacgaacc
      241 tacgatatca tcaaccccaa atgcagcaaa aattgcagca actaaagcaa aggcagagat
      301 agtgatcatt gctggcagta atgaagcaaa tgctttcgca actgctggtg gcacaccatc
      361 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
      421 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
      481 agcaaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
      541 agctaaatga tacgcaagta agaaagcaat caagacagaa agaatggcaa atgtgccgtt
      601 ccaagctgca gccccgaatc ctttccatga ttcaccgcca aaaattgaat tcatcaattc
      661 ttggaatccg ggaatcggta agttattgat cattactgct aatgctccta aaatcattaa
      721 cggcatactc accataaatg aatcccgaat cgcaactaaa tgccgttgtg caccaatttt
      781 tgatgcgaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
      841 ctcttttctt tactgtgaat tacgcgtcca accgacgata cagatcaatg atctcttttg
      901 ctaaatctgt aaaagcaata gatgtcatca ggtgatcttg actgtgaact gtcaaaagtg
      961 ttacttcaac gtggtttcct tgtgcttctt gcgtcagcat ctctgtttga gagtgatgtg
     1021 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatctcctt
     1081 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
     1141 gacccatgat ggcttctaga tttttttgat cttccatttc ttgtttcctc cgatattttt
     1201 attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat
     1261 agtcagccat attgatcaca tctagaggaa tgcctttagg tgctagcttt tcagcaaatt
     1321 gattttgcat aaaacgtact tgcggaccta agagtaaaac gtctacattt ttagattcta
     1381 tttgattatc tgcatctgag gcagagacag caaagatatc tgcttccatt ccttttgctt
     1441 ctgctgcttt ttgcattttt gtgactagga ggcttgtact cattcccgct gaacatacaa
     1501 gcatgattgt tctcttttcc attttaaaca cgttcctttc tattcttaca cttgtatata
     1561 aagcaagtag tgtgccaact ttaaaagcgc tttaaaaagt tgataatatc acgtttatgg
     1621 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattcttt
     1681 tttattgtat ttatttcttt taacgtgcac aaaaaaagtt gtgacaagtc ttttgtcaca
     1741 acttttttga ataagaggtc aaaatgctac tttcacaacc tcctttattc caaataacca
     1801 tcaaatacaa tcaatatctt cgaaaaactt ctagttattt ataggcttca tcaggtaagc
     1861 ttaattctct cttatcaatc agagactgat accaggaagc agactttttc aatattctgc
     1921 tccgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     1981 gtgaaacaca gtcagtgaat gcccgtagca tataaccttc gcaatttgca ccttcttctg
     2041 ctgctttgat caaataacgc aaatgctgtc cgataaattc gatacgatag tcatcttgca
     2101 cgattccttt ctcatccatg tactgttctt catgttctct tcccatacca ttttctgtta
     2161 tcagccaagg gatattatga taattttctt ttagatacat ggctatatcg tacatgattt
     2221 ttggatagat ctcccaccct ctagaattgt tcattttctt tccaggtaaa tcaaagactt
     2281 cataatatcg ttctggatgg aaaggtgtct cttcattcca ttgataacga ggagcacgaa
     2341 cgcgtttagg gtaatattga ttgatcccaa ggaaatctac tgtatgatgc tgaaccgttt
     2401 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt tcgacacaac tctaggaact
     2461 cttctgtgta gtaccctttt accatcggat caaaaaatac tcgattataa aacagatcat
     2521 acatataagc agctttttgg tcttctggag aactggaacg agcatagacc atctcaggat
     2581 ttaaaataca gccaatcctt ccgccataat tgccactatg gtaaatttct acagctttag
     2641 ccgttgctaa tactttgtga taattccaca tcatccattt ttttgtgttt tgttcatgcg
     2701 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
     2761 accagttttt taccctgtca ccgtaacgat caaaagcaat ctttgcgtac ttagcataaa
     2821 aatcaaccac ttttcttgaa gaccagccat catatttttc caaaagaaaa gcagggactt
     2881 cgtagtgttc taaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
     2941 cacttatatg ctgagcatat tcttcatcta ctatacaatt ctcgtaatca gtgaaaaaac
     3001 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc aaatctacat
     3061 cctgttggta ctgctccata aaattagttg ctacggctgg accataccca ttatgccaaa
     3121 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
     3181 tccaaccttc cgtttgccaa gcagaagatg ctgcaccaag aataaaattt tctggaatag
     3241 taattttcat gatttcctcc tgttttttag atcacatcat tttccacacg atttgctact
     3301 tttacgaatg gcagatagat caaaatagaa acaacaatac aaacaagttg cgtaataact
     3361 gcaccgatat ttcctgctgt ggccagataa gcattgacga tcggcggcat cggccaagga
     3421 accattacaa ctgcttttgc cgcaaatcct aaagcggttg caaaataacc aatcgttcct
     3481 gtaacaagcg gcgttaagat aaacgggata gctaaaatag gattcagcat gatcggcata
     3541 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagagataa cttggtgatt
     3601 tgtttcatat cttctcgctt tcccactagc aatactgcta tcagtagacc tatcgtgacc
     3661 ccagacccgc ccatactcat atacatatcc caaaatggca tagtgataat gtttggtact
     3721 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttct
     3781 cgaattggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaatg
     3841 aacatcaata acaaaatacc tggtagccct tgaacgacac cttctaatgg gcgttgtacg
     3901 acattgtaaa tgatatcata aaggtacatc cctgtaagtt ctttgatgat aaaccccgca
     3961 gccgcgatac ctgatactgt cagaatcgtg ggaaataacg cagaaaaact ggcagaaaca
     4021 ttcgttggca cggtatcagg cattctcagc tgtagttttt tctgtttcct caaccagcaa
     4081 taaagttcaa tggaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
     4141 tattgtttag ccaatacgtt ctctactata acagtctgat tttcattcac taattcataa
     4201 acagttggat taacagataa ataagacatc actgcgataa taccagggaa tacaccatcc
     4261 actttattca gctttgctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
     4321 cttagtgtga catagctgat ggcagatgcg ataggtttta attcttctaa aaaacgcaaa
     4381 aaacttatct gtgctaaccc attttccgaa tcaaagacca ttgctgaaaa caacgtgcca
     4441 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
     4501 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
     4561 aaactcattc cagatcccct cattcctgct tcttctcctt gccgtcatct ctttttgcct
     4621 gttagtcagc gcttacattt cggtgaataa aaactatttg cttgtccgtt ttaccaaata
     4681 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
     4741 tttctttctt cccaattatt ttctgtaatt ctctccgatt tgttgccata tggacagtca
     4801 tcgcatcaaa tgccgcaagt acattccgcc tctcgatagc ctgaacgatt tctgtgtgga
     4861 tatcgatcgt ctcgattttc aaatgctgga tatcataatt tttgttaaat aaagcaatcg
     4921 attcattaat gatcggaatg atatgcagta acgctacgtt tccgctagct tctgcaatcg
     4981 tcgtatgaaa atcgatgtct aattgaacat gatcttgtgt accttctcga aaagacgttt
     5041 caatcttttg cgttaagctt ttcattcggc tgatttgatt tttcgttgca tattccgccg
     5101 ctaaccctgc catctttggc tccagtaaat atctcagctc aaacaagtct cgtatcatcc
     5161 ggtcctgatc ttcaatcaaa gaaaaaccga gtggatcatt agcaatccct cgcttatcac
     5221 tgacaaatgt ccccgcccct tgccgaactt caagaatatt cctagaagca agcgctcgga
     5281 cagcttctcg caccgtgctg cgtcccacct ctaaataatc ggacaactca tattcattcg
     5341 gcaatttatc tcctacttgc aactgctttt cctttaaata tctaaggatc ttgtccgtgg
     5401 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
     5461 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat aaaaatcgat
     5521 tcatccgttc atggatatac aaacgaagct atttaccata aaaaaagact aggataaaat
     5581 tcactcctag ccttcgatca tttttatttt gaacgacaaa acgtatgatg ttcaataata
     5641 gatgagcttc attcgattct tgatgacatt ttttgataga tggataaaaa gtttgattac
     5701 atcgtttcta ttctagaatt tggtcgatct tcaattcttc ttctggtgag aaatctaaat
     5761 tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctcca accagtacac
     5821 tcgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgccctc
     5881 gtgatacggc gattgtctgc agtgctttga ttttttccag agttgatcca acttgttctt
     5941 ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaatc ccatgcagat
     6001 aacgatttgt cagcaaacct tgggcaagcg ggctgaacgt gattgctcct agaccttctt
     6061 cttctaatac tggagataag tcgtcttcta gtagttcacg agaaaacata ttgtagcgca
     6121 tctgatgaat aatgaacggt gcttcttttc tttttaaaat ttcagtcatt tttttggtat
     6181 cttctccatt atagttggat ataccaatat ataatgcttt cccttgtctt acaagttgca
     6241 tcaatgcttc tgctgtttct tccaaaggtg tatcagggtc tggacgatga tgatagaaga
     6301 tgtcgacata atctagcccc attctttgga gactctgatc acaacttgaa atgatagatt
     6361 tacgagagcc ccattctccg tatggacctt cccacatatg ataacctgct ttgctagaaa
     6421 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgcccg aagtttttct
     6481 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
     6541 cgaatgcacc tcttaaaatc tctctttggt tatggatagg atcataatca ccaaaatttt
     6601 gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt
     6661 ccattttttc ataacgtgca ctatctgcaa tatacaagtt ttctcctcct taccattttt
     6721 aaaacgcttt ctttcgttct gcttttagtt tactacattt taaagaaaag gagttagtca
     6781 aaatgatttt ttcttgtatt tttttaccat ttgtttttta ttcgttgata gatcagaaat
     6841 tgtttacgtg cttgctgaat ttttttatag cgtttcggtt gttttttgta aaattgctga
     6901 tgataatttt ctgctggcca aaacgtacca actggttcaa tttttgtgac gattggttgg
     6961 cggaacgttc ctgaactgac tagttcctgt ttcgattgct ccgctaattt tttctgacgt
     7021 tcgtcttgat aaaaaatcac cggacggtac tgcttccctc tatcttgaaa ttgtccaaag
     7081 gcatctgtgg gatcagttat ttgccaatag attgtcaaca aatcgctata acttatctct
     7141 ctcgtatcat aaatgatctc cacagcttcc acatggcctg tgtaaccacc acttacttga
     7201 tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacgga taggttccct
     7261 tttttcgatt caaacggctc gaccatgcac cagaaacatc cccctgcaaa tactgcacgt
     7321 tcttgatagt cagcatcttg catagcatgc ttggaaaagt catatttttg ttgcgtgtca
     7381 aatccttccg taagcaataa ataaaagtcc attacgtcag gcgtcagatt cattcggata
     7441 gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttagcgga tgtattctct
     7501 cttatgtcat tctttgtctg gatcaatact tttcgttccc aatctcgtgt attcggattt
     7561 aaaatcagat tataaagtgt ttgaatgaat acttgttcct tatccataga ctctcctcct
     7621 ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc
     7681 gattgaatcg tatctattca aagaagataa gcggcttcag atgatataag atgatcttta
     7741 tcttaaaaag tatactctat ggtttggttt aactaaaaaa cgacctctga aaaaagggat
     7801 gttctccagt tctcagaagt cgagtaaaga tgattctttt tttatgaacg ataataaact
     7861 tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgctgccg cactttgctt
     7921 tcgaatattt catcgattgt ttctatcggc atggcacctg taccgatttc tagtaatgtt
     7981 cccatgatga ttcgcaccat tttatataga aatccgtctc cgacaaaagt aaaatgaagc
     8041 atttctccct tacgttcgat agataattca tcaatcgtac gtaccgtaga tttttttgat
     8101 tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca tcaatttttc gcaagctgca
     8161 ttcagcttat ccatatccaa ctcttgagga aaataaaagc tgtgatttcg ttcaaatgct
     8221 gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt
     8281 gcatggaagc gctcaggcat ctcttcgatt cttttgatga caatatctcg cggaagatag
     8341 cgattcataa agtcgatcat ttctgccagt ccaatttctg agtttgtttt gaaatttgcc
     8401 acttgtcctc gcgcatgcgt gccggcgtct gttcttcctg atccaataat ttctatctct
     8461 tcatttgtca tctgatgcaa cactttttcg attttccctt ggatggtttt atcgtcatct
     8521 ccaagtcttt gccagcctga atatcttttt ccgtcatatt cgatcgtcaa cttgatgttt
     8581 ctcatatttt tgtggctcct ctatattcca tttggtcttc tctctacctt tttaagattg
     8641 tagcacaaat taaagctact tgcttctctg tgagagaaac caagtagctt taaataattg
     8701 tttatgaaca attgtattat ttataattca taatgaatca cttctttata ttcctcatat
     8761 aattctgcgt ttcgcagatc gcccccatct aaattcgccg aataaaatat aggaggatgt
     8821 tttatcccat ttgcaataag ctgttcgcaa acttccacga tgatcgtatt gaggatggct
     8881 gcaccgatta cagtagagga aggaccggct ttttgtacga tacctgtcag ctcacagcaa
     8941 gcatctccta tatccccatg gttatcgata actatatcag aaacctcaaa caatcgtttc
     9001 cctgatgaat gacgactagt gaccgatcgt gaatattgga cattcgtcaa actaatgatt
     9061 tttacgccct tcgcttttgc agttaacgct aaatcaataa tgattgggtt tcttcccgat
     9121 accgagtgaa ggatcaatac atcttgattt ttaaatgaaa ctgtttttgc taaattcgtt
     9181 ccatatcctt ctaaacgttc catttgactg gtaaaggtga taggggaacg atctagcatc
     9241 acttctcgtc caaagatagc attgatggtc atcatcccac ctgcccgata atacatctct
     9301 tccgctaaga taccagcatg actagcacca aaaatataga ccgaacgttt ttgcagattc
     9361 gcttgggtca atacatcgat tgcttctttg attgactttt tttcttcctt ttcaaccaaa
     9421 tctaataact gtttgatttt atccatatat ttgaacatct agaaccctcc aaacaaaccg
     9481 ctaaatcctc taccgatcat tccccataac gacaaatcgt ttccgccgaa aatcaatatc
     9541 cagctttgga tcgtcgtact aaaaacaata gctgaaaaac cagctaatac gaccatgaca
     9601 aatgaagctg cgattgttcc gacaaccgct ccacgaaagc caccttgacc ttcaccgatg
     9661 actgctgcac caccacattc aaagaaactt gtgattacta atggcacaag caacacgccg
     9721 aatagatgga atgaattcgc caaaacaact aaaatcgtag aagtgatcat tgccacgata
     9781 aaaccgataa tcactgcatt tggtcgataa ttgaataaaa taggcacatc aaatgctggt
     9841 ttcgctcccg gtacgacttt ttctgcaatc ccttgaaacg ctggaatgat ttgattgatc
     9901 agtagtcgga caccatatag cataatgagt agtcctgcac caaaattcaa ccctgcttca
     9961 atcgaaaaca tcaggagatt atccccttct ggtatcaaaa caggtgcgat gatcccaact
    10021 acggtattca tcaagaacat gactagtgca cctgtaattg agatttctcg gaaaaatgaa
    10081 agattctcag gtactttgat atcttctgtc gatttttctt tatttcctac tcgttttgct
    10141 acaaatcctg aaactaaaga caaaacaccc gttggatgac caatcaggaa agaatcatct
    10201 cccgtagcat cccagacata tttgcgcatg atccacggca taaaagacca ataacaagcc
    10261 gataaaatcc cgctgatccc aatcaatgct ccccctgaca gtcctgcttc cacaccagct
    10321 gctacaaata cgaaaggaaa ccaccaaagc atatgacctg ttaaaaagat cgtcttcaca
    10381 ggtgtaaacc gagcaatcaa taaatggata actaatccta aaaacatagc taatccgacc
    10441 gttccgccat attctgcagt aaacgtcaca tcagatagtc cttctgtcac ttctacaccc
    10501 aactgtgttt ggacagctat attgattgga gaaatcgttc cgaccagcat attgactccg
    10561 gcggtcaaag caaccatacc aaatgcagcg gtcaaagctc ccttaaccgt ttctgaaaat
    10621 gattttcgct ggatgaacag cccgagcatc gcaatcaaac ctaacaagac gggcggattc
    10681 ttcaagatgt gttcactaat gaaattcatt cttttattcc cccttatcag ctagatattg
    10741 ttccaattgc gcctttattt ctgaagtatc catatacttt ttaataggat aaacaggcgc
    10801 ttttacagaa gcttttagtt ctggaagtaa ttcatgactt gtaaaataaa catctgccat
    10861 cgttgctttg gcagtggata aatctgtatt ttccactgtc gcctttaaac ccagctcctt
    10921 aattacttta tcaagagaca ttttcaaaac catagaactc cctacaccaa atccacatac
    10981 tgctaaaatt ttcatttttc ttcctcctta tgcaaataac tgaatgattg tttctttatt
    11041 tcctgataaa atcgtttgat atttttcatt gtccataaat atttccatga tatcttttaa
    11101 tctctccaga tggctttcgt ggtttttggc agctaaaacc aaaaaaatat ggattggttt
    11161 ccctttcata tctacagcat cttttacgac taaaaggctc atactttgct cgtttactcc
    11221 aagacctggt tttgcgtgca tcagcgcaaa ataatcagcc aataccatat atggtccatt
    11281 ttcctctaca cttctgatca tgttttctaa atacgtatgc gttatcattt tgttttttaa
    11341 taatggattg ctagcgattt cgactgcttt catccaattt tcagccgttt ctattacttg
    11401 gatttgattt ttcggcagca atttattgat agtactcatt ctctgacctc caacattttt
    11461 gaaataaacc aatttttatc ctctattcct tgcatgacca ttttcatcac aggttcgact
    11521 tctttcacat cttcttctcg gatcattagc agaacaacga tatctgcctt ttgatatccc
    11581 catgaaaatg gcttgtctgc tatcaataca gcaaccgaag tttgtttaac gacattggga
    11641 ttgccatgag gaatagcaaa accattctcc aagtatgttg attggatttc ctcacgctca
    11701 aaaatagagt agagaaatcg ctgtttcgat tcttcattct caaactgttg ctgatcaacc
    11761 aaaaaacgga tagcagcttc ttttgaatca aagtgatcaa aatagacatt agaggaaata
    11821 agaggtttca ctttttcagc tatcgagcct tcgatgttta tttgttcgtt atctatgacc
    11881 agatcgatat aatgattcat gatacgtttc atatctctag cagtaaccat tggcgaaatt
    11941 cttactactg gtctaatttg tttaggtata tcgacagtgg agataatcaa atctacgtca
    12001 aacaaatcta gtctgttcaa tttatcgaca gatgctgttt cgatactatc gatatcaggt
    12061 aagattcttc tgatttttgc agaaacaaat gaactggttc ccattccatt gggacatacg
    12121 aataagagtc tatttaatcg tttgacacgc tcgattgctg cttggaaatg gatagctaca
    12181 aaacctactt catcttcgga caagcttact tgataaggcc agcgaaaatc atttaacgca
    12241 aaccagacaa caccatacaa agaaggataa ttttgtttta tctcatctat caaaggattt
    12301 acgatggtcg tttgagcctc aatacgtaaa agcagctgat acagatggac aagcagatct
    12361 tcttctaaac gactatcttg ggtaagatct atctgaatgc ctctactcac tttttggatc
    12421 aattgccgga ttttttctcg aaatactaaa gggatcgttt catttttcat gaaaaagcgt
    12481 aaaccacttc ccacaataac ataagtcaaa tactgtactt cttcttgcga aaattggtaa
    12541 atacgctgtt cctctaactg atgcagcaat tcacaaacga ggggatattt gttgaattct
    12601 aaaaaatact ttccatattg atttttacct tttaaactga ttttcttccc ttctcttgaa
    12661 cgctgaatca tcaataagat tgaaacgatg atacttgaaa agtagctttc tggtagctca
    12721 atttgcttgt ctgcaatgct ctcgcgaaaa gatcgttcga ctattgctag aagctttcga
    12781 tccactattt ctcttacgat ctccaattca tcaaaatgat tcaggatcat acgcttcagt
    12841 accttctgaa cctgtatttc atttccttca aaatatgtcc cgaaacgatc aaaactgaga
    12901 ttcaaccctt ctttcgcaaa tacatatttg atgtaagaaa tatcatttgc gatactgctt
    12961 ctggaaacat aatattcttc agaaagttgc tggtaactta catgctcgaa attggacaat
    13021 ctgttcagga tcgctactct tcgtagtttg ctttcttgat attgtattac actcatttcc
    13081 atcaccttct atattcacta taattttttc ttataaggag aactagacca aataaaagat
    13141 taaaataacc tgagaaatca tacactgctc tttctcagtt aatatacgct aaaacagaag
    13201 ctatacaaga aaattatgga actatatttc agaaaaacaa aaaaacagcc aacggacgaa
    13261 atgtccgctg actgttcaat ttgatggctc aagttttact gtctgagatg caagatactc
    13321 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa tcctatttta
    13381 ttcaggaatg accaagaatt tatagaagga attcttgcca gaccataaat cgccgcaaaa
    13441 agcaaactga tcacagacaa tccaaggtat aagtggctta cgtccatttg cccatttttt
    13501 cgatagatca agaaaagaag aactggaaac aataaaaaac cactataacc taaacctgat
    13561 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
    13621 aaacctgtaa agatacaatc acctatacca taaaaaccga tagatgctgt aaccagggta
    13681 gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa
    13741 aaaatccctg aaaccactga ccaaataaga aatgcttttc ttacaggact ggaaatctct
    13801 ccaaatacac taatcacact cttcatttga tctaattcag gataatacaa tcctaatacg
    13861 tacggagtca aaaaatcact caacacgcct aataataaaa agaagaaacc aaatttacgt
    13921 aaaaaatcca tgatgcgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
    13981 gactttatct cgatttaaca ggatcctcta ttttacacga acatctacat aactcattaa
    14041 aaatttgaat cgtcttaagg ttcatcccta aatatctaat attcaacgtt catttaaccc
    14101 atctaatcga tctttcaatt tgattttttc tccacttctt ttacgcgcgc ctaattctcc
    14161 tattgcagtg aacaacagat cacttgagga atttaaggca gtttccattg aatcttggat
    14221 aacaccgata ataaatccga taccgacgat ttgcatcgca acatcattag agataccaaa
    14281 caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagccccaca
    14341 agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaatggtg cttcaatccc
    14401 taaggaatgt acagccgtca atgacatcac ggaaatggta atcgcagcac cgcccatatt
    14461 gatcgtagca cctaatggga tcgatatgga ataagactct ttcgtcaatt tcaatttttc
    14521 agccagcatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag
    14581 cgcactttct ttcaaacaaa aaagaatcaa tggatagggg ttttctttaa taaacaaaaa
    14641 cgtgaggaac gggtacacaa ctaatgcgac aaacagcatc gtgccaatca acacaagtaa
    14701 aagctgcaga tactctgcca atccagcgat tccagtcgtg gcaatcgatt gataaaccaa
    14761 tcctaaaatc cctatcggag cgaatgcaat aatcacttgt acgacttgtg ataatgctac
    14821 agatatttga tccaccaatt cttttgtcga ttctgaacgt gctcgaaaag ccagacctaa
    14881 caacacagcc cagaataaga caccaagata attcgcatca atgagcgatt gaacaggatt
    14941 ttgtgtgaca tttgtcaaca tcgtagataa aacactttcc aaattttgcg gtgcttgttc
    15001 agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttataa tcactgctac
    15061 aagtgccgaa ag
//
LOCUS       DACCPY010000071        14987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCPY010000071 DACCPY010000000
VERSION     DACCPY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.073"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(369..995)
                     /locus_tag="IVO69_001963"
     CDS             complement(369..995)
                     /locus_tag="IVO69_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400731.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="IVO69_001964"
     CDS             complement(1290..1625)
                     /locus_tag="IVO69_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7400732.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="IVO69_001965"
     CDS             complement(1612..1896)
                     /locus_tag="IVO69_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7400733.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="IVO69_001966"
     CDS             complement(1952..3475)
                     /locus_tag="IVO69_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7400734.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="IVO69_001967"
     CDS             complement(3468..4643)
                     /locus_tag="IVO69_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7400735.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="IVO69_001968"
     CDS             complement(4647..4832)
                     /locus_tag="IVO69_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400736.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="IVO69_001969"
     CDS             complement(4798..6492)
                     /locus_tag="IVO69_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7400737.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="IVO69_001970"
     CDS             complement(6489..6962)
                     /locus_tag="IVO69_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7400738.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="IVO69_001971"
     CDS             complement(7031..7186)
                     /locus_tag="IVO69_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP7400739.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="IVO69_001972"
     CDS             complement(7320..7700)
                     /locus_tag="IVO69_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7400740.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="IVO69_001973"
     CDS             complement(7704..7919)
                     /locus_tag="IVO69_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400741.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="IVO69_001974"
     CDS             complement(7922..8329)
                     /locus_tag="IVO69_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7400742.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="IVO69_001975"
     CDS             complement(8595..10070)
                     /locus_tag="IVO69_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7400743.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="IVO69_001976"
     CDS             complement(10060..10908)
                     /locus_tag="IVO69_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7400744.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="IVO69_001977"
     CDS             complement(10945..11307)
                     /locus_tag="IVO69_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400745.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="IVO69_001978"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="IVO69_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="IVO69_001979"
     CDS             complement(12228..12539)
                     /locus_tag="IVO69_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400746.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="IVO69_001980"
     CDS             complement(12583..12876)
                     /locus_tag="IVO69_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400747.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="IVO69_001981"
     CDS             13070..13717
                     /locus_tag="IVO69_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400748.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="IVO69_001982"
     CDS             13778..14923
                     /locus_tag="IVO69_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7400749.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4323 a         3079 c         2390 g         5195 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggttt
//
LOCUS       DACCPY010000072        14924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCPY010000072 DACCPY010000000
VERSION     DACCPY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.074"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            12..851
                     /locus_tag="IVO69_001983"
     CDS             12..851
                     /locus_tag="IVO69_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7400750.1"
                     /translation="MDKPLYFKVDKKIKKLASTQQLQSRKSKLLFLALVFEDQSYVII
                     DQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKEL
                     ANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTAD
                     SLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIRE
                     GKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTV
                     SLP"
     gene            931..1905
                     /locus_tag="IVO69_001984"
     CDS             931..1905
                     /locus_tag="IVO69_001984"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7400751.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2094..3230
                     /locus_tag="IVO69_001985"
     CDS             2094..3230
                     /locus_tag="IVO69_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7400752.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3367..3711
                     /locus_tag="IVO69_001986"
     CDS             3367..3711
                     /locus_tag="IVO69_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7400753.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3982..5268
                     /gene="efmA"
                     /locus_tag="IVO69_001987"
     CDS             3982..5268
                     /gene="efmA"
                     /locus_tag="IVO69_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7400754.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5406..6005)
                     /locus_tag="IVO69_001988"
     CDS             complement(5406..6005)
                     /locus_tag="IVO69_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7400755.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6409..7530
                     /gene="mnmA"
                     /locus_tag="IVO69_001989"
     CDS             6409..7530
                     /gene="mnmA"
                     /locus_tag="IVO69_001989"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7400756.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7776..9197
                     /gene="arcD"
                     /locus_tag="IVO69_001990"
     CDS             7776..9197
                     /gene="arcD"
                     /locus_tag="IVO69_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7400757.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9211..10329
                     /locus_tag="IVO69_001991"
     CDS             9211..10329
                     /locus_tag="IVO69_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7400758.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10389..11681)
                     /locus_tag="IVO69_001992"
     CDS             complement(10389..11681)
                     /locus_tag="IVO69_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7400759.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11831..12391
                     /locus_tag="IVO69_001993"
     CDS             11831..12391
                     /locus_tag="IVO69_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7400760.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12520..12897
                     /locus_tag="IVO69_001994"
     CDS             12520..12897
                     /locus_tag="IVO69_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7400761.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12830..14902
                     /locus_tag="IVO69_001995"
     CDS             12830..14902
                     /locus_tag="IVO69_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7400762.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4874 a         2657 c         3237 g         4156 t
ORIGIN      
        1 tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag
       61 ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg
      121 aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa
      181 aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa
      241 tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc gaagaaaaaa
      301 ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt
      361 ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc
      421 ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt cgcaagaaaa
      481 atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac
      541 ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca
      601 atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac
      661 gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc
      721 attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac
      781 acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt
      841 cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc
      901 gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg
      961 aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga
     1021 acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc
     1081 gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat
     1141 gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg
     1201 atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat
     1261 gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta
     1321 ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat
     1381 ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac
     1441 atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat
     1501 ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat
     1561 gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga
     1621 gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc
     1681 tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc
     1741 aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta
     1801 acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca tgaaaataca
     1861 ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg
     1921 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
     1981 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
     2041 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
     2101 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
     2161 cggcaatcat gcaagatacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
     2221 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
     2281 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
     2341 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
     2401 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
     2461 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
     2521 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
     2581 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
     2641 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
     2701 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
     2761 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
     2821 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
     2881 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     2941 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     3001 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     3061 gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca atgtatggag
     3121 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     3181 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     3241 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     3301 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     3361 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     3421 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     3481 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     3541 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     3601 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     3661 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     3721 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     3781 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     3841 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     3901 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     3961 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     4021 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     4081 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     4141 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     4201 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     4261 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     4321 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     4381 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     4441 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     4501 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     4561 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     4621 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     4681 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     4741 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     4801 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     4861 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     4921 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     4981 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     5041 cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg attattattt
     5101 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     5161 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     5221 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     5281 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     5341 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     5401 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     5461 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     5521 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     5581 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     5641 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     5701 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     5761 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     5821 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     5881 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     5941 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     6001 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     6061 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     6121 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     6181 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     6241 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     6301 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     6361 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     6421 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     6481 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     6541 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     6601 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     6661 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     6721 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     6781 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     6841 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     6901 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     6961 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     7021 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     7081 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     7141 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     7201 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     7261 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     7321 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     7381 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     7441 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     7501 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     7561 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     7621 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     7681 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     7741 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     7801 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     7861 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     7921 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     7981 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     8041 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     8101 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     8161 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     8221 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     8281 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     8341 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     8401 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     8461 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     8521 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     8581 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     8641 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     8701 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     8761 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     8821 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     8881 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     8941 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     9001 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     9061 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     9121 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     9181 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     9241 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     9301 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     9361 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     9421 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     9481 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     9541 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     9601 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     9661 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     9721 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     9781 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     9841 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     9901 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     9961 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
    10021 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
    10081 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
    10141 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
    10201 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
    10261 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
    10321 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
    10381 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
    10441 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
    10501 ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa tgcactggaa
    10561 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
    10621 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
    10681 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
    10741 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
    10801 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
    10861 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
    10921 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
    10981 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
    11041 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
    11101 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
    11161 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
    11221 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
    11281 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
    11341 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
    11401 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
    11461 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
    11521 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
    11581 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
    11641 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
    11701 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
    11761 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
    11821 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
    11881 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    11941 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    12001 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    12061 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    12121 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    12181 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    12241 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    12301 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    12361 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    12421 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    12481 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    12541 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    12601 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    12661 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    12721 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    12781 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    12841 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    12901 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    12961 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    13021 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    13081 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    13141 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    13201 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    13261 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    13321 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    13381 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    13441 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    13501 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    13561 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    13621 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    13681 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    13741 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    13801 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    13861 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    13921 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    13981 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    14041 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    14101 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    14161 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    14221 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    14281 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    14341 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    14401 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    14461 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    14521 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    14581 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    14641 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    14701 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    14761 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    14821 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    14881 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtga
//
LOCUS       DACCPY010000073        14409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCPY010000073 DACCPY010000000
VERSION     DACCPY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.075"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..655
                     /gene="uxuA"
                     /locus_tag="IVO69_001996"
     CDS             <1..655
                     /gene="uxuA"
                     /locus_tag="IVO69_001996"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7400763.1"
                     /translation="QLIHSQSKGFKLPGWEEERLKKFAQLEKLYEGMTEEELFENLTY
                     FLEKVIPVCEKADVKMAIHPDDPPWELFGFPRVTKNLGDLKKILNAVDSPYNGLTLCT
                     GSLGANPENDLVEIIHEVGSRIHFVHFRNVEFLGERKFKETAHPSTEGSLDMYHIMKA
                     LVQEGFDGPIRPDHGRTVWGEVAMPGYGLYDRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            648..1361
                     /locus_tag="IVO69_001997"
     CDS             648..1361
                     /locus_tag="IVO69_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7400764.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            2113..2313
                     /locus_tag="IVO69_001998"
     CDS             2113..2313
                     /locus_tag="IVO69_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7400765.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            3009..3443
                     /locus_tag="IVO69_001999"
     CDS             3009..3443
                     /locus_tag="IVO69_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400766.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRKCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(3504..3869)
                     /locus_tag="IVO69_002000"
     CDS             complement(3504..3869)
                     /locus_tag="IVO69_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400767.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3945..4190)
                     /locus_tag="IVO69_002001"
     CDS             complement(3945..4190)
                     /locus_tag="IVO69_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400768.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(4260..4514)
                     /locus_tag="IVO69_002002"
     CDS             complement(4260..4514)
                     /locus_tag="IVO69_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400769.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(4678..5001)
                     /locus_tag="IVO69_002003"
     CDS             complement(4678..5001)
                     /locus_tag="IVO69_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400770.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            5188..5261
                     /locus_tag="IVO69_002004"
     tRNA            5188..5261
                     /locus_tag="IVO69_002004"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5222..5224,aa:Ile,seq:cat)
     gene            complement(5646..6143)
                     /locus_tag="IVO69_002005"
     CDS             complement(5646..6143)
                     /locus_tag="IVO69_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7400771.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(6183..6992)
                     /locus_tag="IVO69_002006"
     CDS             complement(6183..6992)
                     /locus_tag="IVO69_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7400772.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            7326..8183
                     /locus_tag="IVO69_002007"
     CDS             7326..8183
                     /locus_tag="IVO69_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7400773.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8176..9078
                     /locus_tag="IVO69_002008"
     CDS             8176..9078
                     /locus_tag="IVO69_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7400774.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            9111..9461
                     /locus_tag="IVO69_002009"
     CDS             9111..9461
                     /locus_tag="IVO69_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7400775.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            9495..10433
                     /locus_tag="IVO69_002010"
     CDS             9495..10433
                     /locus_tag="IVO69_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7400776.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            10511..11404
                     /locus_tag="IVO69_002011"
     CDS             10511..11404
                     /locus_tag="IVO69_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400777.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            11442..12005
                     /gene="amaP"
                     /locus_tag="IVO69_002012"
     CDS             11442..12005
                     /gene="amaP"
                     /locus_tag="IVO69_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7400778.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            12019..12207
                     /locus_tag="IVO69_002013"
     CDS             12019..12207
                     /locus_tag="IVO69_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7400779.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            12220..13122
                     /locus_tag="IVO69_002014"
     CDS             12220..13122
                     /locus_tag="IVO69_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7400780.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            13154..13396
                     /locus_tag="IVO69_002015"
     CDS             13154..13396
                     /locus_tag="IVO69_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7400781.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(13652..14023)
                     /locus_tag="IVO69_002016"
     CDS             complement(13652..14023)
                     /locus_tag="IVO69_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400782.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         5104 a         2388 c         2766 g         4151 t
ORIGIN      
        1 tcaattgata cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt
       61 gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga
      121 aaatctgact tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat
      181 ggctatccat ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa
      241 tcttggcgac ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct
      301 gtgcacagga tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt
      361 aggttcgcgc atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt
      421 caaagaaacg gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc
      481 gttggtgcaa gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg
      541 tgaagtggct atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca
      601 aggtctatat gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca
      661 agctcttcat ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg
      721 cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac
      781 taactggcca aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg
      841 ttccatatat caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag
      901 ataagagagt atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag
      961 gaatccatac aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa
     1021 tggaaaatct atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa
     1081 agccggaacc agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag
     1141 cacttgtatt tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt
     1201 cttttctcat caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga
     1261 aagtagatgt aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc
     1321 aagcaaaacg atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta
     1381 gtcctcttta tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt
     1441 attttgaagt atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta
     1501 ctgttatcta ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt
     1561 ccactcctta tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg
     1621 ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg
     1681 ttactcgata ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata
     1741 cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat
     1801 tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa
     1861 caagaaaaca aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa
     1921 tagaagacaa tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga
     1981 tatattaaat gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa
     2041 actccttttg cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg
     2101 aggaatttat ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc
     2161 tttatcactg gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac
     2221 ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt
     2281 ggccctcaag caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc
     2341 gaaagaaaag acggtttttt tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag
     2401 tagtgtataa aacaattttg tggagcgtga agcttttatg gtagaggaaa atacgaataa
     2461 agtgatgttg ataaaaagat tcctatcttg aatatgaatc gagatgtctt gaaaatgaat
     2521 gagtttgacc aatcattatc ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt
     2581 aatgaaaaag taaaaaagaa tatgttctag gaagagggcg gcacatctaa atttttaagt
     2641 aacggacagt tcaaatatca agcattgaaa gaggctatcc tactgtttga aggaatacga
     2701 atatataaaa acgacaaaga ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga
     2761 atgagatcta ataaaacctg atctttcatc tgttttttta ataacgtaat aattttttta
     2821 ttttttgtgc tttcgccgtt attgtagagt gatttagcat tcttattttt aaaaataact
     2881 gtgttatata ttaaaaaagg cataatagtt ataagctttt ttaaagaata tttagaaatt
     2941 attaattttc gtctgaaaac aaaaaaagaa agcgttatta tttagtctat tttcaggggg
     3001 ggaaccttat gataaaaaaa ctatttttac tatcaatatt aacgctagca atggcaggtt
     3061 gttctgactc tggcttagca actgaactga tcacaaaatc aactgaagcc acgacagagg
     3121 agttaaaatg tggaaacgat cacagtgact acagaggctt aaatcgacga tgcatgggaa
     3181 tattaggcag aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt agaagaagca
     3241 acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg ctgtgcatcg tatacagacg
     3301 tcgaagatgc ggtactttta aaccacgtca ggaaatgttc atttatgtct agggaagttg
     3361 gcgatgaacc aaaactagag tacgctttta gttttgcacg tgaggatgaa gataacccta
     3421 aaacttacga cattttagct tgatctaaga tggttaaagc tttttaatga agtaagagga
     3481 gatagaaggc ttttctagtt atattaggta agataaattc ctttttgctc caaatttgct
     3541 aaacattctg ctagataaac ttcatcgtgt tccggataaa tcggcacgtt gcgctctaca
     3601 gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat acataggatt tttccactct
     3661 ggtgctgggc tgtaaccacg tttttccatt tcatccatga caagctgatg atattggaat
     3721 aatctataag gagaatagtg aaaaacataa tccacagtag catgtttctt tccccagcca
     3781 tttcctctta atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg aggaagcttt
     3841 gaaatcaatt tttcatgcca taatcgcata gaaatgtctc ctttatatat agattgttaa
     3901 ccttagacgt taaatgaaaa aaccagcacc aaggctggaa aaaatcactt cagtttgctt
     3961 gctgtttctg agcgaccaat taggtaatca acggaaatat cgtaaaagtc agcgatatag
     4021 attaattcaa tgatgcttgg aatttcttct ccttcttcgt acttacgata tttgaataaa
     4081 ggaatactta acgcgctaga aacagctact tgagataaag atcggccagt tcttaagaaa
     4141 ttcagacgac aacgtaaagt atccagtaaa atagcttctt cgatactcat ttaatcacct
     4201 ttcattatag tagaatagtt ataatgacta tagaaagcta cacatgcaat caatagtact
     4261 caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga caaaaccagt
     4321 cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt aaaaactttc
     4381 cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc tttgttccaa
     4441 ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa caaaactctc
     4501 accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat aacatgttat
     4561 tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa tgaaaaatga
     4621 aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt gtcttatcta
     4681 tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa agaggacgat
     4741 cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat atttaacaaa
     4801 tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca tgacaagaag
     4861 caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg aggtttgata
     4921 atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg caataaatgg
     4981 caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt ctaaaataag
     5041 caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata agaataacaa
     5101 tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta tagttgaaaa
     5161 gtaacaaaaa taaattttat ttatgatgta cctttagctc agttggttag agcagacggc
     5221 tcataaccgt ccggtcgtag gttcgagtcc tacaaggtac ataagaggtt gtgaaaaagc
     5281 tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttacttgg
     5341 ctgttcttat tactgcagat gcaagctttt gaataccgtt taaggaagag gttgtgaaat
     5401 cagcgttttg acctgagtat tagcttaaca aacggaaaaa tagttcctca tattttgccg
     5461 tttgttaagc taataccgag ggtcagctga ttgaacacca tttaagaaag aggtcgtgag
     5521 cctgccgttt cactctgtgt cataaaaaag ccaatctact ttgatagaaa aagcacgtag
     5581 gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa atatcgtaaa tgaagataga
     5641 tatttttatg attcgacgat agagacgttg aaatctttca acagctctaa agcggattct
     5701 ccgtgttttc tactggagac taacattgga tcaacaaaaa agtctgcttc aggaaaagca
     5761 ctctgcaggc ctatcagatt agagagtaca cacaagtttg tttcaatccc gactacttcg
     5821 atctcttttt gttcttcttt gctttcaaat aaagtttcct ggatttcagc taaagtttca
     5881 ggtggaatcg taaagtaata ctttttaatc actcgttcat tttcttgtgg agaaagaaga
     5941 gggatcaatt gcaaatcttc tcgttcttct tctttgtcct tcagttcaac tggtatatcc
     6001 cgtgtgaaga gaatatattc attattttgg cgtgctttag ctaatctttg agcaatacgt
     6061 tctgccaatt gatctgactc tggaatataa aattcgctgt ttggatcaag aatatgattt
     6121 tgcatatcaa taataactaa cattttcttt cctcctacat tcatatcgtc attttattct
     6181 ttttaataga agttttttaa aatataagca acaccagctt cttcattcgt cagtgttact
     6241 tggtttgcaa tttcttttac ttgtcgctgt gcattcccca tcgcaatact caacccgctg
     6301 taattcaaca tagcaatgtc gttttcttga tcgccaaaag ccatgatgtc actcgattcg
     6361 attccaaggt aatccgccag aaaaacaagt gaatgagctt tagtgatacc tgtaggcgtg
     6421 atttctaaga aatgcggatc agagaatact gattggatac catctgcttg cagatctctt
     6481 tgtagcttct gtagtttttc ttcactctta tctgttaatg ctaatttaag atagctaaga
     6541 gaaggatctt ccaacatatt taaaatttga tcaaaagaat ggttctgtaa attgaaatgt
     6601 tcataataag ggtcatcttt gactagattt gattcatcga atgtcagctg atcatcttgg
     6661 gtttctcggc tagcaatgat ggatattttc gcttcttgag catagaagaa ggcttttctc
     6721 agtgtatcgc tatctatttt tgaacgtctg agaactttcg ccatttttcc atccttcatt
     6781 tcagcaatca gtgaaccatt ggaagaaatc aaatagccac cgattcctaa ttcggaaaaa
     6841 atatctttag cagaaatgct gttccttccg gtagcgacgg ttacgattcc gccattttca
     6901 tcaaatttcc gaagtgcttg gatattttct tctggtatct ttccatttcg atcaagcaag
     6961 gtaccgtcta aatcaatcgc tgctagtttc attttatctt cctactttct ttttacatat
     7021 ctattttcct gttaccttaa gattaaatga gaatttaaaa aaatgaaact aacttgcttt
     7081 gtcattatga attttttcaa aaaacattct taagaaaaag gtggctatat atagtagttt
     7141 ttggtcaata cctttgaata aaaagccatg aaaaacgaat agttagcgct taccgatgcg
     7201 ttttgtgata aaaaaatttt aaaatattca atgaaaacaa agcatatatt ttgctttaaa
     7261 accaaatgag gaatattcta gatgtagaac aggttagaaa aagattagaa aaaggagtga
     7321 ttttgatgga gaagagatac ggcgtagttc ctgtggtaac tgcttcagac gaaaattatg
     7381 ctccttactt gagcgtgatg atcgcaactg cattagaaaa ttgcaataaa acaagacgca
     7441 taaaatttta tgtgattgac gatggtctat cagaatatag taaagaagga ttggaagaaa
     7501 cggtaaataa atattcaagt aatgcaagta tccaattcct gacagtggaa aaagatatct
     7561 acgaagactt cctagtcagt gaccatatca caactaccgc ttacttaaga atttctttac
     7621 caaacttatt agctaaagag gattataaga aagtccttta cttagattca gatgtgttag
     7681 tactagacga tatcgtaaaa ctatatgatg agccgttaaa tggaaaaaca atcggcgcta
     7741 ttatcgatcc aggccaagtg aaagctttgg aaagattagg aatcgattcc gatgacttgt
     7801 acttcaattc aggagtgatg gtgattgaca ttgatcaatg gaataaaaaa gaaattacag
     7861 aaaaaactat ccattacctc agtgaaaatg gcgatcgaat catctatcac gaccaagatg
     7921 ctttgaatgc tgtactttat gaagattggg aacaacttca tccaaaatgg aacatgcaga
     7981 cttcgctgat ttttgaacgt catccagcac caaatgaaaa atatgaacgt cagtataaag
     8041 aaggaaatga aaaaccttcg atcgttcact ttacaggaca tgataaacct tggaatacgt
     8101 tgaaagatca tccgtatacc aatttatact taaaaaaatt agcacacagt actctaacga
     8161 aagtaggcga gatcaatgaa taaaaaagaa atagcagtag tcgcaagttg taacacaaaa
     8221 ttcgtaccac atttagctgc attatttgtc tcagtattag ataattgcaa cccgtctaaa
     8281 tttgttcggt tctatgtgat tgacgatgat atcgattttg aaagtaaaca gcttttacgc
     8341 ttctcagtga aaaacgcacg aatgaactca gatgtggaat tcttgaaaat caataaagaa
     8401 ttctttacaa atgtggtaat cagcgatcgt attccagaaa cagcttatta tcgtattgcg
     8461 attccggaat tgttccgcgg cacagaagtt gaacgtatct tatatatgga ttgcgacatg
     8521 atcgctttac aagatatctc taaattatgg cgtcttgact tcggtgattc aatcgtagca
     8581 gctgttgaag acgctgggtt ccatcaacga ctggaaaaaa tgaaaatttc agctaaatca
     8641 atgcgttatt tcaactcagg tttgatgctg atcaatgtga aaaaatggtt agatgaaaat
     8701 attacgcaaa aagtattaga tttcatcgaa cataatccag aaaaattacg attccatgac
     8761 caagatgcat taaatgctat cttacatgac cgttggttac cacttcatcc gcgttggaat
     8821 gcacaaggct atatcatggc aaaagcaaaa aaacatccaa cagcagcagg tgaacgtgaa
     8881 tacgaagaaa ctcgcaacaa tccttacatt atccattttt caggacatgt gaaaccgtgg
     8941 agcaaggatt ttgaagggcc aacgaagaaa tattacgaaa aatatgcagg gatgacggct
     9001 ttccgttgtg tagctaaatt ccctaaatat ccaaaatatg caaaagttca accaaaaaca
     9061 gatgtagttg ttaaataata gaagacaaca actgaagagg gggagtagga atggaaatta
     9121 caatgtctaa aaatgcagta gagacgttaa ttgaaaaggt tggggaaaat acaaaaatcg
     9181 cactcgcgct tatcaatgat tccgatccat ttctaaggga caaaggtgct tttgcaaaag
     9241 gcagcttttt tcaaatcatt ccttttgttt cggaatttgg agaatatgcg actaaaatcg
     9301 agcatccatt attggacatc tatacatcta aattagagca aaattatttc ggtaaacgat
     9361 tgaacatgga cttcaacaaa cagcttgaca gcttttcatt agaaaatgaa atcgcagtac
     9421 tcgattataa tatcaaatta aagaactgct tcttcagcta atagtttcga gaaagtccaa
     9481 aaaggaggta tacattgaga tactactttg tggatgaaga aaagaatgta atagaaacag
     9541 atgaaaaaaa atataattgg ctcgtacttg atagtgcaaa gcccgaagag attgagcaag
     9601 caatcgaaga attcgagctt cctgatgata ttttcgtagg aaccgaatat ccggaagaag
     9661 tttctcggat ggagcgcctt tatgagacga aactagagca tccattttct ttggtagtga
     9721 ttaatctaga tcagtcagat aatcgtatcg aaaaaaaatt aacgccaata tcttttgtac
     9781 tttccgataa tctcttgatt acttgtatcg atagaaaaac cgaatttgtg gatcggctgc
     9841 tgacaaagca tggaaatcat ttaaactcgt ttgaaaagat catcacgtat acattattag
     9901 atatttacac ccattacgtg aaagaactaa gagagatgaa aaagcgaatt gacgctttgg
     9961 ataaagcagc tcgtaaaaca acggaaaatg aagaactgta taaacaggca gatcttgaaa
    10021 gagacatcgt atatatcgat cataccttaa gagatcaacg ggaaacaatg gatcaattat
    10081 ggaattctga aactttcatc aaacgtttag acgatgaaca gctactctat gatgtccatt
    10141 tgagacagcg acaaacagaa aaaatgatcg agatctatcg agatttatta gagagtattg
    10201 gtgatctatt caacgggatg atggacaata atctaaatca tatcatgaaa tatctagatt
    10261 cagctgcatt aatcatttcc gttccggcgt tgttcgcagg gatttgggga atgaatacag
    10321 gaggattacc gggtaaaagt tctgctctag gctttatact cgtagtcttt ggctctttga
    10381 tagcagcagt agttttaggt taccatttat accggaaaga ttatacaaat taaagaatct
    10441 atttaaaaca ggtatcaaac aaagaaattt gttttggcaa tacaatttaa aaactaggag
    10501 gaatacagca atgacagaac caaaattaat cgacccgcga aacttgtacc atacggagaa
    10561 atttcctaaa caagatcagg atacaccagc tttacagcag aatatgattc ctgttcctga
    10621 ttgtggagaa gaatcctatg aaggcaataa tcaactagaa aaccgccgtg tactaatcac
    10681 tggtggagat tcagggatcg gtcgtgcagc agcgattgca tttgctagag aaggggcaga
    10741 tatcgctctt caattcttcc caggagaaga aaaagatgca gaggaagtag caaaatacgt
    10801 gaaagatgca ggacgtaaga ttgtcttact accttttgat cttcgtgacg aacaagcacc
    10861 taaagaaatc atagataaag ccgtgagtga acttggcggg ctagatacga tggtactaaa
    10921 cgcaggacag caaatttctt gtcagtctat tgctgagtta ccacttgagc aagtcaaaga
    10981 cacatttatg ataaacatca tcgctatgtt tgctttagtg aaagaagctg ttccgcatat
    11041 cccagcagga ggagcaatcg taacaacaac ttctgttcaa gcattcaatc caagcgaaca
    11101 tttattagat tacgcagcaa caaaagcttc aatcgctaac tttacgatcg gtttagcaaa
    11161 acagcttgca cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa tctggacacc
    11221 attacaacta gacgaaggac aaccttctga ttcgatccct gaatttggtc agcaaagttt
    11281 actagaacgt gcaggtcagc caggagaatt agcaccagtt tatgttttct tagcatcgaa
    11341 taaagcaagt tatgttacag cccaaattta cggagtaact ggtggagaag cgatcaactt
    11401 ataagaagtt gatcgtttca cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa
    11461 cgatcggagt gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa
    11521 gtgcaaccta tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt
    11581 catacgtgca acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca
    11641 cgttacttgt tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg
    11701 atggggagtt gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg
    11761 aagatgaatt catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg
    11821 tcaatgtaaa aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga
    11881 tgaaagaaat cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat
    11941 cggtgaaata cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag
    12001 aatagggggg gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt
    12061 ggggtacttg gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta
    12121 ttgatcttga tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa
    12181 atcttagatc actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat
    12241 aataacaata aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca
    12301 aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca
    12361 gatccagtag tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag
    12421 catgcacctc atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc
    12481 gatccttcta aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa
    12541 aaacttagtt ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac
    12601 caaggaaagc atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat
    12661 aaagttgtgc aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca
    12721 aacggtggtt tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct
    12781 gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa
    12841 tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta
    12901 aaaaatatga cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca
    12961 aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa
    13021 aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc
    13081 gcacaaaaag taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta
    13141 ggaggaacga aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat
    13201 tgcaggagca atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg
    13261 tttagttgga tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg
    13321 aatggcaatc gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt
    13381 tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa
    13441 gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta
    13501 tcgaagatgc aagcttttga acactgttta tacggaataa gaggctgtga caagactttt
    13561 gtcacagcct cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta
    13621 aatgtgtctt tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc
    13681 ttgagatagg aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc
    13741 acaaatggat cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat
    13801 tgaaaaagac aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat
    13861 agctcgtaaa gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa
    13921 tcgtatcatg ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat
    13981 agatcacgag taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg
    14041 tgtgagttcg ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta
    14101 acaaatgtta tctaatgtat ttttaaataa ataatcatta ttatcaaaat aatgttaaaa
    14161 ataaaaaaga attgtccatc actgttattc aaatcatttt atcaaaacta catagtaaat
    14221 gttaaaaagc aacatgacat aatatataga aggctttaca gtatatatgg gagcttacta
    14281 aaaaagaatg gagtaagagt taacacaatg ggccattagc tgaagtttta acagaattag
    14341 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtatg agggttaccg
    14401 ttggtaact
//
LOCUS       DACCPY010000074        14038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCPY010000074 DACCPY010000000
VERSION     DACCPY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.076"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IVO69_002017"
     CDS             140..460
                     /locus_tag="IVO69_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400783.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IVO69_002018"
     CDS             912..1112
                     /locus_tag="IVO69_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7400784.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IVO69_002019"
     CDS             1259..4048
                     /locus_tag="IVO69_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7400785.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKMPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IVO69_002020"
     CDS             4095..4622
                     /locus_tag="IVO69_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7400786.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IVO69_002021"
     CDS             4643..5833
                     /locus_tag="IVO69_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7400787.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IVO69_002022"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IVO69_002022"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7400788.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IVO69_002023"
     CDS             8224..8898
                     /locus_tag="IVO69_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7400789.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IVO69_002024"
     CDS             8895..9236
                     /locus_tag="IVO69_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400790.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IVO69_002025"
     CDS             9266..9625
                     /locus_tag="IVO69_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7400791.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IVO69_002026"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IVO69_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IVO69_002027"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IVO69_002027"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7400792.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IVO69_002028"
     CDS             12541..14007
                     /locus_tag="IVO69_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400793.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4651 a         2430 c         3004 g         3953 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatgcca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgac
//
LOCUS       DACCPY010000075        14024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCPY010000075 DACCPY010000000
VERSION     DACCPY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.077"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(184..1128)
                     /locus_tag="IVO69_002029"
     CDS             complement(184..1128)
                     /locus_tag="IVO69_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7400794.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1262..2653)
                     /locus_tag="IVO69_002030"
     CDS             complement(1262..2653)
                     /locus_tag="IVO69_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP7400795.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2779..3189
                     /gene="tagD"
                     /locus_tag="IVO69_002031"
     CDS             2779..3189
                     /gene="tagD"
                     /locus_tag="IVO69_002031"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7400796.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3365..3778
                     /locus_tag="IVO69_002032"
     CDS             3365..3778
                     /locus_tag="IVO69_002032"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7400797.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3935..4192
                     /locus_tag="IVO69_002033"
     CDS             3935..4192
                     /locus_tag="IVO69_002033"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7400798.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4279..5517
                     /locus_tag="IVO69_002034"
     CDS             4279..5517
                     /locus_tag="IVO69_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7400799.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDEI
                     RAYTRESLKQYDLVEVADEE"
     gene            5507..6247
                     /locus_tag="IVO69_002035"
     CDS             5507..6247
                     /locus_tag="IVO69_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7400800.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6657..6821
                     /locus_tag="IVO69_002036"
                     /pseudo
     CDS             6657..6821
                     /locus_tag="IVO69_002036"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6888..8315)
                     /locus_tag="IVO69_002037"
     CDS             complement(6888..8315)
                     /locus_tag="IVO69_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7400801.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8312..9454)
                     /locus_tag="IVO69_002038"
     CDS             complement(8312..9454)
                     /locus_tag="IVO69_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP7400802.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9458..10453)
                     /locus_tag="IVO69_002039"
     CDS             complement(9458..10453)
                     /locus_tag="IVO69_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7400803.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            complement(10567..11556)
                     /locus_tag="IVO69_002040"
     CDS             complement(10567..11556)
                     /locus_tag="IVO69_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7400804.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            complement(11528..12358)
                     /locus_tag="IVO69_002041"
     CDS             complement(11528..12358)
                     /locus_tag="IVO69_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7400805.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            complement(12396..13787)
                     /locus_tag="IVO69_002042"
     CDS             complement(12396..13787)
                     /locus_tag="IVO69_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7400806.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
BASE COUNT         4746 a         2540 c         2078 g         4660 t
ORIGIN      
        1 tgatcattaa gtttggcttt tatgtcataa ttgaatttta taaagttctc tttttcatgt
       61 aaaatattta attatatatt tcctaaataa aaagtaaaac aagaattaaa tagttggtta
      121 tacaacttta attcttgttt tactttttag aattatttat ccaaagatgc aatcattttt
      181 tcatcaaatt tctgtcttta aataccgttc cattccgctc aatatatcaa atttaggctg
      241 atagcctaaa gaacgaattt ttgaaatatc cgctaaggaa tcacgaatat cccctgaacg
      301 ctccgcttca tatttaagcg ctagctctgt tcctaagatt tgatcaattg aatggatcaa
      361 ttcaagtagc gtagtgcttt ttcctgtacc gacattgaat tgctgtccta aagctttttc
      421 ttccttggca acaagtaaaa gtgcttgtat aacatcttct atataaacaa agtctctaga
      481 ctgactccca tcgccaaata aagtaaattc cgctttttct cctgccaatt gttttttgta
      541 acgatccact agaatcgaaa tgactccgct atatggagag tttggatttt gatttggacc
      601 ataaacgtta aaaaatcgga cagcactagt cggaacatca tataaatgac agtaatccaa
      661 aacatattgt tctgctgcaa atttatcgat agcatatggt gttaatggac ggatgactga
      721 ttcttctttt tttggcagtg ttggttcatc accataaact gcagctgatg aactaaatac
      781 taatcgtttt aaattagatt gatatttccg tgctaactca agcaataaaa gcgcactgtc
      841 aaaatttaca cgatgtgttt ctacaggccg ttctacagaa tctgccacac tagctacagc
      901 agctagatga aaaatatagg caaactgata ttctttcatg attctttcca tcaattgaga
      961 atctgataca tccccctcaa tgaaagtcac ttgtttatcc atatccaagt tttctttttt
     1021 tcccattgat aaatcgtcaa ctactacgat tttgttttct tttcccaaat aattcgctag
     1081 cgttgaaccg ataaaaccag ctccgccagt aatcaatata gtgtccatca aattcactcc
     1141 aattgcacat tttcgtatca aataatatta gtttaccatt tttatattgt aaaaactata
     1201 aaatttgttg aagttgggat aactagtttt cttttctact ttcatatacc taaaaatagt
     1261 ttcaattagt tactccttct tctttagata tagttttttg gatagaaccc agatatcccc
     1321 aaaaaataaa tgaacctatc gtactaacca aaatgagttc tgtgataaat aatgccgaaa
     1381 ctgctattgt aagaatcatt aaaatagcaa aaaagtgctt atcttttact agatttggtt
     1441 gtgtgaaaca ataacgcaag gctttaaagc attggataaa taagaaggtt aacaatgtca
     1501 atagacctaa tcctcctgta gcagccatca ctaagaaaat aaaattatga gcagttaaag
     1561 tatcttttcc cattaatgta tatggtagtt tatcatgtgc atattcaata tagtagcctg
     1621 gagaacctcc ggatatcggc ttactcttga atatttcaat tgcacttttc caaagttcaa
     1681 accttaaatt agaaacatcc ccgctatcag tgtcagggcg ttttaaatca actttattgc
     1741 tgctagttgt tcctgatgaa tcagatgtat ctttttttac ttctgtaata tgcggaacat
     1801 gtgcccatgc atgagcagtt atatctgtta cataatacat cgcaattata cttagcaatc
     1861 ctactactaa tgcagttagc catcttttta aagtattagt tttaagaaaa cgactactat
     1921 aacttgtaaa gaaactaaaa acaaaagttg ttattaataa acaaactaat gtggtgcgtg
     1981 aacccgataa agcaataaac gagaattgaa taaaaatatt aattacaaaa aatattttaa
     2041 atagaagatt taaatgcttg taagtgacca aataatatat agcaataatg atgacgatta
     2101 atgatataac cgcaccaaaa tttggatctg tatatacacc aaataaacgg ttgcctaacc
     2161 accctaaacg aactgggtaa tacttatatt ttattggaat ttttatgcca tattgaatga
     2221 caaaccagta taatgatact aaattagcta aaaaccatac caatgaaagt atccaggtaa
     2281 atactttgaa aaaaaagtta ctactatagt ctttgccaaa ctgataaatc ataaagaaaa
     2341 agattaattg ccacatgatt aacttaatat tttcgctata tccatatttt aaattcatta
     2401 aactgcttac tagcatcgcg ccaataaaag caattaatag ccaatcatat ttttttagat
     2461 ttttttctct aaatgcaata attgcatcta taatgatcaa gacaactccc gctattccag
     2521 caggccaaaa aagtaaagta tcatatctag taggtaatag taaatacggg cctattgctc
     2581 gtaacattaa aataaaacca aaataaataa aataccaacc tttcacatat tttaaaaatc
     2641 tttcattcaa catttttcct ccatccacct atttctcata aatatttctt atccttttta
     2701 ggataaactg gtcctttcaa ttctaacaaa aaaatgctaa actaacaatg tatattaatg
     2761 aatttcgaga ggtgaaagat gaaaagagta attacttacg gtacctttga tttgcttcat
     2821 tacgggcata tcaatttact gaaacgagca aaacaatacg gagactatct tattgttgcc
     2881 ctttctacag atgaattcaa ttggaatgcg aaacaaaaaa aatgttattt tagttatgaa
     2941 aaaagaaaac agttacttga agctattcgc tacgttgatt tagttattcc tgaagaaaat
     3001 tgggaacaaa aagtaaaaga tataaaagaa taccatgcag attatttcgt gatgggcgat
     3061 gattgggctg gacattttga tgaacttgaa caactaacag atgcacaagt gatttattta
     3121 ccaagaacac ctgagatctc tacaactcaa ataaagaacg aacttaattc tttaaatagt
     3181 gataaataat attttttaaa caataaaaga acaatatatc tggataagac gtaaaaaaat
     3241 ggctctataa tttctaggac tgatagaatt ttctgtcggt cctagatttt ttgtaaaaaa
     3301 aaacagacat aaaaaaattc cgatagaata gagtcgtcta aaacatcaat ctgaaaggaa
     3361 gtttttgata tccatttctc attctatcaa agagctcttc actattaaag ataaaaatat
     3421 tgaaattaat tcttttcaag aaacttttta taaggacaag aaagccatcg taatcgaagg
     3481 tccattagtt cgcactttta gacattgtct attttgtaaa gaatctgcaa agcagatcga
     3541 gaaaaacggc aagaagattt caatgattat cttaaatcgc agtgggaata aacaaaccta
     3601 tttgcgtctt agaaaacaac attaccactg ccaatcatgc gggaaatatt ttactgctca
     3661 tacgtatctt gtcgctcctt catgttttat ttcgaaacaa gttcattaca cgatattaga
     3721 agagttgacc gagcgtcaag cattaaagac aattgcgaaa cgttgtgatt ttcagtaacg
     3781 acagttcaac ggacattatc tgctctatcg gcaacgctgc gacagaagct agactattta
     3841 cctcattgtt tactattcga cgagtttcgc tctctttcaa ttttccatgg gaagttctcc
     3901 ttttcttgta tgaattgaga gacagggaag ctctttgaca ttttgtccag ccggagaaaa
     3961 aaaagatttg attgcttatt tcatgcgttt tacaagaaaa gctcatatgg gggttcgata
     4021 cattgtcaca gatatgaatg ccccttattt ttcattagtc aaagaaccct tcccaaatgc
     4081 acaaatcatc attgattgct ttcacattgg gcaacacttg aacaagtttt tcgatcgtgt
     4141 tcgaaaacgt gtgatgaagc agttgaatca aaaatgtcaa cggcaccttt aaaatgtagg
     4201 aaaacggcaa tttaaaattg ttggtttacc tacatttttg tatgctcttc ttatcccaaa
     4261 tgaaatggag gctcagggat gagaaaagac atacgcgaag gagtgatgat ctacgtgatc
     4321 aatgaaatca aacccaatta tgctgcacta gcaaaacaat acgactgcga ctaccgaaca
     4381 gttaagcatg cttacgaaga agcccaagtt aaagaatcaa aacctccaga acgtaagaag
     4441 cgtccaagta agttagatcc ctacagagaa atcatccaag acaaaatcaa agaccaatgc
     4501 agtgcttaca gcattttcaa gtttattgag cacaaaggat ttggaggaag ctacagttta
     4561 gtaaagacct attgtcgcca gattaagcag gcgaaagtac acaaggcaac cgtccgagtc
     4621 gaacatagcc ctgggctttc tgctcaggtt gactggaagg aagatatgcg actaatcagt
     4681 aatcagggcg aaatttttga gttcaatatc tttttgtacg tcttgccata ttccaagaaa
     4741 aaatatgtca cactaacttt cgaccgaaaa caagacacac tctttgagtg cttagatgat
     4801 gcgttttatc aaaccggtgg cgttcccaaa gaaatctggt tcgataatat gaagacagtc
     4861 gttgaccatt ctcgtagtca gttttccaaa ccgcatttca acaaacgttt ttatgctttc
     4921 agtaaggatg ccggcttcac accaattgcc tgtcggccat ttcgtccgca aacaaagggc
     4981 tgtgtcgaag ctttagccag aactgttgac cgcctacggg tctataatca tgaattttat
     5041 gatgccgttg agctgtgtca tttagtgaat aacctttgtg ttgagttaaa taacgaggtt
     5101 tctcaatcaa ctgatcaaac accagatgaa cgttggcaga cgaacgaaaa agagcacctg
     5161 catccatacc aaaaaaacct tttgaatccc tattttgaag actttattcg ccgtaaagtc
     5221 accaaggaag aaatggtcat tttccgtaaa tgcaggtact ctgtggcgcc tcgctatatc
     5281 ggtaaagaag tcgaaatcga actctccgat aaagaagacc aagtacaaat ctattataac
     5341 ggagaactga ttcggtcgca tccattaacg acaaaacagc tgaactacca cgaggaagac
     5401 tatttccaaa tcttgaaatc cgatgtcatg agtcacaaag aagacgatga aatcagagcg
     5461 tatactcgtg aaagtctcaa gcaatatgat ctggtggagg ttgccgatga agaatagcca
     5521 ttaccaagaa ctgatgacca acttagagac tcttggtttg aatcatatgc gggaatatgt
     5581 tcctaactac atcgaagtgg ccaatcgaga ggagctttct ttaacggaag ctcttctcga
     5641 gctgacacgc agagaagttg atcatcaaaa taagcaaaaa atgaatcgtg tggtggcacg
     5701 cgcccgcttt tccaagagaa ccagcctgga agagtttgat tttgccttcc agccaagtat
     5761 caataagcgc gaagtgctag acttaaggca catgggcttt gtggaaaaac atgagaactt
     5821 ggtttttatc ggcagtcctg gtgtaggcaa gacacatctg acgattgcat tgggaatcga
     5881 agcctgtcgt caaggctatc ggacactatt catcacctgt catgagctgc ttttgcgtct
     5941 gcgaacagcc tatgaaaaag gtaccctaga gcgtgttctc aaacgatacg ctcgctatga
     6001 cgtcttgatt attgatgaga ttggctatct tccgattcaa aaacaggaag ctgatttatt
     6061 ctttcagctt cttgccatgc gctatgaaca ccgctctacg atcattacga cgaacattgt
     6121 cttatctcgt tggggggagt tgttccaaag ttctgagatc gcagctgcca tccttgaccg
     6181 tttagtacat cacgtcaagg tgttcaagat cacaggcaag tcttatcgta tgagagacaa
     6241 gatctaaagt gtcgattgtc gacaattact attgccgaaa acctacattt ttatcctgcc
     6301 caaaacctac aattttaagc tgcccttgac atcaaaaaga cacttcacaa gctaagtgct
     6361 atcgacaatt gaaacctttg tataaattac ttctataatc agatgacgag ttggattata
     6421 caacattcaa gaaatggcag aattttcaat ggcgctattt aacagaaagt gaagtagttg
     6481 attgtctacc ctcaatctct tatgaactta aaatggctta gcagtattat caaaagctgc
     6541 ttaacaatta tcacaagaaa cagctagcta cttcgagaaa tgaccaaaga agttccctcg
     6601 aaacttcact atcttaaaaa gcctttttca tatctgaaaa aggcttttaa ctagcatttt
     6661 cgaaaaagta ttttaataca aaattagaga atcttcgcac acatatcaaa acattgaaac
     6721 gatttgctta tggattttgc agttttacca atatgcgctt gtggattttt ctaatcaacg
     6781 ggttaataag ctatgcatta aaagctagta aatcttacta actttgttga tcataaatga
     6841 ccttatcagc ccgatttgac aaagaactac ttttttacaa cattatttta ttttcgaata
     6901 atctttctta cttcatttcg tatatctgga aaaagaaaat aaacaaacgg aagataaata
     6961 atgaaaatca ttattgattt aattgccata tctaaaaatg aagaagagaa taattgtgga
     7021 agaaagtaat aggccgaagt agtcaaagta agattgacta taaaaaataa gacaacgttc
     7081 ttcccaaaga aataggatat ttttatttct tttcgtacaa aaaatactct aaagaaaaag
     7141 actaataatt ctactaccac gagtacgatt gtagccgcta cagcgccata agtaggcaca
     7201 aaagctacat ttccaataat acttacaatc gcaccaatga tcattggata agcatactcc
     7261 ttatcttttt ctatagcaat tgcaaattgg ttagcaaaaa ctccaccaat tggactaaca
     7321 actataatta gacaagaaat aagcatgttt attgtcatcg gtttgaaatt ctccccaaaa
     7381 aaccatggaa caaattcttt tgtattgata aacaccacac taaaaagaat taatgcaata
     7441 ataatcgtgt aatcaaaaga tttttttgtg taaactaaaa tattttctga ttttccctct
     7501 gcctgtgctt tagatatttt aggcaacata acaatagtaa ttgatgttaa gactgccaat
     7561 ataatacgag caattttttg agactgatca taataagata atgctgttgg atccacaaaa
     7621 tacccaacta tcgttttgtc taaagttgtg tacacttgca taaacaactg aggagtcaaa
     7681 ataataaaag ctggtttcca caactgttta aaaacattag agttaaagag tggtttacct
     7741 acttttccgt ggatactcaa ccagaaaact aaacttccta aaaagttagt aattgaagta
     7801 atgaacacat aaatccacaa atcatcttta gttgaaacaa aaaggattat taacaatacc
     7861 gatgataatt tgataactgt atttctaata accactttcg aaaattcttc aattccttga
     7921 aaaaaccaag aaatatctaa tgttagccct gcaataaata tcaattgaat taagtataaa
     7981 tttttatact ttacatcaaa aaaataaata taggctatat agagaaagga aacactcaag
     8041 cctattaaaa attgaagaaa gtaaatattc cagaattgta aagatagtgc ttttttatct
     8101 ttccttgatt tagcgatatt tcgaatacga aaaggattca ttcccacagc aatgacagtc
     8161 attaaaaatt gtgatattga gaaaacatac ccataaatcc ctaaaccagt tgaacctatt
     8221 ctacgactaa gtataggtat agtaagtaag ggcaaaacaa tcaaaaagac ttgatatatc
     8281 gcattataaa atatatcttt tactgttttt ttcactttat atccctctct aacaactcaa
     8341 gaaccttttc tgtacttgcc cctgtttcat aatttccaat ataatcaagt aactcttgag
     8401 aacttttcaa aagcttttct tgttggtttt taaaaacatc aacaaggcca taactatctt
     8461 gtacgcttat aattggtaac tcacttaact gtatataaaa acctctttct aagctatatt
     8521 ctttttcatc atagttaaaa agaataattg gtttttttgt tagcgcaaaa tcgaaaaaaa
     8581 tagatgaata atctgtgatt aaaacatcta tctcttcaaa aagcatttca agaggaatcc
     8641 catcattaat taaagtaatt tgatgttttt tcatatattc tatactttta gaatcagttg
     8701 ctaatttact atgaacatta ggatgaaatt tagtcattaa atttaattct tgttgttgct
     8761 ttttaaaaaa ttcacgtaag ttatccacac ctaaccattc tagttgcgta attccgttgt
     8821 cctcaatatt atttcgaaaa gttggagcaa ataagacatt gatttgtttc tttttcaagt
     8881 caaacacttg ctctttacta gaggctaatt catcgtttct aggagttcct acttctaata
     8941 ttttagaagg aacgatattg aatgctgaaa catacttctc agaactatag cgattaccag
     9001 ataataaata tgtaattgac tcactatctt ttttcgcata gcttaggtaa cgtttaggta
     9061 acttatcttt tgcatcaaat tcaatttttt ttaaaggcgt accgtgccat gtttgaaaat
     9121 aaaattgttt tttccttttt gcatacttaa tcgttttccg actatcgtct atccaatact
     9181 tagctgtata caaataaaag gcagctaaaa aagaatcgga atcaattctt ttaatataat
     9241 ctggtaaatc agttataatc gcttcttttt tgattaacca aataattctg taattcaatt
     9301 ttttttcatg aattttctct gaaataactc ttggatttcc agaataatta cgcccgccaa
     9361 aacttgaaaa tacaattaaa tttttttgaa taggtaatat tttaataaaa accgggaaaa
     9421 tattccttga aaaaaatttt tttatattct tcatctctta cctcacagat tctatcttaa
     9481 tcaaaaattt acttgctatc ttactacttg acatatagta aggcaagttt ttatttatta
     9541 ataaaaactt tactaatttt tttaattttg gtgaaaagtc gggatcaatt ttcacttctt
     9601 tttcaaagcg ttgtaaaata tttatagaac gtaaataatc ttctttattg gtatagttat
     9661 ttttaatcat tgtcatcatc atccttattg cttcatagac atagcgagtt ctaacattaa
     9721 ttgataattc cggataaaac ttatctatga atactagtat atcttcatgg acttcaaaag
     9781 aatcaaaaat tctaggattg aagcttcctc tcagtatact tttagggctt tggtaatagt
     9841 gatatttagc agatgaattg acagcaactt tttcggaatg atcgatcaac aaataagtag
     9901 tagccttatc ttccattaat ttacccagtg ggaatcttat attatcaaat aaatttctat
     9961 tgaataattt attatttgca gaatagccaa ttttattatt tataaataag tttttcattg
    10021 cttgttctct atcaaatatt tcccattttc tctcagcaat tgtatgttct tttagttgtt
    10081 ctaaacttga aatctttttt atttttagca ccgagatatc tgctccataa gtttgcgtac
    10141 caaaatatag taactcaaga tagtctggtt ctatccaatc atcgctgtca ataaagcaca
    10201 caaaatctcc tgttgcagaa tcaagaccag aattcctagc cgcacttaaa ccaccattca
    10261 cttgatgtat cacttttaat cgcatatctt tctttttata ttcttctgca atttccccac
    10321 ttcgatccgg ggaaccatca tcgactacta ttatttcaat atttgaatgt gtctgattgg
    10381 aaatagattc taagcagttt tctaaatagt tttctacgtt atatgcgggt actataacac
    10441 taattttatg cactctcttc acttcctaaa aatcatcttt tttattttta caaatgtatc
    10501 attaccagta acaattttag tcttccgaag ttaataaaca taaaaaataa catatcgaaa
    10561 aaaactttat ttttttagaa cttttttata taaaataaaa taagttttag gattaattaa
    10621 gtataaaaat aaagctaatt ttttagcaat tgatatctca ttgttacttc ttatttttga
    10681 atatatactt tttatttctc ttttagactg attataatat ttttgttttt ctttctgatt
    10741 ttgatttttg ccagtaattt taaaccctgt tgaaagatat ccgtctacta accgagcatt
    10801 ggccaaatta ataagtcgag gataatcttt ctttacttca tcaactaata tttttctctg
    10861 caaaatatta tcatactgtt ttgaattaaa agactgataa acgatgctat cgttattttg
    10921 ataataaaaa atacctgaaa atggcaaaat atcaatttta ctagccgccc taaaagcttc
    10981 atacatagga accatatcct catataattt attttctgga aaaactatat gattgaataa
    11041 ttccctttta aaaaatttat tccatacgta aaagtccact gccttacttc taaacatttg
    11101 ctcaattgct tcttcacttg aacaaccaat cttcttttcg ggaacaaata aatttgcatt
    11161 ttctatattt tttcctttac ttaatccaaa agcaatgata tcggggtgat tcctatggta
    11221 gtctttcaaa acattattaa aacaatcagg taaaaaataa tcgtcactat ctaaaaatcc
    11281 taccaaattt ccagttgctt cttttatccc atagttccta gcaactgatg gaccagcgtt
    11341 ttccaagtga taacatgcta ctttactctc tagattagca atttcgtcac caatgtcact
    11401 actaccatct gtagatccat catcaattaa caagatttca atattgggat aaccttgcgt
    11461 taagacgctg tctacagctc ttcttaaagt attctttgca ttgtaaaaag gtatgattat
    11521 agaaagttta gtttgttttt ctaagttttg attcatatac ttttccccta attttattcg
    11581 gtaataaact aattacaaga tgtccgcaaa taactatcag aaaatctttt agtgatccta
    11641 gaccatcttt ccatacgcga tatcggcctt taattcctgg taaaagatat ttacttcctc
    11701 cacgtctagc atacatgtca gcacctgctc tagcatgtac tagaatctct tggatattat
    11761 atcctttata tcccgccttc aatagtctta cccacaaata ataatcttca aatccaatta
    11821 atgggcgata gtttcccact tctaaaatag catttttatc aaacatgact gtcatatgat
    11881 taaaagggtt tcttcttttc gaaaacacac ggatttcatc attatactca ggtacaatcc
    11941 ttcttccgat aacattgcta agtgtgccag taaattcttc aatattactc ccaacaatcg
    12001 ctatttctgg atattcttgg aagatctcta attgtttttc caatctgtgt ggttccatga
    12061 tgtcatctgt atccattctg gccactaagc gatttttagt atgttctaat ccagttgcta
    12121 aagcaatccc caatccttga tttttttcta attttacaac tgtaaacaag tgaggaactt
    12181 tttgctggaa ataagtgaca actgcctgaa gcttctcatt tatagggcca tcataaacca
    12241 atacgatttc tgccggcatc accgtttgct caaagatact agctaatgct tcctttaaat
    12301 attccggctt ttctttatca tacaaagaaa gcagtatact tatattttta ttttccaatg
    12361 ttctctccta cttctttgct caaaattttt ccaatctaac gatacgtttt ttcttttgga
    12421 aatgcacttt ctgctaatga actgatattt tcatcttcat ctagtccttg tgaggacatc
    12481 ttatcaaata agattttgat tgtttgaagt aaaatcttca aatctaatag taaggaatac
    12541 tttttgatat acaacaaatc aaaacgtagt ttactgttat aatctgatac atattttccg
    12601 tatacttgtg catatccggt aattcccgca cgtacattgt gtcttaagta ataataagga
    12661 ttttcttcat tgaactgatc aacgaaaaat ggtctttctg gccttggacc gacaatcgac
    12721 atatcgcctt tcaatacatt aaagatttga ggtaattcgt caaaacgtac agcacggata
    12781 aatttaccta ctggtgtcac acgtgcatcg ttacttttag ccaagactgg tcctgatttt
    12841 gcttcagctg tcgcactcat ggaacggaat ttatagattg aaaattcctt ttgatttttc
    12901 gtaatacgta cttgtttata gatgattggt ccaggtgaag ttgtttttac caaaatcgct
    12961 gtgattagca taaaaggtga ggcgaggatc agtaaaataa gcgaaacgat gatatcaaag
    13021 agacgcttga atagttcgta ttctggttgg attctgaacc cagaagcttc aatgatgctt
    13081 tcatcttcaa aattcataat attcggattt atcattgtca gattctcaaa agtcgtattt
    13141 aaaaataatt tcttctcttt tttagtcact aattgataaa tgttgatttt ttcatcttct
    13201 gggattttag aagccaaata gacgatatcc atatcatcga tcaatttttt tgtattttcg
    13261 taataattat cgattgccac tgcttcaacc ttatgtttat tgtttttctt agattgaaaa
    13321 ttttggatag ctggtgcgac atctttctcc aaacccacga taaggacttt cttatcacga
    13381 ctgactctta agtacagttt gtaaacgact atccggtaaa cactaagtaa tatcgtacct
    13441 aggataaacg agatcaaaat gacgccacgc ggaaatgcaa accatcggcc agcaaaagta
    13501 actaccatga tccccaatgc tattagtact tgtccaataa cagtaatgaa gacaatatca
    13561 ctgatcattc ggttatagaa tacataagct cctaacaaaa tattcaacgc aataaagatg
    13621 atggaaataa aaattgctga atgctgaaag gtttctaagt ttctcactgg tacttctccc
    13681 tggaatttca gcaaaaaact aagatagatc gatacattta atacaagtac atccataaga
    13741 ataatgaata ttctgcgtgc cgcattccac tccccatttt tattcatata tacactccta
    13801 accataaaat aatgcctcta tcatcttacc atagcaaagt ttaaaaacct agtgaataac
    13861 caaattgtaa aggaaattaa aagaaacggg gaaaagcgtt tattgtctgt ttatatgtgt
    13921 atttctgcat aacagaaagt tgtctcagaa gtcccatcac ttcctttatg ctgaactagt
    13981 attctcctgc taattcctta ttcacaagtt acaatattca tata
//
LOCUS       DACCPY010000076        14008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCPY010000076 DACCPY010000000
VERSION     DACCPY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.078"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(140..796)
                     /locus_tag="IVO69_002043"
     CDS             complement(140..796)
                     /locus_tag="IVO69_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7400807.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            989..1267
                     /locus_tag="IVO69_002044"
     CDS             989..1267
                     /locus_tag="IVO69_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400808.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1286..2494
                     /locus_tag="IVO69_002045"
     CDS             1286..2494
                     /locus_tag="IVO69_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7400809.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2642..3007)
                     /locus_tag="IVO69_002046"
     CDS             complement(2642..3007)
                     /locus_tag="IVO69_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7400810.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3154..3927)
                     /locus_tag="IVO69_002047"
     CDS             complement(3154..3927)
                     /locus_tag="IVO69_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7400811.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4101..4961)
                     /locus_tag="IVO69_002048"
     CDS             complement(4101..4961)
                     /locus_tag="IVO69_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7400812.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4961..6379)
                     /locus_tag="IVO69_002049"
     CDS             complement(4961..6379)
                     /locus_tag="IVO69_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400813.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6436..6885)
                     /locus_tag="IVO69_002050"
     CDS             complement(6436..6885)
                     /locus_tag="IVO69_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7400814.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7330..7593)
                     /locus_tag="IVO69_002051"
     CDS             complement(7330..7593)
                     /locus_tag="IVO69_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7400815.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7692..8987)
                     /locus_tag="IVO69_002052"
     CDS             complement(7692..8987)
                     /locus_tag="IVO69_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7400816.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8984..10255)
                     /locus_tag="IVO69_002053"
     CDS             complement(8984..10255)
                     /locus_tag="IVO69_002053"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7400817.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10420..11289)
                     /locus_tag="IVO69_002054"
     CDS             complement(10420..11289)
                     /locus_tag="IVO69_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7400818.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11581..13191)
                     /locus_tag="IVO69_002055"
     CDS             complement(11581..13191)
                     /locus_tag="IVO69_002055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7400819.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3987 a         2953 c         2390 g         4678 t
ORIGIN      
        1 tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
       61 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaacgctgat
      121 ttcacaacct ctttgttcat caaaagataa tattgtcttc taacaagtcc atattcaaaa
      181 taacgatatg atggtcttta acggtgataa taccatcttt tttcagttgt tttaaaattc
      241 tgtttacact gctagcagtt gagatgccac aaaatttggc gatttcttca ttcgtgatcg
      301 aaaaatcgat cagcatccct tcttctgtcc gcacaccgaa taaatcatat aattcgtaaa
      361 gttgcgtaca aacagctccc agctttccgt ttaatagcat ttgctgcatt tttttcatcg
      421 aataaagcag acgatagcgg taatactctt ttacatagtg ttgtagcttt gggtcttggt
      481 tgatattttg ccaaaagcgc acacgattga ttcgataaaa ctctgcatat tctgattcaa
      541 ctcgaatatt atacggcgaa tcgatgaatt gtgaatattc gtcttttaaa agagagacga
      601 tttcaagatc tttgatatag cgaaggttaa attctctccc atcatttgaa ataacacttg
      661 cttttaccgt tccctctttc aagatatacg tatgggaatc ttccatccct tcgaatgtca
      721 aatatttatg atatcctttg cgtataatcg gaaaatcatt tttatccaaa tattccttta
      781 agactctact atccaatgat gctcccccat ccaaactttc atctaaaaag atcatacagt
      841 actttaaaac tttttaaagt ccaaaggtat tttactaatg atattaacat ttgttaagta
      901 cctttttcca tcacttatgc gatttttttc ctttttttta ttcgttatac tgcagaagta
      961 aaatttttta agaatagagg gaaaaaacat gaattactgg atgaacacaa tcattaatcg
     1021 attagaaaca gcatatcaaa caagatttga tatgaaagct tctttagtct ttttaaatga
     1081 tgcgtatcaa aactcgatcg aattgatcaa agcagtggat gagaatccaa caaacgaatg
     1141 cgaagaattt ttaaatctgt ttatgtctac aagagattta tttattcgcc aacttgttga
     1201 tcgctatcca tccaattatc acgatgtaga agttcaaata caaaaattaa aagcatattc
     1261 agcttaaggg gaatgtataa catacatgga aaagaaatta tttaataaat attttattgg
     1321 aatcacattg atcaatttta ttgtctacct gatttattat ctgttgatgg tcatcatggc
     1381 agtcattgcg caagacacct tacacgccag cttgagccaa gcaggttttg cttctgggat
     1441 ctatatcatc ggaacattgc tggcacgctt attcatgggg aaacagttag aactatttgg
     1501 tcggaagttt accttaagag gcggcgcgat cttctatctc ctttcaacat tagcatatct
     1561 ttatacgcca actatcgggg cattgtattt agttcgattc ttgaatggat tcggctacgg
     1621 tacagtttct acagctacaa atgctatcgt tactgcatat atccctgcat ctaaaaaagg
     1681 ggaaggcatc aattattatg gattaagtac tagtttagct gccgctgttg ggccattttt
     1741 aggaatgatt ttgctgaata tgactgattt tcgtttcatt atctggtttt caattgtact
     1801 ggtcttcttt gttacgattg cctgccttgt ctttcctgta aaaaatattc cattatctga
     1861 agaacaaaaa atatctcttc gctcatggaa tatcaatagt tttattgaaa agaaagcact
     1921 tttcattaca ggaattgcct ttttgatggg tctttcttat tcaagtgttt tatcattttt
     1981 atcttcttat acaaaagtaa tccacttagt tgctgctggg tcatttttct tcgtcgtata
     2041 tgctttggtc atcacattta cacgtccatt gactggaagg attttcgatg tcaaaggaga
     2101 acaatacgtg atgtatccta gctatttatt cttaacagca ggattatttt tactgagtgt
     2161 aacgacgaat agtgtgacat tgcttatctc tggagcatta gtcggcctag gttatggtac
     2221 gtttatgtca aatggacagg ctgtttgttt aaaaatcgtc cgcgaacatc gaatcagtat
     2281 tgctttatct acctacttta ttggtttaga cctaggctta ggcgttggtc cttatttgat
     2341 ggggatgcta aaatctctta cttcatttag aggattgtat gtgattgccg gagtcattcc
     2401 attgatatgt acagcactct atcttgttaa tggccgaaaa catagtcatg aagcagaggg
     2461 cgaattgatt gaatcattga aaaatgaaat ttgatcttga tgaatagata aaaaaaaccg
     2521 ttcagatctg aaaataatct gagcggtttt ttgagtaaaa atagtttatg attattctta
     2581 attaaacatc tatttatttt taaagctttc atttatcgat aagaatagtt taactgttga
     2641 tttattccat tttccgttca tctggtacga tgccttgact tagcaaccac ttgtggaaga
     2701 accattctgc cacaccgatc aatagagcag gaatcaatac tttcatccaa atatcgactg
     2761 tatcattcat aaaatataga tagagccaca agataactgc agctgaacca gcatctgcga
     2821 tcgtcgctgc gatgttccct actttacgta agatcaacag atcccctagc acatagatca
     2881 tgactacaat aattacccca acaatcgttg aatccataaa gctaacaccg tataaacctg
     2941 tcagcacgac ccacagaatc gcccagatca tgattgcctt cactaccaac gccttcacat
     3001 gtttcatttt ctgcactcct ttctcctgga taaaaaagta agattatcta acaagataac
     3061 cttttcatta ttataccatc ggctgagaga agcgagcaaa gataaggtcc tatctaaaaa
     3121 tgaggaagcc acttagaaca ttcacgtaat aatttatccg tacagacatt cgatatggtg
     3181 ttcctttact ttcgcatact gttcttttgt cagttcttta cttgtcacca gataatcgat
     3241 ttcatgaaaa gcaatggctt gatgcatgaa tttatgtgca attttttgct gtgtaactaa
     3301 aacggcaagc cggtctgatt gttttctaaa agcttttttg accattgcct ctccttcatg
     3361 agtttctgtt actccttggc tgaaatctag cccgcgacaa ctcattatcg ctaaatccgc
     3421 ataatatgac aatgcatcgt tataggcttt tgaaccgttg attgttgctc gtttcggaac
     3481 aattgcccct cccaatacag atacttttgc cgttgtatat tcactcaaca tagcagctgt
     3541 gacgatgcca ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc tgactaagtg
     3601 taaagaggtt gtactggaat ccaagaataa gaccatatca tctcgaatca aagaagaagc
     3661 tgtatctgca atcttacgtt tttctgtttc ttcttctaat ttggtgatct tcatcaggta
     3721 aggcacgtca atttttcgat tgtcaataag catggcgcca ccgcgaactc tacggatcaa
     3781 tccacttttt tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat aatttactaa
     3841 ctcgatcaaa tcggtgactc ttgcaaattg gttgtttttt agataattca caatctcctg
     3901 ctgtctattc tccacttcat tcaacataaa atcaccccat ttactttata tagtaacagg
     3961 aaaaaagagg ttgtgacaga agtgattggc ttcgagaaat aagaagaaat ttttcgaaaa
     4021 aactacttct gaacatcgtg tgtggttaag agggtatgac aagactattg tcataccctc
     4081 ttgaccacac atttttattt ttagtataaa ctagccttgc ctactgtgtt caaaatttcc
     4141 attttatgtt tgaccacttc ttgtaccttt ttattagctg ctggataaat tgcattcggc
     4201 tcgtacatcg atggattttc actcaatatt tctctcaaag cattgaaaaa gacgctttta
     4261 agatctgagc tcaaattcac tttggcaact ccgtatttta ctgcttcgct gacttctgcg
     4321 tccggattac ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg gttcaattct
     4381 ttcagtaagt cgatatttaa ttttggagtt ctgtttttcg gatacaagcc atgcgctgtt
     4441 ccaattgcta cagcaagtgt atcgattcct gtttctttca caaaaatctt cgcttgatca
     4501 ggatctgtat aaatgattgt ttcagcacct ccttcagcag aaccattatt cccaatcgtt
     4561 ccaagctcag cctccactga gacatttacc gcatgagcta acgcaaccac ttttttcgta
     4621 atctgtacat tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga agaatagccg
     4681 tttcgaatcg cccgtaagac atcttctatc gtaccgccat gatccaagtg gataacgatc
     4741 ggcagtttac ttgtataagc ataggctctt actgccgcaa caaactcatc acttaaatag
     4801 gcgatttcat caggatgaat ttctaagatt actggtgctt ctaatctctc tgcttcttcg
     4861 atgattgctt tcatcatatc atagctgcaa atattgaagg ctggtactgc aaattgattc
     4921 tctttagcca ctttcaataa ctctttcatt gtatataaca ttacgcttcc acctcatttg
     4981 ttagattttc atttttcgtc cacttgtctt gttttttctc tttcactgtt tttttcaata
     5041 gtccaagcat aaaggctgta gtgactgtac ctgatgcaat tgccaagaca tacataggta
     5101 aattgttcat aacatttgga attagcatca cccaaatccc tccatgaggt gccattgaag
     5161 taactcctac tgccatggaa atccctgcag cgactgctga acctgtcatc aaactaggta
     5221 tcacgcggat cgggtcagat accgcaaacg gtatagcacc ttctgtaata tacgaagcgc
     5281 ctaaaatcca gcatgattga cctgaagtcc gttcttcttt ggtaaattta tgtttgaata
     5341 ataaagtggc taaagcaata cctaggggag gtgtcattcc tgcagccata caagcagcaa
     5401 tcggtgcatt tactccagct gacatcaaac cgatcgagaa agtcgaagtc gatttattga
     5461 tcggcccacc catatcagct gccatcatga tcccgataag taatccgaac aatagcccgg
     5521 atgtatttcc caagctattc agccaatttg tcaaagcatc caacacccat gtgatcggtt
     5581 gttcaatcac aaataccatc aaaagcccaa taattaagat cgataaaaag gggacgacaa
     5641 tcagctgata aatagcagaa aattcttttt ttacgctaat tttatttgcc agaaaatcac
     5701 agatataccc tgctagcacg ccaccaatca ttccgccaag aaaacctgaa ccacctgtag
     5761 aggccagtaa tccggcaaca attcctgggg caaaacctgc ttttccagca atcgatgtag
     5821 caatccccgc gcctaacgca ggaaccatca tggcaaacgc tgtatctcct ccgatccttg
     5881 aaaatgcagc tgctaacaca ttatagtctg ctgagttggg atcagccgct gtaatcccaa
     5941 aggcaaaact cagtgcgatg atgattccgc ctgcgatcac aaatggaagc atgtaattca
     6001 caccattcat gaaatgatta tagattgtcg gctttttctc atttgcagct ggttcttttt
     6061 cattagacac aacgaatacg ccaatatctc tcaatgcttc gtctattact acttgcggtt
     6121 ctttgattgc tcggcttgta gagacacgct ttactttttt cccggcaaat cggtccaatt
     6181 cgatttcttt atctactgca agaatgattg catctgcctc tttgatttct tgttcagata
     6241 actgattttc tgtttttaca ccttgtgttt cgaattttgc ttgatggttt agatttcttg
     6301 ctgttacttc taatttttct gccgacatat aagtatgagc aattccagtc gggcaagcag
     6361 ttacaccaac gattttcatg attattttcc tcctttttct tcactatcga tactcggaca
     6421 aagactctac tattttcatt cttgactaat ctgtctgatg atatctgctg gcgtagtggc
     6481 ttcttttatt ccctttctaa aatctgcatt catcaatcgt ctggagagtg tgctgagaat
     6541 ctctagatgc atatctgctg atttttcagg aactacgatg gcaaaaatat attcaattgg
     6601 catttcatct atcccgctat agtccgttaa tttggctgta tgaaaaaaac acagtgctgg
     6661 ttttgtcaca tatttgcttt ttgcatgggg aatcccaaat ccatcttcta ttcctgtgga
     6721 gatctcctcc tctcgtttga atagtgcctt aacgactttt tgaacatttc ttaccttatt
     6781 tgtttttttc aacacatccc ctattcgctc aaataatgat ccttttgaat ctatcgatgg
     6841 atcgaaaaca atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa acctctcccc
     6901 cacaaaaaat gttaacgttt tcttggaact gaggtcagta tagcatcgct agctgtaccg
     6961 ttcaatatta tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata agtcattaaa
     7021 atgaacgatc tagctcacaa acaatcgcaa gtctataaca aaacaaaaag ccttccttga
     7081 aaggaaagct tagcttctaa ttttaggtaa atggtttttt acttcgaaat aacccctctt
     7141 catttcgtta atataaggtg tttttatcaa gtggataatt aaaactatat actagttttt
     7201 cattgttttg tgcattatga tcctacgact ttttcagcat caaacgaagt tttaaattgg
     7261 caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt tgagttatca
     7321 gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt tgaaacggtc
     7381 cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat gtgagtcaga
     7441 agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa ctgtttcttg
     7501 tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca tgaatactac
     7561 tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt gccctgctcc
     7621 atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac atgtttcagt
     7681 caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa ggagacatct
     7741 tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg gatcagttgt
     7801 tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat ttcttctaat
     7861 tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat atcgatggct
     7921 gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt tccagttcct
     7981 ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa agcggtcgct
     8041 aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa tttcttaggt
     8101 ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg tggaacgact
     8161 aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac atctcgttta
     8221 tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt atggttttgc
     8281 ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc tagatccgtt
     8341 acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg atagttttcg
     8401 gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg cggtgcgacg
     8461 atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg ggtagatagt
     8521 ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt taaggctgga
     8581 aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc cacgtgcatc
     8641 aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac tttgtcccca
     8701 tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc tgcacttggt
     8761 ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc caatattcct
     8821 tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag agataattcc
     8881 tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact ataaacttct
     8941 tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca gctcccactt
     9001 cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata tgatcataac
     9061 cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat agtccagcaa
     9121 tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg tgaagtttat
     9181 ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc attcgtgcta
     9241 gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct gaagtgcctg
     9301 tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat cctggataag
     9361 gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg atttcatctt
     9421 cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt tccaaatgtt
     9481 caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat gctagatagg
     9541 ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat gtttcgactc
     9601 cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata ttattcagta
     9661 aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact gtctgtcctt
     9721 ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc acgtccatga
     9781 aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca ccatgttcat
     9841 ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc ggtctagggc
     9901 ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca agcagtgttc
     9961 ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc ggaatcgata
    10021 cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt gcgcccatga
    10081 tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct tccaagatca
    10141 ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt tttgcaccgc
    10201 tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt ttcatctgtt
    10261 tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat agataaaaaa
    10321 aagaaacgag catggtctgc cgttttaagt aaaaaagaat ggagagcgac ttcaaaagtc
    10381 gttctccatt cctatatatc gtatctgtta tgtggatgct tacgctttgt tagctgaacc
    10441 gaaccagtcg atatgagatt tagcatcttc aacgattgct gctttacctg gagctaataa
    10501 tttacgaggg tcgaatcctt taccttcttt atctttacca gcttcgatat atttacgagt
    10561 agctgcagca aatgataatt ggaattcagt gttaacattt acttttgaga tacccattga
    10621 gatagctttt tcgatttgat cttgaggaat acctgatccg ccatgtaata ctaaaggcat
    10681 gtctggacct acagcatcag caattgcttg taagtggtca aaagctaatc ctttccagtt
    10741 ttctggatat tgaccatgga tgttaccgat accgcaagct aagtagtcga tacctgtagc
    10801 aaccatttgt ttgcattctt caatatcagc taattcgccg ttaccgatga ttccgtcttc
    10861 ttctccgccg attgaaccaa cttcacattc aactgagata ccttttgcat gtgctttttc
    10921 aacaacgtct ttagctaatt tcaagttttc ttcaaatggt agatgtgaac catcgaacat
    10981 tactgaagta tagcctaatt cgatacattc caaagcagct tcgtaatcac catggtctaa
    11041 gtggatagca actggaacag tgatgttcat tgcttcaact aaatcagtga ttaaatcttt
    11101 acagattttg taaccaccca tgtatttagc tgcacccatt gatgtttgga taagtactgg
    11161 tgcttgtttt gcttcagccg cttcaaggat tgcttgagtc cattccaagt tgtttgtgtt
    11221 gaatccgccg acagcgtaac cgcctttacg agcagctttt aaaaattcag ctcctgatac
    11281 tactggcata ataaaattcc tcctaagttt tgtaaaacta aatttgttaa gacaattctt
    11341 aaccaacgac cctattttac caaacatttc ggtagttttc tactaaaatc aatgaaaatg
    11401 aaaattttca agaagttttt tgcaaaaaat aaaatatcca atagcgcttt aatcgaaaaa
    11461 agtcttttgc tttctatctt ttttcctaca aataataaca aatcagaaaa agagaaaagt
    11521 gacaagaaaa ttgcttatcc ccttctcttg ctacttttct ctttctatca agttcaaaaa
    11581 ttattttgtt tcgtgatctg ctaatgatgc tccgacaaat gctttgatta atttttgcgg
    11641 gcgatttgga cgagaaatca attctgggtg gaattggcat cccacaaaga attttttctc
    11701 tggcagctct acgatttcta ctaaacggtt atctggtgag acacctgaaa agactagccc
    11761 ttgttcttca aacaattgac gatatttgtt attgaactca tagcggtgac ggtgacgttc
    11821 ttgcacaaca tcggctccat catatgcttc agccgtcttc gttccttttt tgattttaca
    11881 tgggtataat ccaaggcgta aagttccacc caaattctcg atgttttcct gatcagccat
    11941 taaatcaata atattgtttg ctgtatctgg attcgtttca gctgatgacg catcttctaa
    12001 tccaacgacg ttacgtgcaa attcgacaca agccatctgc ataccaagac aaatacctaa
    12061 aaacggaacg tcattttcgc gagcataacg aatcgcttcg attttccctt ctactccgcg
    12121 atcaccaaat ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt cttctacatt
    12181 ttctctcgtt aattcttgtg ctttgatcca atcgatctcg atgtctgaat tataggcgaa
    12241 accagaatgt ttcaatgctt caactactga gatatatgca tctggtaact cgacatattt
    12301 accgacaagc gcaatccgta cttttttctt caagttcaag acacgttctt ctaattcttt
    12361 ccattctgtc atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa caatttgatc
    12421 cattccttgt gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat ctggcgactc
    12481 gataactgct tctggttcga catcacaaaa ttgtgccaat ttgtttttca cgctttgaga
    12541 aacaggttgc tctgtacgta ccaccaaaat attcggttgg atacctaaac cacgtagctc
    12601 ttttacactg tgctgcgttg gtttagtttt catttctcca gcagctttca agtaaggaat
    12661 caatgttgta tggatataca taacattatc tgtaccgaca tctgctttca tttgacgaag
    12721 tgcttcaagg aatggcaaag attctatatc ccctactgtt ccccctactt ctgtaatgat
    12781 cacatctgaa tcagtcattt tagctgctcg catgattttt tctttgatct catttgtaat
    12841 atgaggaata acttgaacag ttgctcccaa gtattcgccc ttacgttctt tacgcaaaac
    12901 ttcagaatag atttttcctg tcgtcacgtt ggagtatttg ttcaaattga tgtcaataaa
    12961 acgttcgtag tggcccagat ccaaatctgt ttcagcacca tcatctgtta cgaatacttc
    13021 tccgtgctga tatgggctca tcgtccccgg atccacgttg atgtatggat caaatttttg
    13081 gatagttact tttaagccgc ggtttttcaa taagcgtcct aaagaagctg caacaatccc
    13141 ttttccgatt gatgaaacga caccgccggt cacaaaaata tattttgtca taagttcaaa
    13201 aactcctttt taatttatgt gcaggaaaag tctcagacaa taaacaaagc tccctattct
    13261 tcaataagaa tagggagccg aaatttctgt aaacttctga ccctctaaaa gggtgcccaa
    13321 ggtgtataat acaagctgac taagggaaag tcaagtactc aaatgaatac tatccgctta
    13381 caattttttc tttttcgata tcctttgaga aattatttct taagtccttt atgagtgcaa
    13441 aaaaaattta acccacataa taagcttgat ataaacatag ctaacgttaa acctaagcaa
    13501 atcagaaaat cgttactgaa aaattttaag tgaaataata gataaaaaaa taaaacaaac
    13561 attatgatag ataatatccc acctaacaaa gaactacttt ttcctacttg cagcaacagt
    13621 ttatatagct tatcgtagaa acaatctttt tctttcaccc ttatttacct cctttacgta
    13681 aaaaacgtag tccacagttc tcggattttt ggcatatctt ttctattaaa taatatcaaa
    13741 aacaaaacaa ttccactaat tttttcaaag atgacctttt tttcaacaaa agtgccttgt
    13801 agggattcat tgaaggtaac gaatcacata agtaaaaaat aaggagatgc gacaaaagtc
    13861 gtgcctcctt atttctaaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    13921 atatccatca aacatgagaa gctgtttttg aaattatttc ttgtgattcg gagttaaatg
    13981 gtaggctcac aacctctgga taaacggt
//
LOCUS       DACCPY010000077        13807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCPY010000077 DACCPY010000000
VERSION     DACCPY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.079"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO69_002056"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO69_002056"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7400820.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVO69_002057"
     CDS             complement(2260..2511)
                     /locus_tag="IVO69_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7400821.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVO69_002058"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVO69_002058"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7400822.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IVO69_002059"
     CDS             3461..3679
                     /locus_tag="IVO69_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400823.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IVO69_002060"
     CDS             complement(3968..4480)
                     /locus_tag="IVO69_002060"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7400824.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO69_002061"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO69_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7400825.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IVO69_002062"
     CDS             complement(6933..7367)
                     /locus_tag="IVO69_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7400826.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IVO69_002063"
     CDS             complement(7377..8165)
                     /locus_tag="IVO69_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7400827.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IVO69_002064"
     CDS             complement(8162..9103)
                     /locus_tag="IVO69_002064"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7400828.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IVO69_002065"
     CDS             complement(9280..9867)
                     /locus_tag="IVO69_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7400829.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO69_002066"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO69_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7400830.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IVO69_002067"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IVO69_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7400831.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IVO69_002068"
     CDS             complement(12784..13719)
                     /locus_tag="IVO69_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7400832.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3807 a         3095 c         2435 g         4470 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACCPY010000078        13646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCPY010000078 DACCPY010000000
VERSION     DACCPY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.080"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(179..3457)
                     /locus_tag="IVO69_002069"
     CDS             complement(179..3457)
                     /locus_tag="IVO69_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7400833.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3728..4273)
                     /locus_tag="IVO69_002070"
     CDS             complement(3728..4273)
                     /locus_tag="IVO69_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7400834.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4285..4977)
                     /locus_tag="IVO69_002071"
     CDS             complement(4285..4977)
                     /locus_tag="IVO69_002071"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7400835.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(4996..5280)
                     /locus_tag="IVO69_002072"
     CDS             complement(4996..5280)
                     /locus_tag="IVO69_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400836.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5293..5856)
                     /locus_tag="IVO69_002073"
     CDS             complement(5293..5856)
                     /locus_tag="IVO69_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7400837.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6084..6746
                     /locus_tag="IVO69_002074"
     CDS             6084..6746
                     /locus_tag="IVO69_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7400838.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6870..8066
                     /locus_tag="IVO69_002075"
     CDS             6870..8066
                     /locus_tag="IVO69_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7400839.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8167..9543)
                     /locus_tag="IVO69_002076"
     CDS             complement(8167..9543)
                     /locus_tag="IVO69_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400840.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9580..10446)
                     /locus_tag="IVO69_002077"
     CDS             complement(9580..10446)
                     /locus_tag="IVO69_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7400841.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10535..11560)
                     /locus_tag="IVO69_002078"
     CDS             complement(10535..11560)
                     /locus_tag="IVO69_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7400842.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11578..12252)
                     /locus_tag="IVO69_002079"
     CDS             complement(11578..12252)
                     /locus_tag="IVO69_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400843.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12433..13329)
                     /locus_tag="IVO69_002080"
     CDS             complement(12433..13329)
                     /locus_tag="IVO69_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7400844.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(13403..13528)
                     /locus_tag="IVO69_002081"
     CDS             complement(13403..13528)
                     /locus_tag="IVO69_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7400845.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         3977 a         2945 c         2400 g         4324 t
ORIGIN      
        1 gtggagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaagca
       61 gcaaggagaa cctcatctat aaaaagatag aaggaagtga gacatctatt ggtctcattt
      121 ccttctattt ttactttcct cagtcaattg ttttacatca taaaaggaga aattccactt
      181 atttttttcg ctgtttcatg acataaacaa ctacaccgat taacaaaatt cctccagcta
      241 atgctagcaa cgggttatta aattctcccg tttttgggag tccttgagtg ccattttttt
      301 cactttcttt tgctgtccta tccggatgag aaggttttcc atttggtttt ccatttggtg
      361 attcatttcc tggattttct ctttctggtt tgtcatttcc cgggggcaca tcggcagttt
      421 ctcgcacagt gatcgctttt tgctgggtaa cagattctgt atgagcagac catttttccc
      481 catcaaatat ttccgaacga taagtgactt ctagcgtata ttctcctttt gaaggtgctg
      541 aaaaaatagc ggtatgatta gaaaattgag cacttgactg tgtttcaacg atttggatct
      601 tttctgggaa ataacgcata tcttcaaaga caggcgttcc ttgatttggt gtgaaagttg
      661 acacaaaaac tgtaaaaggc ttcagttcaa tgatatttcc ttctgcattc tccgccacac
      721 catttgcttc ttgtataata atcggattat tttgttcatt tggttttgtc tgatttggtt
      781 cttgtacgat tgcatactct ggatattccg aagacggtga ggtttctccg tcaaaaatag
      841 cacctacaga taatggaaga ccatccactc tttccttttc tgtttgacga ttccccggtt
      901 taatctgcat cagtccatga acatggatac ttccattttc atagcgcgta acaggctgct
      961 gaccttcttt cgaacatgta tttagagact cgatccaatc ttttgatatg gtatgtccaa
     1021 gagtattttc tgattgtttc tctcgttcat tccaatagta gttcaacgag tggtagattt
     1081 gatcttcgtt ttgattcaat gggcggatct gctctctcat ctcaagattc tctgttcgga
     1141 aagataagat accagaagca tggtagtctg tgtttcctgc tgaagaggta taaagagcca
     1201 cattatatga accgttatta aaacttgtac tgctattgac gataatatct gttccgctat
     1261 tggaatcgat tcctttcgca agattttcga aagacaaact attgatcaat tcatgagggc
     1321 ccgttaaaga agaaccgccc attttgaatc cattaccgtt accttctccc tcaattcctg
     1381 gtacccagcc gtttcgataa gcaactgagt ttttgatgat tacttttccg atcgaacctg
     1441 tttcattttt agcaaaaagg tcccaaccgt catctgcatt atgatacgcg atacaaccgt
     1501 caaatacatt tccttctcct attgtcagtt tggccgcaaa tccatcggca tcttcaaatc
     1561 ctggatcggc attttcaaaa gatgtacagt tcttgactaa gttatgcgcg ggccatgctt
     1621 cgaaaggatc atttgagcta ccgctgattt ggattcctgt atttccattc cgataagcat
     1681 gaatctcttc gatcaaatta tgatttccag aaatctgtaa tcctttttgc atagctgcac
     1741 tatttgtaat gtcaaaccct cgtaaacccc agtagtgact gaacagtgtc atcccatttc
     1801 cagttccttt aaaatcaagt acaggacgct tgttttctgg atttttctct gcaatcaact
     1861 gaatcgggtt ttctcttgtc ccgttaacgc cttttggaat cgtcaatcct ctttcgaagg
     1921 aataagttcc aggcgccatg acgattgtct gcccagcata tgcgtatctc aatgcttgtg
     1981 tcagagataa tggattttgt ctgcttccgt ttcctgcatc tgacccttct ggtgttacat
     2041 aaactgtcga atatttaggt ttacggtagt ccactgtatg aagaatctct tttgtttcat
     2101 aactagtcat ctctacgtat tcttctggcg gatagttttt atctggcgta aaactgaaag
     2161 taaattcgtt cgttccttcc atcagttttg tctgataatc aaattcccga ttcgcagtga
     2221 tagccaagtt ttttgcgttc ttaagcacct ctcctgttgc tttattttta attgtcaata
     2281 atccgtcagc atttgctcta aaggacatag tatgatcttg gtttcctgaa tgattactag
     2341 aggtcacagt gtaaatcgga tcaatcaagc gagtaggtct ttcttgccca ggaggatctg
     2401 tcgcaggatc tgagtaagca acttcgattc cttctacctt gatcgtcata ttccttgctg
     2461 cagcaaatcc tgcataaata accgattcat ctgaagcgta taatttttcc cagtcataca
     2521 tgatgtcaga agccatttct tctcctgttt caggatcgta ataatatgcc tctaaaccag
     2581 tattcgtttt tttcatctct acgtccagtt tagtaatcga gttcgtggcc ggaaacagat
     2641 tcccatgacc gtttccgaat atattataag aacctgtttc tttttccaag aaccctgctg
     2701 aagtctctaa aggtgttgtt tccactgaga cactcccagg agctggcgca gcttgcggat
     2761 cttcagatga tattcctgtg attgcccgtg tccccaatcc aagtcgcatc gtatatttgc
     2821 tgccgctcgt gttgaccact tcttgtctat ccgagtccca gacatattcg atccgactgc
     2881 ctagtatggc aaatgaattt gaataaaatg cctgcccaaa caaatgattt tttggtactg
     2941 cgtctcttac cattaaagca aatccttctt gggcattcgt ataggtccaa gattctattt
     3001 cgatcgtcgc ccgcatccta aagttgatgt ctttggggat tgcttgataa taataggtca
     3061 ttccatctga tccgcttgac tggaattttc cgccgttatt cgtggaagag agccgcacgc
     3121 cgtcttctaa tggttcaatg gcatacctac tagcacttgt gctttctccg aaaacaaccc
     3181 ctttccactc ttcatttgct tcttcagatt gcttttttgt cttttcattt tctgattctt
     3241 tcatcatagg atcatttcct tcttccgtat ccatatcttc actagttctt ggtatttcct
     3301 ccattgttga catcccttca atgttcacgt gtgcattttc ttcgttcaca gactgttgtt
     3361 cgttagcagc aacttcaacg acagaaatta aaaccggggt actgagcata aggagattca
     3421 tcattagcgc actgtatttt atacgttttt ttaacaagtg tattcctccc gtaacaaatt
     3481 agaaagcgtt tgcttaaagt attacctaat ttatttttac tgtcaataaa aaacaaaatt
     3541 ataataagtg tattttataa aaagaaaaaa tataacttaa gttgttgata ataaagagat
     3601 ttattaacgg aaaaagaata agttttttta aaataacgta taaaaaagag cataaaacaa
     3661 agtcgtttgt tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt
     3721 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
     3781 gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat
     3841 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
     3901 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
     3961 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
     4021 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
     4081 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
     4141 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
     4201 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
     4261 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
     4321 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
     4381 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
     4441 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
     4501 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
     4561 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
     4621 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
     4681 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     4741 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     4801 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     4861 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     4921 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     4981 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     5041 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     5101 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     5161 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     5221 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     5281 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     5341 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     5401 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     5461 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     5521 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     5581 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     5641 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     5701 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     5761 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     5821 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     5881 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     5941 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     6001 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     6061 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     6121 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     6181 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     6241 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     6301 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     6361 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     6421 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     6481 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     6541 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     6601 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     6661 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     6721 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     6781 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     6841 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     6901 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     6961 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     7021 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     7081 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     7141 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     7201 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     7261 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     7321 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     7381 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     7441 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     7501 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     7561 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     7621 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     7681 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     7741 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     7801 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     7861 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     7921 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     7981 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     8041 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     8101 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     8161 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     8221 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     8281 tacaatacaa gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     8341 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     8401 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     8461 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     8521 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     8581 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     8641 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     8701 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     8761 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     8821 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     8881 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     8941 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     9001 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     9061 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     9121 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     9181 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     9241 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     9301 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     9361 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     9421 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     9481 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     9541 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     9601 taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt
     9661 tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat
     9721 ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat
     9781 gtttcctaag ccccataata atccacagat catgtttttc cacacaaact gatcgacttt
     9841 aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat
     9901 aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat
     9961 cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc
    10021 tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa
    10081 agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc
    10141 cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc
    10201 catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa
    10261 aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa
    10321 agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat
    10381 tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat
    10441 ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac
    10501 aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta
    10561 agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat
    10621 ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg
    10681 ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt
    10741 ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgt gcttcctttt
    10801 ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt
    10861 ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacaa
    10921 tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa
    10981 tgattccttt tagcctatat tcttggatga ggtagtcccg cgaaaaactg tctagtcttg
    11041 cataatacaa aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg
    11101 tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa
    11161 gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac
    11221 tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta
    11281 ccagtttttc ttcttctgtt cgtccaccgc tcaaatagtt ttgcaaaggg gaagtttttt
    11341 gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa
    11401 aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga
    11461 tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga
    11521 tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca
    11581 gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc
    11641 gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc
    11701 gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat
    11761 caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc
    11821 catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa
    11881 ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat
    11941 ttggaaaggt tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat
    12001 attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg
    12061 acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc
    12121 ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg
    12181 ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat
    12241 gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc
    12301 cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg
    12361 gaaatatgcg atttaatcgc ttttgtcttt tcaatacagc ttaaatttat tatgttattc
    12421 ttaacaattg gtctatgatc tgttattttt ccttagttgt ttttgtcttt taaatttcca
    12481 ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa
    12541 tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg
    12601 aactcgcctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat
    12661 aagtgtgact agatcctgct tttctctgat tgctaatgta tttgtttttg ttggaaggac
    12721 aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg
    12781 gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg
    12841 ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac
    12901 tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt
    12961 gattttcgga atatagattg ctccgatcat atgttgttcg tagtattctt ttcctggatt
    13021 tcggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac
    13081 tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt
    13141 ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata
    13201 attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat
    13261 cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat tacctgttct
    13321 ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaatcggtt ttataaaggt
    13381 tgctgcagct tatttttgtt gcttatactt cagacttcat tttaaattta cggtaccagc
    13441 tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac
    13501 cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta
    13561 ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg
    13621 ctgcctcacc tgttggaagt acataa
//
LOCUS       DACCPY010000079        13521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCPY010000079 DACCPY010000000
VERSION     DACCPY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.081"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(102..626)
                     /locus_tag="IVO69_002082"
     CDS             complement(102..626)
                     /locus_tag="IVO69_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400846.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IVO69_002083"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IVO69_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7400847.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IVO69_002084"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IVO69_002084"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7400848.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="IVO69_002085"
     CDS             complement(2348..3028)
                     /locus_tag="IVO69_002085"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7400849.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="IVO69_002086"
     CDS             3154..4023
                     /locus_tag="IVO69_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7400850.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="IVO69_002087"
     CDS             4049..4564
                     /locus_tag="IVO69_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400851.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="IVO69_002088"
     CDS             complement(4611..5174)
                     /locus_tag="IVO69_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400852.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="IVO69_002089"
     CDS             complement(5229..5408)
                     /locus_tag="IVO69_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400853.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="IVO69_002090"
     CDS             complement(5485..5994)
                     /locus_tag="IVO69_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400854.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="IVO69_002091"
     CDS             complement(6090..6470)
                     /locus_tag="IVO69_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7400855.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="IVO69_002092"
     CDS             6800..7549
                     /locus_tag="IVO69_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7400856.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7733..9010)
                     /locus_tag="IVO69_002093"
     CDS             complement(7733..9010)
                     /locus_tag="IVO69_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400857.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9089..9733)
                     /gene="pcp"
                     /locus_tag="IVO69_002094"
     CDS             complement(9089..9733)
                     /gene="pcp"
                     /locus_tag="IVO69_002094"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7400858.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9932..10876)
                     /locus_tag="IVO69_002095"
     CDS             complement(9932..10876)
                     /locus_tag="IVO69_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7400859.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10878..11564)
                     /locus_tag="IVO69_002096"
     CDS             complement(10878..11564)
                     /locus_tag="IVO69_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7400860.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12038..12358
                     /locus_tag="IVO69_002097"
     CDS             12038..12358
                     /locus_tag="IVO69_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7400861.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12512..13417
                     /locus_tag="IVO69_002098"
     CDS             12512..13417
                     /locus_tag="IVO69_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7400862.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13414..>13521
                     /locus_tag="IVO69_002099"
     CDS             13414..>13521
                     /locus_tag="IVO69_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400863.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLA"
BASE COUNT         3919 a         2788 c         2408 g         4406 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc
     7561 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaat
     7621 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     7681 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctatt ttttatagta
     7741 attccttcag atactttctt gcctcatcat tgaaacgcag tgattcttct aacttctgat
     7801 aatattccaa cgcttgtttc tgagcttcag taaaacgata atgttttcct ttccatttgt
     7861 cgattgtctc ttgcacattt ccagattgga tatctttcac tactagttgc catttttctt
     7921 ttgagactgt acggtctttc ttatcagcag attgtatagc ttctcgttct ttacgcaata
     7981 cttttaaaaa accatttctg actatccgag gaatgctgtc gaagaaccct tctgtaaaac
     8041 catagcgaca atattgcttc caaataaata gatagatttt tggttggatg aatcgttggt
     8101 ttttcgcaaa atattccatc tgagcagtga cttctggtgt tcgttgacgt tttgtctttc
     8161 tcaacacgga aataaatgtt tcaaaataag cattaggatg tactggcact tcttcatata
     8221 cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat
     8281 cctttactaa ttcttctgca aagtcgtcaa atagttcttt ttttatctgt cgcttttttg
     8341 actgcccatt gatgtgttca cttattttct tgatttcatt ttccaatgct tgataatccg
     8401 ctatgtaaat cggcatttct acttggcgaa attctgcgag ccgctgagct aattttatat
     8461 ccggtaaata tggctgtttc atccgacgat tttggtacaa gacaaaacag tatttttgcc
     8521 atagtccttc aggaaaatcg atattcttct gcttaagcca taataattcg tctaaggcca
     8581 gatcaacttt tgtcattccc tgatggatcg aagctgccac tgtcggagag acatgcaggt
     8641 gatcggcaaa gtggttgatg cctagtttta ggatctttcc ggtttttggc gatttaacga
     8701 tgtattgcac ttttaattct ctaccacact cacaatacaa tttttgctgg cttcgttttg
     8761 ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca atctgttgca gctaaataat
     8821 gttcttccat aaaacgagag ctgattagat atcggcggtg cttatcgact agttcccgct
     8881 gttttttcgt taattttgca tagatctccc aacgttgact gcttgtgtat tgtttagaaa
     8941 ttttgatggg atagcttttg ctgatttctt ttggaaacca ttcttcaaag acggattgcc
     9001 attcttccac ggctgtttct ttcctttcta attacaaaaa gctggctact aacataacgt
     9061 tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt tccggcattt ccggaaatat
     9121 cttctttatt ttctactgct gcttcaactg cataagtcaa agaagaagca atgtcttcta
     9181 aagacataga aggcgtaccc actggacgat cgatcacttg atcgggtgag aatggcacgt
     9241 ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat caaatgaagc aaacgataca
     9301 tgatatcatt gcagacaaat gttcctgctg tataagagat gtacgctggt agaccatgat
     9361 cttgtacatt ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg gctggaccgt
     9421 tttcttctaa agccgtatcg aatggctgat ttccttcatt atcagggatt cttgcttctg
     9481 ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct tgtccgacac
     9541 agatcacgac atctggttgg tgctgttgga tggcttcttt gactacttga ctgctttttt
     9601 cgaatacggt cggtacttcc actttgatga tttcagcacc gctgatttca tctggcattt
     9661 tttttactgc ttcataggct ggattcactt tgtccccacc aaatggatca aaacctgtta
     9721 ctagtacttt catctgttgt tcctccgtcc gattgattta acatatggtt atcttttatc
     9781 ataccatttt ccttgaacag cgtaaataaa ggaaacaata gtttaaataa cataacagat
     9841 caaacttaat atattcttat aaaaaaatag tgttccacta caatgttaga agaggttgcg
     9901 acaattattc tgtcacaacc cctctcttct attatttaaa tagaatcata taagcaattt
     9961 ggaaaactag cataaataac gcaataaaca actggttttt gatcactccg tatttgtctt
    10021 tcatctccag catcgctaca ggcacgatat tgaagttagc agccatcggt gtcagcaggg
    10081 tcccacaaaa accgcaagtc aatgcaacca ttcctattaa cgctggattc gctccatgac
    10141 taaatacgaa tggtgcaccg atcccaaccg tcatcacggt gatggccgca aatgcattcc
    10201 ccatgattac cgtaaatatc accatgccaa gagcgtaaat gatgatgccg atgttcacat
    10261 ttccttctgg aacgatcgta cctactgcat tggaaatcac tgtcccaact ccagcgcttg
    10321 taaataccgc acctagagaa gctaaaagca ttggcaacat gcttaacggc cctactgtgc
    10381 ccagcatatc tgctgcatca tctaaaaatg tacttggtcg attatctcga gaaaagaaca
    10441 tcaagattag catggctgca aaaacaccga cgcccacgcc gactaaagca cctaaattgg
    10501 taaatagagc aaacaagatc gcaaagacac cgatactcaa agctggaata aaaattttca
    10561 ttcctagttt gtctgacatt ttttccatat attcttttga tggaagccgg ctttctcctt
    10621 tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga
    10681 ttgccggaag ccatcttcct cctgcaataa ctacccctaa gaccgtccag aaaaaggctg
    10741 acccataacg atgaggattg gttttatctg ttaaatttct tactccagtg taaatcaata
    10801 cgagtcccat cacgatataa aggatctcca gtaatttatc gcctaataaa atatcaggat
    10861 ttgtaaaaaa gtccatctta ttgccccttt cctggataat atttcttcgc taatttcttt
    10921 tctttaagat agaagtagac acttgccaca actaaagccg tgatagccac tggaacttcc
    10981 actaaagcaa gctgtcccaa tgttacgtca tatcctagat ctttcagtgt tgattggact
    11041 agcaacgccc ctgctcctcc gatgaatagt acttggccaa agaaccagca gacattttcc
    11101 atagctgaag ccataccttt tagttcttct tcatgcttag gatggacttc taaccctttt
    11161 gattcgatgg tccccaatgc catcggtaaa atgattggac gaacaaatcc agcaactcca
    11221 ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga tcccataaat atctacaatc
    11281 ataccagcag atactttctt gaatcgcttg atcaaggaag ccgctgattc tttcagacca
    11341 tttctttcaa gcgttcctgt tgccaacata atgatgataa aaatcgccat cccgcgattg
    11401 tttacaaaac ttgtacctaa aagttctaac attccttgaa tgccaagacc gccagtcaaa
    11461 gctgtaacaa ctaaagcaac cattacgatc aatatcgagt ctaatttaaa tgcaaacccg
    11521 agaataacaa tcaagacgcc taaaaggcta agataatttt ccatatattt tcctccataa
    11581 aatgacaata tttttttaat tataaagcaa aacacataaa aaatatagtt gttttctcac
    11641 gaaatagaca ctatttttaa atatttttcg ttattttttt atcacaaaaa ttatcttact
    11701 tattgtaagg taaagctttt tttaatttat tgcccgataa tatatagaaa taaggtctag
    11761 ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat tgcaaagcat
    11821 tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta ctaaaaagta
    11881 tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata caatctgcca
    11941 ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga ttttcctctt
    12001 tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg aattaatcat
    12061 agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg gtttttctaa
    12121 aatcaattat tctttttaca cagtgattgg catgattgct agtttttact ttttgtcaaa
    12181 ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga ttggcgcagt
    12241 tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt ccacttggtt
    12301 ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg gccactagac
    12361 acttggaata agtactgatc agaaaaaata agccttttta tttaatattc aattttttta
    12421 tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca ggtgtatgct
    12481 tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc aagaattatc
    12541 caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat cccctggcaa
    12601 aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga aagctatttt
    12661 aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga tttctccctc
    12721 tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta tctatccatt
    12781 cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat ctgctatcga
    12841 cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga aatcctactc
    12901 gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc cgtctatcgt
    12961 tattttagat gagccgatga acggtttgga tccacaagca acaagagatg tgcgaaaaat
    13021 cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact tactcagcga
    13081 gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca tcaaacaatg
    13141 tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta caacaaatga
    13201 tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc ctgataaaat
    13261 caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat tgtctgcaca
    13321 aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt ctctattgga
    13381 aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa acaagaatta
    13441 tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt tgttttgctc
    13501 ataggaacag cacttctagc c
//
LOCUS       DACCPY010000080        13493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCPY010000080 DACCPY010000000
VERSION     DACCPY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.082"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..523)
                     /locus_tag="IVO69_002100"
     CDS             complement(<1..523)
                     /locus_tag="IVO69_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7400864.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGR"
     gene            complement(670..1353)
                     /locus_tag="IVO69_002101"
     CDS             complement(670..1353)
                     /locus_tag="IVO69_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7400865.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1462..1920)
                     /locus_tag="IVO69_002102"
     CDS             complement(1462..1920)
                     /locus_tag="IVO69_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7400866.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2004..3767)
                     /locus_tag="IVO69_002103"
     CDS             complement(2004..3767)
                     /locus_tag="IVO69_002103"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7400867.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(3988..5448)
                     /locus_tag="IVO69_002104"
     CDS             complement(3988..5448)
                     /locus_tag="IVO69_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400868.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5656..6582)
                     /gene="glsA"
                     /locus_tag="IVO69_002105"
     CDS             complement(5656..6582)
                     /gene="glsA"
                     /locus_tag="IVO69_002105"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7400869.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6605..8032)
                     /locus_tag="IVO69_002106"
     CDS             complement(6605..8032)
                     /locus_tag="IVO69_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7400870.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8063..9277)
                     /locus_tag="IVO69_002107"
     CDS             complement(8063..9277)
                     /locus_tag="IVO69_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7400871.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9618..9860)
                     /locus_tag="IVO69_002108"
     CDS             complement(9618..9860)
                     /locus_tag="IVO69_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7400872.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(9892..10449)
                     /locus_tag="IVO69_002109"
     CDS             complement(9892..10449)
                     /locus_tag="IVO69_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7400873.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10462..10650)
                     /locus_tag="IVO69_002110"
     CDS             complement(10462..10650)
                     /locus_tag="IVO69_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7400874.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10663..11229)
                     /gene="amaP"
                     /locus_tag="IVO69_002111"
     CDS             complement(10663..11229)
                     /gene="amaP"
                     /locus_tag="IVO69_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7400875.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            12459..12932
                     /locus_tag="IVO69_002112"
     CDS             12459..12932
                     /locus_tag="IVO69_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7400876.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            12941..13168
                     /locus_tag="IVO69_002113"
     CDS             12941..13168
                     /locus_tag="IVO69_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400877.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         4034 a         2700 c         2184 g         4575 t
ORIGIN      
        1 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
       61 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
      121 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
      181 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
      241 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctctt gtctcaagaa
      301 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
      361 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
      421 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
      481 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
      541 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
      601 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
      661 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
      721 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
      781 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
      841 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
      901 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
      961 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     1021 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     1081 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     1141 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     1201 atcttctgtc gtatttgctc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     1261 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     1321 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     1381 aaatacagct tcatataaaa atgtaaccct tttctattca ggagacaata atgagctatt
     1441 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     1501 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     1561 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     1621 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     1681 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     1741 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     1801 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     1861 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     1921 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     1981 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     2041 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     2101 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     2161 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     2221 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     2281 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     2341 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     2401 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     2461 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     2521 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     2581 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     2641 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     2701 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     2761 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     2821 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     2881 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     2941 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     3001 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     3061 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     3121 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     3181 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     3241 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     3301 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     3361 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     3421 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     3481 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     3541 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     3601 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     3661 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     3721 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     3781 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     3841 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     3901 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     3961 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     4021 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     4081 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     4141 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     4201 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     4261 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     4321 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     4381 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     4441 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     4501 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     4561 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     4621 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     4681 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     4741 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     4801 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     4861 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     4921 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
     4981 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
     5041 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
     5101 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
     5161 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
     5221 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
     5281 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
     5341 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
     5401 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
     5461 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
     5521 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
     5581 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
     5641 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
     5701 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
     5761 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
     5821 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
     5881 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
     5941 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
     6001 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
     6061 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
     6121 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
     6181 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
     6241 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
     6301 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
     6361 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
     6421 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
     6481 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
     6541 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
     6601 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
     6661 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
     6721 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
     6781 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
     6841 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
     6901 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
     6961 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
     7021 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
     7081 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
     7141 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
     7201 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
     7261 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
     7321 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
     7381 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
     7441 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
     7501 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
     7561 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
     7621 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
     7681 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
     7741 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
     7801 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
     7861 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
     7921 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
     7981 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
     8041 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
     8101 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
     8161 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
     8221 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
     8281 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
     8341 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
     8401 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
     8461 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
     8521 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
     8581 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
     8641 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
     8701 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
     8761 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
     8821 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
     8881 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
     8941 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
     9001 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
     9061 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
     9121 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
     9181 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
     9241 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
     9301 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
     9361 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
     9421 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
     9481 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
     9541 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
     9601 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
     9661 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
     9721 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
     9781 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
     9841 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
     9901 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
     9961 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
    10021 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
    10081 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
    10141 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
    10201 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
    10261 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
    10321 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
    10381 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
    10441 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
    10501 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
    10561 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
    10621 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
    10681 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
    10741 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
    10801 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
    10861 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
    10921 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
    10981 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
    11041 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
    11101 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
    11161 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
    11221 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
    11281 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
    11341 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
    11401 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
    11461 caaagattat gctcagttat tattacaaga ttctatttat ttattaaatt ttttcggaga
    11521 attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat
    11581 tatcattttt cttattcact tctttggttt atactattct tataaataaa aatgaggtat
    11641 gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat
    11701 caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact actacattat
    11761 gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa
    11821 catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat
    11881 ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc
    11941 aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa
    12001 tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt
    12061 acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat
    12121 taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct gttaacttac
    12181 acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc
    12241 atctaaacta gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt
    12301 ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct atcatttttt
    12361 ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa taattattct
    12421 gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa acattgctat
    12481 taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta cttttttata
    12541 ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca ttatttgttt
    12601 ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta ttgaaactat
    12661 tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata ctaaaaaaaa
    12721 tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct aaatttatga
    12781 aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct gtcgtattca
    12841 ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa catgcgattt
    12901 tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta tcgttaattt
    12961 agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat
    13021 tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc gtttttccac
    13081 tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga cctataaaaa
    13141 tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa ataaaaaata
    13201 atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat tcagattttt
    13261 tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca tacaaagggg
    13321 gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct gttacgttgt
    13381 agttggatct gcttttagta taatacttta ttcattccac cagactgata gcagtttgag
    13441 cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga agt
//
LOCUS       DACCPY010000081        13345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCPY010000081 DACCPY010000000
VERSION     DACCPY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.083"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IVO69_002114"
     CDS             complement(45..1364)
                     /locus_tag="IVO69_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7400878.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IVO69_002115"
     CDS             complement(1361..2014)
                     /locus_tag="IVO69_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400879.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IVO69_002116"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IVO69_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IVO69_002117"
     CDS             complement(2724..3440)
                     /locus_tag="IVO69_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7400880.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="IVO69_002118"
     CDS             complement(3490..3699)
                     /locus_tag="IVO69_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400881.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="IVO69_002119"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IVO69_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IVO69_002120"
     CDS             complement(5356..6387)
                     /locus_tag="IVO69_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7400882.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IVO69_002121"
     CDS             complement(6394..7224)
                     /locus_tag="IVO69_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7400883.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IVO69_002122"
     CDS             complement(7221..8027)
                     /locus_tag="IVO69_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7400884.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IVO69_002123"
     CDS             complement(8033..8857)
                     /locus_tag="IVO69_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7400885.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IVO69_002124"
     CDS             complement(8847..9947)
                     /locus_tag="IVO69_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7400886.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IVO69_002125"
     CDS             10125..10910
                     /locus_tag="IVO69_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7400887.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IVO69_002126"
     CDS             10943..11326
                     /locus_tag="IVO69_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7400888.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IVO69_002127"
     CDS             11402..11533
                     /locus_tag="IVO69_002127"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7400889.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IVO69_002128"
     CDS             11502..11738
                     /locus_tag="IVO69_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7400890.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IVO69_002129"
     CDS             complement(11816..12787)
                     /locus_tag="IVO69_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7400891.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4055 a         2912 c         2306 g         4072 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgtta
//
LOCUS       DACCPY010000082        13171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCPY010000082 DACCPY010000000
VERSION     DACCPY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.084"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVO69_002130"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVO69_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7400892.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVO69_002131"
     CDS             1995..4661
                     /locus_tag="IVO69_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7400893.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVO69_002132"
     CDS             5025..5777
                     /locus_tag="IVO69_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7400894.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVO69_002133"
     CDS             complement(5799..6953)
                     /locus_tag="IVO69_002133"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7400895.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVO69_002134"
     CDS             7138..9579
                     /locus_tag="IVO69_002134"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7400896.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVO69_002135"
     CDS             complement(9909..10613)
                     /locus_tag="IVO69_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400897.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVO69_002136"
     CDS             complement(10800..11045)
                     /locus_tag="IVO69_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400898.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVO69_002137"
     CDS             complement(11062..11913)
                     /locus_tag="IVO69_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7400899.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVO69_002138"
     CDS             12079..13083
                     /locus_tag="IVO69_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7400900.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4203 a         2340 c         2750 g         3878 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca a
//
LOCUS       DACCPY010000083        13145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCPY010000083 DACCPY010000000
VERSION     DACCPY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.085"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IVO69_002139"
     tRNA            complement(77..149)
                     /locus_tag="IVO69_002139"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IVO69_002140"
     CDS             530..1099
                     /locus_tag="IVO69_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7400901.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IVO69_002141"
     CDS             1086..1946
                     /locus_tag="IVO69_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7400902.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IVO69_002142"
     CDS             2136..2840
                     /locus_tag="IVO69_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7400903.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IVO69_002143"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IVO69_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7400904.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IVO69_002144"
     CDS             4677..5993
                     /locus_tag="IVO69_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400905.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IVO69_002145"
     CDS             5994..6863
                     /locus_tag="IVO69_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400906.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IVO69_002146"
     CDS             6918..7727
                     /locus_tag="IVO69_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7400907.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IVO69_002147"
     CDS             complement(7830..8459)
                     /locus_tag="IVO69_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7400908.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IVO69_002148"
     CDS             8629..9921
                     /locus_tag="IVO69_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400909.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IVO69_002149"
     CDS             complement(9997..10659)
                     /locus_tag="IVO69_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7400910.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IVO69_002150"
     CDS             10884..11168
                     /locus_tag="IVO69_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7400911.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IVO69_002151"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IVO69_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7400912.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DACCPY010000084        12731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCPY010000084 DACCPY010000000
VERSION     DACCPY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.086"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(154..597)
                     /locus_tag="IVO69_002152"
     CDS             complement(154..597)
                     /locus_tag="IVO69_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7400913.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(613..1773)
                     /locus_tag="IVO69_002153"
     CDS             complement(613..1773)
                     /locus_tag="IVO69_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7400914.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2280..3980
                     /locus_tag="IVO69_002154"
     CDS             2280..3980
                     /locus_tag="IVO69_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400915.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4082..4915)
                     /locus_tag="IVO69_002155"
     CDS             complement(4082..4915)
                     /locus_tag="IVO69_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7400916.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5219..7384)
                     /locus_tag="IVO69_002156"
     CDS             complement(5219..7384)
                     /locus_tag="IVO69_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400917.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7644..9938
                     /locus_tag="IVO69_002157"
     CDS             7644..9938
                     /locus_tag="IVO69_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7400918.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9931..10596
                     /gene="pgmB"
                     /locus_tag="IVO69_002158"
     CDS             9931..10596
                     /gene="pgmB"
                     /locus_tag="IVO69_002158"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7400919.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10589..11596
                     /locus_tag="IVO69_002159"
     CDS             10589..11596
                     /locus_tag="IVO69_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7400920.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11618..12637
                     /locus_tag="IVO69_002160"
     CDS             11618..12637
                     /locus_tag="IVO69_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400921.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3832 a         2674 c         2278 g         3947 t
ORIGIN      
        1 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcaaaga ttgtaacaac
       61 tgcatattca cacagttaaa ttagtgactt caaaatgaaa taatgctccc agcaaaaaat
      121 taggctggga gcattatttc gttattctgt tcatcaaaaa tcagcgtttt gattccattt
      181 catcacaagt tcctcctcat ttactgcttc atctttttgc tgtgtttgaa ttgtaaaacc
      241 ttgtgaaaga tagaaatgaa tagctgattg atttttttga tagactgtca aagtcagtgt
      301 tttattggtt tcttttattt ttttcattag ttgcgtaccg attctttgtt gccggtattc
      361 ccttttcaca aagattcctg cgataaactg atcaatcacc ccagcaaaac caataatttc
      421 ttgatctatt tctgctaagt aaatcactgc atctggtaaa gctttcctaa cagattctac
      481 ttgattctcc caataagttg atggaatgaa ttcatgagaa tccaagttag catgcagcca
      541 aatcgataga acagattcca gttctttggc agacaatttt gttgttgtgc ggatcatttt
      601 ttctctcctt acttattcat tgattttaaa ataaaaacca tttttttgaa gctcttttac
      661 aagttttcgc ccctcaaaaa agtatatctt ctcttgtttt acttttctaa taccaaaaga
      721 aacagcaact ttaggatcat cagctgaaac gattcgataa gttccaccag gcaattgatc
      781 cagcttgact ttcccatgat ggtcgctttg tactgaaaac ggcacaccat ttctagtaag
      841 tgtttttttc ttatgtttca aaatgaatga actaccattt accggtttcc ctgacgaatc
      901 actaataata aatgaaaaat caagcacttt tttacttaat ctatactgat agattagtcg
      961 tccgataaac actaacagta tgactacagc tattatcgtt cctgcaagaa taaaccattg
     1021 ccgcatggtt ctttcctgat ttccttttcc ttcttctttt tttaccgatt cgttataagg
     1081 aatccgatgt cctgtcacta gcaaacgatg cgtgttgatc atatacggcg tacatgtaag
     1141 caaggtaact aagtcttctc cttctgaaat cgttagaaca gacgtgtcat ctggcagtac
     1201 cacttgtata tttttaactt gataagccaa tttctccccc aaaacagtca aaataaacgt
     1261 gtctccgttt tctaaacgat ctaattcggt aaataaccgt cgattaggta aaccgctgtg
     1321 agcagagatc actgaatgcg tcgaatttcc accaagcggg aaagaggttc cttgcaagac
     1381 gcctgctcca ttttcaagga tcctaccatt gagccgatca taaagtgaaa tatttaattg
     1441 gatcttaggt atattgatac ttccgatcag ccattcctct aattggctgg atgttgtact
     1501 ttcatttctg ttcttttcat tgaaaggatc agtaccagga ttgaatcctt ggtcagttaa
     1561 tcgttggttt ttctcctcca ttttctttcg ctgcacttca atttcagctg cggtccgttt
     1621 ttgtacttgt tcgatacgtt tttgatcaat aaaactattt aaagaatcca cataaaatgg
     1681 atataaagcg atcagtaacc ctacgacgat acatattaat aaaaacaaac gaaacaaaag
     1741 tcgacgagac tgtttttgct tactctgctt cacatgtgtc ctcccttgca agtttttttg
     1801 ttctttcttt tattatatgg gttcaggaaa aaaatagaaa atgaaagccg atgaggttgt
     1861 gaaaaagctg tcctgcatca agtaataagt acagcccaaa aaaatagcta ctcatatttt
     1921 ttgttgggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
     1981 tgtgaaaact gcgttatgct ttgagtaata agagcgctag tcagaaaata gcttctcata
     2041 ttttctaggc tagtgagctt attaccgaga agcagctgat tgaacaccgt ttaccgagag
     2101 gtcatgaaaa agctactttg tttcgagtat taagaaaaat tctgtttcaa aacaaggaga
     2161 tgcaacaaaa cttatgtcgc atcctctctc cttagaactt tcacttatgt ttttcttgta
     2221 aaattcataa atattttcat gaattttcta atttcttgcc tacctctcgc ataggatata
     2281 tgatgaattt atctaaagtt tgcaaaggag ttcgacaatt gaaaaaatat ggcccatttt
     2341 tagcaatttt tttcattgct ttggcatttc tcatgcctca tttattcaca cataaactaa
     2401 ttttgggtgc tgatgcccca ttccattaca atcggttcta tgatgccgcg caacaaatta
     2461 aatatgggga tttccattat tttccttccc tttatggatt ccagcaaagt ggcaggatcg
     2521 tgaatgccgt ttacggccca tttttcgctt atttacaagg attgatcgtg ttgcttgcag
     2581 gtagctggtt tcattatcag attctttcaa atttcattct ttatcttatt tcaggatttt
     2641 ctatgctgat tttactgaga tatgtcaatg ttcgaaattg ggtaagtgta ccagtagccg
     2701 ttttattcat gagtacgtat tctattcaat actggtctat caaccaagga ttcactagct
     2761 gggggacttg tttttatcct ttatgtatga tccctattgc tgatttcgtc cttcataaaa
     2821 agttccctgt tatcaaagtc gctgtctcag tgggacttat gacacagatc catttactgt
     2881 cttcgatcat gttagctttg atgtatatcc ctttttatct ttattacttt tttaaccaga
     2941 ctgaaaaagg aaaagcactg cttcaactga ttaagtcggt cttgctttat tttgttctga
     3001 cagcaaatat ttgggcagga ttagctattg tttacagtgg gaataagatt cttcctccct
     3061 ttgttaacag agaaatgagt caaaaggcga tagatctgga tggcagttat tggctccatt
     3121 accctaaggc ctttcctata ttgatattat caggtttttt gctttttatt ttctttcaac
     3181 aaaagaacac cttattccaa aaaatcgtgt tgtacacaag tcttgttttc ttttttcttt
     3241 ctactagctt gattccatgg acgacattga tcgaaaaaaa tgtgcctttt attagtttga
     3301 tccagtttcc atttcgattt ttcgtgccat ttacgctatt gcttctactt taccttgccc
     3361 tttcactgaa ccagtggtct gaacaaaagt ggttccgtct tttaagtata tttggcattc
     3421 ttattttttc agcacagacg atccagaact tataccagca tctggaaaag tgggaaaatg
     3481 aaacatttgt cagtcgacat acttatctat tcgatacgcc ggaagaagca aggaaaacat
     3541 tcttcagcaa agatatgtct accagcttgt tcgttttcca aaaaaccaca cctgactatc
     3601 tgccaatcta taaggagaca aaagacaata aatacgaccg gtataacgaa gaaatactag
     3661 aaaacgaaac aacttttata aaaacacatc caggcggtct tttgacaatc aagtggaaaa
     3721 acactgggga caaaactgtt catatccctg ttatcgtcta cgatcggact gtgctacagc
     3781 aaaatggaaa aacattgact gattatgaag taacagatat cgggacccca atcgtgaagc
     3841 agcaaaaagg aatcaacgag ctcacgcttc attatcaaac gccaatctat ttctatttca
     3901 ttctttctct tactttgatt ggctggttta ccttgctatg tttatttatt tatcatagat
     3961 acaagctttt acgtgcttag gcaaaaggga taaatacatc cgatcaaaaa actctccaaa
     4021 cggtataatc gtttggagag ttttatttta atcagttttt ttatgttata tgttatattt
     4081 tttattcgtt cacatctaat tcgatttcca ccccgtagtg atcactgatg attggactat
     4141 cgatcccatc aaaaatccgt tgatattttt taacctgcat tccttcaggg acgaaaataa
     4201 aatctatacg caatttttct gtattttgtt cccaaccatc tattttcttt tccacagtag
     4261 ctgcttttcc tttttcattc gcgacaacat acgaatcttg aagaagaagg caactttctc
     4321 ttacagtata ataaccttga gagtctactg gattattgaa atcacctagg aaaaaaagtg
     4381 gtacgcgagt ttccaaaaag tagttttcca agtttttcca ctcaaaataa aagccacttt
     4441 ttttatctgt ccaccaagaa aagtggcagc tcgagacaaa aatgtcctga tcatctatct
     4501 ttgttttccc aatcaagatc ttgcgtgtct gataatcttc tttcttttga aattgggaga
     4561 cggtaaggac ctgactttcc aatgtttttt tcgtaagcag tgcatttcct tcttcatatt
     4621 tatcataacc gatgtggctc atttcccaac tccaaaaata ctcttttccg tgttccttta
     4681 aaagctgaac taaacggtag gcaaaattat ctttacggat aggtacttga ttctttaccg
     4741 gacagaactt cattagcttc gcaggttcta cttcttcaga ttccagtaat tggttgattt
     4801 cttgtaaagc aatcacttca taatcttcta acaggatctt atctgctaat tcttgcaatt
     4861 ttttctctgg ttctggctca agccagctat gcgtgttgag tgtcatgaat ttcattggtt
     4921 ttctccttgt cttcatttcg aaacaaaagg gttgtgacaa aatttttgtc acaacctctc
     4981 cagcaaatag tgttctcctg ctaactcctc agtaataagc tcaatggacc aggagctatt
     5041 ttttgtttgg ctgtttttat cacttggtgc agggcagctt tttcacgacc tctccactaa
     5101 acagtattca aactgctgct tctcggtaat aagcccaacc aaacgaaaaa taggaggagc
     5161 tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca acctctcctt
     5221 acattttaat ggagccgatc actttaccag ctgtcactgt acctaattgc ttgatcacta
     5281 cttcttctac tttatcgttt gtaaatacga tcatgatcgt tggatctttt tcttcgtttt
     5341 ggatcacatc aagatccatc tcagctaatt tcgttcctgt tcctacttct tggccttctt
     5401 tgacagcaat ctcaaaagct ggctgattca tttggactgt atcgattccc atatggatca
     5461 aaacttcgat accatctttc gtttccagcc caattgcatg tttgctaggg aaaatacttg
     5521 tcacttttcc agcgatcggc gcaacaactt ctcttgatgt tggaataaca gcaaagccgt
     5581 cacccatcat tttttgtgag aataccggat cttttacttc agataaagga atcacttctc
     5641 cagaagctgc ggaaactagt tcttgttcta tacctaagta ttgcttacta cctgcttgtc
     5701 catttcctgt agaaaccatt tctggctctg gaatctctgc tcctgattgc agcagatctt
     5761 cgatatctga tttcaagaca tctgcttttg gtccataaac tgcttggact ccattgtctt
     5821 tgacgatcaa tcccatcgcc cctgctcgtt tccaggcttc ttcagtccct actttttctc
     5881 gatcatttac acttacgcgt aaccgagtca tacaagcatc gacatctttg atattttctt
     5941 ttcctcccag caaatgaatg atctggacca cttgctgatc tgctgttcct gttccgctgt
     6001 tagatacaac ggtttcttca ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa
     6061 agttgaattt tttgatcaag aaatttgcta agaaatacgt aacaacacca aaagcgatca
     6121 cacacaacac gaagttgata agatctccac ctagacccgc tttgatagct aacggtgtcc
     6181 gagtcagcaa ttcgatattc ccaaatgaat ggacccgcaa tggcaagata tctgccatcg
     6241 caaatgcagc cccttggatc actgaataaa tgacatataa tggcacagca gcaaacataa
     6301 acatgaactc caatggttct gttacacctg ttagaaagac agccaacgct gctgaaaaat
     6361 acattgattt atatcttgct ttcttatcag gatcgacatt acgatacatc gcaaaagcca
     6421 ttcccatcaa gatacctgaa gaaccaatca tttgtcctac tttgaaacga gccggcgtcc
     6481 agttatgcaa cacaaactca tattgcgaca tatcccctgc acctttaaga ttcactaaat
     6541 cagtggccca agccaaccat aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc
     6601 ctgataaaat ctcatacgat ccgccaagct gtgtatagtt gataggaatc gtcagcatat
     6661 gatgcaatcc aaatggcaat agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa
     6721 tcggtgcgct ttcttgtgat tctgcaatcc ataaaccaaa attattgatt ccagcctgta
     6781 cgtttggcca caccacagca agaatcaatg ccacgattgt tgaccataag ataacaacaa
     6841 acggaacaaa gcgctttcca ttgaaaaatg acaaagcatc cggcaatttt ctataattat
     6901 agtatttatt ataagccatt gccccgacaa atccagcgat gatccctacg aatactccca
     6961 tattcaaagc tggtgcttcc aatacacttg taaaaaatcc tttaaccatg atcttcgtac
     7021 cgaacagagt gtgggtaaat gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga
     7081 tagccccagt gatccgattg attaaaataa atgagatccc agctgcaaat gctccgcctg
     7141 ctctttcttt tgcccaactt cctcctattg ctaaagcaaa taaaatatga agattaccaa
     7201 taattgccca accaatattt tcaattactc cgcccgttgt gacaagcggc gtccaatcag
     7261 gattgatcaa gggaattgat ttccctatac tgatcatcaa cccagctgcc ggcataactg
     7321 ctaccacaac cattaaagcc tttccaaatt tttgccaaaa ttcaaaacta aatatctttt
     7381 tcattccgtt tagctccttt acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt
     7441 cttttttgta aatgctttat tttttatttt tctgtctata ccattctata aaacctataa
     7501 tacgtgtgtt cccttctata attttctcct aaaaatttat ttttttaagc ttttttctat
     7561 gaaatatttt cttttgtcta ttgacttgtg caatcggttg cgtttattat gaacgtataa
     7621 aacagctcat taggaggaga aaaatgaaac agatcaaacg actatttgct attgatccat
     7681 ggaaaatcag cacgacacaa ttagataaaa aaaatcttcg actgcaagaa tctttaacaa
     7741 gtattggaaa cggttatatg gggatgcgcg ggaactttga agaaacttac tcaggggacc
     7801 accatcaagg tacttatttg gcgggtgtct ggtatcccga taagacacgt gtcggctggt
     7861 ggaaaaatgg ttatccagaa tactttggaa aagtgatcaa cgccttgaac tttatcgcga
     7921 tagatatttc gatcaacgat cagctgatcg acttggcttc tttggaacca gaagactttt
     7981 actgggaatt agatatgaaa aatggtgtat tatctcgtac attcacaatc actacttcta
     8041 ctaataaagt acgcatttcc ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt
     8101 atatctattt aactatagaa atgcttgaag gtagcggaac agtcaaactc gtatcaaaat
     8161 tggatggaaa tgttcaaaac gaagacagca actatgatga acatttttgg aatgagattg
     8221 atcgtggaac tatagaagaa atcggctatt tgacgactaa aactattcca aacaactttg
     8281 ggattgaaca atttactgtt actgcagcaa tgcgccattt cgttaatgga aaatcagtca
     8341 tccctgacta tcacgaagat actttagcat tacatggttg cttgtctgca gaattatctc
     8401 aaggtgagac atggtcagtg gaaaaagaaa ttatcgttct tactagtaga gatattccag
     8461 aagcgcagca agcagaaaaa gctgtctcag aattaacgaa ccagacaaac aattatatcc
     8521 aagcaaaaga agagcaaaca gcggcttggg cagaacgatg gaagcttgcc gatgtggtca
     8581 tcgaaggaga cgaagaagca caacaaggta ttcgattcaa tttgttccaa ctattctcta
     8641 cttactacgg cgaagatgaa cgattgaata tcggccccaa aggattcaca ggagaaaaat
     8701 acggcggagc cacttactgg gatacagaag cgtatgctgt ccctctttat cttgctttag
     8761 ccaaacctga agtaactaag aatctgctga aatatcggca taatcaactg ccgcaagcaa
     8821 tccacaacgc tcagcaacaa ggattaaaag gcgcgcttta tcctatggtc accttcactg
     8881 gtgtcgaatg ccataacgag tgggaaatca cattcgagga gattcaccga aatggcgcga
     8941 ttgcctatgc aatctacaat tatacgaatt acaccggcga tacggaatat ttgaaaaatg
     9001 aaggactgga agttttagca gaaatcgcac gcttctgggc agaccgtgtc catttttcaa
     9061 aacgagcaaa taaatacatg atccacggtg tgaccggccc aaacgaatat gagaacaata
     9121 tcaataacaa ttggtataca aatacgatcg cagcttgggt cttgcgttac acgcgagaaa
     9181 attatttaac ttatcaaaaa gaagctgcta tcactatcag cgaagaagaa ctagccaaat
     9241 ggaaagatat cgcagagaac atgtacttcc cagttgacga agaattagga atctttgtcc
     9301 agcatgacac attcttagat aaagatctca tgcctgtatc cgatattccc acaagcgatc
     9361 ttcctctgaa tcagcattgg tcttgggaca aaatccttag atcttgcttc atcaagcagg
     9421 cagatgtact acaggggatt tatttcttca atgatacttt tacaaaagaa gaaaagcaaa
     9481 aaaactttga tttctatgaa ccaatgactg tccatgagtc ttccctttcg ccaagtatcc
     9541 attcgatttt agcagcagaa ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa
     9601 cagctcgctt ggatttggat aattacaata acgacacaga agacggcttg cacatcactt
     9661 ccatgactgg tagctggttg tcgattgtcc aaggatttgc tcaaatgaaa actgcccatg
     9721 atcagttgag attcgcacca tttctgccaa aaacttggaa agcttattct ttccatatca
     9781 attatcgcgg acgtttattg ttcatctcag tcacagatga acaagttact ttcgagttat
     9841 tagaaggttc tcctctagaa atttacgtct atgaacagtc ttattcttta gcggatcgtc
     9901 tagaaataac gctaagaaag gaggaagcaa atgtttaaag gggtattgtt tgatttagac
     9961 ggtgtcatta ccgatactgc tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa
    10021 atcggcatta cgatcgacca cacattcaac gaacagctaa aaggcgtcag ccgagaagac
    10081 tcactctctt tgcttttagc ttatggcgga aaagaacact ctttttcaaa ggaagaattt
    10141 gcagaattag cgaaaagaaa aaacgattat tatttagaga tgatccagac gatcgaacca
    10201 aaagatgtct tccccggggt cgtgccttta ctggattctt taaaagaaga aaaaatcaag
    10261 attgctcttg cttctgcaag taaaaacggg ccattcctac ttgaaaaaat gggtcttaca
    10321 ccttactttg atgctattgc cgacccggca gaagcaacaa acggaaaacc tgcgccagat
    10381 atcttcctcc ttgcagcaaa agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa
    10441 gacgcgcaag ccggtatcca agccatcctc tcaagtggcg ctcaaccaat tggcgtggga
    10501 cgtgctgaag accttggaga ggagatcccg cttgtccctg atacgacttt tcttacgatc
    10561 gattacttga aagaaaagtg gcatgaccat ggataatcca gagatctccg tcgtaccagt
    10621 tccagagact gaaataaaga aaatcacatt gaagaacgac tacttagaag tcgttcttct
    10681 caattacggt gcccgcctgc accaaatctt cgcaccagac aaagaaggaa aatcagaaaa
    10741 tgttttattg tcttacgatc gtttcgaaga tgttctaaca gacaaaagct tttttggagc
    10801 aactgtcgga cctgtagccg gaaggatccg ggatgcttct tggggtgagc atagattgga
    10861 gaaaaactgc ggatcacacc atatccacgg tggcacgaaa ggctggtctt tccaattttg
    10921 ggaagtagaa gttttcaaaa ctccatcatc aataggtgtg gtcttttact taaaagatga
    10981 attttctacc tatccaggac caatcactgc tacgatcact taccgtttaa caaaaaacga
    11041 attagagatg acaacctctg ctagtagcct tgttgaaacc atctgcaatc cgaccaacca
    11101 tgcctacttc aacctttcag gaaatggaac aagagacatc tatgaacatc agttaacggt
    11161 ctttttagat ggtgtgcttg aattagatca agaaaaactg cctactggaa attggacaaa
    11221 aaaagaaaaa ctgccaatcg actttagaaa atccccaaca ctgcaagaga tccttgcatg
    11281 ttatccagat ggactagatg atgtatttct tttgcatcat cctaggcttt ccaaaacttc
    11341 cttacaatta ttcgagaagc attctggtcg ccaaatgact atagcaactt ctaataaaag
    11401 tatggtatta ttttctacga ctggattcga agctgatttc tctgtcaatg gaaaacagat
    11461 gcattcaaac tacggattag caatcgaacc gcaagaaatc cctgatatcg ttcatttccc
    11521 caaatttgga tcgatcaacc tccatcctgg tcaagaacgg atcagtcaga cgatttatcg
    11581 gttctctgca caataacaag agaaaggtgt ttttatcatg gctattaccg taaaagatgt
    11641 ggcgaaaaaa gccggtgttg ccacctcgac cgtctctcgt gtcatcaacg atcatcctag
    11701 tatttccgag acaacaaaga agaaagtccg aaaaattatg gatgaactag gttatgttcc
    11761 taacatgact gcccgcaatc taggaaaacg catttccagt gccatcggcg taatcttacc
    11821 ccctcttgat tcaaaagagc ggatcggtaa cccattttat cttgagatca tggaagcaat
    11881 caacgaagaa gcgcggaatt atgatatgac tacagcaatc gctactgcta aaaattttga
    11941 attgctgtta gagaacgtcc agcgtatgca tttacaaaaa caagtagatg gttttattct
    12001 agtgtattca gatagtcaag atactgtcat tgattattta taccaaaacc acatcccttt
    12061 cactttgatc ggacagcctt atcatcatga gaacgaaatc gtttatgtag ataatgataa
    12121 ccagcttcta ggtaaacaag caacagaatt tttgatccag aatggacata agaatatcct
    12181 gtttgtgaca aacacgactc atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa
    12241 agcaatgatg atggctggtc ttcctgcgtt atccagtgta gtactagagc atccggagga
    12301 ttatctgtcc tttgagaaaa cgttgaaaga aaacgaagcc acagcattgg tggtcattga
    12361 cgatatcttc gcccttcgca cgatgcagtt ggcgcaaatg tatggctatg tcattccaga
    12421 gacactctcc gtcattagct tcaacaactc gatcttctca acattaacac acccttacct
    12481 tacaagtatc gatatcgata tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga
    12541 attgatccat gaagaattga gcagtggcgt acgtttggtc atccctcatc gcttgatcaa
    12601 aagagagaca gtcgttcctt tagaaaaccc atactaaata gctgactcac taaatacgct
    12661 gaccatttat cctatggtca gctttttatg taaaagaaga tactacttct aacacattgt
    12721 ttatctgtaa a
//
LOCUS       DACCPY010000085        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCPY010000085 DACCPY010000000
VERSION     DACCPY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.087"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IVO69_002161"
     CDS             6..305
                     /locus_tag="IVO69_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7400922.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVO69_002162"
     CDS             378..1739
                     /locus_tag="IVO69_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7400923.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVO69_002163"
     CDS             1761..2021
                     /locus_tag="IVO69_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400924.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVO69_002164"
     CDS             2035..2787
                     /locus_tag="IVO69_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7400925.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVO69_002165"
     CDS             complement(2881..3486)
                     /locus_tag="IVO69_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7400926.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVO69_002166"
     CDS             complement(3539..4513)
                     /locus_tag="IVO69_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7400927.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVO69_002167"
     CDS             complement(4891..5670)
                     /locus_tag="IVO69_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7400928.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVO69_002168"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVO69_002168"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7400929.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVO69_002169"
     CDS             7161..8558
                     /locus_tag="IVO69_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7400930.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVO69_002170"
     CDS             8639..9079
                     /locus_tag="IVO69_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7400931.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVO69_002171"
     CDS             9227..9553
                     /locus_tag="IVO69_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7400932.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVO69_002172"
     CDS             9554..10303
                     /locus_tag="IVO69_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400933.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVO69_002173"
     CDS             complement(10383..10838)
                     /locus_tag="IVO69_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7400934.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVO69_002174"
     CDS             complement(11265..11540)
                     /locus_tag="IVO69_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400935.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVO69_002175"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVO69_002175"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7400936.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACCPY010000086        12109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCPY010000086 DACCPY010000000
VERSION     DACCPY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.088"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            213..1691
                     /locus_tag="IVO69_002176"
     CDS             213..1691
                     /locus_tag="IVO69_002176"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7400937.1"
                     /translation="MKLKENWSRTDRYRPYKEWSEEHLHSLEKKINSSHWRLGYHIQP
                     VTGLLNDPNGFSYFDGKWQLFYQAYPMGSVHGLKSWYHLSSVNLIDWKNDGLKLIPDN
                     SFDSHGVYSGSALPIDNQLFLAYTGNVRDSNWGRHSFQLGAWMDKNGNITKIPTPLIS
                     SPPPGYTQEIRDPQLIAYDDGYLLIVGAQTEQKQGRILTYYSKDLQNWQFLGTLGFTE
                     EEMGFMIECPNLLFVNGKALLVFCPQGLSHEIHSYSNIYPNTYVIGQTFDFEKNELSD
                     PTHIKNIDEGFDIYATHCFSDENGRTLAISWIGLPEISYPTDEEGWAHCLSIVKELTI
                     EKEQLYQRPVKETAQLRKGKPYHFSGLETTIDAEINQYELQLEFDYSQKGTILLFADE
                     QQKHGLALSFDMIHGKMSMDRSHAGKSFAENFGTVREFSIEKKPLKLQIFVDRSVVEV
                     FVNDGQQTATARVFPTDDQTGLYIKSDREFKGKLWHLRNMNK"
     gene            1704..2687
                     /locus_tag="IVO69_002177"
     CDS             1704..2687
                     /locus_tag="IVO69_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400938.1"
                     /translation="MAVKLTDVAKKAGVSPTTVSRVINNYGSLSQKTIDKVNQAMKEL
                     NYQPNSLARSLQGKNTQLIGLIFPTVANPFFGELVEQLESKLFDLGYRSILCDSANNK
                     EKERNYLNMLAANKVDGIIAGAHNLGIQEYENIELPIVSFDRYLADGTPIVSSDNFRG
                     GYLATENLYLKGARKIAILTGSEESNSPTNDRMNGYLKFMDEQQLEPQIFQFHSTARS
                     TALKNLEIKRILQNEEIDSIFCTDDLTAVLAYNLCQELDIRIPEQLKIIGYDGTKFIQ
                     NYFPHLSTIAQPITDIADILVDLLLQRIQDAEKPLESHYVLPVKLIQGKTT"
     gene            2709..3590
                     /locus_tag="IVO69_002178"
     CDS             2709..3590
                     /locus_tag="IVO69_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7400939.1"
                     /translation="MLYGAIEAGGTKFVCAVGTKDLEVIERIQIPTTTPIETMNNVID
                     FFKKHQEQLAAIGIGSFGPIDIHPNSATYGYITSTPKKAWQNFDFIGYLNKNLKELPI
                     VFTTDVNAAAYGEYVAGNGKGRSTIVYYTIGTGIGGGALQNGTFVEGFSHPEMGHMLV
                     RRHPVDTFKGVCPYHSDCLEGMASGPAIEQRTGMKGQELSVADPSWELEAYYIAQCVY
                     NTTLLFSPDVIIFGGGVMKQAHLRERVQEALVGLMNHYVDYPAFENYVLPPKLGDDAG
                     IIGCLALALEKSEKDIF"
     gene            complement(4342..4905)
                     /locus_tag="IVO69_002179"
     CDS             complement(4342..4905)
                     /locus_tag="IVO69_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7400940.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(4920..5963)
                     /locus_tag="IVO69_002180"
     CDS             complement(4920..5963)
                     /locus_tag="IVO69_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400941.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDKKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMQTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(6096..6500)
                     /locus_tag="IVO69_002181"
     CDS             complement(6096..6500)
                     /locus_tag="IVO69_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7400942.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(6668..7303)
                     /locus_tag="IVO69_002182"
     CDS             complement(6668..7303)
                     /locus_tag="IVO69_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7400943.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLGSMIFGVLTRLE"
     gene            complement(7569..8621)
                     /locus_tag="IVO69_002183"
     CDS             complement(7569..8621)
                     /locus_tag="IVO69_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7400944.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(8634..9572)
                     /locus_tag="IVO69_002184"
     CDS             complement(8634..9572)
                     /locus_tag="IVO69_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7400945.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(10023..10673)
                     /locus_tag="IVO69_002185"
     CDS             complement(10023..10673)
                     /locus_tag="IVO69_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400946.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(10773..11282)
                     /locus_tag="IVO69_002186"
                     /pseudo
     CDS             complement(10773..11282)
                     /locus_tag="IVO69_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
     gene            complement(11645..12028)
                     /locus_tag="IVO69_002187"
     CDS             complement(11645..12028)
                     /locus_tag="IVO69_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7400947.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNKVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         3816 a         2426 c         2016 g         3851 t
ORIGIN      
        1 ccaaaattat tgaagcagta actataaatt tttcttaagc acaatatgcc atgatttaaa
       61 acaattgtca atcgattatc aaattttaaa taaaataatt ttttatatat aaaaaaatag
      121 cttttataag ccatttaatt ttgatattcg tttgacattt taccagaaaa aatatattat
      181 aattccaaaa atacgtttac aaggagtttg tcatgaaact taaagaaaat tggtcacgaa
      241 cggatcgtta tcgtccatat aaagaatggt ctgaagaaca tttgcattcc ttagagaaaa
      301 aaatcaattc ttcccattgg cgcttaggct accatataca accagttact ggtttattga
      361 acgatccgaa tggtttttcc tatttcgacg ggaaatggca gctattttat caagcatatc
      421 cgatggggtc tgttcatggt ctgaaatctt ggtaccatct ctcctcagtt aatctgatag
      481 attggaaaaa cgatggcctc aaactgattc cggataattc atttgacagt cacggtgtgt
      541 actcaggttc agctttgccg attgataatc aactgttcct tgcatatacc ggaaatgtgc
      601 gtgattcgaa ttggggacgt cattccttcc agttaggggc atggatggac aaaaatggta
      661 atatcactaa gataccgact cctttgatca gctccccacc tccgggatat acacaagaaa
      721 tccgtgatcc tcagctgatt gcttatgatg acggttactt gctcattgtc ggagcacaaa
      781 ctgaacaaaa acaaggaaga atcctaactt attacagtaa agatcttcaa aattggcagt
      841 ttttaggaac tttgggtttt acagaggaag aaatgggttt tatgatcgag tgtccaaatc
      901 tattatttgt gaacggcaaa gcactactgg tcttctgccc acaaggttta tctcacgaga
      961 tccactcata cagtaatatt tacccgaata cttacgtgat cggtcaaaca tttgattttg
     1021 aaaaaaatga gctttccgac cctactcaca taaaaaatat agatgaagga tttgacattt
     1081 acgcgacgca ttgtttcagt gatgaaaatg gacgaacctt ggcaatcagt tggattgggt
     1141 tgcctgaaat cagctatcca actgacgaag aaggatgggc acactgcttg agcatcgtaa
     1201 aagaacttac tattgaaaaa gagcagctct atcaacgacc ggtgaaagaa acagcgcagt
     1261 tgcgaaaagg aaaaccttat cactttagcg gtttggaaac cactattgac gcggaaataa
     1321 atcaatatga actccagtta gaattcgatt actcacagaa aggtactatt ttattgtttg
     1381 cagatgaaca acaaaaacac ggtttagcac tatcctttga tatgatacat ggtaaaatga
     1441 gtatggatcg cagccatgcc ggcaaatctt tcgctgaaaa ttttggaact gtacgtgaat
     1501 tctcaattga aaagaaaccg ctgaaattac aaatatttgt tgatcgttcc gttgtagagg
     1561 tgtttgtaaa tgatggacag caaacagcga ctgcccgtgt ctttccgaca gatgatcaaa
     1621 cgggcttgta tataaaaagt gacagggaat ttaaagggaa attatggcat ctgcggaata
     1681 tgaacaaata aaggatgaag aaaatggctg ttaaattgac agatgtggca aaaaaagcag
     1741 gagtctctcc tactactgtc tcgagagtga taaataatta tggttcttta agtcaaaaaa
     1801 caatcgataa agtgaatcaa gcaatgaaag aactcaatta tcagccgaat tcgttagccc
     1861 gttcacttca aggaaaaaac acccaattga tcggcttgat tttcccaaca gtcgccaatc
     1921 cgttttttgg cgaattagtc gaacagttgg aaagtaaatt atttgattta ggctatcgtt
     1981 ctatcttatg tgacagtgct aataataaag aaaaagaacg aaattatctc aacatgttag
     2041 cagcaaataa agtggacggc atcatcgccg gcgctcataa tctcgggata caggagtacg
     2101 aaaatattga gttgccaatc gtctcttttg accgctatct ggcagacgga acaccaatcg
     2161 tcagcagtga taatttccga ggcggttatc tcgcgacaga aaatctctat ctaaaaggag
     2221 ctcgtaaaat cgctattttg acgggctcgg aagaatccaa ctcccccaca aatgaccgaa
     2281 tgaacggcta tttgaaattt atggatgagc agcagctaga accacagatt tttcaattcc
     2341 attcaactgc tcgttccact gctttaaaaa atttagaaat caaacggatt ttgcaaaatg
     2401 aagagattga cagtattttt tgcacagatg atcttacagc tgtattggca tacaatctgt
     2461 gtcaagagct cgatatccgc atccctgagc aattgaaaat cattggatat gacggaacaa
     2521 aatttatcca gaattatttt ccgcatctgt ctactatcgc tcaaccaata acggatattg
     2581 ccgacatcct tgtagacttg ctactgcaaa gaatccaaga tgctgaaaaa ccactggaat
     2641 cacattacgt tttaccagta aagctgattc aaggtaaaac gacttaactg caaaggagca
     2701 aatgttttat gttatatgga gcaattgaag ctggcggaac aaaatttgtc tgcgcagtag
     2761 gtacgaaaga tttagaagta attgaaagaa tacaaatccc tacaaccacg ccgatagaaa
     2821 cgatgaataa cgttattgat ttcttcaaaa aacaccaaga acaactggca gcgattggaa
     2881 taggttcttt tggaccgatt gatattcatc ccaattcagc aacatacgga tatatcacct
     2941 ctacgccaaa aaaagcttgg cagaattttg attttatcgg ttatttaaat aaaaatttaa
     3001 aagaactacc gattgtcttt accaccgatg tcaatgcagc tgcttacgga gaatatgtcg
     3061 cggggaatgg aaaagggcgt tccactattg tttactacac gattggaaca ggtattggtg
     3121 gcggtgcctt gcaaaatggc acttttgtag aaggtttcag ccaccctgaa atgggacata
     3181 tgcttgtgcg ccgacaccct gtggatacct ttaaaggtgt ttgtccttat catagcgatt
     3241 gcctggaagg aatggcttct ggacctgcaa tcgaacaaag aacaggcatg aaaggtcaag
     3301 agttgtctgt cgctgatcct tcttgggaat tagaagctta ttacatcgct caatgtgtct
     3361 ataacacaac tcttctattt tcacctgatg tcatcatctt tggcggaggc gtaatgaaac
     3421 aagcacatct gagagaacga gtccaagaag ctttggttgg tttgatgaat cactatgtag
     3481 actacccagc attcgaaaac tatgtattac ctccaaaatt aggagatgat gctggaatca
     3541 ttgggtgttt agctttagca ttagaaaaat cagaaaaaga tatcttctaa taaaataagc
     3601 acgttgtcac aacaatctca tcaataagca ggcgaagaat ccacttgatg cgatttcttc
     3661 gtctttttat ttactattaa cttatgttct ccaatcggcc tttcaacccg atatcctcgc
     3721 ctatttttct tgcccttaac tctctgtttc ctgtacttaa cagagtagag gtgtcgtgga
     3781 attcttttgt taacttcaaa ttatgatctt ctacaagttc ctacaatcta atcttggttg
     3841 tttttttatt caacttgcaa cgctagcaag ttccagttga ttctcttttc caattaagtt
     3901 ttgtgatatt taaacttcgc cttgacagct tttctaaata tagaaaagaa catttcctta
     3961 tgtctattat gcaacagctc aatttattta atgtatgtta atgattttgt ggaatgtctc
     4021 cttagacatg ctatttctac tatttaatga tcaatcactt gacctactag taagtagaga
     4081 gcatgcccga acacattaca aaaatatcat cacttatgat acggagctgc gtatcatgag
     4141 tgaatgatat taggaaaact tcataaaata aatcttttat acatttccac ttctagatgg
     4201 tcattcatgt tagaagaatc aaactatttt tttactgtcg taataatatt aaaacactag
     4261 aacaaatcaa ttaaattcca taaaatataa ataatagacc tagcatcatc tggtataaaa
     4321 tggatgctag gtccgttata tttactcaaa attgattcta cgaatagatc ttcttatcat
     4381 ttctatttta gtagtcatgc gttgttgttc ttctaaaggt ggtaatggta taagtagttt
     4441 agataaattt gtaccattta catttggttg cccagttccg taagaaattt tttctaataa
     4501 cttccaatat aatggcgagt ctaagaagca gtctacgtat tctgataata atttctcaac
     4561 taacctaatt cgaatcaagt aagaagcaaa aacagattcc tcacttattt ccttaaccaa
     4621 gaaacttttt cccatagttc ctcctgtcct tgctatcaaa atgtcatttt cttccaatct
     4681 taaatcaact agtttagaat tactaatatc acagtaaggc acactggacc aatttacacg
     4741 tccctcttgt atatctgtga ttctcaaaaa cttaacattg ccttgttttt tcgccgaatc
     4801 tgtatagcca tattgaatct tagtagaaat acttcctaaa taaacccata gtcgaacatc
     4861 gctagatgat ttttcttgtc gttctttatg aacaacagtg atcatcttga gttgtccgac
     4921 tattttttag ttgcagcaat ctttttggga cgcttatttg catgaagcaa cgttgctaag
     4981 tctagtaata aaacattggc taacacagga ttggctttct cgcgatacgc tgcggcgcca
     5041 ctttcaaaca atactgctag acgatcttct acaacaacga tttgttccaa atacggtgga
     5101 aatttgattc gttttaggat ccggctagga gagtcccgct cggacactaa gagttccgag
     5161 gtataaggac cataagaacg actggctaag agataatggc cataaaaagc cacaccttga
     5221 atcttatcag atgtttttct gctcattgtc acggttggtt ggattttgtc tttgcggcga
     5281 attgctgttt gcatttcagc tatcgtcggc aatagataga aagtcaactg tccttcacct
     5341 tttagtgtga agttgccaga taccagttga tcattgtttt gagtcaaata cgatgcttgt
     5401 ggaatctctg agagatcagt tgtcagccaa taagcgattg gctgtcccgt ctcttcagat
     5461 gtatctgcta agatatcgat caaacgtaaa gcggctactc gtccagtagc agatcctgtg
     5521 attgtcaacc ataaaagttc ccgtttttta tcatatacta agccccctac atgtggccgt
     5581 ttaggtaaaa tcagcagttt gatcggctgt cctgtttctc tatccagtac atgaataata
     5641 gaatgatgct gatggtcata gcagtaggca gaaataaaca caaaatcttg agaaaaagcc
     5701 aacccttgcg gcgtcatcca ttgacaaacc tcattttcgc cggtttccgg caccaccatt
     5761 cttgtttctg ttaatccagg aatgttatat tgttttgctt cagccttttc ccaagctgct
     5821 tgaacaatag gataccgttt taactttttt tcaatatcct gatgtatccg ttgctgcttc
     5881 caatgcttaa actttaggac atagtaaaca aggaggccaa tgaccagcaa cactaaaatc
     5941 attattgcta cccgctgtgt catttttttg cctccttgtt ctattcgatc aatcgtacat
     6001 ataccgctat taattaccgt aactacatgt ttcttttact agcgtttctt tataggatct
     6061 ttcataatct catttatagt aacattaaga atcccttatt ctactatagt cacttccaca
     6121 tcaatattgc ctcttgttgc tttagagtat gggcaaaatg cgtgggcttc ttctgcgatt
     6181 ttctctgctt ggtccttctc aagtcctggc aaagaaactt ctaaaacaac cccgatttgg
     6241 tagcccccat cttctaattg aaataatgat acccgtgctt tgacttcaga atcagactca
     6301 agattatttt cttccagcat gtgttgaacc gcgccgttga agcaagatgc gtatccagcc
     6361 gcaaaaagtt gttccggatt tgtcccttca ctatggattc ctgggcggtc gatcttcatg
     6421 tgcatacttc cgtttggtgc ctcaacctcg ccatcacggc ctccacgatt gatcattgct
     6481 gtttcgtaca tttttttcat tgagatcatc ctttctttcg tcgttctata ttaagtgtaa
     6541 cgagtatttt ttaatctgac aaaggtaatg cttgcattcg gcgacatcag ttagacaaca
     6601 tcgatgatca gtgatagaag cagtttatct atacaaaaaa agaagggttc tatgatattt
     6661 ggtgttgtta ctcaaggcga gtaaggacac caaatatcat agaacccaaa tagttaaaga
     6721 aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg
     6781 gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat
     6841 aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga
     6901 aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat
     6961 tccaagagct ttcattcgta ttgatactga ataacccagt aaaaatacaa tcacctatcc
     7021 catataaacc aactgttgca acagcaagta ttgcaagtgt ttttgatgtt ccaacaaata
     7081 ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca
     7141 agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt
     7201 gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca ctaatcacac
     7261 ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt
     7321 tagtgattat tcgatacatc caacaatcaa attttttcac attttaacac atatttttat
     7381 tctagttgcc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa
     7441 tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa
     7501 acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt
     7561 aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc
     7621 tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca
     7681 acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg
     7741 ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta
     7801 atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc
     7861 atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa
     7921 gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa
     7981 acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa
     8041 aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta
     8101 ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca
     8161 agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa
     8221 ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata
     8281 atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg
     8341 actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat
     8401 ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact
     8461 gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct
     8521 aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta
     8581 ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt
     8641 acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc
     8701 gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt
     8761 aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc
     8821 gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct
     8881 actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat
     8941 gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg
     9001 ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat
     9061 cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa
     9121 ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct
     9181 gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata
     9241 tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt gattaatgat
     9301 tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca
     9361 acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct
     9421 ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag
     9481 ccatcgatta ataatacttc gttcccactt tggtgaagca ttaacccaaa gcgacttccc
     9541 attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta
     9601 tacttatttg agaatcattt cacaaccttg ttttgttatt cttctcttat ttatcatgtt
     9661 tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa
     9721 tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaacc
     9781 tcactcctct gattataaaa gaggggacat taacctcgtg tatactaaaa ccttccattc
     9841 attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac
     9901 acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact
     9961 tctttcccag attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc
    10021 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
    10081 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
    10141 tccaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
    10201 cttaaaatta tattttcagt gccagacaag tgatttgtat tgagttgata aaaaagatct
    10261 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
    10321 ctatgtgtta catacaaata attgtatgac ctcacttcac ctaatgaatc aattatttct
    10381 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
    10441 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
    10501 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
    10561 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
    10621 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
    10681 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
    10741 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
    10801 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
    10861 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
    10921 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
    10981 ttgttgatat atttgatttc cttgcttttt tacggcgtgt ctactatcgg cttatctttt
    11041 tgcggaccac gtgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
    11101 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
    11161 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
    11221 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
    11281 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
    11341 ttttaggatc aatgataaaa accgcatcac ttatgatacg gagctgggta tcatgagtga
    11401 atgatattta taaatcactg gatattgaag tatattctgt tttctcaact tatttagtca
    11461 gattttttaa tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag
    11521 tttgtagtgc tgcttctacc tctctcatct gttctacaaa ttccaaataa cttgacgcag
    11581 cttgcggaat gaaaaaatga tatgtgaata tttctcctcc tagcccgctt aatatgccta
    11641 ttttttatac ccatgatttg caggaagggg atcggaagta ggttgcagaa agccgccttt
    11701 cataatctgt cggaaaccgg acatcaaaac ggattatttt acttgtcaca tttgtatttt
    11761 cttgtgagga agtgccaaat accttattta cttgagtgac attctcattg tcctgtctga
    11821 tattgcttcg tataaaagta ccagatccac gtctcgtata taacaaacct tcatcaataa
    11881 ggagttgtat agctttgcgg atagtaacac ggcttgtttt aaactctcta gccagacttt
    11941 cttgctcagg taatttagaa ccttcctgat attttccctc aacgatcctc tcttttagag
    12001 ccaaggctat ttctttatat tttggcatat tttcccctca tttcattgat cctacaaaaa
    12061 aattagtata tatacaaatt tttgtaaagt atatatacta ataaataca
//
LOCUS       DACCPY010000087        12087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCPY010000087 DACCPY010000000
VERSION     DACCPY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.089"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            507..821
                     /locus_tag="IVO69_002188"
     CDS             507..821
                     /locus_tag="IVO69_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7400948.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            837..1223
                     /locus_tag="IVO69_002189"
     CDS             837..1223
                     /locus_tag="IVO69_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7400949.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1252..2637
                     /locus_tag="IVO69_002190"
     CDS             1252..2637
                     /locus_tag="IVO69_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7400950.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2640..2792
                     /locus_tag="IVO69_002191"
     CDS             2640..2792
                     /locus_tag="IVO69_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7400951.1"
                     /translation="MLAGVAIATPALTPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2815..4020
                     /locus_tag="IVO69_002192"
     CDS             2815..4020
                     /locus_tag="IVO69_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7400952.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4063..4284
                     /locus_tag="IVO69_002193"
     CDS             4063..4284
                     /locus_tag="IVO69_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400953.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4401..4898
                     /locus_tag="IVO69_002194"
     CDS             4401..4898
                     /locus_tag="IVO69_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7400954.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4873..5379
                     /locus_tag="IVO69_002195"
     CDS             4873..5379
                     /locus_tag="IVO69_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7400955.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5363..7810
                     /locus_tag="IVO69_002196"
                     /pseudo
     CDS             5363..7810
                     /locus_tag="IVO69_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            7813..9990
                     /locus_tag="IVO69_002197"
     CDS             7813..9990
                     /locus_tag="IVO69_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7400956.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAIIKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLSDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSSGRTQADHTRPDGQ
                     EKSTLGKRIGQTIGAVADTKDKIVDSAGNLKEQVKDIPTNARYAVYQGKSKAKENVRD
                     LTSSISQTRADRASGRKEQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQEQS
                     HDGQTAKQSSIQTSRMESQQAKQERPAVKSDSLSLKAERQNRTTQERTVQKPATSTTP
                     ADRASQRPVTKERPSTVQRVQSQNLRNRPPIKSATIKKGSKKP"
     gene            9987..10988
                     /locus_tag="IVO69_002198"
     CDS             9987..10988
                     /locus_tag="IVO69_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7400957.1"
                     /translation="MKLKALVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10985..11917
                     /locus_tag="IVO69_002199"
     CDS             10985..11917
                     /locus_tag="IVO69_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7400958.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
BASE COUNT         3843 a         2281 c         2564 g         3399 t
ORIGIN      
        1 aaggctacca acataacgat cattacttct ctggtaggct atggacttct agcaatcagt
       61 attttgtcta tagattatgg agtaactata ctgcaagtct tagttttggc tatacctatg
      121 tatctatcat tgtttatcat gcatgaaata tataagctat ttgttttatc aaaaatagca
      181 taaaaatcta gttatccgca taaaaactgg acttatcaca ctttatcaag gtcaaaacca
      241 ctcaatttac tactaattta ctacttatga atgagctttg atacgacgat ttatccttga
      301 aaagtgaaga tataaagata cttccaataa aatttgaata tttaataggt agacacttca
      361 aaaaatgagg tgtctatttt tttacccgat tttgaaagga agtgaactta tgaaaacaaa
      421 aaatcaagaa tcaaaaggtc gttccccact ctttaagacc atcaaacatt cattcagcca
      481 ataaaaaaga aaggataggt aaaaatatgg aacttaaatt tgtgattccc aacatggaaa
      541 aaacattcgg caatttagaa tttgctggcg aggataaagt cgttcagcga agaatcaacg
      601 gacggctaac tgtcttatca agaagctata atctctattc tgatgttcaa agagcagatg
      661 atattgtggt ggtgcttcct gctgaagctg gcgaaaaaca tttcggcttt gaggaacgtg
      721 tgaagttagt caatccacgt attaccgcag agggctacaa aatcggcact cgtggtttta
      781 caaattacct tttacatgct gacgacatga taaaagaata aagaaagaga ggaaaaatga
      841 tgagattagc aaatggcatt gtattagata aagacacgac ttttggagaa ttgaaattct
      901 ctgctctacg tcgtgaagtg agaatccaaa atgaagacgg gtcggtttca gatgaaatca
      961 aggaacgtac ctatgactta aaatccaaag gacaaggacg catgattcaa gtaagtattc
     1021 ctgccagcgt gcctttgaaa gagtttgatt ataacgcacg ggtggaactt atcaatccca
     1081 ttgcggacac cgttgctact gccacctatc aaggagcaga tgttgactgg tatatcaagg
     1141 cagacgatat tgtgctgaca aaggattcta gttcattcaa agctcaacca caagcaaaga
     1201 aagaaccgac acaagacaaa tagtcgctag gtagaaagga gactttttcg catgaaacag
     1261 cgtggtaaaa ggattcgccc atctggtaaa gatttagtct ttcattttac gatagcgtca
     1321 ctcctgcctg ttttcctgct ggttgtcgga ctgtttcatg tgaagacaat ccagcagatc
     1381 aactggcagg attttaacct atcacaagca gataagattg acattcccta tttaattatc
     1441 agtttcagtg tcgcaattct tatctgcttg ctggtagcgt ttgtattcaa acgggttcgc
     1501 tatgatacgg ttaaacaact ttaccaccgt caaaaactgg caaagatgat acttgaaaac
     1561 aagtggtatg aatctgaaca ggtcaaaaca gagggtttct ttaaagatag tgctggtcgt
     1621 acaaaggaaa agataaccta cttccctaaa atgtattatc gacttaaaaa tggcttgata
     1681 cagatacggg tggaaatcac gctgggaaaa tatcaagacc aactcttaca cttggaaaag
     1741 aaattagaga gtggcttgta ctgtgagctg acggataaag agttaaagga ttcctatgtg
     1801 gaatatactt tgctctatga caccatagcc agtcgtattt ctattgatga agtagaagct
     1861 aaagatggta aacttcgctt aatgaaaaac gtatggtggg aatatgataa gctccctcat
     1921 atgttgattg ctggtggtac aggtggcggt aaaacttact ttatactgac actgattgaa
     1981 gccttgcttc atacagattc aaaactgtat attcttgacc cgaaaaatgc tgatcttgcg
     2041 gacttaggtt ctgtgatggc aaatgtctac tatagaaaag aagacttgct ttcttgcatt
     2101 gaaacattct atgaagaaat gatgaaacgt agtgaggaaa tgaagcagat gaagaactat
     2161 aagactggca aaaattatgc ttacttaggt ctcccggcac acttcttaat ctttgatgaa
     2221 tacgtcgctt tcatggaaat gctgggaaca aaagaaaaca ccgcagttat gaataagctg
     2281 aaacagattg tcatgttagg tcgtcaagct ggcttctttc taatactggc ttgtcaacgt
     2341 ccagacgcaa aatatttagg cgacggaatc cgtgatcagt ttaatttcag agtggcttta
     2401 ggtcgtatgt ctgaaatggg ctatggcatg atgtttggca gtgacgtaca aaaggatttc
     2461 ttcttaaagc gaatcaaagg tcgtggctat gttgatgtag gaacaagtgt catatcagag
     2521 ttttatactc cccttgtacc aaaaggatat gatttcttgg aggaaattaa aaagttatcc
     2581 aacagcagac agtccacgca ggcgacgtgc gaagcggaag tcgcaggtgt ggactgatct
     2641 tgctggctgg tgtggcaata gccacgccag cacttacccc cccgtatcta acaggggggt
     2701 acaaatcgac aggaaacagt caaaaaaaca ttagaaaatc ctttggttac aagggattta
     2761 caaaatttca gcgtatgtca aatgggcttt aaaagttgac atacgccttt ttgattggag
     2821 ggatttttac tgaatgaaca aacttggtta cagcatttaa aagaaaaacg cttggcttat
     2881 ggactatctc aaaaccgttt agctgttgcg actggtatta caaggcagta tctaagcgat
     2941 attgaaacag gaaaagtcaa gccatcagag gatttacagc agtccctttg ggaagctctg
     3001 gaacgcttca atcccgacgc tccccttgaa atgctgtttg attatgtaag aattcgcttt
     3061 ccgacaacag acgtacagca ggtggtcgaa aacatcttac aactgaaact gtcctatttt
     3121 cttcatgagg actatggttt ctattcttat tcagagcatt atgctttagg cgacatattc
     3181 gtcctttgct cccatgaact ggacaaagga gttctggtgg aattgaaagg tcgtgggtgc
     3241 agacaatttg aaagctatct tctggcacaa caaagaagct ggtatgagtt ctttatggac
     3301 gttttggtgg ctggcggtgt gatgaaacgc cttgaccttg ccattaacga taagacaggg
     3361 attttaaata tccctgtact cactgaaaag tgccaacagg aagaatgtat ctccgtcttc
     3421 cgcagtttta aaagctatcg cagtggcgaa ctggtacgca aagaggaaaa ggaatgtatg
     3481 ggaaacaccc tctatatcgg ttcattacaa agtgaagttt atttctgtat ctatgaaaag
     3541 gactacgagc agtacaagaa aaatgatatt cccattgaag acgcagaagt aaaaaaccgt
     3601 tttgagattc gattgaaaaa tgagcgtgcc tattatgcag tccgtgattt actcgtctat
     3661 gacaatccag agcataccgc ctttaaaatt atcaatcggt atatccgttt tgtagataaa
     3721 gacgattcca aacctcgttc tgattggaaa ctgaatgaag aatgggcttg gtttattggg
     3781 aacaatcgtg aacgattaaa actaaccaca aaaccagagc cttactcctt ccaaaggacg
     3841 ctgaactggc tatctcatca agttgccccg accttaaagg ttgcgattaa acttgatgaa
     3901 atcaaccaga cgcaggttgt aaaagacatt ctcgaccatg cgaaactgac agaccgacac
     3961 aagcagattt tgaagcaaca gtcagtaaaa gaacaggacg tgataacaac aaaaaaataa
     4021 ctcaaataca aattcattga atatagagag gagaacattt ttatgaattt tggacaaaac
     4081 ctttataact ggtttctatc aaacgctcaa tcactggtgc ttttagcaat cgttgtgatt
     4141 ggcttgtatc ttggcttcaa gcgtgagttt agcaaactga ttggcttttt aattattgcg
     4201 attattgcgg ttggcttagt cttcaacgct gctggagtaa aagacatttt actagagcta
     4261 ttcaatcgca ttattggtgc ttaaataaaa ccgttctttt gtggaatata agtggttttc
     4321 ttatgttccg caaaggaatg gtacaccaaa cgaagtgcgg tagggatttt tgaatctcta
     4381 caaagaaagg acgtgaatat atggacgata tgcaagtcta tattgcgaat ttaggcaaat
     4441 acaatgaggg cgaattggtc ggtgcgtggt ttacctttcc cattgacttt gaggaagtca
     4501 aagagaaaat cggcttgaat gatgaatatg aggaatacgc cattcatgac tacgagttac
     4561 cctttacggt tgacgaatac acttccattg gcgaactcaa tcgactatgg gaaatggtat
     4621 cggaattacc cgaagaatta caatcggagc tatctgctct gctcactcat ttttcaagca
     4681 ttgaagaact aagcgaacat caagaggata ttatcattca ttccgattgt gatgatatgt
     4741 atgacgtggc acgctactac attgaagaaa cgggtgcttt aggcgaagta ccagctagtc
     4801 ttcaaaacta tattgattat caagcctatg gtcgggattt agacctttca ggaacgttta
     4861 tctcaaccaa tcatgggatt tttgaaatcg tctattaaat ctgtcggtac attactactg
     4921 gcagattttc tattttacgg ggtggctcaa tcagctaccc ctatttttta tgaaaggatt
     4981 gattacatga agaaaatacg aagctatacc agtatctggt ctgtggaaaa ggtactgtat
     5041 tctatcaatg attttagact tccgtttccc ataaccttta cgcaaatgac atggtttgtc
     5101 gtgtcactct ttgcagtgat gatacttggc aacttgcccc ctctttccat gatagaggga
     5161 gcatttctca aatactttgg gattcctgtg gctttcacat ggtttatgtc tacaaaaact
     5221 tttgatggta aaaagcctta tggatttttg aagtctgtca ttgcttatgc actgcgacca
     5281 aagctgacct atgcaggaaa aaaagtaacg cttggcagaa accagccaca agaagccatt
     5341 acagcagtta ggagtgaatt ttatggcata tccaattaaa tacattgaaa acaatctcgt
     5401 ctggaataaa gacggggaat gttatgctta ctatgagctt gttccttaca attactcatt
     5461 tctaagtcca gaacagaaaa tacaagtgca tgattctttc agacagctta tcgcacaaaa
     5521 tcgtgatggc aaaattcatg ctttacaaat cagtacagaa tccagcatac gttctgcaca
     5581 agagcgttcc aaaaatgaag tcactggcaa gctcaaagcg gttgcctatg acaaaatcga
     5641 ccaacagaca gacgctttaa tatccatgat tggcgaaaat caagtgaact accgtttctt
     5701 tatcggcttt aagttgcttc tcaacgatca ggagttttct atgaaaagtc ttaccgttga
     5761 agcaaaaaat gctttgtctg attttgtcta tgatgtgaac cataagctga tgggcgattt
     5821 tgttagtatg agtaatgatg aaatcctgcg ttttcagaag atggaaaagc tcttagaaaa
     5881 taaaatctct cgtcgtttca aaatccgcag gttagataag gacgacttcg gctatctgat
     5941 tgaacacctt tacggacaga caggcactgc ctatgaagag tatgagtacc atctatcaaa
     6001 gaaaaagctg gataatgaaa cgctgattaa atactatgac ttgattaagc ctactcgctg
     6061 tttggtggaa gaaaaacagt gatatttgaa aatccagcag gaagatgaaa ccgtctatgt
     6121 agcttacttt accattaaca gcattgtcgg agaactggac ttcccgtcct ctgaaatctt
     6181 ctactaccag caacagcaat ttacattccc gattgatacg tcaatgaatg tggaaattgt
     6241 agcgaatcgt aaagccctat ctactgtccg caataaaaag aaagaactga aagacttgga
     6301 taaccacgct tggcaaagtg ataatgaaac cagctccaat gtggcggaag ctctggaaag
     6361 tgtgaatgag ctggaaacca atttagacca aagcaaggaa tctatgtaca agctgtctta
     6421 tgtggtaagg gtatcagcaa atgatcttga cgaactcaaa cgtcgttgta atgaagtgaa
     6481 agatttttat gacgatttaa gcgtaaaact ggtacgacca tttggggata tgctcggctt
     6541 acatgaagaa tttttacctg ccagcaagcg ttatatgaat gattatattc aatacgtgac
     6601 ctctgatttc ctcgctggtt taggttttgg tgctactcaa atgctggggg aaaatgaggg
     6661 gatttatgtt ggctacagct tagatactgg acgcaatgtc tatctgaaac ctgctcttgc
     6721 cagtcaaggg gttaagggtt cagtaaccaa tgcgttagcg tcggcttttg ttggttcgct
     6781 gggtggtggt aaatcctttg cgaataacct tatcgtctat tatgcggtgc tttatggggc
     6841 acaagcagtg attgtagacc caaaagcaga acgtggcaga tggaaagaaa ccttgccaga
     6901 gatttcccat gaaatcaata tcgtcactct gacttctgat gagaaaaaca aaggcttact
     6961 tgacccttat gtgattatga aaaatcccaa agattctgaa tcactggcta ttgatattct
     7021 gacattcctt acggggattt cctctcgtga tggggaacgc ttcccaatcc ttagaaaagc
     7081 cattcgtgca gtaaccaata gtgaagtacg agggttgatg aaagtgattg aggaattacg
     7141 ggttgagaat acgccactaa gtaccagtat agccgaccat atcgaaagtt ttacagacta
     7201 tgactttgca catttattat tcagtaatgg ttatgtggag cagtctatca gcttagaaaa
     7261 acaactgaac attatacagg ttgcggactt ggtacttccc gacaaggaaa cttcctttga
     7321 ggaatatacc actatggagc ttttatccgt tgctatgctg attgtcatta gtacctttgc
     7381 tttagacttt atccatacag accgaagcat tttcaagatt gtagatttag acgaagcatg
     7441 gagcttttta caggtagcac aaggaaaaac actatctatg aagctggttc gggctggtcg
     7501 tgctatgaac gctggggtat atttcgtgac ccaaaataca gacgacctct tagatgaaaa
     7561 actgaaaaat aacctcggct taaaatttgc atttcgttcc actgacctta acgagattaa
     7621 aaagacctta gccttttttg gtgtagaccc agaggacgaa aacaatcaga agcgattgcg
     7681 tgatttggaa aacgggcaat gccttatcag tgatttatat ggtcgtgtcg gtgtgataca
     7741 gttccaccct gtatttgaag aactgctcca tgcctttgat accagaccac ctgtgcgaaa
     7801 agaggtgtaa atgtgaaacc atcaatagta aacagaataa aatcaaactg gacgctgaaa
     7861 cgtctaggta aagtggcaat gacagtggct ttcacacttg tgattgccat ttttctttta
     7921 gccatgctgg gaacggtggt tcaagctgcg ggcttggtag atgatacggt caatgtggca
     7981 aatgaataca gccgataccc acttgaaaac tatcaactgg atttttatgt ggataatagc
     8041 tggggctggc ttccgtggaa ctggtcggac gggattggaa aacaggtcat gtatggacta
     8101 tatgccatta ccaattttat ttggacaatc agtttgtatg tttccaatgc gacaggttac
     8161 ttagtacagg aagcctattc cttagacttc atttccgcta cagcagattc cattggtaag
     8221 aatatgcaga ccttagctgg tgtgagtgca aacggatttt caacagaggg tttctatgtt
     8281 ggattcctct tactcttgat tttggttctt ggggtttatg ttgcctatac gggactgata
     8341 aagagagaaa ccacaaaggc aattcatgcc attatgaatt ttgtgctggt gtttatccta
     8401 tcggcttcct ttattgccta cgctcccgac tacattaaaa aaatcaatga cttttcatca
     8461 gacatcagta atgccagttt atcacttggc acgaagattg tcatgcccca ttccgatagt
     8521 caaggcaagg acagcgtgga cttaatcaga gatagcctgt tttccataca ggttcagcaa
     8581 ccgtggctac tgcttcaata caacagttca gacattgaaa gtattggtgc tgaccgtgtg
     8641 gaaagtctac tttcaaccag tccagacagt aataatggag aagacagaga aaaaattgta
     8701 gcagaagaaa tcgaagacag aaacaatgtg aacatgacga ttacaaaaac tattaaccgt
     8761 ttgggtacag tcttctttct ctttgtattc aatatcggga tttctatatt cgtattccta
     8821 ctaacaggaa tcatgatttt ctcgcaggta ctttttatca tctatgccat gtttctgcct
     8881 gtgagcttta ttttaagcat gattccgtcc tttgatggta tgtcaaaacg agccattatc
     8941 aaactcttta ataccatttt gacacgagct ggaatcacgc tgattattac gacagccttt
     9001 agtatttcaa ccatgctcta taccttatcg gctggttatc cgttcttttt gattgccttt
     9061 ttacagattg tgacctttgc aggaatctac ttcaaactga gcgatttaat gagtatgttt
     9121 tcgcttcaaa gcaacgattc tcaaagtgtg ggaagtcgtg tgatgagaaa acctcgtatg
     9181 cttatgcacg ctcatatgca ccgcctacag cgtaaacttg gacgctccat gacagcttta
     9241 ggtgctggct ctgccattgc cagtgctaca ggaaaacaag gacagtcggg tagttctggt
     9301 aggacacaag cagatcacac ccgtccagac ggacaggaaa aatcaacact tggaaaacgt
     9361 atcggacaaa caatcggagc agttgctgat accaaagata aaatcgttga ttctgctggt
     9421 aatctcaaag aacaggttaa ggatattccg accaatgcaa gatatgcagt gtatcaagga
     9481 aaatccaaag caaaagaaaa tgttcgtgac ctaacttcca gtatttctca aaccagagca
     9541 gacagagcca gcggacgcaa ggaacagcag gaacaaagac gcaaaaccat tgcagagcgt
     9601 cgctctgaaa tggaacaggt caaacagaaa aaacagccag cttcttccgt tcatgaaaga
     9661 ccagctacta agcaagaaca atctcatgat ggacagaccg caaaacaatc ttctattcag
     9721 acttcacgca tggaatctca acaagccaaa caggagcgtc cagcagttaa gtccgattct
     9781 ttaagtttaa aagcggaacg acaaaaccgt actacacaag aaagaacagt tcaaaagcca
     9841 gcaacttcaa ctacaccagc agacagagct tcacaacgtc cagtcacaaa agagcgtccg
     9901 tctactgttc aaagagttca aagccaaaac cttagaaata gaccaccaat aaaatccgcc
     9961 actatcaaga aaggcagtaa gaaaccatga aactgaaagc tttagtgatt ggtggttctg
    10021 ggctattctt gatggtcttc tcactgcttc tgtttgttgc cattttattt tcagatgaac
    10081 aggacagcgg aatttccaat attcattatg gaggtgtgaa tgtttccgca gaagtgctgg
    10141 ctcataagcc tatggtagaa aaatatgcca aagaatatgg cgttgaagaa tatgtcaaca
    10201 tacttcttgc gattatacag gtggaatcgg gcggtactgc ggaagatgtt atgcagtcct
    10261 cggaatccct cggtcttcca cctaattcat tgagtacaga agaatccatt aagcaaggtg
    10321 tgaagtattt cagtgaatta ttagccagta gcgaaaggct cagtgtagat ttagaatcgg
    10381 ttatccagtc ctacaattat ggtggtggtt tcttagggta tgtggctaat cgtggaaata
    10441 aatatacctt tgaactggct caaagtttct caaaagagta ttcaggtggc gaaaaagtgt
    10501 cttaccccaa tcccatagcc atacctatca atgggggctg gcgatacaac tatggcaata
    10561 tgttttatgt gcaactggta acgcagtatc ttgtcacaac agagtttgat gatgatacgg
    10621 tacaagccat catggacgaa gcactgaaat atgagggctg gcgatacgtt tacggtggag
    10681 cttccccgac tacttctttt gattgtagcg gactgacaca atggacgtat ggaaaagctg
    10741 gaattaactt accacgaacc gcacaacagc aatatgatgt gacccagcat atcccactat
    10801 cggaagcaca agctggcgat ttggttttct ttcattctac ctataacgct ggctcttata
    10861 ttactcatgt tgggatatac cttggcaata accgtatgtt tcatgcaggc gacccaatcg
    10921 gttatgccga cttaacaagc ccctactggc aacagcattt agtgggagca ggacgaatca
    10981 aacaatgaga aaggaagatt taatgatgaa atttagaaaa aatcagaata aagaaaaaca
    11041 gataccaaag gaaaagaaac ctcgtgtcta taaggtcaat cctcataaaa aggttgtgat
    11101 tgccttgtgg gtacttttag ggcttagttt cagctttgcg atattcaagc actttacagc
    11161 tatagatact catactattc acgaaacaac tatcatagaa aaggaatacg ttgatactca
    11221 tcatgtagaa aattttgtag agaactttgc gaaagtctac tattcatggg agcaatccga
    11281 taagtccatt gataatcgaa tggaaagtct aaaaggctat ctgacagatg aacttcaagc
    11341 tctcaatgtt gatacagtac gcaaagatat tcctgtatcg tcttctgtaa gaggatttca
    11401 gatatggacg gtagagccaa ctggcgacaa tgagtttaat gtaacctaca gtgtagacca
    11461 gctcattaca gagggagaaa atacaaagac cgtccactct gcttatatag tgagtgtcta
    11521 tgtagatggt tctggaaata tggtactggt taagaatccg accattacca acatacctaa
    11581 gaaatcaagt tataaaccaa aagccattga aagtgagggg acggttgatt ccattacaac
    11641 caatgaaatc aatgagtttt taacgacgtt cttcaagctc tatcctacag cgacagccag
    11701 tgaactttcc tactatgtga atgacgggat attaaaacca atcggaaaag agtacatctt
    11761 tcaagaactg gtaaatccta ttcacaatcg taaggataat caagtcacgg tatcgctgac
    11821 agtggagtat atcgaccagc agaccaaagc aacgcaggta tctcaatttg atttggtact
    11881 tgaaaagaac gggagtaatt ggaagattgt aaaataacaa atattggtac atgattacag
    11941 atactttgta atcatgtact ctttttgata aaaaattgga gattccttta caaatatgct
    12001 cttacgtgct attatttaag tgactattta aaaggagtta ataaatatgc ggcaaggtat
    12061 tcttaaataa actgtcaatt tgatagc
//
LOCUS       DACCPY010000088        11930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCPY010000088 DACCPY010000000
VERSION     DACCPY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.090"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            50..259
                     /locus_tag="IVO69_002200"
     CDS             50..259
                     /locus_tag="IVO69_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400959.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            466..666
                     /locus_tag="IVO69_002201"
                     /pseudo
     CDS             466..666
                     /locus_tag="IVO69_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1153..1332
                     /locus_tag="IVO69_002202"
                     /pseudo
     CDS             1153..1332
                     /locus_tag="IVO69_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1378..1983)
                     /locus_tag="IVO69_002203"
     CDS             complement(1378..1983)
                     /locus_tag="IVO69_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7400960.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2165..2500
                     /locus_tag="IVO69_002204"
                     /pseudo
     CDS             2165..2500
                     /locus_tag="IVO69_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2665..2892
                     /locus_tag="IVO69_002205"
                     /pseudo
     CDS             2665..2892
                     /locus_tag="IVO69_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3018..3989
                     /locus_tag="IVO69_002206"
     CDS             3018..3989
                     /locus_tag="IVO69_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7400961.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4129..5442
                     /locus_tag="IVO69_002207"
     CDS             4129..5442
                     /locus_tag="IVO69_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7400962.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5485..6351
                     /locus_tag="IVO69_002208"
     CDS             5485..6351
                     /locus_tag="IVO69_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7400963.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6348..7196
                     /locus_tag="IVO69_002209"
     CDS             6348..7196
                     /locus_tag="IVO69_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7400964.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7210..8682
                     /locus_tag="IVO69_002210"
     CDS             7210..8682
                     /locus_tag="IVO69_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7400965.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8699..9571
                     /locus_tag="IVO69_002211"
     CDS             8699..9571
                     /locus_tag="IVO69_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7400966.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9807..11189)
                     /locus_tag="IVO69_002212"
                     /pseudo
     CDS             complement(9807..11189)
                     /locus_tag="IVO69_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11651..>11930
                     /locus_tag="IVO69_002213"
     CDS             11651..>11930
                     /locus_tag="IVO69_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400967.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2037 c         2401 g         3792 t
ORIGIN      
        1 tttaaaaaga aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta
       61 tatttcattt ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga
      121 ctggggtatt gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt
      181 ttataatgct tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga
      241 gaaaaaaaca agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta
      301 gaatgaaaca cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct
      361 aagttttttc atatttgttt gttatgctat acatagatct gcataattag acttgcgctg
      421 cccgttattg aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa
      481 atatttgaga aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg
      541 atcacctcct accccactgt tgactagaca tataaggaaa agaaatactt actttggctg
      601 tgccagttag atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag
      661 aattgatcag aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg
      721 gctcagggca actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac
      781 cactttagaa tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa
      841 tttaaacaag ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc
      901 aaggcaatag atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg
      961 aataaccttc tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt
     1021 attttattgg ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga
     1081 acgattagtt taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca
     1141 gacaggaagg gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat
     1201 ctttttccgg aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt
     1261 cataaagaac taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta
     1321 catggaacat agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta
     1381 caaatcattc gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc
     1441 aatttctgca actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg
     1501 tttttgcgga tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc
     1561 ttttttggcc aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg
     1621 tgcaatataa cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt
     1681 tccagtatta aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt
     1741 cataaaggta actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc
     1801 aacaatgaca caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg
     1861 gatatcttta ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat
     1921 tgccaattgt cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc
     1981 cataaaaact ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac
     2041 tgttatcata gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt
     2101 ttgaatgaat gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag
     2161 ctcggaacag ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg
     2221 tagaaaaggc aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt
     2281 aggattagta actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga
     2341 acaaattaaa acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt
     2401 aattagtgat cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc
     2461 gaatggaata ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc
     2521 ctaccgtgca ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa
     2581 cacaagaata ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt
     2641 tcgtttctat gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc
     2701 attgacgact tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt
     2761 ggtcctgttt aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat
     2821 gaaataaaat tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct
     2881 gaatacgaaa atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata
     2941 ttcctgctaa tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga
     3001 gaggagttgt tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg
     3061 gtgtgggtac ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa
     3121 aagtaatggc tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga
     3181 aaacgaatag tagcaataca atagctctta ttttacctac gatttggcat cccttctttt
     3241 ccgagtttgc ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt
     3301 gtaatgcgaa cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta
     3361 aagtagacgg aattataggt attacctatt cagacattga taaatatgta tcatcaaatt
     3421 taccatttgt cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata
     3481 attatttagg tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt
     3541 gctatgttgg gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt
     3601 acaatatttg tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta
     3661 gcaatttaga ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt
     3721 ttaccatcaa tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg
     3781 aagcacctaa ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag
     3841 actatttact atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa
     3901 cactcatggc gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct
     3961 ttcacgctgg agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata
     4021 ggttgacaga aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat
     4081 taattgaaaa ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga
     4141 atgttagcaa ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt
     4201 aaggagaaaa gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct
     4261 gatgaaacgc ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac
     4321 gagggtgaat ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat
     4381 gaggataaag taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat
     4441 ggaccgaata ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg
     4501 aaagatgcgg acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc
     4561 aaaatgtatg cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg
     4621 ttcaaagatg ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg
     4681 acggaatttc aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat
     4741 atgcaaatgg cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg
     4801 aatggcggcg atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa
     4861 aaatccgttg aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc
     4921 agtcctattg aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg
     4981 acaatggcag atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc
     5041 gtttcagatg caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact
     5101 gagaaaacag agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa
     5161 tctagtcgta ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat
     5221 ttaattaaag atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct
     5281 ggacatgccc gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca
     5341 gttcaagata ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc
     5401 gctgctgaaa tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc
     5461 aatcaagtgt taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg
     5521 ccagctttgc tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat
     5581 gcctttactg attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat
     5641 tttaagcgac tgttcgctga tccaattttt gtaaaaagta tttggaacac ggctaaattt
     5701 gttgtttgga ttattccggt ccaaataggg gcagctttag gaatggcact tattatcaac
     5761 aaaaatcgta aaggaaatat cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc
     5821 ttagtcgtta tttcgatttt atggttgtac ttgttaaatc ctaatgaagg tctggttaat
     5881 acgctatttg caaagattgg aattgcttcg cagccttttt tgactagtcc caaacaagca
     5941 atgtatacgc ttgttttcgt atcggcatgg caaggtgcag gatatcagat gctgctgttt
     6001 ttagggggaa tgcaaaatat tccgcaagac gtttatgagg cagccgaaat tgatggtttt
     6061 tctaagtttc agcaatttcg ctatatcacc atgccattat taaagcccac ggcgattttt
     6121 attttactta ccacattgat ttcggccttt aaactaatca ttcagccaat ggtaatgact
     6181 caaggtggac caatgaattc tactatgaca atggtttatt acatttacca aactggattt
     6241 acagaccgta tggtaggcta ctccagctcc attgcgttaa tctttaccac tatgattggt
     6301 ttaattacaa ttgctcaaag aaaattggta aaggaggatg atccagcatg accaagaaat
     6361 ttggaccgct aatgatcgta gaatattgtt tgctgttcct tttagcggca ttattcattt
     6421 tcccgatgct gtggatggta gtttcctcta tgaagcctga agcagatgtc tatcaaaatt
     6481 tagcttcctt caaagctttt ttacctagtt tgaatccagc taattggttt aaaacttatc
     6541 aagaggttat ttcccggttc agtgtgggaa cttatttatt aaacagtatt ttttatggtt
     6601 taacctttgc ctgtgggtca atcattgtaa actctttggc gggttttgcc tttgcgaagg
     6661 ttaatttttc aggcaagaaa attatctttg gagctttgtt agctctgctg attgtcccga
     6721 tggagacggt tttaattccg caatttacca tcgttaattc actaggctta gtgaatactc
     6781 gcttggcagt gattttacca gcgatggcca gcgcgtttaa tatctatctg tttagaaatt
     6841 tctttatcgc gattccagaa gaaatcattg aatcagcgaa attagatggt gcaaatattg
     6901 ttaaaatttt ctttcacatc atgctgccaa tgtctaaacc agccatagcc acagtaggtg
     6961 tcttatcctt tatctccagc tggaatgatt atatttggcc gttaatggtc ttaactgaca
     7021 gcagcaaatt ttcaatgcaa gtggcaatta ctacgatcaa tactacacag ccagtttata
     7081 tcaatcaagt gatggctgtt ttaaccattg caacgattcc actgattatt gtttatatcg
     7141 ttgcacaaaa atatattctg cagggacttg gcggttctgg aacgggtata aaataggagt
     7201 gaatagtgaa tgttgcaaaa acttttaaag gccaatgagt ttattgagaa aaatgctcaa
     7261 acagtcaatc aagaataccg gaataaattt catttgatgg ctccgattgg ctggatgaat
     7321 gatccaaatg gttttgttta ttatcaagag gagtatcact tgttttatca atattatcct
     7381 tacgacagca tctggggacc aatgcattgg ggccatgcta aaagcaagga tctaattcac
     7441 tgggagcatt tgccagtagc actggcacca ggtgaaagct atgacaagga aggctgtttt
     7501 tccggcagtg ccatagaaaa ggatggcaag ctgtatttga tttatacagg acacaatgtt
     7561 gtggaagggc aagttcgaca agttcagtgt ttggcagttt cagaagatgg cattcacttt
     7621 gaaaaatatg ccggaaatcc aatcatcgca gaagatcact tagcttctgt tgcaacgacc
     7681 gaagatttcc gcgatcccaa agtgtttcag cgaaaagaag actattttac tgtagtcgct
     7741 actaaaactg cagatgctcg tggacaaatt gtgatgttcc attccaaaga tttactaact
     7801 tgggagtttt attccgtatt gctggaggga actccagaac aaggcattgt atgggaatgc
     7861 ccagacttgt ttgaactaga tggcaaagaa gtcttgattc tttctcctat tcaaatgact
     7921 ccacaaggca atgcttacca aaatacgaac tcaactgttg ctttcatcgg tactgtcgat
     7981 tggcaaacag gcaaatttct agtagagaat tttcatgaaa tcgatggcgg tttagatttt
     8041 tatgcacccc agacctgtga aaacgaagca ggccaaagaa taatgattgc ttggatgcaa
     8101 atgtggcagc gtaaaatacc tacgaatgat ctgggccatg gttggtcagg ttccatgacc
     8161 ttgcctagag agctgagggt ggctgataat caattgttgc aacggccagt ttcatcaatt
     8221 tatcaagtaa ttgatccgca ggaaaggatt gaatgggcga agactgaaga gaatcgcttc
     8281 ttactgaagg acttagtata tgaccagcac tatttacgct tggaaatcaa tttaaaacag
     8341 gcaacgaaac tagaacttca ctatgcgaag ggacccgacc atacattact gcttaactat
     8401 gatgttgaac gggagtgctt tactgcttcc agggaacaca tgccgtatga tttatcagga
     8461 gctgaaaagg aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa taattgtcta
     8521 accatcgaaa ttttccgtga tacttcagct attgagcttt ttctaaatga tcgtctgact
     8581 atgagtacaa ctttttatga gacagaaaaa ggacaggatt ttcttttgct ggcagacagt
     8641 gaagtaacct tttcattgga aagcggctta attaatctat agaagggaga gctcagtgat
     8701 gatttatggt tcaatcgaag caggcggcac aaaatttgtg tgtgccatta gcgatgaaga
     8761 aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa caataccttt
     8821 agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga ttggctcatt
     8881 tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct cgaccccgaa
     8941 agtggcatgg caaaattttg atttcatcgg taccatgaaa aaagaatttc cgatcccgat
     9001 ttcttggaca acagatgtca atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa
     9061 aggattgagc agtgtcgttt actatacgat cggtacgggt gttggcggcg gtgccttaca
     9121 agaagggcgc tttatcgaag ggtttagtca tccagaaatg gggcatatgc tcgtcgtccc
     9181 tcatccaaaa gatgactttg ctggcagctg tccatttcat ggcaactgtt tagaagggat
     9241 ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa ggacaagagg ttgcagaaga
     9301 tgatccttat tgggaaatcg aagcctccta tattgctcaa tgtgcctaca atacaacctt
     9361 gatgttttct ccagatgtga tcatttttgg cggtggtgtg atgaaacaac gccatatgct
     9421 gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg tatgtcgaaa cacctgagtt
     9481 aagcaaatat atcgtgaccc cagcattaga agacaacgct ggaacgctcg gttgtttggc
     9541 attggctcga gaagcggtct tacattcata aacgaaaaaa ggctcttcga catttagtgt
     9601 tggtagagaa agatacaact gaaaatttag tcataaccag ttaggcagag tgcttaactg
     9661 gtttttgttt tacttggaag atcaagcctt gaatgagata agttcttgct cgaaagttat
     9721 ggaaatttcg atagccgtag gctacttgta ttgtgcaata gttttttaca aaaaaaaggg
     9781 ggtgcggata ttttttttgg acctttttaa caaatatatc tttgcttctt ccgatattga
     9841 agagggctta gccttcctaa agataactta attcgttgat tattatacca atgaatataa
     9901 tcatccaatt tgtttataaa ctgattgatt gttgtattct tccagtcacg ttgataaaac
     9961 atctcgtttt ttaatcgtcc aaagaaacct tcgcacgctg aattatctgg tgaataactc
    10021 tttttagaca tagagtgaag caagttggct ttgttccaat agtccaacta acaatagcac
    10081 catcgtaaca atcgacaagt ggtgataagt agattttccc tgcgagaatt ctaaattcag
    10141 taatatctgt taaccatttt tcatttggct tctttgcttt aaaattccga tttaataaat
    10201 tggggtgtgc aggagaaatt tccccagcat acgaagaata cttttttcgt ctagaaaatt
    10261 taacaaatag attttcctca gtcatcaatc tacgtacaac tttttcagaa agctttttac
    10321 ccatattttt taaagcttgg tgaattctac ggtaaccata cgcacagaag ttatgattga
    10381 aaatcgtgat aattaatttg acgttctttg taatacttat ctggtttctc aagtgcttgt
    10441 ttttgataaa aatagctgct tttaggaaac tggagctgtt gaagaagctc tttcaaggtg
    10501 aactgatttc gtaaggcatc aatcaatatc gttttttctt gatttgatag acaaagaaga
    10561 ttgacgcctt tttctttttt tagcagttca gccgctcctt cgagtaatgc tttttcgagc
    10621 tgtaattggt gtacttgttt ttgtaagtct tctacttcct ttaaaagtag ctctttttgt
    10681 ttttcctgat tttttaaagt catacacaat ggaaaatcct ttccaagaac cgtgtttttc
    10741 cattgatata gagtaactct actaattcct ccagattcgg ctaaattttt cctattagtt
    10801 tgcggtttat agaatttttt caacacagct tctttttgat cttgtgttaa ctttaccgca
    10861 ctttttcgga gttttctagc tcctggtgca agttcttcac accgctgccg caaggactct
    10921 ctattaggat aaccaagcat gcgaatggtt cgtgcataac attgaccata ttcaaaataa
    10981 tgattcaccg ctattcgttt ttgttcttca gaatactttg atttttttcg gtgctcctga
    11041 tggagctctc cggattccaa atactcttta taccatttcc gtagtgcacc aatcgacgga
    11101 taccctaatt ctcgaatggt tgcagcataa gagcaatcgt attttaaaaa taactgaatt
    11161 gcccttacac gctctttgtg ggaaaacaca cggacattcc tcctaagttt attttttcgt
    11221 ccaggattat gtccgcaccc ccttgctttc tgatgactta ttctggttct ttgagtgtca
    11281 atgcatagat gtgatgtagc tagttagaaa aaaattttga atactctttt tttctttcag
    11341 actccttttt aagtaagatt ttgaagtgtg agttacgggg tcagaaactt aaaagttttt
    11401 gatcctttat tttcaagtct gatttggcag cttttcggca gctcatttat ttatggtcag
    11461 aaatagatgc tgaaatttta atctattagc aaatagtcca gaacgtatga agaggagtcg
    11521 gatatcattt gtcattaaca ccataaatat ttataaaatt cgtgttcacc tacaattaaa
    11581 tttaatatat aatattcgtt gtaagggctt ataatttttt gttaatgaac aagataggaa
    11641 gaaacattta atggacagtt ttcagagatt gtcactagta aatcaatttg agttattggc
    11701 taaatttgag gaccaaaaca gatcttatta tgaaagaaaa atcgaaatat tgagagaagg
    11761 ttacgagtat cattatgatg atgaaatttg gagtgatctt tctgagccat ttccgaaaga
    11821 agattctcga tttgtattag atgtattgaa catgtatcga gatatcaatt ttagtaaatc
    11881 aaagctaaac ttagatgaaa accaaattac taattcatac tatactcatt
//
LOCUS       DACCPY010000089        11835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCPY010000089 DACCPY010000000
VERSION     DACCPY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.092"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            362..805
                     /gene="rplM"
                     /locus_tag="IVO69_002214"
     CDS             362..805
                     /gene="rplM"
                     /locus_tag="IVO69_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7400968.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            821..1213
                     /gene="rpsI"
                     /locus_tag="IVO69_002215"
     CDS             821..1213
                     /gene="rpsI"
                     /locus_tag="IVO69_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7400969.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1302..2468)
                     /locus_tag="IVO69_002216"
     CDS             complement(1302..2468)
                     /locus_tag="IVO69_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7400970.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2523..3173)
                     /locus_tag="IVO69_002217"
     CDS             complement(2523..3173)
                     /locus_tag="IVO69_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7400971.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            3248..3448
                     /locus_tag="IVO69_002218"
     CDS             3248..3448
                     /locus_tag="IVO69_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7400972.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            3460..3786
                     /locus_tag="IVO69_002219"
     CDS             3460..3786
                     /locus_tag="IVO69_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400973.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            3939..4991
                     /locus_tag="IVO69_002220"
     CDS             3939..4991
                     /locus_tag="IVO69_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7400974.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            5067..5285
                     /locus_tag="IVO69_002221"
     CDS             5067..5285
                     /locus_tag="IVO69_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400975.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            5272..7950
                     /locus_tag="IVO69_002222"
     CDS             5272..7950
                     /locus_tag="IVO69_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400976.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            7941..8138
                     /locus_tag="IVO69_002223"
     CDS             7941..8138
                     /locus_tag="IVO69_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400977.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            8142..8753
                     /gene="mobC"
                     /locus_tag="IVO69_002224"
     CDS             8142..8753
                     /gene="mobC"
                     /locus_tag="IVO69_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP7400978.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            8738..10129
                     /locus_tag="IVO69_002225"
     CDS             8738..10129
                     /locus_tag="IVO69_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7400979.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            10345..10659
                     /locus_tag="IVO69_002226"
     CDS             10345..10659
                     /locus_tag="IVO69_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400980.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            10652..11146
                     /locus_tag="IVO69_002227"
     CDS             10652..11146
                     /locus_tag="IVO69_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400981.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            11214..11528
                     /locus_tag="IVO69_002228"
     CDS             11214..11528
                     /locus_tag="IVO69_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7400982.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            11627..>11835
                     /locus_tag="IVO69_002229"
     CDS             11627..>11835
                     /locus_tag="IVO69_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7400983.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
BASE COUNT         4358 a         1688 c         2207 g         3582 t
ORIGIN      
        1 agcctgccgt tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt
       61 atggatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag
      121 gggttgtgac aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac
      181 ccttttatat aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga
      241 aaaacgttga cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca
      301 aagagccccg gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa
      361 cgtgcgtaca acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga
      421 cgcaactgat gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa
      481 aaataaacca actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc
      541 agacaaagtg aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa
      601 ctacccagga ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg
      661 tttgatcgaa acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt
      721 tacaaaattg aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt
      781 tttagacatc acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat
      841 cggcacaggc cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa
      901 aattactgta aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt
      961 aatcaatcaa cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt
     1021 aaacggcgga ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt
     1081 gctagaagtt gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga
     1141 cgcacgtatg gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacagtt
     1201 ttcaaaacgt taatcgttgt tataccaacg atttcagaca ctttctcatt gcgagaaggt
     1261 gtcttttttt cgttctggta atgagaaagg taatgacaat attataaacc agtgtaagca
     1321 atgaattttt ctaacgcatt tcgttgagct ttttcagtaa catgagaata agtgttaagg
     1381 gtagtttcag catttttatg acccaatcga tcttgtgttt ctttgatact tgctccacct
     1441 tctagtagaa gagtagcgtg agtgtgccgg aatccatgaa tagtaacgtg cttaaattta
     1501 tatttcttct ctaaacgttt caatatatta tttgaatagt ccgcatgcaa aggttgatta
     1561 atgtcaccgt ctgcatttac ataggtaaag ataagttgat tttttgtatt tgtatttatg
     1621 ccaaactgca aaagctctcg agcttgttct tttttccaga tttttaacat ttcaatagtt
     1681 tgtgcatcaa ttggtaatat tcgttcggaa gatatatttt ttgtattttt ttgaatcctg
     1741 acagtttttc ttcttgcatt cttctcttca tttcgaatag ttgcatagct ctttctaaca
     1801 tacattagct gttcttcaaa ataaatgtca gaccaatgaa gagcagctag ctctccttta
     1861 cgtataccag tagtaatcaa aagctggaag attaaaatat gttttaaagg ctcttcttta
     1921 ttgagaatat cccaaaaagc ttttagttct tctaatgaaa agtaattttc attttctttc
     1981 ttttcccgct ttggtagatt tgtatttgcc ataggatttc ggtgtaatag ctctgcaaaa
     2041 atggcatact caaatatttg agagctgtaa gacttaagct gtttgatatt cttgaatttt
     2101 aaagcccatt cattgactgc attctgacag tgaacaggag taatcttaga aatggactta
     2161 ttcccgaatt ttggtagtat atgcaagcgg aaaaggtctt ttgtacgttg atatgtcgtt
     2221 tcttgtacac gattcctata agaaggcaac cattcttcat acactgttat aaacaagtca
     2281 ttagaaggct tatgatagcc ggtatcttca atctcgacct tttttcttgc ttcgtgtaac
     2341 ttggcttctt tttcagtttt aaaccctttt tttacatagt attcttgctt tccattttca
     2401 tctttacgaa catacaagcg gacactatat cgtgcaccgc tttttgttgt gtacttttgt
     2461 attgacataa ctaatctctc caatctgata acttggcgag ttggctgatt gaataattag
     2521 ttttattgaa gttttgatat taatcgagct gttatctctc tttttagatt gttaacctgt
     2581 acttgggtta agttgccaaa taactcagat tctaactctt ttctttcatc gatatttaag
     2641 tttaaagctt tattaataaa cttcttttgt ttggcaaata gaatcgtgta ttcaaaggag
     2701 tcctctagaa tactttttaa aaaaagaaag tcggagtcat cttcttgaag tttgacttca
     2761 agtaaagaag tcatggaaaa gtatagatta gataattcag taaaataatc tgagtcaaga
     2821 ctggcaatat tcttgactat tttgtccttt ttttctaaac gagcagtttt tattctatca
     2881 agattaggtt gttctatgga atttgtttcg tctaatccca tcaaataact tacaggaaca
     2941 tggaaataat cagcaaggat attccatgtt tcagtatttc taggatttcg ctcaccacgg
     3001 taatagttcg taagagttgt atagttaata ccagtttctg tactaatttg tttaatggtc
     3061 tttttactgg aatcaatata aaattttatt ctattcaaag taacgccccc tatagtaaat
     3121 tccaaactat tatagcatga aaaccacaaa ttgagtaata ttttaattga tattactcgt
     3181 tttgtggttt aaaatgtgct tattaagata aaaaccacaa agtgaataat ttgaaaggag
     3241 gcgctgaatg ataactctta atgatcaatt cattagaagc ttaagaagac atagagccga
     3301 tcttatactt actaaaaacg atgcagcaaa attgataggt ataaatcgga aaacatatgt
     3361 caaaatcgaa aatgggagca aagaaagtat tagagcatct acttatcaaa agttggtaaa
     3421 ttggttactt aatgatttga aaatctaaat aaatacaact tggcgagttg gctgattgaa
     3481 gaaatagaaa atgagaggag aaaaataatg gatgcaggaa ttacagtaat gttagataaa
     3541 caacaaacga atcaattgaa aaattatgtt tttgaaatga caaaagaggc tattgatcaa
     3601 gcaagaattg ataccggatt agaaagacca tttctaaaag gaaaagagat ggctaaatat
     3661 ttgaatgtat catatacaac ttttttgaaa tttaaaagaa tgggattgcc agttatttta
     3721 ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa aatggatatt gagtcatcaa
     3781 atttaaaaaa cacctcgttt tttaaatttg atgaagtaga aaaaattaaa aagagtgttc
     3841 ccgtcagttt gcagatcggg ggaacactca ataataaagt tctgagaaaa ctttatttga
     3901 atctattata gcaaataaaa tcagaagcag gtgtgacaat gaatgaaaaa gaagttgaaa
     3961 gcttagttga aaagcaaggc tattctaatt ctgatatata tgcagaatta tactatcaat
     4021 atcctatttt tttaaacgta aatgaaaaat ataagaaaat gaaactaagt tctaagttag
     4081 tttatatgtt actaaaagat agaaatagac tttcattgaa aaataacttg tatgatgaga
     4141 atcaaaaaat ttttttgata tttgaaatca aaaatctcgt agaaatttcg ggaatgtcaa
     4201 aaaatacaat cttatcagca ctagatgaac ttcaaaaagt taaacttctt gtgaaaagaa
     4261 aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa tttttattat ttgttaaagc
     4321 ctgttctgtg caaagatgat gtttataaaa ttaataagat aacccatcaa gaaaatagac
     4381 aaaaaaactt gatagttcaa aatttgaact atggaacaaa tcaatattct gttgataatt
     4441 caaaatttga actatgcgac acatcaatga aaacaagaga caaaaacaat tttgctgata
     4501 gttcaaaaaa tgaactagaa ttatataagt cttataaaga tagtaaagat attaaagaat
     4561 cacataatga tgatcttctt gaatcacttt caactaaaaa taataaatta gatgaagaat
     4621 tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat atggggagaa agtattgtga
     4681 attgcttaaa actggctagc aatagtgatt atcgtcaatt cgacaactgg tacaaaaaat
     4741 tcaaatatgc gattagatct gctgaaaaag aacaaaaaat tcaactgaaa attatctatg
     4801 aaatttgtaa taacaagtat tttgtagatc atgttagaga acagctatca atgactttac
     4861 gtgacttgat acgaagagca aaaacagatc atcgtatcaa acaaaaagac gattatatct
     4921 ttgcttctct aaaaaataaa gcgttagaac taattgaatt gcaaatcagc gaaggaatag
     4981 ataagcaata agaatagaat tgagtcaaat gaaatggagg ggtatctcca cttcatttga
     5041 gaactttgaa tataggaggg gttattttga ttaaaaatcg tttaatagat caagtttggt
     5101 gtctagatca aaatacagat agtctcgaat taatcgagac tatttgcttc aacactatag
     5161 ttctcgattt aagagctgaa aatgataatt gtgtaactca tgtcataatt aatcaaaaaa
     5221 tggctcagaa gctctctgag agcctcagaa aatgggctaa gggaggtaac gatgaaaata
     5281 attgaaaaaa agattgccaa taaaaaaata aattggttgg ttgttaattt tcttgttctg
     5341 atagctatga ttatttttac aagtaatatt gctcatgcga gtattttcaa ttggtttgat
     5401 gacacaaaaa gtcaaacaga gtttttgaat aattctcttt atagacctta cctaaaaaag
     5461 tacaatggag atttccttag tcagttcggg atttggctcg gatgggctgt ggtaaaaggc
     5521 atgtttactg tgacagattc gattcaaaat atgattccag atgtgctgga tttgttcaat
     5581 ttcattgaaa gtacagggct aaataatgtt tatcaatcag ccgtaaatac aattgtcatt
     5641 ggattaatga tcctttcatt gatgtttgtt ggttacaaaa tgattactgg aaaaggaaca
     5701 gtggatttaa aatcggttgg aatgaacgta gtgatgtcta tagcattaat acttttaatg
     5761 ccaaccatga tttctagtgg tattcaattt gctaaaacat tttataagga ctctactacg
     5821 attacaaatt cagatgacgg agtcgcttgg tcattgataa aacaaggagt aacagattta
     5881 gcatatatta ataaaacaga tcagtactca agcattgata aaacggagga tcgaaataat
     5941 ttaacacgaa aaaattttca acagacggat ctcacacaag tgttaacgga taaagtgatt
     6001 gataaattgg aaaaagaaaa tccaattgcg gacaatcttc gttatgaatt agttgaaaat
     6061 tctaataatg aatttgttgc tacaaagttt tctgacaact tcttatcaac attttctgat
     6121 agcttgaaat caggctatta ccgataccaa gcaaatctat gggggatttc aattgggttg
     6181 acagcgttag caatcgccta tgtgttcagc gcgtttgtga tcattacagc aatactagaa
     6241 cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa cagatattga gacaggacag
     6301 cgttcaaaag ttgttctatc agatattcta caatgttact taactgttgg gtttcaagga
     6361 tttgggttat caatgtttgc tatgtttatc aattttctga actcaggaca aggtatctca
     6421 acaaatattt ttataaaaac aattgcttat atctgtgccg tttttgtttt aatcaaaggc
     6481 agtggaacag tcatgagata ctttggtgtt gatattggac tcaaagaagg ctatgggcaa
     6541 cttgcttctg cttttggaat gggcgcaatg ctgtttagaa aaggatcaaa tggatttaat
     6601 cgtgctaaag gaagcggaga aggagaagat cgaaaaccag aaaagaattt cggcgaaact
     6661 ctatcaaaaa aagctggcaa aactggaaga gctttgggat atgcccatga acgtggatta
     6721 tccggtttag cttcagatgg agctacaatg gctagtgaaa gagcaacaaa accatttaag
     6781 agtatgcgtg atatagctaa tgataccaag aataagttta aagaaggttt agatgatggt
     6841 acagtgtccg ctattaataa aaatagcaaa cctatgttag ctaaaaataa agaagatgaa
     6901 actggaaaat atgctgattc cgtgccaaca cgtctttcag atagaaaaga tggtgctaaa
     6961 gttgaaaatg ccgatagaat tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat
     7021 aatgctgaat cgaataataa tcctgcaagc gcaattcagc aaaaagtcca acaagatatt
     7081 gaagaacgta aaaatgctat gcacggtcaa gctaagtctg ctgaagaatt aattaatcag
     7141 aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc gcgaggaaat aatgttaagg
     7201 aaacgtgtag aagggcggac aggcgctaat attgacggaa aagaagcgtt gaccaaagaa
     7261 agaattcaag aagctaaaaa ttcaaataca ggattagaaa aattagtcaa agaaaatcca
     7321 caaaattcta caagtagtca tggtggccga tcagtagatg tccgagaaaa tattcaaggt
     7381 tcactgaatg gtcatgttgg acggaatgtt gatgtaagag agaatttaca aagttcttca
     7441 aatgctcaag gtactaagac aattgatgtt cgagaaaatc tgcaaagtag cagtacaagt
     7501 caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt
     7561 aaatcagtgg atgtccgaga gaatgtgcaa ggtagcagtg caagtcaacc aaaaacagta
     7621 gatgtccgag aaaatgtaca aagttctaca agtagtcaag gattcaaaac agtagatgtt
     7681 cgggagaaca ttcaaaaaga tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc
     7741 gttgaagaca gaaagtcagc gaaaaaattt gattcaaatc atgaaacagt gattattgat
     7801 agtgaaatcc gtgaaaatga taagggatca aaatcaagac gaagattcac ttatgaagac
     7861 aatgagttat tcagagatac acttaatgat cctaatccac ttttcgataa tttattaaga
     7921 aataaaaggg ggggattgtc atgcaattaa ttctaaagat tagtacgcat gatttcttag
     7981 aacttgacga attatgtgaa tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta
     8041 attatattga actgatgatt caagaagaat taaagatgaa aaatgaaata gagagcaatt
     8101 tcaaatgttg ctctctagaa aaaaatgtga ggggataatc aatggatgat aaacaaaaaa
     8161 tgtacgaaaa aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt
     8221 atcttgaact gacattgatt gcaaaacaaa ggggaatttc tagagcagac gtttgccgtg
     8281 agcggttaaa gggactcaaa tatgaaccgc cgttgttagc tgaagatgat agggtagttt
     8341 taaccaaaca gttaaatcaa attggtaaca atttgaatca agcggtaaag gcattaaata
     8401 tcattgctaa atattttaga gaagaatctg aagataaaaa agaaaagagc ctacatttta
     8461 gttttgatgc tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa
     8521 agttgatgat tagttatcca gatgacattt ttttggagtc caatcggagt gccccctctt
     8581 tttttaaatt atcagaagta gaaaaagaat atttcgaata ttggatagaa aagcaaacta
     8641 gaccgggtac gaaagaagag tattcccaaa aagttgatcg gaatttaatt cactcaattc
     8701 agattattgc aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa
     8761 aggacgcaag aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga
     8821 aaaatgtgtt gcagtctcag gaataaattg tagtgatgat agtgaggaag cactgcaaga
     8881 aatgaattta gtacgtacca agatgaataa atctaaagat atagcacaag cgtttcatgt
     8941 tattcattcg tttgataaga gtacttctaa agagttaagt attgataaaa tgcacgaaat
     9001 agctgtcgaa tttgcagaga aagcttttcc aaattcgcaa atcattgttg caagtcacaa
     9061 tgataaagat cattttcata gtcatcttgt cattaataat attaacatgg aaacatgtaa
     9121 aaggttgcgt attgacccta aagatttaga actaatgcga aaaattaacg atcaagtcct
     9181 caatgaacat ggtcttgaat cagtgaataa tgagtactat gaaaacttag aaaagagaac
     9241 caatctttat tcaaacgatc cttctaagat gaaatatgga aaaattaatt atcaagagtt
     9301 tgtgcaaaat gcgatcgaag caacgttgga aaaatgcgaa gtgaattctt ttgaagattt
     9361 ttcggatcat ttggcaaaag aatataacat tgaagtttat caattttcaa aaacaaataa
     9421 caaacttggc tatgttcttt acaaacaagg tgtaaatttc tatgagcgta gaggtgagtt
     9481 aattacttta ggaagagata aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac
     9541 ttttagtgca agaaagttag ggaaaagata ttcatttgaa tctgtttctg aacaacttga
     9601 aaagaattta gaagaatcac atataaaaaa tttttttaat atggaaagag agcaagttat
     9661 gtcgctaatt agtggagtaa attacaaaac tggtgagtta aatgtaccgt ttgtggaatt
     9721 agattccatc gcacaaaagc aatttagcga ccgtgaaggc gatgaaaaat tcattaaata
     9781 tgatgacttc ttgcgagagg tagacaagca ttttgtaaat acaatgtctt tagaagatga
     9841 tcaagaatac acgattaaac ttcatacgga tgtgaatcct caaactttac gtagtgcgaa
     9901 aagtaaaaca atttctatta aaaagactga tcagaaagag ttatttctac cggcaaaggg
     9961 ttttgaaaat tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat
    10021 aatgaatgac gatgtaaaaa tgtatcgtgc taaaaaggat tatgaattca cacaacaaat
    10081 gattgaaaat cagcaaaaaa ggcaacaaaa ttttgaaatg gaattctgaa tgggcttgtt
    10141 agtggacttt ccagtttttt gcctatgaag agttgcttgg aaagaagcag tttttaggac
    10201 atacgccagc tacccatcta cttgccagtc aatccgcccc gctcacgcta ggctactttc
    10261 tggcaagtca gatggcgggt aaatctctga gggagcaagc tccctacaga ctccctccca
    10321 aaaaaagaaa ggtggaagaa aaaaatgaac tatccatatt ttaaagtctc ggcttcagaa
    10381 gaaactaaag aaatattcaa caatttttac aaccaaaaca aaggaatttt tggttcaaag
    10441 gcaaatatgt ttcgtgttat ggtttcaaat ttaccagtgt tagctagtcc atcaaataat
    10501 aaatttaatg atcctgaaag cattaaattt gaacaaaaaa tttcagaact agaatcaatg
    10561 ataagtaatg aagtaattga aaagctggat gacattgatc aaaagttatc gtattctcta
    10621 aaaaacaaat acaaaacgga ggagaaaaaa gatgtttaaa gtttacttcc cgtttcagtg
    10681 gctcgttaaa ggaattcttt tagtgctact aggattgatt ctttttggaa gttatctaac
    10741 aattaaagcc agacattttc cagaagaaaa gccaataaaa attgtaacaa aagatttaaa
    10801 aataggtgaa acttatcagg cagatggggt tgaactaaca tttaaaaata ttgaagtgac
    10861 cgaaaaaaga aatgaatttg cacaacatga tcgtgtatta caagtgacct atacacttat
    10921 aaatgataca gaaaatccga tacgtccaca acaatggtcg attgaaaata aggatggtga
    10981 agaactttat gagtatgata tgatattgga taatccagag tatattgaaa gtggcaaaga
    11041 agaaacagta aattactatt atgaattacc gattgaaagg caggactacc aaatcgtagt
    11101 acgtagagag aatggttaca tagttgggtt gccatttact ttataagttt tattttgtta
    11161 gtagttttta aggtaatatc agttttgtga tttttgaaaa tggaactggg agaatggctg
    11221 aggaaaataa agttgttccg tttgaaacat tagtgataaa taaggaagag tttgagcaac
    11281 tttttcttga tttttttatt caatatgaca tgatttatga ttttagttcg tcactagatt
    11341 tatttgaatt agaagcagaa ataatagaaa acagtcgaga gaagaatggt aaaatcatta
    11401 tttcagtaga aaaattcaat gaattattct cacaaacatt tgaaatagat ccagactatt
    11461 atcaaccaca agaagttttt cgagagttta gaaataagat tattggtttt agtcaaacta
    11521 caaaatgaaa gcttaataaa gcaagtgttg atagaattat tcctcaacgc ttgctttatt
    11581 ttatgggcac ttcataatag aggtatagag aagagaggta ttcattttgg atactattac
    11641 tccaacaaac ttcaaagaaa atataggtaa agtgttgcaa gaagtagttc attcaaattc
    11701 tgaaatagaa attactaggg attctaaaaa tggattgaac gatggcgtca ttatgatttc
    11761 taagagaaaa tgggagttgt tacaagaaga actttattta caacgaacgg gtacatgggc
    11821 ttatgtatct gattt
//
LOCUS       DACCPY010000090        11510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCPY010000090 DACCPY010000000
VERSION     DACCPY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.093"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IVO69_002230"
     CDS             complement(79..2154)
                     /locus_tag="IVO69_002230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7400984.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVO69_002231"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVO69_002231"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7400985.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVO69_002232"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVO69_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7400986.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IVO69_002233"
     CDS             complement(4412..5311)
                     /locus_tag="IVO69_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7400987.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IVO69_002234"
     CDS             complement(5326..5727)
                     /locus_tag="IVO69_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7400988.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVO69_002235"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVO69_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7400989.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IVO69_002236"
     CDS             complement(6198..8390)
                     /locus_tag="IVO69_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7400990.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IVO69_002237"
     CDS             complement(8414..9385)
                     /locus_tag="IVO69_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7400991.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IVO69_002238"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IVO69_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7400992.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3107 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
//
LOCUS       DACCPY010000091        11412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCPY010000091 DACCPY010000000
VERSION     DACCPY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.094"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..864
                     /locus_tag="IVO69_002239"
     CDS             <1..864
                     /locus_tag="IVO69_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7400993.1"
                     /translation="PMSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMIT
                     SGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYE
                     GQILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEF
                     VQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTE
                     TEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLY
                     ADMYKLQSSGI"
     gene            1190..2041
                     /locus_tag="IVO69_002240"
     CDS             1190..2041
                     /locus_tag="IVO69_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7400994.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2138..4018
                     /locus_tag="IVO69_002241"
     CDS             2138..4018
                     /locus_tag="IVO69_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7400995.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4058..4960)
                     /locus_tag="IVO69_002242"
     CDS             complement(4058..4960)
                     /locus_tag="IVO69_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7400996.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5191..6594
                     /locus_tag="IVO69_002243"
     CDS             5191..6594
                     /locus_tag="IVO69_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7400997.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6594..8027
                     /gene="ascB"
                     /locus_tag="IVO69_002244"
     CDS             6594..8027
                     /gene="ascB"
                     /locus_tag="IVO69_002244"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7400998.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8172..9098
                     /gene="trxB"
                     /locus_tag="IVO69_002245"
     CDS             8172..9098
                     /gene="trxB"
                     /locus_tag="IVO69_002245"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7400999.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9312..11039
                     /locus_tag="IVO69_002246"
     CDS             9312..11039
                     /locus_tag="IVO69_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7401000.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11111..11404
                     /locus_tag="IVO69_002247"
     CDS             11111..11404
                     /locus_tag="IVO69_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7401001.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
BASE COUNT         3702 a         1970 c         2607 g         3133 t
ORIGIN      
        1 ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt tgctggtagt
       61 cgtatcttga agattatgga tacagaagaa tatgcaccac aacaaaaccc tggagcagat
      121 caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta tgatggagag
      181 cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt tgcattagtg
      241 ggacacaccg gaagtgggaa aagctcgatc atcaatgtgt tgatgcggtt ttacgaattt
      301 tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat ggaagagtta
      361 cgtaagaaaa tggggatggt cttgcaagat gctttcttgt tttatgggga tatcgcagga
      421 aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc ggctgagttt
      481 gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa
      541 catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg aacaatcgtg
      601 actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac tgaaacagaa
      661 ggattgatcc aagaaggatt ggcgaaaatg cgacaaggac ggacaacgat tgcgattgcc
      721 catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa aggtcatata
      781 gtagaacacg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc agatatgtac
      841 aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agcattttga cctaagcact
      901 agagtgaaaa taaaacatag cttcccatat ttttcatctg tgagagttag tgtcgtagtt
      961 accagattta aaaaagacga cgtcagctag tcagaagtgc ctaatagccc ctaagaaagc
     1021 gttgacaaaa tcgtttgctt tcgataatac aaagaaaacg atacaacttg gatagtgatt
     1081 gcctaatgca agctaaacct gatgagtctc ccatggtttc atggacggct ttttgggttt
     1141 ttctttttgt tttggataca gccctatata tcatggaaag gagcaaaaca tgctgattga
     1201 gaaagtactg aataacaacg tcgtaataac cagagatgaa agaaatcagg aaatgatcgt
     1261 gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg actcatgcat
     1321 cgacaaaaaa tttcagttga caaatgacgg actttcacaa cagttccaag aattggcaca
     1381 agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata caaaagagga
     1441 tctacaagtc catttgaatg attctgtgta tattgcattg acggatcatt tgtatacagc
     1501 tatcgaacgt caaaaaatgg ggatctttgt gccaaatgta ttgctgtatg atatcaaaca
     1561 actgtttcct aaagagtatg ccattgggaa aaaaacagtg aagaaaatct cggataaata
     1621 ccacatcaag ttatcagaag acgaagccgg atttatcgca ttgcatttag tcaatgcaca
     1681 aggcattcaa agccagcaag acaataccta cctgtttacc acaacaatac aggatatttt
     1741 aaatatcgtc cgctattact ataagattac atttgatgaa gattcaatcc atttttttcg
     1801 attcgtcaca cacctacgtt tttttctgac acgcgtccta gatggagaga aatcagaagt
     1861 gggagaagtt tctgaggaat tgttccaggt tattcaaaaa aaatataagg acacgtttag
     1921 ttgcattgag aaaatagcag actttttaac cggtgctttc gactaccaga tgtccacgga
     1981 tgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc gttgtaaata
     2041 aataaaaaag gaggacaaat taacaagaac gtatgaaaat aagggtagtg acattcagtt
     2101 ggctaaacct tccactaatt aattggaggg aatagaaatg ggaaaatacc aggaattagc
     2161 agaaaacatc gtaaagaata tcggaggaaa agaaaatatc cgtagcttga cacactgtgt
     2221 gacgcgactt cgtttcaaac taaaggagga aaataaagca aaggatgatg ttttgaaaaa
     2281 gatggaaggg atagtcacca tcatgaaaag cggcgggcaa tatcaagttg taatcgggaa
     2341 tcatgtgcca gatgtctatg cagaagtact gacagtagcc gggatttccg atgaagcaga
     2401 agaagaggtt tcttcaagta gtaatctgtt caatcgatta atcgacatat taagtggctg
     2461 tttccagcct tttttaggtg ctttggctgc agcgggaatg gtcaaaggat taaatgcttt
     2521 gttcttgttt ctcaaatttt atactgaagg atcgggtacg tacatcatgc tgaatgggat
     2581 aggggatgca attttttatt tcatgccagt gatcttaggg tacacagcag cgaaaaaatt
     2641 tcatctgaat ccgatggtgg ggctcgtgat tggtgcagca ctttgttatc cgactgtaca
     2701 aggagcagca ctacaaagtg gtttccctga tggcagtcct gcgccttata gcttgctggg
     2761 tcttccggct tatgaaacat ttttgggtat cccatgggtg ggcagcagtt ataccagtag
     2821 cgtcgtacct gttatcttca ttatcgcatt tgccggacaa gtccaaaaaa ttgccaagaa
     2881 atggattcct gcagtcgtcc agaccttttt ggttcctttt tctgtgttat taatcgcact
     2941 tcctgctggc tttttgatta tcggtccgat tgtcagtata ctgacagatc tattaagcac
     3001 gggattccaa gcattgatgg acttttctcc agctgtgtat ggtgcagttc tagggtttct
     3061 ttggcaagta ctggtgattt tcggacttca ttggagtatt atccctcttg cgattatgca
     3121 gctagcgcaa aatgggtgga gtcagatgtt agtaggcgtt tatggcgcga gttttgcaca
     3181 gacagcagtg gtattggcga tgtatttcaa gttcaaggat caaaaaatga aggcgctttg
     3241 catgccagct gtgatttcag gaatcttcgg cgtaacagaa acggctattt atggaatcac
     3301 cttaccaaag aagaagccat ttatttactc gatgattggt ggtgctgttt caggaatcta
     3361 tttgatggca tctggaacaa tcagctacac aatgggcggg ttagggattt ttggggtggt
     3421 aaactacatc acggaaacag acgccagtgg tatgtttcaa gcgtttatcg gcattgctat
     3481 tgcttcggta atcgggtttt cgctgacttt tttcttttgg aaggatgaag caggtgaaac
     3541 agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac aaggacaaat
     3601 cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag gtcgaggaat
     3661 cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag tcatgaccct
     3721 gttcccgacg aaacatgcaa ttggaatggt ttctgaaaca gggctggaat tgctgattca
     3781 tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag tgcagcaagg
     3841 agaaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa tcgaaaagga
     3901 aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt tggatatttt
     3961 ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac tgacctgaac
     4021 ggttaggtga agaatggact aggcttaact attcttttta ttccacttct ttattttcaa
     4081 aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat ttttgaccga
     4141 cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca
     4201 cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt gaatacagtc
     4261 gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt gaatcaccga
     4321 tactggtcaa agctaaaatt ggaacatcat tttgtttcaa tatttctgcg gtacgaagga
     4381 tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt
     4441 acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct
     4501 tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga
     4561 ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag
     4621 aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa
     4681 taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat
     4741 aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt
     4801 ttgctacacg gatgagtgta gacgggtgga catagcatcg ctcagcgatc tcttggatgg
     4861 tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca ttttctgaag
     4921 gagaaaagga gatttctttg attttttcac gtaataacat gtagttacct tcttcttatt
     4981 tttttacgta aattataact tttttgaaaa tgttttgtaa acggatttat actatataac
     5041 ttaaaataga ctttttttga caagatattt acaaaaatcc actagaaaac agtaactttt
     5101 gtaaatgtta ctggaaagaa acggatttct attgtttgtt ttgtttacaa aaaagataat
     5161 gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat cgctacgatt
     5221 ggtggaggca gcagttacac ccctgagttg atggaaggat ttatcaaaag atatgaggaa
     5281 ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc
     5341 gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa aatctatgca
     5401 actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca gtttcgtgtc
     5461 ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg gatgattggg
     5521 caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc agtggtcttg
     5581 gcaatcgttg aagacatgaa gcgtttatgt ccaaatgctt ggctgatcaa tttcgcgaac
     5641 ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa agtgattgga
     5701 ttatgcaatg tgccagtaat ggcgatgatg acagaaccag caatgcttgg caagaaaaaa
     5761 gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa agtagcagat
     5821 aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga agacaatgga
     5881 ttgccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca aatgaatatg
     5941 atcccttgtg gctaccaccg ctattactac cgtgaagaag aaatgctgga acatgcttta
     6001 gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga agcagagcta
     6061 tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca aaaacgaggt
     6121 ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg aaataaagaa
     6181 acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc ggcagattgt
     6241 gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc gtttggttct
     6301 cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt actgacgatc
     6361 gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat caatccgctt
     6421 atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc acaccagcag
     6481 tatttaccgc aattcaagga aacaatcagc cgattaaaag aagaaggaat cgaagttcaa
     6541 gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt ataatgggat
     6601 tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag ggcggttatg
     6661 atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa gaccgttttc
     6721 ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt tatccagcaa
     6781 aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt gcagaaatgg
     6841 ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg ggggatgaaa
     6901 aagagccgaa tgaagctggc ttgaaatttt atgaggatgt ctttaaagaa tgccaaaagc
     6961 acggaatcga gccacttgtg acgattacgc attttgattg tccaatgcat ttgatcaaag
     7021 aatacggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg tgtcgagtca
     7081 tcttcaatcg atataaagga cttgtcaaat actggctgac attcaatgag atcaatatga
     7141 tccttcatgc accattcatg ggagcaggac tgtactttga agaaggagaa aacgaagaac
     7201 aagtcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca acgaagattg
     7261 ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga gcatattatc
     7321 catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga gaaaattatt
     7381 tcttcattga tgttcagtca cgaggagaat acccagctta tgcattgaaa gaaatggaac
     7441 gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa gaacatactg
     7501 ttgattttat ctctttttct tattattctt cccgagtttc gactaccgac aacgaactct
     7561 tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg caagcaagtg
     7621 aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat atttatgatc
     7681 gttatcaaaa accattattt attgtagaaa acggcttagg tgcagtcgat acgccagatg
     7741 agaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat atccaggcga
     7801 tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact tggggatgta
     7861 ttgatttagt atcagctggt accggtgaga tgaagaaaag atacggattc atttatgtcg
     7921 atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca ttcaactggt
     7981 ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca atgatttata
     8041 aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga gaaacgcaag
     8101 cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat taagtaaagg
     8161 agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg atgactgctg
     8221 ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt gcccctggcg
     8281 gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg atcatgggcc
     8341 ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa aatgcttatg
     8401 gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt gaagataaaa
     8461 gttacgaagg aaaaacagtc attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac
     8521 ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt gatggtgcgt
     8581 ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta gaagaagcca
     8641 tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat gaattacgtg
     8701 cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt atgtgggata
     8761 cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa gcacgtaatg
     8821 tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat gttggactgg
     8881 atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc tggattccaa
     8941 cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat gttcgtgaaa
     9001 aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga caacaagtgt
     9061 ataaatactt ggaatcttta gaagaaacaa ctgcataagt ttaaaaagag tagaagaacg
     9121 ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct gatttatgcg
     9181 aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt ttttggtcct
     9241 catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt tcagaaagag
     9301 gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag
     9361 aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg
     9421 ctttttatgc accgttggaa ttcggtactg caggcatgcg gggaatctta ggacctggga
     9481 tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca cgtttcatgg
     9541 atacacaagg tgaagaagca aaacgccgag gtgtcgctat tgcttatgac tcacgccatc
     9601 agtcaccaga atttgcaatg gaagcagcca aaacactggc acatcacaat atcccgtctt
     9661 atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt catttgaaga
     9721 cgtttgcggg aatcatggta actgcatctc acaacccagc ggcttacaat ggatataaag
     9781 tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg acgaaatatg
     9841 tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa gctaaacata
     9901 gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa gaaatcaaag
     9961 cagtgacgat tgatcgtgaa ttggtagaaa caatggggaa agatttgaaa cttgtgtaca
    10021 cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg
    10081 aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact acggtcaaat
    10141 cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga gaaaaagaag
    10201 gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca gcggttcgtt
    10261 tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggggcattg tttgctcaat
    10321 atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg gtattgaaat
    10381 caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct accatgttca
    10441 atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaattcgaa gaagaccatt
    10501 cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa ccttttgttc
    10561 gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct tactataaaa
    10621 aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat ggctattttg
    10681 cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa atctctgctt
    10741 tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa gttattcaaa
    10801 cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa aaactaacaa
    10861 caccgccatc cgacgtattg aaatatacat tggaagaaaa tggctggatt gctatccgcc
    10921 cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagc tctacagaag
    10981 acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca gaagaataaa
    11041 aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga
    11101 ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc aattattcaa
    11161 aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg gggaacaaaa
    11221 tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc atcaactacg
    11281 tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga ttttgtatag
    11341 tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg tggaacatca
    11401 ataacgatag gt
//
LOCUS       DACCPY010000092        11253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCPY010000092 DACCPY010000000
VERSION     DACCPY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.095"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVO69_002248"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVO69_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7401002.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVO69_002249"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVO69_002249"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7401003.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVO69_002250"
     CDS             complement(2169..2906)
                     /locus_tag="IVO69_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7401004.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVO69_002251"
     CDS             complement(2997..3524)
                     /locus_tag="IVO69_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7401005.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVO69_002252"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVO69_002252"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7401006.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVO69_002253"
     CDS             complement(4401..4886)
                     /locus_tag="IVO69_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7401007.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVO69_002254"
     CDS             complement(5039..5236)
                     /locus_tag="IVO69_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401008.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVO69_002255"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVO69_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7401009.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVO69_002256"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVO69_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7401010.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IVO69_002257"
     CDS             9211..9423
                     /locus_tag="IVO69_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7401011.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IVO69_002258"
     CDS             9425..11104
                     /locus_tag="IVO69_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7401012.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3588 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaa
//
LOCUS       DACCPY010000093        11206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCPY010000093 DACCPY010000000
VERSION     DACCPY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.096"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(111..1154)
                     /locus_tag="IVO69_002259"
     CDS             complement(111..1154)
                     /locus_tag="IVO69_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7401013.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(1144..1635)
                     /gene="coaD"
                     /locus_tag="IVO69_002260"
     CDS             complement(1144..1635)
                     /gene="coaD"
                     /locus_tag="IVO69_002260"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7401014.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1632..2192)
                     /gene="rsmD"
                     /locus_tag="IVO69_002261"
     CDS             complement(1632..2192)
                     /gene="rsmD"
                     /locus_tag="IVO69_002261"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7401015.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2311..2649)
                     /locus_tag="IVO69_002262"
     CDS             complement(2311..2649)
                     /locus_tag="IVO69_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7401016.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2633..3070)
                     /locus_tag="IVO69_002263"
     CDS             complement(2633..3070)
                     /locus_tag="IVO69_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7401017.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(3060..4184)
                     /locus_tag="IVO69_002264"
     CDS             complement(3060..4184)
                     /locus_tag="IVO69_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401018.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4205..7633)
                     /locus_tag="IVO69_002265"
     CDS             complement(4205..7633)
                     /locus_tag="IVO69_002265"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7401019.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7659..8822)
                     /locus_tag="IVO69_002266"
     CDS             complement(7659..8822)
                     /locus_tag="IVO69_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7401020.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8825..9127)
                     /locus_tag="IVO69_002267"
     CDS             complement(8825..9127)
                     /locus_tag="IVO69_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7401021.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9241..11013)
                     /gene="recQ"
                     /locus_tag="IVO69_002268"
     CDS             complement(9241..11013)
                     /gene="recQ"
                     /locus_tag="IVO69_002268"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7401022.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3054 a         2447 c         1912 g         3793 t
ORIGIN      
        1 ctaattacgt caattttttc ttttgttttt atttttatcg tgaagatttt tgacgaaaaa
       61 gaggggttgt gacagaactc gtgtcacaac ccctctttaa ttatttagtt tcatttcatt
      121 ttctctagat agtcaagcgc ttcttgtact gtttttactg gtacgatttt cattgaggaa
      181 ttcaattttt tagcggcagc ttttgcttct tcgtagttgg tttttatttc aggatattct
      241 ttcttaacat cgtctgaaat cgtatcatct ggtgcaaaga aaatctctac gccagcttta
      301 tccgcacttg ccactttttt atcgattcct ccgattcggc caacttctcc ttcactgttg
      361 atcgttccag ttcctgcaat ttgcttacca tgtcgcaagt ttttaccagt gacttgttca
      421 tagatttcta aagtgaacat caatcccgct gatggcccac cgatagagcc agaattaata
      481 cgaacatctg tatctgattc gatctcggta tggtcagtaa gcgtgatacc tattcctgcc
      541 tttttatctg ttggtaactc gatcagatca ccagaagctg ttttttcttc accattatgc
      601 aaataactga tttcgattgt ttgtccaact ttttggcttt ttacataatc cataaatgct
      661 tctgtacttt gaaagctttt cccgtctact tttgttactg tatctccaac tgaaagcttg
      721 ccaaagaaat tcgatgtttt ctctacactc atgacatata cgcctttgaa ttccatttta
      781 taaggcttgt ctgcaagttt taacgcttgt tcgattgctg cattttggga agaatccata
      841 taatactgtt gcatttgatt atattcttca tttgtcgccc caccagtcaa ttcttcttca
      901 ctgatcaatt cttcaaaagg ggtcatttta gcttttaaag ctaagaaacc tgtagcttgc
      961 ctgataccaa cagaggtaag agagaaagac ccagaacctt tatcttcctt atcattgacc
     1021 gtaattagtt ctttcagatc gatcgtggct cccggtgatt ctacataata cggcaaggga
     1081 actactacaa atccaatgat caacattcca aatatgataa tcagtactgt tttcaaccat
     1141 tgtttagctt tcatttgatt cacgctttcc agctaaagct tcgttgatca ctggcggtaa
     1201 gtagctgctc acatctccgc caaagtgcag tacttctttt aaaatactgg aactcacgtg
     1261 actatattct ggttttgcta ataagaagac agtttccaat tcatcatata gatgatgatt
     1321 catctgagca atctctcgtt catattcaaa gtctttgata cttcgtatgc ctctgatcaa
     1381 cgcatctgca ccaatctctt ttgctgtttc taccgtcaat tgattttctt gatgcatgac
     1441 ttttacatta ggaagatgag aaacagcttt tgtgatcaat gtcattcttt cttctggggg
     1501 aaacaaactt ttctttgaag tattgataaa aacaccaatt actacctcat cgaataattt
     1561 agccgcacgt tccaccgtat ctaagtgacc ttttgtaaaa ggatcaaaac ttcctgggaa
     1621 taatgcacgt ttcattgatt tccctctttt ctatagatag ttacggccgt tatgccataa
     1681 atctgttttc ttatttgatg gaatccagcc acagtttctg gcaattcgac tgttttatcc
     1741 gtttcgcata cgataactgc ggttttagaa aaaagtccca gttcttccat tttatggatt
     1801 tgatcgatta ttttttgttt tgcatatggc ggatcaagaa acaagtagtc gaattgttgt
     1861 ttctccgctg ctagccgttc gattgctttg tccgcatcaa gttttattgt ggtaaatttc
     1921 tctggttctt tcgtaatttc gatgttttct ttgatgattt ttagcgcttg gtaatttttg
     1981 tctacgcata ccgcatgact gcagcctcta gatactgctt cgatagctaa accaccgctt
     2041 cctgcaaaca gatccaatac tgtctctcca tcaaaatatg gtccaatcat attgaatatg
     2101 gattctttta ctttatctgt agttggtctg gtgttcgccc ctgctaaact ttttaaacgg
     2161 cgcccgccat aatttcctga tattatccgc atctactcac tccttagaaa tggtactggt
     2221 actagtatac caaaaaaata cttggacaat gatcaaaaat cgatcgttat ccaagttatt
     2281 ttttatttaa aaactgctca taagagattt ttaaagatgt tcagctaatc ggatttctgt
     2341 atttagctct tcaatttcga aaccttcgtc ttttaatact gttttgtttc ctattttttc
     2401 ggcaaaattc atttcgatat ccggtcggta agaacgttct acgtttcgaa caaaatgtaa
     2461 tttacggatt cgttcttctg tttcttccaa tgtctcttca tttaagtaaa gcacgacata
     2521 tttcattctt cttgagacat aatgcaacaa gccgaacttt ttcagattct ttaattgttt
     2581 taagctgtag acccatacga tcagcccgcg tcgttttgtt atttcaaatt cactattgtc
     2641 taattgcatg acaattacct ccgcatccaa agtgtttttt cgtttcaaaa aacgggctac
     2701 ctgcatctac tttgatttca ttagaaactt cttcggcaag tacttggaca atttgatcca
     2761 acagactttg cagctgtgtt tcactatatc ggaaagctgc aacatgctca tccatatcaa
     2821 gctgtctttt tgcttttcga acagctcgtt ttttttcacg ataatcaggt gcatactcac
     2881 catatgccgc tacattttca aatgcttctt tcttagacaa gaacgcttct ttcaactggc
     2941 aaacttttgc agattcttcc attgactgtt tatgttcaag ataagaagcc atcgttttac
     3001 tcttttttat catttcagcg acttggaggc attgatcctc cagttgaaac agctcttcgt
     3061 taacgatcac tgctatcact actttctccg ttttcttctt gcaataagac taacatattt
     3121 tctttagaaa aagcaatgcc tacagaatcc gttcccgggt ccatgaaacg gtcatggttg
     3181 taaggatcgc tgtaccaata taaaaacgaa aaagtagcgt caatcgccgg atgaataaat
     3241 agcccactag tattttctaa tttttgttta ctgattaact tctccaattc atcattcgtc
     3301 agtgagaatt tcactgtcgt ttgagcactg cctagcgatt tttgccaatc agccatacgt
     3361 tcctctgaca atatttcctc aggccttgtc aaaaaatcat gtagtagcag ctgactgtgg
     3421 ttttgaaagg caagtgtttg attgtatatg ggaagttcgt cttctcttct aagcatattt
     3481 aataaagtcg tactttttcg ttctttaacg cgattgattg attcccaatc agccgattta
     3541 tctgcttgat aatgtggaag gccttcacca aacacttgat atggcaatag tttcaaaaga
     3601 ctatctttat ctaaatacac aactgaaaat aggccgtttg ttgtttgatc aaaaaataaa
     3661 atagcaaaag tgtcgttatc aaaagcaatt agcggacgat aattcatatc ctcttccatt
     3721 agttcgattc caacttctgt atcatggtac tcaaaagtga aattagtata gatagtagtg
     3781 attttcgtaa gatcttgcat cgtcatccct aaataaaacg gcgcgatatc agcaggccct
     3841 gtttgcagta ctttaacagt tgtgactctg ctgtcttgga tgttcgcttc aaatgtgaga
     3901 tgactagctt tattttcata atatcttgtt tcaaaaccaa atccgctagt taatcgatca
     3961 taggcttggc caaaatcctt ttccagctga tcgatcggtt ttccgatata agtagaaaaa
     4021 ccagttgctt taagctcctg gtatttccac gttgtcattt ggtttgatat ttgttgatca
     4081 gagttttcct tcacttcaag ttttggcgga aacaagactg gttccatgta gaatgccaca
     4141 ataactaata gcagaatcag ccaaaacaaa cccagtcttt tcatgtttcc ccacctttcg
     4201 cttattattt ctcagtcact tcaatcaaaa ggtcaccaga cgaaatagtg tctcctgaag
     4261 acacatatac atgtgcgact tctccgtcaa aacgtgcttc gatggccgtt tccattttca
     4321 tcgcttcagt aatcatcaac gtatcacctt ttttaacgtt atcgcctttt ttcactaata
     4381 catccaaaac agaaccagac atcgttgctc caatttgttc tcggtttgtc ggttcagctt
     4441 tcaatcgggt ttggaccgta ctgatgatcg aattatcttt gatagtgatt tcacgtcttt
     4501 gtccattcaa attgaagaac agtacgcgat tcccttcgat atcgggttct ccgatttcat
     4561 ctaaacgaat gataagcgtt tttccgcgtt cgatttgtac attgattgtt tcacccaaac
     4621 gtataccttg gaaaaatgtt ggtgtgtcca gtaatgtaac atcgccaaat tgattatatg
     4681 ctttttgata gtccaaaaag acttgcggat acatcaaata gcttaatact tcgtcttgtt
     4741 ttggttcata gccaattttt tctgccaatt ctttttttac ttcgttgaag tcgactgatt
     4801 cagccaaaag acctggacgt tcattcaatg caggtctgcc tttaagaata atcttttgca
     4861 attttttagg aaatccgcca acgggttgcc ctagttctcc ttggaagaaa gtcacaacag
     4921 attcagggaa acttagtgtt tcgccttttt cataaatatc ttcttctgta agatcatttt
     4981 ggaccataaa tagtgccata tctccaacta ctttagatga tggagttact ttaacgatat
     5041 cgccaaacat caagttgaca gtatgataca tttgtttgat ttcatcccag cgatgaccta
     5101 atccgacagc ttttgcctgt tgctgtagat tcgaatactg tcctcctggc atttcatgca
     5161 tatatacttc tgtttcagga gcattcaagc cgttttcaaa cggtttgtaa tacatgcgca
     5221 catcttccca ataatggttt aatttctgtg cattttcaat cgtgatttct ggcagccgtg
     5281 gaccattgac cagtgcataa tacaagctgc tcatgctagg ctgactagta ttcccactca
     5341 ttgcactcat tgccacgtca acgatgtcta ctcctgcttt tgaagcagca gaataagtga
     5401 tgattccatt cccgctggta tcatgagtat gcagatggat tggcagatct gtcgtttctt
     5461 tcaattcact aatcaaacga taagctgcct gcggtttaag cagcccagcc atatctttga
     5521 ttgcaatgat atgcgcaccc agttgttcta gttcctttgc catgtcttta taatactgaa
     5581 cattgtattt tgcccgactt ggatcgttga tatcgccagt atagcaaata gctgcctctg
     5641 cgattttacc agtatcccgt acagcttgga tacttttttc catttgtgga gtccagttca
     5701 aactatcaaa gattcgaaat acatcgattc cttgcgctgc tgcttctttg acaaattcca
     5761 ccaatacatt gtcaggataa ttagaatacc caacggcatt tgaacctctg aaaagcattt
     5821 gcagcaacgt attaggcatt aaagaacgaa tttttctcaa ccgttgccaa ggatcttcat
     5881 tcaaaaagcg gtaagccaca tcaaatgttg ctccccccca catctcactt gaaaagagtt
     5941 caggaagacc ttctccagtc aaacgtgcaa tctgcttgaa atcctttgtc cgaacacgag
     6001 ttgctagcag actttgatgg gcgtctctaa atgtcgtatc cgtcaacaac aaattttcct
     6061 gtcctttgat ccattcgacc aaagcatcgg cacccttcgc atctaataca ttcttagccg
     6121 ttacgtaatc agttcttgtg attagatctt taggcatacg tggttcttca taaaatggtt
     6181 tttcgccact ttcgatacct ggaaaaccat tcacagtaat ttcaccgata tatttcatcg
     6241 ttttgtttcc gcggtctcgt agtcgaggaa attcaaataa tgttgctgta ctatcaatga
     6301 aggttgtttt tgcatttcct gattggaatt caggatgtgt gatgacattg agcataaatg
     6361 gaatgtttgt tttcactcca cggattctaa attctttcaa gcaacgttcc attttctgaa
     6421 tggctgtttc aaaggtagca ccatgcgtac atacttttac tagcaatgaa tcaaaatacg
     6481 gtgtcactac atagcccgcg taagcatttc cgacatccag acgtacgcca aaaccaccag
     6541 gtgaacgata cgtatcgatt ttccctgtgt ctggtaaaaa gttattcaac gggtcttctg
     6601 tagtgattcg gcattgaatg gctgaaccat ttaatttaat tccttcttgc tgaggcaatc
     6661 cgatttcttt atgcagatcc aaaccttgag caatcaacag ttgcgttgtt acgatatcaa
     6721 tatctgtaat catttctgtg atcgtatgtt ccacttggac gcgagggttg acttcgataa
     6781 agtaaaaatc atctccttca acaagaaatt ctactgtacc tgcgttgaca tacccgacat
     6841 atttcatcaa ttggacagct gcctggcaga ttttttgacg ttgctgttca ttcatagaga
     6901 cacaaggcgc tacttctact actttttggt gacggcgctg aacagaacaa tctctttcaa
     6961 aaagatggat gacgtttcca tgcgtatccc cgagaatctg tacttcgata tgcttaggat
     7021 tagcgatgta tttttcaaca tacacttcat cactaccaaa ggctgctttt gcttcgcttt
     7081 tcgcacgttc gtagccctct cttgcactct tttcatcgtg agctacacgc atgccgcgtc
     7141 cgccgccgcc tagagctgcc ttgatcataa tcggataacc gtattgtttc gcaaactcca
     7201 aggcatcgtc tatagaagcg attggtccat ctgtgcctgg aatcgaagca atccccgctt
     7261 cgatagctgc agcttttgct ttgattttat ctccaaaaat atctaaatga tgcagtttgg
     7321 gtccaacaaa aatgatgcct tcttcttcgc accgttgcgc aaaattcaag ttttccgaca
     7381 aaagaccgta acccggatga atagcgtcag ctccgcattc tttcgcgata gaaatgatcc
     7441 cttcaatatc tagatacgca tcaatcggtt tttttccttg tcctactaaa tatgcttcat
     7501 ccgctttgaa acggtgtacg gaatattcat cttcttctgc atagattcca acagttttaa
     7561 ttcctaattc tgtacaagca cgaaatacac gtacagcgat ttcacccctg ttagcaacta
     7621 aaactttttt catcgaacca ctcctacatc tttttttatt ataaacctaa gctcttgcgt
     7681 ttttcatccg cactgatgtt gaggacaaag ccgacacaaa tcgaaagcat cagcaaactg
     7741 gagcctcctt gacttaaaaa tgggaacgtg atccctgtca aaggaataac gccagtaatt
     7801 cctccaagat tgacaaatac ttggatcaaa aacattgcgc cgatcccaat gcacaaaagc
     7861 gaattgaacg gatcttttga acggatacct acaaggacaa tgcgcacaat cataaagaat
     7921 agcaacgcta atatcaaaat ccccataatc actcccagtt cttccactac gattgcataa
     7981 ataaaatcgg tttgtgcttc ttgtaaaaat ccttttttct ggatactgtt acctaatccc
     8041 ctgccaaata gtcctccgtt gaacatggca tagtagccgt tgactagttg atgcccttta
     8101 tttaattcgt ctgagaaagg attttggtaa atagcaaatc gattatagat atactgtaaa
     8161 cgtccgggta agatctttcc gtttgtgata ttgatcagcc agatcgtaaa agtacttaga
     8221 caaaagcctc ctactcctac aatcaaagta tacacataat tgactccgct ggctaaaagc
     8281 agaaccaaaa taatcaaaaa gataactgcc gcattaccaa aatcgggaag aatagcgact
     8341 aatgcagtaa gtccgataac cattgcaagt ggacgtttca cagtcccaag aaaatctttt
     8401 tgtacactgt tttgtctttt agataaagtc agagacaggt accagataga aatgattttt
     8461 aggtattcgg caggttggat cgagaatcct gcaatttgca gccaaccttt cgccccattg
     8521 atctccttac caaaaaacac aacaatcagc agcaaaatcg tcaaaactgc gatggctgcc
     8581 atgatcaaac gctggttttt caaaacatct gttttcattt tataaagtag tgcgatagca
     8641 atcaagctaa gcacccaaaa aatactctgg ttaatgactg atgcggacgg attttgtccg
     8701 ttttctaata atagatacga cgtagaacta tataccatga tcaatcccaa tacacataaa
     8761 attaaatagg gaatcaagat gctatagtct aatagatgcc tttttttcac tttcttaggc
     8821 aagtctattc ctccttgcca acttcttttt tatcataaat ttcttggtaa acctcactat
     8881 ataactgatt caattctcgt tctagctcac ttaacatcag ctggccctcg ttctttccga
     8941 ttactcctat acggacagca aaaccgactt gccgggaaaa gccgtacatc tgtgtatcta
     9001 ccacttcttc gaaggctttg cattgtgaga tacagagact gttgttttga tttcggatta
     9061 atcgtttgat ccgttctgct tcatcattta atacttggat cgcaagtttt ttcgacaaag
     9121 aatccatctg tgcttacctc ccattctaat tccttaatta taacatattt gattttcata
     9181 aggagcaaaa aaaacagatt taaccagttg tttttattca tttttacgta ttcgttcttt
     9241 ctacttatcc agaacgttag agtccgcatt ttttatccga gataaaaagg cttcgccata
     9301 tttatcaagc ttgctttggc caactccttt tatctgaagc atctccaatc tgcttgatgg
     9361 acgtattcgg ctcatttctt tcaatgtgga atctgaaaat acgacatacg ggggaacgcc
     9421 tgcttctgat gctagatccg ttcgcagttc ccgcagcact tcaaatagtg tatcggtttc
     9481 ttcatccgat agctgctgga ctttcttggg ttcctttcga taaacagatt cttgtccttt
     9541 caatacttgt atccctcttt ctgttacttt caaaacagga tactcaccat tgatggtcag
     9601 aagatagcca ttcgatatca aatattcaat taactgcatc gtttcttttt gcgattggtt
     9661 tttcatcagt ccataggtag ataaatgtcc aaaacctaaa gcttgcagct tttgttcttt
     9721 cgatcctgct aagactttca tcagcatttg tttaccatag ttctcaccca tacgttttaa
     9781 gcaagaaaga accatctgtg cttctgtcgt tacttctact atttctctgt catctaaaca
     9841 gttaccacaa cggttgcacg gactgcttgt ctcgccaaaa tagtttagga tgtattgctg
     9901 aagacaggac tcaatataca cgtattctgt catcgctttt aatttttcat attctttttg
     9961 cttttgccct tcttctcttt gggactgctg gataaaaaac ttttggactt ggatatcttg
    10021 aggagagaaa agcaggatgg cttcactgga aagaccatct cttcccgccc gaccagcttc
    10081 ctgatagtat gcttctagag agccaggaat ttgataatgg ataacaaaac ggacattgct
    10141 tttatttata cccataccaa atgcatttgt cgctacaatc agctgcaagc gatcgtataa
    10201 aaaggcttct tgcatttctg ttcgctcatt ctcatttaga ccaccatgat atctacccac
    10261 ggaaaaatcg aattttttaa gcaaatgata gagacggtct acttcttttc tcgttgctgc
    10321 ataaatgata ccagattgat ttttgttgat ctttaaatat tcgattagat attgttcttt
    10381 tttttgatct ttaattactt gaaatcgtag attttctcgt tcaaaaccag tctgaatatg
    10441 gttgttttct ggaattttta aaagtctttt tatgtcagca gctacttgca cagtcgctgt
    10501 agctgtcaaa gcaacaatta caggacgatt tggcagttga tctagctggt tcgccatctg
    10561 taaataactt gggcggaaat catgccccca ctgagagata caatgtgcct cgtcaatagc
    10621 gatcatacgg acgttccatc gcttcattgc ttggataaaa tccggcatga taaaacgttc
    10681 aggagcgata taaagtaaac ggtactgctg tctatcgatt tcttggaagc gtctagctgt
    10741 ttcgtaacca tctaacgagc tattgataaa agtggcgggt atacctaatt gattcgccgc
    10801 gtccacctga tccttcatca aagagattaa aggggacaca accacagtaa gcccatctaa
    10861 tagtaaagct ggaagctggt aacagattga tttcccactt ccggtaggca tgattcctaa
    10921 tacattttcc tgatcaatga ctttctggat aatttctttt tgaccaggtc taaactcatc
    10981 atagccaaag tattgtttta ataagtcgtt catcttcccg ctcctttctc ttgccatttt
    11041 acttcgattt tcttcgaagg acaagttgat ctcctttcgt gaaacattat cttattatga
    11101 aacaaaaact ctctagaaat aagtttttta aaagtctttt aaacgtcttt cggcgtattt
    11161 tctgaattta tgtccattga caaagatgac ttctccacag aaaaga
//
LOCUS       DACCPY010000094        10837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCPY010000094 DACCPY010000000
VERSION     DACCPY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.097"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            42..1472
                     /locus_tag="IVO69_002269"
     CDS             42..1472
                     /locus_tag="IVO69_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAP7401023.1"
                     /translation="MCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRR
                     FQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRL
                     AYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDS
                     TTHEIILSFLGKVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPN
                     PFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDC
                     KICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAY
                     DDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRNDLTFFTNGRSVCLTELKGYQVT
                     TGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1587..1637
                     /locus_tag="IVO69_002270"
                     /pseudo
     CDS             1587..1637
                     /locus_tag="IVO69_002270"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1671..3047
                     /gene="tet(L)"
                     /locus_tag="IVO69_002271"
     CDS             1671..3047
                     /gene="tet(L)"
                     /locus_tag="IVO69_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7401024.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTALYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FPILILARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTIVSSSLKQKEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNMLFLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3611..4873
                     /locus_tag="IVO69_002272"
     CDS             3611..4873
                     /locus_tag="IVO69_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7401025.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5118..5657
                     /locus_tag="IVO69_002273"
     CDS             5118..5657
                     /locus_tag="IVO69_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7401026.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHS"
     gene            complement(5650..6621)
                     /locus_tag="IVO69_002274"
                     /pseudo
     CDS             complement(5650..6621)
                     /locus_tag="IVO69_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            complement(6812..8131)
                     /locus_tag="IVO69_002275"
     CDS             complement(6812..8131)
                     /locus_tag="IVO69_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HAP7401027.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            complement(8359..8886)
                     /locus_tag="IVO69_002276"
     CDS             complement(8359..8886)
                     /locus_tag="IVO69_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7401028.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            complement(8930..9793)
                     /locus_tag="IVO69_002277"
     CDS             complement(8930..9793)
                     /locus_tag="IVO69_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7401029.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            complement(9826..10560)
                     /locus_tag="IVO69_002278"
     CDS             complement(9826..10560)
                     /locus_tag="IVO69_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7401030.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(10541..>10837)
                     /locus_tag="IVO69_002279"
     CDS             complement(10541..>10837)
                     /locus_tag="IVO69_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7401031.1"
                     /translation="KYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKP
                     EKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI"
BASE COUNT         3441 a         1868 c         1997 g         3531 t
ORIGIN      
        1 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
       61 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
      181 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
      241 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
      301 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
      361 tttatggcgg aatcctgcat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
      421 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
      481 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
      541 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
      601 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
      661 acagtgaccc gcttctacaa tattatgtgg attctacgac acatgaaatt atactttctt
      721 tcttagggaa agtacaaatg gaagtgactt gtgctctatt gcaagaaaag tatcatgtgg
      781 aggtaaaaat aaaaaagcct acagtcattt atatggaaag accgttaaaa aaagcagagt
      841 ataccattca catcgaagtg ccaccgaatc ccttctgggc ttccattggt ttatctgtat
      901 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
      961 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
     1021 atggttggaa tgtgacggat tgtaaaatct gttttaagta tggcttatac tatagccctg
     1081 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gttttaaaaa
     1141 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgca ccacaagaat
     1201 atctttcacg agcatatgac gatgctccta aatattgtgc gaacatcgta gacactcaac
     1261 tgaaaaataa tgaggtcatt cttagtggag aaattcctgc tcggtgtatt caagaatatc
     1321 gtaatgattt aactttcttt acaaatggac gtagcgtttg tttaacagag ttaaaaggat
     1381 atcaggttac cactggcgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     1441 aagtacgata tatgttcaat aaaataactt agcacatttt atattgttat ataaatgtgg
     1501 cttcttgtta aacgaaacga gatgaaatat tttttaataa agatttgaat taaagtgtaa
     1561 aggaggaatt aattatgaag taaaatgaat gtaacagggt tcaattaaaa gagggaagcg
     1621 tatcattaac cctataaact acgtctgccc tcattattgg agggtgaaat gtgaatacat
     1681 cctattcaca atcgaattta cgacacaacc aaattttaat ttggctttgc attttatctt
     1741 tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt
     1801 ttaataaacc accggcgagt acaaactggg tgaacacagc ctttatgtta accttttcta
     1861 ttggaacagc tctatatgga aagctatctg atcagctagg cattaaaagg ttactcctat
     1921 ttgggattat aataaattgc ttcggatcgg taattgggtt tgttggacat tctttctttc
     1981 ccatacttat tctggctcga tttattcaag gagctggtgc agctgcattt ccagcactcg
     2041 tgatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta
     2101 ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc
     2161 attatattca ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt
     2221 ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa
     2281 ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt
     2341 cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa
     2401 cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt
     2461 gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga
     2521 aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa
     2581 tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat
     2641 acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct gccttttttt
     2701 tagaagtaac accatggtta ctgacaatta tattagtttt tgtttttggt gggctgtcat
     2761 tcaccaaaac agttatatct acaattgttt caagtagttt gaaacaaaag gaagctggtg
     2821 ccggaatgag tttgcttaat tttaccagct ttttatcaga gggaacaggt attgcaattg
     2881 ttggtggttt attatctgta cgcttgctga atcagaagtt gctacctatg gaccttgatc
     2941 aatccactta cttgtatagt aatatgctat tccttttttc aggaatcatt gtcattagtt
     3001 ggctggttac cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat
     3061 caactttggg agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt
     3121 gttccattaa agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt
     3181 ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc
     3241 ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt
     3301 ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt
     3361 tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt
     3421 gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac
     3481 tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta
     3541 cttggaagtg gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag
     3601 cgatttacat atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa
     3661 aggcatgcaa tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca
     3721 tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg
     3781 tgtcaaagaa attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt
     3841 tcttgtaaat gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg
     3901 agaacaaaaa cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca
     3961 aaatattgct tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt
     4021 tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt
     4081 atggctacaa gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg
     4141 tgaacgtggc tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga
     4201 aaaagagatt gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta
     4261 tagcgataaa gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga
     4321 agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa
     4381 gaaaccaacc aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc
     4441 gagagataac gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag
     4501 agaatataaa aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt
     4561 agagcgattt aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt
     4621 aaagtctaaa ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga
     4681 attccttaag gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt
     4741 agacaaggta aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa
     4801 gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg
     4861 aatgagttta taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac
     4921 ttgttctttc ttatcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa
     4981 ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt
     5041 gcacagaaaa accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg
     5101 gggtttcttt taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg
     5161 ttttagtgga caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta
     5221 atgttgatta ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag
     5281 attgtgctga aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc
     5341 gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac
     5401 atggcattca gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt
     5461 ggttgtttct cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt
     5521 tgtcagatat ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc
     5581 ttgttggttt tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc
     5641 agcacatgca ttcttaatca cttggatata ttctttttgt tttaacgctt caataatact
     5701 tgctcttgtc gcttctgttc cgatcccctc gacttcttgt aaaatcttga ttgcttcttc
     5761 atcatcaacc gttttattag cggttttcat agcagttaat aatgtaccct ctgtaaaagc
     5821 tttcggtggt tgtgtttctt tttcggcact cttaacgtca acctcggcat gttctccaat
     5881 gataaccaag ggtaacgttt gaacgtcctc tttttcttct ttggtttgtt gtttgaaaag
     5941 aattttccaa ccttcttgtt ttggtgtctt tccgatcgat tgaaacagca aatcagctac
     6001 tttcgtttgt atcttggttt cttcatacaa gtaatcaggt aaaaacatgg caacggtcgt
     6061 tttaacgact aaagcatata tttttcgttg taaatcgtcc atttttgcta atgcagattc
     6121 ggtaggtact tgttttgttg ggataatggc gtggtgttcc tgtaccttac taccgtccac
     6181 ataacgcttt ctaggctctg tttgaaccat ttcgagatca agtcctaaaa aaccgctata
     6241 tttgccaaaa tttgctttta aataagcaaa ttcgttctct gtaataaatg gtgtatctgt
     6301 tcttggataa ctcaataatt ttgcttcata cagtccttgc atagctttta aagtttggct
     6361 tgctgtcgct ttataaagct gattgacttt tgattgtaaa ctacttaaag aaaacaaact
     6421 cggactattc gttttcttct ctttggtttg aacatcagca attatcccct cttgattgcc
     6481 tattttagct tgtttagaag aaacaaagga taaaagttcc tcttggctct taaaacgctg
     6541 tgtggggctt agaacgccct taaacgaccc ttggtttact tttatactag cttccacctc
     6601 gaaaaaacct gaataattca tagctctaaa tttaacctgt ttttttgaac aaattgccaa
     6661 tttttaaatt aggcagatac tttcttccaa aataatgtgt tttcgaacag gatgcctacg
     6721 gaagaagatc ataataaaaa ttatttgtct atttaagggc agacgaatcc cttgaaaaag
     6781 aatcggttaa aaaatttgtg tgataactag tctaaatcca ttgtttggat cgccgcaggc
     6841 gttcaaaaac cttataaaat tcagtttgat acacatgata acttgacagt ttgagataga
     6901 cctgtctccc agtctgaaca agtttaccgg ctactttaag caatatcagc cggatggaat
     6961 gaatcgtcat gccctggttc actttatcaa aaccaatgct ttttaaaaaa ttaacgaggt
     7021 tgtatgcaag gacactgatc atcattctga catgattttc taagaaacgc gggctatctg
     7081 ttttatcgaa gtaaaagcca cctttcgctt ctttgatgaa attttccatt gttcctcgct
     7141 tgccgtaaag agaaaatatg acttcaggcg agacattgtc ggatagatta gtcacaataa
     7201 acgcatgatg aaatagcaat tctcctcctt cacggacgga gcgtatacag acacgacgag
     7261 gtttcgacca ggattgtgct tgataaggta gtgaaaaata ctgcacttct cgctcttccc
     7321 attcttgatt gtccccgtaa agaacagaat gttcagctaa ctgacttaac ctccgattgc
     7381 tctttaatcg gataacgtac tggctttcag ttgcttcaca agactcatac acctctggtg
     7441 tggcgaagcc gctgtcaccc cgaaccaata tctcggtatg aggtaacgtg ctcttgtagt
     7501 ggtgtaaaag cgggttgata aaggctttta cccctttaga tgtgtactga ttgcctgaac
     7561 gcagttcagc ttttaagaaa tctccggtca gtccatcaaa ggcaactaat gggtgatagc
     7621 cgtaggtttg ataatgggca ttataatctg tttgttcttg gcgtccaaag gtatccgaat
     7681 gtgtggaatc gacatcaatg attaactcgg tgtcattgcg aattaaacgt gctttatcaa
     7741 tcaatgactg atttaatgct tggagttgat cgatgttctc ctcggtgaaa cgatccagaa
     7801 accgtgagat tgaggattgt gatgccaact gttttttacc gagtacagct tgaaagacag
     7861 gatcccgtct taatagatta gctgatgagt ctgccgagta accagcaatt aattgcataa
     7921 taagctgttc caatatcgct aagttatcat gggtaaaata agcccgatga tctgtaatat
     7981 ggagccattg tttagctaag tgcgaaaagc cgaaggtatt cattaattct ttaaccagga
     8041 ctaaccccga atcacttgat agacgaccac cggtatgtga aatagtgata tttgaattga
     8101 atttaacttg atttttgtgt aagctagtca tgagaagaac tcctttctta tggttggttt
     8161 cgtcaccttt accatagcag aatggggttc ttttttcatc acttaagggg tgaaactaaa
     8221 aaagtaatta tcagactgcc gttaggcagt ttcagacggt ttcaattaaa aagtatgaat
     8281 tattcagggt aaacatcttt cgttgtattg taaattaacg ccatccaaaa ttttgattca
     8341 agattttctt tcggtttatt atatgtttga gccaatataa gctacaaaac tgctgtctat
     8401 ccatttttga gtaacctcat ttatatcaat cccaatagta gccaacccaa taatgctgca
     8461 atccaatttc tctaacaaag ctattaaagc cttcatttga gaacctgtct ctatccaatc
     8521 atctacaatc aaaactcttt tgttcctcac aatagaactc ttagatattt caaagctttt
     8581 atcttgacct gaatagtcgg taaaatgtgt cgaaataatc tcttcttttg cgtatggcag
     8641 cttatcacct tttcttactc caataaaccc cactccaagt tctttggcta tggcagtgcc
     8701 tagtatccat ccaattgctt ctggggcagc cacataatct actttatttt gaaaatccaa
     8761 tgatagtact tttacaattt tatcaaaaac ggttttatgt gtaaaaatag ttagtaaatc
     8821 atattttcca attgagttac gaggtaatat acctctaatt tcatttataa catcagtact
     8881 ttccatacat ttacacccct tgcttatggt taataatctt tgctaatctt taaaaatagt
     8941 cattttcaac atatttttta tacatgtcct cggtatatct tgttatgttc ttaccatact
     9001 ctggataatc aaaccccagt agttctgcta cctctttgga cacttccctg aacaattggt
     9061 ggcatataaa taatgacttc caaatatttt cataggaatc catgcgatat gtagataata
     9121 atctattcca tagatcttca tcaatgtatt tgttaatata cttataattt ttcccaacac
     9181 ttaatgaaaa ttctgtcttt atcccaacct tccacgacat catcctaagt agttcaaacc
     9241 gtagaatctg gttcagatga tcgattgcaa acagtatctc tttgcggcac aatcctttaa
     9301 taacataagg tgttacattc caaaattcat tgcagcaatc atcatactcc cttgcgcttg
     9361 gctttcttac atgataatct atatcagtcg gaactatgtc ccttttaatt ctacaatctt
     9421 tatcaattag aacctttatt aatttatcgc cctttaggta attatctaac tcttccaagg
     9481 gcaataaggt aagatcaatt ttattgtaat catcaaatag cataagatag gaaaatccct
     9541 tttcttcagg tgggaataat tccatatcct ccggcttttg catcattatt atattcccaa
     9601 attgattaag ccagtcatca ttagatataa acggttctat atcacttaca aaatatgtaa
     9661 tatcataatc ctgaaattca tctttaggta tattaatatt tgcgcgtgac ccctcaaggg
     9721 tcacaattcg aatacgttca tcctgttctg ctaaagaaag tactaaatcc atcatttctt
     9781 tttctgatct catttacata ctcctcgttt attttttcta tattattaca tcttcttttt
     9841 tgccgataca atcagcatca ttgggcgtcg catttcatcc gccatccccg gaatatccat
     9901 catgttctct ggcggctgtg gctccacaat ctgatttatt ataaaactat ttgaaagcag
     9961 tgtatttaga tatgtggtca gtgttctatg atattttgta accttttctt ccaaaaacat
    10021 agctgtccgt ttgccctcat aataataatt gtccaccggg aaatgcagta tttctccttt
    10081 ttcgttataa taccagtctt gtgttccatg agcagtaaaa acaggatgtt caactgtaaa
    10141 aactaaattg ccaccagcct tcagcatcct atatatcttt tttattaaat tctcatagtc
    10201 tgctacataa tgaaacgcaa gcgaacttag tattacatca aagctctcct ctgggaaatc
    10261 cacatcttct atggcacagc attcatattc aatctgtgga aaatgggttt ttccttttgc
    10321 tacttcgagc attttatgag aaatatcaac acctactaca gaggaagcac cgttttccat
    10381 cgcatatata cagtgccatc catagccgca tcctaaatca agcacacgct tacccttaaa
    10441 atcaggtagc atctttttca aagtctccca ttctcccgca ccagccagtc ctttctgcga
    10501 gcgactcatt tgactgtatt tttgaaaaaa tatattatca tcatatttgt tttctttcat
    10561 ctgaactctc ctcgtttaaa aagttattta tctccgatac aatttcattc acttctttat
    10621 aaagtttctc ggtcatatcg tagcattcaa aaagagaaag accgagtact tcaaaaatat
    10681 gattaactct ttcggcatat ttttcaggtt tatattcaaa agtttcaagc agttttatag
    10741 ctttcttttc gttaatgcaa taagcattat tacatgcaaa tagtacttga tttaagcatg
    10801 aaataatacg aaaaacatgg cctgcaatat aatattt
//
LOCUS       DACCPY010000095        10496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCPY010000095 DACCPY010000000
VERSION     DACCPY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.098"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..360)
                     /locus_tag="IVO69_002280"
     CDS             complement(<1..360)
                     /locus_tag="IVO69_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7401032.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IVO69_002281"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IVO69_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7401033.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IVO69_002282"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IVO69_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7401034.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IVO69_002283"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IVO69_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7401035.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2240..2872
                     /locus_tag="IVO69_002284"
     CDS             2240..2872
                     /locus_tag="IVO69_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7401036.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="IVO69_002285"
     CDS             complement(2874..3779)
                     /locus_tag="IVO69_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7401037.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4517)
                     /locus_tag="IVO69_002286"
     CDS             complement(3864..4517)
                     /locus_tag="IVO69_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7401038.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="IVO69_002287"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="IVO69_002287"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7401039.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="IVO69_002288"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="IVO69_002288"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7401040.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="IVO69_002289"
     CDS             6766..7851
                     /locus_tag="IVO69_002289"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7401041.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="IVO69_002290"
     CDS             7848..8912
                     /locus_tag="IVO69_002290"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7401042.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="IVO69_002291"
     CDS             complement(9161..9964)
                     /locus_tag="IVO69_002291"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7401043.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IVO69_002292"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IVO69_002292"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="IVO69_002293"
     CDS             complement(10271..>10496)
                     /locus_tag="IVO69_002293"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7401044.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DACCPY010000096        10443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCPY010000096 DACCPY010000000
VERSION     DACCPY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.099"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(22..339)
                     /locus_tag="IVO69_002294"
     CDS             complement(22..339)
                     /locus_tag="IVO69_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401045.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            complement(780..1643)
                     /locus_tag="IVO69_002295"
     CDS             complement(780..1643)
                     /locus_tag="IVO69_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7401046.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            complement(1645..1917)
                     /locus_tag="IVO69_002296"
     CDS             complement(1645..1917)
                     /locus_tag="IVO69_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7401047.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(1934..2149)
                     /locus_tag="IVO69_002297"
     CDS             complement(1934..2149)
                     /locus_tag="IVO69_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7401048.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            complement(2248..3102)
                     /locus_tag="IVO69_002298"
     CDS             complement(2248..3102)
                     /locus_tag="IVO69_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7401049.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            complement(3240..5384)
                     /gene="topB"
                     /locus_tag="IVO69_002299"
     CDS             complement(3240..5384)
                     /gene="topB"
                     /locus_tag="IVO69_002299"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7401050.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPIFADETFIT
                     FGFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVATDKKKQFKIVAELLKQANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTYKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKSQEELLSFVSSKQAKIGNQEGRIADV
                     QTKEKKTNSPNLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITD
                     SEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGERAEVDVKSVEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFINNIKKFIVHLLEAVPTDIEKLSFSDYQEQKEKE
                     AEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKTKDKKSYNAVVKIGEKGYIDFVSFSK"
     gene            complement(5384..6001)
                     /locus_tag="IVO69_002300"
     CDS             complement(5384..6001)
                     /locus_tag="IVO69_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401051.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLKHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKNNEKRDS"
     gene            complement(6533..8023)
                     /locus_tag="IVO69_002301"
     CDS             complement(6533..8023)
                     /locus_tag="IVO69_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7401052.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKRFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8415..8663)
                     /locus_tag="IVO69_002302"
     CDS             complement(8415..8663)
                     /locus_tag="IVO69_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401053.1"
                     /translation="MELAFRSRLRQMRGNKTRKQFAEEIGMKESNYLKIELGKSNPTV
                     KTLERIAKLTNSTLVIDLIPNDSEQLELQLDSEISKKE"
     gene            complement(9135..9872)
                     /gene="erm(B)"
                     /locus_tag="IVO69_002303"
     CDS             complement(9135..9872)
                     /gene="erm(B)"
                     /locus_tag="IVO69_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7401054.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(9997..10080)
                     /locus_tag="IVO69_002304"
     CDS             complement(9997..10080)
                     /locus_tag="IVO69_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7401055.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYVDK"
     gene            complement(10101..10212)
                     /locus_tag="IVO69_002305"
                     /pseudo
     CDS             complement(10101..10212)
                     /locus_tag="IVO69_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
BASE COUNT         2924 a         1935 c         1613 g         3971 t
ORIGIN      
        1 tttttttgct aggcttttct tttacatttc tttaattttc aaatttttta tttcccactc
       61 aaaccattcg ctaaaatctt gtttaaagcc attaatagaa tattgctctt gttcttctgc
      121 ttctttatcg ttttgagggt ctaagtaggt cggtttatct tttggtcttg cgtatacacc
      181 gatccatgtt ttaaaatcgc cctcaaaact gctatctatg ataaagcctt gctcttttag
      241 ctcttctctt acttgagctt ttagatattg ctgttcttgt gttccataaa actcattttc
      301 tgtaaaatct tctaattgta atttttttgt tttttccatt ttcttttcct cttttctttt
      361 ttttgatttt tagttttttg acaaccattt ttgttaggcg tttgaggaag tgaagctgtt
      421 ttcctcatgt tttaaaagct agaaataaat tacagtgtac gctcctttct tttttcgttt
      481 tgttggttca ttttcgccag tgtgttcgtg tcgtttgctg tgacgagaac gcaacataga
      541 cacaaagaaa acacaagggc gagcttgctc gtttatatac ccttgtgttt tcgactggat
      601 atgttaaggt aacaaacagt aaatgacacg ttacagactg caaaatgtac caactgaaaa
      661 aagaaagaat accgttgtgt gctttatttc tcgatcagct tttaaaacat gaggaaatgt
      721 ttttataaaa aaagagtagt taccgttttt tggtaactac tcttttcctt ttttaaggtt
      781 taaagtcctg gaagtttagg tgttttgggt attggtggtg taggtggctg caagctttcc
      841 attttttgtt gaattgcttt aaattcaggg gtttcttggt gttgattttg aagcattttt
      901 tgctctattc tctccaaagt cggttgaatt tctttccctg atactttacg atttaattct
      961 ctttctaagg tctctttcgg actaatggaa ggcgtttcta agcttgaata gagtttcact
     1021 ccttctctat tgtaaagcct tatatcgcta aataaacccg ttttatgaag ggtttctaaa
     1081 ttggtcggta agtttttgac aacaatatca tgcgcttgtt ttggtgttgc cctggctgtc
     1141 attggatcat ctgcatacat agtttcatat cgttcaattg ttcctaaata tgagttgatt
     1201 ttaggtactg ccatgacata catttttgtt tcataacctt tggcttgaag cattgttgcg
     1261 gtttgaatag gaacgtctgt tgttcgtcct gtaccttcga tcaccaaatt atacccttga
     1321 tcgctcaaac ggcttatgat cgcttctgtc atgcgattag aataaggggt gacgtgtttt
     1381 actacgtctt tttcataaag tttcactagt tcatcaaaat tagggtgctg ttgtttaaag
     1441 gtatcattat caatgacaat aacattccct tgtgtttctt caagaattgc tgatcgcaaa
     1501 ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag cggttggcga ttcaaccgct
     1561 ttttttcctt gaatcaattc ttctaaatta tcatttaagc gattctcaaa ttgtttgtca
     1621 gtaaaattga ctatatttgc catattaagc cactttctct ttattcaaaa cttgttttga
     1681 gtaacgattc attgaccgcc aatactcatg aacggcttgt aattcttcta aagaataatc
     1741 aaaaagattt acacgttttg caagctttaa ttggtttaat aaattgactt ccaataattg
     1801 agagtcattt ttatttaatt catggttcaa aacatagttt aatactcgat tataaacact
     1861 tgccgataac tcattaatga tttcaatttc aaatgttttt tcatatgtaa ctgccatttc
     1921 attttcactc cttttaaagt ttgtcaggta aatatttccg aatatattct tctaaggctt
     1981 tatccataac ttcttttacg tttccgccat tctttgctgt ttcgattttt atgatatggt
     2041 gcaagtcagc acgaacacga accgtcttat ctcccattat atcttttttt gcactgattg
     2101 gtgtatcatt tcgttttgct ttttgtgcgc ctaaatttcc cacaatcact cacttctttc
     2161 tatttcttct tattcttatt ttatcatcaa caatcacaaa tcacaagtga tttgtgattt
     2221 gtgattaatc acttgtgatt tgtgattcta ttctttttcg ttttctaatt gaataattcg
     2281 ctcaagcatt tcaaaaaata cgttctcata catggataaa acttttttgt catagccttt
     2341 gtgttctgta attccgtttt tagaccaggt acttacttta ttacttcgct tgataatatt
     2401 ttggaaaacc aagttatctt ctttatgttc tttgtacagt tcttccaggt ttgattttat
     2461 cgttgcgctg tccgtatcaa ctaaataagg aacaaaaccg atcatatcta gtccagggtt
     2521 aaattgttct tgtaaatcaa tcaaatagga aatatagttt tgaatgttgt ttgtactttc
     2581 ttcttctgct tgtaaaggga tcataacgta atcacttgcc acgattgcat tatttgtata
     2641 aacgcttggc gttggtacag tatcaataat aatgagatca tagtcacttt ttaaaggtgc
     2701 taaaagagta gcaagcaatc tactttcgtt ttcaaacgtc catgagcgag ttaattttgg
     2761 cagtaacatc aaatcaaacg tgccaggaat caagtctaaa ttatcagtca aatgaataat
     2821 agaagaagcc aagtttccat ttttcagtcc ttcataaaaa ttgacacgtg gcaattctac
     2881 tttaaacgtt tttgctaagt cttttgtcaa tgttgcttgt aagtccttat cgatcattaa
     2941 aacttttaaa ttaaatttgt ctgtcaagta agcaaacata gtagataatt ttgactttcc
     3001 aacaccacct ttaaagtaat tattcaagat aacaattgct tcatttttac tgtttaaaat
     3061 acgtcttaat tcttcaagta ttttgagttc ttccttctcc ataactacgc cccattcttt
     3121 ttttgtatag taatattgta tcattaagaa tcacaaatca caagtgatta atcacttgtg
     3181 atttgtgata agtgatttgt gattaatata taaaagccct gtttaaaggg cttttatgct
     3241 tattttgaaa atgagacaaa atcaatatat cccttttctc cgatttttac aacggcattg
     3301 taggactttt tatctttcgt tttgattcct tttaccaggg tttcttttcc ctctagtaat
     3361 tcttttacat ttgttttggt tagttttttc tttctaaaat gttcagctaa agtaaacttg
     3421 cattcaggat aattcgaaca accataaaac gattttttta atacaatatt gttgccacac
     3481 ttaggacatt tgcctacaat acttttttct gcttcttttt ctttctgttc ctggtaatca
     3541 gaaaaactta atttctctat atcggtaggt acagcttcca gtaaatgaac gataaacttc
     3601 ttgatattat taataaagtt ttcttgattg ccttctcttt taccaatctt ctttaaatac
     3661 gtttcccatt tagccgtcat ttcagcactc gttaaaaggt gctgactttc aactgcctgg
     3721 cacaataatt ttcctttttc agtcacaaca agtttatttt taatgacttg gatatattct
     3781 ttttgtttca aggcttcaat aatgcttgct cttgtcgctt ctgttccgat cccctcgact
     3841 tcttgtaaaa tcttgattgc ttcttcatca tcaaccgttt tattagcggt tttcatagca
     3901 gttaataatg taccctctgt aaaagctttt ggcggttgcg tttctttttc aacactctta
     3961 acatcaactt cggcacgttc gccaatgata acgagtggta acgtttgaac gtcctctttt
     4021 tcttctttgg tttgttgttt gaaaagaatt ttccaccctt cttgttttgg tgtctttccg
     4081 atcgattgaa acagcaaatc agctactttc gtttgtatct tggtttcttc atacaagtag
     4141 tcaggtaaaa acatggcaac ggtcgtttta acgactaaag cataaatttt tcgttgtaaa
     4201 tcgtccattt tcgctaatgc agattcggta ggtacttgtt ttgttgggat aatggcgtgg
     4261 tgttcctgta ccttactacc gtccacataa cgctttctag gctctgtttg aaccatttca
     4321 agatcaagtc ctaaaaaacc gctatatttg ccaaaattcg cttttaaata cgcaaattcg
     4381 ctgtccgtaa taaatggcgt gtctgttctt ggataactca ataatttagc ttcatataag
     4441 ccttgcatag cttttaatgt ttggctcgct gtcgctttat aaagctgatt gacttttgac
     4501 tgcaaactac ttaaagaaaa cagattagga ctattcgttt ttttctcttt ggtttgcacg
     4561 tcagcgattc tgccctcttg attgcctatt ttagcttgtt tagaagaaac aaaggataaa
     4621 agttcctctt ggctcttaaa acgctgtgtg gggcttagaa cgcccttaaa cgacccttgg
     4681 tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaagtt ttctattgct
     4741 tcctggcgtt gataaatgag atataaagtc ggtgtttgta cacgtcctaa tgaaaatgta
     4801 ccttgtacgc ctttttgttg taaattcaag gtatataaag ggcttgcgtt catgccgatc
     4861 aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta attcattcct
     4921 ggttgcaaat tttgaaaacc gctacggatc acatcttttt ctaaactatt gatccatagt
     4981 cttttatacg ttttatcttt agaaaaggca tttgctttat gaatgatcga ccaggcaatg
     5041 ttttcgcctt ctctgtcgct atctgttgcg acaatgattg tatttgcttg ttttaaaagt
     5101 tcagcaacaa ttttaaactg ctttttttta tctgttgcca cttcaaaatc gtatcgatca
     5161 ggaaaaatcg gcaatgattc aagtttccaa ttttgccact tttcgtcata atgacctggt
     5221 tctgctaact cgactaaatg cccaaaacca aacgtgataa acgtttcatc tgcaaagatt
     5281 gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc acttgcatag
     5341 gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta tccctctttt
     5401 cattgttttt tctttgatcg actgtcacgt tatatcttga tcgatacctt ctaaacgttc
     5461 ggcgattaat tccagtttgt tcttcaactt ctttatcgga acaaccattc aaaaataaat
     5521 caaaagcatg ttttaaacgt ggatcatttt ctttgaattt aggttgtcgc cctttgaatt
     5581 ttcctttttt cttagcaatt tcaattcctt gggcttggcg ttctttaatt cgtttgcgtt
     5641 cagattccgc ttgatactta tacaattcaa tcactaaatt attaatcagc cgtcttaaat
     5701 tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt gcccccttaa
     5761 tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat cgatctaatt
     5821 cagtaacaac aactatatcc ccttcacgaa tatagttaag catagcttgt aattgtgggc
     5881 gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc tgtaacgctt
     5941 gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca attttcgcca
     6001 ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagctat atctactttt
     6061 tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc ttgcgcatat
     6121 tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata aataaatttt
     6181 ttcattttac tcctcctatt atgctcaact taaatgacct attcaccaag tcaattatac
     6241 tgctaaaacc atattaggac aaataagtat actctattga cctataaatg atagcaactt
     6301 aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag cctttccctc
     6361 acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc tccccctaat
     6421 aattaattaa aatcttactc tctatatttc tgctaatcat tcactaaaca gcaaagaaaa
     6481 aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat tttcagcctg
     6541 tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct agcttgttta
     6601 aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc tttatatagc
     6661 tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta acactagcaa
     6721 gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga ttcgccttca
     6781 gtaccttcag caatttatct aatgtccgct caggaatgcc tagcgcttct ctaatctctt
     6841 ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata taagccatta
     6901 aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt ttcttgaatt
     6961 taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat acccaagctt
     7021 tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa taggcacttc
     7081 taacaatttt gattacttct ttttcttcta agggttgatc tagtcgatta ttaaactcaa
     7141 acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct aaagagaggg
     7201 taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc gtttcgtgca
     7261 ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg cttaaaaccg
     7321 ttagacttga acgagtaaag cgcttattat ctgtttgttt gaaagaccaa tcttgccatt
     7381 ctttgaaaga ataacggtaa ttgggatcaa aaaattctac attgtccgtt cttggtatac
     7441 gagcaatccc aaaatgattg cacgttagat caactggcaa agactttcca aaatattctc
     7501 ggatattttg cgagattatt ttggctgctt tgacagattt aaattctgat tttgaagtca
     7561 catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat ttgataatca
     7621 acgtaggcat aaaacctaaa tcaatcgctg ttgttaaaat atcgcttgct gaaattgttt
     7681 cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt aaattatttt
     7741 ctgaatgacc tttcgtgtat gaacggtttt catctgcata cgtaccataa cgataaacgt
     7801 ttggtgtcca atgcgtaaat gtatcttgat tttcgtgaat cgcttcttcg gaagtcagaa
     7861 caacgccacg tccgccaatc atgcttttct ttgagcgata cgcaaaaata gcccctttgc
     7921 ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg tactttaaca
     7981 agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg ttctcctttc
     8041 ttacgaaaat taattagtta gaagctacga tcaaagttga atcacaacaa aaaaggcaat
     8101 caactaagtt tttcttaatt gattgcctgg tatcttctta aagacttgaa attccctcaa
     8161 aaacccgata taatgggttt acagatattt aagtatctga ttaataaagt aattaaatac
     8221 tttaccaaat tttgggtctc gacttcttta attgattggt ggtaatcaat taaggctcgc
     8281 aacttatttt cttggcggaa aataagttgg tcgtggctct ttttttgtat tctttattca
     8341 gttcgttgtt tcgttatatc tagtataccg cttttaaaaa aaataagcaa ttaaaaagtg
     8401 taaaaatatt acgtttactc ttttttgctt atttcgctat ctagttgtaa ttctagctgt
     8461 tccgaatcgt tagggatcaa atcaatgacc agggttgaat ttgtaagttt tgcaatccgt
     8521 tccagtgttt ttacagtcgg gtttgatttt cctaactcaa tttttaagta attagattct
     8581 ttcattccaa tttcttcagc aaactgttta cgtgttttat ttcctctcat ttgtcttaaa
     8641 cgacttctga atgctagctc caagtttctc ccccctcttt ttgtgtaata aaattcacgc
     8701 tttattttaa attatagctt ttaatatctt aataaacaag tgattaatca cttgtgattt
     8761 gtgattaatc acttgtgatt tgtgattatt ccaaaagtag gtaatttcac caaccttttt
     8821 caaaagtttt tatcgacgat tattggactg gatcgtgttt cctaaagcga ttctaagcgc
     8881 tcgggacccc atttagaaaa agcaggaaaa tgaaatggta ccataaacgg tcgtttatgg
     8941 taccattcaa atttatcctt attgtacaaa ataacagcga aatttttaaa tctattcctt
     9001 atcgatacaa attccccgta ggcgctaggg acctctttag ctccttggaa gctgtcagta
     9061 gtatacctaa taatttatct acattccctt tagtaacgtg taactttcca aatttacaaa
     9121 agcgactcat agaattattt cctcccgtta aataatagat aactattaaa aatagacaat
     9181 acttgctcat aagtaacggt acttaaattg tttactttgg cgtgtttcat tgcttgatga
     9241 aactgatttt tagtaaacag ttgacgatat tctcgattga cccattttga aacaaagtac
     9301 gtatatagct tccaatattt atctggaaca tctgtggtat ggcgggtaag ttttattaag
     9361 acactgttta cttttggttt aggatgaaag cattccgctg gcagcttaag caattgctga
     9421 atcgagactt gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa ggtacgcttg
     9481 tagaatcctt cttcaacaat cagatagatg tcagacgcat ggctttcaaa aaccactttt
     9541 ttaataattt gtgtgcttaa atggtaagga atattcccaa caattttata cctctgtttg
     9601 ttagggaatt gaaactgtag aatatcttgg tgaattaaag tgacacgaat gttcagtttt
     9661 aatttttctg acgataagtt gaatagatga ctgtctaatt caatagacgt tacctgttta
     9721 cttattttag ccagtttcgt cgttaaatgc cctttacctg ttccaatttc gtaaacggta
     9781 tcggtttctt ttaaattcaa ttgttttatt atttggttga gtactttttc actcgttaaa
     9841 aagttttgag aatattttat atttttgttc atgtaatcac tcctgaagtg attacatcta
     9901 taaataaata cagaagttaa acgatttgtt tgtaatttta gttatctgtt taaaaagtca
     9961 taagattagt cactggtagg aattaatcta acgtatttat ttatctacgt aatcactgtt
    10021 tttagtctgt ttcaaaacag tagatgtttt atctacatta cgcatttgga ataccaacat
    10081 gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt
    10141 atacaaaatt ttaagataat gcactatcaa cacactctta agtttgcttc taagtcttat
    10201 ttccataact ttagggttaa ccatacgcaa gaccaatcac tctcggacaa tactcatgat
    10261 tttaatgata aaatccatgt taaacccata gataagaaat acacctgcaa taaccgttac
    10321 ctgtttgtgc caatttaaaa atgcaccact tttttataat taaaacggtt gaaaactgta
    10381 ccactaataa ctcacaatag agagatgtca ccgtcaagtt aaatgtacaa aataacagcg
    10441 aaa
//
LOCUS       DACCPY010000097        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCPY010000097 DACCPY010000000
VERSION     DACCPY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.100"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IVO69_002306"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IVO69_002306"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7401056.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IVO69_002307"
     CDS             1007..1927
                     /locus_tag="IVO69_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7401057.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IVO69_002308"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IVO69_002308"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7401058.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IVO69_002309"
     CDS             3233..4711
                     /locus_tag="IVO69_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7401059.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IVO69_002310"
     CDS             4988..7048
                     /locus_tag="IVO69_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401060.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IVO69_002311"
     CDS             7131..7700
                     /locus_tag="IVO69_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401061.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IVO69_002312"
     CDS             complement(7875..8681)
                     /locus_tag="IVO69_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401062.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IVO69_002313"
     CDS             8819..10333
                     /locus_tag="IVO69_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7401063.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3702 a         1739 c         2055 g         2936 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACCPY010000098        10246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCPY010000098 DACCPY010000000
VERSION     DACCPY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.101"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            282..521
                     /locus_tag="IVO69_002314"
     CDS             282..521
                     /locus_tag="IVO69_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401064.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IVO69_002315"
     CDS             complement(883..1143)
                     /locus_tag="IVO69_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401065.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IVO69_002316"
     CDS             complement(1328..1825)
                     /locus_tag="IVO69_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7401066.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVO69_002317"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVO69_002317"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7401067.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVO69_002318"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVO69_002318"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7401068.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IVO69_002319"
     CDS             complement(4547..5209)
                     /locus_tag="IVO69_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7401069.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IVO69_002320"
     CDS             complement(5219..6034)
                     /locus_tag="IVO69_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7401070.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IVO69_002321"
     CDS             complement(6296..6739)
                     /locus_tag="IVO69_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7401071.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IVO69_002322"
     CDS             complement(6873..7211)
                     /locus_tag="IVO69_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7401072.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IVO69_002323"
     CDS             complement(7199..7576)
                     /locus_tag="IVO69_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7401073.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IVO69_002324"
     CDS             7800..8993
                     /locus_tag="IVO69_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401074.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IVO69_002325"
     CDS             complement(9159..9581)
                     /locus_tag="IVO69_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7401075.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IVO69_002326"
                     /pseudo
     CDS             9976..10170
                     /locus_tag="IVO69_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3111 a         2018 c         1684 g         3433 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa
    10141 aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa
    10201 gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcag
//
LOCUS       DACCPY010000099         9891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCPY010000099 DACCPY010000000
VERSION     DACCPY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.102"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..360
                     /locus_tag="IVO69_002327"
     CDS             4..360
                     /locus_tag="IVO69_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401076.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IVO69_002328"
     CDS             376..726
                     /locus_tag="IVO69_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7401077.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IVO69_002329"
     CDS             755..1534
                     /locus_tag="IVO69_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7401078.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IVO69_002330"
     CDS             1545..2222
                     /locus_tag="IVO69_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7401079.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IVO69_002331"
     CDS             2241..3347
                     /locus_tag="IVO69_002331"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7401080.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IVO69_002332"
     CDS             3337..4428
                     /locus_tag="IVO69_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7401081.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IVO69_002333"
     CDS             4441..5181
                     /locus_tag="IVO69_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7401082.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="IVO69_002334"
     CDS             5469..7409
                     /locus_tag="IVO69_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7401083.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="IVO69_002335"
     CDS             7496..9418
                     /locus_tag="IVO69_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7401084.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9698..>9891)
                     /locus_tag="IVO69_002336"
     CDS             complement(9698..>9891)
                     /locus_tag="IVO69_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7401085.1"
                     /translation="ITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTEFRK
                     VREGIKRKVLDLNQRTSIV"
BASE COUNT         3225 a         1640 c         2200 g         2826 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aactaaatac aaaaaaagag gatgtggcca aagttgtggc acatcctttt attttgagtt
     9541 tcttataatt ttttatagtt gcttacgatg tcttgactac tggtaaacgg tgttcaagag
     9601 acaatttgtc ggcaataagg cacaattttg taaaatatgg aaagctattt ttttaacatt
     9661 gtttcttagt gcttgaaaaa tcattttgaa tacttcttta tacgatcgat gtacgttgat
     9721 ttaagtctaa aacttttctt ttgattcctt ctctgacttt tctgaattcc gttaatattt
     9781 cttcttcgct cccgacagct tgtgcaggat cttttaggtc ccagtgaagg tgttcgtgat
     9841 tgtttggaac ggcagggcac ttgtctttgg catcaccaca aagtgtaatg a
//
LOCUS       DACCPY010000100         9557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCPY010000100 DACCPY010000000
VERSION     DACCPY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.104"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..344
                     /locus_tag="IVO69_002337"
     CDS             <1..344
                     /locus_tag="IVO69_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401086.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IVO69_002338"
     CDS             425..1045
                     /locus_tag="IVO69_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401087.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="IVO69_002339"
     CDS             1746..2030
                     /locus_tag="IVO69_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401088.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="IVO69_002340"
     CDS             2033..2950
                     /locus_tag="IVO69_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7401089.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="IVO69_002341"
     CDS             2943..3725
                     /locus_tag="IVO69_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7401090.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="IVO69_002342"
     CDS             3726..4406
                     /locus_tag="IVO69_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7401091.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="IVO69_002343"
     CDS             4399..5295
                     /locus_tag="IVO69_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7401092.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="IVO69_002344"
     CDS             5490..7139
                     /locus_tag="IVO69_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7401093.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="IVO69_002345"
     CDS             7733..8668
                     /locus_tag="IVO69_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7401094.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="IVO69_002346"
     CDS             8661..9431
                     /locus_tag="IVO69_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7401095.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3057 a         1584 c         2041 g         2875 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtgga
//
LOCUS       DACCPY010000101         9429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCPY010000101 DACCPY010000000
VERSION     DACCPY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.105"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..722)
                     /locus_tag="IVO69_002347"
     CDS             complement(<1..722)
                     /locus_tag="IVO69_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7401096.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="IVO69_002348"
     CDS             complement(727..1494)
                     /locus_tag="IVO69_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7401097.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="IVO69_002349"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="IVO69_002349"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7401098.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="IVO69_002350"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="IVO69_002350"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7401099.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="IVO69_002351"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="IVO69_002351"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7401100.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="IVO69_002352"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="IVO69_002352"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7401101.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="IVO69_002353"
     CDS             4894..5211
                     /locus_tag="IVO69_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7401102.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="IVO69_002354"
     CDS             5211..6917
                     /locus_tag="IVO69_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7401103.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="IVO69_002355"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="IVO69_002355"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7401104.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGFYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="IVO69_002356"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="IVO69_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..>9429
                     /locus_tag="IVO69_002357"
     CDS             8782..>9429
                     /locus_tag="IVO69_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7401105.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNCPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLD"
BASE COUNT         2973 a         1602 c         1903 g         2951 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttt tatgaccaaa tcatgcgagt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtggataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactatttta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctacccc cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaattgt
     9241 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaagat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgac
//
LOCUS       DACCPY010000102         9339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCPY010000102 DACCPY010000000
VERSION     DACCPY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.106"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IVO69_002358"
     CDS             <1..537
                     /locus_tag="IVO69_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401106.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IVO69_002359"
     CDS             564..2375
                     /locus_tag="IVO69_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7401107.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3905
                     /locus_tag="IVO69_002360"
     CDS             2436..3905
                     /locus_tag="IVO69_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP7401108.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3916..5925
                     /gene="topB"
                     /locus_tag="IVO69_002361"
     CDS             3916..5925
                     /gene="topB"
                     /locus_tag="IVO69_002361"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7401109.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6271..6867
                     /locus_tag="IVO69_002362"
     CDS             6271..6867
                     /locus_tag="IVO69_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7401110.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6880..7779
                     /locus_tag="IVO69_002363"
     CDS             6880..7779
                     /locus_tag="IVO69_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7401111.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7782..7913
                     /gene="ssb"
                     /locus_tag="IVO69_002364"
     CDS             7782..7913
                     /gene="ssb"
                     /locus_tag="IVO69_002364"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7401112.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7935..8267
                     /gene="ssb"
                     /locus_tag="IVO69_002365"
     CDS             7935..8267
                     /gene="ssb"
                     /locus_tag="IVO69_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7401113.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8289..8546
                     /locus_tag="IVO69_002366"
     CDS             8289..8546
                     /locus_tag="IVO69_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401114.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8705..8827)
                     /locus_tag="IVO69_002367"
     CDS             complement(8705..8827)
                     /locus_tag="IVO69_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7401115.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9017..9241
                     /locus_tag="IVO69_002368"
     CDS             9017..9241
                     /locus_tag="IVO69_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401116.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
BASE COUNT         3477 a         1574 c         1992 g         2296 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc
     2941 atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc
     3001 aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca
     3061 agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat
     3121 tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag
     3181 atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag
     3241 agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg
     3301 acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc
     3361 cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta
     3421 aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa
     3481 ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc
     3541 atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg
     3601 atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg
     3661 gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag
     3721 gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     3781 aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac
     3841 aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     3901 agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     3961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     4021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     4081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
     4141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
     4201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
     4261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
     4321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
     4381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
     4441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     4501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
     4561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
     4681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
     4741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
     4801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
     4921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
     4981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
     5041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
     5101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     5161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     5221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     5281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     5341 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     5401 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     5461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     5521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     5581 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     5641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     5701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     5761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     5821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     5881 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     5941 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     6001 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     6061 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     6121 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     6181 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     6241 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     6301 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     6361 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     6421 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     6481 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     6541 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     6601 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     6661 tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat ttggaatccc
     6721 tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat ggagtcggac
     6781 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga caaaacgatg
     6841 cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac gcagttaaaa
     6901 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     6961 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     7021 ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac aaccaaagat
     7081 attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac ggttctaaac
     7141 gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     7201 acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca tttggaaaac
     7261 atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc tgcaacgatc
     7321 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     7381 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     7441 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     7501 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     7561 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     7621 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     7681 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     7741 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     7801 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     7861 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     7921 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     7981 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     8041 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     8101 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     8161 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     8221 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     8281 gagtaagcat ggcaaaaaaa attagtctga cgatggatct attgataccc gtaattgcat
     8341 ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga attgcgaaag
     8401 gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa agagcaggaa
     8461 aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct gttatactta
     8521 cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt cttgcgaaat
     8581 attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt aacagcaccg
     8641 attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct gctaaagtag
     8701 acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc aaagcaatgg
     8761 taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa aggcttactc
     8821 ctttcataga ttttagcact tacattcata agcaccacca cctttccaga agtcgccacc
     8881 gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata aaaattctca
     8941 attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa tatattggtt
     9001 tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta cattacgcac
     9061 gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata aattgctgat
     9121 tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac gaaatcaact
     9181 agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgagattaa tgggcatata
     9241 aataaaagaa gagctgcagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
     9301 caagaaaaat gaaaaggcct ttttaggaag gcgcaagcg
//
LOCUS       DACCPY010000103         9081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCPY010000103 DACCPY010000000
VERSION     DACCPY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.107"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            191..1582
                     /locus_tag="IVO69_002369"
     CDS             191..1582
                     /locus_tag="IVO69_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7401117.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            1929..2576
                     /locus_tag="IVO69_002370"
     CDS             1929..2576
                     /locus_tag="IVO69_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7401118.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            2969..5614
                     /locus_tag="IVO69_002371"
     CDS             2969..5614
                     /locus_tag="IVO69_002371"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7401119.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            5924..7237
                     /locus_tag="IVO69_002372"
     CDS             5924..7237
                     /locus_tag="IVO69_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7401120.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            7227..7880
                     /locus_tag="IVO69_002373"
     CDS             7227..7880
                     /locus_tag="IVO69_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7401121.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            7935..8627
                     /locus_tag="IVO69_002374"
     CDS             7935..8627
                     /locus_tag="IVO69_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7401122.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            8777..8977
                     /locus_tag="IVO69_002375"
     CDS             8777..8977
                     /locus_tag="IVO69_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7401123.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2928 a         1543 c         1993 g         2617 t
ORIGIN      
        1 agattaaagc tatcacaggt atgttttttt attactaatt gtaagcaaaa agaatgtcat
       61 ttcgataaaa atcgtctaaa atataaaagt cactgaaaaa aattttttca ggaggatggt
      121 tgaagaatca ttctcgggaa cctttacttc cttcgatgga cttgtcatta ataatatgag
      181 gaggagaaaa atgacaacac tcatcacagt tttagtggtt ctagtttttg cggcattatt
      241 atatgtgttc tatcgtatgc aaaagcgcca tacaaaattt tcaacacgcg tctttacagc
      301 attaggagtt ggtatcgtct ttggcggtct gcttcaattg atctttggtg tagacgacaa
      361 agtcgttact caatcaatgg aatggatcgg gattgtaggt caaggatatg tatcattctt
      421 gcaaatgcta gttatgcctt tagtgtttat ttcgatcatt ggtgcattca caaaaatgaa
      481 agtttctgaa aagctaggga aaattagtgc gactgtattg acttctttac ttggaacaac
      541 agctattgca gcattgatcg gaattggttc aacaatgctg ttcggactcc aaggagctaa
      601 attcacacaa ggagctgcag aaacagcacg aatctctgaa ctggcaacac gtcaggaaac
      661 agttgaaaac ttatcgattc cacagcagat tttatccttt atccctaaaa acgtttttgc
      721 tgatttagca ggtagtcgtg caacaagtac gattggagtc gttattttcg cggcatttgt
      781 cggaatcgct tatctaggcg tgaaaagaaa agcgccaaaa gaagcagcat tcttttcaga
      841 tttagtcgac agtctgtata aaatcattat gcggatcgtg acgcttgttt tgcgtttgac
      901 accttatgga gtatttgctt tgatgacaaa cgttttggca acaagtgatt ttgcagctat
      961 cgtcaattta ggtaaatttg tcgttgcttc ttacgtagca ttgcttgctg ttttgattgt
     1021 ccactcactg attttgattg gtgcaaaagt caatccaatc aactatttca aaaaagcctt
     1081 tccggtattg agctttgcgt ttacttcacg ttcaagtgct ggagctttgc cattgaatat
     1141 cgagactcag acaaaagctt taggagtaga tgatgcaaca gctaatttct cagcaagttt
     1201 tggtttgtct attggacaaa acggctgtgc tggcgtttat ccagctatgt tggcaacgat
     1261 cgtcgcacca gcagctggag tgaatgtctt tagtttggaa tttattttga tgcttgtggc
     1321 aattgtaaca atcagttctt ttggtgtagc tggagtcggt gggggcgcaa catttgcttc
     1381 actgatcgtt ttaggggcaa tgaacttgcc agtagcaatc gttgggttag ttatctctgt
     1441 tgaaccatta atcgatatgg cacgtacagc agtaaacgtc agcggtagca tggtagctgg
     1501 tatcgtgaca agtgcacgca tcaaagattt agatcgcgat gtcatcaatg atgattctaa
     1561 agtgatcgat gtgaatgcat agtcaaaaag aggttgtgat agaagtggtc agctttaaga
     1621 aataagaaga agtttcctaa aaatagtttc tcaaattttt gtgaaatttc ggcttatttc
     1681 cgagaagcta cttctgaaac accgtgaata agaggttgtg aaaaagctgc cctgcatcaa
     1741 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
     1801 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
     1861 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
     1921 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
     1981 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
     2041 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
     2101 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
     2161 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
     2221 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
     2281 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
     2341 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
     2401 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
     2461 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
     2521 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
     2581 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
     2641 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
     2701 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
     2761 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     2821 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     2881 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     2941 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     3001 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     3061 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     3121 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     3181 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     3241 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     3301 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
     3361 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     3421 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     3481 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     3541 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     3601 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     3661 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     3721 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     3781 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     3841 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     3901 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     3961 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     4021 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     4081 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     4141 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     4201 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     4261 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     4321 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     4381 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     4441 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     4501 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     4561 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     4621 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     4681 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     4741 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     4801 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
     4861 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     4921 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     4981 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     5041 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     5101 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     5161 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     5221 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     5281 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     5341 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     5401 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     5461 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     5521 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     5581 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     5641 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     5701 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     5761 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     5821 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     5881 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     5941 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     6001 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     6061 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     6121 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     6181 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     6241 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     6301 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     6361 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     6421 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     6481 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     6541 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     6601 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     6661 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     6721 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     6781 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     6841 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     6901 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     6961 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     7021 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     7081 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     7141 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     7201 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     7261 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     7321 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     7381 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     7441 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     7501 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     7561 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     7621 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     7681 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     7741 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     7801 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     7861 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     7921 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     7981 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     8041 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     8101 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     8161 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     8221 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     8281 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     8341 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     8401 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     8461 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
     8521 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
     8581 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
     8641 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
     8701 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
     8761 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
     8821 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
     8881 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
     8941 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
     9001 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
     9061 caatttctct tgtggcaggt a
//
LOCUS       DACCPY010000104         8894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCPY010000104 DACCPY010000000
VERSION     DACCPY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.108"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(221..1177)
                     /locus_tag="IVO69_002376"
     CDS             complement(221..1177)
                     /locus_tag="IVO69_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7401124.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IVO69_002377"
     CDS             1281..1883
                     /locus_tag="IVO69_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7401125.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IVO69_002378"
     CDS             complement(1971..3470)
                     /locus_tag="IVO69_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7401126.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IVO69_002379"
     CDS             complement(3486..4010)
                     /locus_tag="IVO69_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401127.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            complement(4096..4281)
                     /locus_tag="IVO69_002380"
     CDS             complement(4096..4281)
                     /locus_tag="IVO69_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401128.1"
                     /translation="MAETIKETNVEKQTTRMIVFWSFNGFFLKKGNIGYLERIYCENF
                     NMKTKFNKLSTINTTFL"
     gene            4307..5056
                     /locus_tag="IVO69_002381"
     CDS             4307..5056
                     /locus_tag="IVO69_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7401129.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5047..5832
                     /locus_tag="IVO69_002382"
     CDS             5047..5832
                     /locus_tag="IVO69_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7401130.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5801..6517
                     /locus_tag="IVO69_002383"
     CDS             5801..6517
                     /locus_tag="IVO69_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7401131.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6619..7638)
                     /locus_tag="IVO69_002384"
     CDS             complement(6619..7638)
                     /locus_tag="IVO69_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7401132.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7657..8715)
                     /locus_tag="IVO69_002385"
     CDS             complement(7657..8715)
                     /locus_tag="IVO69_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7401133.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2683 a         1796 c         1590 g         2825 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaattttgt
     4141 tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc ctttttttaa
     4201 gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct ccacattcgt
     4261 ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga accgatctag
     4321 tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac tcatccaaaa
     4381 aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg aaatcatgat
     4441 tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat attctcacgc
     4501 agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg
     4561 aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt
     4621 ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa caaacaacac
     4681 gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga ttattggact
     4741 tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg
     4801 acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt
     4861 agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca atacacctgc
     4921 aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa aaaaatcagt
     4981 gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac cgttcggact
     5041 caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct ttttagcaat
     5101 gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat gaaattaatg
     5161 gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac ccatgaattc
     5221 atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga agaagtcacc
     5281 cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg
     5341 atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc caaagaacaa
     5401 tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat ggatgactcc
     5461 atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga agccgtgcta
     5521 caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac agaagctgaa
     5581 gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa
     5641 atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt gaatctcatc
     5701 cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac aaatggcggt
     5761 acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa atgtcctgat
     5821 aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa
     5881 tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa
     5941 aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta tccatttttt
     6001 atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg ctgcagatga
     6061 tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga tcaaaccgtt
     6121 cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc agacaatcca
     6181 aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca ccactcccat
     6241 tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa
     6301 aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac tggctcaaaa
     6361 aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag aacaagatat
     6421 attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt atgaattgac
     6481 aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat cccatacaaa
     6541 atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa cattttattg
     6601 acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata cctcattttt
     6661 cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga
     6721 taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag ggtacatttc
     6781 tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa
     6841 aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa
     6901 agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa tcaagatgct
     6961 accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt cacctgggat
     7021 atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa gcgagtggaa
     7081 cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca ctttggctcc
     7141 tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta tgatccctac
     7201 tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat ctttgaaaga
     7261 tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag tcaaagataa
     7321 ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac caaaatcagc
     7381 tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat aacgaatccc
     7441 atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc tcaacaatac
     7501 agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt cataaagact
     7561 gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg gacgaacacc
     7621 gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac ctatgccatg
     7681 aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa tagttgacag
     7741 taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc
     7801 cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt caaaattgac
     7861 tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg caggtacaaa
     7921 taccactttg actagactgt aataccatga agttttttca gatgcagctt ttttgaagct
     7981 tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta aaccagctaa
     8041 ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa acgctacttg
     8101 gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt ggaacagcca
     8161 aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg ctttgatatt
     8221 tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca aaaagattcg
     8281 gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa tcgcactgac
     8341 gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca acgttgtttg
     8401 cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa taatataaat
     8461 aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat tgatatcttg
     8521 catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt tcgatagaat
     8581 ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta ataatataat
     8641 aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg atcgaaaaat
     8701 gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat aaaaaaagct
     8761 tttattttgc taatacgtat taacattttt aattttatct ttacattgtt acttattgaa
     8821 caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttttact agaacacaaa
     8881 aaaaacctta aaac
//
LOCUS       DACCPY010000105         8622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCPY010000105 DACCPY010000000
VERSION     DACCPY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.109"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..1960
                     /locus_tag="IVO69_002386"
                     /pseudo
     CDS             4..1960
                     /locus_tag="IVO69_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
     gene            1950..3545
                     /locus_tag="IVO69_002387"
     CDS             1950..3545
                     /locus_tag="IVO69_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7401134.1"
                     /translation="MKNKWCKSAVIYQIYPRSFQDTNGDGIGDIPGILSRLDYLENLG
                     INAIWLSPVYESPNDDNGYDISDYRKIGAEYGTMADMDLLIKEARQRGIRIIMDLVIN
                     YTSDRHPWFQLAKKSVTSPYHDYYVWRKEPNGLESNFGGSAWTYVPEVNQYYLHLHSK
                     KQPDLNWQNPKMRQAIYDMMNFWLEKGIGGFRLDVIDLIGKQPEQSITKNGPLLHDLL
                     QEMYQKTFGHYDVVTVGETWGATPKIAQLYSKEERKELSMVFQFEHINLDKQNGKRKW
                     DLKDLDPQELHRTFSKWQIELGGCGWNSLFWNNHDLPRIISRWGDDQEYRTISGKMLA
                     IYLHFMQGTPYIYQGEEIGMINYPVKSIDEVDDIESQRMYAERIKKGYSKEELIQSIN
                     SKGRDNARHPMQWNNHKNGGFTEGTPWLPVGNTEEINVEAALAAQDSLFYTYQKLIEL
                     RKELPVIVYGKYQVIETEEPNVLAYLRSLKKQKILVVVNFSDQKTTFPIGKYRVKQVL
                     IQNYKKKITNNLLPYEAFSALIY"
     gene            complement(3740..4075)
                     /locus_tag="IVO69_002388"
     CDS             complement(3740..4075)
                     /locus_tag="IVO69_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7401135.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            4361..6241
                     /locus_tag="IVO69_002389"
     CDS             4361..6241
                     /locus_tag="IVO69_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7401136.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPLTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDDVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVVIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            6320..6547
                     /locus_tag="IVO69_002390"
     CDS             6320..6547
                     /locus_tag="IVO69_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7401137.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDKVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            6552..7094
                     /locus_tag="IVO69_002391"
     CDS             6552..7094
                     /locus_tag="IVO69_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7401138.1"
                     /translation="MNIVEEKFAQEIKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLLASTILENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            7339..8058
                     /locus_tag="IVO69_002392"
     CDS             7339..8058
                     /locus_tag="IVO69_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7401139.1"
                     /translation="MKKRILLIMGLAFLLAGCTNGNEPRKESTKETAKTTIATTTTTS
                     TESKSTATSTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KAAQLEAFMTTWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFDQIVYGQVNQ"
     gene            8388..>8622
                     /locus_tag="IVO69_002393"
     CDS             8388..>8622
                     /locus_tag="IVO69_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7401140.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPL
                     LVFFLTMASIAKHKIGNKTFVKPILVLYLVGTLL"
BASE COUNT         2824 a         1413 c         1853 g         2532 t
ORIGIN      
        1 gaaatggatt acaaaaaagt agctgaagaa atcagcaaag cgcttggaga agacaatgta
       61 caagcagctg cgcattgtgc aacacgttta cggcttgtct taaaagattc agacaaagta
      121 gaccagaagg ctctggatga taatgaaagt gtgaaaggaa cttttgaagc aaatgatcaa
      181 tttcagatca ttatcggagc aggtgatgtg aataacgtat atgatgaatt cgttaagatc
      241 accggcgtaa aggaagcttc aaccgacgat ttgaaagcgg tagcatcgaa taagaagaat
      301 aatactgtca tggcctttat caaggtgttg tcagatatct tcgttccgat tgtccctgca
      361 ttggttgcgg gtggactgtt gatggctctg aataatgttt tgacggcaca ggatttgttt
      421 ggtcctcagt cagtggttga gttgtatcca tcagtgaaag attttgctgg catcattaat
      481 ttacttgctt cagcgccgtt tgcttttttg ccgattttgg taggtttttc agcaactcga
      541 cgttttggag gcaatccgta tcttggagca gcaatgggga tggcgatggt catgccggat
      601 ttagtgaatg gatacagtgt gacgaatgct attgcagaag gatcgatgcc ttattggaat
      661 atttttggaa tgaatgtggc acaagccggt tatcaaggat ctgttcttcc agtccttgca
      721 gtttcttgga ttttagctac tttagaaaaa ttcttccata aacggttggc taatgctttc
      781 gactacacat ttactccaat gctagctatt attattactg gttttttaac ttttacagta
      841 gtagggccgg tgatgcgtac tgtcggagac ggcttagctg atggattaag ctggttatac
      901 agtactactg gagctttcgg cttaggtgtc tttggtttct tctattcgcc gatagtcatt
      961 accggattac atcaaagctt tccagcaatc gagactactt tactagcaga tgttgccaga
     1021 acgggtggct cttttatctt tccagttgcc tcaatggcaa atgtagcgca aggagcggct
     1081 gctttggcga ttatgctgct gacaaaaaat aaaaagcaaa agagtctagc taccactgca
     1141 ggtgtttccg ctatgttggg gattactgag ccagcgatat tcggtgtgaa tttgaaactg
     1201 aaatttccgt ttatttgtgg aatgattgct gcaggaattg cttcattctt tatcggcttg
     1261 ttccacgtat tagcagttgc gatgggaccg gcaagcgtca ttggctttat ttccattgca
     1321 cctcgttcga ttccttcatt catgatcgga attgttatca gtatagtgat cggttttttt
     1381 aagcacttac ttctatggga aaaaacatat ggaactgcta gcagatggtg aatcaaaagg
     1441 aacagaagca gctgagtcaa tcagttcagc tcctaaaacg actaggaaac cgcaagatga
     1501 aattttagct gcatcagtca gcgggaagat tctcccttta acgaaggtaa aagatccagt
     1561 tttttccact gaaatgatgg gaagaggtat agcagtcaaa ccagatgacg gggtagtcca
     1621 tgctccagca gatggtatat tgacagttgt ccacgaatct aaacatgcat atggcttgaa
     1681 aacagagaaa ggagcggaag tactgatcca tatcggtatc gatacagtga atctaaaagg
     1741 ggccggattc acgacacagc gacaatatgg tgaattagtg aaaaaagggg atattctcgg
     1801 tacatttgat cgtaaattgc ttaaagaaga agggtacgac gatacaatta tggaaattgt
     1861 cactaattcc tctgcatatg ctaatgtaaa aattcttgaa aaaaacacgg tcaaaagtgg
     1921 tgaagatatt ctgatagtga gtgaaacaga tgaaaaataa atggtgtaaa agtgcagtaa
     1981 tctatcaaat ctatcctcga agttttcaag atacaaatgg agatggcatt ggagatattc
     2041 ctggtattct ttcaagatta gattatctag aaaacctggg aatcaatgcg atatggttaa
     2101 gcccggttta tgaatcacct aatgatgata atggctatga tatcagtgac tacagaaaaa
     2161 taggcgcaga atatggaacg atggcagata tggatttact gataaaagaa gcacgacaaa
     2221 ggggaataag aatcatcatg gatcttgtta tcaattatac gtcagacaga catccatggt
     2281 ttcaattggc aaaaaaaagt gttaccagtc cctatcacga ctattatgtt tggcgtaaag
     2341 aaccgaatgg attagagtcg aacttcggcg gttcagcatg gacctatgtg cccgaagtga
     2401 atcaatatta tctgcacttg cattcgaaaa agcagccgga tttgaattgg caaaatccaa
     2461 agatgagaca agcaatctat gacatgatga atttttggct tgagaaaggg attggcggat
     2521 ttcgattaga tgtcattgat ctgattggaa aacagccgga acaatctatt actaaaaatg
     2581 gtcctctttt acatgattta ttgcaagaaa tgtaccaaaa aacttttggt cattatgatg
     2641 tagtgacagt gggagagact tggggagcaa cgcccaagat tgcgcagctg tattcaaaag
     2701 aagaacgcaa agaattatca atggtctttc aattcgagca tattaatcta gacaaacaga
     2761 atggcaagcg aaaatgggat ctaaaggatt tagatccaca ggaattacat cggacttttt
     2821 ctaaatggca gattgaatta ggtggttgtg gttggaattc tttattttgg aataatcatg
     2881 atctcccacg aatcatttct cgctgggggg acgatcaaga atatcgaaca atcagtggaa
     2941 aaatgctagc gatatacctc cattttatgc aaggtactcc ttatatctat caaggagaag
     3001 agatcggcat gatcaattat ccagtaaaga gtattgatga ggtagatgat attgaaagcc
     3061 aaagaatgta tgcagaacgt ataaaaaaag gctattcgaa ggaagagttg atccaatcaa
     3121 tcaactcgaa ggggcgagat aatgctcgcc atccgatgca atggaacaat cacaaaaatg
     3181 gtggctttac ggaaggcact ccttggttgc cagtggggaa tacagaagag atcaatgtag
     3241 aagcagcatt agctgctcaa gattccttgt tttatacgta tcaaaaactg atcgaattgc
     3301 gcaaagaatt acctgtgatt gtctatggga aatatcaagt cattgagaca gaggaaccga
     3361 atgttttagc atatctacgc agtttaaaaa aacaaaaaat actagttgta gttaattttt
     3421 ctgatcaaaa gactactttt ccaatcggta agtatcgtgt caaacaagtc ttgatccaaa
     3481 attacaaaaa gaaaattact aacaatttac tcccatatga agcttttagc gcgctgatct
     3541 attaagtggt tttttctata gatgaatctt tgttatgagg tagatagttc aatgaaacag
     3601 ttattcgatt tgtttataga aaacaattta atcagcaaaa agggggacaa acttttaatt
     3661 gtctcccttt aattgttagg atggaatatt atcactctta aacaataagt taaaaaaata
     3721 ctcgtttaca taaaaaagat tatttagatg cgataatatc atagataaag aaacattcat
     3781 cataagaaac agtgatccca aattctgttt caatattttt gaaggccttt tttacatgaa
     3841 aaattgtttc atgagaaggt cttgtgtcat cttctgatat aattttaagt tcatgaccgt
     3901 ttatcaatgt acgctcaatt agacacgctg tatgaatcaa aagttttaaa agaatggaat
     3961 gagtgaattt tttgtggaaa gtacattcca attcttggat aaaatccatt aagctttttt
     4021 ggatcttatt cccagctaat aagtagagat atttgtccac tgcttcaatt cccatatcaa
     4081 aaaacgatat ttcttgtgaa tgagtggctg ctgccctttt ttgattgttg ttcttcagtc
     4141 gtaagatgac aaagcatctt atctttgaat aaaaaaatag aactacctaa ggaagcgttt
     4201 cggcgcttcc tttttctata tgatcgttta atgactgtag gaattcagta taccaaaaga
     4261 aaaccacctt tttgactaaa cttgatagtg gtcaattttt tatgatggat aagtatctat
     4321 acttagtctc gtaagaatga aaaggaaaag agggagaaag atgaaatttc aaaagtggat
     4381 ccaagagcat cgaaatcata tcaccgcctt atcagctgtg ttgattattt ttggttttat
     4441 cggaaaattt actggatttg tcttgttcta taacattgct ctagccatag cttctattat
     4501 tgcggtcata cctattatga tccatgcgta tcaggcagta aaagtcaaag ttatctcaat
     4561 tgatttactc gttactattg ctgtgcttgg tgcctttatg atcggagaat acaaggagtc
     4621 agctatcgtt acattcttgt ttttgtttgg tagttatctg gaacaaaaaa cattgaccaa
     4681 aacgcgggaa tcaatcaagg ctttgaccga gatggcccca ttgactgcca gcgttgtcca
     4741 agaagatgga acgactgaag aagtggaaat cgatgacgtg gaagaaggtg atatcgtctt
     4801 agtaaaaact ggtggagcta ttcctgtgga cggacgagtc attgacggaa aagggtatgc
     4861 ggatgaagcc gcagtgacgg gtgaatcacg acaagtcgtc aaaatcagtg gagatgacgt
     4921 attttctgga acgattttag cagatggcta cgtgacgatc gaagcaacta aggtcggaga
     4981 tgatacgacc tttgccaaaa ttatcgaatt agtcgaagaa gcacaagata ccaaatcaca
     5041 tgcggaaaaa ttcattgatc gtttttctca gtattatact ccaaccgttt tacttattgc
     5101 gttgcttgtt ggtctgatca gtcgtgattt tcgtttagcc atcactgttt tggttcttgg
     5161 ttgtcctggc gctttagtca ttggtgcgcc tgtttcgaat gtagcaggaa tcggtaatgg
     5221 agcgaaacgg ggcatcttag tcaaaggcgg tgaagcaatg gatacttttt ccaaaatcga
     5281 tacttttgtc tttgataaaa ccggtacatt gaccaaagga actactgctg tatccactat
     5341 caagaactat acagaggatg aaggggacag cttagcttta gtggctcgat tagaaacgtt
     5401 atccgatcat cctttggggc gcgccgtgat ccattatgct caggagaaag acattgattt
     5461 tcaacaatta gcagtcaaag ataatcagac gatcaaggga caaggaatca cagctgagat
     5521 tgatggtcat acagtttgtg cgggcaacac caaattgatt gccgctcaca aactttctct
     5581 cacaccaaag caagtacaag atttacatca attacaacaa acaggcagct cggtcattat
     5641 tgtagtaatc gacaaaaaaa tcactcaaat tattggtgtg tcagatgtga ttcgaccaga
     5701 agtcgctgaa caattagcta tgttgaaaca agcaggagca aaacatttag tgatgctgac
     5761 aggtgataat cagatgactg ctgattatgt agctgatatg ttaggcattg atgaagtcca
     5821 tgctgaatta ttaccagatg aaaaggttca atttgtgaaa aaataccaag aacaaggatt
     5881 gcgcgttgct ttcattggag atggaatcaa tgattctccc tctttggctg cggctgatat
     5941 cgggattgct atgggatcag gaacggatgt tgctatcgaa acgagtgacg ttgtcttgat
     6001 gcaatctagt tttggatcgt tggttcatgc ctaccgtttg gctaagaaaa ctgttttgaa
     6061 tacccgtgaa aatgtagtga ttgccattgg tgtcgtctta tttttactca tcggcttatt
     6121 tgccgggttt atttacatgg ctagtgggat gttcgttcac gaagctagta tcttgatcgt
     6181 tatctttaac gcgatgcgtt tattgtattt tggacacgaa agcaaagagg aaaaatcgta
     6241 aaacttgata agaatcaatt tttttatgga agctgtaatc tagaataaga ctatcaagaa
     6301 aaagggagga agaactataa tgaaaaaaat gattatgcaa ttagatacac tagcttgtcc
     6361 aagctgtatg caaaaaatcg aacatgcggt ttcgcaacag cctggggtaa gcaatacgaa
     6421 agttttgttt aatgcgagca aaatcaaagc aaactttgat ccagataaag ttcaccctga
     6481 acaattagct gcagttgtgg aaaaattagg ttatgaagtc caatcaacaa aagtaaaaga
     6541 ggcataaatc aatgaatata gtggaagaaa aatttgctca agaaataaaa caagcagata
     6601 tcgaccatca taaaccaact gctggcgcca tggtggggca tgtattagca aatttcaaat
     6661 tagaagaaat gaaattgatc caagcacaat tgtatgtttt gggtacatct tcgcaagaat
     6721 taagtaccct tttttcagaa tatgctgatc aagaagcgag atggttcaat aaaatctctc
     6781 atgagttagt gattgagaat gaaatcgttc cgtctacttt agaagaagtc atgcgttatg
     6841 gaaaaattta cgaacatggt caaaacaaat atttactagc ctcaacaata ttggaaaact
     6901 ttgcaaaaga ttttgatttc caaaatgtct ttatcacacg ggcaatcaag ttagctgaaa
     6961 aagaagaaaa atatggcttg caacaattac tcgttgaact tttaagtttc aataaagaaa
     7021 tcatctacca agtacaaaga ctccaaggaa aaactgtccg acaagatttg gatgaggaag
     7081 acgatgatga ctaaaagtct atgtttcttc aaacacaaat ataaagcgtg agaaaagcag
     7141 gaaaaaaaca cgaagttttt gtcttaaaaa taaaataaaa agaagcaccc gctgatgttg
     7201 tctataagac atcagcgggt gcttcttagt ttttaaatgg tttaaaagat ggatgagaaa
     7261 atacaaaaaa tcaataaaat cacattgctt atttgataga ataaagaagt gggggagtaa
     7321 tcgaaagggg ttttttaatt gaaaaaaagg attttattaa taatgggtct agcttttttg
     7381 ttagctggtt gtaccaacgg gaatgaacca agaaaagaat caacaaaaga aacagcgaaa
     7441 acaacaattg caactactac aacaactagc acagagagca agtcaactgc aacaagtaca
     7501 gaaacagcag atacctctac ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca
     7561 tctacttcaa gttcttctgt agaggcttca tcttcctctt cagaaagcag cgaacaagtc
     7621 gaacaaacac tgtggaatgc agacaaagcc gcacaattag aggcatttat gaccacatgg
     7681 ggccaaacaa tgggacaaca atacaaaagt tatacgaatc aaatgagtgt ggacttatat
     7741 ggtcttaaag tccctcaagc tattttgaat ggggagtgga agatggctat tggtggagtg
     7801 cctgtttctg cagaatggtc agaaagcggg acaggtcaag cagattatca aatcacagcc
     7861 gtctattccg atgcagagac agaaccatat ctgaaaaagc acgtctatct attcggtttt
     7921 cagcaaaatc agccaaaagt attggtgaca caacaaaatc aggggaatcc cgataattat
     7981 ctttacttca acgagacagc gaataatgaa ttgaaaaatg gctttgatca aatcgtttat
     8041 gggcaagtaa accagtgaga gtgagagaaa gaagagactc agtggaaaat attaattgat
     8101 tgttatgtca aagaattaag atgaaggatg aaatttctag tttcgtcctt catcttagtt
     8161 acgttttact tggataactt taataataac gataaatagt tttctctcga cttctttaga
     8221 gattgaataa accctccaat actaacattg aatgttgcaa gagcatgaat tgccaattgt
     8281 attattagaa tttttagaaa attgcacctt tagtttcttt agataaaatg atacaatgat
     8341 tggtaaaatt gtgaaagggt ggtcatttgt tagagaagtt tttggatatg tcgttagttc
     8401 aacgtattgt tgttggtatt ttagtcggaa tattgtttgg tatctttttc cccacgtgga
     8461 catttatttc aatattgggt acgttgtttg tcagttgctt gaaagcaatt gcgcctcttc
     8521 tagttttttt cttgacgatg gcttcaattg ccaaacataa aataggaaat aagacatttg
     8581 tgaagcctat tttagtctta tatttagtag gaacattgct tt
//
LOCUS       DACCPY010000106         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCPY010000106 DACCPY010000000
VERSION     DACCPY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.110"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            264..1457
                     /locus_tag="IVO69_002394"
     CDS             264..1457
                     /locus_tag="IVO69_002394"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7401141.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IVO69_002395"
     CDS             1587..3059
                     /locus_tag="IVO69_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401142.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IVO69_002396"
     CDS             3142..3930
                     /locus_tag="IVO69_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7401143.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IVO69_002397"
     CDS             complement(3997..4665)
                     /locus_tag="IVO69_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7401144.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IVO69_002398"
     CDS             4780..6492
                     /locus_tag="IVO69_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7401145.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IVO69_002399"
     CDS             6495..8267
                     /locus_tag="IVO69_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7401146.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1828 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACCPY010000107         8296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCPY010000107 DACCPY010000000
VERSION     DACCPY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.111"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IVO69_002400"
     CDS             complement(51..1517)
                     /locus_tag="IVO69_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7401147.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IVO69_002401"
     CDS             1904..2500
                     /locus_tag="IVO69_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7401148.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IVO69_002402"
     CDS             complement(2558..3040)
                     /locus_tag="IVO69_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401149.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IVO69_002403"
     CDS             complement(3146..3343)
                     /locus_tag="IVO69_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401150.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IVO69_002404"
     CDS             3638..4396
                     /locus_tag="IVO69_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7401151.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IVO69_002405"
     CDS             4456..4884
                     /locus_tag="IVO69_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401152.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IVO69_002406"
     tRNA            4930..5002
                     /locus_tag="IVO69_002406"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IVO69_002407"
     CDS             complement(5152..5523)
                     /locus_tag="IVO69_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7401153.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IVO69_002408"
     CDS             5738..5923
                     /locus_tag="IVO69_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401154.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IVO69_002409"
     CDS             complement(6006..6194)
                     /locus_tag="IVO69_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401155.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IVO69_002410"
     CDS             complement(6218..6481)
                     /locus_tag="IVO69_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401156.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IVO69_002411"
     CDS             6639..6896
                     /locus_tag="IVO69_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7401157.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IVO69_002412"
     CDS             complement(6967..7158)
                     /locus_tag="IVO69_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401158.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IVO69_002413"
     CDS             complement(7299..7619)
                     /locus_tag="IVO69_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401159.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IVO69_002414"
     CDS             complement(7642..8076)
                     /locus_tag="IVO69_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7401160.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1637 c         1314 g         2712 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ccgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DACCPY010000108         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCPY010000108 DACCPY010000000
VERSION     DACCPY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.112"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IVO69_002415"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IVO69_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7401161.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IVO69_002416"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IVO69_002416"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7401162.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IVO69_002417"
     CDS             2591..3376
                     /locus_tag="IVO69_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7401163.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IVO69_002418"
     CDS             3389..4240
                     /locus_tag="IVO69_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7401164.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IVO69_002419"
     CDS             complement(4364..4816)
                     /locus_tag="IVO69_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401165.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IVO69_002420"
     CDS             complement(5281..5649)
                     /locus_tag="IVO69_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7401166.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IVO69_002421"
     CDS             5799..6281
                     /locus_tag="IVO69_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7401167.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IVO69_002422"
     CDS             6318..7328
                     /locus_tag="IVO69_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7401168.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IVO69_002423"
     CDS             complement(7443..8156)
                     /locus_tag="IVO69_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401169.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACCPY010000109         7979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCPY010000109 DACCPY010000000
VERSION     DACCPY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.113"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVO69_002424"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVO69_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7401170.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IVO69_002425"
     CDS             1228..2331
                     /locus_tag="IVO69_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7401171.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVO69_002426"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVO69_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7401172.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IVO69_002427"
     CDS             complement(3821..6268)
                     /locus_tag="IVO69_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7401173.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IVO69_002428"
     CDS             complement(6457..7893)
                     /locus_tag="IVO69_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7401174.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2215 a         1736 c         1358 g         2670 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaa
//
LOCUS       DACCPY010000110         7628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCPY010000110 DACCPY010000000
VERSION     DACCPY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.114"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IVO69_002429"
     CDS             complement(14..706)
                     /locus_tag="IVO69_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7401175.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVO69_002430"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVO69_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVO69_002431"
     CDS             complement(1143..1712)
                     /locus_tag="IVO69_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7401176.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVO69_002432"
     CDS             complement(1786..2109)
                     /locus_tag="IVO69_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401177.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVO69_002433"
     CDS             complement(2263..3075)
                     /locus_tag="IVO69_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401178.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVO69_002434"
     CDS             3429..4277
                     /locus_tag="IVO69_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7401179.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVO69_002435"
     CDS             4422..5363
                     /locus_tag="IVO69_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7401180.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVO69_002436"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVO69_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2473 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttt
//
LOCUS       DACCPY010000111         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCPY010000111 DACCPY010000000
VERSION     DACCPY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.115"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(135..425)
                     /locus_tag="IVO69_002437"
     CDS             complement(135..425)
                     /locus_tag="IVO69_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401181.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IVO69_002438"
     CDS             complement(543..803)
                     /locus_tag="IVO69_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401182.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(945..1352)
                     /locus_tag="IVO69_002439"
     CDS             complement(945..1352)
                     /locus_tag="IVO69_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7401183.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKLS"
     gene            complement(1808..2293)
                     /locus_tag="IVO69_002440"
     CDS             complement(1808..2293)
                     /locus_tag="IVO69_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401184.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2647)
                     /locus_tag="IVO69_002441"
     CDS             complement(2432..2647)
                     /locus_tag="IVO69_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7401185.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IVO69_002442"
     CDS             2871..3671
                     /locus_tag="IVO69_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7401186.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IVO69_002443"
     CDS             3690..5558
                     /locus_tag="IVO69_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7401187.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IVO69_002444"
     CDS             5551..6042
                     /locus_tag="IVO69_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7401188.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IVO69_002445"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IVO69_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7401189.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IVO69_002446"
     CDS             6602..>7596
                     /locus_tag="IVO69_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7401190.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACCPY010000112         7320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCPY010000112 DACCPY010000000
VERSION     DACCPY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.116"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            212..1045
                     /locus_tag="IVO69_002447"
     CDS             212..1045
                     /locus_tag="IVO69_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7401191.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            1079..1918
                     /locus_tag="IVO69_002448"
     CDS             1079..1918
                     /locus_tag="IVO69_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7401192.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            1944..2504
                     /locus_tag="IVO69_002449"
     CDS             1944..2504
                     /locus_tag="IVO69_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7401193.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            2703..4424
                     /locus_tag="IVO69_002450"
     CDS             2703..4424
                     /locus_tag="IVO69_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7401194.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            4589..5731
                     /locus_tag="IVO69_002451"
     CDS             4589..5731
                     /locus_tag="IVO69_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7401195.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            5747..6958
                     /gene="thiI"
                     /locus_tag="IVO69_002452"
     CDS             5747..6958
                     /gene="thiI"
                     /locus_tag="IVO69_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7401196.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2576 a         1222 c         1540 g         1982 t
ORIGIN      
        1 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
       61 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
      121 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
      181 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
      241 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
      301 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
      361 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
      421 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
      481 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
      541 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
      601 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
      661 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
      721 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
      781 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
      841 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
      901 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
      961 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     1021 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     1081 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     1141 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     1201 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     1261 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     1321 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     1381 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     1441 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     1501 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     1561 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     1621 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     1681 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     1741 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     1801 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     1861 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     1921 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     1981 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     2041 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     2101 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     2161 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     2221 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     2281 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     2341 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     2401 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     2461 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     2521 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     2581 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     2641 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     2701 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     2761 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     2821 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     2881 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     2941 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     3001 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     3061 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     3121 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     3181 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     3241 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     3301 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     3361 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     3421 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     3481 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     3541 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     3601 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     3661 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     3721 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     3781 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     3841 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     3901 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     3961 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     4021 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     4081 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     4141 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     4201 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     4261 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     4321 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     4381 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     4441 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     4501 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     4561 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     4621 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     4681 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     4741 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     4801 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     4861 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     4921 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     4981 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     5041 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     5101 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
     5161 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
     5221 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
     5281 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
     5341 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
     5401 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
     5461 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
     5521 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
     5581 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
     5641 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
     5701 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
     5761 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
     5821 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
     5881 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
     5941 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
     6001 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
     6061 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
     6121 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
     6181 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
     6241 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
     6301 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
     6361 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
     6421 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
     6481 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
     6541 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
     6601 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
     6661 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
     6721 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
     6781 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
     6841 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
     6901 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
     6961 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
     7021 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
     7081 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
     7141 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
     7201 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
     7261 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
//
LOCUS       DACCPY010000113         7165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCPY010000113 DACCPY010000000
VERSION     DACCPY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.117"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..813
                     /locus_tag="IVO69_002453"
     CDS             <1..813
                     /locus_tag="IVO69_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7401197.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVO69_002454"
     CDS             807..1229
                     /locus_tag="IVO69_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401198.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1533..1913
                     /locus_tag="IVO69_002455"
     CDS             1533..1913
                     /locus_tag="IVO69_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7401199.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1895..2809
                     /locus_tag="IVO69_002456"
     CDS             1895..2809
                     /locus_tag="IVO69_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7401200.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3049..3738
                     /locus_tag="IVO69_002457"
     CDS             3049..3738
                     /locus_tag="IVO69_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401201.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYKEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3859..3945)
                     /locus_tag="IVO69_002458"
     CDS             complement(3859..3945)
                     /locus_tag="IVO69_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7401202.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4590..5327
                     /locus_tag="IVO69_002459"
     CDS             4590..5327
                     /locus_tag="IVO69_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7401203.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5356..5877
                     /locus_tag="IVO69_002460"
     CDS             5356..5877
                     /locus_tag="IVO69_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7401204.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENN
                     NAITTEKKWWKFW"
     gene            5961..6265
                     /locus_tag="IVO69_002461"
                     /pseudo
     CDS             5961..6265
                     /locus_tag="IVO69_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6824..>7165
                     /locus_tag="IVO69_002462"
     CDS             6824..>7165
                     /locus_tag="IVO69_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7401205.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2722 a         1173 c         1229 g         2041 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgctagcca aatacgtagg tatttggcta gcaaatatag agcttgccaa
     1441 acccttttag tgagctttac tcattgtacc tttttaggta caatggtatt gcgaacgata
     1501 cgtccgtagg acgtactgaa agggtgggac ttatgagcga acaaaatcaa aatttggcta
     1561 gcgatcattc taaaaaatat acctatcgat ccgagccaaa acaaatcagt tttcgagtga
     1621 gcgaatctga atttgcaaag ctgaagcagt cagctgaagc tttgcaaatg agtgtgccgg
     1681 ctttcgtaaa agccaaggca caaaacgcgc gtctggtgac accgaaagtg gcgccagaca
     1741 ttgcccaggc aatcgcgcgc gatttagcca aagctggagg gaatatcaat caaattgcta
     1801 agtggtgtaa tacacaccaa cacgacgttg ctcctggaga cgcccaacgc ttatccgaaa
     1861 acctaaaaat catgcaaaag gagctacaaa agatatggca acagttaaag taagccgaac
     1921 cacttcttgt agtcgtgcaa ttaattacgc tgaaccacga gcaaccatta aaacaggaat
     1981 aaactgtgac gtcgattatg ccaaaagtga aatgaaacaa attcgcatgc tgtatggaaa
     2041 agacgaccgt gtacaggcac acttattgat tcaatctttt cgaccaggag aaattaccgc
     2101 tgaaaaagct aatcaactag gggcagagta tgcaaaaaaa atagcccccg atcaccaaat
     2161 agctatttac acacatacag acaaagacca tattcataat cacattgtca ttaactccgt
     2221 taatctggag actggaaaaa aatttcaagc tcatggtcaa gcatttctaa acaaatgtta
     2281 tgacctaaat gacgagattt gtcttgcaca taacttacca atcactgagc gaggaaagaa
     2341 accagaaaaa cgaaccatgt ctgaaataaa attaaaagaa aaaaatgaac ctgtttggaa
     2401 agacgaaatt cggattgcga tcgatcaaac aatgaaaaac aaaaaaacca gaacctatga
     2461 ccaattttgt aattccctca aaacattggg gattcattgc ttcaatcgcg ggaaaaattt
     2521 cacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacagactag gaaaggacta
     2581 cgaaaaggag acgattttac atgagctgga cagacgagaa cgaacctata accaacagcg
     2641 aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcagaag aagtacgaag
     2701 agaacaacga actcttacgc ctgaacaacc ggaaattaga ccggctcgaa gaaaccatga
     2761 acaacacgtt gaaagaggaa cagaccacca acaagaatta tcgctctaaa acgttaaaag
     2821 acatagaaaa cgaaataaag aaacaacgtg cttttctcgt tccttatacg caagaaagga
     2881 tacaaactac gattgattta caagttaaaa aactccatca agagctatta gaccagcgaa
     2941 ataaggaaca gaaaaaggtg cttattcaac gatcattgga ttttcttgcc cccttatgtt
     3001 gcttagtcct gacactgttc gcattttggt tagtatgggg aggctaaaat ggcattttat
     3061 ttaccctatc tactcatttt tgttagtatt tcaggaagta tttggctcat ctataaaatt
     3121 tttcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttctttctt
     3181 ggctgtatat tttccttgat aatcgtttca agcggcttat tcggcgtttt agagggaaat
     3241 aagcgcgttt ctagaagtat tttattagga aatgtcactc aaaagtatga atcagcgcgt
     3301 gataaaaaga aaaaagagca agcattagca caaaaaatgg aagagtttac cacttgttat
     3361 aaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa
     3421 aaactcactc gtctttatca aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac
     3481 tatgagcgaa ccaaaaaaga cgtgcaatat gtaaaagata caaagataga agaagcctgt
     3541 agtgatctat ttggagacac aaacccttgg tttgccagtg aagaagaaaa aaaagaaaaa
     3601 caacaatctg tcacatatga acgctacgaa aacctgtttc aacaagcaac taacatgcaa
     3661 aatccgacaa aaaaagaaac cgccttaaac tatctaaaaa gtgtcaagga atggttagat
     3721 cagcaacagc aaaattgaat ttgattgaaa ctcatgttaa actgaatgtg caaatgatca
     3781 aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt tttcaggttg
     3841 atgtcgcctt tactgatttt acttcttgtt tcggttattc aaccaataac tgaataaaga
     3901 gacacaaact cctacaagaa gggggcaaat gaactgttca aacattagat acacctcctt
     3961 ccagtcgcaa gactgtcgga taggcgacaa gttattatac cctacaaaat taaatctgtg
     4021 tggcgatttt aagtatacct tattttcggg ttgttagggc ttgccctgac cgcctgtaag
     4081 gcgcttctcg actagattaa gggagttaat caagaattgt cttagaacgg cttagacaag
     4141 ctctcagatt ctcccacctc ctctttgaac aacggtttta gcaaaggagc cggaggcgac
     4201 tgcgcaggta caatgtgaga gcgtagcgat cacacctttt tagcgaccgt agggagcaag
     4261 ttttctagtt tagaaaaatt cgcgaacctt tttggaggta aaagtaggag ttttttaggc
     4321 gggaaattaa atgtgctctt aagatatact gttagatata ctgttagata tactgttaat
     4381 aataactgtt agggattagc aaccttatgg gagagtaagt attgacatag gtagtggggg
     4441 ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat tgtcacgcta
     4501 tgggggataa atcgtcacac tatgggggat aaattgtatt ttacaccctc ttatgtctat
     4561 gataacataa tttattgagg tgatttttat tgagtaacga tatagttcga tataataatg
     4621 gattaaattc tgtcccttta agaaaattta cccctataga tatggattta ttttggggaa
     4681 tatgttctaa aatgaaaaga aaaggtacaa aagaagaaat ttttacattt aaacaaataa
     4741 aagaattaat tgattacaat ataaatgcac gtggcgaccg ttttgctaat gatttaataa
     4801 aaatgtcaaa aaaaatcatt actttgtctt ttatttatga agacgaaaaa acatatgaac
     4861 aattaaatct gtttcaaaaa tttaaaatcg ataaagtgaa tgaaacatta ttaataaagg
     4921 tttctgaaga atttgaatat atacttaatt caatcggaac taattttact cgtttcgaat
     4981 tagaaaatat gacaaactta aagagtagct acgttaaaga gttatatcgc caacttatgg
     5041 ctcataaaga cagaagatct aaaaaaggtc actggtttgt aaaaatagaa gattttagag
     5101 ctctattagc aataccagaa agttatcgca tgagagatat tgataaacaa atttttgccc
     5161 aagcgaaaaa agaattttta aacgttaatt caactcaccc tgctattttt aaaacttttg
     5221 aaatcaaaaa aattaaagcc tacaaaggaa ataagatagg atctctgcaa atttattttg
     5281 aagaaaatat accttcaatt accttagata attggttaga gaaataataa cctaacaatt
     5341 taggaggcaa ttatcatgca acccaaagaa acctttacaa taaaggaatt atctgaatta
     5401 tttgaaataa gtaggcaagc aatttctaaa catgttgata aattagaccc gtccttatta
     5461 gctaaaaatg agcgtggtta taaggttgta ttacgttctg gagtattgca acttgctagc
     5521 aacttaggta accaaaggtt actagatagg ttgcatgagt atgaacaaga aaacgataaa
     5581 aacgtttata tatcaagaaa aaacgtggtt gtaaacgagt tgctagataa gttgcgtgaa
     5641 gaaaatgaat ttttacgaga acaactttct ctcaaagagc aaaataattc taatatgcaa
     5701 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacagat cgaaaaactg
     5761 caagaacagt tacaattagc ttatacggaa gaacctgaag aagaggtatt aacttctgtc
     5821 aataaagaaa acaacaatgc aataacaaca gaaaaaaagt ggtggaaatt ttggtaacaa
     5881 ttaccaactt tccataaaga agaaatcaat ttctatagac tttatctttc taaaaataaa
     5941 aaatatgttt aggtgatcaa atgactataa gtaaagtaat caaacaaaaa agaatagaaa
     6001 aacaactaac tcaagaagat ttagctgaaa tgcttttagt atctaaaaaa accatttcta
     6061 attgggaaaa tggtagaaca ataccagata cagaaaattt aattcaattt gctaagttat
     6121 ttgatttatc actagacaat attttgttag gagaagaaaa agttatgtta aaaaatcata
     6181 caaaaacact aatactatta tttcattttg taattttttt aagtttagtt tttaccttaa
     6241 ttcagtccaa attcttttat tttagccatt caattgccac ttggttatta atattttttc
     6301 taagtttgct attagtaatc aatactctga ttattatttt taataaacat aaaaatttat
     6361 ttcttcatag tgtttttcca attattttgc tgagtttttt atactttgta taaagtataa
     6421 aaaaagactg ctctttcgag cagccagtta aaaaaataat gagagtttat tcttgaaaaa
     6481 gcaacgcgct tataaagcgc taccaaaatt ttatcatatt ttataaaaaa taacaatcca
     6541 gtaaaaaatg actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa
     6601 gccttaaaaa aagcttttga cctagaccaa tgaaaaatca caaagtgagc agctatagcc
     6661 cttgatagag gggctacttg gcatagtcga cattatgtta actaacgcta taaataacga
     6721 ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg catattgtat
     6781 attttaatta taggtgtata ccacttaaat ttaacacaaa ggcgtgatta ttttgaaacc
     6841 caaaaagaaa atttatctag gtctcctctt ttccttgatg attggaattt ttagtatagg
     6901 aattacaaat acctatgctt cagaaaattc taatttcaat atcaactccc aaccaattga
     6961 agaacaagca attaattttg cgaaaactga ttttgaacac tccataactt caattctttt
     7021 aatggaaaat aacaccgaaa ctgatattaa ctatactctt ggttcgccat ttaaaatgct
     7081 cgatggagaa aatacggcaa atagttctat tttctttcca gtattttcag acgctgataa
     7141 tacgattaaa tatatttata ccgtc
//
LOCUS       DACCPY010000114         6907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCPY010000114 DACCPY010000000
VERSION     DACCPY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.118"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1591)
                     /locus_tag="IVO69_002463"
     CDS             complement(<1..1591)
                     /locus_tag="IVO69_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7401206.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
     gene            complement(1733..1957)
                     /locus_tag="IVO69_002464"
     CDS             complement(1733..1957)
                     /locus_tag="IVO69_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7401207.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2208..3794
                     /locus_tag="IVO69_002465"
     CDS             2208..3794
                     /locus_tag="IVO69_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7401208.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(3860..5635)
                     /locus_tag="IVO69_002466"
     CDS             complement(3860..5635)
                     /locus_tag="IVO69_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7401209.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5848..6750)
                     /locus_tag="IVO69_002467"
     CDS             complement(5848..6750)
                     /locus_tag="IVO69_002467"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7401210.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
BASE COUNT         2034 a         1371 c         1256 g         2246 t
ORIGIN      
        1 ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta tgcaagatag
       61 cttcttctgt tttggcatct actgcagaaa gtgcatcatc taacattaat aattcaggat
      121 ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc
      181 cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca ttgttgataa
      241 atgctaaaga agccgcatgc tttacttctt cttctgaaag ttttggatta gaaaaacgga
      301 tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag ccgattgatc
      361 ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg attttccctt
      421 gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc gcaccagttt
      481 tcccaacaat acctaatgtt tctccttctt ggattgcaaa cttgatttgt tctaatgctg
      541 gtgattcttc tttcagataa ttaaaagagg aaatagctac attcagctcg ccttttgccg
      601 gggtctgcac tgcacccagt tgttccacga tatgcgtttt ttcatgcaaa agttcattga
      661 cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca atcgcaaaca
      721 tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg atcgagatcg
      781 tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa
      841 tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa
      901 ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt ccaaatgttt
      961 taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta gaaaaagctt
     1021 cttgcgaatc acgaaaagca tcatggagtt tcgatcccag cactcgtgag gtgatggcca
     1081 gcaaaggcag aggaatcatc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg
     1141 caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata ccagctcctg
     1201 ctacattctg gatcgcattc aaatcattcg ttgcatgagc catcaaatcc ccagtccgat
     1261 atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc tgccgaagtt
     1321 ctttctccaa ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat
     1381 attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc tttattgata
     1441 aatttttatc ggcaatctca tcaatgatga tcccaatcac ttttggagga actaactgta
     1501 ccaaggccac tgcgaatagc gagaaaactc caattaaata atgtttcttt tcctgacgaa
     1561 aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc
     1621 ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa caaattacga
     1681 aataaaaaac gggctgaaaa aatcagcccg taaaaagtcg ttgggtcaga acctattttt
     1741 tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc
     1801 ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat
     1861 agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa
     1921 taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct ttctatttcc
     1981 gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt
     2041 agtttcatgc aaaaaatagc ggttttttta gaggagaaca aaatttcttt cgtcatctag
     2101 caagacacgc cacgaaaata aattaggcat accgaatctt tttctttaat tttttctttt
     2161 tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat
     2221 ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta tctttagctg
     2281 aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca cttcttgctt
     2341 actctggacc tggtgcgtta gtagcagttg ggtatatgga tccgggtaac tggtctactt
     2401 cgatcaccgg aggacaaaac ttccaatatt tattgatgtc agttattttg atgtctagtc
     2461 ttattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc caaatggatt
     2521 tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta tggatcttga
     2581 ctgaattagc cattatggca acagatattg cagaagtaat tggtgcagca atcgccctct
     2641 acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta gatgttttcg
     2701 ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt gtctgcttaa
     2761 ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca gattggcaca
     2821 gtttatttgc tggctttatc cctaacagcc atacattctc agactcacct gtcattggtg
     2881 gcgaaactcc gctaactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
     2941 tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac gaagaagatg
     3001 tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca atggcttttc
     3061 tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt tctgtccaag
     3121 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
     3181 ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta gcacttcttg
     3241 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat
     3301 tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg ctttctgtga
     3361 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
     3421 ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg ccattttcga
     3481 tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga tttaaaaatt
     3541 cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac ttgaacttac
     3601 gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc gctcaactgg
     3661 cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
     3721 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag gaaacggtag
     3781 aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta ggttaaaaat
     3841 ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt aatttactga
     3901 aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca ccagtgtaga
     3961 cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag acaccgaaaa
     4021 gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa ctataatagg
     4081 cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct gctattgaag
     4141 caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc gcgacttttg
     4201 ctgattttgt taattggaag ttaggtgttc tcggttcatt actatcgatc caattctcca
     4261 ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt ccgaaactat
     4321 aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct gattgaagga
     4381 aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc atcagataga
     4441 actctttttt ctttttgatg gttcccttgt gataggcttc ttctaattct tggttgactt
     4501 ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat tcgcttacta
     4561 catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt ccaccttgag
     4621 tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg aatgtaggag
     4681 atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg ttttctttat
     4741 tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca aattctccac
     4801 tcgaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat tcgatccact
     4861 gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc agccattccc
     4921 gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa gaaaagtctt
     4981 gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa ggaagaagcc
     5041 agatagaatc ttcgcgaatg aaccaaaaaa aaggaaaagc gacagaaagc cctaaaaacc
     5101 atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat aaacaagcga
     5161 taatgatctc aacagccgga atcacgaggg cattacgata cgttcgagtg gaatattcat
     5221 gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga agatacttgt
     5281 tcttcacgat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata ctgaacatac
     5341 cgaataaaac tgcataagaa aaatgcagtt tatgtgcaag tactaaaaat aagctgtagg
     5401 agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg tccaaaatat
     5461 tttgagcata gcgaatcagc aactgatcat cataggttgc cgacatgtct gttccataga
     5521 ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg ccaagaataa
     5581 agtatgtatg tttcttagtc agttggttga tcaagggttt taaagtgttt agcatctttt
     5641 cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgattttt tacttttcta
     5701 ccagtatacc ctgtttgtct aaatttaaaa agagggggga gaagatgcat gatgaaaaag
     5761 actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg tgcctggtcc
     5821 ttttttgctg ctgtcaaata gaaaatctta gagtatcact tcttctgcta ataactcagg
     5881 aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc catatggtgc
     5941 gtaagctttc tttttatctt tgccgacata gggagtttcc aagattttcg aaatattgtc
     6001 aaacattgga tcatggacga tcttattcaa cgtgtcgaat ccgattgttc caaaaccaat
     6061 attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga tatggagtac
     6121 tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg aaaagtcttc
     6181 tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg ataatttttc
     6241 attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac cgatttccgt
     6301 acctttacct gccatcgtct ccaaagcgat ttgtgccgtc tgttcttttg tcaaaacttc
     6361 attcaagcct tcaataattc tagcaattcc ggctgctgtt cctgccccta catgagcacc
     6421 tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct cttctctcag
     6481 aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat taatgatata
     6541 aggggcatga acgacgatat ttgttagacc gttttctgcc atgtacgctt ttccggcatc
     6601 aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg tatagatcat
     6661 aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca ttttctttcc
     6721 gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa ggcaattgtt
     6781 actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga tttttctttg
     6841 ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac tcttttataa
     6901 aaaagat
//
LOCUS       DACCPY010000115         6599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCPY010000115 DACCPY010000000
VERSION     DACCPY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.119"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            132..506
                     /locus_tag="IVO69_002468"
     CDS             132..506
                     /locus_tag="IVO69_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7401211.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IVO69_002469"
     CDS             complement(620..1249)
                     /locus_tag="IVO69_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7401212.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IVO69_002470"
     tRNA            complement(1383..1455)
                     /locus_tag="IVO69_002470"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="IVO69_002471"
     tRNA            1669..1742
                     /locus_tag="IVO69_002471"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            1697..1846
                     /locus_tag="IVO69_002472"
     CDS             1697..1846
                     /locus_tag="IVO69_002472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401213.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2339..3667
                     /locus_tag="IVO69_002473"
     CDS             2339..3667
                     /locus_tag="IVO69_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7401214.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="IVO69_002474"
     CDS             3869..4702
                     /locus_tag="IVO69_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7401215.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="IVO69_002475"
     CDS             4718..5455
                     /locus_tag="IVO69_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7401216.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="IVO69_002476"
     CDS             complement(5610..6578)
                     /locus_tag="IVO69_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7401217.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
BASE COUNT         2175 a         1076 c         1293 g         2055 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaat
//
LOCUS       DACCPY010000116         6597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCPY010000116 DACCPY010000000
VERSION     DACCPY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.120"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..662
                     /locus_tag="IVO69_002477"
     CDS             <1..662
                     /locus_tag="IVO69_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP7401218.1"
                     /translation="VSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQR
                     SIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
     gene            complement(748..1842)
                     /locus_tag="IVO69_002478"
     CDS             complement(748..1842)
                     /locus_tag="IVO69_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7401219.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2031..2792
                     /locus_tag="IVO69_002479"
     CDS             2031..2792
                     /locus_tag="IVO69_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7401220.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2907..3347)
                     /gene="fabZ"
                     /locus_tag="IVO69_002480"
     CDS             complement(2907..3347)
                     /gene="fabZ"
                     /locus_tag="IVO69_002480"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7401221.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3567..4322
                     /gene="fabI"
                     /locus_tag="IVO69_002481"
     CDS             3567..4322
                     /gene="fabI"
                     /locus_tag="IVO69_002481"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7401222.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4432..5445)
                     /locus_tag="IVO69_002482"
     CDS             complement(4432..5445)
                     /locus_tag="IVO69_002482"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7401223.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5610..6530
                     /locus_tag="IVO69_002483"
     CDS             5610..6530
                     /locus_tag="IVO69_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7401224.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2035 a         1308 c         1176 g         2078 t
ORIGIN      
        1 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
       61 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      121 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt
      181 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      241 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      301 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      361 ttacgtttga ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata
      421 ttgatatata ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta
      481 acgggattct gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga
      541 catttatttc cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca
      601 gaacaccaat cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt
      661 gacaaatcat aacctataaa aaaacaaaat aaaaaagcag aaataactgg ctatttctag
      721 cggattattt ctgcaataac taattatcta ttttttattt tcaattaatt taaagcttga
      781 ccatggtttc aaaaagtcaa ggaggaataa ttcgtcatcg gagattcttc ctacaacatt
      841 gacacgtccg tcgtttttca tatccttcaa ggcgatttgt gtttctcctt tgtattgacc
      901 gtacccttca ttgtcaatca gtacatcccc atgatgcata tctcgcgtat tatgaggcgg
      961 gaaatcttta tctttgtagt atacacgagt catcgttgaa cgaaggatat attctgagcg
     1021 atcccctcga taactatgaa ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc
     1081 ttcttccgta tctactttca atgttggata atccgcgtta aatgcttctg ccatttcttt
     1141 caattcttct tcacttgcgt acgcatttcc aatagaaata tcatcaatca atcctgtaag
     1201 gattaaatgc ttcacttgtg tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa
     1261 gccatcttgc gtaggccaag ggccaaactc agcagattgc gaattgacga atgccatcgt
     1321 attcaaatta tattttctga atttttctga acaaaaaaca aagtgttcgt atcctaaacc
     1381 agagtatcga tgaggataaa aattatgaga acctaataaa ttatccgtgt ttggcgaata
     1441 actcatgatg ttgtcaacgt aattcgtgcc cgaactcata ttgatctcga ttttgatacc
     1501 aaaaggattg cgcgtcattt ttgcttcttc tgctcctgtg aatccgatat ctaagcggac
     1561 cccgtaggca cccatcttat ggaaaaaaga taagtcatcg taagaaatct ccaactgttc
     1621 aaataacgct ggattgatat caaccattac ttccatgcct aggctattcg cgtaatcaac
     1681 tacttcttta aattctgaaa gaactttttc tttatcatca ttgatctgca gcaaactggt
     1741 aaaaacccgt ttatacccat acttatgagc taaatccaag tacgctttgt ccttttcaaa
     1801 tgttgaccgt tctggataaa tcgagattcc taattttccc atattaccat ttcctcctgc
     1861 atcttttcgt ttttgtatcc acttacatta tagcaaacac aaagtaaatg gtctacacct
     1921 tttctttttc ttttaaaata aatgataaat tccaataatt taagtaggta ttttccctct
     1981 attttgttat cattaaagat aacaaaatag taaattggaa aggaacagtt atgaagaaaa
     2041 ataaagttat tttaagtatt agtttagcag cagcattgct gtttgctgct ggttgttctg
     2101 caaacaacac gacttcttct agttcgtcta cagaaagttc aaaaagtaca tctactgcgg
     2161 aaagcacgac tgccagtagc gtcgatttga acgcattgga attaccacaa ttgacgaaag
     2221 atgtcgccga aaatgaagat ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca
     2281 aattatttcc cgaacacgca ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg
     2341 gttactacaa cggactgact ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg
     2401 atcctaaagg cgatggcact ggaggggaaa gcatttgggg agaaggattt gaaacagaga
     2461 tcaacaacca cctttataat ataagaggtg cgttatctat ggctcgctca caagatccta
     2521 acagtaacgg cagccagttc tttatcgttc aaaatacaga tgatatgcat gatggtcttt
     2581 taaaagacga ctatcctcaa gcaatcatcg acgcttataa aaacggcggt tatccttcac
     2641 tagatggcca ttatactgtc ttcggccaag tgattgaagg aatggatgtc gtggatgcta
     2701 ttgcaaatat cgaaacagat agttcagaca aaccaaaaga agaagtaaaa atcgataaaa
     2761 tcaatatact gcaagaagca aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat
     2821 gacaacgtat gctaggattc aggcgtcttc tttttacata aaaagcaagt aagcttcctt
     2881 ttgttttgct atatcaggtt ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg
     2941 cactcacaga cttttttatc atctacatat gctgcagata acgctgtacc gactgatccc
     3001 ttcattttgg tgatttcaaa atgacatttc agcacatctc ctggaacaac tttttgacgg
     3061 aatttggcct tattgatccc accgatataa gctctttttc ctagaaactc ttctgatttt
     3121 aggataagta tcgaacctgc ttgtgccagt gtttctagaa tcaacgcacc cggcatcact
     3181 ggattgtcag gaaagtgacc ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca
     3241 acgatctttt ttcctggttc taaagtatct acataatcaa tataacaaat aggataacga
     3301 ttaggaatca attccattac ttcagtagca gataatacgt aagacataat taaactcctt
     3361 ctttcaggca attgccattt tcattacttt gactatcaaa gtatcatatt cctaaatatt
     3421 tagcaatctt tttttaattt aagaaaaaat agtttgacaa tcaaaacatt ttgactaata
     3481 attaagaaat caagaagtta ttttagctat tattctctat ttttttacga ctatcaaatt
     3541 tttatcagaa acaaaggaga gtcattatgt catttctaga aggaaaaaaa atcgtcatta
     3601 tgggcgtcgc taacaaaaaa agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag
     3661 gggcagaaat tatctatact taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg
     3721 tcggcgaaga agcattcact gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag
     3781 caatgaatgc catcggtgaa tatgctgggg agatccacgg ccttgtccat gcagttgctt
     3841 acgccaacaa agaagaattg tccggaaatg tgtctgatat ttccagagac ggctatcaat
     3901 tagctcaaga tatcagcagc tactctttaa ttgctgtatc aaaatacgca caaccgtttc
     3961 ttgcaccaaa cagcgggatc gttagcatga cttatctagg ttcagaacgc gcaatcccga
     4021 attataacat gatgggtatt gcgaaagctt ctcttgaagc tgctatccgt tatttggcag
     4081 ctgagttctc gcctaaagga attcgggtca atggaatctc agctggggca atcaaaacat
     4141 tagctgtcac aggtgttaaa gactatcaaa aactgatcca gttatctgaa tctcgtacgc
     4201 ctgatcagca aggtgtgacg atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc
     4261 cactttccag cggaatcatc ggtgatatta tctacgttga caaaggtgtc catctttcat
     4321 aaacttttgt tgcattcttt aaagtaaaac atataaaaat caggaaaaaa ctttttaaag
     4381 tctcttcctg attttttctt tttacgtttt tttcaactca ttactttgaa attattccac
     4441 gacttcaatc cagccttctg gtgcttctat gtctccaaat tgaatacctg tcaattcatc
     4501 atacaaccgt tttgttactg gtcctacttc cgtttcacta taaaatacat gaagattgtt
     4561 attgtgataa atcccaccga ttggagaaat aacagccgct gtaccacatg caccagcttc
     4621 tgcaaattga tctagatcat caatgaaaat atctccttca attgcttgca gtcctagtcg
     4681 ttcttcagct aactgcaaca aggaatattt cgtgatactt ggtaagatag agggcgattg
     4741 cggagtgatg aattttccgt cttttgtaat tccaaagaaa tttgctgcac ctacttcttc
     4801 gattttcgta tgtgtggctg gatcaagata aacacaatct gagtaatcca gttcttttgc
     4861 ttctgttcct ggaagtaaac tcgctgcgta atttccgccg acttttgctg caccagtccc
     4921 cttaggagca gcacgatcgt aatcagatac aataaaattc gttggtttta gtccaccttt
     4981 gaaatacgct cctactggcg tacagaagac gacaaataaa tattctggag ctggatggac
     5041 accaatgttt tcaccgaccc caatcagtaa agggcgcaag tataatgtag cgcctgttcc
     5101 atagggcggc acaaaatctt catttgcttt taccacttgc ttgacagcat caacgaacat
     5161 cttttcaggg accattggca tcagcaatcg tctagcactt tgattcatcc gtcgactatt
     5221 ttgttcaggt cgaaagagat tgaccttgcc gtcttttcga cgataagctt ttagaccttc
     5281 aaaacattgc tgaccataat gaagcgctgg agagccttca tgaatcgtca ggtaattatc
     5341 ttctgtcaat tctccttctt cccattgtcc gtctttccat ttggcaataa aacgccaagg
     5401 agttttgata taagaaaaac ctagattgtt ccaatcgatt tgcatatact taccaccttc
     5461 gttattttct ccacatttta tcattatcca tattatttgt cattttattt tcagcttttt
     5521 tccttcatta tagttatttt tgctatttcc ttttttgaaa acttcttggt acaatacaga
     5581 aagaatcaaa ttttagaaaa gaggatttta tgttttcatt attagcaact gccggtacga
     5641 cagactcttc tggcgagaca ttgacagatc aagctgtcag caaagtaaat gcgtttcagc
     5701 ggttttggga cgggattgat tgggacgcca ttttggctgc ctttatccaa aaaagtttat
     5761 atctgatttt tttgatcgtc cttttttgga ttcttcaccg tgtgggaaaa tatctgattg
     5821 ataagtcttt taatcagtat tctaaaaaag ccattctaaa cgagagccgc ttaaagactt
     5881 tacatagtct tttaaatact atttttcatt atacccttgg tttctttttt atctatgcaa
     5941 ttttatctgc tatcggtgtg ccgatcggat cacttttggc tggtgccgga attgcaggtg
     6001 tggcaatcgg tcttggtgcc caagggttca tgagtgatgt tattaccgga ttttttatta
     6061 tcatggaaca acagatggat gttggggact acatcaagct agcaaactta acgattgaag
     6121 gaaccgtcgt atctgttggt atccgtaccc ttcagctgaa agcaacggat ggtactgtcc
     6181 atttcatccc gaatcgaaac atcaccacga tcagcaatct ctctcgtgca aatatgcaag
     6241 tacttttaga tattcggatt gttcctgaag aaggatatga caaaatctat gagatcattg
     6301 atcgagtaaa tcgtacatta gctgaaaaat accaagaaga gctccaaact gaaccttcta
     6361 tctttggatt agtggatctt ggaaacagca attttgcgat tcgtacggtc tgctacgcat
     6421 tgaacggaaa acaatatgcc ttaaaagaag agttcttgag ctcctatgtc aaagaattga
     6481 cagcttctgg tttcacgatt ccgaacagtc caattgctgt cggaaaataa tttggacaaa
     6541 cgatgcaacg aatctcacta aaaagagcgt gggacaaaag taaagaacac ttttgtc
//
LOCUS       DACCPY010000117         6436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCPY010000117 DACCPY010000000
VERSION     DACCPY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.121"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(153..1193)
                     /locus_tag="IVO69_002484"
     CDS             complement(153..1193)
                     /locus_tag="IVO69_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7401225.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1542..1868)
                     /locus_tag="IVO69_002485"
     CDS             complement(1542..1868)
                     /locus_tag="IVO69_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401226.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1855..2643)
                     /locus_tag="IVO69_002486"
     CDS             complement(1855..2643)
                     /locus_tag="IVO69_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7401227.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3011..3301)
                     /locus_tag="IVO69_002487"
     CDS             complement(3011..3301)
                     /locus_tag="IVO69_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7401228.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3437..3616)
                     /locus_tag="IVO69_002488"
     CDS             complement(3437..3616)
                     /locus_tag="IVO69_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401229.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3613..3963)
                     /locus_tag="IVO69_002489"
     CDS             complement(3613..3963)
                     /locus_tag="IVO69_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401230.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3956..5281)
                     /locus_tag="IVO69_002490"
     CDS             complement(3956..5281)
                     /locus_tag="IVO69_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7401231.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(5652..6236)
                     /locus_tag="IVO69_002491"
     CDS             complement(5652..6236)
                     /locus_tag="IVO69_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401232.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1942 a         1171 c          928 g         2395 t
ORIGIN      
        1 ttaaaaccta cgattgtgat tgttttcata aacttttaat ctaaaaactc cggaatacta
       61 actatttatt tgtaatattt gtaataaaat taaaatcata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtaatt gctttttatc aactactcta accaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcgacaaacc aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca
      301 gttttttgtt tttgataagc tttcagtaaa gtcatatata atcctttgtc agcatcaatt
      361 ccataagatt ccagttcttc ataaacgaca atagtttgct ctaattcttc agcttttttc
      421 tttgcattat gaatagtttg gacggtttgt tgagcttcac tgtaacttga agaaaatgtt
      481 tttatcaagt ttaatacatt ttctggtaca aatgtattta tactgtcctc ttgcatgatg
      541 tcgacgaagt tatctagcaa aacttgattt tgtaactggt cagtttcagt gtctattatg
      601 tgtctattag tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg
      661 gttgagtcct tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtagaa
      721 tcattgattt ttcgcggttt tgccgtattt gcgattttcg caattacggt ggaatcagta
      781 ttgtcttcct cttccatgat tttaaagacg tcttgattcg taacttctgg cttgcctaag
      841 tacaataaat taggcataga ttcaatctta ccatcacgtt tatttactct tcctctcttc
      901 tgtttcagaa gacctgcttt ttctaattct tttttgatct tagatacttt accttcgcgg
      961 cattgtaaca atttcattaa ttcttcaact gtgaatatga agaatatatt atcatcagaa
     1021 tctacccagt tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt
     1081 tttgcatcat ttgaaagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga
     1141 taatattttt cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat
     1201 cctctttctg taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata
     1261 tctatccact atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt
     1321 ctttttttat ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc
     1381 agggtttaag ctcatggtaa ctatatcacg atattacaat aacattatat cacgatatcg
     1441 taatattaca atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa
     1501 aaaatatcac gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc
     1561 tttgctagtt tatatccttc aatcaagtat tcttcaggta tggattttaa tagcgcatct
     1621 tctataacgt ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct
     1681 gacattagaa ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct
     1741 tttatgactt tacttatatt tttcttggtt aattcgattt cttctacttc ttttggtttg
     1801 tagttctttt tatttttatt tcctagtagt ctacttactg cagcttgact gcgattatct
     1861 tctgccatca aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc
     1921 ttcagctgtt gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc
     1981 aacggttatt gttctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat
     2041 ttcggttgct gcagtcgtca tttctttaga aatgttataa cgcttattat acttagtaaa
     2101 taggactcct cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc
     2161 acttaacttt attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc
     2221 atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat
     2281 aatataatca tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc
     2341 tacttgagta aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat
     2401 tcctttatag tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat
     2461 tgtaggttgg tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg
     2521 atcaatgtcc actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt
     2581 aacgctagtc gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc
     2641 cattaaaata tcccccattc atcttatatt ataatataat tatatcataa tattgtgata
     2701 ttgcaatatc gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat
     2761 atatcggttc ggatataaag attattcata tgttattcga aagtaaaata atcacttttt
     2821 attactttaa aattttttat taatcataag aaatatgttt tgcgagcagc aactagaata
     2881 atttaaacat tattaaactg tctatattgt tactaataat tcccttctta tagtgcattt
     2941 tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt
     3001 gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat
     3061 attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca
     3121 ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc
     3181 agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc
     3241 ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca
     3301 tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat
     3361 tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata
     3421 gaatccgtaa aataccttat atatataacg ggaaaatata aaacctaaaa ttatgataac
     3481 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     3541 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     3601 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     3661 tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa
     3721 gatcttgcca acaatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat
     3781 agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt
     3841 catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc
     3901 agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat
     3961 catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc
     4021 ttctagcgct ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc
     4081 gaatcttgtc aataatttta tctaaatttt cttcgttaat ttgcttttgt ggatcactaa
     4141 ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacgtgcc
     4201 ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     4261 ttgtattatc tgtgataggt attttcatct ggtgtgaaaa tcctggtctc gtttctaaga
     4321 tcgacgtacc aatgtttaaa tgaacacatc ctgttttaca attatgctgc ctgatacgaa
     4381 tggctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     4441 ctcgtggtaa cacttgtgaa ttaccataag atttttcctt tgtcggttct ggcgggattg
     4501 ctatatctgt tcgatcgatt ccgttagcat ggaagtacag ctgtaatcca atcacaccta
     4561 atctattctt aatcgtataa ggattccagt tagcaaggtc tctaatgctc aaaattccca
     4621 tttggttgag cctttttttc attcgagaac caattcccca aaagtccgtc atttcgggaa
     4681 tattccatac tttctcagga acgttttcgt acgtccattc cgcaataaat ccttcattat
     4741 gctttgcttc attgtctaag gctaatttag ccaaaagggg attatcgccg acgcctaccg
     4801 tagcaatcaa acctaactct tctttaatac gttcttggat catttgagcg agcttcttcc
     4861 ttcgctgact tcgtgtttct tcagtcgtaa aaagattcag tgattttgtc acttttaaaa
     4921 tcgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt ctgaaaatat
     4981 tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca acgtgtagtg
     5041 ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata ccataccgct
     5101 tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca gatcgactca
     5161 taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg acacttgcgt
     5221 aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa taatccatca
     5281 taaataacac tccattcttc cacattatac aaacatatgt tcgatgttgt aaagaagaaa
     5341 tagctaaata cataaaaaaa gaagcatagg tatgctatca ccctgcttct tcttatgatt
     5401 tgtatgatca atttattcta tcactttact aattaatttg aaaatctaat aaaaactctc
     5461 actctaaaag agtgagagac tgcaaattag agtacggact tttttggagt tccgttcttt
     5521 aatattaaca aatgaataaa atccttacaa catttgataa taagttatca gtagacactt
     5581 acaaaaaatt ctcatactat tgtcactttt ctagataaat ggcaaaaatt ttcagcatta
     5641 aatgacaaaa actaatgaga atttgtcttt ccatttttct cctccatttt tgccaaataa
     5701 cgataaatgg tggttcttga gacgttccac cgttccgcaa tagtcttaat aggtgtaccg
     5761 ctatccacta aagtcttcag taattccaaa tcttgttcag acaatttttc aggacgacca
     5821 ccaaaccgtc ctcttgcacg agcagctgct cgtcctgctg cggatcgttc caggatcaaa
     5881 ttacgctcaa attctgcaaa tgccgcaaac aaatgaaaca tgagctgtcc tgtagaactt
     5941 gatttgtcca tcgtaatatt ttcttgaata ctatgaaaac tcacaccacg ctcattcagt
     6001 cgattgacga ttgaaatcaa atcttccata tttctaccta aacgatctaa gcgccaaacg
     6061 acaagtgtat cgccagagcg aacaaaatca atggccattt ctaatcctgg tcgattactt
     6121 tttgccccac tcatatgatc agtaaaaatt ttttcacaac caaatttatt taggctgtct
     6181 tcttgtaaat ccaaattttg taaaccagta gatacccggg cgtatcctat tttcaaaaaa
     6241 atcactcctt cattaatttt attgtaacat aactttatcc atatagttta actgaacata
     6301 gatattgtta tgggtttatg aacatgaaat gaattgtttt tttgaaaaat aaagcaaaaa
     6361 aaggaagtgt ccagaaatgg gtgattttat gaacactttc aattagaaaa aaagaatgtc
     6421 gactagacac tctttt
//
LOCUS       DACCPY010000118         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCPY010000118 DACCPY010000000
VERSION     DACCPY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.122"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IVO69_002492"
     CDS             725..811
                     /locus_tag="IVO69_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7401233.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVO69_002493"
     CDS             complement(932..1621)
                     /locus_tag="IVO69_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401234.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVO69_002494"
     CDS             complement(1628..1906)
                     /locus_tag="IVO69_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401235.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVO69_002495"
     CDS             complement(1866..2780)
                     /locus_tag="IVO69_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7401236.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVO69_002496"
     CDS             complement(2762..3142)
                     /locus_tag="IVO69_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7401237.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVO69_002497"
     CDS             complement(3592..3879)
                     /locus_tag="IVO69_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7401238.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVO69_002498"
     CDS             complement(3899..4123)
                     /locus_tag="IVO69_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7401239.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVO69_002499"
     CDS             complement(4763..5296)
                     /locus_tag="IVO69_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7401240.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVO69_002500"
     CDS             complement(5317..6159)
                     /locus_tag="IVO69_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7401241.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCPY010000119         6112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCPY010000119 DACCPY010000000
VERSION     DACCPY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.123"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            58..510
                     /locus_tag="IVO69_002501"
     CDS             58..510
                     /locus_tag="IVO69_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401242.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="IVO69_002502"
     CDS             524..1213
                     /locus_tag="IVO69_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401243.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="IVO69_002503"
     CDS             1338..3689
                     /locus_tag="IVO69_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7401244.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="IVO69_002504"
     CDS             3769..4164
                     /locus_tag="IVO69_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401245.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="IVO69_002505"
     CDS             4489..5106
                     /locus_tag="IVO69_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401246.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="IVO69_002506"
     CDS             5147..5446
                     /locus_tag="IVO69_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401247.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="IVO69_002507"
     CDS             5480..5647
                     /locus_tag="IVO69_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7401248.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5692..>6112)
                     /locus_tag="IVO69_002508"
     CDS             complement(5692..>6112)
                     /locus_tag="IVO69_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401249.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2110 a          837 c         1226 g         1939 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     5701 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     5761 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     5821 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     5881 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     5941 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     6001 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     6061 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg at
//
LOCUS       DACCPY010000120         6077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCPY010000120 DACCPY010000000
VERSION     DACCPY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.124"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVO69_002509"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVO69_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7401250.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IVO69_002510"
     CDS             complement(2203..4389)
                     /locus_tag="IVO69_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7401251.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVO69_002511"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVO69_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7401252.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IVO69_002512"
     CDS             complement(4611..5051)
                     /locus_tag="IVO69_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7401253.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IVO69_002513"
     CDS             complement(5125..5664)
                     /locus_tag="IVO69_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7401254.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1576 a         1368 c         1068 g         2065 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgagg
//
LOCUS       DACCPY010000121         6029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCPY010000121 DACCPY010000000
VERSION     DACCPY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.125"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(125..772)
                     /locus_tag="IVO69_002514"
     CDS             complement(125..772)
                     /locus_tag="IVO69_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7401255.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1180..1683)
                     /locus_tag="IVO69_002515"
     CDS             complement(1180..1683)
                     /locus_tag="IVO69_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7401256.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="IVO69_002516"
     CDS             complement(1806..2570)
                     /locus_tag="IVO69_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7401257.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="IVO69_002517"
     CDS             2678..2953
                     /locus_tag="IVO69_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7401258.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3154..3501)
                     /locus_tag="IVO69_002518"
     CDS             complement(3154..3501)
                     /locus_tag="IVO69_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401259.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3669..4598
                     /gene="yidC"
                     /locus_tag="IVO69_002519"
     CDS             3669..4598
                     /gene="yidC"
                     /locus_tag="IVO69_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7401260.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4701..6029
                     /locus_tag="IVO69_002520"
                     /pseudo
     CDS             4701..6029
                     /locus_tag="IVO69_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1870 a         1154 c         1109 g         1896 t
ORIGIN      
        1 aaggggctgt ccccagactt tagtaggcgt cattagctat ctttcgttag catttaaagg
       61 cgaacaccat cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat
      121 aaaattatcg ttctttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta
      181 actgttgatt acattggtca ctatcatgac aaatataatt cccctttttc gtatacgtgc
      241 catcagaacc tgctttcgtt gtagaaagaa aaagcgaaac attcgatgtt ttctgacaga
      301 ttgcacagat tcccttattt atcgttggtg ataaagtacc gctaatccct tttaattttt
      361 catcttcgta atagaccatg aattttttct gcgtacctgg atcattccag ccgatgtacg
      421 tatgttcttg caagtcgatt tcattccaag ctggttgttt gagttttttc acttttcgga
      481 ataatttctc gatttgtggt ttgctaggct gcataaacgg ctgaacgatt tgcttcagct
      541 cctccaatgc tctttctgct tgcgcttttg tcagttttac gtctaaaact gtcgttaaaa
      601 atgtatcgat ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcctgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag cttgcatctg cagtaataag ccgaaacttc
     1081 acaaaaattt gagaagtaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttacctgt taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgcagacatg ggctgagccg cttctttgac
     1261 tgcgcagtat ttgtccacga atgtcacaat atatccttct ttatacttag gcggtgcgat
     1321 cgttgcaccc cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat
     1381 tttttctgca ttctttacag caattcttgg atggatatac gcatgtgtcc cctcgtcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttgta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgct ctagataaaa tagctaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaacg atccctgctg cgactccgac attcaatgat
     1861 tccgcatttc ctttcattgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 ctgactccct gaccttcgtt tcccatgatt atagcgaaat cctctgtttt ttctactttg
     1981 tctaatgcat gtgcttcttc atttaattct gtaccgtaca ccgtaaaccc tttgcctttc
     2041 attacaggga ttacatctgc caaatctttt tgtaacacag gcaaatgata attgcttccc
     2101 tgcatgcttc gcagcacctt tgtactataa atatctgccg ttcctttacc taataaaaca
     2161 ccagccatac caaacgcatc tgccgtacgg atcatcgttc ccacatttcc aggatcctgt
     2221 acattatcta gtaaaagcca gcctcctgaa aaatctggcg tatcttcaga tgttaacgtc
     2281 atcactgcta agatcccttg cggtgtaggt agatctgaca tagcggtcat gacctcatct
     2341 gaaactagaa aagtagaaat ctctttgttt acagccagcc agtctcccca ttctttcata
     2401 cctctctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcgatc
     2461 agatggaacc cttccaaaag atattctgcc ttttcttctc tgtatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attccttcac
     2581 ctcacctgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattgattt ataaaacagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaatgt
     2701 ccaaggacgt gtccagggcg tgggatttcg ctatatgacc aagatggttg ctgatcagtt
     2761 aggcgtgacc ggaacggtaa aaaacgaaga cgacggctca gtcacaatcg aagcaatcgg
     2821 aaatgacgac atcatccaaa aatttatcga agaagtcaag aagtctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga tccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaggtcgc ttagttttga gtattaagga aaattttgtg
     3001 aaaatggctt tccacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa
     3061 caccgtcgat aaggttgttt ttgaagcgct ttgcctcgag tattaaggag aaacattttc
     3121 aaaatagctt ctcatatttt gaaatgtaat gacttaatac cgagaggcag ctgataaaac
     3181 accgtcaata aggttgtgaa aaagctggtc tgcatcaagc attaaggact ggttgtgaaa
     3241 aagtcgctta gttttgagta ttaaggaaaa ttttgtgaaa atggctttcc acatttttgc
     3301 ataaatttgt cttaatacca agaaactgac ttttgaacac cgtcgataag gttgtttttg
     3361 aggcgctttg ccccaagtat taaagacagg tagtaaaaaa gctgatctat cacttgcagt
     3421 gagttcattt gactcctcta tttcgggcag aataaaaaaa gtttttagtt ttttaaataa
     3481 gaaagaaaca cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct
     3541 tttttcaaaa tttttcgcta cgaaaaagaa agaatattta tatgaaattt ctatgaagtt
     3601 gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga
     3661 agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc
     3721 tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg
     3781 tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca
     3841 attggtctta cggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc
     3901 cactaggtat cagtcaatca aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa
     3961 aaccacaagt tgaagcagca caacaaaaac taaaagctgc aacaacacgt gaagaacaaa
     4021 tggcagcaca agcagaaatg caacaagtat atcgcgaaaa cggattgagt atgaccggcg
     4081 gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg
     4141 ctcgctacac agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa
     4201 gtatggtgct tgttgccatt gcaggggtcg cttatctgct tcaaggatat atttcaacaa
     4261 tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta
     4321 tgatcgtttt catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtgggtg
     4381 gtgtgttcag ttgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag
     4441 cacaagtggc agaagagttg aaacaaaatc cgccaaaaca agtcgtcact ccaagaaaag
     4501 acgtgacaga atcggcaaag ccagctgaca ataaacaacc aaaaaacagt acaaacaaca
     4561 aaggccgcaa cgcaggaaag cagcaaaagc ggaaataaaa atatatgaca aaaaactgta
     4621 gacaaataaa aaaatttctt tgttctacag tttttttgtt acaatcaaag aaaacgtcgc
     4681 ttttcgagga ggaatgtata atgatgtcca aataatcaat ggatagtcga atggaagaag
     4741 ccaaaatttc gctagatgca cttgttccta aaaatcattt ggtccataaa attgacaaaa
     4801 tattgttatt tgattttatt tatccaattg ttgaatcaac ttactcgacc attggtcgtc
     4861 ctagcgttga tcctgtcatc ttagtaaaat tagtattcat tcaatattta tttaggattc
     4921 gttcgatgcg tcagtccatt aaagaagctg aaacaaacct agcttatcgt tggtttcttg
     4981 ggcttagttt aatcgataaa attcctcatt tttcaacgtt tggtaaaaac tatgtacggc
     5041 atttcagaga aaccgattgt ttgaacagat atttatcatt atccttgaac aagcagttga
     5101 agctagattt attcatgaag aaattttgta tatggattca acctatatta aagcgaatgc
     5161 gaaaaaacat atttttacaa gagaaatggt acataaataa gccattttat accaaaaaca
     5221 attgaaagaa aaaattaatg aagcaagaat tgcagaaggc aaacgtcctt ttggttcaat
     5281 cattcatcag gaggtggtag aagggaaaat tagtgtcgca gatccagaaa gtggttatta
     5341 tgtaaaaaca gaacaagaaa aacagtttgc ttattctgct cataccgttt gtgatgaaaa
     5401 tggttttgta ttagatgtca tgattacgcc tggaaatcta catgacagcc gaatgctcgt
     5461 gccacaaatc gaacgtgtta aatcatgttg tggtgtagct gctgatgctg cgtacaaaac
     5521 accttggaac gccaagtatt taatcgatcg aaaactacga cccatttttc cttatactcg
     5581 acccaaaaga agcaaagaac gttttaaaaa gaaagatttt tattatgatc cctattataa
     5641 ctggtaccta tgcccaaatg atcaaacact tcagtttcgc acaactactc gagatggtta
     5701 taaaaaatat gtatctaaat cttttatttg tgaaagttgt ctattattga aaaattatac
     5761 agaaagccag aaacaccaga aagaaataca tcgacatatt taggaagact acttagatga
     5821 agcagaacat ttacgccata cggtgtacaa tcgcgaaaaa tataaaaaaa agaaaagaaa
     5881 cgattgaacg cgtttttaca gacgcaaagg aaaagcatgg aatgcgctac accaaatatc
     5941 gaggcttgga aaaaaccagg tgcatacgat gcttgttttt gccgcaatga atctgcaaaa
     6001 attagccatt tggcagtggg ggcatatgt
//
LOCUS       DACCPY010000122         6011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCPY010000122 DACCPY010000000
VERSION     DACCPY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.126"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            54..1322
                     /locus_tag="IVO69_002521"
     CDS             54..1322
                     /locus_tag="IVO69_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP7401261.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1330..2736
                     /gene="lpdA"
                     /locus_tag="IVO69_002522"
     CDS             1330..2736
                     /gene="lpdA"
                     /locus_tag="IVO69_002522"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7401262.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2898..3173
                     /locus_tag="IVO69_002523"
     CDS             2898..3173
                     /locus_tag="IVO69_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7401263.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3179..3946
                     /locus_tag="IVO69_002524"
     CDS             3179..3946
                     /locus_tag="IVO69_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7401264.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4114..5943
                     /gene="typA"
                     /locus_tag="IVO69_002525"
     CDS             4114..5943
                     /gene="typA"
                     /locus_tag="IVO69_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7401265.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         2055 a         1065 c         1348 g         1543 t
ORIGIN      
        1 cagaatcagc tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg
       61 aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg
      121 aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg
      181 tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg
      241 tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag
      301 ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg
      361 atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg
      421 tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg
      481 aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg
      541 cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat
      601 tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta
      661 tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag
      721 tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg
      781 gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat
      841 acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact
      901 acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg
      961 cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc
     1021 atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg
     1081 gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag
     1141 gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc
     1201 gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac
     1261 aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat
     1321 gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg
     1381 ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc
     1441 attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca
     1501 ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact
     1561 agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc
     1621 catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa
     1681 ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa
     1741 acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga
     1801 tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct
     1861 aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca
     1921 aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa
     1981 aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg
     2041 aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg
     2101 gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg
     2161 aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc
     2221 acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt
     2281 ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc
     2341 tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct
     2401 gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa
     2461 gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga
     2521 ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga
     2581 gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct
     2641 gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct
     2701 tcggaattag cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca
     2761 actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt
     2821 aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg
     2881 aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag
     2941 acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga
     3001 taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg
     3061 gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta
     3121 cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat
     3181 ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc
     3241 ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt
     3301 cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa
     3361 aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat
     3421 catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat
     3481 gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa
     3541 tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag
     3601 accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca
     3661 taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg
     3721 cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct
     3781 tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag
     3841 cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc
     3901 ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa
     3961 tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat
     4021 tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt
     4081 cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac
     4141 gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa
     4201 caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg
     4261 ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt
     4321 attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg
     4381 atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct
     4441 caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta
     4501 aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta
     4561 ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca
     4621 ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct
     4681 atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta
     4741 caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt
     4801 gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca
     4861 gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt
     4921 caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt
     4981 gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca
     5041 acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc
     5101 gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta
     5161 cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat
     5221 ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca
     5281 gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc
     5341 gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa
     5401 atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc
     5461 cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg
     5521 aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa
     5581 ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa
     5641 gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg
     5701 gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac
     5761 gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg
     5821 gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt
     5881 cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa
     5941 taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt
     6001 tttgtccaac t
//
LOCUS       DACCPY010000123         5964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCPY010000123 DACCPY010000000
VERSION     DACCPY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.127"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..120
                     /locus_tag="IVO69_002526"
     CDS             <1..120
                     /locus_tag="IVO69_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7401266.1"
                     /translation="RLDAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            132..602
                     /locus_tag="IVO69_002527"
     CDS             132..602
                     /locus_tag="IVO69_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7401267.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            619..1398
                     /locus_tag="IVO69_002528"
     CDS             619..1398
                     /locus_tag="IVO69_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7401268.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1385..2206
                     /locus_tag="IVO69_002529"
     CDS             1385..2206
                     /locus_tag="IVO69_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7401269.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2223..3272
                     /locus_tag="IVO69_002530"
     CDS             2223..3272
                     /locus_tag="IVO69_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7401270.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3285..4289
                     /locus_tag="IVO69_002531"
     CDS             3285..4289
                     /locus_tag="IVO69_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7401271.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            4922..5941
                     /locus_tag="IVO69_002532"
     CDS             4922..5941
                     /locus_tag="IVO69_002532"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7401272.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         1969 a          976 c         1306 g         1713 t
ORIGIN      
        1 cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa
       61 aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag
      121 gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag
      181 ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac
      241 ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag
      301 tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata
      361 agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag
      421 aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa
      481 aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa
      541 tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt
      601 aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc
      661 gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg
      721 tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag
      781 ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt
      841 gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta
      901 ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg
      961 attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc
     1021 gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg
     1081 gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc
     1141 gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt
     1201 gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct
     1261 tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg
     1321 gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa
     1381 gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt
     1441 attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc
     1501 ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc
     1561 agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat
     1621 tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac
     1681 tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt
     1741 cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg
     1801 aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg
     1861 ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc
     1921 aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg
     1981 agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa
     2041 tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc
     2101 atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg
     2161 actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga
     2221 aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga
     2281 aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag
     2341 caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg
     2401 atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt
     2461 caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag
     2521 taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc
     2581 cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct
     2641 tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg ataggaattg
     2701 gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca
     2761 aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc
     2821 aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa
     2881 cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg
     2941 agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct
     3001 ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc
     3061 ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg
     3121 gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac
     3181 tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag
     3241 actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg
     3301 aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa
     3361 tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa
     3421 cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct
     3481 ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta
     3541 tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa
     3601 tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc
     3661 agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta
     3721 tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg
     3781 aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat
     3841 ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag
     3901 ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc
     3961 ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag
     4021 aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg
     4081 tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc
     4141 cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg
     4201 tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac
     4261 gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata
     4321 aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca attacgttat
     4381 aaaacacatt ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg
     4441 gtgccaacca ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa
     4501 aagtccaagt acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact
     4561 aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac
     4621 agatttagaa atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag
     4681 tttcctaacc tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg
     4741 aacaagcaaa gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat
     4801 tatttcaagt aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc
     4861 ccaactttaa gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc
     4921 catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac
     4981 attggcagaa aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga
     5041 aatcaataag tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat
     5101 tatcggatat aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt
     5161 tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc
     5221 aatcattata catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt
     5281 tttggctgaa gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag
     5341 ccatgctgag gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca
     5401 aaaagcagca gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa
     5461 taatgatgtg attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc
     5521 cggcgctatc catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg
     5581 attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg
     5641 tttgagtggt gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg
     5701 tgccggtaac ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat
     5761 ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt
     5821 tgagatgcca attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa
     5881 agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta
     5941 aatagatgat caatagttaa tcgt
//
LOCUS       DACCPY010000124         5962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCPY010000124 DACCPY010000000
VERSION     DACCPY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.128"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IVO69_002533"
     CDS             complement(<1..126)
                     /locus_tag="IVO69_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7401273.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLY"
     gene            complement(575..835)
                     /locus_tag="IVO69_002534"
     CDS             complement(575..835)
                     /locus_tag="IVO69_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401274.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            910..1113
                     /locus_tag="IVO69_002535"
     CDS             910..1113
                     /locus_tag="IVO69_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7401275.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1254..1739)
                     /gene="ssb"
                     /locus_tag="IVO69_002536"
     CDS             complement(1254..1739)
                     /gene="ssb"
                     /locus_tag="IVO69_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7401276.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFVNCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKATTESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1742..2641)
                     /locus_tag="IVO69_002537"
     CDS             complement(1742..2641)
                     /locus_tag="IVO69_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7401277.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2654..3247)
                     /locus_tag="IVO69_002538"
     CDS             complement(2654..3247)
                     /locus_tag="IVO69_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7401278.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(3336..3500)
                     /locus_tag="IVO69_002539"
     CDS             complement(3336..3500)
                     /locus_tag="IVO69_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401279.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMETKTAAI
                     KEPAADSKRN"
     gene            complement(3519..3941)
                     /locus_tag="IVO69_002540"
     CDS             complement(3519..3941)
                     /locus_tag="IVO69_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401280.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEARERVE
                     GKIKSNQTKKYSPQLKKPVNESVYARVDPFDPTKILKR"
     gene            complement(3943..4239)
                     /locus_tag="IVO69_002541"
     CDS             complement(3943..4239)
                     /locus_tag="IVO69_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401281.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIENN
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            complement(4531..4743)
                     /locus_tag="IVO69_002542"
     CDS             complement(4531..4743)
                     /locus_tag="IVO69_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401282.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            complement(4764..5615)
                     /locus_tag="IVO69_002543"
     CDS             complement(4764..5615)
                     /locus_tag="IVO69_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401283.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIIHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            complement(5720..>5962)
                     /locus_tag="IVO69_002544"
     CDS             complement(5720..>5962)
                     /locus_tag="IVO69_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401284.1"
                     /translation="NDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGF
                     ISSVASRRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1645 a         1160 c          946 g         2211 t
ORIGIN      
        1 atacaaacga tagcttttga tacatttcgc tctttctcca caatcgcatt cgacagtatg
       61 tggtgcatga tagtttggga ataattcgct gaagggaaca ccccaataat caaatttctt
      121 tcgcatgatt gattctgcct cctcttagga aaggttttac ttcctttcgt tagttctttc
      181 tttattgatt tgtttgccag aatgaatcaa cgaaaagcga caaggacaag ggcgtgcagc
      241 gtgccattta cccttgtcct tgtcgctttt tggtgataaa aggcaacttg ttgccggtct
      301 ggcaaatcaa taaagagata gcgaacagga acgaaccttt tggtgagagg aagcttttcc
      361 tattttgtct tacaaagcaa gaccccttat cttagtgaac gtgcaacgtt cacttccttt
      421 cttctcgaac ggcggtggcg gttggagtga gagaaaggtt tttcaattga gaaggcgctt
      481 gtgccttcct aaacaggcct ttccctttct tcctgtttct atgtaaaaca aaaagagctg
      541 cagtcctaca ttaggattgc agctcttctt ttatttatat gcccattgat cgcgtaaaat
      601 gatcagggtt tctaatttgc attgattcaa tgactagttg atttcgttta ttgaagtgac
      661 ctgtcactga aatagtatac ttgccttctt ccaaaatcag caatttatcc gctatttcta
      721 tattggccac cacacaatta atcggttcat gatttgtacg taatgtaaat cgcacaagca
      781 atggctgtgt cttgattacc cgaattttat ccaataaacc agtataatgt tccatcgcga
      841 tcacctcgct aaaattatag caaaaacagc taaaattgag aatttttatt cgattttaga
      901 tatgctataa tgatcaagac agtaaagttt gtgacggtgg ctatccgatt gaaagtgagg
      961 tggtgcgtat gtgcgtactt ccaaaatctt acagaaagga gaagcctttt gcctgttgaa
     1021 acggcgctag gattaatgat tagtttcggt tctttgatcg caacaatcat ttttggaatc
     1081 ttagcagttg taaacaacaa tcaaaaaaaa taaccgtcag ctttagccgg tcgacgatta
     1141 tttttcaaaa ataatattaa gctggtcacc gtcttaaacg gtgctgttag gggggttgtt
     1201 agcgcaactc ctcttttcgt atccattata acaactttat tgatctttta caatcagaat
     1261 ggtaaatcat catcactaat atcgattgat tgtccgccaa atggattttg tgaagcgttc
     1321 aatccattgt tgttaggcgt agctacatcc cgttgttcaa aggtggtagg gcttggactg
     1381 ctggacgatt gatcagcgtg cgcacggctc tctgtcgttg ctttactttc taatagttga
     1441 aagttatcag ctacaacttc tgtcacatac acacgttgtc cttgttggtt atcgtaggtt
     1501 cgggtttgaa tccgtccgac aacgccaatc aagctgcctt ttttagctaa tgtcgccatc
     1561 gtttctgctg gtttgcgcca aatcacacag ttgacaaaat cggcttctcg attgccgttt
     1621 tgattcgtga aattacgatt aacggctaag gtaaaagtgg ccactgctgt gccacttgcg
     1681 gtataacgta aatctggatc tttggttaac cgtcctacta aagttacatt attaatcatc
     1741 tttatctctc catttcttga ttcatttgtt gtgatctttt aaactgcctt tttctttctt
     1801 gattttcaat tttgatcaca cttttcatta ggtgatcaat ttcgttgact gtatctgcaa
     1861 atgaaatgga attcgcaaac gtgttaactg agcgatattt ttcccactgc ttttccaaat
     1921 agtcgtttcc cctgatctta tccatttctc tttcataata aaaggataaa ggctttgtat
     1981 ctccacgttt tgtaaatgta tttcgaacac ttttgtataa ctctttcgta tcaatatttt
     2041 ctagtttcgg aatggtaaat aagtcgtagt aatcttttgc tctcgaatta tctgtgccaa
     2101 agctgaacgt agcacttagc ttttctgcaa taatttgttc tgttggataa gctggaatct
     2161 ccactttttt atcttcaaac attaattggt gatagtgtgt atctacttca ggataaatag
     2221 aatctcctgt tgtcacgtcc attttaaacg gtacacgtac tttctctaaa ttagccacca
     2281 atttaatttg aaatccagga taaaaatcgg cttctcttgt ttccttcatt ccttgaatag
     2341 agaaaacaat gccttctttt gtgggttttc tagttaaatc attaaaaatg ctcttcaatt
     2401 tttcttcagt taatttgcta tttctaaatg tcgtatcgat atcaattgtt gtccttctat
     2461 cgattccata ttttgatcct aataaaaagc cgcctttcaa aatgaagtca tttttgtact
     2521 ttgattcaga aatttgttca attagacgat ccatcataaa atgccgttgc actaactgtg
     2581 catttaatcc tgtactcttt gctaaatttc ttgtttttgc tgcaaattgt gtcggtgtca
     2641 tctttttcct ccatcaatat aagctttgca tgtggtattt catttctttt tctacatgta
     2701 gttttgtcat gtaatctctt aatttacttg gatctctcaa tgggtcttcc atgtactctt
     2761 ttaatgcttc taatttcatt tcatattcaa tttcatatct cggattccaa atatcacaaa
     2821 gtgtacgctc tttatcatac atagtaatag gatttccttg aaaggaagtt ccctctactt
     2881 ttcctagttc ataaacttct ttcttcacga aatgaaattg taactgctgc tcttttgtaa
     2941 ttgaacgatc aacgttatag ccatggggaa aagttaagtg aatgaatttc ggcatattcg
     3001 tagaaagctg gtaaatatct aaagtcgttt catgagagaa gattcccctg gaaaatcgtt
     3061 gctgaagcca aaataaatca tctattccat gaccatcctc tacataataa ccacgatcaa
     3121 ctttgatcaa ttttccttga ttcaccaatc tttgtacagt ggttgctgcg attcctaatt
     3181 ttctggcttc cttcatagat aaatttccat cgcgaatagc caacaattct ttcagtttct
     3241 cttgcattta cttccccccc ttttgttaca aatacactac attaaatata tcatgatagt
     3301 gtatttgtaa caaggttaat gtctatcgat ctaattcaat ttctttttga atccgctgct
     3361 ggttctttaa ttgctgcagt cttcgtttcc atttcttcca gcgctttttc tcttgtcata
     3421 taatcaatgt atgttgaaaa agatttttta ttgggcaagg taaattgaca agtgagaata
     3481 actgcaggcg atttattcat catgttcccc ttctactttt atctttttag aatcttcgtt
     3541 ggatcaaatg gatctactct ggcataaaca gattcattca caggtttctt caactgaggg
     3601 gaatattttt tggtttgatt agatttaatt tttccttcca cacgttctct tgcttcttgg
     3661 taaaccgttg aatttgtacc aattagtatt cctgtatctt tcaaatgaag atctgcaaac
     3721 tctgaaacca tttcggttaa aatagaaatt tctttactca ttccattcaa ctttgcttga
     3781 ttggaattta cttttttctc catttctgga tcataattca tacatagaaa ccgaacggct
     3841 tcactatagt ttttaacttc tgcatgagta gaaataatct catccaacac atcgatatgt
     3901 gtttggtgaa agtaaatgtg tttgtctata acctttgcca tatcaaagtt cacttcctct
     3961 attttgctcg atcaagattt ctaaaatacg attgttttgg ctaacatatt cttctaatat
     4021 ctttgtgtta tagctttgaa tttctgaaaa cttttctaat aactgatcaa acttttcttt
     4081 tttatattcc atcaaaggac gatcaaaatt attttctatt agtaatttta aataatcgtt
     4141 acgactccat ttattacccg tacggttact tagttcgtca catcttcgat caatttctct
     4201 tacgattttc tcatccacat ttcttattct tacttccata ttaattcctc caatttaaaa
     4261 aggatagcga aaaggagcct atcctttttg ctatcctact accctccttt tactatcctt
     4321 cactatcctt tcactatcct atttttaaat attttgttcc cacatttatt tttgagaaac
     4381 gtaataggca cagcctatgg gaacactttt tttaaaaagt gttcccacgt ttccttatgc
     4441 tctttacaaa taaaggcttt tagagacgtt ctggaatttt agattccttg aattgttccc
     4501 attcatcaat caattggcta taagtagcca ttaatttttt tcttgctctt gttgctttcg
     4561 aatcaacaaa aaatcagttt gattcgttaa ttctaaatat tctgttcggt attcaatgtt
     4621 catcgtaccc gttttactac atagtaactg attatgacgt tctttcactt tgaataactg
     4681 ctccttcttt aaggttttta actccatgcc aactataagc acaatcctgt atagaggaat
     4741 cattttcaaa cgattctcca taattattta tctttcattg gaagttctaa atcactactg
     4801 ttccaaaaaa tgtatggttt gttttgacga atattacttc catctaacca caattttccg
     4861 atgccatatc gtaggcttgc ttcgacttca taaaaattgt agatctcttt tcttcgatct
     4921 attgctttca tttcctggcc attttttaaa atagatctga tgcccttaac agtagattca
     4981 tggatactcg tgtgaatgat atccgtattg atcaattcgt catatcttcc aaaatcaaca
     5041 ttgtactcat aaccaagaag tgtcggaatc aggtcaattt caatttcttt ttcaattgaa
     5101 cgtttaataa gtttcgtgga tacatacccg acaaaaaaga gaataatacc cacttcaaac
     5161 aaatacatgt gtccatttac aaagtgaata aacgtcaata aaaaatcctt cactcctttt
     5221 gttgttcttc cagattgttc aatttgtttt attaaagtcg ctagaattgc atttacaaca
     5281 attaaatcga ataggctttt gaaaagtttt cctgctgtta atttttgcct ggtaaattgc
     5341 atgactaatt gagtaaaaat agacaatagc atgacaaaaa tcaacgtcat tagcactgta
     5401 aaaatgtctg atgctgcaat cctattggga tcatttatca agtaataagc cgttccaaat
     5461 actccaatcg agtaaacggt taaatcaaga ataagtcgtg ccccgtaact acaaagtttt
     5521 cctaatggcc ataattgcaa tacccaaaat ttctttcgtt tgctatttct tttcagagga
     5581 agccaggtgt gttcaatcca gtcgtccgat ttcatttcga tttcttccct aaattctttc
     5641 ctttaacgtt ttcctttgct actttagcag cgataggttg ctcaatgaac caatttttta
     5701 agtaatatta tttgtcaaat taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa
     5761 cttctaatgg tgtttggtaa tttagtgatt ttctagggat atggtttctt ctggacgcaa
     5821 cggatgagat gaatccttga ttgacttggt tgaagtccat ttcttttggt agtccatctt
     5881 ttcggaggag tccgtttgaa tgttcattta atccccgttg ggaaggcgtt cctgggtctg
     5941 cgaaataaat atcaatatca tt
//
LOCUS       DACCPY010000125         5931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCPY010000125 DACCPY010000000
VERSION     DACCPY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.129"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IVO69_002545"
     CDS             complement(<1..450)
                     /locus_tag="IVO69_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7401285.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVO69_002546"
     CDS             674..1465
                     /locus_tag="IVO69_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7401286.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVO69_002547"
     CDS             1431..2384
                     /locus_tag="IVO69_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7401287.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVO69_002548"
     CDS             complement(2782..3756)
                     /locus_tag="IVO69_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7401288.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVO69_002549"
     CDS             3871..4305
                     /locus_tag="IVO69_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7401289.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVO69_002550"
     CDS             complement(4430..5416)
                     /locus_tag="IVO69_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7401290.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5931
                     /locus_tag="IVO69_002551"
     CDS             5844..>5931
                     /locus_tag="IVO69_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7401291.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPS"
BASE COUNT         1864 a         1121 c         1015 g         1931 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca a
//
LOCUS       DACCPY010000126         5912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCPY010000126 DACCPY010000000
VERSION     DACCPY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.130"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IVO69_002552"
     CDS             complement(368..1486)
                     /locus_tag="IVO69_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401292.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IVO69_002553"
     CDS             1957..3264
                     /locus_tag="IVO69_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7401293.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IVO69_002554"
     CDS             3383..4582
                     /locus_tag="IVO69_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7401294.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IVO69_002555"
     CDS             complement(4609..5877)
                     /locus_tag="IVO69_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7401295.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1816 a         1252 c         1058 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aa
//
LOCUS       DACCPY010000127         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCPY010000127 DACCPY010000000
VERSION     DACCPY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.132"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(72..542)
                     /locus_tag="IVO69_002556"
     CDS             complement(72..542)
                     /locus_tag="IVO69_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7401296.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IVO69_002557"
     CDS             872..2512
                     /locus_tag="IVO69_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7401297.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IVO69_002558"
     CDS             2666..3988
                     /locus_tag="IVO69_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7401298.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IVO69_002559"
     CDS             complement(4056..4490)
                     /locus_tag="IVO69_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401299.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IVO69_002560"
     CDS             complement(4555..5391)
                     /locus_tag="IVO69_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7401300.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1128 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgtt
//
LOCUS       DACCPY010000128         5617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCPY010000128 DACCPY010000000
VERSION     DACCPY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.133"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(118..432)
                     /locus_tag="IVO69_002561"
     CDS             complement(118..432)
                     /locus_tag="IVO69_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7401301.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IVO69_002562"
     CDS             complement(446..706)
                     /locus_tag="IVO69_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7401302.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IVO69_002563"
     CDS             876..1190
                     /locus_tag="IVO69_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7401303.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IVO69_002564"
     CDS             1192..2847
                     /locus_tag="IVO69_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7401304.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IVO69_002565"
     CDS             2930..5065
                     /locus_tag="IVO69_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7401305.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..5605
                     /locus_tag="IVO69_002566"
     CDS             5240..5605
                     /locus_tag="IVO69_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401306.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
BASE COUNT         1909 a          970 c         1140 g         1598 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatacgccgt
     5581 ttgtataatt aagcgagaca aataaaaaag ccattta
//
LOCUS       DACCPY010000129         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCPY010000129 DACCPY010000000
VERSION     DACCPY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.134"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..1107
                     /locus_tag="IVO69_002567"
     CDS             133..1107
                     /locus_tag="IVO69_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7401307.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IVO69_002568"
     CDS             1089..1550
                     /locus_tag="IVO69_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7401308.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IVO69_002569"
     CDS             1600..2064
                     /locus_tag="IVO69_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7401309.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IVO69_002570"
     CDS             2078..2827
                     /locus_tag="IVO69_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7401310.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IVO69_002571"
     CDS             2827..3657
                     /locus_tag="IVO69_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7401311.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IVO69_002572"
     CDS             3657..4304
                     /locus_tag="IVO69_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7401312.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IVO69_002573"
     CDS             complement(4402..5178)
                     /locus_tag="IVO69_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7401313.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IVO69_002574"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IVO69_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACCPY010000130         5435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCPY010000130 DACCPY010000000
VERSION     DACCPY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.135"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            250..1524
                     /locus_tag="IVO69_002575"
     CDS             250..1524
                     /locus_tag="IVO69_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401314.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1578..3005
                     /locus_tag="IVO69_002576"
     CDS             1578..3005
                     /locus_tag="IVO69_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7401315.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3018..3890
                     /locus_tag="IVO69_002577"
     CDS             3018..3890
                     /locus_tag="IVO69_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7401316.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3985..4980
                     /locus_tag="IVO69_002578"
     CDS             3985..4980
                     /locus_tag="IVO69_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7401317.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1866 a          754 c         1052 g         1763 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgttataaa aaatagtgcc
       61 attaaaaaag aaaagcgaca caccaataaa agtcatatcc tgtgacatta tatggcatta
      121 tccaaataga ataaccggtt gacatgtaag cggatttctt ttataatttt aggagtacaa
      181 gtccttttct aaaaatgtta accggttatc atgaaagggg atacaaatat atcgttggag
      241 gataaaaata tgtcaaaagt taacgtattt aaaaatcaat cttatttaca aagttcagct
      301 acattattac tattttttgc ttcttggggt gtttggtggt cattttttca actttggcta
      361 acatctgaat caaatggttt agggttatct ggcagtgctg taggaacagt attctcggca
      421 aattcgttag ttaccttaat tttgatgttt atttatggaa cattacaaga taaattgtat
      481 attaaacgaa atttattaat ttttgcttct gtattagcga cacttgttgg accatttttt
      541 atatggatat atgggccatt gctagataac aattttaatt taggcattat tatgggagcg
      601 ctatttttgt cagctggata tttagcttct gtaggagttt ttgaagctgt gtcagaaagg
      661 tttagtcgtt tatttggctt tgaatatgga caagcaaggg cgtggggatc atttggttat
      721 gccttggtag cgcttttggc aggattttta tttgtaaaaa atcctcattt aaacttttgg
      781 gcgggatctt tctttggttc tttactattg ttaaatttat tattttggaa ccctaaagtt
      841 gaacgggaag caaatcaaaa ttttaatcaa gaacaagctg aatcaaatag tattccttct
      901 ttaaaagaaa tgtttgatct aatgaaactg cctcaattat ggacgataat catctttatt
      961 gtttttacat ggacatttta tacggtattc gatcaacaaa tgtttccggg attttatact
     1021 ggtttgtttt caacatcagc taatggtgaa aaaatatatg ggacattgaa tgctattcaa
     1081 gtattttgtg aagcgttaat gatgggaatt gttccaatca ttatgagaaa attaggggtt
     1141 cgaaatactt tgttattagg tgtaaccatt atgtgtgtac gaattggatt gtgcgggttt
     1201 gcctcgacac cattatctgt ttcatgcata aaaatgttgc atgctttaga agtaccatta
     1261 tttacattac caatgtttcg ctattttaca cttcattttg atacaaagct atcagcaacc
     1321 ctctatatga taggatttca gatagctgct caaattgggc aagtgatttt atcaacacca
     1381 ttgggaatat taagagacaa cgttggctat caaccaacat ttaaaattat ttctcttatt
     1441 gtattactag caggcatata tgcattcttt attcttaaac aagatgatag agatgttcaa
     1501 ggggatccat ttattcgagg ataatggaaa ccaacagaat aaaaaacaat tttatctaga
     1561 aagtgagcga atatattatg acaaaaagag ctatagaatt aacaaataca cgttatcgtt
     1621 tgggatatca tgtgtctgca ccatcaggat ggattaatga tccaaatgga ttttgctatt
     1681 ttgatgggta ttatcatgtg ttttatcaac atcatccata tagtgctgaa tggggaccaa
     1741 tgcactgggc acatgctaga agtaaggatt tagttcattg ggaatcttta ccacttgctt
     1801 tgactcctgg agatcaagaa gatgaaggtg gttgtttttc aggaagcgct attgaaaaaa
     1861 atggtgtgtt gtatctgttc tatacaggac atcactatta tggagacaat gatccagatc
     1921 atttttggca aaatcaaaac atggcgtata gtacggatgg cattcacttt actaaatatg
     1981 aaaaaaatcc tgtaatagca aaagcaccag aagataacac acatcatttc agagatccaa
     2041 aagtatggaa acacgatgaa aaatattaca tggttttggg tagtcaagaa aaagatggat
     2101 taggtcgtgc tattgtttat cagtcgttag atttattaaa ttgggattac attggaccaa
     2161 tttctaaagc gaaggaagta cttacagaag gatttatgtg ggaatgccca gacttttttg
     2221 aattaaatgg aaaatctatt cttttattat cccctcaggg aattgaagca aatggaaaag
     2281 attatttaaa tctatatgaa acaggctact ttatcggtga ttatgattat gagacagcaa
     2341 aattcattag aggaaatttt tatgaattag ataagggaca cgatttctat gcaacacaaa
     2401 caactttatc tccagatggt agacgtattg ttatggcctg gatggatatg tgggaaagcc
     2461 ctatgcctga aaaagtagac ggatgggcag gagcattaac gcttcctcga gaattagagt
     2521 taattggaga tcgtttgtat atgaaacctg tgagagaatt agaacaactt cgagttggca
     2581 gcggcgttga aacgacagtt aatttggcag gagaaataat aatcgctcaa gatgcttcat
     2641 tcaaagaagc tctaattgat gtactattga ctaaatcaaa aaatcaagaa gtcaaatttt
     2701 cattaaaaac aaaagatgaa gagttaatca atatttttta ttctaaggat aaaaatgaat
     2761 gtaccttaac tcgtaacggc aaaaatgatc caaggtatgg aaccattcag atgtgtgata
     2821 gattatcact tcgagtgttt attgataaaa gttcggcaga gatttttatt aatgatggcg
     2881 agcttgtttt tacagagcgt tattatacag aaaatcaacc aagtatgcat ttgagtgtta
     2941 gtgaagaagc ttttgtctcg agtacaattt atcatctaaa aaaagatgca atttcttacg
     3001 agtaaggagg ttttatgatg ttaattggta gcatagaagc aggtgggact aaatttgtat
     3061 gtgccgtggg agacgaggat taccgagtac aagaccaaat aatttgtcca acgaccaccc
     3121 ccagggaaac attgaaaaaa actgttgact attttaaaca gtttgatata aatgcattag
     3181 gtattgcatc atttggtcca atagaaattc gaaaaaattc accaaaatca ggatatatta
     3241 cgtctacacc aaaggtggga tggcgaaatg ttaatgtgtt aggttattta caagatgagc
     3301 tgaatgttcc aatgtcttgg acaacggatg tgaacggatc agcttatgga gagtacatca
     3361 tgtccacttt atcgaatgaa aaaataaatt cattagttta ttacaccgtt ggtactggcg
     3421 ttggtggtgg agctgttgtt gacggaaaac tattaggatc gtttggtcat tcagaaatgg
     3481 gccatacgtt tgtaaaacgc catcctgatg atattgattt taaaggaatt tgtccttatc
     3541 atggtgattg tttagaaggc ttagttgcgg gaccaacgtt tgaagcacgt tttggaaaaa
     3601 agggtgaaga cgtttcttta acggatccta tttgggatgt aatggcctat tatctagcac
     3661 aagctgtgat tcaaacaaca ttgattatac gaccggataa aatagtattt ggtggaagcg
     3721 tagtaaatga aaaattgtta gaaaaaattc gtgttaatgt taataaatta ttggatgatt
     3781 acgtggaaat accaccgtta agtaaatata ttacgcgtcc tttaattaaa aataatggat
     3841 cggcaacact aggaaacttt gctttgggct tgaatcaatt acaagaataa aacatagaaa
     3901 aaatatcgat tatcaggtat agtagttcta tagagttctt tttttcgata gttaattatt
     3961 gcattattta ggagagacaa gagaatgcgc ccaaaattag aagatgttgc caaaagagca
     4021 aatgtatcaa aaacaactgt ttcacgtgta ttaaacaatc gtggctattt aagtcaaaaa
     4081 acgattgata atgtttataa ggctatagaa gaactcaatt atcaaccgaa cgttgtagcg
     4141 cgtcaattat ttcaaaagaa aactaacatt gtcgggttac tatttcctac agtagccaat
     4201 ccttttttta gtgaactcgt tgaggcattg gaaaaaaagt tatatgaaat tggttataag
     4261 gtattaatag gcaattcgat gaataataaa gaaaaggaaa caaattattt aaatcagtta
     4321 ttgtctgatc aagtagatgg tttaattgtt ggtacacata accaaggaat tcaggaatat
     4381 aaatatcaga atttaccgat tgttgcaatt gatcgagtga tgaatgaaga tattccagtt
     4441 gttgaatccg ataactacaa tggtggaaaa cttgcaacca agttattaat tgcacaaggt
     4501 gcaaaaaata ttattcatac aaacggtcca attgacttac agactccggc taatcgtcgg
     4561 agactagcct atgaagatac tatgaaagca tatcagttaa ttccacgaac tgtgacatta
     4621 gatttcaata tttcttatgt aaagaaaaaa cagatttttt tccaaatgtt tgaggattat
     4681 cctaaaatag atggtatttt tgcttcaaat gacatcgatg cggccctaat attacaagta
     4741 gcaaaagaaa aggggttaaa tgtaccagct gatttattag tagtcggata tgatggtact
     4801 ttaatgactc ggagtatatt accagatctg acaacagtaa tacaaccaat taatgatata
     4861 gcagacacag ctgttgctat tttgatgaaa cgaataaata aagaagaaac aaaaaaggaa
     4921 tatattttac ctgtcacatt atggatgggg aagacatcag taaagaaaaa gaacaattaa
     4981 atttataaag actatcaatg ataatttcat tattaattcg tgatataaaa aattgttaaa
     5041 tctcttataa tagactatga tacaaaatca tgcttttaat ctgtgtagat gttgccaaat
     5101 atttgaaaga catagaactt gtcagaaaaa ccttaacaat tcctcaatgg gcggataagc
     5161 taggcaaaag ggctggcata aatttttcag tccttctaac agaatcaatt gcagataaag
     5221 cagataacat tcttcgttct ggaagaaata attagaactc aaaagggtca gaaacttaaa
     5281 agtttttgat ccctttttta ttcctcattt catagctttt ttagtagctc aattaattag
     5341 gtcagaaagt tactttttta aaaggacacc agagtatacc ctgttgggct cttggtgttc
     5401 tattgaatgt aaatgagtgt catttttata tttaa
//
LOCUS       DACCPY010000131         5281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCPY010000131 DACCPY010000000
VERSION     DACCPY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.136"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(42..1190)
                     /gene="nagA"
                     /locus_tag="IVO69_002579"
     CDS             complement(42..1190)
                     /gene="nagA"
                     /locus_tag="IVO69_002579"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7401318.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1266..2102)
                     /locus_tag="IVO69_002580"
     CDS             complement(1266..2102)
                     /locus_tag="IVO69_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401319.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2286..3503)
                     /locus_tag="IVO69_002581"
     CDS             complement(2286..3503)
                     /locus_tag="IVO69_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7401320.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3886..3969
                     /locus_tag="IVO69_002582"
     tRNA            3886..3969
                     /locus_tag="IVO69_002582"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3920..3922,aa:Leu,seq:gag)
     gene            complement(4209..5207)
                     /locus_tag="IVO69_002583"
     CDS             complement(4209..5207)
                     /locus_tag="IVO69_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401321.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1637 a         1076 c          837 g         1731 t
ORIGIN      
        1 caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc
       61 acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca
      121 tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc
      181 agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta
      241 attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt
      301 ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg
      361 gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt
      421 cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct
      481 aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg
      541 aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta
      601 gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct
      661 tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct
      721 tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct
      781 ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt
      841 gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac
      901 gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact
      961 ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta
     1021 tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca
     1081 ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt
     1141 ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct
     1201 ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac
     1261 accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct
     1321 gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg
     1381 aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac
     1441 gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa
     1501 aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa
     1561 tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc
     1621 agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct
     1681 tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg
     1741 agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa
     1801 gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt
     1861 tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc
     1921 gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta
     1981 ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc
     2041 attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc
     2101 ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt
     2161 cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc
     2221 cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt
     2281 taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc
     2341 aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat
     2401 ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca
     2461 tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt
     2521 ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt
     2581 tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg
     2641 gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc
     2701 agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact
     2761 cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca
     2821 ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt
     2881 tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat
     2941 tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc
     3001 cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg
     3061 caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag
     3121 tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg
     3181 atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt
     3241 ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag
     3301 tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca
     3361 aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc
     3421 atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg
     3481 ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt
     3541 tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac
     3601 ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc
     3661 atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag
     3721 cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc
     3781 cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca tttttgagct
     3841 tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa
     3901 ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca
     3961 tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt
     4021 tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta
     4081 aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg
     4141 ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt
     4201 tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat
     4261 ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc
     4321 attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc
     4381 catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata
     4441 actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat
     4501 agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt
     4561 ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat cattgccctt
     4621 ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg
     4681 tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat
     4741 gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac
     4801 ggctttccaa atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat
     4861 tcaggaaacc atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa
     4921 gagaaaatta attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt
     4981 ccattgacaa tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact
     5041 tgttcttgat tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt
     5101 atttcttctc ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact
     5161 ggccactgat agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt
     5221 attaaacagt atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca
     5281 t
//
LOCUS       DACCPY010000132         4516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCPY010000132 DACCPY010000000
VERSION     DACCPY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.138"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IVO69_002584"
     CDS             complement(<1..95)
                     /locus_tag="IVO69_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7401322.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIY"
     gene            complement(140..904)
                     /locus_tag="IVO69_002585"
     CDS             complement(140..904)
                     /locus_tag="IVO69_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7401323.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(932..1630)
                     /locus_tag="IVO69_002586"
     CDS             complement(932..1630)
                     /locus_tag="IVO69_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7401324.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1642..2421)
                     /locus_tag="IVO69_002587"
     CDS             complement(1642..2421)
                     /locus_tag="IVO69_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7401325.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(2437..3381)
                     /locus_tag="IVO69_002588"
     CDS             complement(2437..3381)
                     /locus_tag="IVO69_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7401326.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3427..4206)
                     /locus_tag="IVO69_002589"
     CDS             complement(3427..4206)
                     /locus_tag="IVO69_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401327.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1176 a          933 c          801 g         1606 t
ORIGIN      
        1 aaatagatat atcgtttttc aactttttct ttatccaacc aaacttgatt aggatgtact
       61 tcactaatac gagataataa atcttcactt tccatacttt ccctctctcc tcatttcctt
      121 tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc tcttcataga
      181 ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta
      241 ctttttcctt accaaagtcc ttagcaatct gctcatagca ctcttttaag taaaaggtac
      301 gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt ttgaccattg
      361 ttttggctgt tttctgaatg tctttgccaa atacaccaac ataacttggc gcagtaattt
      421 gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttct cgaaaatgcg
      481 cgttgcgttc tggatggacg atcactggtg tctttcctag ttctaacaac tcaaaaaaaa
      541 gccattctgc atataacggc acttccagtg tcggaaattc gatcagaaga tatgtatcat
      601 ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac
      661 gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat tcggcttgta
      721 acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca ttattgtggt
      781 gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc attgcaatac
      841 ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa tgtagatcga
      901 tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa ccagcatctt
      961 tgctatgttc tgctccgttg tatacaacac ctaaaactcg tgcttgtacc atctctagca
     1021 gttcttttgc tttattcaat gattctttac gtgatgtatt ttcacgtacg accaataaag
     1081 tgccatccgt ttttgaagac atgatctgtg catctgttac ggctactact ggtggcatat
     1141 cgaatatcac cacgtcaaat aactgtcttg cttcgtctag gatctgatcc attcttggcg
     1201 aacttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc gacaagttct
     1261 cgactggcgt tcgttggatt acatctgcta cacttcctga tgaactaagt gctgtactta
     1321 atcccctggc attgttcagc tggaatgtct tatagacgac gggtttacgt aaatctgcat
     1381 cgactagtag aactcgttga ccggatttag caaaaacgac tgcaatgttt gctgcagtgg
     1441 tggattttcc ttctccagga cctgatgaag tcacgacaat cgttttgatt tgctgaccag
     1501 ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg attggtgaag
     1561 atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt tgtttctgtg
     1621 ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg gggctgtttt
     1681 atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac cagcgttcaa
     1741 ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg catccgtcac
     1801 atatttttca tctttcactg tccgatcaag taattcgaat aagaatgcca gacctacgcc
     1861 taccatcatt cctaatacta agccaatcac tagattcaat ttattatttg gcgaaacagg
     1921 tgtcgtattg gcaactgcac ctgaaataat cgagatttta tcgacattca tcacgtcttt
     1981 tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag tcactgcatt
     2041 cgtgcttatt gcctggatcg agaacatttg tgaattttgt gactgattga cggagatcgc
     2101 atctttgatt tgtccagcag tcattttcac attgtcttct gtttctaaac gatctttgac
     2161 ttcatttagt actaaatcac tgacgatcat atctttatac gtattgatca tttgcaggtt
     2221 cgtattgaca tcattgacat ttgctgtgtc tgattgtggc agtgtcacga tcaattgtgc
     2281 ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga tggccaagcc
     2341 agcaaacatt gagatgatga ttgttaccaa gtgtttgcgt aatgtttcaa aaatttcttc
     2401 caagttgatt gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg
     2461 atcttttaat tcttttttta cacgatcaag ttcttgatca ctgacttttt gataggagat
     2521 attatcttgc ataaagcctt ctccttgcag ctgatcttgc ttgatcgtac caaatgcctc
     2581 ccgataatct aaagcgattt ttttcatttc atcaaagctc atgtctgttt tcatattcga
     2641 ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttaaactca atactttttt
     2701 tgctacacct tctacgatct ttctttgacg ctcttgtcta ccatagtcac catttggatc
     2761 ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtga ttttaccgat
     2821 tgtaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga taccaccaac
     2881 tgcatcaaca agttccttta gccctttcat attgatagag acatagtgat cgattgggat
     2941 gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg
     3001 gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatcac gaggaatgct
     3061 gacgatcgtt gtctgtttgt cttcaggact gactatggca accatcatcg tatctgatct
     3121 tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa cagagaaaga
     3181 ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt ccactgattc
     3241 gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta ctcctacaat
     3301 tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt tccctcttga
     3361 ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga atcaacgttt
     3421 taactgtcaa taacttatat tgctatagcc catttgctga gcaacagttt gtgcttttgt
     3481 tgcgtatgct gcgcttattt tttcattccg gatctgctct atttgactga gcgccgtcaa
     3541 atattgatcg taagtagcgg tggcagtcac ttgaccatcc ttcaacatcg tatcgaataa
     3601 ctgatctagt tgcgtgactt gcgcgatctg agtatcagcg aatcctaaat attgcataac
     3661 aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac gagcgacatc
     3721 tgcacttgtc accttttctt tgatgatgac atcgtctaca gcttgctgcc agtttgatac
     3781 atttttagta aacaaatcgt tgacagctgt ctggatcttc agttttgatt ctgcactatc
     3841 taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt taggcaattc
     3901 tgattctttg atgtcgtaat catctgctga aagtttgaca taggatagct tatttgacaa
     3961 acttgtgatt gtttcttctg ttatttcagg gtttagaaaa attttttctt ttgtactata
     4021 cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg tgatggcaga
     4081 ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt aaccaaacaa
     4141 acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga cgaatggctt
     4201 ttccatagat tcactcctaa gtattgatga tagataaatt aagatataat tcacttacat
     4261 aaaaatgaca gctttttatt ttaaaaaaca taattaaact aatatattca cagaaatatt
     4321 tgtaaacaag ttttccctat ataatcatac cacaaactgt tttcttgact agggcaattt
     4381 atgatttaaa agtaaataaa acattaataa gataacaatt aatcgatttc atttttattt
     4441 ccttaatttt cataggtgtt tttaaaagtt acacaaaaaa taaaataaca aaaaaattaa
     4501 cattgaatta ataata
//
LOCUS       DACCPY010000133         4400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCPY010000133 DACCPY010000000
VERSION     DACCPY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.139"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(253..783)
                     /locus_tag="IVO69_002590"
     CDS             complement(253..783)
                     /locus_tag="IVO69_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7401328.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            888..1439
                     /locus_tag="IVO69_002591"
     CDS             888..1439
                     /locus_tag="IVO69_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7401329.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1592..2065)
                     /locus_tag="IVO69_002592"
     CDS             complement(1592..2065)
                     /locus_tag="IVO69_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7401330.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2203..3675
                     /locus_tag="IVO69_002593"
     CDS             2203..3675
                     /locus_tag="IVO69_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7401331.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3672..4382
                     /locus_tag="IVO69_002594"
     CDS             3672..4382
                     /locus_tag="IVO69_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7401332.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1382 a          799 c          848 g         1371 t
ORIGIN      
        1 ataaacggtg ttcaaacggc tgcttctcgg taataagccc actagcctag aaaatatgaa
       61 aagctatttt ctaactagcg ttcttattac tcaaagcata acgctgattt cacaacctct
      121 tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaacatga
      181 ggaactattt ttccgtttct ttctctaata ctcaggtcag aacgctgatt tcacaacctc
      241 tctttcacaa ccttatttta ctcgtttata attttcatcg aagtattttc cgctacggat
      301 atcatctggc attccatcgt atggttcttc gctaataaca tcttcattgt ctatacctgc
      361 atcacccata taagtgatcg tagcaaattc aggaacaagt aattttggat aagtttcata
      421 tttttcacga gctgcttcca tttctgcgat atgttcgttt tggaaagtaa cagtgatttc
      481 tggatgttct tcaacccatt tagggaaaaa gatcattccg tgaagtttct tttcatttgg
      541 cataaagtcc aatgccacgt ctgtccctgt tggctctgtc caagtgattt tgaatacgcc
      601 gtccgtcaat ttaacgatat cagcttcttg gtcttttacc caacggcctt tgaccattcc
      661 gccatggata cggtaatcaa cagtatggtc atttttggca taccattcat attcccaacc
      721 attgtcatac gtatagataa agtgagttcc aataaaatct tccaattctt taaattcttt
      781 catgtttaag catctcctta taaattaaaa catgtaataa actacatgtt ttcgattact
      841 catttatgat agaatggatt taaagatttg tcaaggaggg aaaactgatg gcaagaaaaa
      901 atacacttcg atatattttg ttagggttat taagtaagaa gaagatgaca ggatatgaat
      961 tgaatcaatc gttcaaaaat gaaataggag aattttggca agcaaaacat agtcagattt
     1021 atcctgaact tgcaaaaatg gaagagcaag gaatcattca gcatcaagta gagatcacag
     1081 gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga agggaaggaa caattggcag
     1141 agtggattca tacacccacc aatgaactac cggttaatcg agacgaattt gttctgaaat
     1201 tgtatttcgt caaagacgtg aatgatccgg cattagtaga aattattcag cagcagcgtg
     1261 accttcatga agaaaaacga cttcatctat tagagcgaaa aaaaacgatc tttcctactg
     1321 aaaaagaaaa acatgaaaat tacggacact acctgatttt agcgcatgcg atcaatcggg
     1381 aaacagaata tgcaaattgg ctaacagatg tattagaaga aagtaaccga aaaaaataaa
     1441 aaaatatccg aacagtgatg gagaattcct ttctatatga aagaaacgca gatcaccgtt
     1501 cggatattct tatgttcaaa caggcctttt ttcaagtatt tatgtagcat ctacttatta
     1561 aaaagcttgg tacttaggag attagtggcg tttagagttc ttttaacaac tgttcgtagt
     1621 cagctttttc atcgacagga aggagcttgt aagttaattc gtctgtctcg ttgtcatgaa
     1681 tgatgtacat cttagcattc tcttctttga agttaccgat ttgtaaaatc gtatagcgtt
     1741 cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac tttacggaca tggtgcattt
     1801 tcttttggcg gatttctgcc ttttctgcag cttttgacaa agtaaatccg tcatctaaat
     1861 attttttgat cagttctact tttatgactg taggtaaacg atattttcta ggacaattcg
     1921 catcagcttc gatcgaacga atgaatccct tttgttccca ataccttaat tgacgtggag
     1981 acacacctac gatatcactt aattcactga tgccaacaac caattgatca ttttccaaca
     2041 aacgtttaag ggaagcccca accatttttg tcactccttt tgtcctcttg cttcccttaa
     2101 ttatatccgt attaacttat gtgtcaagta ggttgacaaa tagcgaattt aggactaaag
     2161 tattctagta gtttcagaaa aaattcagaa agaaggagaa atatgagtaa caaccaaccc
     2221 gtagatattt acggaaaacc atataatcgg acacttttag ttgttgtctt attaattggt
     2281 acattttgta ccgttttgaa tcagacctta ttgacgacag cttttcctac attgatgaaa
     2341 gctttcgata tttcagcgtc tgatgtgcaa tggttaacga ctggtttctt gctagtcaac
     2401 gggatcatga tcccaataac agcatggctg atcaataaat tcagttcaag aaatttatat
     2461 ctttctgcca tgacgatctt tttgatcgga acgattactt gctttacagc acctaatttt
     2521 agtacattac taatcggtcg tttgatccaa gcttgcggtg tgggagtatc gatgccatta
     2581 ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa gaggatcagc aatggggatg
     2641 tcagggatcg ttattggact tgctcctgct ttaggcccta cattatctgg ttatatcatt
     2701 gataactacc attggcgtga cttgtttggt atggtactgc caatcgttgt tcttgtcttg
     2761 gttttagcat tcttcctaat gaaaagtgtt attccattgt caaatcctag cattgatatt
     2821 ttatccgcta ttttatcaac gattggattc ggtagtttac tttacggatt ttctagtgta
     2881 ggagatgacg gctggggaag cttgaaagta attggtttct tagttgttgg tgtgattttc
     2941 atcgccttgt tctcatggcg tcaattacat ttggaacatc cattcttgga acttcgtgta
     3001 ttcaaatcat ctacattcac gattgcagct gttttagctg gtgtaacaaa tatggcgatg
     3061 gtaggggcag aaatggtcgt accattgtat attcaaaata ttcgtggaga atcagcattc
     3121 cattctggct tgatgttgtt gccgggtgca ttgatcatgg gggctatgat gcctgtgacg
     3181 ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca tcacagggat gttcattttg
     3241 acagcggcaa ctgcaccatt tgctttcttg acaaaaacga ctccagtttt atatatcgtg
     3301 atcctttatg cgatccgtat gtttgggatc tccatggtca tgatgccggt aacgacttct
     3361 ggtatgaatg cattgccaaa taacttgatc agtcatggga cagcggtaaa taatacattc
     3421 cgtcaaatcg caagctcgat tggtacagct attttgatca gtgtgttgac gaatgtgaca
     3481 aaagcaaata tcccaagcaa atctgtattg gataatacgc cattagctta taaagatcag
     3541 gcaatcaatg ccacactgtc aggttaccat gccgcgttta tcgtagcgat catctttggg
     3601 attcttggct ttattattgc ctttttattg aagaaaaaag aacctgttct aatgaaagcg
     3661 ggggataatg catgattatt ggagttttga ttgtcagctt gcttgctttt gctttaacga
     3721 acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt gattttcgga ctaatcttcg
     3781 tcgcttcact aagtttgatc gtcgctaatt tgtccaatca ctttggaatg gaaaaagtaa
     3841 cggaaacaaa aacagaaaaa attgtttcca gtgctgattc ccaaggagca gatatgcttt
     3901 tgtataaagc tttagggaat ggaaaagaaa aagtgtatct ttaccgaaca aatgaaaaac
     3961 aagaaaaacc aaaagcaact ggtacggata acgaaacaaa taaagtagaa aagacagacg
     4021 gcgatgctga aaaagtgacg aaaactactt attgggaata caaaaacgac atgtataaat
     4081 tctggttcaa tattgctgat aacaatcacg aatatgacag ccgagtgaat acattcaaaa
     4141 tcccagagac atgggtagaa ttaagtacgg accaagcagc caagttagct gaattagtga
     4201 agaaacagca aagtacgatg gaaagtgaag caaaagctta tgttcaagac ggtatggtaa
     4261 aagcaatgac tgaaaatcca aaaatgagca aagcagagca agaacaacgt acgaaagcct
     4321 tagctgctga attccaacaa caagcattcg caaaattagt gaaagaagca aaaggcgaat
     4381 aatattttga acaccatgta
//
LOCUS       DACCPY010000134         4052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCPY010000134 DACCPY010000000
VERSION     DACCPY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.142"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IVO69_002595"
     CDS             5..1150
                     /locus_tag="IVO69_002595"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7401333.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLATSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVO69_002596"
     CDS             1321..1779
                     /locus_tag="IVO69_002596"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7401334.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVO69_002597"
     CDS             1938..2408
                     /locus_tag="IVO69_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7401335.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            2541..3968
                     /locus_tag="IVO69_002598"
     CDS             2541..3968
                     /locus_tag="IVO69_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP7401336.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT         1353 a          746 c          747 g         1206 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagcgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct cagactgtag
     2461 acaaactcct aaattgggag tgttgtttgc agtttttttg ttttatactc attgtataaa
     2521 ctattctggg agtgattcta atgatgagaa aacaatcgat tgaaggtaga aatcaatttg
     2581 ctatgttaac aattgatgat cttgtaccta aagatcactt agttcgaaag attgatgctg
     2641 cgattcagtt tgatttcatt tatcccattg tagaatctac gtattcgacc catggtcgtc
     2701 ccagtattga tccagtggtc ttaattaaat tagtatttat ccaatattta tttggtatcc
     2761 gttcgatgag acagactatt aaagacgttg aaacaaatgt tgcttatcgt tggtttttag
     2821 gttattcatt cgaggatcct atcccgcatt tctctacttt tggtaaaaac tatgttcgaa
     2881 gattcagaga aacaacttta tttgaagaca ttttctctca tattctagaa caagctgtaa
     2941 aagctggctt cgttacggaa gacaaccttt atattgattc tactcatatt aaggcaaatg
     3001 ccaataaaca taagttcaca aaagaaatga cctacggtca agctaaagct tatcaagatg
     3061 aattagaaga tgaaataaat aaagaacgta ttgctgtagg aaaacgtcct tttacatggg
     3121 acacagaaag tgagttgagt cttcaaaaaa taagtcattc agatccagaa agtggttact
     3181 atgttaaagg tgaacgagaa aaacaatttg cttattccgc gcatacttcc tgtgaagata
     3241 atggttttat cttatctaca ttaatcacac caggaaatgt acatgacagc caagtcgcaa
     3301 tcgatctggt caaacagtcg aaaagagctt ttccaaatat aaagcgtgtt gtcgctgatg
     3361 caggatataa gacgcctaaa tttgttcatt ttctgtcaca cttgaaactc tacccgattc
     3421 ttccatatac tgtacctcat ggagtaaaag gaatgtttcg taaaaaagac tttgtttatg
     3481 atgcctattt tgactgctat ttatgtccca ataatcaacc gttgttattt tcaactatca
     3541 ctcgtgatgg ttatcgaacc tataaatcaa atccaaaatt ctgtaggagt tgtccattac
     3601 ttaaaaactg tactttgagt acaaaacatc aaaaaatagt aactcgacat gtttgggcgg
     3661 atcttatgga tgaacttgaa caccaacgcc atacggcgtt gaatagagaa atttataaaa
     3721 agagaaaaca aaccattgaa cgaatatttg cagatgcaaa agaaaagcat ggtatgcgtt
     3781 ggacgaaata tcgaggtctc gaaaaagtcg ccacgcacac gatgcttacg tttgctgcca
     3841 tgaatttaaa aaaactagcc acatggctat ggaaagcaaa aaagcctatg gctttttatt
     3901 taaaatttct acaaagacta acaaaaagga gttggcaatt aagccactcc tttttgtcta
     3961 cagtctgaga tgaactattt ctagttcatc tttttgcctt tgcgtggcga cgtcctactc
     4021 tcacaagggg caacccctca ctacaatcgg cg
//
LOCUS       DACCPY010000135         4039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCPY010000135 DACCPY010000000
VERSION     DACCPY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.143"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IVO69_002599"
     CDS             179..775
                     /locus_tag="IVO69_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7401337.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IVO69_002600"
     CDS             899..2698
                     /locus_tag="IVO69_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7401338.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IVO69_002601"
     CDS             complement(2976..3188)
                     /locus_tag="IVO69_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401339.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1187 a          688 c          712 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaata
//
LOCUS       DACCPY010000136         3973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCPY010000136 DACCPY010000000
VERSION     DACCPY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.144"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IVO69_002602"
     CDS             591..1280
                     /locus_tag="IVO69_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7401340.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVO69_002603"
     CDS             1267..2475
                     /locus_tag="IVO69_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7401341.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..>3973
                     /gene="serS"
                     /locus_tag="IVO69_002604"
     CDS             2769..>3973
                     /gene="serS"
                     /locus_tag="IVO69_002604"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7401342.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQN"
BASE COUNT         1290 a          687 c          787 g         1209 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tga
//
LOCUS       DACCPY010000137         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCPY010000137 DACCPY010000000
VERSION     DACCPY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.145"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IVO69_002605"
     CDS             <1..156
                     /locus_tag="IVO69_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP7401343.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVO69_002606"
     CDS             175..1452
                     /locus_tag="IVO69_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7401344.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVO69_002607"
     CDS             1462..2118
                     /locus_tag="IVO69_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7401345.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVO69_002608"
     CDS             2118..3494
                     /locus_tag="IVO69_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7401346.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IVO69_002609"
     CDS             3624..>3956
                     /locus_tag="IVO69_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7401347.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          424 c          693 g         1245 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACCPY010000138         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCPY010000138 DACCPY010000000
VERSION     DACCPY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.146"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(150..425)
                     /locus_tag="IVO69_002610"
     CDS             complement(150..425)
                     /locus_tag="IVO69_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7401348.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(397..1350)
                     /locus_tag="IVO69_002611"
     CDS             complement(397..1350)
                     /locus_tag="IVO69_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7401349.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1962..3455
                     /locus_tag="IVO69_002612"
     CDS             1962..3455
                     /locus_tag="IVO69_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7401350.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3590..3886
                     /locus_tag="IVO69_002613"
     CDS             3590..3886
                     /locus_tag="IVO69_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7401351.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1323 a          645 c          712 g         1276 t
ORIGIN      
        1 ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc ccaaacaata
       61 cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat actaaatata
      121 tttactatat ctagtatact tactatattt taatcttttg atcgtctatt atatataatc
      181 cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac tatttcatac
      241 ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg aggatctact
      301 tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac attaatatca
      361 tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt tggtaataat
      421 gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg agtcagcata
      481 tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt caattctttc
      541 ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta cataatcatt
      601 taattggata ccattctcac catacatttc taaccgatcc gagcctttaa caatgctttt
      661 aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt gttgagagga
      721 acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag actcttcaaa
      781 attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt ccattgcgta
      841 ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg cacgaaccac
      901 attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc tcaattcttc
      961 taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt cggacaagta
     1021 ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg gacttacttg
     1081 agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc gatcaaagct
     1141 agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt cataatcaat
     1201 tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat cattgtctgt
     1261 agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta gtttatctga
     1321 tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat actaaatata
     1381 tttagtatac ttcatatact taatatattc gatatatcta atatactcca aattgataag
     1441 aattcagact ttataaggaa tatagtggat atactaaata tatttgctat attcagtata
     1501 tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga
     1561 aaagaggtaa acttaattct atagaaacca cagataattc actatttaca aagtagcaaa
     1621 agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa ataattgatt
     1681 ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa
     1741 gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc ttttccgatt
     1801 gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaaagtttat ttagtgtact
     1861 taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag attaaagcta
     1921 aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc gaaatttatt
     1981 cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc atgaaacctg
     2041 caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc aagcaattaa
     2101 tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag aacgaaaaga
     2161 aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat gattatcgaa
     2221 cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt gtagttgata
     2281 tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat caagtgggct
     2341 acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt gtactaaaga
     2401 caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt
     2461 caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta ggatgtaacc
     2521 acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa atgaactatc
     2581 agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat caaaaaacaa
     2641 aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag
     2701 atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga gaaggacgtc
     2761 ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca ggttatgcat
     2821 tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa ccgctatctg
     2881 aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa gcagcaagca
     2941 gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa aataatgatc
     3001 tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa agaaaatata
     3061 gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa agtcttgcta
     3121 gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg atgcccaagc
     3181 gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc tacaaggtaa
     3241 cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc gcgagggtta
     3301 ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag gtatttggac
     3361 tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg aaccctctta
     3421 aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca aattgcccta
     3481 tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg
     3541 gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa
     3601 aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt
     3661 ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt gggttaccag
     3721 ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct tagcttctgg
     3781 ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt atattgagta
     3841 taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt ttttatttac
     3901 gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac tcaaat
//
LOCUS       DACCPY010000139         3944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCPY010000139 DACCPY010000000
VERSION     DACCPY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.147"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1211)
                     /locus_tag="IVO69_002614"
     CDS             complement(<1..1211)
                     /locus_tag="IVO69_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7401352.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..2796)
                     /locus_tag="IVO69_002615"
     CDS             complement(1201..2796)
                     /locus_tag="IVO69_002615"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7401353.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            2921..3706
                     /locus_tag="IVO69_002616"
     CDS             2921..3706
                     /locus_tag="IVO69_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP7401354.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1233 a          762 c          640 g         1309 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
     2581 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     2641 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     2701 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     2761 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
     2821 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
     2881 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
     2941 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
     3001 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
     3061 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
     3121 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
     3181 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
     3241 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
     3301 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
     3361 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
     3421 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
     3481 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     3541 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     3601 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     3661 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     3721 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     3781 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     3841 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     3901 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DACCPY010000140         3941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCPY010000140 DACCPY010000000
VERSION     DACCPY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.148"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IVO69_002617"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IVO69_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IVO69_002618"
     CDS             complement(545..739)
                     /locus_tag="IVO69_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401355.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IVO69_002619"
     CDS             2091..3131
                     /locus_tag="IVO69_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7401356.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1445 a          545 c          584 g         1367 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt g
//
LOCUS       DACCPY010000141         3909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCPY010000141 DACCPY010000000
VERSION     DACCPY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.149"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            108..3149
                     /locus_tag="IVO69_002620"
     CDS             108..3149
                     /locus_tag="IVO69_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401357.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3339..>3909
                     /locus_tag="IVO69_002621"
     CDS             3339..>3909
                     /locus_tag="IVO69_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP7401358.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1343 a          622 c          827 g         1117 t
ORIGIN      
        1 taataaatca aatgattgat aataagatag ctgaattgat ggaacttgag agttataaaa
       61 aagctctcat ctacgaatac gtaaccggta aaaaagaagc ctaggaggtg attcatttgg
      121 tagagaacaa agaaattcgt tttgagcagg atattgagac ttcactgact acgtatggca
      181 aatggaagaa agaaacattc aatgaatcaa actttgatcc cataattggt ttggattgtc
      241 aaaaattgct aggctttgtc cagaagactc aaccaaaaca gtgggcacgt caccaacgaa
      301 actataacaa tgatggagaa gtcaagttcc ttcaacgctt taatcaagag gtagaaaccc
      361 acggcatgtt acatgtattg cgcaatggga tcaaagaccg cggtgtgcgc tttaaagtag
      421 cttacttcaa acctgcttca gagctgaatg atgaaacgat ggaaaagtat gaagcaaata
      481 gttttacctg tactcgccag tttgcttaca gtcctaataa ccataaaacc attgatatgg
      541 ttttgtcgtt aaatggtatt ccgctcgttg ctttagaatt gaaaaaccag tacactggtc
      601 aaacagtaga aaatgccaaa ctccagtttc aaaaagatcg tgatccaaaa gaattggtat
      661 ttcgttttaa tcgccgcttc ttagtctatt ttgcggtgga ccatacagat gtttatatga
      721 cgacaaagtt ggacggtaaa aaaacgtact tcttgccatt caatcaaggt tccaatgggc
      781 cgggaagagt tggtggagca ggcaatccgt ccaataaaga tggctaccca actgcctact
      841 tgtgggaaaa ggtgcttact agagacaatt tgatggatat tatccaacgt tttatgaatc
      901 tagagattaa aaaagaaaag caaggcgaca gagaagtcac aaagaaaacg ttgatttttc
      961 ctcgttatca tcagttagat gtggttcgaa aattggttgc agatgtaaaa agccatggta
     1021 gtggagaaaa ttatttgatc cagcatagtg ctggttctgg aaaatctaat agtattgcat
     1081 ggctggcata ccatttagcc tctattcatg atcaactgaa ctacccaatt ttttcatcaa
     1141 ttattattgt gactgatcga acagtgttgg atcgccagtt gcaaaatact atcatgaact
     1201 ttgatcatca gacaggtttg gtagaaacga ttgacgatcg caaatcctct caagatttaa
     1261 aagaagccat taatgatggg cgtaagatca ttattaccac tttgcaaaag ttcccagtga
     1321 tctatcagga agtagatcaa gtggaaggtc gtaattttgc tgtgattgtg gatgaggcac
     1381 attcttcaca aactgggaac agtgcccaga agctaaaagc cgctctttcg gacaaatcta
     1441 aatccttgga agagtattat gaactggaag gcgaagttga cgatgtatta ccagatgctg
     1501 aagatgaact agttcaacaa ttggtagcac aaggtaaaca tcaaaattta agtttctttg
     1561 cttttaccgc tacaccgaaa gaaaagacat tggaaatgtt tggtacgaaa caaccagatg
     1621 ggcatttcga accctttcat gtttactcga tgcgccaggc gattgaggaa ggctttatct
     1681 tagatgtgct gaagaactat atgacctata gtacttccta caaaattgcc cgtgaaattg
     1741 aagataaccc agaattacca aagagtgaag caacccgagc gattgttcgt ttccaatcat
     1801 tgcatccttg ggcagttact caaaagacgg ctgtgatggt tgagcaattc cgagcggtta
     1861 ctcgtacagc tattggtggg caggcaaaag caatggtcgt taccgcatca cgcttacacg
     1921 ctgttcgcta tatgaaagaa tttaaaaaat atatagcgaa caaaggttat aatgacttgg
     1981 atgtgttggt tgctttttct ggttcggtag tagatggaga gactgaatat acagaaacga
     2041 agatgaatgt cacaaaatct ggtgagaatg ttcgggagaa tcaattaaag gaagctttcc
     2101 atacagataa ttttaatatc ttgattgttg ctgaaaaata tcaaacgggt tttgatgaac
     2161 cgttgctgca taccatgttt gtcgataaga agttaaaggg tgttaaagct gttcagactt
     2221 tatcacgact caatcgaaca atgcctggga agaaggatac ctttattctt gattttgtaa
     2281 atgatgttga agatattgaa gctgctttcc aaccctttta tgaacaaaca aatttgagag
     2341 aagaaatcaa tgttaacttg atttatgata ctcaaagcaa gctacgaaaa tttaatgttt
     2401 acaatcaagg tgatattgaa cgtgtcatga aattggtgaa acaagcccaa aagcaacaga
     2461 atgagcgctt gcttggacga atttctagca tgtttaagcc gattatggat cgctatgagg
     2521 aaatgaacaa ggataatcag taccagtttc gggtgactct acgtaacttt ggcaagtggt
     2581 atgattatat ttcacaactt gatcggttat tcgatatgga actattggag gaacgaattt
     2641 ttactggata cttgctgaag tttctaccta aagaaacacg agaaaatatt gatatttctg
     2701 acaaagtaaa gttggaatat tataagttac gtcaggactt ttccggtgaa atccatctag
     2761 tgaataaaga aggcgagcta gtaaatccaa agtcaattga tgcaggaatc aaaccgccgg
     2821 atgaacgaga ttcactagaa gaaatcattc ataaaatcaa tgaacgattt ccaaatgatt
     2881 ttgacgaagg agaccgggtg ttagtagaga ttttgtatcg gtcatttgcc gataacccag
     2941 atttgaaggt tgtcagcatg gctaagaata acgatgctca gatgtttgaa aaatcgctgt
     3001 tccctgatgt atttaaagat aaggtgatgg agcagtacga gaagaacaat gcagcttttg
     3061 agaaaatgtt tgggcatgat gataagtact ttgatctagt atatacatta gttgctagag
     3121 atttatacaa gttattacgg acgaaataaa atttataaat aaggattaga caatacaatt
     3181 tctctcaaaa ataaaggaga agttgtattg tttgctttta agaaagccct gaaaaatttg
     3241 ttacgtacta aattgtctga actctgtcat atcagctatg tcagaataaa ggaaatagat
     3301 ttatttttat aaacagtgtc gtctcggaga tttaaactat gtcaacgaca attgaagtga
     3361 acaaacaaag cgtgaagcag tttcttgaaa cagggaaaat caaaaaattc gttattccag
     3421 aatatcaacg tccttatgcc tggactgacg agcaaaacca agtactattt gatgatttag
     3481 cggagtacac agcaaacaat aacgagagta cttacttttt aggaagcatt gtggcttatg
     3541 aaaatgatca taacgagcaa gaaatcattg atggacagca aaggataact acgctatttt
     3601 tatttttgag agctatctat gctaaattag aaaatagttg tgaaaaggaa gctttattct
     3661 tgaaaagcca aattgaaccc gcgttatggg aacaagatga tttaacaggt gaggttaaac
     3721 cagataaaat tctgattatg tctcgagtaa tgggggatga ggggaatgaa gaatttgcta
     3781 gtattctagt aagtggtgaa gcagatgtta aatcaaaaag taattattcg aagaactata
     3841 ttttaattca acatctatta aatgagtatg caacaaacga accactatca ttttatcgtt
     3901 ttatcagta
//
LOCUS       DACCPY010000142         3863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCPY010000142 DACCPY010000000
VERSION     DACCPY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.150"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(247..468)
                     /locus_tag="IVO69_002622"
     CDS             complement(247..468)
                     /locus_tag="IVO69_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401359.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="IVO69_002623"
     CDS             complement(566..811)
                     /locus_tag="IVO69_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401360.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="IVO69_002624"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="IVO69_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..3036)
                     /locus_tag="IVO69_002625"
     CDS             complement(2035..3036)
                     /locus_tag="IVO69_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7401361.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3195..3461)
                     /locus_tag="IVO69_002626"
     CDS             complement(3195..3461)
                     /locus_tag="IVO69_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401362.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3451..3807)
                     /locus_tag="IVO69_002627"
     CDS             complement(3451..3807)
                     /locus_tag="IVO69_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7401363.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2341 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2401 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
     2461 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
     2521 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
     2581 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
     2641 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
     2701 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
     2761 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     2821 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     2881 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     2941 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     3001 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     3061 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     3121 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     3181 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     3241 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     3301 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     3361 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     3421 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     3481 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     3541 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     3601 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     3661 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     3721 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     3781 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     3841 agtatataac ataaaaattc gaa
//
LOCUS       DACCPY010000143         3720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCPY010000143 DACCPY010000000
VERSION     DACCPY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.151"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            484..780
                     /locus_tag="IVO69_002628"
     CDS             484..780
                     /locus_tag="IVO69_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7401364.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            781..1197
                     /locus_tag="IVO69_002629"
     CDS             781..1197
                     /locus_tag="IVO69_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7401365.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1217..2761
                     /locus_tag="IVO69_002630"
     CDS             1217..2761
                     /locus_tag="IVO69_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401366.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2904..3128
                     /locus_tag="IVO69_002631"
     CDS             2904..3128
                     /locus_tag="IVO69_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401367.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3143..>3720
                     /locus_tag="IVO69_002632"
     CDS             3143..>3720
                     /locus_tag="IVO69_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7401368.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDK"
BASE COUNT         1318 a          620 c          807 g          975 t
ORIGIN      
        1 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
       61 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
      121 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
      181 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
      241 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
      301 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
      361 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
      421 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
      481 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
      541 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
      601 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
      661 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
      721 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
      781 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
      841 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
      901 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
      961 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     1021 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     1081 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     1141 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     1201 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     1261 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     1321 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     1381 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     1441 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     1501 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     1561 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     1621 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     1681 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     1741 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     1801 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     1861 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     1921 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     1981 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     2041 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     2101 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     2161 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     2221 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     2281 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     2341 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     2401 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     2461 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     2521 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     2581 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     2641 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     2701 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     2761 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     2821 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     2881 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     2941 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     3001 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     3061 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     3121 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     3181 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     3241 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     3301 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
     3361 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
     3421 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
     3481 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
     3541 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     3601 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     3661 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
//
LOCUS       DACCPY010000144         3458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCPY010000144 DACCPY010000000
VERSION     DACCPY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.153"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..375)
                     /locus_tag="IVO69_002633"
     CDS             complement(<1..375)
                     /locus_tag="IVO69_002633"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7401369.1"
                     /translation="MKNISLELKNIEVNFGNKELVAIKYLTVYENDRIGIVGRNGQGK
                     TTLLNLIQGKLSPNSGEVNRFVEFNYFKQMEETNNLYEFDDLNAELISRFTIPRNTIE
                     VLSGGEENKLRLARVLSNYKMGL"
     gene            complement(984..1703)
                     /gene="catA"
                     /locus_tag="IVO69_002634"
                     /pseudo
     CDS             complement(984..1703)
                     /gene="catA"
                     /locus_tag="IVO69_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
     gene            1912..2871
                     /locus_tag="IVO69_002635"
     CDS             1912..2871
                     /locus_tag="IVO69_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401370.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKVAKIAEFLKRSRTPIYNVIHF
                     LKEGHTALDYYQQYKENKKRCGRQKIILPKEQQTYIKEKVNQGWTPDVIIGRKEMIIS
                     CSMRTLYRQFKEKVFDEATLPMKGKRKPNGHQERRGKQAFKRNIVERKTDYPDFEEEF
                     GHIEGDTIVGAHHKSAIITLVERLSKVIITLKPDGRRASDIEATMDHWFNAIPRHLFK
                     SITFDCGKEFSNWKALSNQHDVAIYFADPGTPSQRGLNEHSNGLLRKDGLPKAMDFNL
                     VDQSFISSVADKRNNIPRKILNYQTPLEVFMSYMNKDILSSLI"
     gene            3189..>3458
                     /locus_tag="IVO69_002636"
     CDS             3189..>3458
                     /locus_tag="IVO69_002636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401371.1"
                     /translation="MKNKLVSYAIMTVNNRYNRDYYSLFIPFVAETIRLKEFKYITPQ
                     IIKKEIQEKYDLEIPTAVINQIFNKLDKEKLIERKYYADQKKYLYH"
BASE COUNT         1241 a          570 c          536 g         1111 t
ORIGIN      
        1 taatcccatc ttataatttg atagcactct agctaaacgt aatttatttt cttcacctcc
       61 gcttagtact tcgatcgtat ttctcggaat agtgaatcga ctaataagtt cagcgttaag
      121 atcatcaaat tcgtataaat tattggtttc ttccatctgt ttaaagtaat taaactccac
      181 aaatcggtta acttcccctg aatttggtga taactttcct tggataagat tcagtagcgt
      241 tgttttccct tgtccgtttc ttccgacaat gccaattcga tcattctcat atacagttaa
      301 atatttaatg gctactagtt ctttgtttcc aaaatttact tctatatttt ttaattctaa
      361 agatatattt ttcattattt tgcactcctt aaattaggcc gtgcaatcaa cagatttttg
      421 ggtacacaaa aaagagatag atttctacct cttttgacaa tttccatatc tatattttct
      481 tagataaata gaatttgaaa aatagacaga ttgctcccct gtttctgcca atataacagg
      541 accttttgaa taatagtatt taattattat ttcaaaaggt taaaccacaa tcttatagat
      601 gctgtcattt tccaaattct cctttgtaca tcctatttgt atactagata ctgctcaaat
      661 gaacacgtat ctttctacca acgtctcata aagcttaaaa catttttttc aaatagtcaa
      721 gttatgctca cctattatag gtcaagataa cttataatag gtgatatgaa aaaaaatact
      781 cttgctcctt tttcgccatt caaggtaact tcaatataac atgaaattca catggcgttt
      841 tttagttagc ggtggctaac ttcattatat aatccaccgt aaaaaatatt attagttaca
      901 tttgtcatta tagtcttaac ttaagagttg tggcattgct tattacatat gtaataagca
      961 atgcgttttg tgaagtagta cacttaaatt aacgggcagg ttagtgacat tagaaaactg
     1021 actgtaaaaa gtacagtcgg cattgtctca tattataaaa gccagtcatt aggcctatct
     1081 gacaattcct gaatagagtt cataaacaat cctgcatgat aaccatcaca aacagaatga
     1141 tgtacctgta aagatagcgg taaatatatt gaattacctt tattaatgaa ttttcctgct
     1201 gtaataatgg gtagaaggta attactatta ttattgacat ttaagttaaa cccagtaaat
     1261 gaagtccatg gaataataga aagagaaaaa gcattttcag gtataggtgt tttgggaaac
     1321 aatttccccg aaccattata tttctctaca tcagaaaggt ataaatcata aaactctttg
     1381 aagtcattct ttacaggagt ccaaatacca gagaatgttt tagatacact atcaaaaatt
     1441 gtataaagtg gctctaactt atcccaataa cctaactctc cgtcgctatt gtaaccagtt
     1501 ctaaaagctg tatttgagtt tatcaccctt gtcactaaga aaataaatgc agggtaaaat
     1561 ttatatcctt cttgttttat gtttcggtat aaaacactaa tatcaatttc tgtggttata
     1621 ctaaaagtcg tttgttggtt caaataatga ttaaatatct cttttctctt ccaattgtct
     1681 aaatcaattt tattaaagtt catttgatat gcctcctaaa tttttatcta aagtgaattt
     1741 aggaggctga cttgtctgct ttcttcatta gaatcaatcc ttttttaaaa gtcagtatta
     1801 ttgtaacata aatatacgcc gattgtaaaa ttaagctaga caaataaaaa agccattcgt
     1861 ggtacactca aaatgtattt ccgaccaaag aaaacatagg agtgatcacg aatgacctac
     1921 acccatctta ccacggatga attagtgatg atagagtctt attatcacca aaatattaaa
     1981 gtcgctaaaa ttgctgagtt cttgaaacga tctcgaacac ccatttataa tgtcattcat
     2041 ttcttgaaag aaggccatac ggccctcgat tactaccaac aatataaaga aaataagaaa
     2101 cggtgtggca gacagaaaat catcttacca aaagaacaac agacctatat caaggaaaaa
     2161 gtaaatcaag gctggactcc tgatgtcatt attggacgaa aagaaatgat catcagctgt
     2221 tctatgcgga ctctttaccg ccagtttaaa gaaaaagtct tcgatgaagc tacacttcct
     2281 atgaaaggga aaagaaaacc caatggtcat caagaacgca gaggaaaaca agccttcaaa
     2341 cggaatattg ttgaaagaaa gaccgattat cctgacttcg aagaagagtt cggtcatatc
     2401 gaaggcgaca ctattgtggg ggctcatcac aagagcgcca tcattacctt ggtagaacgt
     2461 ctttctaagg ttattattac tttgaagccc gatgggcgga gagccagcga tattgaagct
     2521 accatggatc attggtttaa tgccattcca aggcatttat ttaaatcgat cacttttgac
     2581 tgcggaaaag aattctctaa ctggaaagct ttaagtaacc aacacgacgt tgccatctat
     2641 tttgctgacc caggaacacc ttcgcaacgt ggtctgaatg aacattccaa cggtctcttg
     2701 cgaaaagacg gattgcctaa ggcgatggat ttcaatctgg tggaccaaag tttcatttct
     2761 tctgttgcag acaaacgaaa caacatccct agaaaaatat tgaattacca aacgcccttg
     2821 gaagtattta tgagttacat gaataaagat attttgtcta gcttaatttg acaaatcaaa
     2881 aaattaaaaa agggataaaa aatatgaaga tcgaatgact tatcatgtaa tcatgaataa
     2941 tttgaagtaa gaggagctaa tcaagaagat atctgaagaa aaagatgtgg caagagatac
     3001 tgtgtataag attaagaaat aatttgagat aaaggaataa aaagaaatta ttacttttat
     3061 tgtaaccaag gaataaatta aataattaaa tttataatat taaaataact atcgtataga
     3121 attcataagg aataatcact ataatatgag taagagtatt ataatcattt tatggaggga
     3181 ttgtcttttt gaagaataaa ttggtttcat atgcaataat gactgtaaat aatcgatata
     3241 acagagatta ttattctctt tttataccat ttgttgcaga aacaattaga ttaaaagaat
     3301 ttaaatacat aacaccgcag ataataaaaa aagaaattca agaaaaatat gacttagaaa
     3361 ttccaactgc tgtaataaat caaatattta ataaactcga caaagaaaaa cttattgaaa
     3421 gaaaatatta tgcggatcag aagaaatact tatatcat
//
LOCUS       DACCPY010000145         3316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCPY010000145 DACCPY010000000
VERSION     DACCPY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.154"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1155)
                     /locus_tag="IVO69_002637"
     CDS             complement(<1..1155)
                     /locus_tag="IVO69_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401372.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..>3316)
                     /locus_tag="IVO69_002638"
     CDS             complement(1158..>3316)
                     /locus_tag="IVO69_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7401373.1"
                     /translation="SEDGKLLHLYYGENLPDNDYSHLIEMHHRPMTTYRNENDLLYSL
                     EHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVEEGKPKLEGLPATYVDNN
                     DESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNKGKDIQIIENISSLSLDL
                     PDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDENQ
                     GEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYSS
                     KGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDVG
                     IELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEMV
                     NKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDYI
                     KWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGRF
                     DPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKT
                     RGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQ
                     TAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRA
                     GLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1075 a          624 c          482 g         1135 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgata
//
LOCUS       DACCPY010000146         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCPY010000146 DACCPY010000000
VERSION     DACCPY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.156"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(191..376)
                     /locus_tag="IVO69_002639"
     CDS             complement(191..376)
                     /locus_tag="IVO69_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401374.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1226..1567)
                     /locus_tag="IVO69_002640"
     CDS             complement(1226..1567)
                     /locus_tag="IVO69_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7401375.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1567..1797)
                     /locus_tag="IVO69_002641"
     CDS             complement(1567..1797)
                     /locus_tag="IVO69_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7401376.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2093..2431
                     /locus_tag="IVO69_002642"
     CDS             2093..2431
                     /locus_tag="IVO69_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401377.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2636..3211)
                     /locus_tag="IVO69_002643"
     CDS             complement(2636..3211)
                     /locus_tag="IVO69_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7401378.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT         1047 a          578 c          484 g         1112 t
ORIGIN      
        1 aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa tttttaaact
       61 atcattttta tcctcaacta catacatatc tgttgcaact tcactttctg tctcatccag
      121 ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagcc
      181 aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca
      241 aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg
      301 attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt
      361 tagaattttt actcattcat aaacacttcc ttaaatagct tttattatag aaggtattgt
      421 aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt atactggaat
      481 gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag
      541 taattttatt aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac
      601 ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc
      661 acattttcga gcattacaag tattggtgtc gttagaacaa ctaaatagaa actagcggta
      721 gatttttcag aatctatgct aatttctgta cggatgttcc agtgatggca aatttctctt
      781 ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa
      841 ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa
      901 aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc
      961 tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg
     1021 aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc
     1081 gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatcccat cgaccacact
     1141 agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa
     1201 atattataga gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag
     1261 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
     1321 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
     1381 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
     1441 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
     1501 ctgagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
     1561 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
     1621 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
     1681 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
     1741 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
     1801 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
     1861 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
     1921 ttgtagccag ttcatttttt ctttaaataa aaaaataatc caagaaacat tgaacctatg
     1981 gatccaaaag ctacccaaat actatagcta actccccgct accttcaatc ttgcagataa
     2041 aacgctttgg aaaaaaatcg agaaaatcgc aaagacgaca ggccatccta tcatgcctga
     2101 ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg gaaagttcac
     2161 gaaactagca ggtgaagaag tttcaactgt tcggattaaa gaatgcccga ttcaaataga
     2221 aacattggtt caacgaattt caactagaca agactttgct attgtagaac gtgagatcgt
     2281 agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg tggataaatg
     2341 gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt taactttgaa
     2401 ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta taaataaaaa
     2461 agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt cgactgtgac
     2521 cctatagatg cagtacatgc agcttatgag catttcttct gttctctttg tttttgggat
     2581 ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga ttatgttata
     2641 ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca aaagaaatat
     2701 ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg tcatggattg
     2761 gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt ttttcatcct
     2821 gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa aacataaatt
     2881 tgtctttttc ttcattccac tcataaaagc cactcattgg gaaaacacaa cgattttgca
     2941 aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttgatcatca
     3001 attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt
     3061 cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt
     3121 ctgctgcctg ctccggttgt ttcttctggg cttctcgata ataattctga aggagctctg
     3181 actctagatc aaaaaaataa cgaccacaca tcctaatccc t
//
LOCUS       DACCPY010000147         3007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCPY010000147 DACCPY010000000
VERSION     DACCPY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.158"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(276..692)
                     /locus_tag="IVO69_002644"
     CDS             complement(276..692)
                     /locus_tag="IVO69_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401379.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IVO69_002645"
     CDS             complement(695..979)
                     /locus_tag="IVO69_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401380.1"
                     /translation="MDILVRNIDPKFVATIDKLCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IVO69_002646"
     CDS             complement(1679..1774)
                     /locus_tag="IVO69_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7401381.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IVO69_002647"
     CDS             2427..>3007
                     /locus_tag="IVO69_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7401382.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          958 a          477 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caaagtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACCPY010000148         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCPY010000148 DACCPY010000000
VERSION     DACCPY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.159"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IVO69_002648"
     CDS             complement(106..1053)
                     /locus_tag="IVO69_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7401383.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IVO69_002649"
     CDS             1299..1763
                     /locus_tag="IVO69_002649"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7401384.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IVO69_002650"
     CDS             complement(1825..2868)
                     /locus_tag="IVO69_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7401385.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACCPY010000149         2892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCPY010000149 DACCPY010000000
VERSION     DACCPY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.160"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            433..600
                     /locus_tag="IVO69_002651"
     CDS             433..600
                     /locus_tag="IVO69_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401386.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            674..1900
                     /locus_tag="IVO69_002652"
     CDS             674..1900
                     /locus_tag="IVO69_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7401387.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2058..2675)
                     /locus_tag="IVO69_002653"
     CDS             complement(2058..2675)
                     /locus_tag="IVO69_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401388.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT         1001 a          476 c          435 g          980 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaatactt tagataaatg gattcgtata tataattttc ccgtaataaa aatagacggt
      541 ataattcgct ttgatgtaga agatatcaaa ggctttatgg atacgttcaa aaagatttga
      601 attttacttt tgactgcata tactaaaagt agcgaaatat actgccttac ttttttatta
      661 aggagtgagg aagttgccta cgaagctttc aaatggaaaa tataaaacta atttgagata
      721 tccaaaaaaa tttaaagaaa ttacaggtat cagttcagaa aaattccaaa aaacatttcc
      781 aactaggcaa ttggctatta aagccgaaaa tgaaatgaaa aagaaaatcg caaaagtctt
      841 aagagaggag aatgcaaatt cattagagct caaaggtcaa ataaaattca aggagtttta
      901 taaatctaaa tggcttccta gatatgagtt agggcaaact actagatccc ataaagctcc
      961 aagcgatata accatcatta atacaaagaa tttatttcga ttgcatattc tgcctatatt
     1021 tggtgagtat gccatgagct atcttaatgc aaataccgaa ataatatcag atgaactcac
     1081 aaaaaagtcg aaggaatatg caaacataaa aatcattaaa tgctatgttc gttccatgtt
     1141 tgatattgca gaggttctaa attatattga atttaatcgt acaacaaaaa taattcaaag
     1201 tattactgca cctaagaaag ttgctctgga agaaaaaaga atacgagaag gaaaacaagc
     1261 tttatcttcg aaagaactca ctgattggtt agacgctgta agagatgatt tcaatgatca
     1321 attactttcc tttcacgatt acactctttt tatgcttact ttatatcttg gggatagaaa
     1381 gagtgagacc tatgctttgc aatggaaata cattgatttt gaaaaccaaa cagtccgtct
     1441 taaatatgcc ttggataaac acacaaaaaa gaaatttaca aaaggtcgaa aagatactat
     1501 tatccaagtt ccggaaattg taatgacact attgcaagaa tggaaatcag tgcaagctga
     1561 acaactattg aagttgaaaa ttaaacagac acctgaccaa tatcttttta gttactccaa
     1621 accttctggt gaagtaaatt gtccagtcca tactgattac ttaaattatc gaattaattc
     1681 tataaaaaaa agacatcccc atttggttca tttaactcct cataaacttc gacatactta
     1741 cgcaacttta gctagacaag gtggggctga tatgaatcaa atttctaacg ctttgacaca
     1801 ttcaggtatt tcaactacaa aaatttatgt gaatacacct gatttagttg ataaagctgt
     1861 gttcgaagcg tttcagcgag gattacaaaa atctaattaa tgtgttctag aatgtgttct
     1921 aaatataaaa aaaagacact ttccaaataa ttggaaagcg tcttgaacgt tgatatataa
     1981 aggatattaa cgttttgaga attgagatgc tttacgagct ttcttaagac ctggtttctt
     2041 gcaaaattat attagcctca actgaaagat ttattttttt gctccctaac ttcattagta
     2101 ataccatgtt tcattcgata tcttctaaac gttctacgat taatccctgt tagctcttca
     2161 acttctttat ctgtagatcc agtcaaaaac aaattaaatg ctaattgaag tcgcggatca
     2221 ttctcacgat attttagcgt acctcccttg tatttccctt ttgctttagc gagtgcaatt
     2281 ccttgttgtt gcgtttcgcg aatataccga cgttcttctt cggcctggta cttgtatagt
     2341 tcgataatta agttattaag taatcgacgt aaattatcat cagtgatacc tgttaacgct
     2401 ggcaaattca aaacttccag tgtagctttc ttcagctgaa ttaaattcat cgtttctgtt
     2461 agctctttat tatttcgtcc aagtcgtttt aattctgtca caacgacaat atcaccttca
     2521 cgtatgaatt tcatcatttc ttgaaatcct tctcgattgg tatctttccc acttgcttta
     2581 tcactaaaaa ttttatcaca ctctgataat aattctaatt gtcggtctaa attttgatct
     2641 aagctgctta ctcgagcata cccaattttc gccataataa aactccttta aaattctgaa
     2701 atccggatca tttttaaatt ctgttgccca actctgttgt catttttcta gataaatggc
     2761 ataaattttc agcgctaagt gacaaaagca actaattttt catgatagat ttttaaatat
     2821 ctcaatctat ctccaaaatt agttgctttt gtagctatag ttgactatga atatgacaaa
     2881 taattagaaa aa
//
LOCUS       DACCPY010000150         2841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCPY010000150 DACCPY010000000
VERSION     DACCPY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.161"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            43..507
                     /locus_tag="IVO69_002654"
     CDS             43..507
                     /locus_tag="IVO69_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401389.1"
                     /translation="MELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQI
                     DDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHR
                     FIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            566..1006
                     /locus_tag="IVO69_002655"
     CDS             566..1006
                     /locus_tag="IVO69_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401390.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1018..1686
                     /locus_tag="IVO69_002656"
     CDS             1018..1686
                     /locus_tag="IVO69_002656"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7401391.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1750..2373
                     /locus_tag="IVO69_002657"
     CDS             1750..2373
                     /locus_tag="IVO69_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7401392.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2429..2815
                     /locus_tag="IVO69_002658"
     CDS             2429..2815
                     /locus_tag="IVO69_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7401393.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
BASE COUNT         1022 a          468 c          590 g          761 t
ORIGIN      
        1 gagatattct ttaggatatt aaaattaact agcaccacgg gtatcgagct ttctcttgaa
       61 tcttgggatc gagcagtggt tgaactgttt ccagaaggtt taatgatcga tacaaacgat
      121 gggaagatcc atctgactgc agcagaactg caaaaacaaa tgcgttatca aatagatgac
      181 gaaaattctt tttctatttt tagacgtcag caaaagattc taaaaagctt tttaaaagtg
      241 atcagtaaaa aacggaatct gatcaaaacg acacagctgt tgaaaaaata tccaggtctg
      301 cttcacacgt ctattcaatt tcctcaattg attacgatga tccatcgttt tatacgtatg
      361 caacagctac catctaagaa gctgtttctt ccattgacgg acacatttcg agtagtcaac
      421 agagaagata aaaagaaagt gattgtgatt gactttacca gaaatcggaa tgtccttcac
      481 caagttgcaa tggaaaaggc caattaaggc tttttctttt tggatttttt tgtcataata
      541 aatattagat gatggaggag ataatatgat gcaaacatgg ttagaaaggc taatacgatt
      601 tcagcagaac tatcacaaac ttatgcaggg aagatatgcg aaatttgatc agttgaatcg
      661 cggtctattg atcgctgcgc ttgtctttag tttgctaagt cgttggttgc cctatagaat
      721 cggacaattg ttgttcatcg tctttttcgg atggatgata tttcgtttcc tatcaaaaaa
      781 aatctacccc agactaaatg agaatcaacg ttatttgaaa tatcttgata gagtcaaaga
      841 aaaatatcat cagataaaaa acaaaaagca agtcaaaatg gatcgaaaaa gttatacttt
      901 ttttgagtgc ccgaattgtc atcaaaagca gcgtgcacct aaaggaaaag gacgaatacg
      961 tgtgacatgc aaaacatgtg gtataaaatt cgaaacgaat gtttaaggga gaaaagaatg
     1021 aaattagtat tattacgtca tggcgaaagc gaagcaaatt ttgaaaatta ctggacagga
     1081 tggctagatg tcgccttaac agaaaaagga aaagaacaag cagcagttgc cggtcaaaaa
     1141 atgcttgaag ccggacttca attcgatgcc gtttatacct ctgttttgaa acgtgcgatt
     1201 cttaccggac aagtcgcact agaagaaatg ggtcagttat ggcttcctat aataaaaaca
     1261 tggcgtctaa atgaacggca ttatggggat cttgtgggta aaaacaaaga taagatgaaa
     1321 gaaatatacg gaaaagatca agtaaaaaca tggcgcagag gatatacgga agttccgccg
     1381 ttaaccgcag aaaatagatt tgaccgcaga tatgatcact tggatccgca tttgatccca
     1441 tacggagaaa gtctggaaat gacagtgaat cggatcattc ccttatggca agatcatcta
     1501 gcaccaaaac taaaagatcg gcaagattta ctagttgtgg gtcatggtaa tagcatccga
     1561 gcactgacaa aatatttaga agatgttcct gaagatcaaa tggatacgat cgatatccca
     1621 aatgcacaac caatccagta tacattagca gatgatttga caattctgga caaaaaaata
     1681 ctttaaagaa cacctttatc taaaagtgag agatagaaaa aagaagcaaa ggaatggaac
     1741 aaaaggaaca tgaaaaatta tatatgggac tttgatggta cattgtttga tacgtatccc
     1801 gcaatggtag atggtgcatg gcaagcacta aaagattttg gtatcagtat ggacaaaaaa
     1861 gaaatctact tcaaaatgaa aaagtattct actagttatt tgatcaatga atcaaacttg
     1921 aatgccagag agtttaatga attatttcac cgatatgaaa aagaaagtac ggaagtttcc
     1981 cgtccatttc cagaaacgaa acaagtactg gaaatgctaa aagacaacgg cggtcgtcat
     2041 ttcatcctga cccaccgttt gacagaatca acatggggcc tgttaaaaga acatcgattg
     2101 gcacatctga ttgaggaagt agtagggatc gatcaggact ttcctcgtaa acctgatcca
     2161 gcatctttaa attatttgat cgatactttt catttagaaa gaacagacac gatgatgatc
     2221 ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag gagtcgctac ttgtctttac
     2281 gacatcgatc attttctggg tgagatcccg gcagattatg tggtaggaaa tctgaatgaa
     2341 atccttacct tgcagagcat cattaaaaag tagtatcccc tttcaaagga gatatgttat
     2401 aattttcata aaggagtgat aaaattatat ggaaatcaga agacaaaaag aagttgtaga
     2461 agcatttcat tatgatatga gattgccgaa ccaagaagta gaaacagatt tgaaagtagg
     2521 cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa gaacatagca tcgtaggagc
     2581 acgcttagag ttccgtttag tgtttgatac atatgtttta tctggaagtg tgagtcagat
     2641 caatcatatc ctcaatcgga aaattgaagg tcaaaatgat gtcacacaag aagaagtgga
     2701 cgagctagta gcaccgttat tcgatatcgt acgccgaatg tcttatgagg tgacagagat
     2761 cgccttagat cagcctgggg taaaattgaa ttttcaatca gaaaaacaag aatagacagt
     2821 aaaaacatac ctgcgataga t
//
LOCUS       DACCPY010000151         2786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCPY010000151 DACCPY010000000
VERSION     DACCPY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.162"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            54..656
                     /locus_tag="IVO69_002659"
     CDS             54..656
                     /locus_tag="IVO69_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401394.1"
                     /translation="MLKLISYAKKANSLSLVDNLDNLLPDKLIYDENEDNSQKQIIIF
                     ELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDD
                     LVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYD
                     PLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
     gene            complement(653..1276)
                     /locus_tag="IVO69_002660"
     CDS             complement(653..1276)
                     /locus_tag="IVO69_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401395.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            1594..1932
                     /locus_tag="IVO69_002661"
     CDS             1594..1932
                     /locus_tag="IVO69_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401396.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            2024..2765
                     /locus_tag="IVO69_002662"
                     /pseudo
     CDS             2024..2765
                     /locus_tag="IVO69_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a          427 c          483 g          871 t
ORIGIN      
        1 aatttttaga agaattatct ggagaagaat atagattatt cctaaataat aatattttaa
       61 aattaatatc ttatgccaag aaagctaata gcctatcttt agtagataac ttagataatc
      121 ttttacccga taaacttatt tatgatgaga atgaagataa ttcacaaaaa caaataatta
      181 tatttgaatt gtatttaaag tggcttagca gcctgtcagt gtcaccagaa agcaatgatg
      241 atgataaata ttttgcgaac aaactgtttc aatttatatt tccagactat tcttcaatgc
      301 cattttttac attaataaat tttatgctgt atgttaaaga acacttagga tatgatgatt
      361 tagtagaaat gataaaggca tatgataaag aaccttatcc attcaaagga atcaatactg
      421 gttcagatat ggctatgatt tcaggcgaaa ctgtagaaga aattctagct aattacaaag
      481 aacaaaatat aaaaactctg gaattaatta aaaagaatat aaaatactat gatcctttac
      541 tagcgaataa agaaaatatt gataagttat taaatgcaat tgctgattct gatgttagat
      601 ctcctagaat tgatgagtta aaagaagaaa ttttaatgta taaagaactt ttttaattca
      661 tttgattact gttatactca atatcattct taattctgta aatagtttgt ctagtaatat
      721 tgtagttttt agcaattttt gaaatagcta tgccctcatt taaatcctta acgatgtttt
      781 cataaactaa acgtcgttga ggattttttg tatctgcact atagagtttt ggacgacctt
      841 tatagacgcc atttgctttt gcaattttaa tgacttgcgc ctggcgtcgt ttagattcgg
      901 tacgctcttg ctctgcaatc atggccaaaa tttggatgat taaatcttta atgaatttat
      961 ccaaatgagg attgccaatt gcttcagcca tgatcggtag actggtgatg atcagctgca
     1021 cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt cttccaagtc
     1081 ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa gcttcctgga
     1141 aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt ttttctgcgc
     1201 cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac gaaactcgaa
     1261 tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata cactcaaaat
     1321 caacactaat ttttttatac actataaagc tgtcattttt ctagataaat ggcataaatt
     1381 ttcagcgcta agtgacaaaa gcaactaatt tttcatgata gatttttaaa tatctcaatc
     1441 tatctccaaa attagttgct tttgtagcta tagttgacta tgaatatgac aaataattag
     1501 aaaaaactgt gccaattaaa tatcaaaatg acaaattgat gaatcaaatt caaattaagg
     1561 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
     1621 aggcgtttga ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga
     1681 aaaaaaatta aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc
     1741 aaaggtcggc aactgaaatt taaaaaaaat gatccacgtt taaagcatgc tttcgatttg
     1801 tttttgaatg gtttatccga taaagaagtt gaagaacaaa ctgggatcaa tcgccgaacg
     1861 tttagaaggt atcgagcaag atataacgtg acagtagatc aaagaaaaaa caatgaaaag
     1921 agggatagtt aatgagtacg gttattttag ctgaaaaaca agccaggcat tagcctacgc
     1981 aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag acccactatt
     2041 tacagatgaa acgtttatca cctttgattt tgggcattta gtggaattag cagaaccagg
     2101 tcattatgac gaaaagtggc aaaattggaa acttgaatct ttgccgattt ttcctgatcg
     2161 atacgatttt gaagttgcaa aagataaggg aaagcagttt aaaattgttg cagaacttct
     2221 caaaaaggca aatacaatta ttgttgcaac agatagcgac agagaaggtg aaaatatcgc
     2281 ctggtcgatt atccataaag caaatgcctt ttcaaaagat aaaacattta aaagactatg
     2341 gatcaatagc ttagaaaaag atgtgatccg aagcggtttt caaaatttgc aaccaggaat
     2401 gaattactat cccttttatc aagaagcgca aacacgccaa attgccgatt ggttgatcgg
     2461 catgaacgca agccctttgt atacgttaaa tttacaacag aagggcgtac aaggtacatt
     2521 ttcactagga cgtgttcaaa cgcccacctt ataccttatt tttcagcgcc aggaagctag
     2581 tataaaagta aaacaagggt catttaaggg cgttctaagc cccacacagc gttttaaaac
     2641 ccaagaggag cttttttgct tttgtttctt ctaaacaagc taaaataggc aatcaagagg
     2701 ggataattgc tgatgttcaa accaaagaga tggataggct acaattaact agacagaaaa
     2761 attaaggtgt gtagactaga agaaaa
//
LOCUS       DACCPY010000152         2646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCPY010000152 DACCPY010000000
VERSION     DACCPY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.163"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(40..1146)
                     /locus_tag="IVO69_002663"
     CDS             complement(40..1146)
                     /locus_tag="IVO69_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7401397.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="IVO69_002664"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="IVO69_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7401398.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT          832 a          498 c          548 g          768 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttag caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat atcctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aataggagca cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtgggttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatataat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggatgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag
     2161 aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgtgcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc cttttcttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatagaaaa caatccgtgt atatatgctg tattttgttc accaccatca aaaaaataag
     2641 ggaatg
//
LOCUS       DACCPY010000153         2635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCPY010000153 DACCPY010000000
VERSION     DACCPY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.164"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            473..1294
                     /locus_tag="IVO69_002665"
     CDS             473..1294
                     /locus_tag="IVO69_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401399.1"
                     /translation="MYQFLKTGATILNYYQQYKENKKRCGRRSIALPEEQKEYVQKRV
                     VQGWTPDVIVGRSEMKLDCSVRTLYRMFQTGAFNVRDLPMKGKRKPNGHQEKRGKQAF
                     RRSIHERIKEHPEFENEFGHLEGDTIVGRKHKSAVITLVERLSKVIITLKPAGRQAMD
                     IENRINHWFQTVPKHLFKSITFDCGKEFSNWKAMGNQNDIDIYFADPGTPSQRALNEH
                     SNGLLRRDGLVKAMDFNEVSESFIEYRKHFLYSILSSFKFSIDYIYCEFCRGSET"
     gene            1381..1986
                     /locus_tag="IVO69_002666"
     CDS             1381..1986
                     /locus_tag="IVO69_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7401400.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            2002..2574
                     /locus_tag="IVO69_002667"
     CDS             2002..2574
                     /locus_tag="IVO69_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401401.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLNDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
BASE COUNT          944 a          416 c          525 g          750 t
ORIGIN      
        1 aataacaact atgaaatata gagtatgtaa atagcatgtc tataaaaaat aaagtagtaa
       61 attaatcata atcttttcaa caacaggaat agtcaagtta gactgttcct gttgtttctt
      121 tattttcctt atcatcttca actttttcaa atactgcaaa tacaaaatat caatataact
      181 tattcgttct atcaatcagt tcctaatatc tactcatcaa aatgaaattt caaactaatc
      241 tcatcatagt attcttttcg tcgattgtaa aattaagcta gacaactaaa aagatcattt
      301 gtgctacact caaaatagtt ccgaccaaag aattattaag gagtgagtac aaatgaccta
      361 cacccatctt acaacagacg aactggtaat catagagtct tatttcaaaa ataatcaatc
      421 cgttgccaaa actgcgcaat gcttaggacg tgcgtgacaa acgattcata acgtgtatca
      481 atttttgaaa actggtgcta cgatactgaa ctactatcaa caatacaaag aaaacaaaaa
      541 acgctgcggt agacgctcga ttgctttacc agaggaacaa aaagaatatg ttcaaaagag
      601 ggttgttcaa gggtggacac cggatgtgat tgtcggccgc tcggaaatga aacttgattg
      661 ttcagtccgt acactgtatc gaatgtttca aactggcgca tttaatgtgc gtgatttgcc
      721 tatgaaaggc aaacgaaaac caaatggaca ccaagaaaaa cgtgggaaac aagcttttcg
      781 gcgcagtata catgagcgca tcaaggagca tccagaattt gaaaacgagt ttggccatct
      841 tgaaggagac actattgtcg gtcgaaaaca caaaagtgct gttattacat tggtggaacg
      901 tttgtcaaaa gtgattatta cattgaagcc agccggtaga caggcaatgg acattgaaaa
      961 tcgaattaat cattggtttc aaacggttcc taaacatctg tttaaatcaa ttacgtttga
     1021 ctgtgggaaa gaattttcaa attggaaagc tatgggaaat caaaatgata ttgatattta
     1081 ttttgcggat ccaggcaccc cgtcacaacg cgctctaaat gaacattcaa atggattatt
     1141 acgtcgtgat ggattagtta aagccatgga cttcaatgaa gtatcagaat catttataga
     1201 gtacagaaaa cattttctgt actctatttt gtcttctttt aaattttcaa tagattatat
     1261 ctattgtgaa ttttgtagag ggtcagaaac ttaatagttt ttgacacata aacaaaatcg
     1321 cgatataatg gggttgagag tgtttcataa acttatgtca taaagttaag gagatttcgc
     1381 atgattttag agaataaatt gggattaacc aatcaagttg agttggctaa ggttgaagaa
     1441 aaactcagta aacaaaaagc gaaagaattg tacgattgtg gaaaaataaa tgaaatcgaa
     1501 gttggtacat ttaaaggttt atctgaaatt catgaatttc tattttcgga tatttacgat
     1561 tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa attttcgatt tgcaccagtt
     1621 atgtatttag aacattcgtt acagcatatt gatcagatgc cacaaacttc ttttgaagag
     1681 attatcaaga aatatgtgga gatgaatatc gctcacccat ttcgagaagg aaatggccga
     1741 agtactagaa tttggctgga tctcatttta aaggaagaat tgcaaaaagt tgtagattgg
     1801 aacttaatcg ataaagcaga ctatctttct gcaatggagc gaagtcccat aaacgatttg
     1861 gaaatacgct atctaatttc caatgctctg actgataaaa ttgacgatcg cgagttatat
     1921 atgaaaggaa tcgatgtcag ctatttctat gaagggtatt ctgagtatac gatagatgat
     1981 ttgtaaaagt aggtggacca aatgacgaaa tatggatatg cacgtgtctc aaccgcttct
     2041 caaagtacaa aagaacaaat tcaacagttg cttgagaacg gcgtagagaa gaagaatatt
     2101 ttttcagaaa aatttacggg tacaaaacgg gatcggccaa aattcaatca gctgttggct
     2161 ggtctgaagc ctggagatga aatagttgtt acgaagcttg atcgatttgc tcgtaacacg
     2221 agagaagcat tagagatcat tgaaccttta ctcaatgata acgtaacgat caaggttttg
     2281 aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg tgacacggac gttgttgagt
     2341 gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc aagaaggaaa aatgtttgca
     2401 agaaagaata atcctaattt cagagaagga cgtccgaaag caacaatcac accgaagaag
     2461 aggcacgcat atgaactgct tacttcaggt aaatcgtata aggaagttga agctataacc
     2521 ggtttcagtc gcagtacgct ttttagaata aagaagaaaa ttgaagaaag ttaaaaaaga
     2581 agtctcagcg aaccagacag gtaaatactt aatgcgttct agatatgagt ctatt
//
LOCUS       DACCPY010000154         2630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCPY010000154 DACCPY010000000
VERSION     DACCPY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.165"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IVO69_002668"
     CDS             complement(61..2424)
                     /locus_tag="IVO69_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP7401402.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          894 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
//
LOCUS       DACCPY010000155         2613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCPY010000155 DACCPY010000000
VERSION     DACCPY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.166"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IVO69_002669"
     CDS             316..570
                     /locus_tag="IVO69_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7401403.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVO69_002670"
     CDS             571..888
                     /locus_tag="IVO69_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7401404.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IVO69_002671"
     CDS             1447..2382
                     /locus_tag="IVO69_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401405.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..>2613
                     /locus_tag="IVO69_002672"
     CDS             2476..>2613
                     /locus_tag="IVO69_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7401406.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          958 a          375 c          483 g          797 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcataga gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aag
//
LOCUS       DACCPY010000156         2553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCPY010000156 DACCPY010000000
VERSION     DACCPY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.167"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            122..1417
                     /locus_tag="IVO69_002673"
     CDS             122..1417
                     /locus_tag="IVO69_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7401407.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1597..1681)
                     /locus_tag="IVO69_002674"
     tRNA            complement(1597..1681)
                     /locus_tag="IVO69_002674"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1645..1647),aa:Leu,seq:caa)
     gene            complement(1699..1770)
                     /locus_tag="IVO69_002675"
     tRNA            complement(1699..1770)
                     /locus_tag="IVO69_002675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Gln,seq:ttg)
     gene            complement(1780..1852)
                     /locus_tag="IVO69_002676"
     tRNA            complement(1780..1852)
                     /locus_tag="IVO69_002676"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1817..1819),aa:His,seq:gtg)
     gene            complement(1914..1998)
                     /locus_tag="IVO69_002677"
     tRNA            complement(1914..1998)
                     /locus_tag="IVO69_002677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1962..1964),aa:Leu,seq:caa)
     gene            complement(2019..2089)
                     /locus_tag="IVO69_002678"
     tRNA            complement(2019..2089)
                     /locus_tag="IVO69_002678"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2055..2057),aa:Cys,seq:gca)
     gene            complement(2135..2206)
                     /locus_tag="IVO69_002679"
     tRNA            complement(2135..2206)
                     /locus_tag="IVO69_002679"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2172..2174),aa:Gln,seq:ttg)
     gene            complement(2218..2290)
                     /locus_tag="IVO69_002680"
     tRNA            complement(2218..2290)
                     /locus_tag="IVO69_002680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2255..2257),aa:His,seq:gtg)
     gene            complement(2293..2366)
                     /locus_tag="IVO69_002681"
     tRNA            complement(2293..2366)
                     /locus_tag="IVO69_002681"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2332..2334),aa:Trp,seq:cca)
     gene            complement(2373..2453)
                     /locus_tag="IVO69_002682"
     tRNA            complement(2373..2453)
                     /locus_tag="IVO69_002682"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2417..2419),aa:Tyr,seq:gta)
     gene            complement(2455..2530)
                     /locus_tag="IVO69_002683"
     tRNA            complement(2455..2530)
                     /locus_tag="IVO69_002683"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2495..2497),aa:Phe,seq:gaa)
BASE COUNT          784 a          557 c          513 g          699 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     1381 tatgattcgt catggcaaaa catttttaac caaataaaca gaacagaaag aggttttctt
     1441 ctttctgttc tgtccaaatt attctaagat ttctctttac caacatcatt tgacaaagag
     1501 ccgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag
     1561 caactctcta taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg
     1621 cacgtcctca acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
     1681 caaatttaaa aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag
     1741 tcccttgcct taccgcttgg ctatacccca agaataaagg gcgaatgatg ggaatcgaac
     1801 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatcataa
     1861 aagatgttat ttaatttctc aataaaaaac taagatgaag acatcttagt caataccggc
     1921 ggccggggtc gaaccggcac gccctcaacg ggcactggat tttgagtcca gcgcgtctgc
     1981 caattccgcc acgccggcaa gttgatttat tagtactaag gcggtaaccg gatttgaacc
     2041 ggtgatgaag gttttgcaga cctctgcctt accacttggc tataccgcca ttatactatt
     2101 atcaatctat aaaataaaga ataaattata gaaactgggg tagctggatt cgaaccaacg
     2161 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaagggc
     2221 gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg
     2281 ccacattcgc catggcaggg gcagcaggaa ttgaacccac actaacggtt ttggagaccg
     2341 ctgttctacc tttaaactat gcccctataa aatggaggag agtggattcg aaccactgaa
     2401 ccctaaggaa cggatttaca gtccgtcgcg tttagccact tcgctactcc tccatggtgg
     2461 cgcgggacag aatcgaactg ccgacacacg gagcttcaat ccgttgctct accaactgag
     2521 ctaccgcgcc atattttaaa attattaatt gaa
//
LOCUS       DACCPY010000157         2475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCPY010000157 DACCPY010000000
VERSION     DACCPY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.169"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            280..543
                     /locus_tag="IVO69_002684"
     CDS             280..543
                     /locus_tag="IVO69_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7401408.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            537..887
                     /locus_tag="IVO69_002685"
     CDS             537..887
                     /locus_tag="IVO69_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7401409.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(911..1525)
                     /locus_tag="IVO69_002686"
     CDS             complement(911..1525)
                     /locus_tag="IVO69_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401410.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1839..2261
                     /locus_tag="IVO69_002687"
     CDS             1839..2261
                     /locus_tag="IVO69_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401411.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2271..2474
                     /locus_tag="IVO69_002688"
     CDS             2271..2474
                     /locus_tag="IVO69_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401412.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          872 a          364 c          419 g          820 t
ORIGIN      
        1 attttctatg tcctttttat tccattttgt atcaataact atgaaatctt taaagtatcc
       61 agaagagatt atttttagat caattttttt cttacctgat tctaaagatt ttgttttctt
      121 aaacatatta tgatttcctt tcgactattt tcatataaat gttaacatag tttatggatt
      181 catggaatgt taatctaatt taatagaaaa ggataattaa gtatatacgt tttttcttat
      241 gtatgttata ctatgtatat acagaaagag gtgtctacta tggaaatgtt acctataaaa
      301 aaatggggaa atagtaatgg ccttagattg ccaaagtata ttatggaata tcttggaatt
      361 catacagaag acaaagtgaa gataatacaa gaagaagtaa acggtcagaa gcggctaatt
      421 atcgaagcgg ctaatataga aaatgaatta acaattgaac agttatttga aaattacact
      481 gatgaaagaa atcatgtgac gattcaaaat ttaggtgaag cagtaggtaa tgaaaaatgg
      541 tgagaaaacc tagacaaggc gatattctat tattgaatac agcacctcga tctggacacg
      601 aacagacagg taaaagacct tatattgttt taagtcatga tattattgcg gattatagta
      661 acgttgttac agttgctcca atttctagta caactagaaa ctatccgcta tatgtaaata
      721 taaactcaga atatgaaatg aagacaacag gaaaagtatt attagatcaa ctaacgacaa
      781 ttgattatga agccagagag tgtatttttt tggaaaaagc gcatgatagt ttagttgagg
      841 agttattgat gaaagttaga acagtttttc aaaaagtaag caaataaaat agaaacagca
      901 gcatgatcaa ctattcgtga ttgaccatgc tgtctttttt tattctatat acggtttgtc
      961 tcgtaacatt gtagtctttt gcaatcttag cgatcgcaac accttttttt agatcctcaa
     1021 cgatgttttt ataaacaaga cgacgttggg gatcttttgc attggcactg tatagcttag
     1081 gacgtccttt ataaacaccg ttcgcttttg caatcttaat gccttgtgcc tgacgtcgtt
     1141 tagattcggt acgttcttgt tcagcgatca tggccaaaat ttgaatgatc aaatctttaa
     1201 tgaatttatc caggagagga ttgccaattg cttcggccat gatcggtaga cttgtgatga
     1261 tcagctgcac atttttcttc ttcaagtaat tgactgaagc gatgatctcg tcgtaatttc
     1321 ttccaagtcg atcaatagct tccacaataa aaatatcttg atctcggacg aaatccagtg
     1381 cttcttgaaa aacaggtcga tgagtaatcg ttgcgccaga ttgtttctca atgaaaattc
     1441 tctctgcacc gaattttttc atttcttcaa tctgccgttg ttcattttga tcgatggatg
     1501 agacacgtat gtatgcaact ctcaaaattt ttacctactt tctgcaacta tttaaaaaac
     1561 accctaatca tacacgagaa aaccagattt ttcaatgtcg cggtcgtgtc tagttagggt
     1621 ataccttata tagacaaaaa ctaatgatgg aggaaatagt atgtgtcact tttcttttta
     1681 aatggcaata ttttttgtaa ataaatgtca atcaagaaaa tttaaacgat tatgacatta
     1741 aaaatatatt ttatgaattt tctagtatat aacttgattt atctgttaag atttattaag
     1801 aaaacgtttt cttatttttg atttcgaggt gatagacaat gaaaaataag tttattttat
     1861 ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct acatttgctg
     1921 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
     1981 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
     2041 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
     2101 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
     2161 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
     2221 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
     2281 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
     2341 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
     2401 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
     2461 aaaaagaaaa ataat
//
LOCUS       DACCPY010000158         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCPY010000158 DACCPY010000000
VERSION     DACCPY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.170"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IVO69_002689"
     CDS             207..2069
                     /locus_tag="IVO69_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7401413.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IVO69_002690"
     CDS             2069..>2398
                     /locus_tag="IVO69_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7401414.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACCPY010000159         2269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCPY010000159 DACCPY010000000
VERSION     DACCPY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.171"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(62..508)
                     /locus_tag="IVO69_002691"
     CDS             complement(62..508)
                     /locus_tag="IVO69_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7401415.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(535..711)
                     /locus_tag="IVO69_002692"
     CDS             complement(535..711)
                     /locus_tag="IVO69_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7401416.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(873..1301)
                     /locus_tag="IVO69_002693"
     CDS             complement(873..1301)
                     /locus_tag="IVO69_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7401417.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1393..2253
                     /locus_tag="IVO69_002694"
     CDS             1393..2253
                     /locus_tag="IVO69_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7401418.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          672 a          454 c          368 g          775 t
ORIGIN      
        1 ttcttttttt acgaacacaa aaaactgcgg acaatcactg tccgcagttt aaatgataaa
       61 attaagattg taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt
      121 tgactttagg cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag
      181 tttgagtgat tgcttcttga acaagttgtc gaatttcttc atcggaaagt tgttccggca
      241 tatatttttc gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg
      301 cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta
      361 gttcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact
      421 tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt
      481 catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa
      541 ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta
      601 gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg
      661 acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc
      721 ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga
      781 ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt
      841 taaaattatc aaatgttatt ttatttaaac ccttattgac aattttcaca tcttccgaaa
      901 atctcgaatc tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc
      961 ggacacatat ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg
     1021 ccgatttccc cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct
     1081 tccagtaaat cgatctctgc aaaatcatgg agattccgat aaatcgtatc ataacttacc
     1141 cctttgaatt cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc
     1201 ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc
     1261 aatgtttcta atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca
     1321 aaatgattac gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt
     1381 gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac
     1441 tctgctggag aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt
     1501 gagttcaacc tattccgcag aacgttcgtg acagatgaaa aaacattaaa gcaggcattg
     1561 caagatgcat tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc
     1621 gagataacac gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca
     1681 ccgatatcag aaattgaacg tttaaccggt atcaagccaa ttcggcaacc tggtgccctc
     1741 catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac
     1801 gatgatggga aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc
     1861 cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat
     1921 ttacctttga tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa
     1981 atcgtgggac taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc
     2041 tcttacggat taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactc
     2101 gattttgcga atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta
     2161 tctattgaag aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat
     2221 tatggaactg aaacaacaga aaaaagcgat taatcatttt tgtcttttt
//
LOCUS       DACCPY010000160         2173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCPY010000160 DACCPY010000000
VERSION     DACCPY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.172"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            116..2023
                     /locus_tag="IVO69_002695"
     CDS             116..2023
                     /locus_tag="IVO69_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7401419.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT          744 a          409 c          438 g          582 t
ORIGIN      
        1 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
       61 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
      121 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
      181 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
      241 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
      301 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
      361 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
      421 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
      481 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
      541 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
      601 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
      661 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
      721 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
      781 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
      841 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
      901 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
      961 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
     1021 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
     1081 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
     1141 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
     1201 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
     1261 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
     1321 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
     1381 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
     1441 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
     1501 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
     1561 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
     1621 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
     1681 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
     1741 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
     1801 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
     1861 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
     1921 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
     1981 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
     2041 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
     2101 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
     2161 gctgcttagt ttc
//
LOCUS       DACCPY010000161         2152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCPY010000161 DACCPY010000000
VERSION     DACCPY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.173"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(682..972)
                     /locus_tag="IVO69_002696"
     CDS             complement(682..972)
                     /locus_tag="IVO69_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401420.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1180..1872)
                     /locus_tag="IVO69_002697"
     CDS             complement(1180..1872)
                     /locus_tag="IVO69_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7401421.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          619 a          397 c          313 g          823 t
ORIGIN      
        1 tataacatag ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa
       61 ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat
      121 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
      181 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
      241 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
      301 tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
      361 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
      421 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
      481 attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc
      541 atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct
      601 cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt
      661 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
      721 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
      781 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
      841 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
      901 gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata
      961 ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag
     1021 aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg
     1081 cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg
     1141 ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc
     1201 aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt
     1261 ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt
     1321 atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt
     1381 caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc
     1441 tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct catctagttt
     1501 gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc
     1561 taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt
     1621 ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg
     1681 agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag
     1741 attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta
     1801 ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa
     1861 tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat
     1921 ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa ttttaatata aatactatca
     1981 aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaatg
     2041 caggttaata ctttcttaaa taaatattta tggtgaacaa aaacaaaaaa gcaagtttta
     2101 ttaaacgtaa cccttttcat ttcatagatt actgtattta ttacaaaaaa cg
//
LOCUS       DACCPY010000162         2145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCPY010000162 DACCPY010000000
VERSION     DACCPY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.174"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(118..708)
                     /locus_tag="IVO69_002698"
     CDS             complement(118..708)
                     /locus_tag="IVO69_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7401422.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IVO69_002699"
     CDS             1231..1392
                     /locus_tag="IVO69_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401423.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IVO69_002700"
     CDS             1411..1905
                     /locus_tag="IVO69_002700"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP7401424.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          774 a          332 c          364 g          675 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaa
//
LOCUS       DACCPY010000163         2106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCPY010000163 DACCPY010000000
VERSION     DACCPY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.175"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(385..458)
                     /locus_tag="IVO69_002701"
     tRNA            complement(385..458)
                     /locus_tag="IVO69_002701"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Ile,seq:cat)
     gene            complement(647..1666)
                     /locus_tag="IVO69_002702"
     CDS             complement(647..1666)
                     /locus_tag="IVO69_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7401425.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1677..1883)
                     /locus_tag="IVO69_002703"
     CDS             complement(1677..1883)
                     /locus_tag="IVO69_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7401426.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(2016..>2106)
                     /locus_tag="IVO69_002704"
     CDS             complement(2016..>2106)
                     /locus_tag="IVO69_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401427.1"
                     /translation="NIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          633 a          425 c          344 g          704 t
ORIGIN      
        1 aaatcaaaaa taagtcacta attaactacc actccaattg taagcctttt ttctcacttt
       61 ttttcaaaaa tatggtatgc cttttaatag cttcaaatat aaaagagttt aaagcgtaac
      121 acacttatgg ggaagtggct tggggtgcgc tttaaactct tctttattat catctcacaa
      181 tttaacccaa atgtctttct atttaaaaat caaagtaaaa cttttcaaat atacagaaat
      241 ataactatgt gaaacatcct ttcattaatc cataaaagga tacataaaaa agccactcat
      301 ttgagtggca atgaagaaaa gctttagctt gtataatact cttcaaaaaa ttctaacaca
      361 gaacgattca aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt
      421 tatgagccgt ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca
      481 taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg
      541 actattatgg gtgatagtga agatatgcga taacatcact attttatcga atgattttta
      601 tagttgtcaa tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa
      661 tatttaaata ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg
      721 gatcattcgt tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa
      781 aagatacatg atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat
      841 aaattccatc agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga
      901 tatactcgta tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga
      961 tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg
     1021 caccagctcg tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg
     1081 taaatccatg agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat
     1141 aaccgtaagg atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga
     1201 ttcccggttg gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat
     1261 ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt
     1321 gttgtaactc aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta
     1381 cataaccagg ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat
     1441 aagcgctagt ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat
     1501 tagccgtttc atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg
     1561 cgcccaaatt aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa
     1621 gagccgttaa caggctcatc gcagtaatag taattttctt tttcatatct atcctcctat
     1681 ttatctgatg aaattttctt atacgttcga tttgatacac ctaaaatact ccctagaaat
     1741 gctccaaaac ctgtaataat catcaccgtt atatctgtgt accgccagtt aacacctttt
     1801 ccgacaagac cgacaaaagt cgctaaagcg gggataacta ctatagcgat ccatttcaaa
     1861 atatcaaagg ttctattgtt catactatcc acctcccttc attggttggg ctactttctt
     1921 tttcaatcgc ttgttttctt ctatcaatat ttctctttct gaaagcaagg catcaatttg
     1981 cccgtccagt tgttcaattt tatctttctt aattgtcaaa ttaagttaga cagatcatca
     2041 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
     2101 atatta
//
LOCUS       DACCPY010000164         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCPY010000164 DACCPY010000000
VERSION     DACCPY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.176"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVO69_002705"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVO69_002705"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7401428.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IVO69_002706"
     CDS             complement(1431..1823)
                     /locus_tag="IVO69_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7401429.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgcac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACCPY010000165         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCPY010000165 DACCPY010000000
VERSION     DACCPY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.177"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            8..1201
                     /locus_tag="IVO69_002707"
     CDS             8..1201
                     /locus_tag="IVO69_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7401430.1"
                     /translation="MRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLTSSQQKGIV
                     LQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGIISYFYPVF
                     TLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEEEQQKIGTF
                     FKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGELG
                     KTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPIKD
                     VPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPRYS
                     QVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKSFL
                     QKMFV"
     gene            1214..>2031
                     /locus_tag="IVO69_002708"
     CDS             1214..>2031
                     /locus_tag="IVO69_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401431.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT          707 a          330 c          413 g          581 t
ORIGIN      
        1 accagaaatc cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt
       61 cgttgtagat tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc
      121 tcaacaaaaa gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga
      181 aaataatatt ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga
      241 taatgatgtg tttgtgttta accgtaatga cattattgat agagggatta taagctattt
      301 ttatcctgta tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa
      361 tggaatccaa cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct
      421 aaaaaagttt aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg
      481 caccttcttc aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct
      541 gaaggaaacg aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc
      601 agaaatccgg tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg
      661 taaaactcaa tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc
      721 attttttaaa gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa
      781 tcattatgta tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc
      841 agcgattata tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac
      901 aagccctttc ttaatagata acaataccat ggcttattta tttaataata cttgggatat
      961 ctattttggt aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc
     1021 attgccttct tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga
     1081 tgaacaacaa aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca
     1141 acgtaagtta gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta
     1201 ggagggaaga agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat
     1261 tcgtgtactt ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga
     1321 agacctttgg gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga
     1381 ttttcctttg accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa
     1441 aaccccattt gatgctgcga aatggttaaa aggtgaaaat gggatggctc ggatcacgat
     1501 tgaacgggaa gattcgcaat tgggttctgt ctcacttatc ctttattcca atcaagacat
     1561 tggtggagga atctctactt atgaagtggt ccatcaaatt gcaaaacgtg gaagtaacat
     1621 agaagcacgg gatcgacgtt ttgacgtcac cttattaatc aatggattac cgattgtcca
     1681 aatcgagctg aaacaagtaa ctgcaaaaga tggtgtttat caggcattta accaaatcaa
     1741 aaaatatgca gaagaaggta tgtttcggaa caatatcttt tcgaccttac agttgtttgt
     1801 ggtttccaat gaacaaacca ctcggtattt tgcaaatgcc ttaccgaaag acttgcaccc
     1861 taagttttta tttagttggc gaacgaaaga caatgaaaaa gtagaaaact tatatgagtt
     1921 ttgtaaacaa gtgttaaata tcccggatgc tcatcgtttg attgcggatt atacaatcgt
     1981 cagtgaagat caagacaata aaaccctcat ggtgttacat ccttatcaag t
//
LOCUS       DACCPY010000166         2013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCPY010000166 DACCPY010000000
VERSION     DACCPY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.178"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IVO69_002709"
     CDS             287..694
                     /locus_tag="IVO69_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401432.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVO69_002710"
     CDS             708..1109
                     /locus_tag="IVO69_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401433.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVO69_002711"
     CDS             1111..1482
                     /locus_tag="IVO69_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401434.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVO69_002712"
     CDS             complement(1518..1820)
                     /locus_tag="IVO69_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401435.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          761 a          270 c          336 g          646 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtg
//
LOCUS       DACCPY010000167         1978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCPY010000167 DACCPY010000000
VERSION     DACCPY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.179"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            129..1853
                     /locus_tag="IVO69_002713"
     CDS             129..1853
                     /locus_tag="IVO69_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7401436.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          773 a          242 c          347 g          616 t
ORIGIN      
        1 cagtttgtaa caaacatata ttttttatat tacaatattt caacaaatta aaaaaaaatt
       61 atatcttctt aaacaatgat aatattaaaa tgcaagcgct tgtaaaaaat aaggagaaga
      121 ggtaaaaaat gaagaaaaaa ctattaactt tatttcttgt gatgtctttc tcaatcgttt
      181 tgagtgtgta ctacaatact attgtttttt cagaacaaat aaactacaca gaagaacagt
      241 ataatataga aaaagaaaaa gctttaaaag ttggttatag tgaagaacag tttaagcaga
      301 ttatggaaat acctacaaat ttatcaaatg aaaatagtga aacaagaata gtaaattata
      361 ctatgacttc taatcaaacc aaagtcatta ataaagcaat ggagcaaatt ggaaagccat
      421 acgaatgggg agcatcggga ccaaattctt ttgactgtgg tggtttagtg aaatatgttt
      481 acaaacaagc tgtaaatatt gaactgccta tgggaaccac caatcaagag caatacggta
      541 cagaggtaag tttaaattca cttaagcctg gtgatttatt attttacggc aatcgaggtg
      601 caacttatca tgtaggtata tataaaggta acggtgtaat gattcatgca ccgcaacctg
      661 gagaaactgt aaaagaggtt aacatacaat atttttatcc taactttgca aaaagaattt
      721 tgcctgatga acctgactac ccttatattg actataataa aatggtaact gttacaaaag
      781 cttggtcaat atggaatgat ttacaattta gtcatgaaat taagaaagct attattggag
      841 ataattacaa gatagggaag gtttatacta atccagaaaa taataataaa tatggagaaa
      901 tattagtaag taataaagta tatggataca ttaattttta tgctgttaaa gagttgacct
      961 ctgttcaaat aaacagatat ttaacttcta aagatagtgg tcaaccaact tggggaaatc
     1021 tagagtgtac tataagtaaa ggacaaacta caaaagataa aatatatttt gttaaaggag
     1081 catataattt aggggatgga aagtatctat attctattta taaagatcag gattcctctg
     1141 aatggttagg gtatttaaaa gcacatgtta gtttagccta tactcctatt gaagaaatta
     1201 ataaaaacgt aactgttact aaaaattggt cgatttggaa taatctacaa tgggaaaagg
     1261 aaattgaaaa gcctcagatt ggttctgtat tttcagctag attaaagtta accaatgtat
     1321 cgaataatgc tgtctattac aagctttata aatctggaaa attttatggt tatatcaatg
     1381 ctgaggctgt caaagacctt acaacaacaa agcttaataa gtatgttaca tttagtgtta
     1441 ataatgaaga tttttggtct tctttagatg taaattattc aaaaggaaaa actgaaagag
     1501 gccgagtatt ttacattagt atttcatata ataccgcaga taaccaaccg atttattcag
     1561 tatatacaga tgaaacatgt acggaatggc gagggtatta taagggaaac aactttgaag
     1621 acactcaaat tactatgctt gagaataaaa gcgtgaaagt aacaaaaagt gggtatacag
     1681 tatggggaga cttgaatttt tggactaagt caggaatatc gaacacagga gatatataca
     1741 ccactgaccg aaaattttat aattttacca acaatgctta ctattatgaa ttgaaaaaag
     1801 atgggagggt ctatggctat ataaatagtg aagcagctgt agagatgaac taaaatattg
     1861 ctgtccctta atcttatggg gcagcaatat ttttatatta ttaatcattt ttctgaagcg
     1921 agggtagaag aattaatcaa tagtttttct tgttttgtaa tataatagtt taattacc
//
LOCUS       DACCPY010000168         1761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCPY010000168 DACCPY010000000
VERSION     DACCPY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.181"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(117..731)
                     /locus_tag="IVO69_002714"
     CDS             complement(117..731)
                     /locus_tag="IVO69_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7401437.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1337..1714)
                     /locus_tag="IVO69_002715"
     CDS             complement(1337..1714)
                     /locus_tag="IVO69_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401438.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          575 a          354 c          282 g          550 t
ORIGIN      
        1 aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt ttttcaaacg
       61 ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt ggataactat
      121 tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa
      181 aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg caaagtcaag
      241 actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc
      301 aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt aacaatgatt
      361 aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg gtttcttcct
      421 tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc aataattgcc
      481 tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat caatatactt
      541 ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag tgtgcggcta
      601 gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt gccagcttga
      661 agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata gtcaaaataa
      721 cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa cagctgccta
      781 atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt ctttgctgtt
      841 tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc aaataaaaca
      901 acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag attaggtaca
      961 ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt
     1021 gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct ttttttattc
     1081 agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag caagaaaata
     1141 caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg aatggcttat
     1201 cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa aaagcttttt
     1261 tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat ctttttttgc
     1321 gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg agagaatctg
     1381 tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt cttttcatat
     1441 atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg caagcttccc
     1501 aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct gcataccaga
     1561 taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat agactaataa
     1621 gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg agtatatcaa
     1681 gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt tttttataat
     1741 taaaaaattc gcctttttat t
//
LOCUS       DACCPY010000169         1733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCPY010000169 DACCPY010000000
VERSION     DACCPY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.182"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            36..1208
                     /locus_tag="IVO69_002716"
     CDS             36..1208
                     /locus_tag="IVO69_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7401439.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(1370..>1733)
                     /locus_tag="IVO69_002717"
     CDS             complement(1370..>1733)
                     /locus_tag="IVO69_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401440.1"
                     /translation="ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEA
                     IAQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPAT
                     LSEAQARGLTPCSKCYGN"
BASE COUNT          549 a          302 c          357 g          525 t
ORIGIN      
        1 ctcttcatat acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac
       61 tggacatctt catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg
      121 tagaagattc actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag
      181 catcgaataa gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc
      241 aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg
      301 caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca
      361 tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt
      421 tgcaaaaagg aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc
      481 atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta
      541 agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag
      601 tagaaaaaat ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca
      661 gagatctcgt gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg
      721 tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg
      781 atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg
      841 gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc
      901 ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg
      961 taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt
     1021 ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac
     1081 atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga
     1141 aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga
     1201 aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa
     1261 gctgacgaaa tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct
     1321 atttttttat gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat
     1381 aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat
     1441 acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag
     1501 tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg
     1561 ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     1621 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     1681 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tca
//
LOCUS       DACCPY010000170         1672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCPY010000170 DACCPY010000000
VERSION     DACCPY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.183"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IVO69_002718"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IVO69_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7401441.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          444 a          323 c          354 g          551 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc ttttcttagt
     1321 agttagattc taccaaatcg tgtccatgaa atcatcctaa catcacattg aagagtccat
     1381 ttcactcaca gagtgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtga
     1441 ttgtccagga ctcttttttt caatgaattg ttttccgcca actcattgag tggtgcgatt
     1501 tgcgactaca ttatatcaaa agaaagagaa gggaggaagc agaaatggtt cattcaacga
     1561 ttggagttgt tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa attcaaacag
     1621 gcagatcttt tgtcgtcaca gacaaagcgg caaaagctta tcaaagtagc ga
//
LOCUS       DACCPY010000171         1615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCPY010000171 DACCPY010000000
VERSION     DACCPY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.184"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            689..1021
                     /locus_tag="IVO69_002719"
     CDS             689..1021
                     /locus_tag="IVO69_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7401442.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          533 a          235 c          326 g          521 t
ORIGIN      
        1 tttaaattca taatcgttag atattttagt tatttttcac tcaaaaaaat agggaactag
       61 tgtttgcata tgtgcttttt tgagttataa taattcatga agagaggtaa ttcacttatt
      121 tccccaagct gaaaagcctg atattacttc ttttcaggca gtgctgctaa tattcaatta
      181 cacttttttg tatattttta gttgttttaa atttaattgt ctgctatgct tgtgatgaat
      241 tgattttcgt tctgcacttt tggacgataa atttggacaa ttgaaaatac aaaaaaaaga
      301 aaggccctgt gagactcgga aggctggtaa ccttcgtcga cgtccacagg tggcattaag
      361 atcactccct taatgcgttt acctttcagt gttacggtat tacccgtaac ggatttagta
      421 taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa aaatacgtga
      481 tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg gctctttgcc
      541 gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct cttttttgtt
      601 tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa
      661 tatttaaagt aagtgggtgg attattttat gtcatataca ggaattttga gtctcgaaga
      721 tatttgtcac tacggtaaac ggtgtaccgc tacagaaaag atcacgaaga aactatcaac
      781 tggtcaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc
      841 agaagaaatg atttattaca ttgggaaaca aaaacaaata attttaaaag atcctatccc
      901 tttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg
      961 tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt taatcagctg
     1021 aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga
     1081 gacttctaaa ggaacttaga atcactagaa aaataaggag tagacttgat ctatcaacaa
     1141 ttcctttcgg ggggtgacgg ggtaggtttt tacgattggt aacgtaaaaa attggtcaat
     1201 cgagtaaatg cccttgtttg gatagtggtg aaatgaattt gtgtaaataa tatgcacaga
     1261 gtaaacaaga gggaatttag aagttagaca agttagaata gcgcctagat taatcatgag
     1321 cagctactaa gggatctagg ctttgacgca aactcatgga ataaggagta ggtcttctcg
     1381 taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga
     1441 agacgttgat ctttggatca atcgagcatt taccgttttc tactagggtt tctcgtttcg
     1501 gacgccataa acgagtcgat ttattttata tatttttgag tgaacgaaaa acgataaaaa
     1561 tattcgttat gaagtaaaac ggttctgttg caaagtttta aataaagaat aaaat
//
LOCUS       DACCPY010000172         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCPY010000172 DACCPY010000000
VERSION     DACCPY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.185"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            138..1001
                     /locus_tag="IVO69_002720"
     CDS             138..1001
                     /locus_tag="IVO69_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7401443.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            1106..>1564
                     /locus_tag="IVO69_002721"
     CDS             1106..>1564
                     /locus_tag="IVO69_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7401444.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKR"
BASE COUNT          559 a          245 c          315 g          445 t
ORIGIN      
        1 gggagtatga cacaactgtt gtcatactcc ttttttcacc tttccaaacc aaggctcagc
       61 tattcaagta gataacatta tgttatgata gcaaagaaat gtattaagaa aacctttcac
      121 tatagaggga gaaaaaaatg aacgatttat tagcaactat atttacaggt attattattg
      181 atgaaaatga aaaaaactac tttgtccaaa aaaacggaca aacctttggc ttaagcaaat
      241 cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt cggttaccaa aatcaaaaac
      301 atgaaaatcg gatcactaca caaattccta aaagccgtat cggtcattac gcttttggaa
      361 tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga tatcggtctt ccagataaag
      421 actttgtcgt atctcttgat gaattgccga caatgacaga gctatggcct aaaaaagggg
      481 atcgcttgat gatttccctt agagttgatg caaaagaccg tatttggggc agtttagcag
      541 atgaaaaaat attcaaatct ttaagcaaat atggaacaga agaaatgaaa aacaaagatg
      601 tgataggaac agcataccgc ttgaaattaa caggtacgta cgttttaacg gaagatttct
      661 atcttgggtt tatccatcct tctgaacgat ataatgaacc tcgtctaggc gagcagataa
      721 aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa tctttcattg aagccaagag
      781 catatgaagc tatatcagat gatgcacaaa tgattttaac cattttagaa cgttctgcag
      841 atcagcaaat caactttaca gataaatcgg atccagaaga aatcatgaaa gcttttggta
      901 tcagcaaagg agcatttaag cgcgcattag gtaatctttt gaaacaagga ttgattacac
      961 aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg agaatccctt gcatgttttt
     1021 cttaactagc ttataataat tttaattaac atagtgttat atcttttttt atttgtaatt
     1081 attataaatt gttggggttg agtaaatggt catgaatgct atttcggcga tgaaaaaaac
     1141 aaaacagcag ctacatgaag cggggtttaa gctgacaccg caaagagaag ctactctgct
     1201 tgtgcttttg gaaaatgaga aagagcattt atctgcagaa gaaatcttct ttttagtgaa
     1261 aaaaaagagt ccggaaattg gacttgctac tgtttatcga acccttgaaa tcttgacaga
     1321 attgaaaatt gtcgataaag taagttttaa tgatggatta gcccgttatg atttgagaaa
     1381 agaaggcgcc aaacatttcc atcatcattt actatgcttg aactgcggca cgatcgagga
     1441 agtagaagag gatcttttac ttgaagtgga gcaagtagta gaagaacgtt atcatttttt
     1501 agtaaaagat catcggctaa cgttccatgg aatctgtcag gaatgctatg aaaaagaaaa
     1561 aaga
//
LOCUS       DACCPY010000173         1514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCPY010000173 DACCPY010000000
VERSION     DACCPY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.186"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..238
                     /locus_tag="IVO69_002722"
     CDS             <1..238
                     /locus_tag="IVO69_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP7401445.1"
                     /translation="QILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRE
                     LFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            258..1478
                     /locus_tag="IVO69_002723"
     CDS             258..1478
                     /locus_tag="IVO69_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7401446.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          525 a          273 c          305 g          411 t
ORIGIN      
        1 ccaaatattg gattcaccag agttaaaggc acgaattatg ccaagctcag aatttttatt
       61 gggtcattca ctccaagcct ttgatgagca aggacgactc attgatgaag aacaagtcgc
      121 aaaattacgt gaactattca aagatttctt actctttgtc acaatcacta gtcaattgca
      181 acatgcgcac caagcaaata aacgtgaagc cgaaagcttt tcatgggaaa cgatttaatc
      241 gaggggagca aacaaagatg aaattaatag gtatcgtcgg atcagcagct gaactttcat
      301 ataaccgtaa attattaaca tttatccaaa aacaatatca tgaactattt aatttagaaa
      361 tcattgaaat caaagattta cctctgttca atcaagatga tgatcaaaca aatagtcctg
      421 ctattcaaca gctaaatcgc aaaatcttaa tggctgatgg ggtaattatt gctacgccag
      481 aacacaatca tacggttcca gcaggattga aaagtgcact tgaatggcta tcatttaaaa
      541 tccatccact tgaaaataaa cctgtaatgg ttgtgggtgc ttcttactac gatcaaggaa
      601 cttcacgcgc gcaattacac ttgcgccaaa ttttggacgc accaggcgtg aatgcattag
      661 tgatgcccgg aaatgaattt ttattgggaa aagcaaaaga agcatttgac caagaagatc
      721 gcttgattga tccacgaact gttgactttt taggcagtac attagcaagt tttgtaaaat
      781 tcattaaagt cgtgtctacc ttagcagggc ctaaaaagct tccaccagaa gatttgcatg
      841 cgacaggaac aattgctacg acaatacccg atgttgatat gtcagatgat cattggttgg
      901 aaaaagcttc tgaagcagtc ggtgccgttt ctggaaatac ttacgtcaaa ttaaatcgtg
      961 ggttattgac agtggatcaa atcaattact ttttagattc gatgccgatg gaattaactt
     1021 ttgcagataa taataaccaa tttttatatt ataatttgcg ccaagaagcg aaagagatgt
     1081 tagctgctcg tgtgcccggt caggtaggga atccattagc caactgtcac ccagaaaaat
     1141 cgtataaaaa tgtagagtgg gtaattcaac aactgcgaag cggagcaaca gatgcggtac
     1201 gtgttcatgt accaaaacaa gtgccagaaa aatatattgt gcataattac caagcaatgc
     1261 atgatgaaaa tggagagtat gctggaatca atgaatacat tcttgatttc aaaccaatca
     1321 ttgagtggta tttacaacaa acgggtcaac aacttgttgg aaatgaagat gcggtttcaa
     1381 gtgcatctgt tcatggtgga aaaacagatg cagtaaccag cgccagcaca cacgatgaaa
     1441 ctgcagtaga tgcagtttcg agtgcttctg taaaataaaa aggttctgtt gcaaagtttt
     1501 aaataaagaa taaa
//
LOCUS       DACCPY010000174         1469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCPY010000174 DACCPY010000000
VERSION     DACCPY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.187"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IVO69_002724"
     CDS             <1..365
                     /locus_tag="IVO69_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7401447.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IVO69_002725"
     CDS             366..914
                     /locus_tag="IVO69_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401448.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1174..1469
                     /locus_tag="IVO69_002726"
                     /pseudo
     CDS             1174..1469
                     /locus_tag="IVO69_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          577 a          185 c          313 g          394 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac ctttaaacgg tagttactta
      961 gttcttcttt gatttgcatg aaatagaggg gatttatttt tctggttacg tgattaaaaa
     1021 atccaaaaaa tgagcatagg aacggttatg attttagggt gtgttttaat gagatattgt
     1081 gatttcatta acaagtgtat gataatctgt tgttgagtta gtaaataagt aatagagcta
     1141 actctaaaat aaaactatta ggaggagatg taggtgaaaa agtttaaaaa gaaaatatat
     1201 ttaggattgg ttgttggggt gagtatgttt gggttcatgg ctcatactaa acaagtaact
     1261 gcagatacta accaaaaaga aaggattatg agccaattag atgactctca aataagtatg
     1321 tacccaatga gctaattgtc agtttaaagg ctacacaaag aatttcaaca gagaaaaata
     1381 aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa agatttgact cttaggacaa
     1441 atgaaagcga ttgtaataag cgagatcaa
//
LOCUS       DACCPY010000175         1460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCPY010000175 DACCPY010000000
VERSION     DACCPY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.188"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            74..484
                     /locus_tag="IVO69_002727"
     CDS             74..484
                     /locus_tag="IVO69_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7401449.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            498..854
                     /locus_tag="IVO69_002728"
     CDS             498..854
                     /locus_tag="IVO69_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7401450.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          269 g          440 t
ORIGIN      
        1 tttattagct aataattggt attttaaaag aaattactgt agaattggat tataattaaa
       61 aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
      121 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      181 gttaacagaa agtgagatca aaaagatctt agcagcgact gaaggaggag ttcaagatat
      241 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      301 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      361 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      421 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      481 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      541 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      601 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      661 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      721 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      781 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      841 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      901 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      961 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
     1021 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1081 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1141 atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa
     1201 aaacttcaaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
     1261 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
     1321 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat
     1381 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatttgtagg gcgtcttgat
     1441 tcaattcaag acaacaaaca
//
LOCUS       DACCPY010000176         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCPY010000176 DACCPY010000000
VERSION     DACCPY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.189"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IVO69_002729"
     CDS             108..1040
                     /locus_tag="IVO69_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP7401451.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="IVO69_002730"
     CDS             1112..>1455
                     /locus_tag="IVO69_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401452.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DACCPY010000177         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCPY010000177 DACCPY010000000
VERSION     DACCPY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.190"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVO69_002731"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVO69_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7401453.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          239 c          213 g          511 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaat
//
LOCUS       DACCPY010000178         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCPY010000178 DACCPY010000000
VERSION     DACCPY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.191"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1337
                     /locus_tag="IVO69_002732"
     CDS             <1..>1337
                     /locus_tag="IVO69_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401454.1"
                     /translation="PENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEH
                     ENTVEPTSLENKIYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHI
                     QKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKE
                     FPNQPIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLA
                     DIERYNGDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAG
                     LIQAFSRTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESK
                     KRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQS
                     STLNNQIQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDS"
BASE COUNT          481 a          237 c          282 g          337 t
ORIGIN      
        1 cctgagaatc ggaaacaagc caaagggcaa ttggcacgta cgacccatga tcagtatgga
       61 gaggtcttgc acacctatac catcaagaat gcattagaag atggctcggt tttaggattt
      121 caagtagagc atgaaaacac cgtggagccg acttcattag agaataaaat ctatcggaaa
      181 ttaaaagaag tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat
      241 caaatggagt ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa
      301 catatccaaa aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat
      361 tttcgaaatg gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc
      421 aaaaagtatt atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag
      481 tttcccaatc agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc
      541 gctattacct attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg
      601 caaaaaatta ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt
      661 gagcgttata acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa
      721 tttgggaaac aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc
      781 cctacgattc aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc
      841 ttttcacgta cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga
      901 aagccagcga caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa
      961 gaagaatcgg gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca
     1021 tatggtaagt taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa
     1081 atccgtatcg agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg
     1141 acttatgatg attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa
     1201 cttttggaag aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa
     1261 ccagaaaaag agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag
     1321 ttgtatgata ttgattc
//
LOCUS       DACCPY010000179         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCPY010000179 DACCPY010000000
VERSION     DACCPY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.192"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1337
                     /locus_tag="IVO69_002733"
     rRNA            <1..>1337
                     /locus_tag="IVO69_002733"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACCPY010000180         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCPY010000180 DACCPY010000000
VERSION     DACCPY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.193"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            44..>1328
                     /locus_tag="IVO69_002734"
     CDS             44..>1328
                     /locus_tag="IVO69_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7401455.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          452 a          259 c          252 g          365 t
ORIGIN      
        1 ttaaatcacc acaaaataaa caactgagag gacgatacgc tatatgaatg attctatcaa
       61 aaaaatgctg agaataacag agaaagattt gatgattaca gaggtctctt acgagaccct
      121 tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag
      181 aagttgtggt tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatggaaa
      241 aaaggaaacg attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa
      301 aaagcaacgc tatacctgta aaaactgtcg cacccattgg acggctcaaa gttattttgt
      361 ccaacccaga cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga
      421 aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg
      481 tacattgaaa gagtttaaaa gctatttacc aaagcaatct aagaagatcc tccccagagt
      541 attgatggtt gacgaatttc gttcgcatgc ttccatagaa gataagatga gctttatttg
      601 cgcggatggc gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt
      661 aacaagctat ttcttaggtt gtaccaatcc agaagaagta gaattcttgg tgacagacat
      721 gaacgccgcc tacttccagc tcaccaaacg tgttctgcca aatgcgaaag tcgtgattga
      781 tcggtttcat attgtcaaac acatgaatca agcgttcaat gagttgcgta tccgtgaaat
      841 gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa agaactggcg
      901 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      961 ccgagcacca aaatacccat ttcttactga agcaatgatg attgatcgat tgcttgagtt
     1021 ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag cctttcgaga
     1081 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacag
     1141 ctttcgggaa aagcttcaaa accttctgac ctatgaagaa ggcatcacca acgcaatgat
     1201 ctatccttat tccaatggaa aaatagaagc gaagaatacc cacataaaga caatgaaacg
     1261 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
     1321 attaatca
//
LOCUS       DACCPY010000181         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCPY010000181 DACCPY010000000
VERSION     DACCPY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.194"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IVO69_002735"
     CDS             414..947
                     /locus_tag="IVO69_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7401456.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          416 a          239 c          244 g          403 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc ta
//
LOCUS       DACCPY010000182         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCPY010000182 DACCPY010000000
VERSION     DACCPY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.195"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IVO69_002736"
     CDS             200..742
                     /locus_tag="IVO69_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401457.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVO69_002737"
     CDS             832..>1246
                     /locus_tag="IVO69_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7401458.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCPY010000183         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCPY010000183 DACCPY010000000
VERSION     DACCPY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.196"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            34..531
                     /locus_tag="IVO69_002738"
     CDS             34..531
                     /locus_tag="IVO69_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401459.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(568..1185)
                     /locus_tag="IVO69_002739"
     CDS             complement(568..1185)
                     /locus_tag="IVO69_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401460.1"
                     /translation="MYSQTIDSLNKRFGNRVLKGIEIGYIASEKDRIIDYLADKDYDL
                     KLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGL
                     SVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFS
                     VGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVPLPV"
BASE COUNT          430 a          208 c          216 g          380 t
ORIGIN      
        1 tttatagtat actatgtcaa aaggaggagt tagatgaaca atcaaatcgc actagcgtat
       61 ttagattatg atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg
      121 tttatcgatg gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact
      181 gaaatattgg cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat
      241 caaatactca ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg
      301 ctctatctgc tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg
      361 aatcgttatg ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat
      421 catgcaggta ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttt
      481 cctttaggaa aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc
      541 ctcacttttg atgctgagga cgttttttta gactggtaat ggaaccataa ttcgctttct
      601 tttttgataa atcgctaatt ccgtcacacc ttttgctttt aacagagcaa aaagatcatc
      661 gaaatggaaa cggaaatgtt caaggtaatg cccatctgat ccaacactaa acaaggtacc
      721 accacgtgaa agatataaat cgatgacata ttcatataac gctaaattgt catacaagta
      781 agcgctctta gcatttaatt cgaatgccaa tttatttttg attacttgat ctagaatcgg
      841 taaaaactgc gcttcatatt gtttaaattc agcaacggat agaccaaata cccgaaaacc
      901 atagtcaaaa tgagcaaaga catctgcttc tataacaact agtgcttctg aaagttgtgc
      961 aaaatactga ggaatcacaa tagcaggatc catatctttg acttcgtcat ctaaataatc
     1021 aaattgacca ttatgatgca cactgagtaa ttttaaatcg taatctttat cggctaaata
     1081 gtcgatgatc cgatcctttt cagaagcgat gtaaccgatt tctatgcctt ttaaaacacg
     1141 attgccaaaa cgcttattta agctatcgat tgtttgactg tacatttcat aatcggggat
     1201 atcatcgact ttagtaacag gattggctaa gtca
//
LOCUS       DACCPY010000184         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCPY010000184 DACCPY010000000
VERSION     DACCPY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.197"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IVO69_002740"
     CDS             141..398
                     /locus_tag="IVO69_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401461.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVO69_002741"
     CDS             complement(630..1043)
                     /locus_tag="IVO69_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7401462.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACCPY010000185         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCPY010000185 DACCPY010000000
VERSION     DACCPY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.198"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..139
                     /locus_tag="IVO69_002742"
     CDS             <1..139
                     /locus_tag="IVO69_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7401463.1"
                     /translation="RRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNN
                     I"
     gene            387..1121
                     /gene="erm"
                     /locus_tag="IVO69_002743"
     CDS             387..1121
                     /gene="erm"
                     /locus_tag="IVO69_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP7401464.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYTKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          449 a          173 c          179 g          355 t
ORIGIN      
        1 ccggagagaa cgagtgattc ggatttttcc aaatatcaat tccatcactc ggataattgg
       61 aacacttttg atggaaaaag acactgagtg gctggcttct ccacgaaaat atttagaatt
      121 taattcgaat aacatttagc accatgaacc tgtgcgagtc tcttctttct gaggagagac
      181 atcaagagtc gctgcgctac tgctcctgac ctctctcctc agaaagaaag gcatggcagt
      241 taggttcaca acgcaaaaca ttgtgctttt gatgaggttg aacttttaca caagattgtg
      301 gacttgactc aaccaaacaa aaaataagtg gttataatgg attgttaata taaacattca
      361 ttataacctc ataggagtgg gttataatga acaaaaaaaa tataaaagat agtcaaaact
      421 ttattacttc aaagcatcat ataaatgaaa ttttgagaaa tgtacattta aatacaaatg
      481 ataatattat tgagattggt tcagggaaag gtcatttcac gtttgaatta gctaaaaggt
      541 gtaattatgt aaccgccatt gaaatagatc ctaaattatg taggataact aaaaacaaac
      601 ttattgaata tgagaacttc caggttatca ataaagatat tttacaattt aagtttccta
      661 aaaataagtc atataagata tatggaaata taccctacaa tataagtaca gatataattc
      721 gaaaaattgt ttttgaaagc acagctacag aaagttattt aatagtggaa tatggatttg
      781 ctaaaaggtt gctaaataca aatcgttcac tagcactatt tttaatgaca gaagttgata
      841 tatccatatt aagtaaaatc cctagagaat actttcatcc aaaacctaga gttaatagct
      901 cgttaattgt attaaaaaga cacccttcaa aaatatcact caaagataga aaacaatatg
      961 aaaattttgt tatgaaatgg gttaacaaag aatacacaaa actattttcc aaaaaccaat
     1021 tttatcaagc cttaaaatat gcaagaattg acgatttaaa caatattagc tttgaacaat
     1081 tcttgtctct tttcaatagc tataaattat ttaatagata agttaagggg tgcataaact
     1141 gcatccctta acttat
//
LOCUS       DACCPY010000186         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCPY010000186 DACCPY010000000
VERSION     DACCPY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.199"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(251..1132)
                     /gene="galU"
                     /locus_tag="IVO69_002744"
     CDS             complement(251..1132)
                     /gene="galU"
                     /locus_tag="IVO69_002744"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7401465.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          287 a          255 c          179 g          413 t
ORIGIN      
        1 actatgaatc ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac
       61 ttaaaaaaaa attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt
      121 ctatctttcg ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc
      181 gcaaatgacg cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac
      241 ttatttccag ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta
      301 atgaagtccc aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta
      361 acttatcgcc tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct
      421 ggttaagagt ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag
      481 tttctaatat ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac
      541 ttggtgcttt tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt
      601 cgctatctgt tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca
      661 tgacggcaac agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg
      721 ggatatcatc tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa
      781 atgcttttgc ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt
      841 gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc
      901 gcaaattgtt ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt
      961 ttcccgttac gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact
     1021 ggatcgttgg tttatccacg attggaagca tttcttttgc catggctttt gttgccggca
     1081 aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt tttgaccttc actt
//
LOCUS       DACCPY010000187         1114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCPY010000187 DACCPY010000000
VERSION     DACCPY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.200"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1027)
                     /locus_tag="IVO69_002745"
     CDS             complement(<1..1027)
                     /locus_tag="IVO69_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7401466.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVL"
BASE COUNT          328 a          241 c          186 g          359 t
ORIGIN      
        1 ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa
       61 tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac
      121 catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa
      181 agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa
      241 tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa
      301 cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc
      361 gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg tcttgaacat
      421 caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata tatacacctt
      481 ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac atgaaaaatg
      541 gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga tccaactgtt
      601 tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc agttcaactg
      661 ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg gcagcgatca
      721 aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta ccgtctctgg
      781 ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga tcgtagtaac
      841 catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga atatattgat
      901 ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt gcggtatcat
      961 tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta aaatgtactt
     1021 gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg tactcctaac
     1081 tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCPY010000188         1111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCPY010000188 DACCPY010000000
VERSION     DACCPY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.201"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            6..398
                     /locus_tag="IVO69_002746"
     CDS             6..398
                     /locus_tag="IVO69_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401467.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>1111
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002747"
     CDS             399..>1111
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP7401468.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYA"
BASE COUNT          450 a           86 c          197 g          378 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg c
//
LOCUS       DACCPY010000189         1109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCPY010000189 DACCPY010000000
VERSION     DACCPY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.202"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            56..235
                     /locus_tag="IVO69_002748"
                     /pseudo
     CDS             56..235
                     /locus_tag="IVO69_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            297..481
                     /locus_tag="IVO69_002749"
                     /pseudo
     CDS             297..481
                     /locus_tag="IVO69_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            515..946
                     /locus_tag="IVO69_002750"
     CDS             515..946
                     /locus_tag="IVO69_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP7401469.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          377 a          167 c          232 g          333 t
ORIGIN      
        1 gatgattgca gccatatttt tcataggatt atttcgatga aaggagagag taccaatgcc
       61 aattgcaata ggaaataaac ggttacccgt gacgttggat gaaaagagac aaaaagaatt
      121 gcagtaacca aagcagaagt acggcaaaag tgaatccagg attatgtgta ttgcgttaga
      181 tttattgatt gcgcaagaaa aagcaggatt tgacatacca gcactcaaaa agtgatcgca
      241 ctttgcgcac gtgttaatac gtgttataat tattttgtaa ggaggtatag tgcacaatgc
      301 cacttacggg aaaagaaatg cttaagttat tgaaaaaaat ggctgggttg agcgaaggca
      361 agagggattt catcatcatc tttataagga tggtgttaga atcactgttc ctgttcatgc
      421 caatcaagat cttggccgag gacttgagcg aaaaatttta aaggatgcgg gattgaaata
      481 atctcgcatt cgattaaaga aaggattgac tgaaatgtta agtgagcctt tagcaaaaac
      541 ctatcctgct atttttagcc cagaagaagg tggtgggtac tttattgaat ttcctgatgt
      601 tcaaggtgct tacacaggaa ttaacgaaga cgatatatct tatggaattg ctatggcaca
      661 agaggtgtta ggtatggttt tagcagatta tattgagcat gaagatctac ttccagaacc
      721 aacaccaatc aataagattt ctgttgaaga tgattctttt actactttaa ttcgtgtaga
      781 tgttgccaaa tatttgaaag acacagaact tgtcaaaaaa accttaacaa ttcctcaatg
      841 ggcggataag ctaggcaaaa gggctggcat aaatttttca gtccttctaa cagaatcaat
      901 tgcagataaa gcagataaca ttcttcgttc tggaagaaat aattagaact caaaagggtc
      961 agaaacttaa aaatctttgg cttctttttt tactacaaag atggcttctt taaattattt
     1021 gcctgattga tttaaaaaaa ttgcgcaaag actacgagct aattaagttg ctcgtggtct
     1081 ttttttatga aaacttttta gatcttatt
//
LOCUS       DACCPY010000190         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCPY010000190 DACCPY010000000
VERSION     DACCPY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.203"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            59..913
                     /locus_tag="IVO69_002751"
     CDS             59..913
                     /locus_tag="IVO69_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7401470.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
BASE COUNT          418 a          143 c          181 g          349 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac a
//
LOCUS       DACCPY010000191         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCPY010000191 DACCPY010000000
VERSION     DACCPY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.204"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IVO69_002752"
     CDS             4..978
                     /locus_tag="IVO69_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7401471.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          382 a          135 c          187 g          385 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaa
//
LOCUS       DACCPY010000192         1080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCPY010000192 DACCPY010000000
VERSION     DACCPY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.205"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            122..385
                     /locus_tag="IVO69_002753"
     CDS             122..385
                     /locus_tag="IVO69_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7401472.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            385..657
                     /locus_tag="IVO69_002754"
     CDS             385..657
                     /locus_tag="IVO69_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7401473.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            734..1063
                     /locus_tag="IVO69_002755"
     CDS             734..1063
                     /locus_tag="IVO69_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7401474.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          410 a          134 c          214 g          322 t
ORIGIN      
        1 taaaggaaac ggataacact ttacaatgct gttttggtac tccaatttaa acaaaacaat
       61 cgaatttacg tacttaatta agtacgttag aatggagata taaattagag ggggagtaga
      121 tatggcaaaa acagtcttaa atcctagctc agcacggaaa aatttttata agttgttgaa
      181 agatgtaaat gagaatcata cggaaattga aatcattagt gagcgcagtg aaaataatgc
      241 ggtgcttatt ggtttggatg attggaaagc aatcaaagaa acgctattac ttgagcaaat
      301 aggcactttg ggtgttgttc gtaagcgaga aaaagatgat agtggattta ctaatgttga
      361 tgaaattgat tgggataact tataatgaat aactattctg tgatgattaa aaactcggct
      421 aaagctgatt taaagaaaat caaacagtct aatttgaaac ctcaatttga gaaggttatt
      481 cagactttaa aggaagatcc ctatctccct acccaatcat ttgagaagtt aaaacctaca
      541 catgaaggta ggtattctag aagattgaat agacaacacc gggttgttta taaggtagat
      601 gaagatgaac acgtggttga aatttattca gcaaggacgc attacgagtc acagtaatta
      661 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
      721 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      781 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      841 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      901 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      961 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
     1021 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DACCPY010000193         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCPY010000193 DACCPY010000000
VERSION     DACCPY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.206"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            2..>1073
                     /locus_tag="IVO69_002756"
     rRNA            2..>1073
                     /locus_tag="IVO69_002756"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACCPY010000194         1065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCPY010000194 DACCPY010000000
VERSION     DACCPY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.207"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1065)
                     /locus_tag="IVO69_002757"
     CDS             complement(<1..>1065)
                     /locus_tag="IVO69_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP7401475.1"
                     /translation="FEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMT
                     HDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGERE
                     KQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKT
                     PKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFSTVTRE
                     GYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKK
                     RKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFF
                     CSKIRNEVDKKLFQARVTSL"
BASE COUNT          287 a          222 c          189 g          367 t
ORIGIN      
        1 ccaaactcgt aactcgagct tggaacagct ttttatctac ttcgtttcga atttttgaac
       61 aaaaaaataa aggctccttg cctttccata gccaggtggc taatttcttt aaattcattg
      121 cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa gcctcgatac ttggtccaac
      181 gcatcccatg cttttctttt gcatccgcaa atattcgttc aatcgtttgt ttccgttttt
      241 tgtaaattga tttattcaga tctgttaggc gaagatgttc cacttcatcc atcagatctc
      301 cccaaacatg tctcgtgatg actcgttgat gatttttact tatggtacat tggtttagca
      361 gaggacagtt cacacattct tttggattgg atttgtattc tcggtaccct tctcgagtaa
      421 ccgttgaaaa agcgagcatt tgatcttgcg gacaaatata acaatcaaaa tactcgtcat
      481 agagaaactc attttttgat aatagctcct ttttcccttt gggccttgaa taaggcaaac
      541 aaggtcgaag gttcaaatga gtcaaaaagt gaacaaactt aggtgtctta tacccagcat
      601 cagcgacaac acaattgatt tcaggaaaaa gtctcttcga ttgtttgacg agctgaaagg
      661 cgacttgact gtcatgaata tttctaggag tgatgattgt gctaagaata aatccattgt
      721 cgtcgcatga agtatggaca gaataagcaa attgtttttc tcgttcgcct ttgacgtagt
      781 agccgctttc cggatcagct ttacttattt ttctttcttt cagcttcact tccttttcta
      841 agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc tcatcttcta
      901 attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac ttatgtttat
      961 tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt acaaaacctg
     1021 ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaaca
//
LOCUS       DACCPY010000195         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCPY010000195 DACCPY010000000
VERSION     DACCPY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.208"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            628..969
                     /locus_tag="IVO69_002758"
     CDS             628..969
                     /locus_tag="IVO69_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7401476.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          372 a          130 c          246 g          314 t
ORIGIN      
        1 atcggctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
       61 attgtcgaga cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct
      121 atcaacaatt cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat
      181 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
      241 agcgcagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta
      301 atcatgagca gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg
      361 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
      421 acttgcgagg acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc
      481 tcgtttcgga cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac
      541 gataaaatat gaacaagtcc tgaataaagt gaaaaaatta atataaagca tagagaaaat
      601 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
      661 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
      721 aggggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
      781 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
      841 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
      901 tggtcatatt tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa
      961 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga
     1021 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga at
//
LOCUS       DACCPY010000196         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCPY010000196 DACCPY010000000
VERSION     DACCPY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.209"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IVO69_002759"
     CDS             complement(36..992)
                     /locus_tag="IVO69_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401477.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACCPY010000197         1038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCPY010000197 DACCPY010000000
VERSION     DACCPY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.212"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1038
                     /locus_tag="IVO69_002760"
     CDS             <1..>1038
                     /locus_tag="IVO69_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7401478.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKH"
BASE COUNT          361 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacacc
//
LOCUS       DACCPY010000198         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCPY010000198 DACCPY010000000
VERSION     DACCPY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.213"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IVO69_002761"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVO69_002761"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCPY010000199         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCPY010000199 DACCPY010000000
VERSION     DACCPY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.214"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            436..>1025
                     /locus_tag="IVO69_002762"
     CDS             436..>1025
                     /locus_tag="IVO69_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401479.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGR"
BASE COUNT          361 a          172 c          192 g          300 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaattaatca atctgttgct aaaactgccc attgcttgaa tcgttcaaga
      541 caaacgatcc ataaagtata tctgttcttc aagcaaggaa aatcagcctt agaatattat
      601 caacagtata agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa
      661 caatcagaat atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt
      721 cgtgcagcgt ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg
      781 ctatttgatt cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa
      841 agacgtggaa aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa
      901 ttctcaaatg agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt
      961 gctgtaatta ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt
     1021 agaca
//
LOCUS       DACCPY010000200         1004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCPY010000200 DACCPY010000000
VERSION     DACCPY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.215"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            345..524
                     /locus_tag="IVO69_002763"
                     /pseudo
     CDS             345..524
                     /locus_tag="IVO69_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            583..936
                     /locus_tag="IVO69_002764"
                     /pseudo
     CDS             583..936
                     /locus_tag="IVO69_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          358 a          143 c          190 g          313 t
ORIGIN      
        1 aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcgc tccaaccgcc
       61 cccgccgctt aggtggtgcg aaataagcga acaaagcccg aaaacctaag agaaagaagg
      121 agatttatat gaacaaaatt attcccttat tattagtcct atttgttgga gaaatactta
      181 tgcgacttag tgcagctgta catttaaacc tcgcagtatt tttggtaata ctaatgtttt
      241 gtttgatctt ttggcgattt gtgttagccc ttgtgagtct aatattcaag ttgattgcag
      301 ctatattttt catagggtta tttcgatgaa aggagagagt accaatgcca attgcaatag
      361 gaaataaacg gttacccgtg acgttggatg aaaagagaca aaaagaattg cagtaaccaa
      421 agcagaagta cggcaaaagt gaatccagga ttatgtgtat tgcgttagat ctattgatcg
      481 cgcaagaaaa agcaggattt gatgtaccag cactaaaaaa gtgacgtcac tttttaactt
      541 gatccgtata agtgatatca cttttagcaa ggagggaaat aaatgttaaa aactaaaaag
      601 aattcgtaca aattaaagct tagtatacta tctattattt gtcttgtaga tatgataatt
      661 agtggtcatt tgtatagagt ggcatatacg cagtttattg agtacaaggt tcactattta
      721 ggattagctg ggaatcgata cgttgaatat gtccattatt cattaaaatg ttcttcgtat
      781 attcttcttt ggcttatttt ttcattgttt gaattgctaa atttaatagc aatatcttca
      841 tatcacttag ataagctaaa caccaaatct agccgttata acgaagtgtt caaggaaaat
      901 atgaagttag aaagtgactt aaacaagcta aaaaaaataa aaagaagaaa acataagtta
      961 actaaaatta ttttattaag aatgatggaa tataaggata aggc
//
LOCUS       DACCPY010000201          971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCPY010000201 DACCPY010000000
VERSION     DACCPY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.216"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(717..962)
                     /locus_tag="IVO69_002765"
     CDS             complement(717..962)
                     /locus_tag="IVO69_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP7401480.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          315 a          184 c          137 g          335 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca cttgactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tctcaagtgg aatcaactca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatgtt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg tagatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac t
//
LOCUS       DACCPY010000202          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCPY010000202 DACCPY010000000
VERSION     DACCPY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.217"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>947
                     /locus_tag="IVO69_002766"
     CDS             <1..>947
                     /locus_tag="IVO69_002766"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7401481.1"
                     /translation="DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEV
                     IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQK
                     ERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAE
                     EMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSK
                     ADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLF
                     FGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKL"
BASE COUNT          303 a          170 c          209 g          265 t
ORIGIN      
        1 acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat
       61 ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga
      121 cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac gatggggacg
      181 tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca gggaagaaag
      241 ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc gtaacgctcg
      301 atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca ggttcattga
      361 tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat gggcaagaac
      421 taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt gtcgacgccg
      481 aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca gatgaacctt
      541 acacatttga tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga
      601 ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa tccaaagcag
      661 attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc atggccatcg
      721 tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt caaaaattac
      781 tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc tttgggacat
      841 cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat gtattattca
      901 ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaacta
//
LOCUS       DACCPY010000203          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCPY010000203 DACCPY010000000
VERSION     DACCPY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.220"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IVO69_002767"
     CDS             <1..861
                     /locus_tag="IVO69_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7401482.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACCPY010000204          829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCPY010000204 DACCPY010000000
VERSION     DACCPY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.221"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            12..>829
                     /locus_tag="IVO69_002768"
     CDS             12..>829
                     /locus_tag="IVO69_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401483.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT          281 a          146 c          178 g          224 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagt
//
LOCUS       DACCPY010000205          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCPY010000205 DACCPY010000000
VERSION     DACCPY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.222"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..780
                     /locus_tag="IVO69_002769"
     CDS             <1..780
                     /locus_tag="IVO69_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7401484.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          244 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccctt t
//
LOCUS       DACCPY010000206          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCPY010000206 DACCPY010000000
VERSION     DACCPY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.223"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IVO69_002770"
     CDS             complement(<1..461)
                     /locus_tag="IVO69_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7401485.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          224 a          128 c          101 g          252 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcga
//
LOCUS       DACCPY010000207          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCPY010000207 DACCPY010000000
VERSION     DACCPY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.224"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACCPY010000208          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCPY010000208 DACCPY010000000
VERSION     DACCPY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.225"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            3..650
                     /locus_tag="IVO69_002771"
                     /pseudo
     CDS             3..650
                     /locus_tag="IVO69_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          218 a          119 c          129 g          184 t
ORIGIN      
        1 taatgaatca ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt
       61 actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat gatcgtggaa
      121 taaatgtttg tcatactacg atttatcgtt gggtataaga gtacagcaaa gtcctctatt
      181 atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac gaaacctata
      241 tcaaaattaa gggacgttgg cattatcttt atcgtgcaat tgatgcggac ggcttaacct
      301 tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc ttaaaacgac
      361 tccataaaca gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct tctcttggct
      421 ccgcctttag aaagttacag agtgtgggtt tatatactaa gacagagcac cgaactgtga
      481 agtatcttaa caatttaata gaacaagacc atcgacctat taaacgacga aataaatttt
      541 atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt cgaggaatat
      601 ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
//
LOCUS       DACCPY010000209          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCPY010000209 DACCPY010000000
VERSION     DACCPY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.226"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..426)
                     /locus_tag="IVO69_002772"
     CDS             complement(<1..426)
                     /locus_tag="IVO69_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7401486.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQ"
     gene            complement(442..558)
                     /locus_tag="IVO69_002773"
     CDS             complement(442..558)
                     /locus_tag="IVO69_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7401487.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          193 a          123 c           90 g          204 t
ORIGIN      
        1 ctgataaacc gttgataaat caattccatt ttggtcaatc ttattgataa aaaagattgt
       61 gggaatcccc attttcctaa gtgcatgaaa taatatacga gtttgtgctt gtacgccatc
      121 ttttgcagaa atcagtagaa ttgccccatc taaaactgat aatgaacgat atacttctgc
      181 taagaaatcc atatgtcctg gcgtgtctat gatgttcacc ttcgtatttt cccactgaaa
      241 agaggttatt cctgtctgaa ttgtaattcc tctctgacgt tctaaaagcg tattatccgt
      301 cctcgttgta cctttgtcca cgcttcctaa ttctgtaatc gctccactgt tatataataa
      361 gctttctgtt aaggtagttt ttcctgcatc aacatgagct aaaactccaa tattaataat
      421 tttcatgtga ttttcctcca ttcaaaaacc caaaagggca taaaaatccc agtgataaat
      481 acttttatca ctgggatttt tatgcataac cataggtata caaagcatac agatattctc
      541 tggatacttt agaatcacat gataaaggta ttcttaaact gggtacaaaa aactaagccc
      601 tcctaaaaaa
//
LOCUS       DACCPY010000210          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCPY010000210 DACCPY010000000
VERSION     DACCPY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.227"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>609)
                     /locus_tag="IVO69_002774"
     CDS             complement(<1..>609)
                     /locus_tag="IVO69_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAP7401488.1"
                     /translation="FLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYST
                     MSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIE
                     KDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCD
                     FIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY"
BASE COUNT          210 a          103 c           43 g          253 t
ORIGIN      
        1 ataatattct tcaactatat cttgatattc ttttgctttc tcaatatcta tatttccata
       61 cattcttaat atatcttctc caaaatttgt tcctatttct tcttcactat cttcaagtaa
      121 gtatataaaa tcacaatatt catctataat tccagaatct ccaaaatcaa ttattccagt
      181 taatctatta ttgccatcta acaatagatg attacaacta aaatcattat ggcataaaca
      241 ctttttaccc tcaaaaactg ttgttgcatt tagtctttcc ataaaacttt ctatataatc
      301 tttttctata tcagttaaat cattataaat agtttcacgc aacaatatat actcttctaa
      361 tacattttgt ttattatcaa tagtacattc actaatatct gtataatcta aaccgtgcat
      421 ttgtcttaaa aaactggcaa tatctcgttt taacaaattt tgttcttctt ctgacatagt
      481 agaataaatt tctggtgtta aaaaagttcc tttaatttct ttataaccta gtatagataa
      541 ttcatcacta atatacgaat attcaatatt aggaattttt acattagttt ctaaatttgt
      601 atttaaaaa
//
LOCUS       DACCPY010000211          604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCPY010000211 DACCPY010000000
VERSION     DACCPY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.228"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(170..>604)
                     /locus_tag="IVO69_002775"
     CDS             complement(170..>604)
                     /locus_tag="IVO69_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401489.1"
                     /translation="GTYAANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLD
                     SNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINS
                     KDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          181 a          121 c           81 g          221 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcc
//
LOCUS       DACCPY010000212          588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCPY010000212 DACCPY010000000
VERSION     DACCPY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.229"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(65..150)
                     /locus_tag="IVO69_002776"
     tRNA            complement(65..150)
                     /locus_tag="IVO69_002776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IVO69_002777"
     tRNA            complement(167..238)
                     /locus_tag="IVO69_002777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IVO69_002778"
     tRNA            complement(250..322)
                     /locus_tag="IVO69_002778"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IVO69_002779"
     tRNA            complement(340..421)
                     /locus_tag="IVO69_002779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IVO69_002780"
     tRNA            complement(429..501)
                     /locus_tag="IVO69_002780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IVO69_002781"
     tRNA            complement(505..577)
                     /locus_tag="IVO69_002781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACCPY010000213          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCPY010000213 DACCPY010000000
VERSION     DACCPY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.230"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            70..>556
                     /locus_tag="IVO69_002782"
     CDS             70..>556
                     /locus_tag="IVO69_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7401490.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FM"
BASE COUNT          208 a          101 c           92 g          155 t
ORIGIN      
        1 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
       61 gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat
      121 ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa
      181 ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg
      241 agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata
      301 cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac
      361 gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt
      421 gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg
      481 actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat
      541 gtcgctattt ttatgg
//
LOCUS       DACCPY010000214          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCPY010000214 DACCPY010000000
VERSION     DACCPY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.231"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(120..299)
                     /locus_tag="IVO69_002783"
     CDS             complement(120..299)
                     /locus_tag="IVO69_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401491.1"
                     /translation="MVPACSAGGSGGSGRWYPSVRGVVPASHEHVSGTSEMWFQPARQ
                     VFPQCPPSGSGVVGR"
BASE COUNT          142 a          202 c          126 g           74 t
ORIGIN      
        1 tcgccagaac cacctgccga tcacgcaaaa atcacctgcc ggctacgcca caaccagttg
       61 gtgaacactt tggaatcacc tgccaggctt gctggaacca cctgccagtc atgccaaaat
      121 cacctgccaa ctacgccaga accgcttggc ggacactgcg gaaacacctg tcgggccggc
      181 tggaaccaca tctcagacgt gccagaaacg tgttcatgag atgctggaac cacaccccga
      241 acagacggat accacctgcc agaaccgcct gaaccacctg ctgagcacgc cggaaccacc
      301 tgctcgactc accggtacca cctccctgac acgccaaaat cacctgctgg gtatgccaga
      361 accacttggc ggacactccg gaatcatctg ccggacatgc cggaaccagc tctcagacac
      421 accagaacca cgtgccggag acgccggaag cacctgccga gcatgccgga gccacctgct
      481 cgactcgcca gaaacacctg ccggacacgc caaaatcacc tgccgggtac gccagaacca
      541 cctg
//
LOCUS       DACCPY010000215          540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCPY010000215 DACCPY010000000
VERSION     DACCPY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.232"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(44..>540)
                     /locus_tag="IVO69_002784"
     CDS             complement(44..>540)
                     /locus_tag="IVO69_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP7401492.1"
                     /translation="VRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPY
                     SLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFV
                     HQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTS
                     GEAA"
BASE COUNT          157 a          131 c           92 g          160 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcgaacat
//
LOCUS       DACCPY010000216          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCPY010000216 DACCPY010000000
VERSION     DACCPY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.233"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(66..287)
                     /locus_tag="IVO69_002785"
     CDS             complement(66..287)
                     /locus_tag="IVO69_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7401493.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          183 a           88 c          101 g          159 t
ORIGIN      
        1 tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt ctggtagcgt
       61 gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt ttttctatag
      121 tatataaatt tttgcatata gataaagatc aagataccga taacggaagc tagattgtaa
      181 tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga acctgcacca
      241 taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt gaagttattg
      301 atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata atccctgtag
      361 aaattagcat agtagctggg ataccaattc acatattgac aaagataaca gagactttta
      421 cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt ccaacaatgt
      481 gttaactggc ctagcgtcat tcagcaaatt atgcatgatg aggagcgaaa t
//
LOCUS       DACCPY010000217          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCPY010000217 DACCPY010000000
VERSION     DACCPY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.234"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>530
                     /locus_tag="IVO69_002786"
     CDS             <1..>530
                     /locus_tag="IVO69_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7401494.1"
                     /translation="PENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEH
                     ENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHI
                     QKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKE
                     FPNQLIREGRTMEDPD"
BASE COUNT          193 a          100 c          111 g          126 t
ORIGIN      
        1 cctgagaatc ggaaacaagc caaagggcaa ttggcacgta caacccatga tcagtatgga
       61 gaggtcttgc atacctatac cattaagaat gcgctagaag atggctctgt cttaggattt
      121 caagtagagc atgaaaacac cgtggagccg acttcactag agaataaaat ctatcggaaa
      181 ttaaaagaag tagaaacata tgcagagtat tcatcagaac agatcaatcg tatgattgat
      241 caaatggaac ctgtcaaaaa agaaagttat ttggacccag ctgtttttga agcagatgaa
      301 catatccaaa aagtcattca taaaatgttc cgtccagata atgcctatac aaaatttgat
      361 tttcgaaatg gcaggccaac gaaatctgca atcttaacca cgagttccat tgacatggcc
      421 aaaaagtatt atcgggccat caaagagatg acgaaagagc ctgattggtt aacaaaagag
      481 tttcccaatc agctcattcg tgaaggacga acgatggagg atcctgattt
//
LOCUS       DACCPY010000218          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCPY010000218 DACCPY010000000
VERSION     DACCPY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.235"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVO69_002787"
     CDS             complement(<1..132)
                     /locus_tag="IVO69_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7401495.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCPY010000219          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCPY010000219 DACCPY010000000
VERSION     DACCPY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.236"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IVO69_002788"
     CDS             <1..>468
                     /locus_tag="IVO69_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7401496.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACCPY010000220          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCPY010000220 DACCPY010000000
VERSION     DACCPY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.237"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            82..>467
                     /locus_tag="IVO69_002789"
     CDS             82..>467
                     /locus_tag="IVO69_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7401497.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVS"
BASE COUNT          163 a           80 c           91 g          133 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatc
//
LOCUS       DACCPY010000221          464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCPY010000221 DACCPY010000000
VERSION     DACCPY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.238"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(15..>464)
                     /locus_tag="IVO69_002790"
     CDS             complement(15..>464)
                     /locus_tag="IVO69_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401498.1"
                     /translation="AELLRLETEKYIMNVYLERSELVENFVSYTICRKHYYSESEYYS
                     IFESMELMEELNSKDATVDDYYLVFTKFLTCGTESIVLMGMDYELAVPEVFLREYVEE
                     VDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQRFERKKGQ"
BASE COUNT          123 a           97 c           75 g          169 t
ORIGIN      
        1 ttcggtataa atggttattg accttttttc ctttcaaatc gttgatatgg cattcgttta
       61 cggtcaagtt cattttggat ttcttctgta tcgtcgtaag tgtaggtatc taaaaaatca
      121 gttaaagtcg tgtaccagtc gtcgcctggg ttatctactt cttctacata ttccctcaaa
      181 aatacttcgg gtacagcgag ttcataatcc attcccatta acacgataga ttccgtccca
      241 cacgtcaaaa atttagtaaa tacaaggtaa taatcgtcta ccgtcgcatc tttgctgttt
      301 agctcttcca ttaattccat gctctcaaaa attgaataat attctgattc tgagtaatag
      361 tgtttcctgc aaatcgtgta gctgacaaag ttttcgacca gttcactcct ttctaggtaa
      421 acattcatga tatatttttc tgtttctagt cgtagaagct ccgc
//
LOCUS       DACCPY010000222          459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCPY010000222 DACCPY010000000
VERSION     DACCPY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.239"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            11..>459
                     /locus_tag="IVO69_002791"
     CDS             11..>459
                     /locus_tag="IVO69_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401499.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLK"
BASE COUNT          179 a           59 c           90 g          131 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaa
//
LOCUS       DACCPY010000223          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCPY010000223 DACCPY010000000
VERSION     DACCPY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.240"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            81..>423
                     /locus_tag="IVO69_002792"
     CDS             81..>423
                     /locus_tag="IVO69_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7401500.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSG"
BASE COUNT          166 a           77 c           76 g          104 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcg
//
LOCUS       DACCPY010000224          416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCPY010000224 DACCPY010000000
VERSION     DACCPY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.denovo.241"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>416
                     /locus_tag="IVO69_002793"
     CDS             <1..>416
                     /locus_tag="IVO69_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7401501.1"
                     /translation="IAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVD
                     RKDLDNQTTTEFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVII
                     TTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          138 a           71 c          102 g          105 t
ORIGIN      
        1 attgccgcaa ataaacatca atcagggtat gtgtggcatg cgactggttc aggaaagacg
       61 ttgactagtt ttgtatctac taagctttta gcacgaaaat cggggatcga tcgaacgatc
      121 atgttagtgg accgcaaaga tttagataat cagaccacca cagagtttac gaaatttgct
      181 tcggagttta atactggggt ttcttctggt aatgccaaag cgaatagctt gatcgtggga
      241 acaggaagtg ccaaagagtt aagtgaaact ctgttggcag atgccaatgc gaatgttgtg
      301 atcattacaa cccgtcaaaa actagatgcg gcgttgaaat atgcaaagaa acaggaagag
      361 aaaaaaggaa ccaatcgctt ccaaaaatta atggggcaac atatcgtgtt tgtagt
//
LOCUS       DACCPY010000225        23978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.041, whole genome shotgun sequence.
ACCESSION   DACCPY010000225 DACCPY010000000
VERSION     DACCPY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.041"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1009
                     /gene="rpsA"
                     /locus_tag="IVO69_002794"
     CDS             <1..1009
                     /gene="rpsA"
                     /locus_tag="IVO69_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7401502.1"
                     /translation="GDVLDLVVISTIGKDKENGSYLLSKRRLDAKKVWEDIERDFQEG
                     KVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYKGKTLSFKIIEIEPSEN
                     RLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFGAFVDLGGIDGLVHVSE
                     ISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQPGPWSEIDTKAPVGTVL
                     DGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLHEGDEVKVKVLEIHPEE
                     HRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILGDALKEEVEEKATDDSE
                     K"
     gene            1191..2501
                     /locus_tag="IVO69_002795"
     CDS             1191..2501
                     /locus_tag="IVO69_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7401503.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            2744..3019
                     /locus_tag="IVO69_002796"
     CDS             2744..3019
                     /locus_tag="IVO69_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7401504.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            3254..4300
                     /locus_tag="IVO69_002797"
     CDS             3254..4300
                     /locus_tag="IVO69_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401505.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            4302..5561
                     /locus_tag="IVO69_002798"
     CDS             4302..5561
                     /locus_tag="IVO69_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7401506.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            5567..6148
                     /locus_tag="IVO69_002799"
     CDS             5567..6148
                     /locus_tag="IVO69_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7401507.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6230..7105)
                     /locus_tag="IVO69_002800"
     CDS             complement(6230..7105)
                     /locus_tag="IVO69_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7401508.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            7253..7585
                     /locus_tag="IVO69_002801"
     CDS             7253..7585
                     /locus_tag="IVO69_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7401509.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            7697..8905
                     /locus_tag="IVO69_002802"
     CDS             7697..8905
                     /locus_tag="IVO69_002802"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7401510.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            9059..9514
                     /locus_tag="IVO69_002803"
     CDS             9059..9514
                     /locus_tag="IVO69_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401511.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            9876..10151
                     /locus_tag="IVO69_002804"
     CDS             9876..10151
                     /locus_tag="IVO69_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401512.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            10164..12242
                     /locus_tag="IVO69_002805"
     CDS             10164..12242
                     /locus_tag="IVO69_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7401513.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            12402..14288
                     /locus_tag="IVO69_002806"
     CDS             12402..14288
                     /locus_tag="IVO69_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7401514.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            14300..15247
                     /locus_tag="IVO69_002807"
     CDS             14300..15247
                     /locus_tag="IVO69_002807"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7401515.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            15267..15794
                     /locus_tag="IVO69_002808"
     CDS             15267..15794
                     /locus_tag="IVO69_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7401516.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(15857..16510)
                     /locus_tag="IVO69_002809"
     CDS             complement(15857..16510)
                     /locus_tag="IVO69_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7401517.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            16642..17484
                     /locus_tag="IVO69_002810"
     CDS             16642..17484
                     /locus_tag="IVO69_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7401518.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            17642..18538
                     /locus_tag="IVO69_002811"
     CDS             17642..18538
                     /locus_tag="IVO69_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7401519.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            18541..19254
                     /locus_tag="IVO69_002812"
     CDS             18541..19254
                     /locus_tag="IVO69_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7401520.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            19274..19792
                     /gene="msrA"
                     /locus_tag="IVO69_002813"
     CDS             19274..19792
                     /gene="msrA"
                     /locus_tag="IVO69_002813"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7401521.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            19789..20016
                     /locus_tag="IVO69_002814"
     CDS             19789..20016
                     /locus_tag="IVO69_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7401522.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            20168..20719
                     /gene="lepB"
                     /locus_tag="IVO69_002815"
     CDS             20168..20719
                     /gene="lepB"
                     /locus_tag="IVO69_002815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7401523.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(20802..21344)
                     /locus_tag="IVO69_002816"
     CDS             complement(20802..21344)
                     /locus_tag="IVO69_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7401524.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(21581..21979)
                     /locus_tag="IVO69_002817"
     CDS             complement(21581..21979)
                     /locus_tag="IVO69_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7401525.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            22239..23099
                     /gene="ylqF"
                     /locus_tag="IVO69_002818"
     CDS             22239..23099
                     /gene="ylqF"
                     /locus_tag="IVO69_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7401526.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23092..23859
                     /locus_tag="IVO69_002819"
     CDS             23092..23859
                     /locus_tag="IVO69_002819"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7401527.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPCYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            23912..>23978
                     /locus_tag="IVO69_002820"
     CDS             23912..>23978
                     /locus_tag="IVO69_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protecting protein DprA"
                     /protein_id="HAP7401528.1"
                     /translation="MMYQIEENLLKLAYTKGISSQG"
BASE COUNT         8289 a         4061 c         4959 g         6669 t
ORIGIN      
        1 cggtgatgta ttagatttag tcgttatcag tacgatcggc aaagataaag aaaacggcag
       61 ctatctactt tcaaaacgcc gtttagatgc taaaaaagtt tgggaagata tcgaaagaga
      121 tttccaagag ggcaaggtca tcgaagcacc tgttacaaat gttgtcaaag gcggtttagt
      181 cgttgacgtc ggtgtacgtg gttttgtccc tgcatcaatg gtggaagatc attttgttgc
      241 agacttttca gactataaag gcaaaacatt gagctttaaa atcatcgaaa tcgaaccatc
      301 agaaaatcgt ttgatcttat cgcataaagc ggtagtggaa aacgaaagag aagaaaagaa
      361 aaaagaaatt ctttcaacta tccatgaggg agatgttctc agtggacgtg ttgcacgtct
      421 tactgatttt ggcgcatttg tcgacctagg cggaattgac ggtctagtcc atgtctcaga
      481 gatttcacat agtcatgtag caaaaccaag tgatgtattg gctgtcaatg atgatgtaaa
      541 tgtcaaagtg ttgtctatca atcctgaaca agaacgtgtt tccttgtcaa tcaaagacac
      601 acagcctgga ccttggtcag aaatcgatac aaaagctcca gttggcacag tattagacgg
      661 aacagttaaa cgtttgacaa gcttcggtgc ctttgttgaa gtgttccctg gagtagaagg
      721 gctagtccat atctctcaaa tttcacataa acatattgcg acaccacatg aagtgcttca
      781 tgaaggtgac gaagtgaaag tgaaagtatt agagatccat ccagaagaac atcgtattgc
      841 attgagtatc aaagctttag aagaaaaacc agcttcagaa gaagaaccaa aaaatgttga
      901 gtcttatgaa ttaccagaag aaaatactgg atttactatg ggagacattt taggtgatgc
      961 gttgaaagaa gaagtagaag aaaaagcaac cgatgattct gaaaaataat tgattttgta
     1021 tctgtgactg agatgaaagc agctgctttt gtctcagttt ttcttttgaa aagcgattag
     1081 tatttcaata aggaataaaa atacttcgtt taattgacaa tctatgcttg aaagttggca
     1141 atgcattcgt taaactaagt gagattggaa acttgtgagg aggaaataat atggcaaatc
     1201 caacaattgc aatcgttggc cgcccgaacg tcggtaagtc tacgattttt aaccgcatcg
     1261 ctggtgagcg tatctcgatt gtcgaagata ctcctggagt aactcgtgat cgtatttatg
     1321 caaaaggtga atggttaggt cgagaattca gcgtgatcga cacaggcgga attgatcttg
     1381 gcgatgaacc ttttatggat cagatcaagc atcaagcaga aatcgcgatt gaagaagcag
     1441 atgtaatcat ttgtgtagtc agcggacgcg aaggcgtgac agacgcagat gagatggttg
     1501 cgaaaatttt atacagaagt aataaaccag tgattttagc agtcaataaa gtagataatc
     1561 cagaaatgag aaacgatatc tatgaatttt attccttagg gctaggcgat ccttatcctg
     1621 tttcaggaag ccatggacta gggattggtg atattttaga tgaagcagtg aagtatttct
     1681 ctgaagagac tgaagaagag gacgacgaca cgattaagtt tagtttgatt ggtcgcccaa
     1741 atgttggtaa atcctctttg atcaatgcga ttttaggaga agaacgtgta attgtttcgg
     1801 atattgaagg aacgacccga gacgcaatcg acacccactt cgtttctgaa agtggtcaga
     1861 aattcttaat gatagataca gctggtatgc gtaaacgcgg aaaagtatac gaaaatactg
     1921 aaaaatacag cgtgatgcgt gcaatgcgtg ccattgaccg ttctgatatc gttttgatgg
     1981 tactgaatgc tgaagaaggg attcgggaac aagacaagcg agtagcgggt tacgcccatg
     2041 aagctggtcg tggaattatt atcgtcgtga ataaatggga tcttgtaaag aaagaaacca
     2101 atacaatgcg tgatttcgaa caagagattc gtgatgaatt ccggtatctt gattacgctc
     2161 caatcgtttt tgtttctgca gtgactaaac aacggttgga acgtctgcct gaaatgatcg
     2221 aacaagtaag tatgaaccag aatcttcgta tctcttcggc tgtgttgaat gatatcatta
     2281 tggatgctgt agcaatcaat ccaacaccaa cagataaagg aaaacgcctg aagatttttt
     2341 atgctacaca agtcgctgtc aaaccaccta catttgtcgt ttttgtcaat gaagaggaat
     2401 tgatgcattt ttcttatgaa cgtttcttag aaaatcaaat cagaaaagca tttacttttg
     2461 aagggacgtc catccgaatt atccctcgta ggagaaaata ggcattttac catatcgacg
     2521 tttttttttc aaaagaaaaa gacagtttcg tttgttacgt ttctatgact aaaaaaagca
     2581 tgtgaaaatt ggtaaaaacc ttgctattaa gcggttcata tgatatcgtg ataacagaat
     2641 attgaagtta atcaatattt ctttttaaac ttctggacca ctcagaaagt tttaactaaa
     2701 tgatatttct atcatatctc ttgtggagga ggtgaatcta tccatggcaa acaaagcaga
     2761 attaatcgaa aaagttgctt cagctactga tttaactaaa aaagatgcaa ctgcagcagt
     2821 agatgctgta ttttcaacta ttcaagatgc tttagctaac ggcgaaaaag ttcaattaat
     2881 cggttttggt aactttgaag ttcgcgaacg cgcagctcgc aaaggtcgta acccacaaac
     2941 tggtgaagaa atcgaaattc cagctagcaa agtacctgca ttcaaaccag gtaaagcatt
     3001 aaaagacgct gttaaataag acagttgaaa aagcgcttgt ggctctaggg ctacaagtgc
     3061 ttttcttttt atttcctagc agaaaaggaa taaataagag acagagattc cgaatccgta
     3121 tgtcatacat agaaaacaca tgtattttat ttaattttta tttatttgca agttatgagg
     3181 tgagctttgc tcttatcttt tctagaattt gcttactaat ggtaaaataa aaataagaag
     3241 aggagggatg cgtatggaac ttgattatca agtagaagat gcgttgacga aagtgtttta
     3301 cggagaaacg ctagtcagtc agctaagaga actttccatt gatcagccaa tcttattttt
     3361 aacgaatcag cgctattatg atttgtttgc agataagatc aacccgttat ttgcaaatca
     3421 agcaaacaat gactggtata tttgtgccaa cactcaatgc aatcatttaa cagaattaaa
     3481 gaacctgctt gatttcgtca aacgctatcc cgaaaatcaa tccatgttta tcatcggatt
     3541 tggaaatgaa gggatcatgg atctggcagg tttttttcaa aaacatacaa cccttgattc
     3601 atcactatgg atgatccctg tatcgatccg ttctctaagc cgagcattaa caccgcagcg
     3661 gacgattttg cagctaccaa atcgtgtagt gctgcaaacc atcaatctgc cagaacgcat
     3721 cgtttatgat caaacaatca gcgagcggca ggtggacgga aagcagattg actttttgac
     3781 ttttatctgt tgcggaatga tttgtgatca tcgttttttg caaaatctgt ataaaaacta
     3841 tccaaataaa aagcaattaa cgactcggcc gtttgccggg atgatcgaag aaatgatcta
     3901 tttttatcaa ttagaggcag aaaacctgtc aaattatggt aaactgtttg aacaagcttt
     3961 ttatctgacc gaaaatggcc atctactttc tgctagtatg aaaaaatttt taggaatgat
     4021 gtttcatttc gtgtggaatc tccaagcata tccttttgat ttccaactaa aaaacttttt
     4081 tgtgtggctg gacttgcttg gctatccaat ccactggctg gaacagattt caaagatgga
     4141 gtatttggaa aaagttcttt ttttggcaga aaaaagcaaa aaaatacttg ttttagaaaa
     4201 aataggtata atagacggat accaacatcc aaacgaaaaa gaattattga gaacaatgac
     4261 ggtatttcaa caaatcgtca gtgagataag agggaattga aatgaataca aatagcgaaa
     4321 aaatgttgca agctttatca gatgaagact tgtcacaagc acaaatcttt ttagaaaaag
     4381 cattaaaaga agacccagca gatatattag ctgaactagg cgaagaattg ctggctattg
     4441 gctttttgat cgaagcaaaa cagatttttg aacaactgat caaccaatac ccagaaaatg
     4501 acggactgaa catcccgtta gcagaaattg caatcgaaga taatgagatc gacttagctt
     4561 ttgaatacct ggaaaaagtg gataagcaaa gtgattacta tccgcaaagc ttattggcga
     4621 tagctgattt gtaccaagtt atcggaatcc ctgaagtgag tgaagcaaaa ttgaaagaag
     4681 cagctgggtt gcttcctgat gaaccattga tccagtttgc gctagctgaa ttgtattttt
     4741 cagttgatcg tttcaaagag gcagcagtgg tttatgaaac attgttggca agcgggataa
     4801 atgaaatttc aggaatctcg atgcaggaac gtttaggaca atccttaagt atgcaaggag
     4861 aattcgaagc agctatcgta cctttagaag aagcactgaa agaagaaagg acagatgatc
     4921 gactgttcca gctagctttt acgaatcttc aattgaaaga aaatcagcag gcaatcaact
     4981 atctggaaga acttcgagaa gttaatccgc attatcaatc gttatatcta tatctaggac
     5041 aagctcttca agaagaagaa atgattgaag aagcacaaac agtgttagaa gaaggaatca
     5101 aagaaaatcc ttaccaagtc gaactttatc acctggcctc ggaaaatgct ttccgtctgc
     5161 atgatagaca gcgagcagaa gaacttttac tgaaagcttt agaagtagga gacaaacaag
     5221 atgaaacatt acttacttta agtaatcttt acttggatga tgaaagatat gaagatgtga
     5281 tacaagtgat caatcagatg gaagaaactg ctaatcctta tgcagaatgg aacctagccc
     5341 atgcttataa tgaattagaa gactttaccg ttgcagctgt acactatgaa caggcttatc
     5401 atgaactatc acatgaacca gatttcttga aagagtatgc ctttttctta agagaagaag
     5461 ggcagctgaa aagagcgcaa gaattattga cttattattt aacacttgaa ccaggagata
     5521 tggaggcact gtcattatta gatgacctaa cagagaggtg agaaaaatgc taattgatgt
     5581 acgggaaaag aagaattttt taacgtggat ggtcaatcat gtatctttta gccgaagaga
     5641 agttttttgg atcttgaatt atcttgccaa tcatgaagcg attttaagca atgtccactt
     5701 tgttgaaggg gcgaattttg cggatcgagg actgcagatc agtgatctat ctgttgaagg
     5761 ggaacccatg aaattgtttt tgcagggaaa agaatttacg gacacagacc agatttttca
     5821 tgaaatccgt ttgaattgga aaaagccttt atatgtggaa tgtatattcg aaaatgcttg
     5881 gcagacaaga gaatatctat ctatcttaga agacaatcca ttttcttctt gggattcttc
     5941 agtcagtgaa gaagatactg aattaattga acgatatttt caagaaaaag aagaagaggc
     6001 aaaactaaaa atgctttatg ctcaaatcga tcaagcacta gaagatggag atagggatgc
     6061 ttttttgaaa ttatctgatg aagttaaccg aaagatcctt ttgcagacac aaaagaaaag
     6121 taaccgggta agtgaacata acaattagtt aaaaatagta tagtactaaa aaagacaaag
     6181 gtcagctgga tgtggaatct ttcctcatag cctatctttg tcttttttct tatagttttt
     6241 tcagtaatct tgtctgtggc ttcaaataag taaagccttg cccttccact tcatgcacat
     6301 tgatgattga aaggaaagct ttttcgtcaa tctcgtggac gatcctctta gcttcagtta
     6361 attcactaga gctaacgacc atatagatga ttttttttga attaccagag aatgcgcctt
     6421 ctccagaaag gaaagtcaca cctctttgca gaccggtcat cagtagtggt gcaatctctt
     6481 cgcttttgtt ggagacgaca agtagacctt ttgcagaata tccgccatct aagatcgaat
     6541 caatcacacg actgaaaaca tacgaaacaa tcaacgtata catcatacgt ttcaagtcga
     6601 tataggttaa tgaagcaagt aagacgatga tatcaaatgc caatagggaa cgccccatag
     6661 tgatgccgta attttgttct aaaatccgag cgataatatc actgccgcct gttgttccac
     6721 ccatgcggta gacgatcccg cttcctacac ctgcaactaa acccgcaagt agagaaacga
     6781 tcagtagatc atgctgtaaa ttgatttcta gtggaaccct ttgccagatc cataaaaaga
     6841 atgacaaaga aagtgtacct agaacagtat aggccaagga acgtttaccg aggatctttc
     6901 ctcctagtaa aatcaatggg atgttgatta ataaagtcgt ataagctgga tcaataccaa
     6961 ataaagctct taagatcaag gtgatccctg agactccccc ttctgctaaa tgattagcga
     7021 tgttcaagta aacaagacca aatgcataaa tacttgttcc tataatgata aaaagaactt
     7081 cttttactgt ttttcgtttg accatatgaa ttccagcttt caattatgta ttttcacaga
     7141 atttccattt agaaattcct tcctaaatgt agcatggaaa ttcgattttc tcaattgctc
     7201 ttatctagga tttgcgttaa agttttggta agatagtttt ggagggaaaa agatggataa
     7261 aaagcgttct ttatacagta tgcagcagga agtggatgaa tatatccagc aattcaaggt
     7321 ggggtatttc tctccattag ctcagatggc tcgattaact gaagaagtag gtgaattggc
     7381 aagagaagtc aaccacactt atggagaaaa aagcaaaaag gcttctgaac cagttaactc
     7441 ggtagcagaa gaacttggtg atgttttatt cgttacgatg atcatggcta attcattgaa
     7501 tatcgactta actgaagttt ttgaaaaaaa catggaaaaa ttcaatcaaa gagaccataa
     7561 tcgctttgag cgtaaggacg agtaaacaag atgaagtggt cctggccttg aagactctga
     7621 aacggacttg tcaaacagtc accatgcaac tgagtcttta tctcaggaca agaaacacgc
     7681 aggaaaggtt ggaaaaatga aattaaaaca attacctctg gaataccaaa aggcaattcc
     7741 agttctgaaa aaattagaga atgcaggata tgaagcttat tttgtaggtg gaagtgttcg
     7801 tgatatcttg ctgaaccagc caatccatga tgtggatatc gcaacaagcg catttccaga
     7861 agaaatcaaa cagctttttc caaaaacgat agatattggg atcgaacatg gaactgtgtt
     7921 ggtattagaa aatgatgaac aatatgagat caccacattt cggacggaat caacttatca
     7981 ggactaccgt agaccagatc atgtcgaatt cgttcgttcg ttggcagaag atttgaaaag
     8041 aagagatttt acgatcaatg cgtttgcttt gaaagaagat ggagaaatca tcgatttgtt
     8101 cgatggtctg caagatctag aagaacagat cttgcgagct gtaggcaatc ctcatgagcg
     8161 gttccatgaa gatgccttac gaatgatgcg cgggttgcgt tttgtcagtc aactagggtt
     8221 caaattagag gaagaaacct tctacgccat caaagaaaac cacgagctat taggtaaaat
     8281 atctgtagaa cgtataaatg tggaattcat caagatgctc ttgggaaatg aacgggccgc
     8341 gggaatccat gcatttattg aaacagagtg ctacatttgc tgtcccggtt tgaaagaaga
     8401 aggcgaagca cttttacgtt ttgctgaatt accatccgtc ccattgaaag aagagagcca
     8461 agcatgggca ttattgatca gccaactgga tctaaaagaa aatgagattc gttccttttt
     8521 gaaatcgtgg aagtgttcta accaaatgat taaagacgtt caagcacttg tccaaggact
     8581 acgacaacga gaaactggag ctttggaacc attcgaattg tacttcttag gcaaaaaaca
     8641 tgcactgcaa gtagaagaat tgatgatcta ttttgacgaa acacctcaga tggatgccgt
     8701 aggagaatcc tatgatcgtt tacctatcaa gtcattaagc gaattagcag tagatggtaa
     8761 aatcttgcta gaagaaactg gaaaaaaacc tggtaagtgg gttagtgaag ctttgcaatt
     8821 agctgaaaaa gccgtcgtag aacaaagaat aaaaaatgat aaaaaacagg ttttggattt
     8881 tttatcgaaa gaaaaattga tttaaaaaac actagactgt atctgctcag ctttgttggc
     8941 tacttaaagt gggcagatac gtcattagaa gaaaaaacat aagatgtgac cacttacata
     9001 aatttgtgct aaactgaggt agtacaagaa taaattacat cataaaggag tctttttaat
     9061 gagtaaagaa atgacagcgc taaaatttta ttttagaaat ggagaaacct ggacgatcaa
     9121 ccgccgtcat atcggcgatc tttggatcaa acaaatcaca acaagttttg gacgaatcaa
     9181 tggcagtgaa tttgttgaaa tccatccgtg tgctggattt aaaatcgaaa tcttccatga
     9241 aggggacgca gttgcgacac atgatatcaa cctaggcgga ctggaaatgg gcatgtttaa
     9301 tcgcgcattg aaatacgaag atatcgagcg aatggaaatc ctttaccgaa atggcacacc
     9361 tgatcttgtg tacttcccat atctagataa aggaacagaa ggattagaca atcaatatca
     9421 atcaacaaaa atcagcgaaa aaacaggtaa cttgtatatc gtgatcaatc cagatcaacg
     9481 tgtagaagat gtatacgggg aatttttcga ataaatcgat ttttacgcaa aaatgaggct
     9541 gtgaaagaga tgtgtcttat tactactgat aggctgtctc ttccacaccg tttattcaga
     9601 ataagggact gtgacaagat ttttgtcgca gccccatttt tttgttgtga taaaagaaca
     9661 agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga aacttttatc tttcttaagt
     9721 tttacttgat atctgatact atttggaata gaatgaggcg ttgaaaaatt tatggaaatt
     9781 tctaactagc aataaacgat gaaacaatga aatatttttc atccaaaaag aactttgcta
     9841 taatgatgga agtttgtgta taggagatct ggaaaatgag agagagtttt gaacaacaaa
     9901 aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga tgaccgccgt caaatacttt
     9961 gtaagttgcg taaaagaaat atattgatgt atcgtcagtt ggaacgtctg aaacatgatc
    10021 tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga ggggaaccaa acacaggttg
    10081 aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa
    10141 ataaaaaata aaggggatgg acgatggaat ttgctgagtt agttattgta tttgcagtaa
    10201 cgatcacctt atcgaacata gctagccgca ttttgccaat gattccagct ccactgatcc
    10261 aaattttcct tggagtggta ttaggtctga cagagtgggg acaatcaatc gattttgaac
    10321 ctgaattgtt cttagttatg attattgcac cattgttatt tagagaagga gaaaaagcag
    10381 atatttcttc gattctcaaa aatttcggga caattttgtt cttggcattt ggaggagtca
    10441 ttttgaccct tgtcggggta ggtgcgacgt tatcattcct gttgccaagt gtcccacttg
    10501 ctgcctgctt tgcttttgga gcagcgttag gtccgacaga tgcggtagca gtcagttctc
    10561 tttctggacg agtgaatatc cctaaaaagg cgatgcatat tttggaagga gaaggtcttt
    10621 tgaatgatgc atctggtgtt actgccttcc agtttgctct tggtgcgctg atcaccggat
    10681 ctttttcagc agtaaacgcg gggatgagtc tagtagtttc tagtattggg ggagctttga
    10741 tcggcttttt gcttgtctgg ttcaaacaaa aaataattca tttgatcgaa aaagcatcag
    10801 cacaagatgt cacagggtat ctgctgatcg aacttttgct tccttttcta gcctatgtat
    10861 tggcagaggc atttgatgta tctggaatca ttgctgcagt ggcagcaggt attctccaag
    10921 cttctggttt taggaaaatc tctgtgtttg atgcagaatt gtcgagcttg tcccacagta
    10981 catggacaac tattgctttt acactgaatg cattagtatt tatattttta ggaatcgaat
    11041 tgacgcaagt gttctcgcca gtatggggag acggtttata tccaaacggt cttttattag
    11101 cgattatcgt attaatctct gtcatgcttt ttgtgatcag attcatttct atcagtcttt
    11161 tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct
    11221 taacatttgg cggtgtaaag ggaacagtca gtttggctac gatttttatt ttgccatttt
    11281 caatcaataa tatgatgttt taccagcggt ctttgctttt gtttctgacg gcgggtgtca
    11341 tcttagtgac tttggtcata gggatcattg tcttgcctat gctgacggaa acagaggaag
    11401 cgaaaagcac ggatttgaat gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa
    11461 aagaaataaa ggaaattgat cgtaatacaa aagaattttt agccactgaa gcagtgatag
    11521 aaaattatca agaacggata cgtgatctgt acttggaaga tttaaccgat gatgaaaagc
    11581 aagaagtaca agaaatacaa gcactgattt tgtcgatcga acgggatgga ttggatgaaa
    11641 gttatcgttc gggcaagctt tcttctaatg gttatcgttt ttactcccgt tttctttctc
    11701 gattcgaaca ttctatcaca agccagattt tgtcttttat cggcttttgg ttcattgttg
    11761 tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt ttggcaaaga agatatgctg
    11821 atcgtcagac atttattagt gatgaagata tccaagaaat cagagagaca tatcaaaaaa
    11881 atactcagtt gattattgaa agtttagata acttgactga tatttatgat gaaacgatgg
    11941 ttcaattttt tatccagcaa cgcaaacttg aaggaataaa aatgaactcg gggaatttga
    12001 tttcttcgtg gatgatccag caagatagtc tgtttacgaa aaaaatgcta agaggttatt
    12061 atcttgaaag aaaaatcatt gatgaatacg aagttgctga aaaaatcacg acgttttctg
    12121 cgaacagcta tcgcaggaat atcaatttgt tagaatctta tacgatgaac aaaccatctg
    12181 ataacttttc tttccgattt gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat
    12241 aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca tatcagaaca ggatcgaatc
    12301 ggtcctgttc tttttttgag aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt
    12361 tcactatttt tatgatacaa tatccaagat aaggagggaa catgttgaaa gaattaaaag
    12421 taactgacct caaaaaaact tatggtgaaa aagatttgtt tgaccagatt tcttttttgg
    12481 tccacgaaaa agaccgtatc gggttgattg ggacgaacgg gacgggtaaa acaagtctct
    12541 taaatattat tgccggtatc gatagcagcg acggtgatcg gcaaacagtg ttttatccaa
    12601 ctgattaccg tattggttat ctttctcaag cagctgaatt ttccgatgaa ttaactgttc
    12661 tccaagcagt ctttcaagga aacagcccct tgatccaaac tgttcgagca tatgaggaag
    12721 cattgatcga actaggggaa aacggagaag accctgatgt gcaaaaacgt tatgctaaag
    12781 cagaagaaca aatgaataaa gaagatgctt ggactacaga taccaacgca aagattattt
    12841 tgcaaaaatt aggaatcagt gaattagata aaaaaatcag tactttatca ggtggtcaga
    12901 aaaaacgagt tagtctggcg caagtgttga ttgatgaacc cgatttacta ttactagacg
    12961 aaccaacaaa ccatttagat tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat
    13021 accgtggcgc actattgatg gttacccacg atcgttattt tctagataga gtagcaaacc
    13081 gaatcttcga actttcattt ggaaaattat acgagtataa aggaaattat gaagcttacg
    13141 tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc
    13201 tttataaaca ggaattagct tggatgaggg ctggcgtaca ggcacgtggc accaagcagc
    13261 aagcacggat caaccgtttc gaagatctaa aggagaactt gtaccaagta aaccaagaag
    13321 atgatttaga gctgaaccta gcgacacaac gtttagggaa aaaagtactg gaaatcaaag
    13381 atggcagtta ccgtataaat gaccaaacac tgctagaaca tttagattta ttgattcaat
    13441 ctcgggaaag actaggtatt actggtaaaa atggtgcggg aaaatcgaca ttgcttaata
    13501 ttctagctgg cagaattccc ctggatagcg gtacaatgtc tatcggtgaa acggttcatc
    13561 ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga taaacggatg attgcttact
    13621 tacaagaagc tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga gtagcagaat
    13681 tacttgaacg atttttattt ccaagattta tgcatgggac actgataggc aaactttcag
    13741 gcggagaaaa acgccggtta tttttgttga aactgctgat tcagcaacct aatgttttat
    13801 tattggatga accgaccaat gatttggaca tagcaacatt gacgattttg gaagattatt
    13861 ttagaagttt tccaggtgct gtgatcactg tttcccatga ccgttatttc ttggacaagg
    13921 tagcagataa gttattagta ttccaaggaa acggaaaaca agaactttat tacggaaaca
    13981 tgtccagtta tttactgaag caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa
    14041 aaatacagag caaggaacct gctgaaaaga agaaattatc ttacatggaa caaaaagaat
    14101 gggaaacgat tgaagatgag attgcagaac ttgaagaaaa aatctcattg ctccaagaag
    14161 agatgaatca tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg
    14221 aaacagaagc acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag
    14281 aagattagga ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga
    14341 agaagggcat cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca
    14401 aatgcggttt gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg
    14461 attgatcaaa agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt
    14521 acaaaataaa aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga
    14581 ctataatgga ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa
    14641 tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt
    14701 tgctgaaaca tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac
    14761 aaaagacggt ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc
    14821 tgattctaga agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt
    14881 gcctccttgt catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt
    14941 gtatcaacgc agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt
    15001 gctgactcac ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt
    15061 aggagatgct catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga
    15121 aatacgcgca tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga
    15181 gatggaagat atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc
    15241 tgtttagaat cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg
    15301 gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca
    15361 aacaaatgac agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta
    15421 gccgtccgtt gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag
    15481 aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg
    15541 gtatcttttt catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga
    15601 tattataccg tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg
    15661 actggtctga ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt
    15721 atccgcatca atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg
    15781 aattgacgat ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat
    15841 agctttattt tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta
    15901 acataacaaa caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga
    15961 atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa
    16021 gagagtgata aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga
    16081 tcgtagatac ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc
    16141 caagaatcgc aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac
    16201 tcagcaaaca aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga
    16261 atacttggaa cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta
    16321 aaaataaaag aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat
    16381 gcaagtgggc accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa
    16441 ttccatgggt gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg
    16501 ttttttccat gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct
    16561 gttatactaa aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta
    16621 aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg
    16681 atggaaaaaa atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc
    16741 gacggaacag tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg
    16801 gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa ttggagaatt catctcgatg
    16861 tatgatgagc taggagaaga cggcagtgaa gtgatttcga tccatatgac aaaaggcttg
    16921 agtggaacag tagaagcagc aagacaagcc agtaatctaa caaaaacgaa agtaacggtt
    16981 atcgacagtg ataccactga ccaaggatta tcttttcagg taattcgagc agctgaatta
    17041 gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag tcaaaaaagt acaagacaat
    17101 acaaaattat acatcggaat atccacacta gataacttgg taaaaggtgg aagaatcagc
    17161 cgaacaactg gtttgctgtc tagtgttttg aacatacgcg tggtgatgaa tttcgcccat
    17221 tctgaattaa tccctgtaac taaggggcga ggaaagaaaa cgttcgataa atggtttaat
    17281 aatttgaaaa atgaactgaa agaattgcca aatgtgcgac aaatcggtat ttcacatgcg
    17341 gatgcaaaag aattggcgca agaattcaaa gagggtttgc aagaattatt cccggacatg
    17401 catatccctg tactgcatac gaatccggtt atcgctacgc atactggtaa agacgcgttt
    17461 gcaatcatgt attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg
    17521 cagcttattc tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag
    17581 actacctttt tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa
    17641 aatgaaatta acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat
    17701 catctatgca ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac
    17761 tactgtgaga gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc
    17821 gctgactgaa ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa
    17881 tgatctaaaa gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg
    17941 tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat
    18001 caaaacagct gattttatca cactgactgt tggcggtaat gatttgatga aagttatcca
    18061 aaataattta ttcgggttaa cggtcaattc ctttaaaaag ccactgaaaa actatcagga
    18121 aaatgtcaca gaactattgg aagaatttcg atccctcaat aaaaccgcac ctatttacat
    18181 tttaggcatc tacaatccat tttatttgaa ctttcctgag atcactgaca tgcagacaat
    18241 tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa aaaatgccga actgctactt
    18301 tatcccaatc aatgatttgc tttatcaagg attgaatcag gaagtaggtg tcacagaaga
    18361 aggaacagat caaacaacta gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct
    18421 ctatgaagaa gatcatttcc atccaaataa tttaggttat caattgatgg caaatgcagt
    18481 gagagatcag ctgatcgaga cacaaaacca atggttagag aaggaggatg atcaataaac
    18541 atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga aaacaaatta ctggaaattc
    18601 gccttcctta tccttcttgg ggtagtactt ggttcgaccg tatttttagg cagccgtatt
    18661 tttgctaatc gggaaccaga actcccagaa ataccagctc ttacggaaag acaaggagat
    18721 cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc aaatcatcag ctttttcctt
    18781 agtgagtatc aaaaagatag tgatattgaa tataaatttt atttggaaaa tgaagcacta
    18841 ctcaatggta ctttcgaagt actaggtttt cctatcgatt tctatttata ttttgatccg
    18901 tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa gtctttctat cggaacattg
    18961 agtttgccaa tcaaagatgt gatgaatatg atcaagagaa actataaatt accagattgg
    19021 atcgaagtca atacagatga tctgactatc atgcttaggt tggatcaatt ccgaatgcaa
    19081 aacgggatgt atattaaagc agataaaatc gacttagtaa atgatgacat tcggtttagt
    19141 ctatatttgc caaaagatac ctctgatgct tcaaacaact caaataccaa caaaactagc
    19201 accagcagta ctgaaagcaa gacaaaagaa aataaaaaat catcggatga gtagtattag
    19261 agggggaatc ataatggaac gagcaatatt tgcaggaggc tgcttctggt gtatgatcca
    19321 gcctttcgac acgcttcctg gtatccatac agtgatgtca ggatatactg gagggcatgt
    19381 accagatccg acttatgaac aagtaaaaag ccaaacgact ggacatacag aagcagtgga
    19441 gattatctat gatccaacat tgatttcata tgaacaatta gtagaacttt actggcagca
    19501 aactgatcca actgacgctt tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt
    19561 gatcttttat ttgacagaag aacagcggat cattgctgaa aaaagtaaga aacagttagc
    19621 tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc gaaccagccc aaccatttta
    19681 tctagcagaa gaagaccatc aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa
    19741 ccatgcacgc cgggctgcct ttatcgaaga aaattggagg aacgaagggt gaatcgaagc
    19801 ttttttcatt atttaatgac actgcgaggc ggtggcacac atgatgcttt atctgttttc
    19861 gctacagaag ccggtaaaga catccaattt cctaagcaca gctctagcta cgaagaaatt
    19921 tctaattatc tggaactgaa tgtcgactat ttgccgtcca tggatatctt tgacgaagca
    19981 tgggaaaaat atttagaaaa taatcatgac atataagaga tagttttgta ctttatgatc
    20041 aaaaaagttt tttgtccaat agcctaattg aaagaattat ttttatttca gctatctagt
    20101 cgcatttttt tgatattttt cttataataa agaaagaatg aaaaaaagag ggatggattg
    20161 atagagattg acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt
    20221 acttgtttct gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt
    20281 ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt
    20341 ttcggagatc agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat
    20401 caaacgagta atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat
    20461 caatgggaaa gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa
    20521 tgcatcctat acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga
    20581 agataattat tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg
    20641 cacgatcaat aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga
    20701 aataaaatgg atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta
    20761 ttgtttaatc caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac
    20821 acttttctag cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt
    20881 tggatcatat tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa
    20941 acagacatca gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca
    21001 gcgatgacgg aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca
    21061 ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa
    21121 tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc
    21181 ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc
    21241 ttgtataaga tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc
    21301 atttcgggca atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg
    21361 tcagttaata ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt
    21421 acatacactg gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa
    21481 aaaagagatt gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct
    21541 ttgttttttt gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg
    21601 gttctaatgt atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca
    21661 ttcccatatc tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt
    21721 ttacttcatc cgcattttca acactaaacg aaatcaaagc accacttgtt tttttattat
    21781 cagctatttc tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc
    21841 aaaaagtgtc atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt
    21901 taaaacccag cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa
    21961 caaaaaccat actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt
    22021 atttaggaaa cgctgtcaaa aatttagatt catatataga aaccaaaata aaaatttgca
    22081 tcatcaaaaa agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag
    22141 atggaatttc tttataataa aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag
    22201 ccggggaata ttccggtatg aaaggaatgg agagaacaat gaccatccaa tggtttccgg
    22261 gacatatggc aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat
    22321 ttgaattggt agacgcacga ttacctttat cttctcgtaa tccaatgcta gatcagatca
    22381 ttcagcaaaa acctcgcctt gttttattga ataaagcgga tctggcagat ccacaacaga
    22441 caaagctgtg gcaagaatat tttcgtaaac aaggccatct tgcattagct atcacggcac
    22501 aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa agaagcattg aaagaaaaac
    22561 gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta
    22621 ttccaaatgt tgggaaatct acgttgatga atcgactagt aggcaagaaa attgcacaga
    22681 caggaaataa gcctggcgta acaaaaggtc agcagtggct tcgtttaggt aatgagttgg
    22741 aattgttaga cacaccaggg attttatggc ccaaatttga agatgaagaa atcggcaaaa
    22801 aactagcgct gacaggcgcg atcaaagacc agcttgtcca tttagatgat ttagcgattt
    22861 acggattaga cttttttgcc cgatattatt caggaagaat cagcgatcgt tatggtttat
    22921 caatcgagca agaagaaaaa ttggcaccgg aattactgat ggatattaca gcaaaacgtg
    22981 ggtataaaga agactatgat cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa
    23041 aacttggacg atacacactg gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa
    23101 tccatccaaa gtatcaaaga acgattgaaa agcatacaga gaatagatga tcctacacta
    23161 gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc ttttgaacga
    23221 cgattgaaaa aggaagcatt aattaaggaa aaatatgaag aaatgttttg ctttgaaaag
    23281 caagggtacg cacaaggtca ccaatggatt gccggcattg atgaagtagg aagaggacca
    23341 ttagctggac ctgtcgtggc agctgcagtt atcttgccga aaaatgagat catattaggg
    23401 ctgaatgact ccaaacaatt atcagagaaa aagcgagaac ttctttatca acaagttcaa
    23461 gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc agacaatcga tgatgtgaat
    23521 atttatgaag caagtaaatt agcgatgatc caagcagtag aaaaattgtc cattgtacca
    23581 tgctatttgt tgatcgatgc gatgaccttg gatttgccta ttcagcagga aaaaataatc
    23641 aaaggggatg cacgatctgt gtcgattgct gctgcaagta tcattgctaa agtatatcga
    23701 gaccaattga tgaaagaata tgacgtgctt tatcctggat atggtttctc gcataatgca
    23761 ggttatggaa caaaggaaca cttagaagga cttgaacgac aaggaattac gccaatccat
    23821 cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc ttttacgtat
    23881 cttattagaa agagagacga aaggcggttt tatgatgtat caaatagaag aaaatttatt
    23941 gaaacttgct tatacaaaag gaatcagtag tcaaggaa
//
LOCUS       DACCPY010000226        16588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.066, whole genome shotgun sequence.
ACCESSION   DACCPY010000226 DACCPY010000000
VERSION     DACCPY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.066"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            127..1167
                     /locus_tag="IVO69_002821"
     CDS             127..1167
                     /locus_tag="IVO69_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7401529.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1265..1453
                     /locus_tag="IVO69_002822"
     CDS             1265..1453
                     /locus_tag="IVO69_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7401530.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1511..1864)
                     /locus_tag="IVO69_002823"
     CDS             complement(1511..1864)
                     /locus_tag="IVO69_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401531.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2395..2817
                     /locus_tag="IVO69_002824"
     CDS             2395..2817
                     /locus_tag="IVO69_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7401532.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2814..3044
                     /locus_tag="IVO69_002825"
     CDS             2814..3044
                     /locus_tag="IVO69_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401533.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3542..3742
                     /locus_tag="IVO69_002826"
     CDS             3542..3742
                     /locus_tag="IVO69_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7401534.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3769..4962
                     /locus_tag="IVO69_002827"
     CDS             3769..4962
                     /locus_tag="IVO69_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7401535.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5030..6595)
                     /gene="guaA"
                     /locus_tag="IVO69_002828"
     CDS             complement(5030..6595)
                     /gene="guaA"
                     /locus_tag="IVO69_002828"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7401536.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6654..7577)
                     /locus_tag="IVO69_002829"
     CDS             complement(6654..7577)
                     /locus_tag="IVO69_002829"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7401537.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7773..8378
                     /locus_tag="IVO69_002830"
     CDS             7773..8378
                     /locus_tag="IVO69_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7401538.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8686..9702
                     /locus_tag="IVO69_002831"
     CDS             8686..9702
                     /locus_tag="IVO69_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7401539.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9843..11144
                     /locus_tag="IVO69_002832"
     CDS             9843..11144
                     /locus_tag="IVO69_002832"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7401540.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11190..11852
                     /gene="deoC"
                     /locus_tag="IVO69_002833"
     CDS             11190..11852
                     /gene="deoC"
                     /locus_tag="IVO69_002833"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7401541.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11873..12271
                     /locus_tag="IVO69_002834"
     CDS             11873..12271
                     /locus_tag="IVO69_002834"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7401542.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12337..13419
                     /locus_tag="IVO69_002835"
     CDS             12337..13419
                     /locus_tag="IVO69_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7401543.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13631..15193
                     /locus_tag="IVO69_002836"
     CDS             13631..15193
                     /locus_tag="IVO69_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7401544.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15186..16319
                     /locus_tag="IVO69_002837"
     CDS             15186..16319
                     /locus_tag="IVO69_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7401545.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16319..>16588
                     /locus_tag="IVO69_002838"
     CDS             16319..>16588
                     /locus_tag="IVO69_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7401546.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVAT"
BASE COUNT         5372 a         2982 c         3495 g         4739 t
ORIGIN      
        1 tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat
       61 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
      121 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      181 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      241 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      301 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      361 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      421 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      481 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      541 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      601 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      661 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      721 tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt
      781 actccagcag attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct
      841 ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt
      901 tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa
      961 aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat
     1021 gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag
     1081 gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta
     1141 agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct
     1201 tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat
     1261 tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg
     1321 aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga
     1381 aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac
     1441 gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga
     1501 attttactga ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca
     1561 ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag
     1621 tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg
     1681 atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta
     1741 aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca
     1801 ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac
     1861 gcatactaaa tcacctctat acattttact gttcctatat aaccataaac aggtatagaa
     1921 aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga
     1981 atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta
     2041 gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag
     2101 aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct
     2161 gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa
     2221 gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt
     2281 ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt tagtatgaag
     2341 ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa
     2401 ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt
     2461 gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg
     2521 ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact
     2581 gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt
     2641 gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg
     2701 gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt
     2761 catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa
     2821 cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag
     2881 caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca
     2941 tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg
     3001 aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg
     3061 aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca
     3121 tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat
     3181 aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta
     3241 caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc
     3301 atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg
     3361 tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt
     3421 gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat
     3481 ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat
     3541 aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc
     3601 agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc
     3661 tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc
     3721 aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac
     3781 ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct
     3841 aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa
     3901 gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg
     3961 acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc
     4021 agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg
     4081 gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag
     4141 tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca
     4201 attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa
     4261 aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac
     4321 aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga
     4381 atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta
     4441 ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca
     4501 aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc
     4561 gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt
     4621 attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt
     4681 atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg
     4741 ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta
     4801 ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga
     4861 gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact
     4921 gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata
     4981 agaagagcca gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact
     5041 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
     5101 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
     5161 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
     5221 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
     5281 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
     5341 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
     5401 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
     5461 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
     5521 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
     5581 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
     5641 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
     5701 cagatacacc tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca
     5761 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
     5821 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
     5881 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
     5941 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
     6001 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
     6061 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
     6121 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
     6181 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
     6241 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
     6301 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
     6361 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
     6421 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
     6481 attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac
     6541 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
     6601 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
     6661 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
     6721 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
     6781 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
     6841 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
     6901 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
     6961 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
     7021 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
     7081 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
     7141 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
     7201 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
     7261 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
     7321 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
     7381 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
     7441 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
     7501 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
     7561 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
     7621 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
     7681 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
     7741 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
     7801 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
     7861 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
     7921 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
     7981 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
     8041 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
     8101 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
     8161 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
     8221 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
     8281 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
     8341 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
     8401 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
     8461 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
     8521 gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag
     8581 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
     8641 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
     8701 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
     8761 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
     8821 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
     8881 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
     8941 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
     9001 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
     9061 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
     9121 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
     9181 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
     9241 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
     9301 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
     9361 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
     9421 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
     9481 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
     9541 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
     9601 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
     9661 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
     9721 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
     9781 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
     9841 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
     9901 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
     9961 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    10021 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    10081 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    10141 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    10201 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    10261 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    10321 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    10381 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    10441 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    10501 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    10561 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    10621 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    10681 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    10741 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    10801 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg
    10861 ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga
    10921 tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac
    10981 tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta
    11041 acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat
    11101 cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact
    11161 ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta
    11221 ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat
    11281 accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac
    11341 aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg
    11401 agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg
    11461 tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag
    11521 cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat
    11581 tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg
    11641 tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc
    11701 gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag
    11761 aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa
    11821 tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga
    11881 actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta
    11941 ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat
    12001 caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa
    12061 agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga
    12121 tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat
    12181 gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct
    12241 gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg
    12301 aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt
    12361 ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct
    12421 actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac
    12481 acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg
    12541 gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat
    12601 gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt
    12661 ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg
    12721 aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc
    12781 aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac
    12841 gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc
    12901 aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa
    12961 tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca
    13021 caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa
    13081 gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac
    13141 caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct
    13201 acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa
    13261 ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa
    13321 ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca
    13381 ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt
    13441 agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc
    13501 tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa
    13561 gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg
    13621 tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt
    13681 tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc
    13741 gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt
    13801 agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac
    13861 caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt
    13921 tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca
    13981 taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc
    14041 agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac
    14101 actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca
    14161 agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat
    14221 tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg
    14281 ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga
    14341 tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga
    14401 agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa
    14461 aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa
    14521 cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat
    14581 caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt
    14641 aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga
    14701 aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta
    14761 ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa
    14821 tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg
    14881 tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt
    14941 aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt
    15001 gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat
    15061 ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg
    15121 tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg
    15181 cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg
    15241 ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag
    15301 gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa
    15361 ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt
    15421 ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg
    15481 cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg
    15541 ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa
    15601 gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt
    15661 tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc
    15721 aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact
    15781 caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga
    15841 ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa
    15901 tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg
    15961 gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat
    16021 ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc
    16081 ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag
    16141 caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga
    16201 tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga
    16261 tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat
    16321 gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct
    16381 aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct
    16441 agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc
    16501 atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct
    16561 attttctctg attcacgcag tagcaaca
//
LOCUS       DACCPY010000227        11909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.091, whole genome shotgun sequence.
ACCESSION   DACCPY010000227 DACCPY010000000
VERSION     DACCPY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.091"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(209..1150)
                     /locus_tag="IVO69_002839"
     CDS             complement(209..1150)
                     /locus_tag="IVO69_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7401547.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1276..2397
                     /locus_tag="IVO69_002840"
     CDS             1276..2397
                     /locus_tag="IVO69_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7401548.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2655..4157
                     /locus_tag="IVO69_002841"
     CDS             2655..4157
                     /locus_tag="IVO69_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7401549.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4224..4751
                     /locus_tag="IVO69_002842"
     CDS             4224..4751
                     /locus_tag="IVO69_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7401550.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4962..6023
                     /locus_tag="IVO69_002843"
     CDS             4962..6023
                     /locus_tag="IVO69_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7401551.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6026..6922
                     /gene="murQ"
                     /locus_tag="IVO69_002844"
     CDS             6026..6922
                     /gene="murQ"
                     /locus_tag="IVO69_002844"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7401552.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6936..8918
                     /locus_tag="IVO69_002845"
     CDS             6936..8918
                     /locus_tag="IVO69_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7401553.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8983..9429)
                     /gene="nrdI"
                     /locus_tag="IVO69_002846"
     CDS             complement(8983..9429)
                     /gene="nrdI"
                     /locus_tag="IVO69_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7401554.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            9640..11064
                     /locus_tag="IVO69_002847"
     CDS             9640..11064
                     /locus_tag="IVO69_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7401555.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11245..>11909)
                     /locus_tag="IVO69_002848"
     CDS             complement(11245..>11909)
                     /locus_tag="IVO69_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7401556.1"
                     /translation="KYTTIIDPVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLK
                     NLLYRKYSRGEILVSKWITLVIYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTL
                     IQSLFTYTLGSYIGLWLILSLVLMIACFINSSGASISAGIVFYFASSIISGILIALIQ
                     KWEWIKWNPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRK
                     NV"
BASE COUNT         3804 a         2106 c         2593 g         3406 t
ORIGIN      
        1 acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa aacaaataaa
       61 aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct tatggcatca
      121 tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa ccagctgctg
      181 attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
      241 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
      301 gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
      361 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
      421 ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa gcaaattcag
      481 atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca gcaatattac
      541 aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg
      601 aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag acttctacaa
      661 ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
      721 ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
      781 agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta
      841 aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac atatcatcta
      901 attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt
      961 cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca
     1021 acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag aggacctcat
     1081 ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc ccagctattg
     1141 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaat
     1201 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     1261 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     1321 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     1381 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
     1441 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
     1501 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
     1561 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     1621 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     1681 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     1741 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     1801 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     1861 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     1921 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     1981 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     2041 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     2101 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     2161 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     2221 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
     2281 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     2341 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     2401 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     2461 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     2521 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     2581 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     2641 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     2701 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     2761 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     2821 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     2881 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     2941 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     3001 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     3061 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     3121 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     3181 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     3241 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     3301 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     3361 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     3421 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     3481 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     3541 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     3601 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     3661 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     3721 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     3781 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     3841 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     3901 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     3961 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     4021 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     4081 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     4141 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     4201 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     4261 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     4321 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     4381 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     4441 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     4501 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     4561 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     4621 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     4681 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     4741 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     4801 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     4861 aatgaaattt aattatttta ataaaattat tgaaattttg tttctttaga tatatactct
     4921 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     4981 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     5041 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     5101 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     5161 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     5221 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     5281 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     5341 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     5401 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     5461 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     5521 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     5581 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     5641 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     5701 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     5761 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     5821 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     5881 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     5941 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     6001 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     6061 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     6121 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     6181 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     6241 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     6301 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     6361 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     6421 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     6481 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     6541 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     6601 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     6661 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     6721 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
     6781 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
     6841 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
     6901 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
     6961 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
     7021 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
     7081 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
     7141 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
     7201 taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag
     7261 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
     7321 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
     7381 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
     7441 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
     7501 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
     7561 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
     7621 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
     7681 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
     7741 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
     7801 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
     7861 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
     7921 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
     7981 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
     8041 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
     8101 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
     8161 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
     8221 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
     8281 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
     8341 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
     8401 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
     8461 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
     8521 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
     8581 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
     8641 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
     8701 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
     8761 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
     8821 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
     8881 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
     8941 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
     9001 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
     9061 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
     9121 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
     9181 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
     9241 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
     9301 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
     9361 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
     9421 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
     9481 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
     9541 agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata
     9601 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
     9661 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
     9721 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
     9781 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
     9841 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
     9901 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
     9961 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
    10021 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
    10081 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
    10141 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
    10201 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
    10261 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
    10321 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
    10381 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
    10441 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
    10501 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
    10561 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
    10621 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
    10681 aatgtctttt tactttcatt attattcact gacagtgctt ataactttgc ttacacactt
    10741 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
    10801 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
    10861 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
    10921 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
    10981 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
    11041 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
    11101 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
    11161 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
    11221 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
    11281 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
    11341 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
    11401 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
    11461 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcgccggaac tattgataaa
    11521 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
    11581 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
    11641 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
    11701 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
    11761 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
    11821 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
    11881 aactggatca atgatcgtcg tgtattttt
//
LOCUS       DACCPY010000228         9607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.103, whole genome shotgun sequence.
ACCESSION   DACCPY010000228 DACCPY010000000
VERSION     DACCPY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.103"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            31..207
                     /locus_tag="IVO69_002849"
     CDS             31..207
                     /locus_tag="IVO69_002849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401557.1"
                     /translation="MHPAPFRRKAQRLTACGPQGERKALYPGAAQRGQDRTGQAATGA
                     GKIKGIGGRVNAEV"
     gene            194..1138
                     /locus_tag="IVO69_002850"
     CDS             194..1138
                     /locus_tag="IVO69_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401558.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            1142..1345
                     /locus_tag="IVO69_002851"
     CDS             1142..1345
                     /locus_tag="IVO69_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401559.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(1645..1881)
                     /locus_tag="IVO69_002852"
     CDS             complement(1645..1881)
                     /locus_tag="IVO69_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401560.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(2064..2435)
                     /locus_tag="IVO69_002853"
     CDS             complement(2064..2435)
                     /locus_tag="IVO69_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401561.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(2432..3760)
                     /locus_tag="IVO69_002854"
     CDS             complement(2432..3760)
                     /locus_tag="IVO69_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7401562.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(3721..4050)
                     /locus_tag="IVO69_002855"
     CDS             complement(3721..4050)
                     /locus_tag="IVO69_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7401563.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(4308..4679)
                     /locus_tag="IVO69_002856"
     CDS             complement(4308..4679)
                     /locus_tag="IVO69_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401564.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            4824..5009
                     /locus_tag="IVO69_002857"
     CDS             4824..5009
                     /locus_tag="IVO69_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7401565.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            5952..6611
                     /gene="vanR"
                     /locus_tag="IVO69_002858"
     CDS             5952..6611
                     /gene="vanR"
                     /locus_tag="IVO69_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP7401566.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTYEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            6614..7957
                     /gene="vanS"
                     /locus_tag="IVO69_002859"
     CDS             6614..7957
                     /gene="vanS"
                     /locus_tag="IVO69_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7401567.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            8128..8937
                     /gene="vanY"
                     /locus_tag="IVO69_002860"
     CDS             8128..8937
                     /gene="vanY"
                     /locus_tag="IVO69_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7401568.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            8955..>9607
                     /gene="vanW"
                     /locus_tag="IVO69_002861"
     CDS             8955..>9607
                     /gene="vanW"
                     /locus_tag="IVO69_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein VanW"
                     /protein_id="HAP7401569.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     "
BASE COUNT         2476 a         2304 c         2448 g         2379 t
ORIGIN      
        1 caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg gcgaaaagcc
       61 cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc gggcgcagct
      121 caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat caaagggatt
      181 ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat
      241 catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag acattatcag
      301 gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt cccggccctc
      361 cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac agtacaacac
      421 atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg tggagctgac
      481 cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc cgcagcattt
      541 ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt atgagcatgg
      601 cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg tattgggaaa
      661 ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg cgctacggac
      721 ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg tcaccgcttt
      781 gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga aagatctgtc
      841 cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc actttgcggc
      901 gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata aatccctgtg
      961 ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg acgagcgccg
     1021 ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg acagcaaaca
     1081 ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg aggtataagg
     1141 aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca atgcagaccg
     1201 gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg atatgcgggc
     1261 gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact atgacggcca
     1321 gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg gccatgtggt
     1381 cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac ccggtattgt
     1441 cggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg
     1501 gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct gaaacagata
     1561 tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt
     1621 aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg gcaatgcttc
     1681 cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct tctttttgcc
     1741 gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa
     1801 cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt catagtccag
     1861 cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc tatgatacca
     1921 tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt
     1981 agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga ccatgccgga
     2041 aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca gtatcttttc
     2101 cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc gcagtatttg
     2161 gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc
     2221 cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc ggccttaaac
     2281 cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc
     2341 agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt ccgggccttg
     2401 cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat tcctttcccg
     2461 gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc tgcatctgct
     2521 ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat cgcttgtact
     2581 cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt agggcctttg
     2641 ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt gccttatccg
     2701 ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa atattcttta
     2761 tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg gcggcctcgt
     2821 cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg tctatcccat
     2881 gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc
     2941 tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa
     3001 tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc tctttgatgg
     3061 cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag
     3121 cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc atgagccgca
     3181 ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct ttgagcttcg
     3241 ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg cccttactct
     3301 ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga
     3361 tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata tccacggcac
     3421 agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc acatagggat
     3481 atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc tgttccggcg
     3541 tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg cccttttggt
     3601 atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag caggcaaagg
     3661 aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca agggcttttt
     3721 tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca gcgccccctt
     3781 tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct ttgcaatctg
     3841 attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc
     3901 catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa agttcttcgt
     3961 atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca cagggacgcg
     4021 cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt caggggtttg
     4081 gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag gccgtttttc
     4141 cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa caagacgttt
     4201 ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag
     4261 aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta cacatcctcc
     4321 gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc
     4381 ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg cagttcgctt
     4441 tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca ggctttaata
     4501 agctgtatga caaccggcag actgggaatg gccccgtcgt tttcgatgtt ggcaagatag
     4561 cgccagtcaa tatttatttt ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg
     4621 gccgctttca catccgcgcc gaaagtttca aagccgggac aatcttcaat attcgccata
     4681 tcgcacctcc cacttacatt gtatatttca tactctgccc gtggaatgtt ggtatatgca
     4741 cctttcttaa agtttatatt tcacattttg tttcttttat tcttcgagca aaagaattat
     4801 aatgtaccct gtggaggtgt actatggact acatgacgtt gaaagaggca ggcgagaaat
     4861 ggggtgtgcc cccacgatgg ataaactact attgtgctgc gggacgcatc cccggcgctg
     4921 taaagatggc gacgatctgg ctcattccca aaactgcgga aaagccgatt gacaggcgta
     4981 caaagaaagg aaaggcggtt actgaatagt ataatgaaac cggcgcattt tggatatggt
     5041 tttgaagaga gagaaagcat ggtattttgt cacggaaaaa ctataagatg atagatggca
     5101 ggctgctcca gaccgataaa aagtattctc atctgaaaca gaaagaaaag atttacacat
     5161 ggatgtatga agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg aaacaaagac
     5221 gaggaagctg ctgctgtatc agccgtatat ggtagaattg aaaaggccgg catctggatt
     5281 ctttatggtg aagtctccag acattggata actgctggtc taatgcgcct ttgagtatgg
     5341 ggtgattgat tttaataccg gggaggtacg gctttttgag gaaaatattc tgggatttac
     5401 atttggcgat tattacattt cctgtaactg caaggacacg gatgatgaag atatgaggaa
     5461 tatagacttg ctgattttct actgtcttga acggtattag gactgaatct gctgcgcgga
     5521 gtgcggcaag agcgaccgga gggagtacac acaggccctg cctgtgtgaa aagccatacc
     5581 gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg tgtccccaac
     5641 aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc agaatatccg taaaggatat
     5701 tctgatccat tgcttgccag tattatcggg atgttttgac aaccgctgta atcgaaacat
     5761 cccaaacatt aagaaggtgt taattattat tatcttgatt taattcgttt aaaccaataa
     5821 tatagataca tacaccatga aaagaagcga gtatcccaat gacggatgat taaaatccga
     5881 aaagaaatcg ctttgaaact acagggaaac tacagactgt tatgttatct tcttaaaatg
     5941 gagggatttt tatgtcgata cgaattctac ttgtcgagga tgatgatcat atctgtaata
     6001 cagtaagggc gtttctggct gaggcaggat atcaggtgga tgcctgcaca gatggaaatg
     6061 aggcatacac caagttttac gaaaacactt atcaactggt tattcttgat attatgctgc
     6121 ccggtatgaa cgggcatgaa cttttgcgtg aatttcgtgc gaaaaatgat actcccattc
     6181 tgatgatgac agccctgtcg gatgacgaaa accaaatccg ggcgtttgat gcagaggcag
     6241 acgactatgt aacaaagcca ttcaagatgc agattttact aaagcgggtg gaagccctgc
     6301 tgcggcgcag cggtgcgctg gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg
     6361 aggatttcac ggtactttgt gacggtacag agctgcccct gacacgaaaa gaatttgaaa
     6421 tccttttgct gctggtgcag aacaaaggca gaaccttaac gtatgaaatc attttgtcac
     6481 gtatatgggg atatgacttt gagggtgatg gcagcacagt ccacactcat atcaaaaatt
     6541 tgcgggccaa gctgccggaa aatatcatca aaaccattcg cggtgtagga taccgattgg
     6601 aggaatcata ataatggaaa gaaaagggat tttcattaag gttttttcct atacgatcat
     6661 tgtcctgcta ctgcttgtcg gtgtaacagc aacactgttt gcacagcaat ttgtgtctta
     6721 tttcagagtg atggaattac agcaaacagt aaaatcctat cagccattgg tggaactgat
     6781 tcagaatagc gataggcttg atattcaaga ggtggcaggg ctgtttcact acaataacca
     6841 atcctttgag ttttatattg aagataaaga gggaagcgta ctctatgcca caccaaatgc
     6901 caatacatca aatagtttta gatccgactt tctttatgtg gtacatagag atgataatat
     6961 ttcgattgtt gctcaaagca aggcaggtgt gggattgctt tatcaagggc tgacaattcg
     7021 gggaattgtt atgattgcga taatggttgt attcagcctt ttatgcgcgt atatctttgc
     7081 gcggcaaatg acaacgccga tcaaagcctt agcggacagt gcgaataaaa tggcaaacct
     7141 gaaagatgta ccgccgccgc tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat
     7201 gcattccatg tatgtcaggc tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag
     7261 ggaacatgag ttggaggaaa cacagcgata tttctttgcg gcagcttctc atgagctaaa
     7321 aacgcccatc gcggctacaa gcgttctgtt ggagggaatg cttgaaaata tcggtgacta
     7381 caaagatcat tctaagtatc tgcgcgaatg catcaaaatg atggataggc agggcaaaat
     7441 catttccgaa atactggagc ttgtcagcct gaatgatggg agaatcgtac ccatagctga
     7501 accgttggac atagggcgca cggttgccga gttgctgccc gattttcaaa ccttggcaga
     7561 ggcaaacaac cagcggttcg tcacagatat tccagccggg caaattgtcc tgtccgatcc
     7621 gaggctgctc caaaaggcac tatccaatgt catattgaat gcagttcaga acacgccgca
     7681 gggaggcgag gtacggatat ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt
     7741 gaacatgggc gttcacattg atgatactgc gcttccaagg ctgttcaccc cattctatcg
     7801 cattgatcag gcgcgaagca gaaaaagtgg gcgaagcggt ttaggacttg ccatcgtaca
     7861 aaaaacgctg gatgccatga gcctccagta tgcgctggaa aacacctcgg atggcgtttt
     7921 gttctggctg gatttaccgc tcacatcaac attgtaaata tttaaaactt aaatgatttt
     7981 gaccgacagg tataaccctg ccggtctttt tttgctgcta caggaaaact acagactggc
     8041 tacagggaaa gtacagatgc gcttgccata ataacaatcg taccagccac aaatcgtagt
     8101 tttattgcaa aggaggcatt cgatcaaatg gaaaaaaaaa gcaactatca ttccaatgcg
     8161 gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc
     8221 attatttcgt tcacagtctg cacgctgttt ttggggtgga gactggcttc cgtattggag
     8281 gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag
     8341 aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc
     8401 ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa
     8461 ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat
     8521 gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa
     8581 cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca
     8641 caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt
     8701 ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga
     8761 tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca
     8821 gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc
     8881 acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa
     8941 aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa
     9001 tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct
     9061 atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat
     9121 tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa
     9181 agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct
     9241 ggtggctggt acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca
     9301 atggtaaact caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt
     9361 ggatgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt
     9421 ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga
     9481 ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg
     9541 atgagaaaat catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta
     9601 caaatgg
//
LOCUS       DACCPY010000229         5883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.131, whole genome shotgun sequence.
ACCESSION   DACCPY010000229 DACCPY010000000
VERSION     DACCPY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.131"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..175
                     /locus_tag="IVO69_002862"
     CDS             <1..175
                     /locus_tag="IVO69_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7401570.1"
                     /translation="FTPFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDG
                     VLFWLDLPLTSTL"
     gene            346..1155
                     /gene="vanY"
                     /locus_tag="IVO69_002863"
     CDS             346..1155
                     /gene="vanY"
                     /locus_tag="IVO69_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7401571.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1173..2000
                     /gene="vanW"
                     /locus_tag="IVO69_002864"
     CDS             1173..2000
                     /gene="vanW"
                     /locus_tag="IVO69_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7401572.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1997..2968
                     /locus_tag="IVO69_002865"
     CDS             1997..2968
                     /locus_tag="IVO69_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7401573.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2961..3989
                     /gene="vanB"
                     /locus_tag="IVO69_002866"
     CDS             2961..3989
                     /gene="vanB"
                     /locus_tag="IVO69_002866"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7401574.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            3995..4603
                     /locus_tag="IVO69_002867"
     CDS             3995..4603
                     /locus_tag="IVO69_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7401575.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            5191..5622
                     /locus_tag="IVO69_002868"
     CDS             5191..5622
                     /locus_tag="IVO69_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7401576.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            5629..5862
                     /locus_tag="IVO69_002869"
     CDS             5629..5862
                     /locus_tag="IVO69_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7401577.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
BASE COUNT         1617 a         1278 c         1578 g         1410 t
ORIGIN      
        1 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcggttt
       61 aggacttgcc atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa
      121 cacctcggat ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt
      181 taaaacttaa atgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca
      241 ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta
      301 ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaaaaagc
      361 aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg
      421 gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga
      481 ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc
      541 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
      601 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
      661 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
      721 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
      781 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
      841 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
      901 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
      961 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
     1021 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
     1081 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
     1141 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
     1201 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
     1261 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
     1321 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
     1381 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
     1441 gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag
     1501 acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga
     1561 tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag
     1621 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
     1681 ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
     1741 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc
     1801 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
     1861 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
     1921 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
     1981 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
     2041 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
     2101 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg
     2161 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
     2221 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
     2281 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
     2341 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
     2401 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
     2461 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
     2521 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
     2581 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
     2641 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc gcggagcgct
     2701 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
     2761 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
     2821 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
     2881 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
     2941 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
     3001 cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg
     3061 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
     3121 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
     3181 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
     3241 tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg tttgtattgt
     3301 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
     3361 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa atgattgata
     3421 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
     3481 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
     3541 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
     3601 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
     3661 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
     3721 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
     3781 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
     3841 tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt tttacatcgt
     3901 acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca
     3961 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
     4021 tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg
     4081 aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc
     4141 tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg
     4201 atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa
     4261 gctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta
     4321 tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct
     4381 ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca
     4441 tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat
     4501 catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga
     4561 tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg
     4621 tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt
     4681 ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga
     4741 aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg
     4801 cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata
     4861 ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa
     4921 aaaagccttg cccctccaca gggctattcc ggttatgagt atgtactata ccatgtaaat
     4981 cagcagcata gcaaagcatt ttctcgcccg gtaggtttgc gacctgccgg gcgatttttg
     5041 tcgtcttttg cggcccggct gtccccaaaa aaattttttg agaaattttc aaaaaggagg
     5101 tatttagcgg gtagctggcg gccttgtcaa agtcttgtta gcggaaaggg agagaaccac
     5161 ctaagtcccc cacagaaagg gggtgagaag atggatgcta tcccccgcga ctatgaagcg
     5221 cggtgcatgt tcgatgcttt ttgcaagacg gtactgcgta acgaggccaa aagctacttg
     5281 gctgaaatga agcgccgccg tgaccgtgaa gtgtcactaa gctctttgtc acaggcagat
     5341 ttggacaagc tctgtacagt agatcactac cccagcgaca gctttacgtt ttcgtcgcat
     5401 ggctatgacc tacatatcaa caatgagctt gttgccgaag cctttgccgc gctgcccagc
     5461 atggagcaga gtattttaat cctgcactgt gttctggata tggcagatgg tgagatcggc
     5521 ggccttgtgg gaatgtcccg gagtgccgtt cagcggcaca ggacaaatac cttatcggag
     5581 ctgcggaaac agctaaaggc gttgatgccg aagggaggtt agacgcggat gaagaacccg
     5641 aactacaagg gcattgagct tttgcccctt tcggtgatcg aggccgcccg cgctggggat
     5701 gccggagccg tagagcgcgt tttgcagtat tacagcggtt acatgaacaa gctgtgtacc
     5761 cggacgctct atgacgaaaa cggctatccg catgtgtgtc tggacgaggc catgaagcgc
     5821 cgtctggaaa tcaagctcat ccacgctatc gtcacgaact gacgaaccgg ccccggcagc
     5881 atg
//
LOCUS       DACCPY010000230         4768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.137, whole genome shotgun sequence.
ACCESSION   DACCPY010000230 DACCPY010000000
VERSION     DACCPY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.137"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            273..384
                     /locus_tag="IVO69_002870"
                     /pseudo
     CDS             273..384
                     /locus_tag="IVO69_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            405..488
                     /locus_tag="IVO69_002871"
     CDS             405..488
                     /locus_tag="IVO69_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7401578.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYVDK"
     gene            613..1350
                     /gene="erm(B)"
                     /locus_tag="IVO69_002872"
     CDS             613..1350
                     /gene="erm(B)"
                     /locus_tag="IVO69_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7401579.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1822..2070
                     /locus_tag="IVO69_002873"
     CDS             1822..2070
                     /locus_tag="IVO69_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7401580.1"
                     /translation="MELAFRSRLRQMRGNKTRKQFAEEIGMKESNYLKIELGKSNPTV
                     KTLERIAKLTNSTLVIDLIPNDSEQLELQLDSEISKKE"
     gene            2462..3952
                     /locus_tag="IVO69_002874"
     CDS             2462..3952
                     /locus_tag="IVO69_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7401581.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKRFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            4484..>4768
                     /locus_tag="IVO69_002875"
     CDS             4484..>4768
                     /locus_tag="IVO69_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7401582.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMN"
BASE COUNT         1728 a          718 c          882 g         1440 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa atttcgctgt tattttgtac
       61 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
      121 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
      181 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
      241 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
      301 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
      361 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
      421 tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgtag
      481 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
      541 taactaaaat tacaaacaaa tcgtttaact tctgtattta tttatagatg taatcacttc
      601 aggagtgatt acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
      661 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
      721 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
      781 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaacattcgt
      841 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
      901 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
      961 agccatgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     1021 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     1081 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     1141 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     1201 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     1261 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     1321 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     1381 gttacacgtt actaaaggga atgtagataa attattaggt atactactga cagcttccaa
     1441 ggagctaaag aggtccctag cgcctacggg gaatttgtat cgataaggaa tagatttaaa
     1501 aatttcgctg ttattttgta caataaggat aaatttgaat ggtaccataa acgaccgttt
     1561 atggtaccat ttcattttcc tgctttttct aaatggggtc ccgagcgctt agaatcgctt
     1621 taggaaacac gatccagtcc aataatcgtc gataaaaact tttgaaaaag gttggtgaaa
     1681 ttacctactt ttggaataat cacaaatcac aagtgattaa tcacaaatca caagtgatta
     1741 atcacttgtt tattaagata ttaaaagcta taatttaaaa taaagcgtga attttattac
     1801 acaaaaagag gggggagaaa cttggagcta gcattcagaa gtcgtttaag acaaatgaga
     1861 ggaaataaaa cacgtaaaca gtttgctgaa gaaattggaa tgaaagaatc taattactta
     1921 aaaattgagt taggaaaatc aaacccgact gtaaaaacac tggaacggat tgcaaaactt
     1981 acaaattcaa ccctggtcat tgatttgatc cctaacgatt cggaacagct agaattacaa
     2041 ctagatagcg aaataagcaa aaaagagtaa acgtaatatt tttacacttt ttaattgctt
     2101 atttttttta aaagcggtat actagatata acgaaacaac gaactgaata aagaatacaa
     2161 aaaaagagcc acgaccaact tattttccgc caagaaaata agttgcgagc cttaattgat
     2221 taccaccaat caattaaaga agtcgagacc caaaatttgg taaagtattt aattacttta
     2281 ttaatcagat acttaaatat ctgtaaaccc attatatcgg gtttttgagg gaatttcaag
     2341 tctttaagaa gataccaggc aatcaattaa gaaaaactta gttgattgcc ttttttgttg
     2401 tgattcaact ttgatcgtag cttctaacta attaattttc gtaagaaagg agaacagctg
     2461 aatgaatatc ccttttgttg tagaaactgt gcttcatgac ggcttgttaa agtacaaatt
     2521 taaaaatagt aaaattcgct caatcactac caagccaggt aaaagcaaag gggctatttt
     2581 tgcgtatcgc tcaaagaaaa gcatgattgg cggacgtggc gttgttctga cttccgaaga
     2641 agcgattcac gaaaatcaag atacatttac gcattggaca ccaaacgttt atcgttatgg
     2701 tacgtatgca gatgaaaacc gttcatacac gaaaggtcat tcagaaaata atttaagaca
     2761 aatcaatacc ttctttattg attttgatat tcacacggca aaagaaacaa tttcagcaag
     2821 cgatatttta acaacagcga ttgatttagg ttttatgcct acgttgatta tcaaatctga
     2881 taaaggttat caagcatatt ttgttttaga aacgccagtc tatgtgactt caaaatcaga
     2941 atttaaatct gtcaaagcag ccaaaataat ctcgcaaaat atccgagaat attttggaaa
     3001 gtctttgcca gttgatctaa cgtgcaatca ttttgggatt gctcgtatac caagaacgga
     3061 caatgtagaa ttttttgatc ccaattaccg ttattctttc aaagaatggc aagattggtc
     3121 tttcaaacaa acagataata agcgctttac tcgttcaagt ctaacggttt taagcggtac
     3181 agaaggcaaa aaacaagtag atgaaccctg gtttaatctc ttattgcacg aaacgaaatt
     3241 ttcaggagaa aagggtttaa tagggcgtaa taacgtcatg tttaccctct ctttagccta
     3301 ctttagttca ggctattcaa tcgaaacgtg cgaatataat atgtttgagt ttaataatcg
     3361 actagatcaa cccttagaag aaaaagaagt aatcaaaatt gttagaagtg cctattcaga
     3421 aaactatcaa ggggctaata gggaatacat taccattctt tgcaaagctt gggtatcaag
     3481 tgatttaacc agtaaagatt tatttgtccg tcaagggtgg tttaaattca agaaaaaaag
     3541 aagcgaacgt caacgtgttc atttgtcaga atggaaagaa gatttaatgg cttatattag
     3601 cgaaaaaagc gatgtataca agccttattt agtgacgacc aaaaaagaga ttagagaagc
     3661 gctaggcatt cctgagcgga cattagataa attgctgaag gtactgaagg cgaatcagga
     3721 aattttcttt aagattaaac caggaagaaa tggtggcatt caacttgcta gtgttaaatc
     3781 attgttgcta tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct
     3841 gacaaattct tttgacttag agcatacatt cattcaagag actttaaaca agctagcaga
     3901 acgccctaaa acggacacac aactcgattt gtttagctat gatacaggct gaaaataaaa
     3961 cccgcactat gccattacat ttatatctat gatacgtgtt tgttttttct ttgctgttta
     4021 gtgaatgatt agcagaaata tagagagtaa gattttaatt aattattagg gggagaagga
     4081 gagagtagcc cgaaaacttt tagttggctt ggactgaacg aagtgaggga aaggctacta
     4141 aaacgtcgag gggcagtgag agcgaagcga acacttgatt ttttaagttg ctatcattta
     4201 taggtcaata gagtatactt atttgtccta atatggtttt agcagtataa ttgacttggt
     4261 gaataggtca tttaagttga gcataatagg aggagtaaaa tgaaaaaatt tatttatcga
     4321 gttttagaaa atgacgaagt ggtggctatt tttaatgagc aacaatatgc gcaagatttt
     4381 atcgcttacg aaaagacaat ttctgataag caatttgaaa ttgaaaaagt agatatagct
     4441 gattggttat tgcaaccgag agaattttag aggttggttg aaaatggcga aaattggtta
     4501 tgcacgtgtc agtagcaaag aacagaactt agatcggcaa ttacaagcgt tacagggcgt
     4561 ttctaaggtc ttttcagaca aattaagcgg tcaatcggtc gaacgcccac aattacaagc
     4621 tatgcttaac tatattcgtg aaggggatat agttgttgtt actgaattag atcgattagg
     4681 acgaaataat aaagaattaa cagaattgat gaatcaaatt caaattaagg gggcaaccct
     4741 ggaagtctta aatttaccct caatgaat
//
LOCUS       DACCPY010000231         4366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.140, whole genome shotgun sequence.
ACCESSION   DACCPY010000231 DACCPY010000000
VERSION     DACCPY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.140"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            215..265
                     /locus_tag="IVO69_002876"
                     /pseudo
     CDS             215..265
                     /locus_tag="IVO69_002876"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            299..1675
                     /gene="tet(L)"
                     /locus_tag="IVO69_002877"
     CDS             299..1675
                     /gene="tet(L)"
                     /locus_tag="IVO69_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7401583.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTALYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FPILILARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTIVSSSLKQKEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNMLFLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2239..3501
                     /locus_tag="IVO69_002878"
     CDS             2239..3501
                     /locus_tag="IVO69_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7401584.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3746..4285
                     /locus_tag="IVO69_002879"
     CDS             3746..4285
                     /locus_tag="IVO69_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7401585.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHS"
BASE COUNT         1482 a          623 c          890 g         1371 t
ORIGIN      
        1 aaaaggatat caggttacca ctggcgaacc tgtttgccag ccccgtcgtc caaatagtcg
       61 gatagataaa gtacgatata tgttcaataa aataacttag cacattttat attgttatat
      121 aaatgtggct tcttgttaaa cgaaacgaga tgaaatattt tttaataaag atttgaatta
      181 aagtgtaaag gaggaattaa ttatgaagta aaatgaatgt aacagggttc aattaaaaga
      241 gggaagcgta tcattaaccc tataaactac gtctgccctc attattggag ggtgaaatgt
      301 gaatacatcc tattcacaat cgaatttacg acacaaccaa attttaattt ggctttgcat
      361 tttatctttt tttagcgtat taaatgaaat ggttttgaac gtctcattac ctgatattgc
      421 aaatgatttt aataaaccac cggcgagtac aaactgggtg aacacagcct ttatgttaac
      481 cttttctatt ggaacagctc tatatggaaa gctatctgat cagctaggca ttaaaaggtt
      541 actcctattt gggattataa taaattgctt cggatcggta attgggtttg ttggacattc
      601 tttctttccc atacttattc tggctcgatt tattcaagga gctggtgcag ctgcatttcc
      661 agcactcgtg atggttgtag ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt
      721 tggtcttatt ggatcgatag tagccatggg agaaggagtc ggtccagcga ttggtggaat
      781 gatagcccat tatattcatt ggtcctatct tctactcatt cctatgataa caattatcac
      841 tgttccgttt cttatgaaat tattaaagaa agaagtaagg ataaaaggtc attttgatat
      901 caaaggaatt atactaatgt ctgtaggcat tgtatttttt atgttgttta caacatcata
      961 tagcatttct tttcttatcg ttagcgtgct gtcattcctg atatttgtaa aacatatcag
     1021 gaaagtaaca gatccttttg ttgatcccgg attagggaaa aatatacctt ttatgattgg
     1081 agttctttgt gggggaatta tatttggaac agtagcaggg tttgtctcta tggttcctta
     1141 tatgatgaaa gatgttcacc agctaagtac tgccgaaatc ggaagtgtaa ttattttccc
     1201 tggaacaatg agtgtcatta ttttcggcta cattggtggg atacttgttg atagaagagg
     1261 tcctttatac gtgttaaaca tcggagttac atttctttct gttagctttt taactgctgc
     1321 ctttttttta gaagtaacac catggttact gacaattata ttagtttttg tttttggtgg
     1381 gctgtcattc accaaaacag ttatatctac aattgtttca agtagtttga aacaaaagga
     1441 agctggtgcc ggaatgagtt tgcttaattt taccagcttt ttatcagagg gaacaggtat
     1501 tgcaattgtt ggtggtttat tatctgtacg cttgctgaat cagaagttgc tacctatgga
     1561 ccttgatcaa tccacttact tgtatagtaa tatgctattc cttttttcag gaatcattgt
     1621 cattagttgg ctggttacct tgaatgtata taaacattct caaagggatt tctaaatcgt
     1681 taagggatca actttgggag agagttcaaa attgatcctt tttttataac aggaattcaa
     1741 atctttttgt tccattaaag ggcgcgattg ctgaataaaa gatacgagag acctctcttg
     1801 tatctttttt attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa
     1861 ttttattctt gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg
     1921 gcaagggttt aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct
     1981 tgctgatttt taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag
     2041 gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg
     2101 gtcggcactg ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt
     2161 atactttact tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta
     2221 aaaaggagcg atttacatat gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct
     2281 ggactaaaag gcatgcaatt tcataatcaa agagagcgaa aaagtagaac gaatgatgat
     2341 attgaccatg agcgaacacg tgaaaattat gatttgaaaa atgataaaaa tattgattac
     2401 aacgaacgtg tcaaagaaat tattgaatca caaaaaacag gtacaagaaa aacgaggaaa
     2461 gatgctgttc ttgtaaatga gttgctagta acatctgacc gagatttttt tgagcaactg
     2521 gatccaggag aacaaaaacg attttttgag gaaagttata aattattttc cgaacgatat
     2581 ggcaagcaaa atattgctta tgcaacagtt cataatgatg agcaaacccc tcacatgcat
     2641 ttaggtgttg tgcctatgcg tgatggaaaa ctgcaaggaa aaaatgtgtt taatcgtcaa
     2701 gaactgttat ggctacaaga taaattcccc gagcatatga aaaaacaggg ttttgagttg
     2761 aagcgtggtg aacgtggctc tgaccgtaaa catattgaga cagctaaatt taaaaaacaa
     2821 actttggaaa aagagattga ttttctagaa aaaaatttag cagttaaaaa agatgaatgg
     2881 actgcttata gcgataaagt taaatcagat ttagaagtac cagcgaaacg acacatgaaa
     2941 agtgttgaag tgccaacggg tgaaaagtcc atgtttggtt tgggaaaaga aataatgaaa
     3001 acagaaaaga aaccaaccaa aaatgttgtt atatcggagc gtgattataa aaacttagtg
     3061 actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat gagtactgat
     3121 atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga aaaatatagt
     3181 ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac
     3241 aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc
     3301 actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag
     3361 gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa
     3421 cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga
     3481 gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg
     3541 ttttgaactt gttctttctt atcttgatac atatagaaat aacgtcattt ttattttagt
     3601 tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa
     3661 aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac aaataactaa
     3721 atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa
     3781 atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa
     3841 aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag
     3901 agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa
     3961 gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc
     4021 aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac
     4081 agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa
     4141 taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa
     4201 taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata aagataattc
     4261 ttataatcag cacatgcatt cttaatcact tggatatatt ctttttgttt taacgcttca
     4321 ataatacttg ctcttgtcgc ttctgttccg atcccctcga cttctt
//
LOCUS       DACCPY010000232         4311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.141, whole genome shotgun sequence.
ACCESSION   DACCPY010000232 DACCPY010000000
VERSION     DACCPY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.141"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..338
                     /locus_tag="IVO69_002880"
     CDS             <1..338
                     /locus_tag="IVO69_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7401586.1"
                     /translation="WPFYFYTIIRTLSKYYIAGHVFRIISCLNQVLFACNNAYCINEK
                     KAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNE
                     ESSDERKQI"
     gene            319..1053
                     /locus_tag="IVO69_002881"
     CDS             319..1053
                     /locus_tag="IVO69_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7401587.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1086..1949
                     /locus_tag="IVO69_002882"
     CDS             1086..1949
                     /locus_tag="IVO69_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7401588.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            1993..2520
                     /locus_tag="IVO69_002883"
     CDS             1993..2520
                     /locus_tag="IVO69_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7401589.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            2748..4067
                     /locus_tag="IVO69_002884"
     CDS             2748..4067
                     /locus_tag="IVO69_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HAP7401590.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
BASE COUNT         1459 a          681 c          871 g         1300 t
ORIGIN      
        1 tatggccttt ttacttttac acaattatac ggactttatc taaatattat attgcaggcc
       61 atgtttttcg tattatttca tgcttaaatc aagtactatt tgcatgtaat aatgcttatt
      121 gcattaacga aaagaaagct ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat
      181 atgccgaaag agttaatcat atttttgaag tactcggtct ttctcttttt gaatgctacg
      241 atatgaccga gaaactttat aaagaagtga atgaaattgt atcggagata aataactttt
      301 taaacgagga gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa
      361 tacagtcaaa tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg
      421 aaaaagatgc tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga
      481 tggcactgta tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct
      541 cataaaatgc tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc
      601 tgtgccatag aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt
      661 gcgtttcatt atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag
      721 gctggtggca atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa
      781 gactggtatt ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat
      841 gagggcaaac ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg
      901 accacatatc taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca
      961 cagccgccag agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg
     1021 atgctgattg tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta
     1081 tgtaaatgag atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg
     1141 aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg
     1201 aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt atatctaatg
     1261 atgactggct taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat
     1321 tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa
     1381 ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa
     1441 taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag
     1501 attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga
     1561 atgtaacacc ttatgttatt aaaggattgt gccgcaaaga gatactgttt gcaatcgatc
     1621 atctgaacca gattctacgg tttgaactac ttaggatgat gtcgtggaag gttgggataa
     1681 agacagaatt ttcattaagt gttgggaaaa attataagta tattaacaaa tacattgatg
     1741 aagatctatg gaatagatta ttatctacat atcgcatgga ttcctatgaa aatatttgga
     1801 agtcattatt tatatgccac caattgttca gggaagtgtc caaagaggta gcagaactac
     1861 tggggtttga ttatccagag tatggtaaga acataacaag atataccgag gacatgtata
     1921 aaaaatatgt tgaaaatgac tatttttaaa gattagcaaa gattattaac cataagcaag
     1981 gggtgtaaat gtatggaaag tactgatgtt ataaatgaaa ttagaggtat attacctcgt
     2041 aactcaattg gaaaatatga tttactaact atttttacac ataaaaccgt ttttgataaa
     2101 attgtaaaag tactatcatt ggattttcaa aataaagtag attatgtggc tgccccagaa
     2161 gcaattggat ggatactagg cactgccata gccaaagaac ttggagtggg gtttattgga
     2221 gtaagaaaag gtgataagct gccatacgca aaagaagaga ttatttcgac acattttacc
     2281 gactattcag gtcaagataa aagctttgaa atatctaaga gttctattgt gaggaacaaa
     2341 agagttttga ttgtagatga ttggatagag acaggttctc aaatgaaggc tttaatagct
     2401 ttgttagaga aattggattg cagcattatt gggttggcta ctattgggat tgatataaat
     2461 gaggttactc aaaaatggat agacagcagt tttgtagctt atattggctc aaacatataa
     2521 taaaccgaaa gaaaatcttg aatcaaaatt ttggatggcg ttaatttaca atacaacgaa
     2581 agatgtttac cctgaataat tcatactttt taattgaaac cgtctgaaac tgcctaacgg
     2641 cagtctgata attacttttt tagtttcacc ccttaagtga tgaaaaaaga accccattct
     2701 gctatggtaa aggtgacgaa accaaccata agaaaggagt tcttctcatg actagcttac
     2761 acaaaaatca agttaaattc aattcaaata tcactatttc acataccggt ggtcgtctat
     2821 caagtgattc ggggttagtc ctggttaaag aattaatgaa taccttcggc ttttcgcact
     2881 tagctaaaca atggctccat attacagatc atcgggctta ttttacccat gataacttag
     2941 cgatattgga acagcttatt atgcaattaa ttgctggtta ctcggcagac tcatcagcta
     3001 atctattaag acgggatcct gtctttcaag ctgtactcgg taaaaaacag ttggcatcac
     3061 aatcctcaat ctcacggttt ctggatcgtt tcaccgagga gaacatcgat caactccaag
     3121 cattaaatca gtcattgatt gataaagcac gtttaattcg caatgacacc gagttaatca
     3181 ttgatgtcga ttccacacat tcggatacct ttggacgcca agaacaaaca gattataatg
     3241 cccattatca aacctacggc tatcacccat tagttgcctt tgatggactg accggagatt
     3301 tcttaaaagc tgaactgcgt tcaggcaatc agtacacatc taaaggggta aaagccttta
     3361 tcaacccgct tttacaccac tacaagagca cgttacctca taccgagata ttggttcggg
     3421 gtgacagcgg cttcgccaca ccagaggtgt atgagtcttg tgaagcaact gaaagccagt
     3481 acgttatccg attaaagagc aatcggaggt taagtcagtt agctgaacat tctgttcttt
     3541 acggggacaa tcaagaatgg gaagagcgag aagtgcagta tttttcacta ccttatcaag
     3601 cacaatcctg gtcgaaacct cgtcgtgtct gtatacgctc cgtccgtgaa ggaggagaat
     3661 tgctatttca tcatgcgttt attgtgacta atctatccga caatgtctcg cctgaagtca
     3721 tattttctct ttacggcaag cgaggaacaa tggaaaattt catcaaagaa gcgaaaggtg
     3781 gcttttactt cgataaaaca gatagcccgc gtttcttaga aaatcatgtc agaatgatga
     3841 tcagtgtcct tgcatacaac ctcgttaatt ttttaaaaag cattggtttt gataaagtga
     3901 accagggcat gacgattcat tccatccggc tgatattgct taaagtagcc ggtaaacttg
     3961 ttcagactgg gagacaggtc tatctcaaac tgtcaagtta tcatgtgtat caaactgaat
     4021 tttataaggt ttttgaacgc ctgcggcgat ccaaacaatg gatttagact agttatcaca
     4081 caaatttttt aaccgattct ttttcaaggg attcgtctgc ccttaaatag acaaataatt
     4141 tttattatga tcttcttccg taggcatcct gttcgaaaac acattatttt ggaagaaagt
     4201 atctgcctaa tttaaaaatt ggcaatttgt tcaaaaaaac aggttaaatt tagagctatg
     4261 aattattcag gttttttcga ggtggaagct agtataaaag taaaccaagg g
//
LOCUS       DACCPY010000233         3540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.152, whole genome shotgun sequence.
ACCESSION   DACCPY010000233 DACCPY010000000
VERSION     DACCPY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.152"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(3..311)
                     /locus_tag="IVO69_002885"
     CDS             complement(3..311)
                     /locus_tag="IVO69_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401591.1"
                     /translation="MKNKLVSYAIMTVNNRYNRDYYSLFIPFVAETIRLKEFKYITPQ
                     IIKKEIQEKYDLEIPTAVINQIFNKLDKEKLIERKYYADQKKYLYHPVLLQSFKSTIK
                     "
     gene            complement(629..1588)
                     /locus_tag="IVO69_002886"
     CDS             complement(629..1588)
                     /locus_tag="IVO69_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7401592.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKVAKIAEFLKRSRTPIYNVIHF
                     LKEGHTALDYYQQYKENKKRCGRQKIILPKEQQTYIKEKVNQGWTPDVIIGRKEMIIS
                     CSMRTLYRQFKEKVFDEATLPMKGKRKPNGHQERRGKQAFKRNIVERKTDYPDFEEEF
                     GHIEGDTIVGAHHKSAIITLVERLSKVIITLKPDGRRASDIEATMDHWFNAIPRHLFK
                     SITFDCGKEFSNWKALSNQHDVAIYFADPGTPSQRGLNEHSNGLLRKDGLPKAMDFNL
                     VDQSFISSVADKRNNIPRKILNYQTPLEVFMSYMNKDILSSLI"
     gene            1797..2516
                     /gene="catA"
                     /locus_tag="IVO69_002887"
                     /pseudo
     CDS             1797..2516
                     /gene="catA"
                     /locus_tag="IVO69_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
     gene            3125..3526
                     /locus_tag="IVO69_002888"
     CDS             3125..3526
                     /locus_tag="IVO69_002888"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7401593.1"
                     /translation="MKNISLELKNIEVNFGNKELVAIKYLTVYENDRIGIVGRNGQGK
                     TTLLNLIQGKLSPNSGEVNRFVEFNYFKQMEETNNLYEFDDLNAELISRFTIPRNTIE
                     VLSGGEENKLRLARVLSNYKMGLLVLLQSFK"
BASE COUNT         1140 a          549 c          583 g         1268 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg gatgatataa gtatttcttc
       61 tgatccgcat aatattttct ttcaataagt ttttctttgt cgagtttatt aaatatttga
      121 tttattacag cagttggaat ttctaagtca tatttttctt gaatttcttt ttttattatc
      181 tgcggtgtta tgtatttaaa ttcttttaat ctaattgttt ctgcaacaaa tggtataaaa
      241 agagaataat aatctctgtt atatcgatta tttacagtca ttattgcata tgaaaccaat
      301 ttattcttca aaaagacaat ccctccataa aatgattata atactcttac tcatattata
      361 gtgattattc cttatgaatt ctatacgata gttattttaa tattataaat ttaattattt
      421 aatttattcc ttggttacaa taaaagtaat aatttctttt tattccttta tctcaaatta
      481 tttcttaatc ttatacacag tatctcttgc cacatctttt tcttcagata tcttcttgat
      541 tagctcctct tacttcaaat tattcatgat tacatgataa gtcattcgat cttcatattt
      601 tttatccctt ttttaatttt ttgatttgtc aaattaagct agacaaaata tctttattca
      661 tgtaactcat aaatacttcc aagggcgttt ggtaattcaa tatttttcta gggatgttgt
      721 ttcgtttgtc tgcaacagaa gaaatgaaac tttggtccac cagattgaaa tccatcgcct
      781 taggcaatcc gtcttttcgc aagagaccgt tggaatgttc attcagacca cgttgcgaag
      841 gtgttcctgg gtcagcaaaa tagatggcaa cgtcgtgttg gttacttaaa gctttccagt
      901 tagagaattc ttttccgcag tcaaaagtga tcgatttaaa taaatgcctt ggaatggcat
      961 taaaccaatg atccatggta gcttcaatat cgctggctct ccgcccatcg ggcttcaaag
     1021 taataataac cttagaaaga cgttctacca aggtaatgat ggcgctcttg tgatgagccc
     1081 ccacaatagt gtcgccttcg atatgaccga actcttcttc gaagtcagga taatcggtct
     1141 ttctttcaac aatattccgt ttgaaggctt gttttcctct gcgttcttga tgaccattgg
     1201 gttttctttt ccctttcata ggaagtgtag cttcatcgaa gactttttct ttaaactggc
     1261 ggtaaagagt ccgcatagaa cagctgatga tcatttcttt tcgtccaata atgacatcag
     1321 gagtccagcc ttgatttact ttttccttga tataggtctg ttgttctttt ggtaagatga
     1381 ttttctgtct gccacaccgt ttcttatttt ctttatattg ttggtagtaa tcgagggccg
     1441 tatggccttc tttcaagaaa tgaatgacat tataaatggg tgttcgagat cgtttcaaga
     1501 actcagcaat tttagcgact ttaatatttt ggtgataata agactctatc atcactaatt
     1561 catccgtggt aagatgggtg taggtcattc gtgatcactc ctatgttttc tttggtcgga
     1621 aatacatttt gagtgtacca cgaatggctt ttttatttgt ctagcttaat tttacaatcg
     1681 gcgtatattt atgttacaat aatactgact tttaaaaaag gattgattct aatgaagaaa
     1741 gcagacaagt cagcctccta aattcacttt agataaaaat ttaggaggca tatcaaatga
     1801 actttaataa aattgattta gacaattgga agagaaaaga gatatttaat cattatttga
     1861 accaacaaac gacttttagt ataaccacag aaattgatat tagtgtttta taccgaaaca
     1921 taaaacaaga aggatataaa ttttaccctg catttatttt cttagtgaca agggtgataa
     1981 actcaaatac agcttttaga actggttaca atagcgacgg agagttaggt tattgggata
     2041 agttagagcc actttataca atttttgata gtgtatctaa aacattctct ggtatttgga
     2101 ctcctgtaaa gaatgacttc aaagagtttt atgatttata cctttctgat gtagagaaat
     2161 ataatggttc ggggaaattg tttcccaaaa cacctatacc tgaaaatgct ttttctcttt
     2221 ctattattcc atggacttca tttactgggt ttaacttaaa tgtcaataat aatagtaatt
     2281 accttctacc cattattaca gcaggaaaat tcattaataa aggtaattca atatatttac
     2341 cgctatcttt acaggtacat cattctgttt gtgatggtta tcatgcagga ttgtttatga
     2401 actctattca ggaattgtca gataggccta atgactggct tttataatat gagacaatgc
     2461 cgactgtact ttttacagtc agttttctaa tgtcactaac ctgcccgtta atttaagtgt
     2521 actacttcac aaaacgcatt gcttattaca tatgtaataa gcaatgccac aactcttaag
     2581 ttaagactat aatgacaaat gtaactaata atatttttta cggtggatta tataatgaag
     2641 ttagccaccg ctaactaaaa aacgccatgt gaatttcatg ttatattgaa gttaccttga
     2701 atggcgaaaa aggagcaaga gtattttttt tcatatcacc tattataagt tatcttgacc
     2761 tataataggt gagcataact tgactatttg aaaaaaatgt tttaagcttt atgagacgtt
     2821 ggtagaaaga tacgtgttca tttgagcagt atctagtata caaataggat gtacaaagga
     2881 gaatttggaa aatgacagca tctataagat tgtggtttaa ccttttgaaa taataattaa
     2941 atactattat tcaaaaggtc ctgttatatt ggcagaaaca ggggagcaat ctgtctattt
     3001 ttcaaattct atttatctaa gaaaatatag atatggaaat tgtcaaaaga ggtagaaatc
     3061 tatctctttt ttgtgtaccc aaaaatctgt tgattgcacg gcctaattta aggagtgcaa
     3121 aataatgaaa aatatatctt tagaattaaa aaatatagaa gtaaattttg gaaacaaaga
     3181 actagtagcc attaaatatt taactgtata tgagaatgat cgaattggca ttgtcggaag
     3241 aaacggacaa gggaaaacaa cgctactgaa tcttatccaa ggaaagttat caccaaattc
     3301 aggggaagtt aaccgatttg tggagtttaa ttactttaaa cagatggaag aaaccaataa
     3361 tttatacgaa tttgatgatc ttaacgctga acttattagt cgattcacta ttccgagaaa
     3421 tacgatcgaa gtactaagcg gaggtgaaga aaataaatta cgtttagcta gagtgctatc
     3481 aaattataag atgggattac tggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DACCPY010000234         3277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.155, whole genome shotgun sequence.
ACCESSION   DACCPY010000234 DACCPY010000000
VERSION     DACCPY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.155"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            276..773
                     /gene="dfrG"
                     /locus_tag="IVO69_002889"
     CDS             276..773
                     /gene="dfrG"
                     /locus_tag="IVO69_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7401594.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(845..2797)
                     /locus_tag="IVO69_002890"
     CDS             complement(845..2797)
                     /locus_tag="IVO69_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7401595.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2804..3100)
                     /locus_tag="IVO69_002891"
     CDS             complement(2804..3100)
                     /locus_tag="IVO69_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7401596.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1015 a          566 c          539 g         1157 t
ORIGIN      
        1 gtaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt
       61 ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
      121 gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa
      181 ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga aataagaaat
      241 tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga
      301 tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact
      361 gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg
      421 aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt
      481 ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa
      541 acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg
      601 ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag
      661 aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa
      721 atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc
      781 gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt
      841 tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc
      901 tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt
      961 aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat
     1021 agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc
     1081 ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga
     1141 tttcgtttgg ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat
     1201 ccttagtggc taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat
     1261 ctttactaat agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag
     1321 atgccaaagt ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt
     1381 ctgcttttgc ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt
     1441 ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg
     1501 cgtttctaat agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt
     1561 gtttaattaa ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga
     1621 caaatctact cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc
     1681 tatcgttagc ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac
     1741 taaaagtatt gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg
     1801 aaatattcca atcataggca acatcagaac ggaacacata tcttgataag acctcagcag
     1861 atacaactat atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt
     1921 gtcctttctt acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct
     1981 cgttaaattt ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac
     2041 gcctatacag ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac
     2101 aagtacgact gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt
     2161 tcttttgttg gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct
     2221 tacacgcctt acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt
     2281 gcttcggctc attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat
     2341 aatctggctc aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa
     2401 cagaccaatt agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat
     2461 tgtcattgca gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc
     2521 gacttggttt cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa
     2581 acggattgat gcagtaatta tattgaatgg gatattcaga accgttccat ttaaaagacg
     2641 ttggcaagaa caacattttg ttataccttg ttgcaaatcg ttttggagtt aataaatcat
     2701 attgtttagc cctatctttt aattcttcgt ttgaaatcgg aattttaaca tctacaattt
     2761 catcttcctt cgtggctaat cttttaagct tggtcattgt ctatcaaccc actctatctt
     2821 tgatacccat tttttcaaca atccgcttat tacttcgctt gttggcaatc cattcaataa
     2881 acttcttatc gtctaaaatg ttcaataaga attcatccac tgcttcgctt tcagtacgct
     2941 ctgcatattc agcgtattgc ttaactatca cccttacttg ctctgatatt tcccaatcca
     3001 cagacttagc gttcttattt tttggctcaa gaaattgcat aggaaaatgc accccttttt
     3061 gcttagtaaa ttgtaaccat tttaacacac caatttgcat tcctacaata attatttcac
     3121 gttcgcattt ttatatacat tttaacttac attttgtggt gtaaattaac aacaaacgtt
     3181 gatttaacaa taaaaaacga gtatcaaatt cagttgcaat ttgatactca tttaggttta
     3241 cattttcact ttttgtactc caaaactgaa ctactta
//
LOCUS       DACCPY010000235         3099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.157, whole genome shotgun sequence.
ACCESSION   DACCPY010000235 DACCPY010000000
VERSION     DACCPY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.157"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            74..190
                     /locus_tag="IVO69_002892"
     CDS             74..190
                     /locus_tag="IVO69_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7401597.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            206..2125
                     /gene="tet(M)"
                     /locus_tag="IVO69_002893"
     CDS             206..2125
                     /gene="tet(M)"
                     /locus_tag="IVO69_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7401598.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYDDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRNDLTFFTNGRSVCLTELKGYQVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2240..2290
                     /locus_tag="IVO69_002894"
                     /pseudo
     CDS             2240..2290
                     /locus_tag="IVO69_002894"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2324..>3099
                     /locus_tag="IVO69_002895"
     CDS             2324..>3099
                     /locus_tag="IVO69_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7401599.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTALYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FPILILARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPF"
BASE COUNT         1024 a          482 c          622 g          971 t
ORIGIN      
        1 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
       61 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      121 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      181 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      241 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      301 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      361 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      421 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      481 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      541 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      601 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctatggaaat
      661 tataatcaaa cagaaagtag agctgcaccc taatatgtgt gtgatgagct gtacggaacc
      721 tgagcaatgg gatgtggtaa tagaaggaaa tgatgacctt ttagagaaat atatgtccgg
      781 taaatcatta gaagcattag aactcgaaca agaggaaatc agaagatttc agaattgctc
      841 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
      901 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
      961 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     1021 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     1081 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     1141 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     1201 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     1261 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     1321 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     1381 gaaagtacaa atggaagtga cttgtgctct attgcaagaa aagtatcatg tggaggtaaa
     1441 aataaaaaag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     1501 tcacatcgaa gtgccaccga atcccttctg ggcttccatt ggtttatctg tatcaccgct
     1561 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     1621 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     1681 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     1741 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagttttaa aaaaagctgg
     1801 aacagaattg ttagagccat atcttagttt taaaatttat gcaccacaag aatatctttc
     1861 acgagcatat gacgatgctc ctaaatattg tgcgaacatc gtagacactc aactgaaaaa
     1921 taatgaggtc attcttagtg gagaaattcc tgctcggtgt attcaagaat atcgtaatga
     1981 tttaactttc tttacaaatg gacgtagcgt ttgtttaaca gagttaaaag gatatcaggt
     2041 taccactggc gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     2101 atatatgttc aataaaataa cttagcacat tttatattgt tatataaatg tggcttcttg
     2161 ttaaacgaaa cgagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     2221 attaattatg aagtaaaatg aatgtaacag ggttcaatta aaagagggaa gcgtatcatt
     2281 aaccctataa actacgtctg ccctcattat tggagggtga aatgtgaata catcctattc
     2341 acaatcgaat ttacgacaca accaaatttt aatttggctt tgcattttat ctttttttag
     2401 cgtattaaat gaaatggttt tgaacgtctc attacctgat attgcaaatg attttaataa
     2461 accaccggcg agtacaaact gggtgaacac agcctttatg ttaacctttt ctattggaac
     2521 agctctatat ggaaagctat ctgatcagct aggcattaaa aggttactcc tatttgggat
     2581 tataataaat tgcttcggat cggtaattgg gtttgttgga cattctttct ttcccatact
     2641 tattctggct cgatttattc aaggagctgg tgcagctgca tttccagcac tcgtgatggt
     2701 tgtagttgcg cgctatattc caaaggaaaa taggggtaaa gcatttggtc ttattggatc
     2761 gatagtagcc atgggagaag gagtcggtcc agcgattggt ggaatgatag cccattatat
     2821 tcattggtcc tatcttctac tcattcctat gataacaatt atcactgttc cgtttcttat
     2881 gaaattatta aagaaagaag taaggataaa aggtcatttt gatatcaaag gaattatact
     2941 aatgtctgta ggcattgtat tttttatgtt gtttacaaca tcatatagca tttcttttct
     3001 tatcgttagc gtgctgtcat tcctgatatt tgtaaaacat atcaggaaag taacagatcc
     3061 ttttgttgat cccggattag ggaaaaatat accttttat
//
LOCUS       DACCPY010000236         2536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.168, whole genome shotgun sequence.
ACCESSION   DACCPY010000236 DACCPY010000000
VERSION     DACCPY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.168"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            225..1703
                     /gene="msr"
                     /locus_tag="IVO69_002896"
     CDS             225..1703
                     /gene="msr"
                     /locus_tag="IVO69_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7401600.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1750..2196
                     /locus_tag="IVO69_002897"
     CDS             1750..2196
                     /locus_tag="IVO69_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7401601.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2395..>2536)
                     /locus_tag="IVO69_002898"
     CDS             complement(2395..>2536)
                     /locus_tag="IVO69_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7401602.1"
                     /translation="KKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQF
                     FE"
BASE COUNT          826 a          425 c          531 g          754 t
ORIGIN      
        1 taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga
       61 acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt
      121 tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta
      181 gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag
      241 taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc
      301 ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga
      361 ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga
      421 tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg
      481 aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag
      541 gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc
      601 tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc
      661 gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag
      721 catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg
      781 cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca
      841 tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt
      901 tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag accggatcgg ttgtcttcct
      961 ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa
     1021 gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag
     1081 taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg
     1141 aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa
     1201 tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac
     1261 aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt
     1321 atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag
     1381 aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac
     1441 cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta
     1501 agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg
     1561 aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt
     1621 cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa
     1681 aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag
     1741 tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt
     1801 ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca
     1861 agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc
     1921 atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc
     1981 caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta
     2041 ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct
     2101 tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca
     2161 agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag
     2221 gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga
     2281 acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt
     2341 attgaagata atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc
     2401 aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag
     2461 cataattctg cgctggatca caacataacg tccaaattcc tgtttcttgt ctagcgagag
     2521 gtcattcaat tttttt
//
LOCUS       DACCPY010000237         1782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.180, whole genome shotgun sequence.
ACCESSION   DACCPY010000237 DACCPY010000000
VERSION     DACCPY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.180"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..616)
                     /locus_tag="IVO69_002899"
     CDS             complement(<1..616)
                     /locus_tag="IVO69_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7401603.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRL"
     gene            1169..1717
                     /gene="aac(6')"
                     /locus_tag="IVO69_002900"
     CDS             1169..1717
                     /gene="aac(6')"
                     /locus_tag="IVO69_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7401604.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          580 a          310 c          352 g          540 t
ORIGIN      
        1 agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat
       61 catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg
      121 cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag
      181 gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata
      241 ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta
      301 gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga
      361 cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat
      421 tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt
      481 tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt
      541 ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga
      601 aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa
      661 gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt
      721 gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag
      781 tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt
      841 ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca
      901 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
      961 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
     1021 aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat
     1081 actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta
     1141 gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc
     1201 agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga
     1261 cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt
     1321 agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg
     1381 ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt
     1441 agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac
     1501 ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga
     1561 taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg
     1621 ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat
     1681 ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt
     1741 aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
LOCUS       DACCPY010000238         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.210, whole genome shotgun sequence.
ACCESSION   DACCPY010000238 DACCPY010000000
VERSION     DACCPY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.210"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            13..378
                     /locus_tag="IVO69_002901"
     CDS             13..378
                     /locus_tag="IVO69_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401605.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002902"
     CDS             379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP7401606.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT          419 a           82 c          185 g          355 t
ORIGIN      
        1 attgatgata atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt
       61 tcatttgggt ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca
      121 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
      181 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      241 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      301 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      361 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      421 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      481 gatggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      541 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      601 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      661 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      721 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      781 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      841 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      901 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      961 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
     1021 gtggcatatt tagttaataa t
//
LOCUS       DACCPY010000239         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.211, whole genome shotgun sequence.
ACCESSION   DACCPY010000239 DACCPY010000000
VERSION     DACCPY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.211"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            13..378
                     /locus_tag="IVO69_002903"
     CDS             13..378
                     /locus_tag="IVO69_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7401607.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002904"
     CDS             379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO69_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP7401608.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT          418 a           82 c          186 g          355 t
ORIGIN      
        1 attgatgata atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt
       61 tcatttgggt ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca
      121 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
      181 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      241 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      301 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      361 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      421 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      481 ggtggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      541 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      601 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      661 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      721 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      781 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      841 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      901 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      961 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
     1021 gtggcatatt tagttaataa t
//
LOCUS       DACCPY010000240          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.218, whole genome shotgun sequence.
ACCESSION   DACCPY010000240 DACCPY010000000
VERSION     DACCPY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.218"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            117..851
                     /gene="erm"
                     /locus_tag="IVO69_002905"
     CDS             117..851
                     /gene="erm"
                     /locus_tag="IVO69_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP7401609.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYTKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          382 a          120 c          130 g          295 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactc aaccaaacaa aaaataagtg
       61 gttataatgg attgttaata taaacattca ttataacctc ataggagtgg gttataatga
      121 acaaaaaaaa tataaaagat agtcaaaact ttattacttc aaagcatcat ataaatgaaa
      181 ttttgagaaa tgtacattta aatacaaatg ataatattat tgagattggt tcagggaaag
      241 gtcatttcac gtttgaatta gctaaaaggt gtaattatgt aaccgccatt gaaatagatc
      301 ctaaattatg taggataact aaaaacaaac ttattgaata tgagaacttc caggttatca
      361 ataaagatat tttacaattt aagtttccta aaaataagtc atataagata tatggaaata
      421 taccctacaa tataagtaca gatataattc gaaaaattgt ttttgaaagc acagctacag
      481 aaagttattt aatagtggaa tatggatttg ctaaaaggtt gctaaataca aatcgttcac
      541 tagcactatt tttaatgaca gaagttgata tatccatatt aagtaaaatc cctagagaat
      601 actttcatcc aaaacctaga gttaatagct cgttaattgt attaaaaaga cacccttcaa
      661 aaatatcact caaagataga aaacaatatg aaaattttgt tatgaaatgg gttaacaaag
      721 aatacacaaa actattttcc aaaaaccaat tttatcaagc cttaaaatat gcaagaattg
      781 acgatttaaa caatattagc tttgaacaat tcttgtctct tttcaatagc tataaattat
      841 ttaatagata agttaagggg tgcataaact gcatccctta acttatctgg ttctgttgca
      901 aagttttaaa tctactatca aataagg
//
LOCUS       DACCPY010000241          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_075
            SAMN12916532-rid10366883.guided.219, whole genome shotgun sequence.
ACCESSION   DACCPY010000241 DACCPY010000000
VERSION     DACCPY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916532
            Sequence Read Archive: SRR10233607
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820162.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:26:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,867
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916532-rid10366883.guided.219"
                     /isolate="RPH_EFM_075"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..817
                     /locus_tag="IVO69_002906"
                     /note="aph(2'')-Ia"
     CDS             <1..817
                     /locus_tag="IVO69_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7401610.1"
                     /translation="GSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLE
                     TNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMH
                     GLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKK
                     CLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDIL
                     RMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          395 a           59 c          148 g          300 t
ORIGIN      
        1 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
       61 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      121 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      181 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
      241 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
      301 aatgcacggt ttagattata cagatattag tgaatgtact attgataata aacaaaatgt
      361 attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa
      421 agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa
      481 gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt
      541 aactggaata attgattttg gagattctgg aattatagat gaatattgtg attttatata
      601 cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat
      661 gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta
      721 tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg
      781 tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat gaaaagctat
      841 tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag gaagtgagtc
      901 aa
//
